CA1151107A

CA1151107A - Reference elements for ion-selective membrane electrodes

Info

Publication number: CA1151107A
Application number: CA000355316A
Authority: CA
Inventors: Sang H. Kim
Original assignee: Eastman Kodak Co
Current assignee: Eastman Kodak Co
Priority date: 1979-08-13
Filing date: 1980-07-03
Publication date: 1983-08-02
Also published as: DE3067346D1; EP0024191A2; JPS5633538A; EP0024191B1; US4263343A; EP0024191A3

Abstract

Abstract of the Disclosure A reference element for ion-selective membrane electrodes is prepared by coating portions of a layer or silver with a composition comprising a metal salt elec-trolyte, a hydrophilic binder and a member of the group consisting of an oxidizing agent and a silver halide emul-sion.

Description

R~FERENCE ELEMENTS FOR ION-SELECTIVE
MEMBRANE ELECTRODES
This application relates to a process ~or prepar-ing a reference element for ion-selective membrane elec-trodes and ion-selective membrane electrodes containing these elements.
The use of electrodes for the measurement of various ionic solutions is widespread. Typically, devices for obtaining such measurements include a reference elec-trode and a separate ion-selective electrode. When simul-taneously contacted with the body of solution to be anal-yzed, the reference and ion-selective electrodes constitute an electrochemical cell, across which a potential develops.
Measurement of the potential determines the concentration of ions in the solution.
One useful reference element for ion-selective electrodes comprises a metal in contact with an insoluDle salt of the metal which is in turn in contact with an electrolyte, i.e., a solution containing the anion of the salt. A very commonly used example of such a reference element can be represented as Ag/AgCl/"XMCl " (XMCl indicating a solution of known Cl concentration) and comprises a silver wire having a coating of silver chloride applied thereto by dipping into an aqueous solution of known chloride concentration~
The silver halide layer of the reference electrode is conventionally coated on the silver substrate by contact-ing the silver substrate with an oxidizing agent and drying prior to overcoating with an electrolyte layer.
In U.S. Patent 4,214,968 of Battaglia et alg issued ,Tuly 293 1980, a reference element is described which is formed by coating a support such as poly(ethylene tere-phthalate) with a metallic silver layer, such as ~y plating techniques, and treating the silver layer overall with an oxidizing agent or a silver halide emulsion and drying. m e surface of the silver layer is thus converted to or coated .~
? `

'7 by silver halide. The layer is dried and the silver halide layer is overcoated with an electrolyte layer com-prising the metal salt forming the electrolyte and a hydro-philic binder. The resulting reference element ls a sub-strate overall coated with a silver layer, overall coatedwith a silver halide layer and overall coated ~ith the electrolyte layer~ A membrane layer is added and the resulting ion-selective electrode is completed by connecting a probe through the various layers to the silver layer for contact.
Although the above method results in an acceptable reference element, the method is costly, as it involves a plurality of steps, and the electrical contact of the probe with the silver layer is not always good, as the probe must be forced through the membrane, electrolyte and silver halide layers to make contact with the silver layer.

Summary of the Invention According to the present invention, there is pro-vided a novel method for preparing a metal/silver halide reference element comprising coating portions of a metal layer with a metal salt electrolyte, a hydrophilic binder and a member of the group consisting of an oxidizing agent and a silver halide emulsion.
This process i3 relatively inexpensive, as it requires only one step in coating the metal layer to achieve the metal layer having coated thereover a silver halide layer and an electrolyte layer, and the resulting electrode contains uncoated portions of silver which can be easily contacted with a probe.
According to a further embodiment of the present invention9 an ion-selective electrode is prepared by overall coating a support with a metal, coating portions of the metal with a composition comprising a metal salt electro-lyte, a hydrophilic binder and a member selected from the group consisting of an oxidizing agent and a silver halide emulsion and overcoating with a hydrophobic membrane layer containing an ionophore and an ion-carrier solvent and a :; :

)'7 hydrophobic binder. The membrane layer is useful in electrodes which measure ions3 such as potassium, carbonate and the like.
In a further embodiment of this invention, a metal/silver halide reference element comprises a support containing a layer of metal, portions of said layer of metal having thereon a composition comprising a metal salt elec-trolyte, a hydrophilic binder and a member of the group consisting of an oxidizing agent and a sllver halide emul-sion; portions of said layer of metal being free from saidcomposition.

Description of the Drawing The drawing shows a cross-sectional view of an ion-selective element as described herein.

Description of the Preferred Embodiments The reference elements of the present invention are prepared by coating portions of a metal layer with a composition comprising a metal salt electrolyte, a hydro-philic binder and a member of the group consisting of an oxidizing agent and a silver halide emulsion.
The metal layer need only comprise a conductive metal such as silver, nickelg copper and the like. The preferred metal is silver. The metal layer can either be self-supported or coated on a support. Suitable supports for a silver layer are preferably insulating and include glass, paper and polymeric supports, such as polyesters such as poly(ethylene terephthalate~, cellulose ester materials and the like.
The metal layer can be formed in situ or coated onto a support using any suitable method of depositing the metal. In the case of silver, the silver layer, pre-ferably a thin layer, can be formed by electroless deposi-tion, vacuum-depositing silver, depositing a photographic silver halide layer and exposing and developing to form silver, and the like.
Portions of the metal layer are then coated with the composition containing the metal salt electrolyte, oxidizing agent or silver halide emulsion and hydrophilic binder~ The coating supplies not only the silver halide layer, but also the electrolyte layer of the reference ele-ment.
The composition can be coated onto the metal uslng any conventional coating technique. The coating ls applied, however, to only a portion of the metal layer, leaving the remainder of the layer uncoated. In a preferred embodiment, the composition is applied in stripes to the metal layer by roll coating, dipping, laminating, brush coating or other coating techniques. After application, the coating is dried, preferably at ~20C to ~95C for 1 to 30 minutes The resulting reference element comprises the support coated with a metal layer which is then stripe-coated with a silver halide layer coated with the electrolyte layer.
By "coating portions of a metal layer" it is meant that the metal layer would, in some areas, be coated and in other areas be uncoated. The most preferable method of partially coating the metal layer is to coat in a striped fashion. That is, the coating is applied in individual striped areas leaving the remainder of the metal layer uncoated. Likewise, when a membrane layer, and opt~onally an overcoat layer, is applied, it is applied in the same areas as the reference coating, so that the same portions of the metal layer remain uncoated.
The coating composition corr.prises a metal salt electrolyte in solid solution with a hydrophilic binder. In a preferred embodiment, the portion of the cation of said salt comprises the ion which the electrode is designed to detect. Typically, the binder and salt are in solution with a solvent for both.
The binder for the electrolyte solution may com-prise any hydrophilic material suitable for the formation of continuous, coherent, cohesive layers compatible with the salt of the electrolyte layer and, if formed by coating, a solvent for both the ionic salt and the polyrneric binder.
Preferred materials of this type are hydrophilic, natural and synthetic polymeric film-forming materials, such as ~ t7 polyvinyl alcohol, gelatin, a~arose, deionized gelatin, polyacrylamide, polyvinyl pyrrolidone, hydroxyethyl acry-late, hydroxyethyl methacrylate, polyacrylic acid, etc.
Specifically preferred from among these materials are the hydrophilic colloids, such as gelatin (especially deionized gelatin), agarose, polyvinyl alcohol and hydroxyethyl acrylate.
The ionic salt which is dissolved in the polymeric binder solution will be determined by the composition of the Ag/Ag halide layer to be formed. For example, in a potassium-selective electrode which uses AgCl a~ the in-soluble metal salt, potassium chloride is a logical choice, although sodium chloride, etc, may also be used. For sodium ion determinations in a similar conflguration, sodium chloride would be useful, etc. Thus, the salt will gen-erally be a water-soluble salt having a cation selected from ammonium, alkali metals and alkaline earth metals, mixtures of the same or any other suitable cation to which the electrode responds, and as anion a halogen or sulfur, depending upon the composition of the metal-salt layer.
Conductive metal salts of these anions are commonly in-soluble.
The composition also comprises a member selected from the group consisting of an oxidizing agent and a silver halide emulsion. The oxidizing agent can be any material whose electrode potentials are more positive than standard electrode potentials of the metal being coated (Ag + e =
Ag, 0.7996V).
Examples of useful oxidizing agents are KClCrO3, K3Fe(CN)6, KMnO4, K2Cr207, NH4V03, (NH4)2Ce(N03)6, Fe(C204)3 and the like. Preferred oxidizing agents are KClCrO3 and K3Fe(CN)6. Combinations of oxidizlng agents can be used. A more thorough listing of oxidizing agents useful herein can be found in Handbook _ Chemistry and Physics, 50th Edition, The Chemical Rubber Company, 1969, pp D109-114.
The amount of oxidizing agent used can vary de-pending on its oxidizing power, but preferably the coverage ` ~ 5~ ~'7 should be between 0.01 and 1.0 g/m2. If an oxidizing agent is used, the metal conducting layer should be silver.
The composition can alternatively comprise a silver halide emulsion to effect the silver halide layer.
In such case, the silver halide is in the form of an emul-sion with the hydrophilic binder in the coating composition and the metal conducting layer can be other than silver.
The silver halide can comprise silver chloride, silver bromide, silver iodide, silver bromoiodide and the like.
Varying coverages of silver halide and a hydrophilic binder such as gelatin can be used, but preferably the coverage of silver halide is from 1.16 to 1.83 g/m2 of silver as silver halide in 0.054 to 0 54 g/m2 gelatin.
The composition to be coated over the metal layer can generally comprise from about 0.1 to about 7 5 g/m2 of metal salt electrolyte and from about 0.5 to about 10 g/m2 of hydrophilic binderr Generally~ salt concentrations of from about 30% to about 50% by weight binders in the layer are preferred.
The coating composition can also contain other addenda, such as surfactants, for example saponin, Surfac-tant 10G and the like; buffering agents, such as NaOH, HCl, phosphate, acetic acid and the like.
Appropriate solvents for the polymeric binder and ionic salt will depend largely on the nature of the polymer and the salt. Generally, polar solvents suitable for dis-solving the salt and the polymer are satisfactory. Thus, water is a preferred solvent for layers of hydrophilic materials~ such as polyvinyl alcohol and gelatin.
The coating composition can be formulated by merely adding the mçtal salt electrolyte and oxidizing agent or silver halide emulsion to a hydrophilic binder, such as gelatin, in the presence of water and drying.
Since the thickness of the 'Idried'' electrolyte layer will, to some extent, determine the response char-ac-teristics of the electrode, it is generally desirable to maintain the "dried" layer rather thin. Layers having dry thicknesses on the order of from about 0.1 to about 0.5 mil 3'7 have been ~ound useful. A preferred thickness is about 0.2 mil. Of course, where electrode response characteristics are not critical, the thickness of the layer may vary over a wide range. The application of sound engineering skills 5 and the use requirements of the finished electrode will determine its llmits.
The reference elements described above are particularly useful in dry operative ion-selective electrodes, which require a membrane layer containing an 10 ionophore.
The electrodes generally can comprise the reference element described above in a conventional solution electrode such as a barrel electrode or can be in the form of a dry operative electrode (both as described in Research Disclosure 16113, published by Industrial Opportunities Limited, Homewell, Havant, Hampshire, PO9, lEF, UK, Volume 161, September, 1977). Solution assays can be carried out, for example, in barrel type electrodes containing electrode bodies having therein a membrane.
20 The sample is contacted to the membrane and a reference electrode is inserted into the electrode body.
Dry operative electrodes are those described in U.S. Patent 4,214~968 of 3attaglia et al, issued July 29, 1980~ and comprise a reference electrode layer coated with 25 an electrolyte layer and a membrane layer.
The membrane of the electrode designed to measure potassium, sodium, CO2, and other ions requiring a membrane can be coated over the reference element by any means, such as roll coating, dip coating and the like.
The ion-selective membrane can be any membrane layer known in the art~
Among the patents and publications which describe ion~selective membranes of the type useful in the instant invention are:
U.s. Patent 3,562,129 to Simon, issued ~ebruary 9, 1971;
U.S. Patent 3,753,887 to Kedem et al, issued August 21 1973;
U.S. Patent 3,856,649 to Genshaw et al, issued December 24, 1974;

British Patent 1~375,446, issued November 27, 1974, German OLS 2,251,287, issued April 26, 1973;
~ . E. Morf, G. Kohr and W. Simon, "Reduction of the Anion Interference in Neutral Carrier Liquid-Membrane Electrodes Responsive to Cations," AnalYtical Letters, Volume 7, No. 1, pages 9 through 22 (197~
W. E. Morf, D. Ammann, E. Pretsch and W. Simon, "Carrier ~ntibiotics and Model Compounds as Components of Ion-Sensitive Electrodes", Pure and Applied Chemistry, Volume 36, No. 4, pages 421 through 439 (1973);
D. Ammann, E. Pretsch and W. Simon, "Sodium Ion-Selective Electrode Based on a Neutral Carrieri', Ana]ytical Letters, Volume 7, No. 1, pages 23 through 32 (1974)j R. W. Cattrall and H. Freiser, Analytical Chemistry, _, 1905 (1971); and H. James, G. Carmack and H. Freiser, Ana1ytical Chemistry, 44, 856 (1972).
_ Membranes of this type are well known. Such membranes generally include an inert hydrophobic binder or matrix having dispersed therein an ion carrier or selector commonly referred to as an ionophore which imparts selectivity to the membrane. m ese membranes can also contain a carrier solvent for the ionophore to provide adequate ion mobility in the membrane. The carrier solvent generally also serves as a plasticizer ~or the membrane binder.
The membrane layer generally contains binders, ion carriers, solvents and the like, such as described in U.S. Patent 4,214,968 of Battaglia et al, issued July 29, 1980.
The ion-selective electrodes can be manufactured using a conductive wire as the starting material and dipping the wire sequentially into the reference composition and the composition containing the membrane; or a dry operative electrode can be prepared by coating, laminating or other-wise applying the individual layers one over another to form a planar, multilayer electrode structure.
Thus, as seen in Figure 1, a typical manu~acturing procedure for a metal-insoluble metal salt-electrol~te 3'~
_ g _ reference element would lnvolve applying the re~erence composi~ion 3 to a layer of sllver 2 Yacuum-deposited on a poly(ethylene terephthalate) support 1~ drying, ovcrcoatlng the reference element with a solutlon 4 of the components of the ion-selective membrane and drying to provlde a complete electrode. Alternatively, the layers can be laminated, so long as intimate contact between layers ls achleved and maintained, and uniformity of thickness of the lon-selective membrane is attained. A potential ls set up u~lng the probe 5 connected to the silver layer.
The particular drying conditions which must be applied to the reference element in the manu~acture of any speci~ic ion-selective electrode will, of course, vary greatly, depending upon the composition of the electrode layers, particularly the binder used, the solvent or dls-persing medium used to rorm the layer, and these can be readily determined by the skilled artisan. Typical such conditions are described in the examples below for layers of the composition described therein.
The ion selectivity Or membrane electrodes can be observed by measuring a steady-state di~ference ln elec-trical potential between reference solutions and ~ample solutions, as described in U.S. Patent 4,214,968 of Battaglia et al, issued July 29, 1980.
The following examples will ~erve to better demon-strate the success~ul practice of the present lnvention.

Example 1 A ~ilver-coated poly(ethylene terephthalate) support was ~tripe coated ~lth an electrolyte layer -con~
taining, in 5 g/m2 of gelatin, chloride ~alts ~nd oxldi~ing agents, as shown ln Table I. A carbonate-~ensltive membrane containing 6 g/m2 polyt~inyl chlorlde~ tVYNS~ (Unlon Car-bide)), 4 g/m poly~vinyl acetate~ (VAG~ nion Carbide))~
5 g/m2 4-dec~ltrifluoroacetophenoneg 10 g/m2 diisodecyl-phthalate, 1.25 gjm2 trloctylpropylammonium chloride and0.05 g/m2 surfactant c~ntaining a mlxture Or p~lydlme~hyl-phenyl silo~ane and polymethylphe~yl ~llo~ane ~Do~ Corning 510 fluid) was coated over ~he reference elemen~ ln ~he . ~ . , .

areas coated with the electrolyte layer. A control rerer-ence element was prepared according to the methods of the prior art by overall coating the silver-coated support with an oxidizing agent to form a silver layer. The reference electrode was then subsequently overcoated in striped fashion with an electrolyte layer containing gelatin and chloride salts. The membrane layer was stripe coated over the electrolyte layer. The electrodes were tested ln the absolute mode using an Ag/AgCl reference electrode and saline samples containing 10, 40 and 100 mM HC03 buffered with tris-HCl (pH 8.25~ 0.062-0.576 mM carbonate activi-ties).
The results shown in Table I indicate that the electrodes prepared by the method of the present invention exhibit stable potential-time profiles that are comparable to the control. The figure Sy.x ~ndicated how well the data points fit on a straight line when plotted. Low Sy.x flgures indicate the reproducibility of the data is excellent.

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Example 2 Reference elements prepared as shown in Table II
were overcoated with carbonate-sensitive membranes as in Example 1.

TABLE II

Example NaCl KClCrO3 K3Fe(CN)6 Base Control - - - AgCl 2A 2.4 0.1 - Ag 2B 2.1 - 0.4 Ag 10 The electrodes were tested in a differential mode using a buffered C02 calibrator containing 30.22 mM C02 as the reference solution and one containing 59.67 mM C02 as the sample solution. The results shown in Table III indi-cate that incorporated oxidizing agents surprisingly do not adversely affect the electrode behavior, i.e., the potentials obtained from the electrodes coated by the method of the present invention are similar to those of the control elec-trode. This is unexpected, as the electrodes are generally sensitive to interference and one would expect the addition of oxidizing agents to interfere. Consistently good contact was made to the silver layer in the examples (as evidenced by the low rate of re~ections of Example 2B).

TABLE II_ Total Number Percent Example Potential SD of Runs Control -5.1 mV o.8 mV 23 l~
2A -5.8 mV 2.0 mV 22 8 2B -4.7 mV 0.9 mV 24 0 Example 3 A silver-coated polyethylene terephthalate support was coated in striped fashion with an electrolyte layer containing in 5 g~m of gel: a photographic-type, silver-chloride emulsion (10 g/m2), KN03 (0.83 g/m2) (optional) andoctylphenoxy polyethoxyethanol (Triton~ X-100, Rohm and Haas Company) (0.2 g/m2). A control reference element was pre-pared according to the method of the control element of Example 1. The reference elements were overcoated with carbonate-sensitive membrane layers as above and evaluated using absolute measurements aga~nst a Ag/AgCl reference electrode. Tris-HCl buffered calibrator solutions con-taining 1~ 4, lO, 40 and lO0 mM concentrations of HC03 were used in testing the electrodes (pH 8.25, 0.006-0.576 mM
carbonate activities). As can be seen in Table IV, the emulsion-containing electrode responded similarly to the control electrode. Therefore, this method of the present invention simplified manufacture of the reference element with adequate performance and provided good contact through the uncoated silver layer.

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The invention has been described in detail with particular reference to preferred embodiments thereof~ but it will be understoo~ that variations and modlflcations can be effected within the spirit and scope of the invention.

. ' ' :
.

Claims

I CLAIM:

1. A process of preparing a metal/silver halide reference element comprising coating portions of a metal layer with a metal salt electrolyte, a hydrophilic binder and a member of the group con-sisting of an oxidizing agent and a silver halide emulsion.

2. The process of claim 1 wherein the coating contains a silver oxidizing agent and the metal is silver.

3. The process of claim 2 wherein the silver oxidizing agent is selected from the group consisting of KClCrO3, K3Fe(CN)6, KMnO4, K2Cr2O7, NH4VO3, (NH4)2Ce(NO3)6 and Fe2(C2O4)3.

4. The process of claim 1 wherein the metal layer is coated on a support.

5. The process of claim 1 wherein the coating contains a silver halide emulsion.

6. The process of claim 1 wherein the metal salt electrolyte is selected from the group consisting of halides of ammonium, alkali metal and alkaline earth metal.

7. A metal/silver halide reference element comprising a support having thereover a layer of metal, portions of said layer of metal having thereon a composition comprising a metal salt electrolyte, a hydrophilic binder and a member of the group con-sisting of an oxidizing agent and a silver halide emulsion; portions of said layer of metal being free from said composition.

8. The element of claim 7 wherein the metal layer is coated with said composition in stripes

9. The element of claim 7 wherein the com-position contains a silver oxidizing agent and the metal is silver.

10. The element of claim 9 wherein the silver oxidizing agent is selected from the group con-sisting of KClCrO3, K3Fe(CN)6, KMnO4, K2Cr2O7, NH4VO3, (NH4)2Ce(NO3)6 and Fe2(C2O4)3.

11. The element of claim 7 wherein the metal salt electrolyte is selected from the group consisting of halides of ammonium, alkali metal and alkaline earth metal.

12. An ion-selective electrode comprising a support having thereon a silver layer, portions of said silver layer having thereon a first reference composition comprising a metal salt electrolyte, a hydrophilic binder and an oxidizing agent; portions of said silver layer being free from said composition;
and, said first reference composition having thereon a membrane layer comprising an ionophore, an ion-carrier solvent and a hydrophobic binder.

13. The electrode of claim 12 wherein the silver oxidizing agent is selected from the group con-sisting of KClCrO3, K3Fe(CN)6, KMnO4, K2Cr2O7, NH4VO3, (NH4)2Ce(NO3)6 and Fe2(C2O4)3.

14. The electrode of claim 12 wherein the silver layer is coated with said composition in stripes.

15. The electrode of claim 12 wherein the reference composition also contains a surfactant.

16. The electrode of claim 12 wherein the membrane layer also contains a surfactant.

17. An ion-selective electrode comprising a support having thereover a layer of silver, portions of said layer of silver having thereon a composition comprising a metal salt electrolyte, a hydrophilic binder and an oxidizing agent; portions of said silver layer being free from said composition; and, said com-position having thereover a polymeric layer.

18. An ion-selective electrode comprising a support having thereon a metal layer, portions of said metal layer having thereon a first reference composi-tion comprising a metal salt electrolyte, a hydro-philic binder and a silver halide emulsion; portions of said metal layer being free from said composition;
and, said first reference composition having thereon a membrane layer comprising an ionophore; an ion-carrier solvent and a hydrophobic binder.

19. The electrode of claim 18 wherein the metal layer is coated with said composition in a striped manner.

20. The electrode of claim 12 wherein the reference coating composition also contains a surface-ant.

21. The electrode of claim 18 wherein the membrane layer also contains a surfactant.

22. An ion-selective electrode comprising a support having thereover a layer of metal, portions of said layer of metal having thereon a composition com-prising a metal salt electrolyte, a hydrophilic binder and a silver halide emulsion; portions of said metal layer being free from said composition; and, said com-position having thereover a polymeric layer.