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  Bionconductor http://www.bioconductor.org/
The Bioconductor project produces an open source software framework that will assist biologists and statisticians working in bioinformatics, with primary emphasis on inference using DNA microarrays, built mostly using R.
  AcePerl http://stein.cshl.org/AcePerl/
OO Interface to AceDB
  Bioperl.org http://www.bioperl.org
The Bioperl Project is an international association of developers of open source Perl tools for bioinformatics, genomics and life science research. The Bioperl server provides an online resource for modules, scripts, and web links.
  BioJava.org http://biojava.org/
The BioJava Project is an open-source project dedicated to providing Java tools for processing biological data.
  AceDB http://www.acedb.org/
A hierarchical database system for displaying genomic data (originally C. elegans), suitable also for displaying other scientific data.
  Pathway Tools http://bioinformatics.ai.sri.com/ptools/
Predict metabolic pathways from genomes and graphically depict pathways. Visualize gene expression data on a metabolic pathway map. Generate a metabolic wall chart. Includes a genome browser and support for transcriptional regulatory networks.
  PISE http://www.pasteur.fr/recherche/unites/sis/Pise/
Given an abstract definition of a program's parameters, PISE can generate whatever style of interface from the configuration module.
  BioPython http://biopython.org/
The Biopython Project is an international association of developers of freely available Python tools for computational molecular biology.
  The XEMBL Project http://www.ebi.ac.uk/xembl/
XEMBL provides access to the EMBL/GenBank/DDBJ Nucleotide Sequence Database, providing output in various XML standards.
  Dynamite http://www.ebi.ac.uk/Dynamite/
Code generator for writing dynamic programming programs.
  BioWarehouse Database Integration System http://biowarehouse.ai.sri.com/
Integrates multiple public bioinformatics databases into a single relational database system within a common bioinformatics schema.
  Boulder.pm http://www.broad.mit.edu/genome_software/other/boulder.html
Simple but powerful data exchange language, suitable for serializing biological data objects.
  Bielefeld University Bioinformatics Server http://bibiserv.techfak.uni-bielefeld.de/
BiBiServ supports Internet-based collaborative research, software development and distance education in bioinformatics.
  Cell Electrophysiology Simulation Environment (CESE) http://cese.sourceforge.net/
A framework to perform electrophysiological simulations. Useful for simulations of action potentials, individual ionic currents, and changes in ionic concentrations.
  MolTalk http://www.moltalk.org/
Software development for structural bioinformatics. Runs on Unix, Windows and MacOSX.
  CLEML: Carbon Labeling Experiment Markup Language http://sg.ustc.edu.cn/MFA/cleml/
CLEML open format for representing and exchanging 13C metabolic flux analysis models.
  InterMine http://www.intermine.org
A general-purpose object-oriented data warehouse system developed as part of the FlyMine project and made available as stand-alone open-source software.
  The Scriptome http://sysbio.harvard.edu/csb/resources/computational/scriptome
A set of software tools that filter, format, and merge data in tabular or common biological formats. Organized as a classified collection of Perl one-liners.
  BioPHP http://www.biophp.org
An open source project to develop PHP code and scripts for biocomputing. A wiki-like service allows collaboration between developers.
  BioLisp.org http://nostoc.stanford.edu/Docs/
BioLisp.org is a public resource supporting scientists who use Lisp to develop intelligent applications in the biological sciences. We collect and disseminate Lisp biocomputing code, and gather pointers to Lisp and other Intelligent BioComputing methods.
  NeoBio http://neobio.sourceforge.net
An open source library of Java bioinformatics algorithms. The current version consists mainly of sequence alignment algorithms such as Needleman- Wunsch and Smith-Waterman.
  The Darwin Manual http://www.inf.ethz.ch/personal/gonnet/DarwinManual/DarwinManual.html
Guide to running this programming language. Library contains programs to compute pairwise alignments, phylogenetic trees, multiple sequence alignments, and to make secondary structure predictions.
  BioQuery http://www.bioquery.org/
Tools that searches multiple biomedical databases from your desktop.
  JCell http://www-ra.informatik.uni-tuebingen.de/software/JCell/
A Java based framework to reconstruct genetic interactions from microarray data.
  Biochemical Algorithms Library http://www.bioinf.uni-sb.de/OK/BALL/
C++ framework for rapid software prototyping in molecular modeling.
  High Throughput Screening Tools http://www.htstools.com/
Analytical tools and SQL scripts that solve common problems in drug discovery.
  EST2uni http://www.melogen.upv.es/genomica/web_estpipe/index.php
A tool to analyze a set of ESTs. Cleans, clusters and annotates the sequences and prepares a web site to browse the results in a complete automatic way.
  Sight http://bioinformatics.org/jSight/
Extendable parallel system for automatic genome analysis. Software for analysing all potential genes in a defined genome region. Given two marker names, can output reports of genomic features between them. Features for converting between naming systems, sequence retrieval, E-PCR, gene prediction, similarity search, protein pattern search, and report formatting. Written in Java.
  NCICB Core Infrastructure http://ncicb.nci.nih.gov/NCICB/infrastructure
The National Cancer Institute's Center for Bioinformatics produces the caCORE infrastructure, an open-source enterprise architecture for cancer informatics.
  Phylogenetic Analysis Library http://www.cebl.auckland.ac.nz/pal-project/
PAL library is a Java toolkit for determining evolutionary trends.
  BioRuby http://bioruby.org
BioRuby project aims to implement an integrated environment for Bioinformatics with Ruby language.

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