CA2038398C - Dna encoding a growth factor specific for epithelial cells - Google Patents

Abstract

Discoveries are disclosed that show particular aspects of recombinant DNA technology can be used successfully to produce hitherto unknown human keratinocyte growth factor (KGF) protein free of other polypeptides. These proteins can be produced in various functional forms from spontaneously secreting cells or from DNA segments introduced into cells. These forms variously enable biochemical and functional studies of this novel protein as well as production of antibodies. Means are described for determining the level of expression of genes for the KGF protein, for example, by measuring mRNA levels in cells or by measuring antigen secreted in extracellular or body fluids.

Description

2~~~~~.~
FIELD OF THE INVENTION
The present invention relates to growth factors, particularly to isolation of a polypeptide growth factor similar to a family of factors including known fibroblast growth factors (FGFs). This invention also relates to construction of complementary DNA
(cDNA) segments from messenger RNA (mRNA) encoding the novel growth factor. Further, this invention pertains to synthesis of products of such DNA segments by recombinant cells, and to the manufacture and use of certain other novel products enabled by the identification and cloning of DNAs encoding this growth factor. In addition, a high affinity receptor is provided for the novel growth factor.

2~~~~~8 ABBREVIATIONS USED
IN THIS APPLICATION

aFGF acidic fibroblast growth factor bFGF basic fibroblast growth factor EGF epidermal growth factor HSAC heparin-Sepharose affinity chromatography kb kilobases Kd dissociation constant kDa kilodaltons KGF keratonicyte growth factor NaDodSo4/PAGE Sodium dodecylsulfate (SDS)/polyacrylamide gel electrophoresis RP-HPLC reversed-phase high performance TGFa transforming growth factor a ~C1CGRO~IdD OF THE INVEIdTIOI~I~ ~ ~ f~ ~
Growth factors are important mediators of intercellular communication. These potent molecules are generally released by one cell type and act to influence proliferation of other cell types (sea reference I-1 in Experimental Section I, below). Interest in growth factors has been .. heightened by evidence of their potential involvement in neoplasia (reference II-2 in Experimental Section II, below). The v-sis transfonaing gene of simian sarcoma virus encodes a protein that is homologous to the 8 chain of platelet-derived growth factor (I-1, I-2).
Moreover, a number of oncogenes are homologues of genes encoding growth factor receptors (I-1).
Thus, increased understanding of growth factors and their receptor-mediated signal transduction pathways is likely to provide insights into mechanisms of both normal and malignant cell growth.
One known family of growth factors affecting connective tissue cells includes acidic fibroblast growth factor (aFGF), basic fibroblast growth factor (bFBF), and the related products of 2 5 the hst and int-1 oncogenes .
Further, it is known that some growth factors, including the following, have heparin-2~a s3~~
binding properties: aFGF (I-20, I-21): bFGF (I-19, I-20): granulocyte/macrophage colony stimulating factor (I-1): and interleukin 3 (I-1). Each of these polypeptide factors is produced by stromal cells (I-1, I-2, I-25). Such factors appear to be deposited in the extracellular matrix, or on proteoglycans coating the stromal cell surface (I-1, I-25). It has been postulated that their storage, release and contact with specific target cells are regulated by this interaction (I-25, I-28).
It is widely recognized, however, that the vast majority of human malignancies are derived from epithelial tissues (I-5). Effectors of epithelial cell proliferation derived from mesenchymal tissues have been described (I-1, I-2, I-3), however, their molecular identities and structures have not been elucidated.
In light of this dearth o! knowledge about such mesenchymal growth factors affecting epithelial cells, it is apparent that there has been a need for methods and compositions and bioassays which would provide an improved knowledge and analysis of mechanisms of regulation of epithelial cell proliferation, and, ultimately, a need for novel diagnostics and therapies based on the factors involved therein.

2~~$3~~
This invention contemplates the -~ application of methods of protein isolation and recombinant DNA technologies to fulfill such needs and to develop means for producing protein factors of mesenchymal origin, which appear to be related to epithelial cell proliferation processes and which could not be produced otherwise. This invention also contemplates the application of the molecular mechanisms of these factors related to epithelial cell growth processes.

~~~~D~~
--The present invention relates to developments of protein isolation and recombinant DNA technologies, which include production of novel growth factor proteins affecting epithelial cells, free of other peptide factors. Novel DNA
segments and bioassay methods are also included.
The present invention in particular relates to a novel protein having structural and/or functional characteristics of a known - family of growth factors which includes acidic fibroblast growth factor (aFGF), basic fibroblast growth factor (bFBF) and the related products of the hst and int-1 oncogenes. This new member of the FGF polypeptide family retains the heparin-binding properties of tho FGF: but has ~volved a unique target cell specificity. This growth factor appears to be specific for epithelial cells and is particularly activa~on keratinocytes. Therefore, this novel factor has been designated "keratinocyte growth factor"
(KGF). Notwithstanding its lack of activity on libroblasts, since it is the seventh known member of the FGF polypeptide family, KGF may also be referred to as FGF-7.
Accordingly, this invention relates, in part, to purified RGF or KGF-like proteins and 2~~~~~~
methods for preparing these proteins. Such purified factors may be made by cultivation of human cells which naturally secrete these proteins and application of isolation methods according to the practice of this invention.
These proteins can be used for biochemical and biological studies leading, for example, to isolation of DNA segments encoding RGF or RGF-like polypeptides.
The present invention also relates to such DNA segments which encode KGF or RGF-like proteins. In a principal embodiment, the present invention relates to DNA segments, which encode RGF-related products, consisting o!: human cDNA
clones 32 or 49, derived from polyadenylated RNA
extracted from the human embryonic lung fibroblast cell line M426: recombinants and mutants of these clones: and related DNA segments which can be detected by hybridization to any of the above human DNA segments, which related segments encode RGF-like proteins or portions thereof.
In the practice of one embodiment o!
this invention, the DNA segments of the invention are capable of being expressed in suitable host cells, thereby producing KGF or RGF-like proteins. The invention also relates to mRNAs produced as the result of transcription of the sense strands of the DNA segments of this invention.
In another embodiment, the invention relates to a recombinant DNA molecule comprising a vector and a DNA of the present invention.
These recombinant molecules are exemplified by molecules comprising a RGF cDNA and any of the following vector DNAs: a bacteriophage a cloning vector (exemplified by apCEV9); a DNA sequencing plasmid vector (e. g., a pUC variant): a bacterial gene expression vector (e.g., pKIC233-2); or a mammalian gene expression vector (such as pI~I'r).
In still another embodiment, the invention comprises a cell, preferably a mammalian cell, transformed with a DNA of the invention. Further, the invention comprises cells, including insect cells, yeast cells and bacterial cells such as those of Bscherichia coli and B. subrilis, transformed with DNAs of the invention.
According to another embodiment of this aspect of the invention, the transforming DNA is capable of b.ing expressed in the cell, thereby increasing in the cell the amount of KGF or RGF-like protein encoded by this DNA.
The primary KGF translation product predicted from its cDNA sequence contains an N-terminal hydrophobic region which likely serves a as a signal sequence for secretion and which'%f3 ~ not present in the mature KGF molecule. In a most preferred embodiment of the gene expression aspect of the invention, the cell transformed by the DNA of the invention secretes the protein encoded by that DNA in the (truncated) form that is secreted by human embryonic lung fibroblast cells.
Still further, this invention contemplates KGF or~KGF-like proteins produced by expression of a DNA of the invention, or by translation of an RNA of the invention.
Preferably, these proteins will be of the secreted form (i.e., lacking an apparent signal sequence). These protein factors can be used for functional studies, and can be purified for additional structural and functional analyses, such as qualitative and quantitative receptor binding assays.
Moreover, the ability to produce large quantities of this novel growth factor by recombinant techniques will allow testing of its clinical applicability in situations where specific stimulation of growth of epithelial c~lls is of particular importance. Accordingly, this invention includes pharmaceutical compositions comprising KGF or RGF-like polypeptides for use in the treatment of such r conditions, including, for example, healing of wounds due to burns or stimulation of transplanted corneal tissue.
According to this embodiment of the invention, the novel KGF-like proteins will be protein products of "unmodified" DNAs and mRNAs o! the invention, or will bs modified or genetically engineered protein products. As a result of engineered mutations in the DNA
sequences, modified KGF-like proteins will have one or more differences in amino acid sequence from the corresponding naturally occurring "wild-type" proteins. According to one embodiment of this aspect of this invention, the modified RGF-like proteins will include "chimeric" molecules comprising segments of amino acid sequences of RGF and at least one other member of the FGF
peptide family.
Ultimately, given results of analogous successful approaches with other peptide factors having similar properties, development of such chimeric RGF-like polypeptides should lead to superior, "second generation" loans of KGF-like p~ptides for clinical purposes. These modified KGF-Like products might be smaller, more stable, more potent, and/or easi~r or less expensive to produce, for example.
l0 This invention further comprises novel bioassay methods for determining expression in human cells of the mRNAs and proteins produced from the genes related to DNA segments of the invention. According to one such embodiment, DNAs of this invention may be used as probes to determine steady state levels or kinetics of induction of related mRNAs. The availability of the RGF-related cDNA clones makes it possible to determine whether.abnonaal expression of this growth factor is involved in clinical conditions characterized by excessive epithelial cell growth, including dysplasia and neoplasia (e. g., psoriasis, or malignant or benign epithelial tumors).
This invention also contemplates novel antibodies made against a peptide encoded by a DNA segment of the invention. In this embodiment of the invention, the antibodies are monoclonal or polyclonal in origin, and are generated using KGF-related polypeptides from natural, .. recombinant or synthetic chemistry sources.
The antibodies of this invention bind specifically to KGF or a KGF-like protein which includes the sequence of such peptide, preferably when that protein is in its native (biologically active) conformation. These antibodies can be used for detection or purification of the RGF or il KGF-like protein factors. In a most preferred embodiment of this aspect of the invention, the antibodies will neutralize the growth promoting activity of KGF, thereby enabling mechanistic studies, and ultimately, therapy for clinical conditions involving excessive levels of KGF.
According to another aspect of the invention, a pharmaceutical composition is provided for treating conditions requiring specific stimulation of human keratinocytes or other epithelial cells while allowing for normal differentiation as evidenced by appearance of differentiation markers, such as Keratin 1 (K1) and/or filaggrin. The composition comprises KGF purified from a culture of recombinant transformed cells and an acceptable pharmaceutical carrier.
According to yet another aspect of the present invention, a substantially pure human keratinocyte growth factor (KGF) protein or a portion of the KGF protein having at least 9 consecutive amino acids of the KGF
protein, the KGF protein having an approximate molecular weight between 25 and 30 kDa, as determined by SDS-PAGE, and having preferential mitogenic activity for an epithelial cell.
According to another aspect of the present invention, a pharmaceutical composition comprises a protein or portion thereof as defined in the above aspects and a pharmaceutically acceptable carrier, for treating conditions requiring specific stimulation of epithelial cells.
According to another aspect of the present invention, a pharmaceutical composition comprises a protein or portion thereof as defined in the above aspects and a pharmaceutically acceptable carrier, for acceleration or improvement of wound healing involving the epidermis.
According to another aspect of the present invention, a chimeric protein which comprises within a single polypeptide molecule a functional domain of human zo3a~gs KGF protein, wherein the functional domain is defined by amino acids responsible for the preferential mitogenic activity of KGF for an epithelial cell, and at least one other portion polypeptide of the fibroblast growth factor family.
According to yet another aspect of the present invention, an antibody directed against KGF protein or a portion of KGF protein as defined in the above aspects.
According to yet another aspect of the present invention, a pharmaceutical composition comprises an antibody as defined in the above aspects and a pharmaceutically acceptable carrier, for treating conditions requiring specific inhibition of stimulation of epithelial cells by KGF protein.
According to another aspect of the present invention, a DNA molecule encoding a human keratinocyte growth factor (KGF) protein or a portion of said KGF
protein having at least 9 consecutive amino acids of said KGF protein, said KGF protein having an approximate molecular weight between 25 and 30 kDa, as determined by SDS-PAGE, and having preferential mitogenic activity for an epithelial cell.
According to another aspect of the present invention, a bioassay for mitogenic activity for a keratinocyte cell in a test sample which comprises the following steps: (i) growing keratinocytes in culture to confluence and maintaining the confluent culture in serum-free medium; (ii) adding a test sample to the confluent culture of keratinocytes; and (iii) determining the stimulation of DNA synthesis in the keratinocytes.
According to yet another aspect of the present invention, a method of producing KGF protein as defined in the above aspects comprises: (i) contacting a first solution comprising KGF protein with heparin under conditions such that the KGF protein binds to the heparin whereby a heparin-KGF complex is formed; (ii) separating the heparin-KGF complex from other components of the 12a first solution. And (iii) dissociating KGF protein from the heparin-KGF complex to form a second solution comprising KGF protein.
According to another aspect of the present invention, a keratinocyte growth factor defined by the amino acid sequence of Figure 11-1B, optionally lacking the N-terminal 31 amino acids, and proteins differing by the addition, deletion or substitution of one or more amino acids from the amino acid sequence of said factor, which retain the property of exhibiting at least about 500-fold greater stimulation of BALM/MK keratinocyte cells thanNIH/3T3 fibroblast cells, and at least about 50-fold greater stimulation of BALB/MK keratinocyte cells than of BS/589 epithelial cells and CCL208 epithelial cells, as determined by H-thymidine incorporation.
According to an aspect of the invention, a chimeric protein which comprises within a single polypeptide molecule a segment of KGF protein, wherein the segment of KGF protein is a segment of amino acids of Figure 11-1B which comprises a sufficient number of consecutive amino acids 32 - 189 of Figure II-1B to confer on the polypeptide molecule preferential mitogenic activity for an epithelial cell, and at least one other polypeptide portion of the fibroblast growth factor family.
According to a further aspect of the invention, An antibody specifically directed against glycosylated or unglycosylated keratinocyte growth factor (KGF) polypeptide selected from the group consisting of(a) the following amino acid sequence, (b) a portion of the following amino acid sequence without the N-terminal 31 12b amino acids, and (c) an amino acid sequence that differs by the addition, deletion, or substitution of one or more amino acids from the following amino acid sequence:
M H K W I L T W I L P T L L Y
R S C F H I I C L V G T I S L
A C N D M T P E Q M A T N V N
C S S P E R H T R S Y D Y M E
G G D I R V R R L F C R T Q W
Y L R I D K R G K V K G T Q E

G I V A I K G V E S E F Y L A
M N K E G K L Y A K K E C N E
D C N F K E L I L E N H Y N T
Y A S A K W T H N G G E M F V
A L N Q K G I P V R G K K T K
K E Q K T A H F L P M A I T

12c BRIEF DESCRIPTION OF T8E DRAi~INGB
Fig. I-1 depicts results of heparin-Sepharose affinity chromatography of conditioned medium from M426 human embryonic fibroblasts showing that greater than 90~ of the mitogenic activity for mouse keratinocytes (BALB/MK) eluted with 0.6 M NaCl.
Fig. I-2 illustrates results of further purification of the mitogen from human fibroblasts using HPLC with and adsorptive matrix. Panel (A) shows the profile on reversed-phase (C~) HPLC of BALB/MK mitogenic activity.
Panel (B) presents electrophoretic (NaDodSO~/PAGE) analysis of selected fractions from the C~
chromatography shown in panel A, demonstrating that the peak HPLC fractions contained a single band on the silver stained gel. Panel (C) is a bar graph of DNA synthesis in BALB/MK cells triggered by the fractions analyzed in Panel B, showing that the relative mitogenic activity correlated well with the intensity of the protein band across the activity profile.
Fig. Z-3 presents an alternative purification step to RP-HPLC, using sieving chromatography with a (TSK G3000SW GlasPac)*
column run in aqueous solution near physiologic * trade mark pH, which resulted in a major peak of mitoge~i.G
activity in the BALB/MK bioassay.
Fig. I-4 illustrates a comparison of BALB/MK DNA synthesis in response to TSK-purified mitogen and other growth factors.
Fig. I-5 shows comparisons~of growth of BALB/MK cells in a chemically defined medium in response to different combinations of growth factors.
Table I-1 summarizes the results from various purification steps, documenting that sieving chromatography provided a far better recovery of activity than the adsorptive RP-HPLC
...
approach.
Table I-2 recapitulates data on the target cell specificities of various growth factors, demonstrating that the newly isolated factor exhibited a strong mitogenic effect on keratinocytes (BALB/MK) and, in striking contrast, had no detectable effects on fibroblasts or human saphenous vein endothelial cells.
Fig. II-1 presents the nucleotide sequence and deduced amino acid sequence of KGF
cDNA, as well as identification of RNAs transcribed from the KGF gene. Panel (A) outlines a schematic representation of human KGF
cDNA clones. Panel (H) documents the KGF cDNA

nucleotide and predicted amino acid sequences. (C) Identification of RNA transcripts of KGF genes by Northern blot analysis. (D) cDNA sequence and deduced amino acid sequence for aFGF.
Fig. II-2 illustrates the topological comparison of the FGF family of related molecules, including KGF, with emphasis on the two protein domains that share high homology, the putative signal peptide sequences, and the two conserved cysteine residues.
Fig. II-3 shows (Northern blot) analyses of expression of KGF-related mRNA in selected normal human cell lines and tissues, revealing that a single 2.4 kb transcript was present in RNA from human embryonic lung fibroblasts and from adult skin fibroblasts, while no transcript was detected in the (B5/589) epithelial or (HA83) glial cell lines, or in primary cultures of human saphenous vein endothelial cells.
Fig. II-4A represents the Mono-S-Chromatography*pattern of heparin-Sepharose purified non-glycosylated KGF.
Fig. II-4B shows the SDS-PAGE analysis of mitogenically active fractions from KGF preparation.
Fig. II-4C shows the immunoblot analysis of selected fractions from the Mono-S-Chromatography.
* trade mark B

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Fig. III-1 are phase contrast micrographs A, B and C of human epidermal keratinocytes grown in low Ca2+ .
Fig. III-2 is a dose response profile of KGF
(solid dot) and EGF (open circle) on proliferation of cultured human epidermal keratinocytes.
Fig. III-3 is a time course of KGF and EGF-induced proliferation of human epidermal keratinocytes.
Fig. III-4 shows the effect of Caz+
concentration on growth factor-induced proliferation of human epidermal keratinocytes.
Fig. III-5 are phase contrast micrographs A, B and C of human epidermal keratinocytes grown in high Ca2+ concentration.
Fig. III-6 is an immunoblot analysis of keratinocyte differentiation markers expressed in response to different Ca2+ concentrations and growth factors.
Fig. III-7 is a comparison of effects of TGFa, EGF and KGF on keratin-1 expression by keratinocytes at low and high Ca2+ concentration.
Fig. IV-1 illustrates (A) ligand competition of '~sI-KGF specific binding to BALB/MK cells, (B) ligand competition of l~sI-KGF specific binding to NIH/3T3 cells, (C) ligand competition of 'ZSI-aKGF
specific binding to BALB/MK cells and (D) ligand ~-- L ~ '~ \-i ~~~~?vt~~
competition of lzsl-FGF specif is binding of lxsI-FGF to NIH/3T3 cells.
Fig. IV-2. (A) is a Scatchard analysis of ixsl-KGF and ~xsI-aFGF specif is binding to BALB/MK cells and (B) is a Scatchard analysis of lzsl-KGF binding on BALB/MK cells in presence or absence of heparin.
Fig. IV-3 shows the covalent affinity cross-linking of lxsl-KGF, lxsI-aFGF and lxsI-bFGF to intact BALB/MK and NIH/3T3 cells.
l0 Fig. IV-4 is an autoradiogram of phosphotyrosyl proteins from intact Balb/MK and NIH/3T3 cells following treatment with KGF, aFGF or bFGF.
Fig. V-1 is (A) Southern blot analysis of SAL1-digested genomic DNA from transfectant and untransfectant NIH/3T3 cells and (B) Southern analysis of Eco RI-digested DNAs of different animal species and (C) Northern analysis of NIH/3T3 and BALB/MK RNA.
Fig. V-2 (A) is primary aminoacid structure of KGF receptor and (B) structural comparison of predicted KGF and bFGF receptors.
Fig. V-3 is competition of KGF, aFGF and bFGF
for lxsl-labelled KGF binding on (A) BALB/MK cells and (B) NIH/ecti cells (o) -KGF, (O) -aFGF, (o ) bFGF.
Fig. V-4 is an analysis of KGF receptor, expressed in NIH/3T3 cells. Panel A is covalent affinity cross-linking of lxsl_KGF to BALB/MK, NIH/3T3 15b 2~~~~~~
and NIH/ecti cultures and panel B is an autoradiogram of phosphotyrosyl-proteins from intact NIH/3T3 and NIH/ecti cells.
Table II-1 summarizes a comparison of the effect of heparin on KGF mitogenic activity with effects on other growth factors, showing that thymidine incorporation into DNA by BALB/MK cells in response to KGF was inhibited by heprain, in contrast, to the activities of both aFGF and bFGF which were increaed by the same treatment.
c Y~'a~gIPTION OF SPECIF ' 3 This invention relates, in part, to purified KGF or KGF-like proteins and methods for preparing these proteins. A principal embodiment of this aspect of this invention relates to homogeneous KGF characterized by an apparent molecular weight of about 28 kDa based on .. migration in NaDodSO~/PAGE, movement as a single peak on reversed-phase high. performance liquid chromatography, and a specific activity of at least about 3.4 x 10~ units per milligram, and preferably at least about 3.2 x 10s units,per milligram, where one unit of activity is defined as that amount which causes half of the maximal possible stimulation of DNA synthesis in certain epithelial (keratinocyte) calls under standard assay conditions outlined below.
To identify novel growth factors specific for epithelial cell types, a clonal BALB/c mouse keratinocyte cell line, designated gj,,I,g/~ (I-6) was employed as an indicator cell to detect such factors. These cells are dependent !or their growth upon an exogenous source of an epithelial cell mitogan even in medium containing serum (I-6). The d~velopment of chemically defined medium for these cells has made it possible to demonstrate that two major ib mitogenic pathways are required for BALB/MK
..... proliferation. One involves insulin-like growth factor I (or insulin at high concentration) and the other is satisfied by epidermal growth factor (EGF), transforming growth factor a (TGFa), acidic fibroblast growth factor (aFGF) or basic fibroblast growth factor (bFBF) (I-7).
By using HALB/MK as the prototypical - , epithelial cell line and NIH/3T3 as its fibroblast counterpart, conditioned media from various human cell lines were assayed for new epithelial cell-specific mitogens. These bioassays of this invention enabled the purification to homogeneity of one such novel growth factor, released by a human embryonic lung fibroblast line, and designated herein as keratinocyte growth factor (RGF).
In brief, the bioassay for KGF-like activity under standard conditions comprises the following steps:
(i) Mouse keratinocytes (HALB/MR cells) are grown in culture to confluency and then maintained for 24-72 hr fn serum-free medium;
(ii) Following addition of test samples, stimulation of DNA synthesis is determined by incorporation of ~-thymidine into acid precipitable DNA.

To determine the cell target specificity of a mitogenic growth factor, the DNA synthesis stimulation, expressed as ratio of stimulated synthesis over background incorporation of thymidine in the absence of added test sample, can be compared to analogous stimulation observed in cells other than keratinocytes under the same assay conditions. In such comparisons, RGF
mitogenic activity will exhibit marked specificity for the keratinocytes as opposed to fibroblasts (at least about 500-fold greater stimulation) and lesser but significant (at least about 50-fold) greater activity on keratinocytes than on other exemplary epithelial call types (see Table I-2 for further data, and Materials and Methods in Experimental Section I for details of the standard conditions of the bioassay).
By employing a method of RGF production involving culturing cells and isolating mitogenic 2o activity, which method comprises ultrafiltration, heparin-Sepharost affinity chromatography (HSAC) and adsorptiv. reversed-phase high performance liquid chromatography (RP-HPLC) or, alternatively, molecular sieving HPLC (TSR-HPLC), according to th~ present invention, a quantity was isolated sufficient to per~it detailed characterization of the physical and biological properties of this molecule.

To summarize, the method for production of KGF from producing cells such as M426 human embryonic fibroblasts (I-8), for example, comprises the following steps:
(i) Preparation of conditioned media (e. g., liters) using monolayer cultures cycled from serum-containing to serum-free medium and storing the serum-free harvest at -70'C until further use:

10 (ii) Concentration by ultrafiltration using membranes having a 10 kDa molecular weight cutoff in several successive steps with intervening dilution in butter (to facilitate removal of low molecular weight materials), followed by optional storage at -70'C:
(iii) Affinity chromatography on heparin attached to a polymeric support (s. g., Sepharose) with elution by a gradient of increasing NaCl concentration:
(iv) Concentration by a factor of at least ten- to twenty-fold with small scalp ultrafiltration devices with a 10 kDa molecular weight cutoff (e.g., a Centricon-10 aicroconcentrator from A:eicon) and storage at -70'C.
Tha next step of the purification process comprises either step (v) or, alternatively, step (vi), as follows:

(v) Reversed-phase HPLC of active fractions ,,..... (0.6 M NaCl pool) from the previous HSAC step i~
organic solvent systems:
or, (vi) Molecular sieve HPLC (e. g, on a TSK-G3000SW Glas-Pac Column from LRB) in aqueous buffer at near physiological p8 (..g., Tris-HC1, pH 6.8/0.5M NaCl) followed by storage at -70~C.
A preparation made by the TSR step (vi) was almost as pure as one obtained from RP-HPLC, as judged by silver-stained NaDodSO,~/PAGE (data not shown); but the TSR approach provided a far better recovery of activity (Table I-1).
Further, the TSR-purified material had a higher specific activity than the RP-BPLC material. RGF
prepared by the TSR procedure above stimulated DNA synthesis in epithelial cells at sub-nanomolar concentrations, but failed to induce any thymidina incorporation into DNA of fibroblasts or endothelial cells at comparable or higher concentrations (up to 5 nM). The activity was sensitive to acid, heat and solvents used in the RP-HPLC step. (Ses Experimental Section I for data on sensitivities and further details of the production method.) Using standard methodology well known in the art, an unambiguous amino acid aequence was determined for position: 2-13 from the amino terminus of the purified KGF, as follows: Asn s ~'~°
Asp-Met-Thr-Pro-Glu-Gln-Met-Ala-Thr-Asn-Val (see Experimental Section I).
The present invention also includes DNA
segments encoding RGF and KGF-like polypeptides.
The DNAs of this invention are exemplified by DNAs referred to herein as: human cDNA clones 32 and 49 derived from polyadenylated RNA extracted from the human embryonic lung fibroblast cell line M426: recombinants and mutants of these clones: and related DNA segments which can be detected by hybridization to these DNA segments.
As described in Experimental Section II, to search for cDNA clones corresponding to the known portion of the RGF amino acid sequence, two pools o! oligonucleotide probes were generated based upon all possible nucleotide sequences encoding the nine-amino acid sequence, Asn-Asp-Met-Thr-Pro-Glu-Gln-Met-Ala. A cDNA
library was constructed in a cDN~ cloning vector, apCEV9, using polyadenylated RNA extracted from - the human embryonic lung fibroblast cell line M426 which was th. initial source of the growth !actor. Screening of the library (9 x lOs plaques) with th. ~P-labelled oligonucleotides identified 88 plaques which hybridized to both probe:.

Of 10 plaque-purified clones that were 2~~~~~~
analyzed, one, designated clone 49, had a cDNA
insert of 3.5 kb, while the rest had inserts ranging from 1.8 kb to 2.1 kb. Analysis of the smaller clones revealed several common restriction sites, and sequencing of a representative smaller clone, designated clone 32, along with clone 49, demonstrated that they were overlapping cDNAs (Fig II-lA). Alignment of the two cDNAs established a continuous sequence of 3.85 kb containing the complete KGF coding sequence. The sense strand DNA nucleotide sequence, and the predicted primary protein sequence encoded, are shown for the full-length composite RGF cDNA sequence in Fig. II-18.
These DNAs, cDNA clones 32 and 49, as well as recombinant forms of these segments comprising the complete KGF coding sequence, are most preferred DNAs of this invention.
From the cDNA sequence, it is apparent that the primary KGF and hst translation products contain hydrophobic N-terminal regions which likely serve as signal sequences, based on similarity to such sequences in a variety of other proteins. Accordingly, this N-terminal domain is not present in the purified matur. KGF
molecule which is secreted by human embryonic fibroblasts.

2~3~~~~
Furthermore, KGF shares with all other members of the FGF family two major regions of homology, spanning amino acids 65-156 and 162 189 in the predicted KGF sequence, which are separated by short, non-homologous series of amino acids of various lengths in the different family members. The non-homologous sections may or may not play a role in determining the unique functional aspects of KGF.
Furthermore, such non-homologous sections may also be unique in distinguishing from other known sequence portions of other growth factors.
The sequence of the purified form of KGF
contains five cysteine residues, two of which are conserved throughout the family of FGF related proteins. Five pairs of basic residues occur throughout the KGF sequence. This same pattern has been observed in other FGF family members.
It should be obvious to one skilled in the art that, by using the DNAs and RNAs of this invention in hybridization methods (such as Southern blot analyses of genomic human DNAs), especially the most preferred DNAs listed herein above, without undue experimentation, it is possible to screen genomic or cDNA libraries to find other KGF-like DNAs or variants which fall within the scope of this invention.
Furthermore, by so using DNAs of this invention, genetic markers associated with the KGF gene, such as restriction fragment length polymorphisms (RFLPs), may be identified and associated with inherited clinical conditions involving this or other nearby genes. Such variants include, as is appreciated, any DNA sequence which hybridizes to the DNA sequence encoding KGF
protein or polypeptide fragments thereof or any DNA
sequence which has sufficient closeness in homology to have the same functional encoding capacity of DNA
encoding KGF.
This invention also includes modified forms of KGF DNAs. According to a preferred embodiment of this aspect of the invention, such modified DNAs may encode KGF-like proteins comprising segments of amino acid sequences of KGF and at least one other member of the FGF peptide family. Thus, for example, since there is no significant N-terminal homology between the secreted form of KGF and analogous positions in other FGF-related proteins, polypeptides with novel structural and functional properties may be created by grafting DNA segments encoding the distinct N-terminal segments of another polypeptide in the FGF family onto a KGF DNA segment in place of its usual NHZ-terminal sequence.
The polypeptide chimeras produced by such modified DNAs are useful for determining whether the ~~ ~ ~~~8 KGF NH2-terminal domain is sufficient to account for its unique target cell specificity. Studies on chimeras should also provide insights into which domains contribute the different effects of heparin on their biologic activities. As is understood, one or more regions of the KGF peptide act as function domains) for the protein to render it biologically useful. Such function domains are the critical sections of the protein in providing a growth function, receptor binding and/or medical treatment properties.
Usually such functional domains comprise at least 10 amino acid residues and may be the same as or functionally the same as the amino acid sequence of the normal KGF polypeptide fragment. By functionally the same, it is understood to include embodiments which provide the same result by non-critical substitution of amino acid residues in the polypeptide or the cDNA
sequence encoding the polypeptide.
Indeed, the utility of this approach has already been confirmed by the successful engineering and expression of a chimeric molecule in which about 40 amino acids from the NHZ-terminus of the secreted form of KGF (beginning with the amino terminal cys residue of the mature KGF form, numbered 32 in Fig. II-1, and ending at KGF residue 78, arg) is linked to about 140 amino acids of the C03-terminal core of aFGF (beginning at residue 39, arg, and continuing to the C-terminal end of the aFGF coding sequence. The sequence for aFGF
is set out in Fig. II-1D. This chimeric product has a target cell preference for keratinocytes, like KGF, but lacks susceptibility to heparin, a characteristic which parallels that of aFGF rather than KGF. This novel KGF-like growth factor may have advantages in clinical applications where administration of an epithelial-specific growth factor is desirable in the presence of heparin, a commonly used anticoagulant. Further details of the construction of this chimeric molecule and the properties of the polypeptide are described in Experimental Section II.
Other DNAs of this invention include the following recombinant DNA molecules comprising a KGF
cDNA and any of the following exemplary vector DNAs: a bacteriophage a cloning vector (apCEV9); a DNA
sequencing plasmid vector (a pUC variant); a bacterial expression vector (pKK233- 2); suitable yeast and plant cell expression vectors; or a mammalian expression vector (pI~IT/neo). Such recombinant DNAs are exemplified by 25a constructs described in detail in the Experimental Sections.
Most preferred recombinant molecules include the following: molecules comprising the coding sequence for the secreted form.of KGF and a bacterial expression vector (e.g., pKK233-2) or a cDNA encoding the entire primary translation product (including the Niit-terminal signal peptide) and a mammalian expression vector . (exemplified by pl~iT) capable of expressing inserted DNAs in mammalian (e. g., NIIi/3T3) cells.
Construction of recombinant DNAs containing KGF DNA and a bacterial expression vector is described in Experimental Section II.
In brief, KGF cDNA was expressed to produce polypeptide in E.coli by placing its coding sequence under control of the hybrid t~ promoter in the plasmid expression vector pKK233-2 (II-31) .
Construction of recombinant DNAs comprising KGF DNA and a mammalian vector capable of expressing inserted DNAs in cultured human or animal cells, can be carried out by standard gene expression technology using methods well known in the art for expression of such a relatively simple polypeptide. One specific embodiment of a recombinant DNA of this aspect of the present ....
invention, involving the mammalian vector pMMT, is described further below in this section under recombinant cells of this invention.
DNAs and sense strand RNAs of this invention can be employed, in conjunction with protein production methods of this invention, to make large quantities of substantially pure KGF or KGF-like proteins.
Substantially pure KGF protein thus produced can be employed, using well-known techniques, in diagnostic assays to determine the presence of receptors for this protein in various body fluids and tissue samples.
Accordingly, this invention also comprises a cell, preferably a bacterial, yeast, plant, insect or mammalian cell, transformed with a DNA of the invention, wherein the transforming DNA is capable of being expressed. In a preferred embodiment of this aspect of the invention, the cell transformed by the DNA of the invention produces KGF protein in a fully mitogenic form. Most preferably, these proteins will be of a secreted form (i.e., lacking an apparent signal sequence). These protein factors can be used for functional studies, and can be purified for additional biochemical and functional analyses, such as qualitative and quantitative receptor binding assays.

Recombinant E. coli calis have been constructed in a bacterial expression vector, pRK233-2, for production of RGF, as detailed in Experimental Section II. In summary, several recombinant bacterial clones were tested for protein production by the usual small scale methods. All recombinants tested synthesized a protein that was recognized by antibodies raised against an amino-terminal RGF peptides (sss 10. below). One recombinant was grown up in a one liter culture which produced recombinant KGF that efficiently stimulated thymidine incorporation into DNA of BALB/MK ksratinocyts cells, but was only marginally active on NIFI/3T3 fibroblasts.
Half-maximal stimulation of the HALB/MR cells in the standard keratinocyte bioassay was achieved with a concentration of between 2 to 5 ng/ml, compared to a concentration of 10 to 15 ng/ml for RGF purified from M426 cells.
One liter of bacterial cells yielded approximately 50 ~g of Mono-S purified recombinant RGF. It will be apparent to those skilled in the art of gene expression that this initial yield can be improved substantially without undue experimentation by application of a variety known recombinant DNA technologies.
Recombinant mammalian (NIN/3T3 mouse) cells have also been constructed using the entire KGF cDNA coding sequence (including the NHZ-terminal signal peptide) and the vector pMMT/neo, which carries mouse metallothionina (MrIT) promoter and the selective marker gene for neomycin resistance. The cells are being evaluated for KGF production, particularly for secretion of the mature form (lacking signal peptide) produced by human fibroblasts, using bioassays of the present invention. This same vector and host cell combination has been used successfully to express several other similar recombinant polypeptides, including high levels of Platelet-Derived Growth Factor (PDGF) A and H
chains (II-32). Accordingly, it will be recognized by those skilled in the art that high yields of recombinant RGF can be achieved in this manner, using the aforementioned recombinant DNAs and transformed cells of this invention.
Ultimately, large-scale production can be used to enable clinical testing in conditions requiring specific stimulation of epithelial cell growth. Materials and methods for preparing pharmaceutical compositions for administration of polypeptidas topically (to skin or to the cornea of the eye, for example) or systemically are well known in the art and can be adapted readily for ~fl3r~~~~
administration of KGF and KGF-like peptides without undue experimentation.
This invention also comprises novel antibodies made against a peptide encoded by a DNA
segment of the invention. This embodiment of the invention is exemplified by several kinds of antibodies which recognize KGF. These have been prepared using standard methodologies well known in the art of experimental immunology, as outlined in Experimental Section II. These antibodies include: monoclonal antibodies raised in mice against intact, purified protein from human fibroblasts; polyclonal antibodies raised in rabbits against synthetic peptides with sequences based on amino acid sequences predicted from the KGF cDNA sequence [preferably sequences comprising 10 to 20 amino acids, such as exemplified by a peptide with the sequence of KGF residues 32-45, namely, NDMTPEQMATNVR (using standard one-letter code for amino acid sequences; see Fig. II-1)]; polyclonal antibodies raised in rabbits against both naturally secreted KGF from human fibroblasts and recombinant KGF
produced in E. coli (see above).
All tested antibodies recognize the recombinant as well as the naturally occurring KGF, either in a solid-phase (ELISA) assay and/or in a Western blot. Some exemplary antibodies, which are ~~~g~jg preferred antibodies of this invention, appear to neutralize or inhibit mitogenic activity of KGF in the BALB/MK bioassay.
Fragments of antibodies of this invention, such as Fab or F(ab)~ fragments, which retain antigen binding activity and can be prepared by methods well known in the art, also fall within the scope of the present invention. Further, this invention comprises pharmaceutical compositions of the antibodies of this invention, or active fragments thereof, which can be prepared using materials and methods for preparing pharmaceutical compositions for administration of polypeptides that are well known in the art and can be adapted readily for administration of KGF and KGF-like peptides without undue experimentation.
These antibodies, and active fragments thereof, can be used, for example, for detection of KGF
in bioassays or for purification of the protein factors. They may also be used in approaches well known in the art, for isolation of the receptor for KGF, which, as described in Experimental Section II, appears to be distinct from those of all other known growth factors.
Those preferred antibodies, and fragments and pharmaceutical compositions thereof, which neutralize or inhibit mitogenic activity of KGF for epithelial 2~~~~~$
cells, as indicated by the BALB/MK assay, for instance, may be used in the treatment of clinical conditions characterized by excessive epithelial cell growth, including dysplasia and neoplasia (e.g., psoriasis, or malignant or benign epithelial tumors or benign mixed stromal/epithelial tumors).
This invention further comprises novel bioassay methods for detecting the expression of genes related to DNAs of the invention. In some exemplary embodiments, DNAs of this invention were used as probes to determine steady state levels of related mRNAs.
Methods for these bioassays of the invention, using KGF
DNAs, and standard Northern blotting techniques, are described in detail in Experimental Section II.
One skilled in the art will recognize that, without undue experimentation, such methods may be readily applied to analysis of gene expression for KGF-like proteins, either in isolated cells or various tissues. Such bioassays may be useful, for example, for identification of various classes of tumor cells or genetic defects in the epithelial growth processes.
In accordance with the invention, KGF is also as potent as EGF in stimulating proliferation of primary or secondary human keratinocytes in tissue culture. Exposure of KGF or EGF stimulated keratinocytes to 1.0 mM calcium, an inducer of differentiation, leads to cessation of cell growth.
However, immunological analysis of early and late markers of terminal differentiation, in the form of the proteins Kl and filaggrin, reveal striking differences in the keratinocytes growth in the presence of these two growth factors. With KGF the normal differentiation response is evident as demonstrated by association with the expression of both markers whereas their appearance was retarded or blocked by EGF.
Furthermore, TGFa which also interacts with the EGF
receptor gave similar response to that observed with EGF. Such significant differences in treated human keratinocytes distinguishes KGF from the EGF family of growth factors. This information confirms efficacy of KGF in stimulating the proliferation of human epithelial cells and simultaneously permit the normal differentiation of the cells.
KGF has specific high affinity binding to surface receptors on intact BALB/MK mouse epidermal keratinocytes, but not on NIH/3T3 fibroblasts. KGF
binding on BALB/MK cells competed efficiently with aFGF
and with 20-fold lower efficiency with bFGF. In contrast, aFGF and bFGF bind competitively on both BALB/MK keratinocytes and NIH/3T3 fibroblasts.
Covalent affinity cross-linking of lzsl-KGF to its receptor on BALB/MK cells reveals two species of 115 2~3~~~~
.~~..
and 140 kDa. KGF stimulates the rapid tyrosine phosphorlation of a 90 kDa protein in BALB/N~ cells but not in the NIH/3T3 fibroblasts. Hence, the BALB/MK
keratinocytes possess high affinity KGF receptors to which FGF may also bind, however, these receptors are distinct from the receptors for aFGF and bFGF on NIH/3T3 fibroblasts which fail to interact with KGF.
A cDNA encoding a KGF receptor from the BALB/MK cells was isolated and sequenced. The amino acid sequence deduced from the coding region of the KGF
receptor is set out in Fig. V-2A. The cDNA of the receptor has a variety of additional uses. For example, the receptor cDNA and KGF binding analysis, as described above, could have a variety of uses, for example, in diagnostic studies wherein knowledge of KGF
receptor levels could be of prognostic or therapeutic benefit. Furthermore, a functional fragment of the receptor protein can be useful for treating cell proliferative disorders where excessive activation of receptor molecules is associated with the ailment to be treated. The receptor protein fragment would be biologically functional to bind and thereby inactivate excess KGF in the mammal circulatory system.
Without further elaboration, it is believed that one of ordinary skill in the art, using the preceding description, and following the methods of the 33a Experimental Sections below, can utilize the present invention to its fullest extent. The material disclosed in the Experimental Sections, unless otherwise indicated, is disclosed for illustrative purposes and therefore should not be construed as being limitive in any way of the appended claims.
33 b . EJCPERIMENTaL BBS~rTny I
.1.....
IDENTIFIC1,TION 7~ND CHARACTERI871TION OF 71 NOQEL
GROWTH FliICTOR SPECIFIC FOR EPITHBLIlIL CELLS
This section describes experimental work leading to identification of a growth factor specific for epithelial cells in conditioned medium of a human embryonic lung fibroblast cell line. The factor, provisionally termed keratinocyte growth factor (KGF) because of its predominant activity on this cell type, was purified to homogeneity by a combination of ultrafiltration, heparin-Sepharose affinity chromatography and hydrophobic chromatography on a C~ reversed-phase HPLC column, according to methods of this invention. RGF was found to be both acid and heat labile, and consisted of a single polypeptide chain with an apparent molecular weight of approximately 28,000 daltons.
Purified RGF was a potent mitogen for epithelial cells, capable of stimulating DNa synthesis in quiescent H7~rL8/MR epidermal keratinocytes by more than 500-fold with activity detectable at 0.1 nM
and maximal at 1.o nM. Lack of mitogenic activity on either fibroblasta or endothelial cells indicated that KGF possessed a target cell specificity distinct from any previously characterized growth factor. Microsequencing _. revealed an amino-terminal sequence containing no significant homology to any known protein. The .....
release of this novel growth factor by human embryonic fibroblasts indicates that KGF plays a role in mesenchymal stimulation of normal epithelial cell proliferation.
Precaration of Conditioned Media. An early passage of M426 human embryonic fibroblasts (I-8) was plated onto 175 cm2 T-flasks and grown to confluence over 10-14 days in Dulbecco's modified Eagle's medium (DMEM; GIBCO) supplemented with 10~ calf serum (GIBCO). Once confluent, the monolayers were cycled weekly from serum-containing to serum-free medium, the latter consisting of DMEM alone. The cells were washed twice with 5 ml of phosphate buffered saline prior to addition of 20 ml of DMEM. After 72 hrs, culture fluids were collected and replaced with 35 ml of serum-containing medium. The conditioned medium was stored at -70'C until further use.
Ultrafiltration.~ Approximately ten liters of conditioned medium were thawed, prefiltered through a 0.50 micron filter (Millipore HAWP 142 50~ and concentrated to 200 * trade mark ml using the Pellicon cassette system (Millipore XX42 OOK 60) and a cassette having a 10 kDa molecular weight cutoff (Millipore PTGC 000 05~. After concentration, the sample was subjected to two successive rounds of dilution with one liter of 20 mM Tris-HC1, pH 7.5/0.3 M
NaCl, each followed by another step of ultrafiltration with the Pellicon system. Activity recovered in the retentate was either immediately applied to heparin-Sepharose resin or stored at -70°C.
Heparin-Sepharose Affinity Chromatography (HSAC1 The retentate from ultrafiltration was loaded onto heparin-Sepharose resin (Pharmacia) which had been equilibrated in 20 mM Tris-HC1, pH 7.5/0.3 M NaCl. The resin was washed extensively until the optical density had returned to baseline and then subjected to a linear-step gradient of increasing NaCl concentration. More particularly, approximately 150 ml of ultrafiltration retentate derived from 5 liters of M426 conditioned medium were loaded onto a heparin-Sepharose column (6 ml bed volume) in 1 hr. After washing the column with 150 ml of the equilibration buffer, 20 mM Tris-HC1, pH 7.5 0.3 M NaCl, the retained protein (<5% of the total protein in the retentate) was eluted with a modified linear gradient of increasing NaCl concentration. Fraction * trade mark ,., ~~~~~9 size was 3.8 ml and flow rate during gradient elution was 108 ml/hr. Two ~1 of the indicated fractions were transferred to microtiter wells containing a final volume of 0.2 ml for assay of 3H-thymidine incorporation of BALB/MK cells as described in the Methods. After removing aliquots from the fractions for the thymidine incorporation bioassay, selected fractions were concentrated ten- to twenty-fold with a Centricon-l0 microconcentrator (Amicon) and stored at -70°C.
Reverse-Phase HPLC (RP-HPLC~~ Active fractions (0.6 M
NaCl pool) from the HSAC were thawed, pooled and further concentrated with the Centricon-10 to a final volume of __<200 ~L. The sample was loaded onto a Vydac HPLC column (The Separations Group, Hesperia, CA) which had been equilibrated in 0.1% trifluoroacetic acid (TFA, Fluka)/20% acetonitrile (Baker, HPLC grade) and eluted with a linear gradient of increasing acetonitrile. Aliquots for the bioassay were immediately diluted in a 10-fold excess of 50 ~g/ml BSA
(Fraction V, Sigma)/20 mM Tris-HC1, pH 7.5. The remainder of the sample was dried in a Speed-Vac (Savant) in preparation for structural analysis.
A preferred technique for the above was to elute active fractions from heparin-Sepharose with 0.6M
NaCl process with the Centricon-10 and load directly CJ s,~ ~,, "....
onto a C4 Vydac column (4.6 x 250 mm) which had been equilibrated in 0.1% trifluoroacetic acid/20%
acetonitrile (ACN). After washing the column with 4 ml of equilibration buffer, the sample was eluted with a modified linear gradient of increasing percentage of ACN. Fraction size was 0.2 ml and flow rate was 0.5 ml/min. Aliquots for the assay of 3H-thymidine incorporation in BALB/MK cells were promptly diluted 10-fold with 50 ~g/ml bovine serum albumin/20 mM Tris-HC1, pH 7.5, and tested at a final dilution of 200-fold. (B) NaDodSo4/PAGE analysis of selected fractions from the C4 chromatography shown in panel A. Half of each fraction was dried, redissolved in NaDodSo4/2-mercaptoethanol, heat denatured and electrophoresed in a 14% polyacrylamide gel which was subsequently stained with silver. The position of each molecular weight marker (mass in kDa) is indicated by an arrow. (C) DNA
synthesis in BALB/MK cells triggered by the fractions analyzed in Panel B. Activity is expressed as the fold stimulation over background which was 100 cpm.
Molecular Sieve HPLC
Approximately 50 ~l of the twice concentrated heparin-Sepharose fractions were loaded onto a TSK-G3000SW Glas-Pac Column (LKB, 8 x 300 mm) which had been equilibrated in 20 mM Tris-HC1, pH 6.8/0.5M NaCl.
The sample was eluted in this buffer at a flow rate of 3 7a 0.4 ml/min and 0.2 ml fractions were collected.
Aliquots of 2 ~1 were transferred to microtiter wells containing a final volume of 0.2 ml for assay 3H-thyminidine incorporation in BALB/MK cells. The elution positions of molecular weight markers (mass in kDa) were as indicated by the arrows. After removing aliquots for the bioassay, the fractions were stored at -70°C.
A preferred technique for the above is to load approximately 50 ~1 of a Centricon-processed, 0.6M
NaCl pool from HSAC onto a LKB Glas-Pac TSK G3000SW
column ( 8 x 300 mm), previously equilibrated in 20 mM
Tris-HC1, pH 6.8/0.5M NaCl, and eluted as 0.2 ml fractions at a flow rate of 0.4 ml/min.
NaDodSO;~-Polyacrylamide Gel Electrophoresis (NaDodSO,,/Pacte) Polyacrylamide gels were prepared with NaDodS04 according to the procedure of Laemmli (I-9). Samples were boiled for 3 min in the presence of 2.5% 2-mercaptoethanol (vol/vol). The gels were fixed and stained with silver (I-10) using the reagents and protocol from BioRad. Molecular weight markers were from Pharmacia.
37 b DNA Synthesis Stimulation, Ninety-six .---. well microtiter plates (Falcon No. 3596) were precoated with human fibronectin (Collaborative Research) at 1 ~g/cmt prior to seeding with HALH/MK cells. Once confluent, the cells were maintained for 24-72 hr in serum-free medium containing 5 ~g/ml transferrin (Collaborative . , Research) and 30 nM NaZSe03 (Baker) .
Incorporation of ~Ii-thymidine (5 uCi/ml final concentration, NEN) into DNA was measured during a 6 hr period beginning at 16 hrs following addition of samples. The assay was terminated by washing the cells once with ice cold phosphate-buffered saline and twice with 58 trichloroacetic acid. The precipitate was redissolved in 0.25 M
NaOH, transferred into liquid scintillation fluid (Hiofluor, NEN) and counted.
Stimulation of DNA synthesis was monitored as described above for BALB/MK calls on a variety of other call lines. NIH/3T3 fibroblasts (I-11) were available fros the National Institutes of Health, while CCL208 Rhesus monkey bronchial epithelial cells (I-12) were obtained frog the American Type Culture Collection. The H5/589 human mammary epithelial cell line, prepared as described in (I-13), was obtained from Martha Stampfer (University of California, Berkeley). The mammary cells were 3s 2038398 grown in RPMI 1640 supplemented with 10~ fet~1 ,,.,,. calf serum and 4 ng/ml EGF. When maintained in serum-free conditions, the basal medium was DMEM.
Primary cultures of human saphenous vein endothelial cells were prepared and maintained as described elsewhere (I-14). Epidermal growth factor and insulin were from Collaborative Research. Acidic FGF and bFGF were obtained from California Biotechnology, Inc. Recombinant TGFa was obtained from Genentech, Inc. Media and serum were either from GIBCO, Biofluids, Inc. or the NIIi media unit.
Proliferation Assav. Thirty-five mm culture dishes were precoated sequentially with poly-D-lysine (20 ~g/cmt) (Sigma) and human fibronectin, and then seeded with approximately 2.5 x 10~ HALS/MK cells. The basic medium was a 1:1 mixture of Eagle's low Cai' minimal essential medium and Ham's F-12 medium, supplemented with 5 ~cg/ml transferrin, 30 nM NaiSe03 and 0.2 mM
ethanolamine (Sigma). Medium was changed every 2 or 3 days. After 10 days, the cells ware fixed in formalin (Fisher Scientific Co.) and stained with Giemsa (Fisher Scientific Co.).
Protein microseauencinc. Approximately 4 ~g (_150 pmol) of protein from the active fractions of the C~ column were redissolved in 50~
TFA and loaded onto an Applied Biosystems gas-39 2 p 3 8 3 9 8 phase protein sequenator. Twenty rounds of Edman degradation were carried out and identifications of amino acid derivatives were made with an automated on-line HPLC (Modal 120A, Applied Hiosystems).
Growth Factor DetaCtint~ anrl Igplatinw, - ~ Preliminary screening of conditioned media from various cell lines indicated that media from some fibroblast lines contained mitogenic activities detectable on both BALB/MK and NIH/3T3 cells.
Whereas boiling destroyed the activity on BALH/MR, mitogenic activity on NIH/3T3 remained intact. Based on the known heat stability of EGF
(I-15) and TGFa (I-16), it was reasoned that the BALH/IrQt mitogenic activity might be due to an agent different from these known epithelial growth factors.
M426, a human embryonic lung fibroblast lines, was selected as the most productive source of this activity for purification of the putative growth factor(s). Ultrafiltration with the Pellicon systea provided a convenient way of reducing the sample volume to a suitable level for subsequent chromatography. Various combinations of sieving, ion exchange and isoelectric focusing chromatography were tried during the development of a purification scheme, but all resulted in unacceptably low yields.
On the other hand, heparin-Sephaross affinity chromatography (HSAC), which has been employed in the purification of other growth factors (I-17--, I-22), proved to bs useful as an early purification step in the present invention.
While estimates of recovered specific activity were uncertain at this stags because of the likely presence of other factors, the apparent yield~of activity was 50-708 with a corresponding enrichment of approximately 1000 fold.
As shown in Fig. I-1, greater than 908 of the HALB/MK mitogenic activity eluted from the HSAC column with 0.6M NaCl. This peak of activity was not associated with any activity on NIH/3T3 cells (data not shown). A much smaller peak of HALB/MR mitogsnic activity consistently emerged with 0.8 - 1.2M NaCl. ' Due to the reproducibility of the HSAC
pattern, active fractions could b~ identified pr~sumptiv~ly on the basis of the gradient and optical density profile. Prompt concentration of i0-ZO fold with the Centricon-10 was found to be essential for stability, which could be maintained subsequently at -70'C for several months.

Final purification was achieved by RP-HPLC
with a C~ Vydac column, a preparative method suitable for amino acid sequence analysis. While the yield of activity from the C~ step was usually only a few percent, this loss could ba attributed to the solvents employed. In other experiments, exposure to 0.1~ TFA/50~ acetonitrile for 1 hr at room temperature reduced the mitogenic activity of the preparation by 98~. Nonetheless, as shown in Fig. I-2, a single peak of HALB/MK stimulatory activity was obtained, coinciding with a distinct peak in the optical density profile. The peak fractions produced a single band upon NaDodSO~/PAGE and silver staining of the gel (Fig.
I-28), and the relative mitogenic activity of each tested fraction (Fig. I-2C) correlated well with the intensity of the bands across the activity profile.
An alternative purification step to the 2o HPLC technique described above, using sieving chromatography with a TSK G3000SW GlasPac column run in aqueous solution near physiologic pH, resulted in a major peak of activity in the H~IhB/MIC bioassay (Fig. I-3) . This preparation was almost as pure as the one obtained from RP-HPLC as judged by silver-stained NaDodSOi/PAGE
(data not shown) but provided a far better recovery of activity (Table I-i). The TSR-purified material was used routinely for biological studies as it had a higher specific activity.
In both types of purified preparations (i.e., purified by HPLC or molecular sieving), the profile of mitogenic activity was associated with a distinct band on NaDodSO~/PAGE which appeared to be indistinguishable in the two preparations.
phvsica~ and H=~,,.,.s~'~ ~r,~rast.~ri~ation of the Growth Factor. The purified factor had an estimated molecular weight of about 28 kDa based on NaDodSO~/PAGE under reducing (Fig. I-2) and non-reducing conditions (data not shown).. This value was in good agreement with its elution position on two different sizing columns run in solvents expected to maintain native conformation (TSK-63000-SW, Fig. I-3, and superose-12, data not shown). From these data, the mitogen appears to consist o! a single polypaptids chain with a molecular weight of 25-30 kDa.
The heat and acid lability o! the mitogsnic activity wars demonstrated using the HALH/MR mitogenesis bioassay. while activity was unaffected by a l0 min incubation at 50'C, it was reduced by 688 after 10 min at 60'C and was undetectable after 3 min at 100'C. Exposure to 0.5M acetic acid for 60 min at room temperature 2a3~~~$
resulted in a decline in activity of 15% of the control. In comparison, the mitogenic activity of the known growth factor, EGF, was not diminished by any of these treatments.
The dose response curve for the purified growth factor depicted in Fig I-4 illustrates that as little as 0.1 mM led to a detectable stimulation of DNA
synthesis. Thus, the activity range was comparable to that of the other growth factors analyzed to date. A
linear relationship was observed in the concentration range 0.1 - 1.0 nM with maximal stimulation of 600-fold observed at 1.0 nM. The novel factor consistently induced a higher level of maximal thymidine incorporation than EGF, aFGF or bFGF in the BALB/MK
keratinocytes (Fig. I-4). Incorporation of 3H-thymidine into trichloracetic acid-insoluble DNA, expressed as fold stimulation over background, was measured as a function of the concentration of the indicated growth factors. Background values with no sample added were 150 cpm. The results represent means values of two independent experiments. Replicates in each experiment were within 10% of mean values. TSK-purified mitogen, ~ ~ EGF, ~ ~, aFGF, bFGF, 0 0 The distinctive target cell specificity of this factor was demonstrated by comparing its 2~~~~~8 activities on a variety of cell types with those of other growth factors known to possess epithelial cell mitogenic activity. As shown in Table I-2, the new isolated factor exhibited a strong mitogenic effect on BALB/MK but also induced demonstrable incorporation of thymidine into DNA of the other epithelial cells tested. In striking contrast, the factor had no detectable mitogenic effects on mouse (or human data not shown) fibroblasts or human saphenous vein endothelial cells.
By comparison, none of the other known growth factors appeared to preferentially stimulate keratinocytes. TGFa and EGF showed potent activity on fibroblasts, while the FGFs were mitogenic for endothelial cells as well as fibroblasts (Table I-2).
Because of its specificity of epithelial cells and the sensitivity of keratinocytes in particular, the novel mitogen was provisionally designated as keratinocyte growth factor (KGF).
To establish that KGF not only would stimulate DNA synthesis but would also support sustained cell growth, the ability of BALB/MK cells to grow in a fully-defined, serum-free medium supplemented with this growth factor was assessed. With reference to Figure I-5, cultures were plated at a density of 2.5 x104 cells per dish on 35 mm Petri dishes precoated ,~. ~~~~~y~~8 with poly-D-lysine/fibronectin in a 1:1 mixture of Eagle's minimal essential medium and Ham's F12 medium supplemented with transferrin, Na2Se03, ethanolamine and the growth factors indicated below. After 10 days, the plates were fixed and stained with Giemsa. Key: a) no growth factor; b) EGF alone; c) insulin alone; d) KGF
alone; e) EGF + insulin. Final concentrations of the growth factors were as follows: EGF, 20ng/ml; insulin, ~Cg/ml; and KGF, 40 ng/ml. As shown in Fig. I-5, KGF
10 served as an excellent substitute for EGF but not insulin (or insulin-like growth factor I) in this chemically defined medium. Thus, KGF appears to act through the major signalling pathway shared by EGF, aFGF and bFGF for proliferation of BALB/MK cells.
Further aspects of the impact of KGF on cell proliferation and in particular on human keratinocyte growth is discussed in Experimental Section III.
Microsequencinq Reveals a Unique N-Terminal Amino Acid Sequence of KGF. To further characterize the growth factor, approximately 150 pmol of C4-purified material were subjected to amino acid sequence analysis. A single sequence 4 5a ~~~i)e3~~
was detected with unambiguous assignments made ,,~, for cycles 2-13, as follows: X-Asn-Asp-Met-Thr-Pro-Glu-Gln-Met-Ala-Thr-Asn-Val. High background noise precluded an assignment for the first position which is, therefore, indicated by an X.
A computer search using the FASTP
program (I-24) revealed that the N-terminal amino acid sequence of KGF showed no significant homology to any protein in the National Biomedical Research Foundation data bank, thus supporting the novelty of this epithelial growth factor.
The studies described in this Experimental Section identified a human growth factor which has a unique specificity for epithelial cells. By employing ultrafiltration, HSAC and RP-HPLC or TSR sieving Chromatography according to the present invention, a quantity sufficient to p~rmit d~tailsd characterization of the physical and biological proptrti~s of this molwcul~ was isolated.
A single silver-stained band corresponding to a molecular w~ight of about 28,000 daltons was detected in the active fractions from RP-HPLC, and the intensity of the band was proportional to the level of mitogenic activity in these fractions. A band indistinguishable from that obtained by RP-HPLC
was seen in the active fractions from TSR
chromatography. The purified protein stimulated DNA synthesis in epithelial calls at sub-nanomolar concentrations, but failed to induce any thymidine incorporation in ffbroblasts or .. ~~ endothelial calls at comparable or higher to concentrations (up to 5 nM). This distinctive target cell specificity combined with the single novel N-terminal amino acid sequence determined from the purified molecule lead to the conclusion that RGF represents a new growth !actor.
In a chemically defined ~ediun the purified factor was able to complement the insulin-like growth factor I/insulin growth requirement of BALB/MR cells and therefore must act through a signal tranaduction pathway shared with EGF, TGFa and the FGFs. Moreover, the new factor was more potent than any of the known epithelial cell mitogens in stimulating thymidine incorporation in BALB/MR cell. Preliminary evidence indicates that this factor is also capable of supporting proliferation of secondary cultures of human keratinocytes (data not shown).
Handling and storage o! IcGF were problematical during its purification. Besides its inherent !ability to acid and heat, it was unstable to lyophilization or dialysis. After .
IiSAC, complete loss ~f activity occurred within 24 hr despite the use of carrier proteins, heparin, protease inhibitors, siliconized tubes or storage at either 4' or -20'C. Only concentrating the sample at this stage could preserve its activity.
Furthermore, in order to transfer the dried, purified factor it was necessary to utilize either strong acid or detergent, consistent with an adsorptive tendency or insolubility. Thus, for preservation of activity, the purified factor was maintained in solution at high concentrations at -70'C where it remained stable for several months.
The ability of RGF to bind heparin may signify a fundamental property of this factor that has a bearing on its function in ri~o. Growth factors with heparin-binding properties include aFGF (I-20--I-22) bFGF (I-19, I-22) granulocyts/macrophags colony stipulating factor and interlsukin 3. (I-25) Each of these is produced by stromal cells (I-25--I-27). Sucn !actors appear to be deposited in the extracellular matrix, or on protsoglycans coating the stromal cell surface (I-25, I-28). It has been postulated that their storage, release and contact with specific target cells are regulated 2~3~~~~~
by this interaction (I-25, I-28). While mesenchymal-derived effectors of epithelial cell proliferation have also been described (I-29--I-31), their identities have not been elucidated.
Its heparin-binding properties, release by human embryonic fibroblast stromal cells, and epithelial cell tropism provide KGF with all of the properties expected of such a paracrina mediator of normal epithelial cell growth.
The partial amino acid sequence determined for this new growth factor has enabled molecular cloning of its coding sequence and determination of its structural relationship to known families of growth factors, as described in Experimental Section II, below.
I-1. James, R. and Bradshaw,~R.A. (1984) Ann.
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2Q~~~~~

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~~C~ERIMEIdT7ilL BECTION II
cDNA SEQOENCE OF
A NOVEL EPITHELIAL CELL SPECIFIC GROWTH FACTOR
DEFINEB A NEW MEMBER OF THB FGF FAMILY
Work in the previous Experimental Section I identified and purified a novel heparin-binding growth !actor, designated keratinocyte growth factor (RGF), which is particularly active on keratinocytes and appears to be specific for epithelial cells. This second Experimental Section describes the isolation and characterization of cDNA clones encoding RGF, using synthetic oligonucleotides, based upon the experimentally determined NHi-terminal amino acid sequence, as hybridization probes. Nucleotide sequence analysis identified a 582-by open reading frame which would code for a 194-amino acid polypsptids that is between 41~ and 33~
identical to the heparin-binding acidic and basic fibroblast growth factors (FGFs), and the related products of the hs: and ini-1 oncogenes. Tns x~r-gene RNA transcript is expressed in normal libroblasts of both embryonic and adult origin, but not in epithelial, endothelial or glial calls. Thus, RGF appears to bs normally expressed by the mesanchyms, indicating a role in the regulation of epithelial cell proliferation.

~~~~~_5~~$
"° Isolation of cDNA clones. The purification and N-tenainal sequencing of KGF has been previously described (see Experimental Section I, above and II-3j. Pools (50 pmole) of deoxyoligonucleotides described under Results were 5' end-labelled using 83 pmole of t ~P-ATP
(3000 Ci/mmole, Amersham) and 10 units of T4 polynucleotide kinase. The recombinant phage carrying cDNA clones were replica plated onto nitrocellulose filters and hybridized with 32P-labelled deoxyoligonucleotides in 20~
formamide, 10~ dextran sulphate, 10 mM Tris-HC1 (pH 7.5), 8 x SSC, 5x Denhardt's and 50 ~g/ml denatured salmon sperm DNA, overnight at 42'C.
Filters were washed in 0.5 x SSC, 0.1~ SDS at 50~C and exposed to Rodak X-omat AR tile.
DNA sequencinc. The nucleotide sequence of the KGF cDNA was determined bx thw dideoxy . 20 chain termination method (II-26), of overlapping restriction fragments, subclonwd into ptJC vectors (ii-2~) vector for RGF cDNA. RGF cDNA tncoding the mature, secreted form of the polypeptida was placed under control of the hybrid ~ promoter in the plasmid expression vector pRR233-2 (II-2~,~~~~~~
31), as follows. To accomplish this, a specific length of KGF cDNA that contained the information to code for the mature KGF molecule (i.e., without its signal peptide) was amplified using the polymerise chain reaction (PCR) technique (II-32). The fragment was directionally inserted between two sites in the vector, namely the Ncol site, made blunt ended by S1 nuclease digestion, and the HindIII site, using standard recombinant DNA methodology. The entiS of Zua awr v......-.
produced by the PCR method were as follows: the 5' end was blunt and began with an ATG codon, followed by the codon TGC for cys residue, number 33, which is the amino terminal residue of the mature form of RGF (see Fig. II~1), and then the entire RGF coding sequence. The stop colon, TAA, and the four bases immediately following, TTGC, wars also included on the 3' end of the cDNA.
The primer used in the 8CR method to direct DNA
synthesis to the desired position on the 3' end o! the cDNA included a XindIII site for insertion o= the amplilied cDNA into the vector DNA.
~~-..,~t ~ en o! swt i ~,~ i es aaain~t RGF and °~a="°~lAted ~~ Monoclonal antibodies were raised in mice against intact, purified protein from human fibroblasts using 5 or mots subcutaneous injections. Test bleeds were screened with a solid-phase (ELISA) assay using highly purified KGF from human epithelial cells as antigen. Hybridomas were prepared by routine methods and supernatants were screened with the ELISA assay to detect KGF-reactive antibodies.
Positive clones were serially subcloned by the usual methods, and selected subclones were grown as ascites tumors in mice for production of large amounts of antibodies. Antibodies were purified from ascites fluids employing standard techniques (e. g., hydroxyapatite or immunoaffinity resins).
Polyclonal antibodies against a synthetic peptide were raised in rabbits by standard methods, as follows. The peptides were made by solid phase technology and coupl~d to thyroglobulin by reaction with glutaraldehyde.
Serial subcutaneous injections were made and test bleed wer~ acre~ned by ELISA as will as other techniques, including Wasters blot analysis and mitogsnasis bioassay. IgG immunoglobulins ware isolated by affinity chromatography using immobilized protein G.
Polyclonal antibodies were raised in rabbits against both naturally s~crtted KGF from human fibroblasts and r~combinant KGF produced in E.coli (see next section), using the following protocol: _ i) Initial injection and first boost were '' administered in the inguinal lymph nodes:
ii) subsequent boosts were made intramuscularly.
Screening o! test bleeds included ELISA as well as Western blot analysis and mitogenesis bioassay, and IgG was purilied as !or antibodies against synthetic peptides, above.

isolation of cDNA clones encodinq_the novel growth factor. To search for cDNA clones corresponding to the KGF coding sequencs, two pools of oligonucleotides with lengths of 26 bases were generated based upon a nine-amino acid sequence, Asn-Asp-Met-Thr-Pro-Glu-Gln-Mst-Ala, as .. determined by microsequencing of purified KGF
(see Experimental Section I, above and reference II-3). One oligonucleotide pool contained a mixture of all 256 possible coding sequences for the nine amino acids, while the other contained inosine residues at the degenerate third position of the codons for Thr and Pro.
This latter design reduced the number of possible coding sequences in the pool to 16.
Inosine in a tRNA anticodon can form hydrogen bonds with A, C or U (II-4), and oligonucleotides that contain dsoxyinosins have bean shown to hybridize efficiently with the corresponding cDNA
(II-5) .
A cDNA library was constructed in a cDNA
cloning vector, pCEV9 (II-6) using polyadenylated RNA extracted Eton the human embryonic lung fibroblast cell line H426 (II-7), the initial sourcs of the growth factor.
Screening of the library (9 x lOs plaques) with 2~~~~~~
the 3zK-labelled 26-mer oligonucleotides identified 88 plaques which hybridized to both pools of oligonucleotide probes.
Characterization and sequencing of selected cDNA clones. Of 10 plaque-purified clones that were analyzed, one, designated clone 49, had a cDNA insert of 3.5 kb, while the rest had inserts ranging from 1.8 kb to 2.1 kb.
Analysis of the smaller clones revealed several common restriction sites. Sequence of a representative small clone, designated clone 32, along with clone 49, demonstrated that they were overlapping cDNAs (Fig II-lA). Overlapping pCEV9 clones 32 and 49, used in sequence determination, are shown above a diagram of the complete structure in which untranslated regions are depicted by a line and the coding sequence is boxed. The hatched region denotes the sequence of the signal peptide and the open region denotes the sequence of the mature protein. Selected restriction sites are indicated. Whereas clone 49 was primed from the poly(A) tail of the message, clone 32 arose during the construction of the library by hybridization of the oligo (dT) primer to an A-rich sequence in the 3' noncoding region of the KGF mRNA.
Description of the seQUence encoding the KGF
olypeptide. Alignment of the two cDNAs (clones 32 and 49) established a continuous sequence of 3.85 kb containing the complete KGF coding sequence (Fig. II-1B). (B) KGF cDNA nucleotide and predicted amino acid sequences. Nucleotides are numbered on the left; amino acids are numbered throughout. The N-terminal peptide sequence derived from purified KGF is underlined. The hydrophobic N-terminal domain is italicized. The potential asparagine-linked glycosylation site is overlined. The variant polydenylation signals, AATTAA
and AATACA, close to the 3~ end of the RNA, are boxed.
An ATG, likely to be an initiation codon was located at nucleotide position 446, establishing a 582-base paid open reading frame that ended at a TAA termination codon at position 1030. This open reading frame 59 a would encode a 194-amino acid polypeptide with a ,~ calculated molecular weight of 22,512 daltons.
The sequence flanking the ATG colon did not conform to the proposed GCC(G/A)CCATGG
consensus for optimal initiation by eukaryotic ribosomes (II-8), however, there was an A three nucleotides upstream of the ATG colon. An A at this position is the most highly conserved nucleotide in the consensus. This ATG colon was preceded 85 nucleotides upstream by a TGA stop colon in the same reading frame.
A 19-amino acid sequence that was homologous to the experimentally determined NHZ-terminus of purified RGF began 32 amino acids downstream of the proposed initiation colon. -There was complete agreement between the predicted and experimentally determined amino acid sequences, where unambiguous assignments could be made.
To search for homology between KGF and any known protein, a computer search of the National Biomedical Research Foundation data base a:ing the FASTP program of Lipman and Pearson was conducted (II-9). Hy this approach, a striking degree of relatedness between the predicted ' primary structure of KGF and those of acidic and basic FGF, as well as the related nst and int-2-encoded proteins was revealed.
Expression of mRNA transcripts of the KGF
ene in human cells. In preliminary attempts to examine expression of KGF mRNA in human cells, a probe spanning the majority of the KGF coding sequence (Probe A, Figure II-lA) detected a single 2.4 kb transcript by Northern blot analysis of total M426 RNA (Fig. II-1C, lane b). Lanes a and c, poly(A)-selected M426 RNA;
lanes b and d, total cellular M426RNA. The filter was hybridized with a 32P-labeled 695 bk Bam/HI/BcI/I
fragment from clone 32 (Probe A, Fig. II-lA). This was considerably shorter than the length of the composite cDNA sequence, 3.86 kb.
However, on screening poly(A)-selected M426 RNA, an additional transcript of approximately 5 kb was detected (Fig. II-iC, lane a). Furthermore, a probe derived from the untranslated region of clone 49, 3' to the end of clone 32 (Probe B, Figure II-lA), hybridized only to the larger message (Fig. II-1C, lane c). Thus, it appears that the KGF gene is transcribed as to alternate RNAs. Two other members of the FGF gene family, bFGF (II-29) and int-2 (II-30), also expresses multiple RNAs, the significance of which remains to be determined.

f r To investigate the normal functional role of KGF, the expression of its transcript in a variety of human celld lines and tissues was examined (Fig. II-3).
Northern blot analysis of KGF mRNA in normal human cell lines and tissues, and comparison with mRNA expression of other growth factors with known activity of epithelia cells was conducted. Total cellular RNAs were isolated by cesium trifluoro-acetate gradient centrifugation. 10 ~,g of RNA were denatured and electrophoresed in 1% formaldehyde gels. Following mild alkali denaturation (50 mM NaOH for 30"), RNA was transferred to nitrocellulose filters using 1 M
ammonium acetate as a convectant. Filters were hybridized to a 32P-labeled cDNA probe containing the 647 bk EcoRI fragment from the 5' end of the KGF coding sequence (A) or similar probes from the other growth factor DNAs. The following human cell types were used:
squamous cell carcinomas (A253, A388 and A431); mammary epithelial cells (85/589); immortalized bronchial epithelial cells (S6 and R1); keratinocytes immortalized with Adl2-SV40; primary human keratinocytes; neonatal foreskin fibroblasts (AG1523);
adult skin fibroblasts (501T); and embryonic lung fibroblasts (WI-39 and M426). As shown in Fig. II-3, the 61 a predominant 2.4 kb KGF transcript was detected ~' each of several stromal fibroblast lines derived from epithelial tissues of embryonic, neonatal and adult sources, but not from epithelial cell lines of normal origin. The transcript was also detected in RNA extracted from normal adult kidneys and organs of the gastrointestinal tract, but not from lung or brain. The striking specificity of RGF RNA expression in stromal cells from epithelial tissues indicated that this factor plays $ normal role in mesenchymal stimulation of epithelial cell growth.
For comparison, the mRNAs of other growth factors with known activity on epithelial cells were also analyzed in the same tissues as listed above. Among the epithelial and stromal cell lines analyzed, there was no consistent pattern of expression of aFGF or bFGF transcripts (gig, II-3). The EGF transcript was not expressed in any of the same cell lines, and was only observed in kidney, among the various tissues. Finally, the TGFa message was not dstected in any o! the stromal fibroblast lines and was expressed at varying levels in each of the epithelial c.ll lines. It was also detected at low levels in kidnay among the tissues examined (Fig. II-3).
_ 2038398 heDa~. Heparin has been shown to substantially increase the mitogenic activity of aFGF for a variety of target cells in culture, and to stabilize it from heat inactivation (II-21, II-22). Despite binding tightly to bFGF, heparin had minimal effects on its mitogenic activity (II-22). In view of the relatedness of KGF to the FGFs, the effect of heparin on KGF mitogenic activity was examined. As shown in Table II-1, thymidine incorporation by HALB/MK cells in response to KGF was inhibited 16 fold when heparin was included in the culture medium. In contrast, the activities of both aFGF and bFGF
were increased by th. same treatment.
production o! anti-KGF antibodies.
Several kinds of antibodies which recognize KGF
or KGF-like polypeptides have been prepared using standard methodologies well known in the art of experimental immunology and summarized in the Methods section, above. These include:
monoclonal antibodies raised in mica against intact, purified protein from human tibroblasts:
polyclonal antibodies raised in rabbits against synthetic peptides with sequences based on amino acid sequences predicted from the KGF cDNA
sequence: polyclonal antibodies raised in rabbits against both naturally secreted RGF from human fibroblasts and recombinant KGF produced in 2~~~~~
E. coli (see next section) .
Monoclonal antibodies from three different hybridomas hays been purified. All three recognize the recombinant as well as the naturally occurring KGF in a solid-phase (ELISA) assay. None cross-reacts with KGF under denaturing conditions (in a Western blot), and none neutralizes mitogenic activity of KGF in the BALB/MK bioassay.
Polyclonal antibodies were generated with a synthetic peptide with the amino acid sequence NDMTPEQMATNVR, corresponding to residues numbered 32 through 44 in KGF (see Fig. II-1).
plus an R (erg) residua instead of the actual asp residua encoded by the cDNA at position 45. The asp residue is probably glycosylated in the natural KGF polypeptide and, therefore, appeared to be an erg in the amino acid sequencing data obtained directly from that polypeptide (see Discussion, below). Polyclonal antibodies generated with this aynthstic peptide recognize both naturally occurring and recombinant KGF in ELISA and Western blot analyses at a level of sensitivity of at least as low as 10 ng protein.
These antibodies, however, do not neutralize mitogenic activity of KGF in the BALB/MK
bioassay.

Polyclonal antisera against intact natural KGF protein recognizes KGF in both ELISA ~ ~ ~~ !~ ~ ,~
and Western blot assays. Such antibodies also appear to inhibit mitogenic activity of KGF in the BALB/MK bioassay.
------~ ~~ of KGF cDNA in . coli. KGF
r cuLCaai.vaa cDNA Was expressed to produce polypeptide in B.coli by placing its coding sequence under control of the hybrid ~ promoter (comprising elements of fig and a~.,~c promoters) , in the plasmid pKK233-2 (II-31). To accomplish this, a specific length of KGF cDNA that contained the information to code for the mature KGF molecule (i.e., without ita signal peptide) waa amplified using the polymerase chain reaction technique (II-32). The fragment was directionally inserted between two sites in the vector, namely the Ncol site, made blunt ended by S1 nuclease digestion, and the XindIII site, using standard recombinant DNA
methodology. Selected recombinants were sequenced at their cDNA 5' ends to ensure correct alignment of the ATG initiation colon with the regulatory elements of th~ ~ promoter.
Several recombinants were tested for protein production by the usual gall scale methods. In bri~t, the clones were grown to mid-exponential phas~ (ODD '0.5), treated with 1 mM

2~3~3~8 isopropyl ~-D-thiogalactopyranosi (IPTG) for 90 minutes, and cell extracts were run on SDS-polyacrylamide gels for Western blot analysis. All recombinants tested synthesized a protein that was recognized by antibodies raised against an amino-terminal KGF peptide. One recombinant was selected which showed the greatest induction from IPTG, for further protein analyses.
One liter of bacteria was grown up in NZY
l0 broth containing 50 ~g/ml ampicillin and 12.5 ~g/ml tetracycline, to ODs95-0.5, and treated for 90 min with IPTG. The cells were collected by centrifugation, resuspended in 50 mM sodium phosphate (pH 7.3), 0.2 M
NaCl, and lysed by sonication. Cell debris was removed by centrifugation, and lysate applied directly to a heparin-Sepharose affinity column.
As determine by Western blot analysis and mitogenic activity in keratinocytes, recombinant KGF
was eluted in 0.5 - 0.6 M NaCl. Subsequent purification of the HSAC material with a Mono-S (FPLC) column (Pharmacia) yielded a preparation of KGF
estimated to be >_90% pure, as judged by electrophoretic analysis using SDS-polyacrylamide gels and silver-staining.
The following specific test was carried out to determine the estimated molecular weight size of the non-glycosylated bacterially expressed recombinant KGF
protein that is represented in the Fig. II-4.
Fig. II-4A represents the Mono-S
chromatography pattern of heparin-Sepharose purified, non-glycosylated KGF. The mitogenic activity (~ - ~ - ~) coincides with elution of protein peaks, as indicated by optical absorbance read at 280 nm ( ) Fig. II-4B shows the SDS-PAGE analysis of mitogenically active fractions from KGF preparation.
Silver-stain of 14% polyacrylamide gel demonstrates purification of major active species at or slightly retarded relative to the 21.5 kD molecular weight marker, as well as minor species (here appearing as a doublet) with apparent molecular weight of approximately 16 kD. Lane 1: crude lysate; lanes 2 and 3: peak fractions from heparin-Sepharose chromatography; lanes 4-9: fractions 26-31 from Mono-S
chromatography shown in Figure II-4A.
Fig. II-4C shows the immunoblot analysis of selected fractions from the Mono-S-chromatography. The purified proteins in mitogenically active Mono-S
fractions cross-react with a rabbit neutralizing polyclonal antiserum raised against highly purified human KGF. Lanes 1-6 correspond to fractions 26-31 from Mono-S-chromatography shown in Fig. II-4A and silver-stained lanes 4-9 shown in Fig. II-4B.
66a Recombinant KGF efficiently stimulated thymidine incorporation into BALB/MK keratinocyte cells, but was only marginally active on NIH/3T3 66 b fibroblasts. Half-maximal stimulation of the HALB/MK cells in the standard keratinocyte bioassay was achieved with a concentration of between 2 to 5 ng/ml, compared to a concentration of 10 to 15 ng/ml for KGF purified from M426 cells. one liter of bacterial cells yielded approximately 50 ~g of Mono-S purified recombinant KGF.
Construction of a~chimera containinct KGF
and aFGF sequences. The s~udies above indicated that KGF possessed two distinctive characteristics which might be encoded by distinct portions or domains of the polypeptide sequence, as is well known to occur in coding sequences of other multifunctional polypeptides.
To test this possibility, a chimeric DNA segment encoding the NHZ-terminal sequence of KGF grafted onto the C-terminal core of aFGF was constructed, as follows. A Sau3AI restriction enzyme site (GATC) in the 5' end of the KGF cDNA, within codons for residues 78 and 79 (erg and ile, respectively: see Fig. II-1) was cut and joined to an homologous site in the aFGF cDNA within codons for amino acids 39 (erg) and 40. The 3' and 5' ends of this chimeric DNA were joined to the vector DNA of the plasmid pIQt233-2 by the same method used for insertion of the KGF cDNA

encoding the secreted form of polypeptide (see ~~e~O~.~~
Methods, above).
When recombinant E. coli cells were constructed using the vector carrying the chimera, and expressions tests were conducted as described for mature KGF, above, a novel product with properties of both RGF and aFGF was produced. The peptids was enriched by heparin-Sepharose chromatography and found to have a target cell preference for keratinocytes, like RGF, with minimal activity on fibroblasts (NIH/3T3). The mitogenic activity of this chimeric polypeptide lacks, however, susceptibility to inhibition by heparin, a characteristic which parallels that of aFGF
rather than RGF. In fact, the mitogsnic activity on keratinocytes is actually enhanced by heparin, as is the case for aFGF. Thus the peptide domains responsible for cell target specificity and heparin sensitivity are clearly distinct and readily separable in RGF, according to the practice of the present invention.
The experiments described in this section illustrate the practice of several principal embodiments of the present invention.
These include isolation of cDNAs encoding RGF, expression of such cDNAs in recombinant cells, production of various antibodies reactive with KGF, and construction and expression of a chimeric cDNA encoding a novel growth factor with amino acid sequences and related functionalities of both KGF and aFGF. The following points related to these embodiments may also be noted to enhance the understanding of the present invention.
The sequence predicted from the KGF cDNA
agreed with the amino acid sequence determined from the purified KGF form secreted by human fibroblasts. Moreover, the sequence offered potential explanations for positions where definitive amino acid assignments could not be made by direct amino acid sequencing. Residues 32 and 46 are predicted from the cDNA sequence to be cysteines, and hydrolyzed derivatives of unmodified cysteins residues are not detectable following Edman degradation. The~predicted KGF
amino acid sequence also contained one potential N-linked glycosylation site (Asn-X-Ser/Thr) from residues 45 through 47. If Asn 45 were glycosylated, it would not be detected by the amino acid sequencing methods employed here. In fact, KGF migrates as a broad band on NaDodS04/PAGE at a higher molecular weight than predicted for the purified protein. This may be accounted for by glycosylation.
The FGFs are heparin-binding mitogens with broad target cell specificities (II-10).
The hst gene was identified as a transforming gene from a human stomach tumor (II-li), adjacent normal stomach tissue (II-12), and from Kaposi's sarcoma (II-13), by standard NIH/3T3 transfection assays. Ths product of the i~t-1 gene is expressed normally during mouse embryogenesis (II-14) and aberrantly after proviral integration of mouse mammary tumor virus (II-15).
KGF is the sixth member of the fibroblast growth factor family to be identified (II-28). While the name FGF-6 does not seem suitable because RGF is devoid o! activity on fibroblasts, this nomenclature may also be used for this growth factor, to denote its structural relationship to the FGF family. ~s all previously characterized growth factors either exclude epithelial cells as targets or include then among a number of sensitive target cells, the highly specific nature of KGF mitogenic activity !or epithelial cells, and the sensitivity of keratinocytss in particular, make it unique.

In studies to date, expression of the '"~' KGF transcript appears to be specific for stroma~
cells derived from epithelial tissues, suggesting its function in normal epithelial cell proliferation. The availability of the KGF cDNA
clone will make it possible to determine whether abnormal expression of this growth factor can be implicated in clinical conditions characterized by epithelial cell dysplasia and/or neoplasia.
Moreover, the ability to produce large quantities of this novel growth factor by recombinant techniques should allow testing of its clinical applicability in situations where specific growth of epithelial cells is of particular importance.
Alignment of the RGF sequence with the five other proteins of the FGF family revealed two major regions of homology, spanning amino acids 65-156 and 162-189 in the predicted KGF
sequence, which ware separated by a short, nonhomologous series of amino acids with varying lengths in different members of tha family (Fig.
II-2) . In the case of int-1, the length of this sequence was 17 residues, while in hs~, the two homologous regions were contiguous. In KGF the intervening sequence consisted of five amino acids.

In the aligned regions, the KGF amino ,~ acid sequence was about 44~ identical to int-1 (mouse), 39~ identical to bFGF (human), 37~
identical to aFGF (human) and 33~ identical to hsr (human). In this same region, all six proteins were identical at 193 of the residues, and allowing for conservative substitutions, they showed 28~ homology.
As shown in Fig. II-2, the amino termini of these related proteins are nonhomologous and of variable length. The primary ItGF and lrst translation products contain hydrophobic N-tenainal regions which likely serve as signal sequences (II-16). The fact that this N-terminal domain is not present in the mature RGF molecule (Fig. II-iB) further supports this conclusion.
In contrast, the FGFs are synthesized apparently without signal peptides (II-10) . The int-1 protein contains an atypically short region of N-terminal hydrophobic residues (II-17), but it is not known if the protein is secreted. Moreover, the int-1 protein contains a long C- terminal extension compared to the other family members.
Purified KGF contains five cystsine residues, two of which are conserved throughout the family of FGF related proteins (Fig. II-2).
Also of note are the five pairs of basic residues throughout the KGF sequence. This same pattern has been observed in other FGF family members and ~ '~ ~' ~
,...~..-_ may be involved in their interaction with heparin (II-18). Dibasic sites are also common targets for proteolytic processing and such processing might account for the microheterogeneity observed in some RGF preparations (unpublished dataj.
The KGF cDNA sequenc~ was AT rich throughout its length, but particularly so in the 3' untranslated region where the AT content was 70~ as compared to 60~ in the putative coding sequence and 63~ in the 5' untranslated region.
The 3~ untranslated region contained a large number of ATTTA sequences, which have been proposed to be involved in the selected degradation of transiently expressed, unstable RNAs (II-19). There was no classical AATAAA
polyadenylation signal but two variant sequences, AATTAA and AATACA (II-20), wer~ detected 24 and 19 nucleotides, respectively, upstream of the poly(A) sequence at the 3~ end of th~ cDNA.
It has be~n suggested that the heparin effect on acidic FGF is either dum to stabilization of the active conformation of thm growth factor or to formation of a tertiary complex with acidic FGF and its receptor (II-21, II-22). If so, heparin may stabilize a conformation of KGF that is not as active as the free molecule, or form a tight complex that is ,,~ unable to efficiently interact with its receptor.
While its ability to bind heparin reflects the structural similarities of KGF with the FGF's, the differences in target cell specificities between these related mitogens is remarkable. The FGF's induce division of most nonterminally differentiated cells of both embryonic mesodenaal and neurosctodermal origin.
In addition to fibroblasts and vascular endothelial tissues, mesodermally derived targets in culture include myoblasts, chondrocytes and osteoblasts (II-23). FGF's era also mitogenic for glial astrocytes and nsuroblasts (II-24).
The product of the oncogene isolated from Raposi's sarcoma, which is identical to kst, also stimulates proliferation of NIH/3T3 and capillary endothelial cells (II-25). To date, KGF induced mitogenesis has only been observed in epithelial cells, and the absence of any detectable activity in fibroblasts or endothelial cells has also been demonstrated (see Experimental Section I, above and II-3). It seems likely, therefore, that KGF
acts through a different cell surface receptor than the FGFs.
There is no significant N-terminal homology between KGF and other FGF-related proteins. Thus, the construction of chimeric molecules between KGF and a prototype FGF was undertaken to determine whether the KGF
N-terminal domain is sufficient to account for its unique target call specificity. Tha results on the first such recombinant polypeptide sequence indicate that the N-terminal domain of KGF essentially encodes the cell preference for keratinocytes, while the susceptibility of KGF to heparin is encoded somewhere in the C-terminal core region which was replaced by sequences of aFGF. This novel KGF-like growth factor may have advantages in clinical applications where administration of an epithelial-specific growth factor is desirable in th~ presence of heparin, a commonly used anticoagulant. Additional studies on chimeras should also provide insights into which specific domains in the C-terminal cots contribute the different eft~cts of heparin on their biologic activities.
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II-1. James, R. and Bradshaw, R. A. (1984) Ann. Rer. Bixleern. 53, 259-292.
II-2. Spore, M. 8. and Todaro, G. J. (1980) N.
End. !. Med. 303, 878-880.
iI-3. Rubin, J. S., Osada, H., Finch, P. W., Taylor, W. G., Rudikoff, S. and 7s Aaronson, S . A. ( 1989 ) Proc. Natl. Acad.
Sci.

w'"' USA (in press) .

II-4 . Crick, F. H. C. ( 1966) J. Mol. Biol. 19, 548-555.

II-5. Ohtsuka, E., Matsuki, S., Ikehara, M., Takashi, Y. and Matsubara, R. (1985) J.

Biol. Chtm. Z60, 2605-2608.

II-6. Miki, T., Matsui, T., Heidaran, M. and Aaronson, S . A. ( 1989 ) Proc. Nail. Acad.
Sci.

(in press).

II-7. Aaronson, S. A. and Todaro, G. J. (1968) Virology 36, 254-261.

II-8. ~ Kozak, M. (1987) Nercl. Acids Res. i3, 8125-8148.

II-9. Lipman, D. J. and Pearson, R. W. (1985) Scienct ZZ9, 1435-1441.

II-10. Thomas, R. (1987) FASEB J. i, 434-440.

II-11. Taira, M., Yoahida, T., Miyagawa, R., Sakamoto, H., Terada, M. and Sugimara, 2 0 T . ( 19 8 7 ) Proc. Notl. Aced . Sci. USA
8 4 , 2980-2984.

II-12. Yoshida, T., Miyagawa, R., Odagiri, H., Sakamoto, H., Little, P. F. R., Terada, M. and Sugimara, T. (1987) Proc. Narl. Acad.

Sci. USA S4, 7305-7309.

II-13. Dells-Bovi, P. and Basillico, C. (1987) Proc. Notl. Acad. Sci. USA S4, 5660-5664.

II-14. Jakobovits, A., Shackleford, G. M.

, ~ ~C~~a~
Varmus, H. E. and Martin, G. R. ( 198 ~ ~ ~ ~3 Proc. Natl. .lcad. Sci. US.! 83, 7806-7810.

II-15. Peters, G., Brookes, S. and Dickson, S.

(1983) Ctll 33, 364-377.

II-16. von Heijne, G. (1986) Nucl. Acids Rts. 14, 4683-4690.

. II-17. Moors, R. Casey, G., 8rookes, S., Dixon, M., Peters, G. and Dickson, C. (1986) EMBO J. S, 919-924.

. II-18. Schwarzbauer, J.E., Tamkum, J.M., Lemischka, I. R. and Hynes, R. O. (1983) Cell 35, 421-431.

II-19. Shaw, G. and Ramen, R. (1986) Cell 46, 659-667.

II-20. Birnsteil, M. L., Busalinger, M. and Strub, R. (1985) Ctll 41, 349-359.

II-21. Schriaber, A. B., Ranny, J., Kowalski, w W., Friesel, J., Mehlman, T. and Maciag, 2 0 T. ( 198 5 ) Proc Natl. Acad. Sci. USA 8Z , 6138-6142.

II-22. Gospodarowizc, O. and Chsng, J. (1986) !. Cell Physiol. 1s8, 475-485.

II-Z3. Thomas, R. A. and Giminaz-Gallego, G.

(1986) Trtnds Biochtm. Soc. il, 81-84.

II-24. Gensburger, C., Labourdette, G. and Sensembrenner, M. (1987) FEES Lttt. Z17, 1-5.

II-25. Delli-Bovi, P, Curatola, A M., Kern, F.

G., Greco, A., Ittman, M. and Basilico, C. (1987) Ctll 50, 729-737.

II-26. Sanger, F., Nicklen, S. and Coulson, A.

R. (1977) Proc. Natl. Acad. Sci. 1ISA 74, 5463 5467.

II-27. Yanisch-Perron, C., Vieira, J. and Messing, J. (1985) Gene 33, 103-119.

II-28. Zhan, X., Bates, B., Hu, X. and Goldtarb, M. (1988) Mol. Cell. Biol. e, 3487-3495.

II-29. Abrahams J. A., Mergia, A., Whang, J.L., .

Tumolo, A., Friedman, J., Hjerrild, R.

A., Gospodarowicz, D. and Fiddea, J. C.

(1986) Science Z33, 545-548.

II-30. Mansour, S. L. and Martin, G. R. (1988) EMBO J. l, 2035-2041.

II-31. Amman, E. and Brosius, J. (1985) Gent 40, 183.
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(1988) Science Z41, 1346-1349.

2fl~~~9~
EBPERIMENTAh SECTION III
MATERIALS AND METHODS
Human keratinocyte culture Cultures of human epidermal cells were derived from full-thickness biopsies of neonatal foreskin as previously described (III-26). For proliferation assays in serum-free medium, secondary cultures were transferred to 60 mm petri dishes or 24 well cluster plates (Falcon) in standard medium consisting of MCDB
153, supplemented with 0.03 mM Ca2+,0.4 ~,g/ml hydrocortisone, 5 ~g/ml insulin, 0.1 mM ethanolamine, and 70 ~g/ml whole bovine pituitary extract (wBPE) (Clonetics Corp. San Diego, CA). Cell adhesion was achieved by precoating petri dishes sequentially with polyl-D-lysine (10 ~,g/cm2), and human fibronectin (1 ~,g/cm2) for 30 min at 37°C (III-16). Medium was changed every 2 days.
EGF and TGFa were obtained from Collaborative Research and Bacham, Inc. (Torrance, CA), respectively.
Preliminary experiments were performed with KGF
purified from culture fluids of human embryonic fibroblasts as previously described (III-29). Most of the studies described were performed with recombinant KGF expressed in Escherichia coli as described in 2~~~~9~
Experimental Section II. Recombinant KGF was purified by heparin Sepharose chromatography and was at least 90% pure as assessed by SDS-PAGE analysis. With subsequent purification using Mono-S ion exchange chromatography, it was possible to show that all activity on keratinocytes was due to KGF.
To measure cell number, cultures were harvested by incubation in 0.5% trypsin-EDTA (0.2%) solution for 15 min at 37°C, and cell counts were performed by haemocytometer.
Antis~ra Rabbit anti-human keratin 1 (anti K1) (III-8, 11 and 12) was provided by S. Yuspa, Laboratory of Cellular Carcinogenesis and Tumor Promotion, NCI. This affinity purified antibody was used at a 1:1,000 dilution. Mouse anti-human filaggrin was purchased from BTI, Biomedical Technologies, Inc., (Stoughton, MA), and used at a 1:500 dilution.
Immunoblotting Cell lysates were prepared by scraping cells from 100 mm petri dishes into OFLB lysis buffer (10.M urea, 2% NP-40, 100 mM dithiothreitol, 1 mM sodium vanadate, 1.6% LKB ampholine pH 5-7, and 0.4% LKB ampholine pH 3-10). Protein measurements were made by the Bradford 2~3~~98 procedure (Bio-Rad Laboratories, Richmond, CA) using thyroglobulin as a standard (III-3). Approximately 100 ~g protein was loaded in each lane and resolved in 8%
polyacrylamide-SDS gels (III-20).
Protein bands were electrophoretically transferred to Immobilon-PVDF membranes (Millipore) in 25 mM Tris, pH 8.3, 192 mM glycine, 20% methanol for 90 min at 1 Amp. PVDF membrane blots were processed with the relevant antisera and l2sl_labeled protein A as described in (III-10).

Morphology of human keratinocytes cultur~d in RGF
In order to examine the effects of KGF on human keratinocytes, serum-free medium (Clonetics) was supplemented with 70 ~g/ml whole bovine pituitary gland extract (wBPE). In addition, petri dishes were precoated with polylysine and human fibronectin as previously described (III-6,34). Under these conditions, secondary human keratinocytes attached, spread well, but grew very slowly. Figure III-1 A, B
and C show a typical culture 4 days after plating in this medium. Panel A was a control. In Panel B, cultures exposed to 10 ng/ml of recombinant human KGF
demonstrate an obvious increase in cell proliferation.

~0~ ~~~8 For comparison, the effects of 10 ng/ml of EGF under the same conditions were examined. As shown in Figure III-1C EGF-exposed cultures also showed increased keratinocyte proliferation. With EGF, there was also an obvious outgrowth both of fibroblasts and melanocytes that was not observed in KGF-stimulated cultures with repeated cell passage.
Comparison of proliferation of human keratinocytea in rssponss to RGF and EGF
In order to quantitatively compare the effects of KGF and EGF on human keratinocyte proliferation, the dose response to each growth factor was investigated. Cells were seeded at a density of 104 cells per well in a 24 well cluster plate and grown for 4 days in standard low Ca2+ medium supplemented with,varying KGF or EGF concentration. Experiments were performed in triplicate, and results represent mean values ~ standard deviations. In a 4-day assay, both growth factors induced significant increases in cell proliferation, ranging from approximately 4.5-fold under optimal conditions with EGF (O) to 6-fold in response to KGF (~) (Fig. III-2). Moreover, both growth factors significantly increased proliferation at low concentration. For example, there was a greater than two-fold increase in cell number with as little as 0.1 ng/ml of KGF.
No significant additive effects on proliferation were observed using 10 ng/ml of EGF in combination with 10 ng/ml KGF. The kinetics of keratinocyte proliferation in response to the two growth factors are shown in Figure III-3. Following a short lag phase, KGF (10 ng/ml,~) and EGF (10 ng/ml,o) induced proliferation with similar kinetics. In contrast, untreated cultures (e) grew more slowly and plateaued in their proliferation after 10 days, while the growth factor-stimulated cultures continued to increase in cell number. Thus, under these experimental conditions, KGF was at least as effective as EGF for inducing proliferation of human keratinocytes.
The effects of KGF on colony formation by keratinocytes plated at low cell density were determined. Colony formation was significantly increased both in number and size over that observed in control cultures. Moreover, colonies in KGF-treated cultures showed a relative abundance of small cells that have been previously shown to represent less well differentiated keratinocytes (III-26). A similar pattern was observed for colonies that formed in EGF-treated cultures.

2~er~~e9~~
,.-Effects of th~ calcium-induced differentiation signal on RGF- and EGF-induced keratinocyte proliferation It has been reported that keratinocytes in culture differentiate in response to calcium concentrations greater than 0.15 mM (III-2,16,18).
The effects of increasing calcium concentration on proliferation and differentiation in response to KGF
were determined. Cells were plated at a density of 104 cells per well in a 24 well cluster plate and grown for 4 days in standard medium alone (e) or supplemented with 10 ng/ml of either KGF (~) or EGF (O).
Experiments were performed in triplicate, and results represent mean values ~ standard deviations. As shown in Figure III-4, the peak of cell proliferation in response to KGF (10 ng/ml) was observed at 0.05 mM, and Ca2+ concentrations of 0.1 and 0.5 mM were associated with increased cell proliferation as well. At 1.0 mM
Ca2+, there was no significant net increase in cell number in a 4-day assay. The results with EGF (l0 ng/ml) were essentially the same (Fig. III-4). By use of colony formation as a measure of keratinocyte proliferation, it was reported (III-38) that the peak of cell growth was at 0.3 mM Ca+ with 10 ng/ml EGF.
They also observed the above noted decreased growth at higher Ca+ concentrations. Thus, neither KGF nor EGF

was able to block the inhibitory effects of high Ca2+
on keratinocyte proliferation.
The morphologic effects of high Caz+ were readily detectable as well. In control as well as growth factor-treated cultures, cells appeared more flattened, less refractile, and cell borders became less distinct. However, the effects were less dramatic in either of the growth factor-supplemented cultures (Fig. III-5). Cells were maintained for 4 days either in standard medium plus 1.0 mM Ca2+ (control) or supplemented with KGF (10 ng/ml) or EGF (10 ng/ml).
Calcium-dependent expression of differ~atiation markers Epidermal differentiation is characterized by a number of morphological and biochemical changes that result in the development of stratified squamous epithelium (III-9,14,28). In the course of differentiation, there is a sequential expression of specific markers. Among such markers, keratins including K1 (67 kd) and K10 (59 kd), are expressed early in the differentiation program as cells begin their maturation in the basal or first suprabasal layer (III-12,14,23,28,31). Filaggrin (III-17,21) is expressed later as cells enter the granular cell layer (III-9). Expression of keratins and filaggrin genes is presumably regulated as a function of the stage of 2~~~~~~8 -keratinocyte differentiation by various external agents such as calcium and growth factors (III-27,32,37,39).
To examine the effects of the growth factor on these markers of differentiation, KGF- or EGF-stimulated cultures were exposed to increasing calcium concentration for 6 days. Human keratinocyte cultures were maintained for 6 days in the presence of varying Ca2+ concentrations as indicated, except for the last group which was exposed to 1.0 mM Ca2+ for 18 hr.
Cells were maintained either in standard medium (control) or supplemented with KGF (10 ng/ml) or EGF
(10 ng/ml). Cells were lysed, processed and immunoblotting was performed with anti-keratin 1 or anti-filaggrin sera, as described in the "Materials and Methods". As shown in Figure III-6, immunoblots prepared from cell lysates under different treatment conditions were probed with the human anti-K1 antibody.
At 0.03 mM Ca2+, a K1 reactive protein species migrating at 67 kd was observed in untreated keratinocytes, while neither KGF- nor EGF- exposed cultures showed this protein. At 0.15 mM Ca2+, the 67 kd species was increased in control cultures and could be seen at lower level in KGF but not in EGF-supplemented cultures. This same pattern was observed at 1 mM Ca2+. Because the appearance of K1 is known to be time-dependent (III-39), cultures treated with 1.0 ._ 2~~~~~~
mM Ca2+ for 18 hr were examined. The K1-reactive protein was readily detectable in control cultures and was observed at lower levels with KGF but not EGF
exposure (Fig. III-6). This indicates that human keratinocytes were rendered significantly more resistant by EGF than KGF to the appearance of this early differentiation marker in response to the calcium signal.
Filaggrin is synthesized as a 400 kd l0 precursor, which is sequentially processed to a final product of around 39 kd (III-17,21). Figure III-6 shows that in human skin, multiple intensely staining filaggrin immunoreactive proteins were detectable. In contrast, these proteins were not observed in secondary human keratinocyte cultures at low Ca2+ in the presence or absence of either growth factor. At increasing calcium concentration, control cultures demonstrated multiple bands similar to the pattern observed in skin.
As was the case with K1, the appearance of filaggrin was specifically inhibited in cultures exposed to EGF
but not KGF (Fig. III-6). At 18 hr, there also appeared to be relatively less induction of filaggrin relative to K1 (Fig. III-6), consistent with the known kinetics of appearance of these markers.
To further explore the differential effects of KGF and EGF on the appearance of biochemical markers .. 2~~~~~~
of terminal differentiation, we examined the Caz+
response of keratinocytes propagated in TGFa, which like EGF, interacts with the EGF receptor (III-5,33).
Human keratinocyte cultures were maintained for 6 days with 0.03 or 1.0 mM Ca2+ in standard medium (control) or supplemented with 10 ng/ml of KGF or TGFa. Cells were lysed, processed and immunoblotting analysis was performed with anti-keratin 1 serum as described in the "Materials and Methods". The appearance of Kl was blocked similarly in response to TGFa and EGF, while the marker was induced despite the presence of KGF
(Fig. III-7). This indicates very different patterns of biochemical markers induced in response to the Ca2+
differentiation signal in keratinocytes stimulated by KGF as opposed to members of the EGF family.

Keratinocyte growth factor (KGF) is identified hereinabove as a human epithelial-specific growth factor. The growth factor is released in culture by stromal cells derived from major epithelial organs including skin and gastrointestinal tract. "In vivo~', the KGF transcript is in stromal cells of these same normal tissues. This demonstrates that KGF plays an important role in normal epithelial cell proliferation (III-13). The results of this .,..
Experimental Section further demonstrate that KGF acts as a potent mitogen for human keratinocytes in culture, equivalent to or more active on a molar basis than EGF.
In the sequential program of keratinocyte differentiation, the basal cell at the innermost layer of the epidermis is the stem cell. Progeny cells migrate into the upper epidermal layers, ultimately terminally differentiating to form the stratum corneum (III-37). During this process, the cells change dramatically both morphologically and biochemically.
Thus, one could expect that growth factors of physiologic importance for epidermal cells would be able to sustain proliferation of undifferentiated basal cells and yet not interfere with proper signals for terminal differentiation. According to this Experimental Section, the results indicate that in response to the differentiation signal induced by a high calcium concentration, KGF-treated keratinocytes ceased to proliferate. The cells developed morphologic features of terminally differentiated keratinocytes as well. Finally, under conditions of high calcium exposure, KGF-stimulated human keratinocytes expressed both K1 and filaggrin, early and late markers, respectively, of keratinocyte differentiation.
In contrast to these results with KGF, the well-characterized EGF molecule was able to block or at ..~ 2~~~~~~8 least significantly retard expression of biochemical markers of keratinocyte differentiation under identical conditions of high calcium exposure. Thus, both K1 and filaggrin were either not detectable or were much reduced in expression in EGF-treated keratinocytes.
EGF, itself, is not thought to be normally expressed in epidermal or dermal cells. In contrast, there is evidence that TGFa, which also binds and activates the EGF receptor, is expressed in certain epithelial cells including keratinocytes (III-6,15). This section demonstrates that TGFa, like EGF, blocked the appearance of K1 in response to the calcium differentiation signal. The results of this section demonstrate potentially significant differences in the abilities of specific epithelial growth factors to modulate differentiation responses to normal physiologic stimuli "in vivo".
There is most likely a molecular basis for the differing responses to the calcium signal in keratinocytes exposed to KGF as opposed to either EGF
or TGFa. EGF may interfere with the normal calcium-induced response by blocking some critical intracellular signalling pathway. There is accumulating evidence that the ability of keratinocytes to respond to the calcium signal is dependent at least in part upon the substratum that they produce (III-1).

~.., ~ ~ ~ ~? rf Production of substances such as fibronectin, laminin, or collagen in response to KGF or EGF could significantly differ which may explain the patterns observed.
Considerable attention has been focused on the potential clinical application of growth factors to wound healing (III-4,22) and tissue repair. In particular, epithelium derived from keratinocytes cultured on feeder layers has been successfully applied to such clinical conditions as burns (III-7,19) and chronic ulcers (III-25). The ability to speed wound repair by direct application of growth factors to the wound site has been tested experimentally with a variety of such factors. EGF and TGFa have been reported to stimulate regeneration of epithelium (III-4,24,30), but in one study differentiation abnormalities were reported as well (III-22). This section in determining that KGF is associated with a normal differentiation response in vitro demonstrates its clinical application for epithelial cell regeneration and repair.
REFERENCEB FOR EXPERIMENTAL III
III-1 Adams, J.C., and Watt F.M. (1989) Nature, 340:307-309.

s~ l! si ~~,~
V' ~ !~ ed r~
,,.
III-2 Boyce, S.T., and Ham, R.G. (1983) J. Invest.
Dermatol. ~Suppl.J, 81:33-40.
III-3 Bradford, M.M. (1976) Anal. Biochem., 86:248-254.
III-4 Brown, G.L., Curtsinger, L., Brightwell, G.L., Ackerman, D.M., Tobin, G.R., Polk, H.C., Na~scimento, C.G., Valenzuela, P., and Schultz, G.S. (1986) J. Exp. Med., 163:1319-1324.
III-5 Carpenter, G., Stoscheck, C.M., Preston, Y.A., and DeLarco, J.E. (1983) Proc. Natl.
Acad. Sci. U.S.A., 80:5627-5630.
III-6 Coffey, R.J. Jr., Derynck, R., Wilcox, N.J., Bringman, T.S., Goustin, A.S., Moses, H.L., and Pittelkow, M.R. (1987) Nature, 328:817-820.
III-7 Compton, C.C., Gill, J.M., Bradford, D.A., Regauer, S., Gallico, G.G., and O~Connor, N.E. (1989) Lab. Invest., 6(5):600-612.
III-8 Dale, B.A., Holbrook, K.A., and Steinert, P.M. (1978) Nature, 27:729-731.
III-9 Dale, B.A., Resing, K.A., and Lonsdale-Eccles, J.D. (1985) Annals of the New York Academy of Sciences, New York, Vol. 455, pp.
330-342.

~ ~

III-10 ., Di Marco, E.D., Pierce, J.H., Fleming, Kraus, M.H., Molloy, C.J., Aaronson, S.A., and Di Fiore, P.P. (1989) Oncogene, 4:831-838.

III-11 Eichner, R., Bonitz, P., and Sun, T.T. (1984) J. Cell. Biol., 9:1388-1396.

III-12 Eichner, R., Sun, T.T., and Aebi, U. (1986) J. Cell Biol., 102:1767-1777.

III-13 Finch, P.W., Rubin, J.S., Miki, T., Ron, D., and Aaronson, S.A. (1989) Science, 245:752-755.

III-14 Fuchs, E., and Green, H. (1980) Cell, 19:1033-1042.

III-15 Gottlieb, A.B., Chang, C.K., Posnett, D.N., Faneli, B., and Tam, J.P. (1988) J. Exp.

Med., 167:670-675.

III-16 Ham, R.G. (1982) Cold Spring Harbor Laboratory, pp. 39-60.

III-17 Harding, C.R., and Scott, I.R. (1983) J. Mol.

Biol., 170:651-673.

III-18 Hennings, H., Michael, D., Cheng, C., Steinert, P., Holbrook, K., and Yuspa, S.H.

(1980) Cell, 19:245-254.

III-19 Herzog, S.R., Meyer, A., Woodley, D., and Peterson, H.D. (1988) J. Trauma, 28:195-198.

III-20 Laemmli, U.K. (1970) Nature, 227:680-685.

2 ~i :~ ~~ , ~ ~:
~'~r~~t.~u III-21 Lonsdale-Eccles, J.D., Resing, K.A., Meek, R.L., and Dale, B.A. (1984) Biochemistry, 23:1239-1245.
III-22 Lynch, S.E., Colvin, R.B., and Antoniades, H.N. (1989) J. CZin. Invest., 84:640-646.
III-23 Molloy, C.J., and Laskin, J.D. (1988) Differentiation, 37:86-97.
III-24 Niall, M., Ryan, G.G., and O'Brien, B.McC.
(1982) J. Surg. Res., 33:164-169.
III-25 Pye, R.J. (1988) Eye, 2:172-178.
III-26 Rheinwald, J.G., and Green, H. (1977) Nature, 265:421-424.
III-27 Rice, R.H., and Green, H. (1979) Cell, 18:681-694.
III-28 Roop, D.R., Hiutfeldt, H., Kilkenny, A., and Yuspa, S.H. (1987) Differentiation, 35:143-150.
III-29 Rubin, J.S., Osada, H., Finch, P.W., Taylor, W.G., Rudikoff, S., and Aaronson, S.A. (1989) Proc. Natl. Acad. Sci. U.S.A., 86:802-806.
III-30 Schultz, G.S., White, M., Mitchell, R., Brown, G., Lynch, J., Twardzik, D.R., and Todaro, G.J. (1987) Science, 235:350-352.
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Invest. Dermatol., 75:183-186.

*.
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EBPERIMENTAL SECTION I0 2 p 3 g 3 9 8 MATERIALS AND METHODS
Recombinant KGF was purified as described in Experimental Section II. Acidic and basic FGF purified from bovine brain and their lzsl-labelled derivatives (150-200 ~cCi/~g) were obtained from R & D Systems.
Affinity-purified antiphosphotyrosine (aP-Tyr) was prepared as described by White and Kahn (IV-11).
Heparin-Sepharose CL-6B was purchased from Pharmacia LKB Biotechnology Inc. GammmaBind-G agarose*was obtained from Genex, Izsl-Labelled sodium (>5000 Ci/mM) was purchased from Amersham Corp. Recrystallized bovine serum albumin (BSA) was obtained from ICN.
Disuccinimidyl suberate, Triton X-100; BCA protein assay reagent, and dithiothreitol were purchased from Pierce Chemical Co. Heparin, aprotinin, and phenylmethylsulfonyl fluoride were obtained from Sigma.
Tissue culture plasticware was obtained from Costar.
Reagents for SDS-PAGE were purchased from Bio-Rad.
Iodination of KGF. Recombinant KGF was radiolabelled with 'zsI_labelled sodium by the chloramine-T method (IV-12). KGF (3 ~g/50 ~l in 20 mM
phosphate buffer + 1.0 M NaCl,pH 7.4) was reacted with chloramine-T (1.2 ~,g/4 ~,1 of phosphate buffer) and lzsI-labelled sodium (1 mCi/10 ~1) at 24°C for 1 min. The * trade mark reaction was terminated by the addition of sodium metabisulfite (10 ~g/8 ~1), and the mixture was then diluted with phosphate buffer + 0.1% BSA (200 ~1) and applied to a column (300 ~1 packed volume) of heparin-Sepharose CL-6B preequilibrated in phosphate-buffered saline + 0.1% BSA (PBS/BSA). The sample was recycled several times, and the column was washed with 30m1 of PBS/BSA. Elution with aliquots (200 ~1) of PBS/BSA +
0.85M NaCl removed >98% of trichloroacetic acid-precipitable radioactivity from the column. Peak fractions (specific activity, 150-250 ~Ci/~g) were >99%
trichloroacetic acid precipitable, migrated as a single band on SDS-PAGE, and retained full mitogenic activity on Balb/MK cells.
Receptor Bindinq Assays. Confluent Balb/MK
(IV-13) or NIH/3T3 (IV-14) cultures in 24-well plates were serum-starved for 24 h, then incubated with HEPES
binding buffer (100 mM HEPES, 150 mM NaCl, 5 mM KC1, 31.2 mM MgS04, 8.8 mM dextrose, and 0.1% BSA pH 7.4) containing l2sl-KGF for 3 h at 15°C. The cells were washed (3 x 1 ml) with cold PBS or, alternatively, washed with PBS (2 x 1 ml) followed by an extraction with PBS + 0.5 M NaCl (1 x 1 ml). The cells were lysed with 0.5% SDS (2 x 250 ~1), and radioactivity in the NaCl and SDS extracts of triplicate samples was measured in a ~ counter. l2sl-aFGF and -bFGF binding 6 ~ 5 2!~~~!:~e~~~
assays were performed similarly, except the salt extraction with 1.0 M NaCl (in aFGF binding assays) or 1.5 M NaCl (in bFGF binding assays) was substituted appropriately.
Bound counts/min were normalized according to protein content of SDS extracts as measured by BCA
protein assay (Pierce Chemical Co.). Specific binding was determined by subtracting normalized counts/min of samples incubated with 100-fold excess unlabelled ligand from the normalized counts/min bound in the absence of unlabelled ligand. In some experiments, heparin (3 ~g/ml) was added during the binding incubation. In competition assays, samples contained tracer levels of radiolabelled ligand (1 ng/ml) and several concentrations of competitor (0-300 ng/ml) for processing as outlined above. For Scatchard analysis, samples contained several concentrations of radiolabelled ligand (1-100 ng/ml) in the presence or absence of 100-fold excess unlabelled ligand and were processed similarly. Estimates of receptor affinity and total binding capacity were made using LIGAND
software (IV-15).
Cross-linking of 1251-KGF, -aFGFt and -bFGF to Receptors. Samples for covalent cross-linking were prepared from confluent serum-starved Balb/MK or NIH/3T3 cultures in 10-cm dishes using 10-30 ng/ml lasl-KGF, -aFGF, or -bFGF in the presence or absence of 100-fold excess unlabelled ligand. After binding (as described above), cross-linking with disuccinimidyl suberate was performed as described (IV-16). The cells were then scraped into cold HEPES binding buffer containing protease inhibitors (0.1 mM aprotinin and 1.0 mM phenylmethylsulfonyl fluoride), and a crude membrane fraction was generated by brief sonication (50 watts, lOs), low speed centrifugation (600 x g, 10 min), and high speed centrifugation (10,000 x g, 30 min) of the low speed supernatant. The membrane pellet was solubilized in sample buffer (IV-17) containing 100 mM dithiothreitol and boiled for 3 min. lzsl_Labelled proteins were resolved by 7.5% SDS-PAGE (IV-17) and autoradiography at -70°C.
Western Blot Detection of Phosphotyrosyl Proteins. Confluent cell cultures in 10-cm dishes were serum-starved for 24 h and then treated with KGF, aFGF, or bFGF (30-100 ng/ml) for 10 min at 37°C. The medium was aspirated, and the cells were solubilized in cold HEPES buffer containing 1% Triton X-100, and protease and phosphatase inhibitors (IV-7). The lysate was cleared by centrifugation (14,000 x g, 3 min), and phosphotyrosyl proteins were immunoprecipitated with 2~~~ 3~8 aP-Tyr absorbed to GammaBind G-agarose. Phosphotyrosyl proteins were specifically eluted using 50 mM phenyl phosphate, diluted in sample buffer,and resolved by 7.5% SDS-PAGE. Proteins were then transferred to nitrocellulose and detected with aP-Tyr and lzsl_protein A as described (IV-7).
DISCUSSION O~ RESULTS
Specifis Binding of lasl_KGF to Receptors on Balb,/MK. Saturable specific binding of 'zsI_KGF to intact Balb/MK cells could be detected in the presence of low concentrations of heparin (1-3 ug/ml) or after briefly extracting the cell surface with 0.5 M NaCl.
In the absence of heparin or salt extraction, specific binding to Balb/MK was obscured by an excess of low affinity binding. Heparin appeared to block the binding of 'zsI-KGF to the salt-extractable cell surface or extracellular matrix component, as salt extractable counts/min were dramatically lower in its presence. At these low heparin concentrations (1-3 ~cg/ml), KGF
retained a potent mitogenic effect of Balb/MK cells.
In Figure IV-1, specific binding of lzsl_KGF
(1 ng/ml) to Balb/MK cells is depicted, expressed as femtomoles bound per lOs cells, competed by increasing concentrations (nM) of unlabeled KGF (0),aFGF (O), or 2~~~~
.....o bFGF (e). Values shown are the mean of triplicate samples ~ standard deviation (SD). Where no error bars are shown, the error is less than the symbol size.
Similar results were obtained using either low concentrations of heparin (1-3 ~,g/ml) or brief salt extraction to block low affinity ligand binding in all competition studies shown. In panel B, specific 'zsI-KGF binding on NIH/3T3 cells is displayed, competed by unlabeled KGF, aFGF, or bFGF. In panel C, specific binding of lzsl-aFGF (1 ng/ml) to Balb/MK cells is shown, competed by unlabeled KGF, aFGF, or bGFG. In panel D, specific 'zsI-aFGF binding on NIH/3T3 cells is displayed, competed by unlabeled KGF, aFGF or bFGFG.
Using either heparin or salt extraction to block low affinity binding 'zsI-KGF binding was reduced 50% by 0.05 nM KGF (Fig. IV-lA).
High affinity lzsl-KGF binding to Balb/Mk was competed by aFGF with 4-fold lower efficiency than by KGF (50% displacement at 0.2 nM aFGF, Fig. IV-lA).
bFGF also competed for 'zsI-KGF binding but with 20-fold lower efficiency than KGF (50% displacement at 1 nM, Fig. IV-lA). In the presence of heparin (1-3 ~,g/ml) or after brief salt extraction, specific high affinity binding of 'zsI-KGF to NIH/3T3 cells was not detected (Fig. IV-18), consistent with its lack of mitogenic activity for this cell type (IV-1). However, without Fd ~~
added heparin or prior salt extraction, low affinity binding of IuI_KGF to NIH/3T3 was observed, lzsl-aFGF
also bound specifically and with high affinity to Balb/MK cells and was competed with similar efficiency by aFGF and KGF (50%) displacement at 0.2 and 0.5 nM, respectively; (Fig. IV-1C). In contrast, bFGF competed 20-fold less efficiently than the other two ligands for 'zsI-aFGF binding (50% displacement at 4 nM; Fig.
IV-1C). Finally, high affinity lzsl-aFGF binding to NIH/3T3 fibroblasts was competed with similar efficiency by aFGF or bFGF (50% displacement at 0.2 and 0.3 nM, respectively) but was not competed by KGF at any concentration tested (Fig. IV-1D). Thus the pattern of lzsI-KGF and -FGF high affinity binding and competition exhibited by Balb/MK cells was fundamentally different from that of NIH/3T3 fibroblasts.
These results indicate that:
1) KGF and aFGF competed for the same site on Balb/MK, 2) NIH/3T3 cells lack high affinity KGF
binding and, 3 ) bFGF competed poorly for lzsI-aFGF
binding on Balb/MK, yet efficiently for lzsl_aFGF binding on NIH/3T3 cells, which distinguishes at least two receptors to which a FGF can bind, one with KGF cross_reactivity and one with bFGF cross-reactivity.
Scatchard Analysis of lzsl_KGF and 'zsI-aFGF
Binding. Scatchard analysis of lzsl_KGF binding on Balb/MK yielded a curvilinear pattern, most simply interpreted as representing two receptor subpopulations of different affinities (Fig. IV-2A). Values (expressed as femtomoles bound per ~,g of cell protein) are the mean of triplicate samples ~ SD. In the inset of Fig. IV_2A. Saturation binding of lzsI_KGF ( D ) and ~zsl_aFGF (O) on Balb/MK cells is expressed as ligand bound (femtomoles) per ~cg of cell protein. Values are the mean triplicate samples ~ SD. B, Scatchard analysis of lzsl_KGF specif is binding on Balb/MK cells in the presence of (O) or absence (o) of added heparin.
The higher affinity component predicted fewer than 5,000 sites/cell with dissociation constant (KD) of 25 pM, while the lower affinity component predicted approximately 65,000 sites/cell with a KD of 400 pM.
The 50$ effective dose (ED) of the recombinant KGF used in these studies was approximately 50 pM for Balb/Mk cells of Experimental Section II, suggesting that both receptor subpopulations described here may mediate KGF
mitogenic activity. Scatchard analysis of 'zsI-KGF
binding performed in the absence of salt extraction or heparin reversed an additional low affinity component that was not saturable under the conditions tested (Fig. IV-2B). We attribute this low affinity binding to cell-associated heparin-like moieties similar to those demonstrated previously for bFGF (IV-19).
Scatchard analysis of l2sl_aFGF binding on Balb/MK revealed a single receptor population consisting of approximately 80,000 sites/cell with a KD
of 350 pM (Fig. IV-2A). Similar analysis of ~ZSI-aFGF
binding on NIH/3T3 fibroblasts revealed a single receptor population of approximately 100,000 sites/cell with a KD of 250 pM. These affinity and capacity values are within the range of values previously published for the high affinity aFGF receptor (KD = 50-500 pM, 0.5-5 x 104 sites/cell (IV-3)) and bFGF
receptor (KD = 10-200 pM, 0.2-10 x 104 sites/cell (IV-3, 19)). This work also confirmed the added presence of low affinity receptors for the FGFs on both Balb/MK
and NIH/3T3, similar to the low affinity bFGF receptors on BHK cells previously characterized by Moscatelli (IV-19).
Cross-linkinq of l~sI-KGF. -aFGF, and -bFGF to Their Receptors. Covalent affinity cross-linking of I~sI-KGF to its receptor on Balb/MK cells revealed two labelled species of 162 and 137 kDa that were specifically competed by 100-fold excess KGF (Fig. IV-~1 E ~ ~ Cl ~~,~s~,~~
3). Assuming a 1:1 receptor:ligand interaction, the corrected molecular masses of the putative KGF
receptors were 140 and 115 kDa, respectively.
Predictably, no proteins were specifically labelled when 'zsI-KGF was cross-linked to NIH/3T3 fibroblasts (Fig. IV-3). Similar results were obtained when heparin was added or after brief salt extraction suggesting that only high affinity KGF receptors were cross-linked by this method. On Balb/MK cells, Izsl_ aFGF was specifically cross-linked to two proteins, one similar in size to the larger species labelled by 'zsI_ KGF and one with a corrected molecular mass of 120 kDa (Fig. IV_3). On NIH/3T3 cells, however, 'zsI-aFGF bound specifically to two species with corrected molecular masses of 155 and 135 kDa (Fig. IV-3). Attempts to cross-link 'zsI_bFGF to receptors on Balb/MK indicated weak cross-reactivity with the KGF/aFGF-associated species, but on NIH/3T3 fibroblasts, 'zsI-bFGF and lzsl-aFGF appeared to label similar protein species (Fig.
IV-3).
Both protein species cross-linked to 'zsI-KGF
on Balb/Mk cells were efficiently competed with excess aFGF, and conversely, excess KGF competed for the 'zsI_ aFGF-labelled species. These results are consistent with the binding studies described above and, together with the size similarity of species labelled by KGF and aFGF indicates that both ligands may act through the same receptors on Balb/MK. Furthermore, the data indicate that an analogous situation may exist for FGFs acting on NIH/3T3 fibroblasts. bFGF competed efficiently for both l~sI-aFGF-labelled species, and conversely, aFGF competed for both luI-bFGF-labelled species; KGF did not compete for any cross-linked species on NIH/3T3 cells. Together with the size and similarity of proteins labelled by 'ZSI-aFGF and '2sI-bFGF
on fibrobhasts, the results support previous reports that aFGF and bFGF cross-react with two receptors of approximately 150 and 130 kDa (IV-18).
Phosphotyrosyl Proteins Induced by KGF, aFGF, and bFGF. To enrich for phosphotyrosyl proteins, cell lysates were sequentially immunoprecipitated and immunoblotted with affinity-purified antiphosphotyrosine antibodies. By this method several phosphotyrosyl proteins were observed in quiescent Balb/MK and NIH/3T3 cells (Fig. IV-4). KGF (30 ng/ml) specifically stimulated the rapid tyrosine phosphorylation of a 90-kDa protein (pp90) in Balb/MK
cells (Fig. IV-4). KGF-induced phosphorylation of pp90 in Balb/Mk cells reached maximum within 10 min at 37°C
and decreased thereafter. The phosphorylation of pp90 was dose-dependent and was detectable using KGF
concentrations from 10 to 100 ng/ml. aFGF also induced 2~~~~~8 the tyrosine phosphorylation of a 90-kDa protein in Balb/MK cells, although less effectively than KGF
(Fig. IV-4). pp90 was not observed in bFGF-treated Balb/MK cells over a concentration range of 10-100 ng/ml (Fig. IV-4). pp90 migrated similarly under reducing and non-reducing SDS-PAGE conditions, suggesting that it was not disulfide-linked to either of the higher molecular weight KGF-binding entities observed by covalent affinity cross-linking.
l0 A decrease in the electrophoretic mobility of a diffuse 70-kDa phosphotyrosyl protein (pp70) was also reproducibly observed in KGF- or aFGF-treated Balb/MK
cells and in FGF-treated NIH/3T3 cells (Fig. IV-4).
Such a shift indicates changes in phosphorylation of pp70 triggered by these growth factors.
KGF failed to induce tyrosine phosphorylation of any cellular proteins in NIH/3T3 fibroblasts (Fig.
IV-4), consistent with its lack of mitogenic effect as established in Experimental Section I or high affinity binding. In contrast, both aFGF and bFGF stimulated the phosphorylation of a 90-kDa protein in NIH/3T3 fibroblasts (Fig. IV-4), as reported previously in (IV-6). The FGFs also stimulated the phosphorylation of a 150-kDa protein in NIH/3T3 cells (Fig. IV-4) similar to that demonstrated by Friesel et al. (IV-7), and later reported by Ruta et al (IV-10) to be phospholipase Cy. The phosphorylation of phospholipase Cy on tyrosine by the epidermal growth factor and platelet-derived growth factor receptors was described previously (IV-21, 22).
Although both Balb/MK cells and NIH/3T3 fibroblasts displayed low affinity heparin-like receptors for KGF, the binding and cross-linking data of this Section IV show that only the Balb/MK
keratinocytes express high affinity KGF receptors.
Such high affinity receptors are believed to be required for transduction of the KGF mitogenic signal.
REFERENCEB FOR EBPERIMENTAL Ip IV-1. Rubin, J. S., Osada, H., Finch, P.W., Taylor, W. G., Rudikoff, S. and Aaronson, S. A. (1989) Proc. Natl. Acad.
Sci. U.S.A. 86, 802-806.
IV-2. Finch, P. W., Rubin, J. S., Miki, T., Ron, D, and Aaronson, S. A. (1989) Science, 245, 752-755.
IV-3. Burgess, W. H. and Maciag, T. (1989) Annu.
Rev. Biochem. 58, 575-606.
IV-4. Marics, I., Adelaide, J., Raybaud, F., Mattei, M-G., Coulier, J. P., 2~ z° ~~8 Lapeyriere, O. and Birnbaum, D. (1989) Oncogene 4, 335-340.
IV-5. Huang, S. S. and Huang, J. S. (1986) J.
Biol. Chem. 261, 9568-9571.
IV-6. Coughlin, S. R., Barr, P. J., Cousens, L. S., Fretto, L. J. and Williams, L. T.
(1988) J. Biol. Chem. 263, 988-993.
IV-7. Freisiel, R., Burgess, W. H. and Maciag, T. (1989) Mol. Cell. Biol. 9, 1857-1865.
IV-8. Imamura, T., Tokit, Y, and Mitsui, Y.
(1988) Biochem. Biophys. Res. Commum.
155, 583-590.
IV-9. Lee, P. L., Johnson, D.E., Cousens, L.S., Fried, V. A. and Williams, L. T.
(1989) Science 245, 57-60.
IV-10. Ruta, M., Burgess, W., Givol, D., Epstein, J., Neiger, N., Kaplow, J., Crumley, G., Dionne, C., Jaye, M. and Schlessinger, J. (1989) Proc. Natl.
Acad. Sci. U.S.A. 86, 8722-8726.
IV-11. White, M. F. and Kahn, C. R.
(1988) in Insulin Receptors, Part A: Methods for the Study of Structure and Function (Kahn, C. R., and Harrison, L., eds) pp. 125-145, Alan R. Liss, Inc., New York.
125-145.

2~~T~~~ ~~8 IV-12. Hunter, W. M. and Greenwood, F.
C.

(1962) Nature 194, 495-496.

IV-13. Weissman, B. E. and Aaronson, S. A.

(1983) Cel1 32, 599-606.

IV-14. Jainchill, J. L., Aaronson, S. A. and Todaro, G. J. (1969) J. Virol. 4, 549-553.

IV-15. Munson, P. J. and Rodbard, D. (1980) Anal. Biochem. 107, 220-239.

IV-16. Olwin, B. B. and Hauschka, S. D. (1986) Biochemistry 25, 3487-3492.

IV-17. Laemmli, U. K. (1970) Nature 2 27, 680-685.

IV-18. Neufeld, G. and Gospodarowicz, D. (1985) J. Biol. Chem. 260, 13860-1386 8.

IV-19. Moscatelli, D. (1987) J. Cell. Physiol.

131, 123-130.

IV-20. Burrus, L. W. and Olwin, B. B. (1989) J. Biol. Chem. 264, 18647-18653.

IV-21. Wahl, M. I., Nishibe, S., Suh, P-G., Rhee, S. G. and Carpenter, G. (1989) Proc. Natl. Acad. Sci. U.S.A. 86, 1568-1572.

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__ IV-23. Lobb, R. R., Harper, J. W. and Fett, J. W. (1986) Anal. Eiochem. 154, 114.
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IV-25. Ruta, M., Howk, R., Ricca, G., Drohan, W., Zabelshamsky, M., Laurevs, G., Barton, D. E., Frande, U., Schelessinger, J. and Givol, D. (1988) Oncogene 3, 9-15.

EXPERIMENTAL SECTION V
As established in Section IV, KGF exhibits specific binding to keratinocytes but not fibroblasts.
A cDNA library (4.5 x 106 independent clones) was prepared from BALB/MK epidermal keratinocytes (V-10) in an improved vector, ~pCEV27 (V-11), and transfected into NIH/3T3 mouse embryo fibroblasts (V-12) which synthesize KGF (V-13). Fifteen transformed foci were detected among a total of 100 individual cultures.
Each was shown to be resistant to 6418, indicating that it contained integrated vector sequences. Three representative transformants were chosen for more detailed characterization based on differences in their morphologies. Several plasmids were isolated from each transformant after plasmid rescue. This was accomplished by cloning genomic DNA from each transformant by one of the infrequent cutters that can release the plasmids containing cDNA inserts. Digested DNA was ligated under diluted conditions and used to transform bacterial-competent cells. Plasmids were isolated from ampicillin- and kanamycin-resistant transformants and used to transfect NIH/3T3 cells to examine for focus formation. The ecti plasmid was rescued by Xho I, while the ect2 and ect3 plasmids were rescued by Not I digestion. A single cDNA clone rescued from each transformant was found to possess ,.
high-titered transforming activity ranging from 103 to 104 focus-forming units per nanomole of DNA.
Transfectants induced by the individual plasmids containing these epithelial cell-forming cDNAs (designated ecti, ect2, and ect3) were used in subsequent analyses.
To determine if any of the three genes encodes for the KGF receptor, binding studies with recombinant 'uI-labeled KGF were performed. BALB/MK
cells showed specific high affinity binding of lzsl_ labeled KGF, which was not observed when NIH/3T3 cells were used. Expression of the ecti gene by NIH/3T3 cells resulted in the acquisition of 3.5-fold more 'uI-labeled KGF binding sites than BALB/MK cells. Under the same conditions, control NIH/3T3 as well as transfectants containing either ect2 or ect3 did not bind the labeled growth factor. These results determined that etcl encoded the KGF receptor (KGFR), whose introduction into NIH/3T3 cells had completed an autocrine transforming loop.
To characterize ecti, 4.2-kb cDNA released by Sal I digestion was transformed as a molecular probe to hybridize Sal I-digested genomic DNAs. Since Sal I is an infrequent cutter, the large genomic DNA fragments migrated near the origin of the gel. The expected 4.2-kb DNA fragment was detected in the ectl transformant 2~F~~~~~
(Fig. V-lA), but neither NIH/3T3 nor the other transfectants showed evidence of Sal I fragment hybridized by the cDNA insert. These results indicate that the ect2 and ect3 represented independent transforming genes. When Eco RI was used to cleave normal mouse DNA, several distinct ecti-hybridizing DNA
fragments were observed, which reflected endogenous ecti sequences or closely related genes (Fig. V-1B).
These ecti-related sequences were also observed in the DNAs of other species analyzed, including human, indicating its high degree of conservation in vertebrate evolution. A single ecti transcript of around 4.2 kb was observed in BALB/MK cells (Figure V-iC). Thus, the cDNA clone represented essentially the complete ectl transcript. In NIH/3T3 cells, a transcript of comparable size was only faintly detectable under stringent hybridization conditions.
Thus, if this transcript were to represent ectl rather than a related gene, its expression was markedly lower in fibroblasts as compared to epithelial cells.
Method for Genomic Analysis of ectl DNA and Comparative RNA Expression.
Figure V-lA is a Southern analysis of the Sal I-digested DNAs from NIH/3T3 and its transformants.
The blot was probed with the entire ectl cDNA insert.

a 2~~~~ ~~~
Lane 1, NIH/ectl; lane 2, NIH/ect2; lane 3, NIH/ect3;
lane 4 NIH/3T3.
Figure V-1B is a Southern analysis of Eco RI-digested DNAs of different animal species (Clontech Labs, Inc.). The blot was probed with the 5'-half of the ectl cDNA insert (Fig. V-2B) and washed under reduced stringency conditions. Lane 1, human; lane 2, rhesus monkey; lane 3, mink; lane 4, cat; lane 5, mouse; lane 6, cow; lane 7, chicken; lane 8, dog, lane 9, guinea pig; lane 10, pig.
Figure V-1C is a Northern analysis of NIH/3T3 and BALB/MK RNA. The blot was probed with the 5'-half of the ecti cDNA (lanes 1 and 2) or a /3-actin cDNA
(lanes 3 and 4) and washed under stringent conditions.
Lanes 1 and 3, NIH/3T3; lanes 2 and 4, BALB/MK.
In the above analysis of V-lA and V-1B, the blot was probed by digesting DNA (10 ~,g) by Sal I (Fig.
V-lA) or Eco RI (Fig. V-1B), fractionated by agarose gel electrophoresis, and transferred to a nylon-supported nitrocellulose paper (Nitrocellulose-GTG, FMC
Corp.). The blot in Fig. V-lA was hybridized with the 32p-labeled entire ectl insert at 42°C and washed at 65°C in 0.1 x saline sodium citrate (SSC), while the blot in Fig. V-iB was hybridized with the 32P-labeled 5'-ectl probe (Fig. V-2B) at 37°C and washed at 55°C in 0.1 x SSC. Hybridization experiments were performed at the indicated temperature in a solution containing 50%
formamide, 5 x SSC, 2.5 x Denhardt solution, 7 mM tri-HC1 (pH 7.5), denatured calf thymus DNA (0.1 mg/ml), and tRNA (0.1 mg/ml). Location of DNA markers (BRL, Gaithersburg, MD) is indicated in kilobases.
Polyadenylated RNA preparations (5 ,ug each) were fractionated by formaldehyde gel, transferred to Nitrocellulose-GTG, and hybridized with the 5~-ecti probe for the Northern analysis of V-iC. After autoradiography, the filter was boiled to remove the probe and then hybridized with a /3-actin probe (lanes 3 and 4). Location of markers (BRL, Gaithersburg, MD) is indicated in kilobases.
Receptor Nucleotide Sequence. Nucleotide sequence analysis of the 4.2-kb ectl cDNA revealed a long open reading frame of 2235 nucleotides (nucleotide position 562 to 2796). Two methionine codons were found at nucleotide positions 619 and 676 respectively.
The second methionine codon matched the Kozak~s consensus for a translational initiator sequence (A/GC-CATGG) (V-15). Moreover, it was followed by a characteristic signal sequence of 21 residues, 10 of which were identical to those of the putative signal peptide of the mouse basic FGF (bFGF) receptor (V-16,17). Thus, the second AGT is the initiation codon.
The receptor polypeptide is believed therefore to ~~~!~> ~~~
comprise 707 amino acids with a predicted size of 82.5-kD (Fig. V-2A).
The amino acid sequence predicted a transmembrane tyrosine kinase closely related to the mouse bFGF receptor (bFGFR). The percent similarity between both proteins is shown in Fig. V-2B. The putative KGFR extracellular portion contained two immunoglobulin (Ig)-like domains, exhibiting 77% and 60% similarity with the Ig-like domains 2 and 3, respectively, of the mouse bFGFR. The sequence NHZ-terminal to the first Ig-like domain of the KGFR is 63 residues long in comparison to 88 residues found in the shorter form of the mouse bFGFR. The mouse bFGFRs contain a stretch of eight consecutive acidic residues between the first and second Ig-like domains (V-16-18).
The KGFR lacked such an acidic amino acid domain (Fig.
V-2B).
The kinase domain of the KGFR was 90% related to the bFGFR tyrosine kinase (Fig. V-2B). The central core of the catalytic domain was flanked by a relatively long juxtamembrane sequence, and the tyrosine kinase domain was split by a short insert of 14 residues, similar to that observed in mouse, chicken, and human bFGF receptors (V-16-19). Hanafusa and co-workers isolated a partial cDNA for a tyrosine kinase gene, designated bek, by bacterial expression cloning with phosphotyrosine antibodies (V-20). The reported sequence of bek was identical to the KGFR in the tyrosine kinase domain (Fig. V-2B).

'~
f"r '...J e~ tJ
Method for Determining Prima~,v Structure of the KGF Receptor.
Figure V-2A is the amino acid sequence deduced for the coding region of the KGF receptor cDNA.
Amino acids are numbered from the putative initiation site of translation. Potential sites of N-linked glycosylation are underlined. The potential signal peptide and transmembrane domains are boxed. The interkinase domain is shown by underlined italic letters. Glycine residues considered to be involved in ATP (adenosine triphosphate) binding are indicated by asterisks. Cysteine residues delimit two Ig-like domains in the extracellular portion of the molecule are shown by bold face. Nucleotide sequence was determined by the chain termination method (V-30).
Figure V-28 is a structural comparison of the predicted KGF an bFGF receptors. The region used as a probe for Southern and Northern analysis (Fig. V-iB and C) is indicated. The region homologous to the published bek sequence (V-20) is also shown. The schematic structure of the KGF receptor is shown below the restriction map of the cDNA clone. Amino acid sequence similarities with the smaller and larger bFGF receptor variants are indicated. S, signal peptide; A, acidic region; IG1, IG2 and IG3, Ig-like domains; TM, transmembrane domain, JM, juxtamembrane domain; TK1 and TK2, tyrosine kinase ~;~ ~ !. > v , i EJ a~!
domains; IK, interkinase domain; C, COOH-terminus domain.
Competitive Bindincr Scatchard analysis of 1~I-labeled KGF binding to the NIH/ectl transfectant revealed expression of two similar high-affinity receptor populations. Out of a total of ~3.8 x 105 sites per cell, 40% displayed a dissociation constant (Kd) of 180 pM and the remaining 60% showed a kd of 480 pM as demonstrated in Section IV. These values are comparable to the high-affinity KGF binding sites displayed by BALB/MK cells (V-9). The pattern of KGF
and FGF competition for lzsl_labeled KGF binding to NIH/ecti cells was also very similar to that observed with BALB/MK cells (Fig. V-3). Although maximum '~I-labeled KGF binding to NIH/ecti cells was 3.5 times higher than to BALB/MK, there was 50% displacement by 2 ng/ml of either KGF or acidic FGF (aFGF) with each cell type. Similarly, both cells showed 15 times less efficient competition by bFGF for bound 1~I-labeled KGF. Thus, the cloned KGFR exhibited the characteristic pattern of KGF and FGF competition displayed by BALB/MK cells, which indicates that the KGFR is a high-affinity receptor for aFGF as well as KGF.
When 1~I-labeled KGF is cross-linked to its receptors on BALB/MK cells, two protein species of 162-:J !J ..... t.~ x~' 2 ~~ '=~ 'A '~~ ~' e'~
and 137-kD have been observed as established in Section IV. Taking into account the size of KGF itself, we have estimated the cross-linked receptors to be around 140- and 115-kD, respectively. When lzsl-labeled KGF
cross-linking was performed with NIH/ectl cells, a single species corresponding in size to the smaller, 137-kD complex in BALB/MK cells (Fig. V-4A) is observed. Moreover, detection of this band was specifically and efficiently blocked by unlabeled KGF.
'When glycosylation is considered, the size of the KGFR
predicted by sequence analysis corresponds reasonably well with the corrected size (115 kD) of the cross-linked KGFR in the ecti transfectant.
To examine the functional nature of the KGFR
expressed in NIH/ectl cells, its capacity to induce tyrosine phosphorylation of cellular proteins was investigated. NIH/3T3 or NIH/ecti cells were exposed to KGF for 10 min and cell lysates were subjected to immunoprecipitation and immunoblotting analysis with antibody to phosphotyrosine (anti-Ptyr). NIH/ecti cells contained several tyrosine-phosphorylated proteins that were not detectable in control or KGF-stimulated NIH/3T3 cells (Fig. V-4B). Addition of KGF
to NIH/ecti cells resulted in the detection or increased tyrosine phosphorylation of several putative substrates. These included p55, p65, p90, p115, p150 ~.
and p190. These findings established that the KGFR was enzymatically activated in response to KGF. In Section IV, it was noted that similar-size proteins are phosphorylated in response to KGF triggering of BALB/MK
cells. Moreover, the 115-kD phosphoprotein matched the corrected size of the KGFR cross-linked by l2sl_labeled KGF.
Method for Analysis of the KGF Receptor Expressed in NIH~/3T3 Cells.
Figure V-4A shows covalent affinity cross-linking of l2sl-labeled KGF to BALB/MK, NIH/3T3, and NIH/ectl cultures. The left and center panels of this autoradiogram were exposed to Kodak XAR film for 72 hours at -70°C; the right lane is an 18-hour exposure of the same autoradiogram. The second lane (labeled +) for each cell type shows cross-linking performed in the presence of excess unlabeled KGF. Molecular weight markers (x 10'3) are indicated on the left; the positions of 'ZSI-labelled KGF-cross-linked complexes are indicated by arrows. Cross-linking was carried out as described previously in Section IV.
Figure V-4B shows autoradiogram of phosphotyrosyl-proteins from intact NIH/3T3 and NIH/ectl cells before and after treatment with KGF.
Molecular weight markers are indicated on the left; the estimated molecular weights of proteins displaying KGF-* trade mark '' , 2~~~°~~~
stimulated phosphorylation on tyrosine are shown at right. Analysis of phosphoproteins was performed as described previously in Section IV.
REFERENCES FOR EXPERIMENTAL V
V-1. Guroff, G. et al, Oncogenes, Genes and Growth Factors, G. Block and J. Marsh, Eds. (Wiley-Interscience, New York, 1987) pp. 191-224;
Kahn, P. and Graf, T., Eds. Oncogenes, Genes and Growth Control (Springer Verlag, New York, 1986).
V-2. Okayama, H. and Berg, P., Mol. Cell. eiol.
3:280 (1983); Aruffo, A. and Seed, B., Proc.
Natl. Acad. Sci. U.S.A. 84:8573 (1987).
V-3. Pierce, J.H. et al, Science 239:628 (1988).
V-4. Gazit, A. et al, Cell, 39:89 (1984); Julius, D. et al, Science 244:1057 (1989).
V-5. Miki, T. et al, Gene 83:137 (1989).
V-6. Matsui, T. et al, Science 243:800 (1989);
Kraus, M.H. et al, Proc. Natl. Acad. Sci.
U.S.A. 86:9193 (1989); Kruh, G.D. et al, ibid 87:5802 (1990).

~~e~ ~ciR~~
V-7. Rubin, J.S. et al, Proc. Natl. Acad. Sci.
U.S.A. 86:802 (1989).
V-8. Finch, P.W. et al, Science 245:752 (1989).
V-9. Bottaro, D.P. et al. J. Biol. Chem. 265:12767 (1990) .
V-10. Weissman, B.E. and Aaronson, S.A., Cell 32:599 (1983).
V-11. Miki, T. unpublished data.
V-12. Jainchill, J.L.; Aaronson, S.A.; Todaro, G.J., J. Virol. 4:549 (1969).
V-13. Rubin, J.S., unpublished data.
V-15. Kozak, M. Nucleic Acids Res. 5:8125 (1987).
V-16. Safran, A. et al, Oncogene 5:635 (1990).
V-17. Reid, H.H., Wilks, A.F., Bernard, O., Proc.
Natl. Acad. Sci. U.S.A. 87:1596 (1990;
Mansukuhani, A; Moscatelli D; Talarico, D;
Levytska, V; Basilico, C; ibid p.4378.

V-18 Lee, P.L.; Johnson, D.E.; Cousens; L.S., Fried; V.A; Williams, L.T., Science 245:57 (1989); Pasquale, E.B. and Singer, S.J. Proc.
Natl. Acad. Sci. U.S.A. 86:8722 (1989).
V-19. Ruta, M. et al Oncogene 3:9 (1988); Ruta, M.
et al, Proc. Natl. Acad. Sci. U.S.A. 86:5449 (1989).
V-20. Kornbluth S, Paulson, K.E., Hanafusa, H.;Mol.
Cell. Bio1.8:5541 (1988).
V-22. Betsholtz, C; Johnsson, A; Heldin C.-H.;
Westermark, B., Proc. Natl. Acad. Sci. U.S.A
83:6440 (1986); Fleming, T.P. et al, ibid 86:8063 (1989).
V-24 Dionne, C.A. et al, EMBO J. 9:2685 (1990) V-25 Pasquale, E.B., Proc, natl. Acad. Sci. U.S.A.
87:5812 (1990).
V-26. Hattori, Y. et al, ibid p. 5983.
V-27. Johnson, D.E.; Lee, P.L.; Lu, J.; Williams, L.T., Mol. Cell. Biol. 10:4728 (1990).

~a~.~~
V-30 Sanger, F.; Nicklen, S.; Coulson, A.R., Proc.
Natl. Acad. Sci. U.S.A. 74:5463 (1977).

2~~~ 3 ~~
.~
For the purposes of completion, the background description and present disclosure, each of the published articles, patents and patent applications heretofore identified in this specification are hereby incorporated by reference into the specification.
The foregoing invention has been described in some detail for purposes of clarity and understanding.
It will also be obvious that various combinations in form and detail can be made without departing from the scope of the invention.

- -at Table I-1. Growth Factor Purification Purification Protein Total Spheifie atp ~ctivit~r ~etivitr ~

(gyp) (units) (unit~/p) Conditiornd oadiu~ t.i x 103 2.S x t0~ t.a x 10t ~t0 ltt~n) Ultr~filtration 1.3 x t03~ 3.t x 10; t.S x tot (ret~ntat~) NSAC 0.734 t.b x t0~ t.t x ' t0~

0.6 111 ilaCl pool _ tSIC-03000 S1r a.4 x t0'~ L7 x t03 3.t x 10s c~NPVC s.t x to'~' Z.t x tot 3.s x toy Recoveries were calculated by assuming that all of the mitogenic activity in the starting material was due to the isolated factor.
' One unit of activity is def ined as half of the maximal stimulation of thymidine incorporation induced by TSK-purified factor in the Balb/MK bioassay, in which approximately 3 ng of the TSK-purified factor stimulated 1 unit of activity.
' Protein was estimated by using the Bradford reagent from BioRad (I-23).
1~
°Protein was estimated by using Azia = 140.

F~ ~
~~

".,...
' 'Hi Table I-2. Target Cell Speciricity of Growth Factors Growth faetx E~ithalial ftbrobtast Endothaitat IAlMC tS/Sa9 CCLZOd Ilu~n saphanous 11111/313=

vein ~caf soo-~ooot-a s-~o <s EGf 100I00 20-i0 10-3010-ZO n.d.

TGIa 1so-300 n.d, n.d. 10-t0 n.d.

afof 3oo-soo t-a s-~o so-ro s dfcf Sao-too t-3 :-s so-ro s to Comparison of maximal thymidine incorporation stimulated by KGF and other growth factors in a variety of cell lines, expressed as fold stimulation over background.
This data represents a summary of four different experiments.
'Maximal stimulation by aFGF required the presence of heparin (Sigma) , 20 ~Cg/ml.
n.d. - not determined.

~'~~~ ~ ~~S
TABLE II-1. Effect of Heparin on KGF Mitogenic Activity.
G~ovth Factor ~ wlNnT3 is0 9.s <1 <t ~faf 106 2S9 10.i bd oaf so ~_~ as.~ r~
Cells were plated in microtiter plates, grown to confluence in serum containing media and then placed in a serum-free medium for 24-72 hr prior to sample addition. Mitogenesis assays were performed as described (see Experimental Section I, above and II-3).
Where indicated, heparin was included in the culture media at a final concentration of 20 ~,g/ml. The concentration of all growth factors was 50 ng/ml. The results represent fold stimulation of 3H-thymidine incorporation in the indicated assay cell in the presence (+) or absence (-) of heparin. Each value represents the mean result from two independent experiments in which each point, in turn, represents the mean value of duplicate analyses.

Claims (20)

1. A chimeric protein which comprises within a single polypeptide molecule a segment of KGF protein, wherein the segment of KGF protein is a segment of amino acids of Figure 11-1B which comprises a sufficient number of consecutive amino acids 32 - 189 of Figure II-1B to confer on the polypeptide molecule preferential mitogenic activity for an epithelial cell, and at least one other polypeptide portion of the fibroblast growth factor family.

2. The chimeric protein of claim 1, wherein the fibroblast growth factor is acidic fibroblast growth factor (aFGF) .

3. The chimeric protein of claim 1, wherein the polypeptide portion of aFGF is defined by amino acid residues in that portion of aFGF that is homologous to the KGF sequence responsible for heparin sensitivity of KGF.

4. The chimeric protein according to claim 2, wherein said segment of KGF protein is defined by amino acid residues numbered 32 through 78 in Figure II-1B and said polypeptide portion of aFGF is defined by the carboxyl terminus of aFGF, beginning at residue number 39.

5. A pharmaceutical composition comprising a chimeric protein according to any one of claims 1 to 4, and a pharmaceutically acceptable carrier.

6. Use of a chimeric protein according to any one of claims 1 to 4 in a composition for the treatment of conditions requiring specific stimulation of epithelial cells in the presence of heparin.

7. Use of a chimeric protein according to any one of claims 1 to 4 for preparing a medicament for acceleration or improvement of wound healing involving the epidermis.

8. Use of a chimeric protein according to any one of claims 1 to 4 for preparing a medicament that is to be topically applied to the skin or eye.

9. An antibody specifically directed against glycosylated or unglycosylated keratinocyte growth factor (KGF) polypeptide selected from the group consisting of(a) the following amino acid sequence, (b) a portion of the following amino acid sequence without the N-terminal 31 amino acids, and (c) an amino acid sequence that differs by the addition, deletion, or substitution of one or more amino acids from the following amino acid sequence:
M H K W I L T W I L P T L L Y
R S C F H I I C L V G T I S L
A C N D M T P E Q M A T N V N
C S S P E R H T R S Y D Y M E
G G D I R V R R L F C R T Q W
Y L R I D K R G K V K G T Q E
M K N N Y N I M E I R T V A V
G I V A I K G V E S E F Y L A
M N K E G K L Y A K K E C N E
D C N F K E L I L E N H Y N T

Y A S A K W T H N G G E M F V

A L N Q K G I P V R G K K T K

K E Q K T A H F L P M A I T

10. The antibody of claim 9 which neutralizes the mitogenic activity of human KGF.

11. The antibody of claim 9 which is a polyclonal antibody.

12. The antibody of claim 9 which is a monoclonal antibody.

13. The antibody of claim 9 which reacts against the peptide NDMTPEQMATNVR.

14. A pharmaceutical composition comprising at least one antibody of claim 9 and a pharmaceutically acceptable carrier.

15. Use of the antibody of any one of claims 9 to 13 for preparing a medicament for treating conditions requiring specific inhibition of stimulation of epithelial cells by KGF.

16. A recombinant DNS molecule which encodes a chimeric protein according to claim 1.

17. A recombinant DNA molecule which encodes a chimeric protein according to claim 2.

18. A recombinant DNA molecule which encodes a chimeric protein according to claim 3.

19. A recombinant DNA molecule which encodes a chimeric protein according to claim 4.

20. A method for detecting a KGF protein in a sample comprising:
i) reacting polypeptides from body fluids or tissue samples with an antibody of claim 9 to form an antibody-polypeptide complex; and ii) detecting said complex.