CA2027536A1 - Method for determination of e.coli in water - Google Patents

Method for determination of e.coli in water

Info

Publication number
CA2027536A1
CA2027536A1 CA2027536A CA2027536A CA2027536A1 CA 2027536 A1 CA2027536 A1 CA 2027536A1 CA 2027536 A CA2027536 A CA 2027536A CA 2027536 A CA2027536 A CA 2027536A CA 2027536 A1 CA2027536 A1 CA 2027536A1
Authority
CA
Canada
Prior art keywords
coli
detection
media
water
determination
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2027536A
Other languages
French (fr)
Other versions
CA2027536C (en
Inventor
George Chang
Rosalind Lum
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2027536A1 publication Critical patent/CA2027536A1/en
Application granted granted Critical
Publication of CA2027536C publication Critical patent/CA2027536C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/045—Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/10—Enterobacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/24—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • G01N2333/245—Escherichia (G)

Abstract

A novel highly sensitive method for detection of the presence of total coliform and E. coli in water samples, food products, and seafood employs a new type of media having composition which allows an unequivocal detection of very small number of E. coli. The new low pH media which contain trimethylamine-N-oxide promote the growth of chlorine or food processing injured E. coli for their easier detection.
CA002027536A 1990-05-14 1990-11-06 Method for determination of e.coli in water Expired - Lifetime CA2027536C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US52332090A 1990-05-14 1990-05-14
US523,320 1990-05-14

Publications (2)

Publication Number Publication Date
CA2027536A1 true CA2027536A1 (en) 1990-11-06
CA2027536C CA2027536C (en) 1993-02-16

Family

ID=24084534

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002027536A Expired - Lifetime CA2027536C (en) 1990-05-14 1990-11-06 Method for determination of e.coli in water

Country Status (10)

Country Link
US (1) US5643743A (en)
EP (1) EP0530322B1 (en)
JP (1) JP3034036B2 (en)
AT (1) ATE143416T1 (en)
AU (1) AU657688B2 (en)
CA (1) CA2027536C (en)
DE (1) DE69122385T2 (en)
FI (1) FI925169A0 (en)
HU (1) HU9203390D0 (en)
WO (1) WO1991018111A1 (en)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2204121C (en) * 1994-11-04 2010-12-21 Elizabeth Ehrenfeld Medium for detecting target microbes in a sample
US5610029A (en) * 1994-11-04 1997-03-11 Idexx Laboratories, Inc. Medium for detecting target microbes in a sample
US6107462A (en) 1995-08-17 2000-08-22 The Regents Of The University Of California Genes and proteins controlling cholesterol synthesis
FR2770538B1 (en) 1997-11-06 2000-10-13 Bio Merieux METHOD AND AGENT FOR DETECTION AND IDENTIFICATION AND / OR QUANTIFICATION OF DESAMINASE-LIKE ENZYMATIC ACTIVITY
US5935799A (en) * 1997-12-10 1999-08-10 George Mason University Biological assay for microbial contamination
US5968762A (en) * 1998-03-19 1999-10-19 The University Of Connecticut Method for detecting bacteria in a sample
US7344854B2 (en) * 1999-07-20 2008-03-18 Micrology Laboratories, Llc Test media for quantitative or qualitative identification and differentiation of general coliforms, E. coli, Aeromonas spp and Salmonella spp in a test sample
US6350588B1 (en) * 1999-07-20 2002-02-26 Micrology Laboratories, Llc Test media and quantitative or qualitative method for identification and differentiation of biological materials in a test sample
US7273719B2 (en) * 1999-07-20 2007-09-25 Micrology Laboratories, Llc Test media for quantitative or qualitative identification and differentiation of general coliforms, E coli, Aeromonas spp and Salmonella spp materials in a test sample
US6482642B2 (en) 2001-03-29 2002-11-19 Environmental Biodetection Products.Com Testing kit and methodology for testing for the presence of microorganisms
US6440149B1 (en) 2001-04-23 2002-08-27 Dasan Potti Tongue and tooth cleaning device
US7026111B2 (en) * 2001-10-15 2006-04-11 Beckman Coulter, Inc. Methods and reagents for improved cell-based assays
FR2881755B1 (en) 2005-02-10 2012-11-30 Biomerieux Sa MEDIA FOR THE SPECIFIC DETECTION OF RESISTANT MICROORGANISMS
FR2882370B1 (en) * 2005-02-22 2010-12-03 Alain Rambach DETECTION OF A MICROORGANISM STRAIN IN A LIQUID SAMPLE
US20060223052A1 (en) * 2005-03-30 2006-10-05 Kimberly-Clark Worldwide, Inc. Technique for detecting microorganisms
US20070281291A1 (en) * 2006-05-22 2007-12-06 Kuchta John M Method and Medium for the Rapid Detection of E.Coli in Liquid Samples
FR2912425B1 (en) * 2007-02-08 2012-08-31 Biomerieux Sa MEDIUM FOR DETECTION AND / OR IDENTIFICATION OF BACTERIA
US9376704B2 (en) 2007-02-12 2016-06-28 Charm Sciences, Inc. Test kit and method for detecting bacteriophage
EP2350306A4 (en) * 2008-10-20 2012-05-02 Photonic Biosystems Inc Integrated bioanalyzer
CN101914609B (en) * 2010-08-27 2012-05-23 殷涌光 Rapid detection method of coliform groups
KR101343353B1 (en) 2012-05-04 2013-12-20 한국과학기술연구원 Method for differentiating from Enterobacter and Escherichia
WO2019033086A1 (en) * 2017-08-11 2019-02-14 Tab Health Solutions Human waste detecting and signaling devices, methods, and systems
WO2019157063A1 (en) * 2018-02-07 2019-08-15 White Dog Labs, Inc. An improved method for selecting microorganisms
CN109576148A (en) * 2018-12-26 2019-04-05 中国航天员科研训练中心 A kind of coliform detection device and its detection method
CN111304280A (en) * 2020-02-26 2020-06-19 成都海关技术中心 Culture medium for color development detection of coliform group in food and seasoning containing pepper

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3870601A (en) * 1973-05-04 1975-03-11 Schering Corp Novel diagnostic system for differentiation of enterobacteriaceae
US4245043A (en) * 1979-06-29 1981-01-13 Minnesota Mining And Manufacturing Company Negative control media device and method for microbiologic biochemical tests
JPS5817598B2 (en) * 1979-10-31 1983-04-08 味の素株式会社 Rapid detection method for microorganisms
US4925789A (en) * 1986-06-30 1990-05-15 Edberg Stephen C Method and medium for use in detecting target microbes in situ in a specimen sample of a possibly contaminated material
CA1317534C (en) * 1987-03-17 1993-05-11 Arthur Newton Ley Method of enumerating escherichia coli
US5411867A (en) * 1990-05-14 1995-05-02 The Regents Of The University Of California Method for determination of E. coli in water

Also Published As

Publication number Publication date
JPH05508992A (en) 1993-12-16
AU8077191A (en) 1991-12-10
WO1991018111A1 (en) 1991-11-28
EP0530322B1 (en) 1996-09-25
EP0530322A4 (en) 1994-06-29
AU657688B2 (en) 1995-03-23
US5643743A (en) 1997-07-01
DE69122385D1 (en) 1996-10-31
FI925169A (en) 1992-11-13
DE69122385T2 (en) 1997-04-10
ATE143416T1 (en) 1996-10-15
JP3034036B2 (en) 2000-04-17
CA2027536C (en) 1993-02-16
HU9203390D0 (en) 1993-04-28
EP0530322A1 (en) 1993-03-10
FI925169A0 (en) 1992-11-13

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