CA2055966A1 - Cell culture insert - Google Patents

Cell culture insert

Info

Publication number
CA2055966A1
CA2055966A1 CA2055966A CA2055966A CA2055966A1 CA 2055966 A1 CA2055966 A1 CA 2055966A1 CA 2055966 A CA2055966 A CA 2055966A CA 2055966 A CA2055966 A CA 2055966A CA 2055966 A1 CA2055966 A1 CA 2055966A1
Authority
CA
Canada
Prior art keywords
insert
cell culture
break
away
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2055966A
Other languages
French (fr)
Other versions
CA2055966C (en
Inventor
Oresta Natalia Fedun
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
Original Assignee
Oresta Natalia Fedun
Becton, Dickinson And Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oresta Natalia Fedun, Becton, Dickinson And Company filed Critical Oresta Natalia Fedun
Publication of CA2055966A1 publication Critical patent/CA2055966A1/en
Application granted granted Critical
Publication of CA2055966C publication Critical patent/CA2055966C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02—Membranes; Filters
    • C12M25/04—Membranes; Filters in combination with well or multiwell plates, i.e. culture inserts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates

Abstract

A cell-supporting membrane separation device is provided in a cell culture insert to ease the use of conventional instrumentation for subsequent examination. The cell culture insert is provided with a microporous membrane (16) forming the bottom wall as the cell culturing surface of the insert. The insert includes a support mechanism (20) for holding the insert suspended in a selected position in a multi-well plate for culturing, and a break-away mechanism (22) to separate the support mechanism from the membrane supporting portion of the insert, once the degree of cell culture activity has taken place. The break-away concept of the invention accommodates several different embodiments of insert with an appropriate break-away pattern. <IMAGE>
CA002055966A 1990-12-19 1991-11-21 Cell culture insert Expired - Fee Related CA2055966C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US63011690A 1990-12-19 1990-12-19
US07/630,116 1990-12-19

Publications (2)

Publication Number Publication Date
CA2055966A1 true CA2055966A1 (en) 1992-06-20
CA2055966C CA2055966C (en) 1995-08-01

Family

ID=24525845

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002055966A Expired - Fee Related CA2055966C (en) 1990-12-19 1991-11-21 Cell culture insert

Country Status (9)

Country Link
US (1) US5578492A (en)
EP (1) EP0495213B1 (en)
JP (1) JPH0866B2 (en)
AT (1) ATE122388T1 (en)
AU (1) AU643852B2 (en)
CA (1) CA2055966C (en)
DE (1) DE69109651T2 (en)
DK (1) DK0495213T3 (en)
ES (1) ES2071899T3 (en)

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US20080076170A1 (en) * 2006-09-27 2008-03-27 Tuija Annala Cell culture insert and cell culture vessel
CA2671967A1 (en) * 2006-12-07 2008-06-19 Wilson Wolf Manufacturing Corporation Highly efficient devices and methods for culturing cells
US8163549B2 (en) 2006-12-20 2012-04-24 Zimmer Orthobiologics, Inc. Method of obtaining viable small tissue particles and use for tissue repair
US20090012629A1 (en) 2007-04-12 2009-01-08 Isto Technologies, Inc. Compositions and methods for tissue repair
DE102007043614B3 (en) 2007-09-13 2008-11-20 Biocrates Life Sciences Gmbh Retainer for wells of multi-corrugated plate, has support arranged in opening below retaining bar, where retainer rests with lateral outer surfaces of support bars, retaining bar and/or connecting rods against inner side of opening
EP2297295A4 (en) * 2008-07-08 2013-11-27 Wolf Wilson Mfg Corp Improved gas permeable cell culture device and method of use
GB0817990D0 (en) * 2008-10-02 2008-11-05 Reinnervate Ltd Cell culture vessel
FR2942239B1 (en) * 2009-02-18 2013-07-26 Oreal CELL OR TISSUE CULTURE DEVICE AND ASSOCIATED ASSEMBLY
FR2942240B1 (en) * 2009-02-18 2011-03-18 Oreal CELL OR TISSUE CULTURE DEVICE WITH ADAPTER AND ASSOCIATED CELL OR TISSUE CULTURE ASSEMBLY
DE202011003049U1 (en) * 2011-02-22 2011-04-28 Sabeu Kunststoffwerk Northeim Gmbh Use for cell cultures
EP2653531A1 (en) * 2012-04-18 2013-10-23 Oxyphen AG Culture assembly
US20140178343A1 (en) 2012-12-21 2014-06-26 Jian Q. Yao Supports and methods for promoting integration of cartilage tissue explants
CA2972094C (en) 2015-01-12 2023-10-24 Wake Forest University Health Sciences Multi-layer skin substitute products and methods of making and using the same
EP3368649B1 (en) * 2015-10-26 2022-08-03 Dramedica, LLC Culture insert assembly and system for culture, transfer, and analysis
US20190309245A1 (en) * 2015-12-26 2019-10-10 Nepa Gene Co., Ltd. Vessel and method for cell isolation, culture and proliferation
DE102017007169A1 (en) 2016-08-18 2018-02-22 Brand Gmbh + Co Kg Cell culture insert and apparatus for cultivating cells
CN109642200B (en) * 2016-08-18 2023-04-04 普兰德有限两合公司 Cell culture insert and device for culturing cells
KR20180091763A (en) 2017-02-06 2018-08-16 포항공과대학교 산학협력단 Cell culture insert and cell culture container, and method for fabricating the same
JP7441521B2 (en) * 2018-02-28 2024-03-01 株式会社幹細胞＆デバイス研究所 Cell holding and transport device
KR102068465B1 (en) 2018-04-18 2020-01-21 주식회사 포스코 Cell culture structure, manufacturing method thereof and Cell culture device including the cell culture structure
JP1626726S (en) * 2018-06-20 2019-03-18
JP1627100S (en) * 2018-06-20 2019-03-18
WO2020001733A1 (en) 2018-06-25 2020-01-02 Universitätsklnikum Hamburg-Eppendorf A holding device for microscopy applications and microscopy chamber
CN109055219B (en) * 2018-09-17 2023-07-14 重庆医科大学附属儿童医院 Cell wound healing experimental device
JP7265243B2 (en) * 2019-01-15 2023-04-26 ネッパジーン株式会社 cell culture insert
KR102167104B1 (en) * 2020-01-30 2020-10-16 주식회사 에스티원 Permeable membrane support with a detachable permeable membrane
US20210309952A1 (en) * 2020-04-06 2021-10-07 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Systems for cell plate co-culture
USD946167S1 (en) * 2020-10-16 2022-03-15 Emd Millipore Corporation Cell culture hanging insert
CN116783276A (en) * 2020-12-28 2023-09-19 分子装置(奥地利)有限公司 Microwell plate wells for cell culture
DE102021132560A1 (en) * 2021-12-09 2023-06-15 Sabeu Gmbh & Co. Kg Cell culture sampling device
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Also Published As

Publication number Publication date
ES2071899T3 (en) 1995-07-01
JPH053779A (en) 1993-01-14
EP0495213A1 (en) 1992-07-22
ATE122388T1 (en) 1995-05-15
CA2055966C (en) 1995-08-01
DE69109651D1 (en) 1995-06-14
AU8818391A (en) 1992-06-25
AU643852B2 (en) 1993-11-25
JPH0866B2 (en) 1996-01-10
US5578492A (en) 1996-11-26
EP0495213B1 (en) 1995-05-10
DE69109651T2 (en) 1995-09-07
DK0495213T3 (en) 1995-09-11

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