T4 DNA ligase activity measurements are based on the use of radioactivity or rather labor-intense procedures including gel-based analysis. We therefore ...
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Checking Your T4 DNA Ligase Activity. See Navigation. ‹ Molecular Biology ... DNA Ligase Activity. Troubles with ligation? Check the activity of your T4 DNA ...
Altered DNA ligase activity in human disease. Mutagenesis. 2020 Feb 13;35(1):51-60. doi: 10.1093/mutage/gez026. Authors. Alan E Tomkinson , Tasmin Naila , ...
GO:0003909 JSON. DNA ligase activity. Molecular Function. Definition (GO:0003909 GONUTS page). Catalysis of the formation of a phosphodiester bond between ...
DNA ligase activity requires a nucleotide cofactor and follows a unique three-step reaction mechanism that involves covalent modification of the ligase ...
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Tth DNA ligase activity is assayed in a reaction containing 1 µg of bacteriophage lambda DNA digested with SalI and SmaI (Control DNA), 1x Tth Ligation ...
Sep 6, 2008 · This study presents a simple, label-free electrochemical technique for the monitoring of DNA ligase activity. DNA ligases are enzymes that ...
Mar 18, 1982 · ... DNA ligase activity. We suggest that ADPRT has a general function in the control of the breaking and rejoining of DNA.
Control Reaction for T4 DNA Ligase Activity. Unsuccessful ligation may be a result of inactivated ligase or inhibition of ligation reaction by impurities in ...
Jun 15, 1995 · A DNA metalloenzyme with DNA ligase activity · 2231 Accesses · 352 Citations · 6 Altmetric · Metrics details ...