CA2061382A1 - Method for producing cysteine-free peptide - Google Patents

Method for producing cysteine-free peptide

Info

Publication number
CA2061382A1
CA2061382A1 CA002061382A CA2061382A CA2061382A1 CA 2061382 A1 CA2061382 A1 CA 2061382A1 CA 002061382 A CA002061382 A CA 002061382A CA 2061382 A CA2061382 A CA 2061382A CA 2061382 A1 CA2061382 A1 CA 2061382A1
Authority
CA
Canada
Prior art keywords
free peptide
cysteine
producing cysteine
producing
peptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002061382A
Other languages
French (fr)
Other versions
CA2061382C (en
Inventor
Osamu Nishimura
Masato Kuriyama
Noboyuki Koyama
Tsunehiko Fukuda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Takeda Pharmaceutical Co Ltd
Original Assignee
Osamu Nishimura
Masato Kuriyama
Noboyuki Koyama
Tsunehiko Fukuda
Takeda Chemical Industries, Ltd.
Takeda Pharmaceutical Company Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27284805&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2061382(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Osamu Nishimura, Masato Kuriyama, Noboyuki Koyama, Tsunehiko Fukuda, Takeda Chemical Industries, Ltd., Takeda Pharmaceutical Company Limited filed Critical Osamu Nishimura
Publication of CA2061382A1 publication Critical patent/CA2061382A1/en
Application granted granted Critical
Publication of CA2061382C publication Critical patent/CA2061382C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/08—Tripeptides
    • C07K5/0821—Tripeptides with the first amino acid being heterocyclic, e.g. His, Pro, Trp
    • C07K5/0823—Tripeptides with the first amino acid being heterocyclic, e.g. His, Pro, Trp and Pro-amino acid; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/50—Fibroblast growth factors [FGF]
    • C07K14/503—Fibroblast growth factors [FGF] basic FGF [bFGF]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/605—Glucagons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/635—Parathyroid hormone (parathormone); Parathyroid hormone-related peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/41—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a Myc-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/735—Fusion polypeptide containing domain for protein-protein interaction containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Abstract

A cysteine-free peptide is produced by producing a fused protein comprising a protein having cysteine at its N-terminal and a cysteine-free peptide ligated to the N-terminal and subsequently subjecting the fused protein to a reaction for cleaving the peptide linkage.
CA002061382A 1991-02-19 1992-02-18 Method for producing cysteine-free peptide Expired - Fee Related CA2061382C (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
JP024841-1991 1991-02-19
JP2484191 1991-02-19
JP27143891 1991-10-18
JP0271438-1991 1991-10-18
JP0277724-1991 1991-10-24
JP27772491 1991-10-24

Publications (2)

Publication Number Publication Date
CA2061382A1 true CA2061382A1 (en) 1992-08-20
CA2061382C CA2061382C (en) 2003-02-18

Family

ID=27284805

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002061382A Expired - Fee Related CA2061382C (en) 1991-02-19 1992-02-18 Method for producing cysteine-free peptide

Country Status (7)

Country Link
US (2) US5861284A (en)
EP (1) EP0499990B1 (en)
KR (1) KR100249534B1 (en)
AT (1) ATE138102T1 (en)
CA (1) CA2061382C (en)
DE (1) DE69210645T2 (en)
ES (1) ES2087323T3 (en)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0499990B1 (en) 1991-02-19 1996-05-15 Takeda Chemical Industries, Ltd. Method for producing cysteine-free peptides
EP0619119B1 (en) * 1991-12-09 1999-03-31 Asahi Kasei Kogyo Kabushiki Kaisha Stabilized parathyroid hormone composition
CZ315594A3 (en) * 1992-06-15 1995-07-12 Pfizer Peptide of glucagon type, insulinotropic derivatives, process of their preparation, pharmaceutical composition containing such compounds and use
AU672790B2 (en) * 1992-07-15 1996-10-17 Novartis Ag Variants of parathyroid hormone and its fragments
WO1996017941A2 (en) * 1994-12-07 1996-06-13 Bionebraska, Inc. Production of c-terminal amidated peptides from recombinant protein constructs
AU2263197A (en) * 1996-02-06 1997-08-28 Eli Lilly And Company Diabetes therapy
EP0887417A3 (en) * 1997-06-27 1999-01-13 Takeda Chemical Industries, Ltd. Method of producing a 19P2 ligand by cleavage of a fusion protein containing it
KR100230578B1 (en) * 1997-07-25 1999-12-01 허영섭 Recombinant pth expression vector using chimeric phosphoribulokinase gene
WO2000036122A1 (en) * 1998-12-11 2000-06-22 Takeda Chemical Industries, Ltd. Process for producing apelin
CA2394404A1 (en) * 1999-12-17 2001-06-21 Takeda Chemical Industries, Ltd. Process for producing kiss-1 peptide
US6641820B1 (en) 2000-01-19 2003-11-04 Allergan, Inc. Clostridial toxin derivatives and methods to treat pain
US7138127B1 (en) 2000-01-19 2006-11-21 Allergan, Inc. Clostridial toxin derivatives and methods for treating pain
US6500436B2 (en) 2000-01-19 2002-12-31 Allergan, Inc. Clostridial toxin derivatives and methods for treating pain
KR100407792B1 (en) * 2000-08-02 2003-12-01 한국생명공학연구원 Preparation method of recombinant protein by use of human glucagon-derived peptide analogue as a fusion expression partner
US20040029215A1 (en) * 2000-12-07 2004-02-12 Masato Suenaga Process for producing rfrp
WO2003060125A1 (en) * 2002-01-11 2003-07-24 Takeda Chemical Industries, Ltd. PROCESS FOR PRODUCING KiSS-1 PEPTIDE
WO2003097690A2 (en) * 2002-05-16 2003-11-27 Theratechnologies Inc. Pth derivatives resistant to skin proteases
CA2497794A1 (en) * 2002-09-06 2004-03-18 Bayer Pharmaceuticals Corporation Modified glp-1 receptor agonists and their pharmacological methods of use
EP1539960B1 (en) * 2002-09-09 2010-04-28 Hanall Pharmaceutical Co., Ltd. Protease-resistant modified interferon alpha polypeptides
WO2004039392A2 (en) * 2002-10-29 2004-05-13 Alza Corporation Stabilized, solid-state polypeptide particles
ME01366B (en) 2003-11-21 2013-12-20 Nps Allelix Corp Production of glucagon like peptide 2 and analogs
US7998930B2 (en) 2004-11-04 2011-08-16 Hanall Biopharma Co., Ltd. Modified growth hormones
RS51319B (en) * 2005-09-22 2010-12-31 Biocompatibles Uk Limited Glp-1(glucagon-like peptide-1) fusion polypeptides with increased peptidase resistance
DE602006009631D1 (en) * 2006-05-10 2009-11-19 Biocompatibles Uk Ltd Spherical microcapsules containing GLP-1 peptides, their production and their use
JP5004165B2 (en) * 2006-10-10 2012-08-22 独立行政法人産業技術総合研究所 Protein suitable for protein orientation control immobilization
EP1972349A1 (en) * 2007-03-21 2008-09-24 Biocompatibles UK Limited GLP-1 fusion peptides conjugated to polymer(s), their production and use
EP1975176A1 (en) * 2007-03-27 2008-10-01 Biocompatibles UK Limited Novel glp-1 fusion peptides, their production and use
BRPI0819212A2 (en) * 2007-10-23 2015-06-16 Allergan Inc Methods of Treatment of Chronic Neurogenic Inflammation Using Modified Clostridium Toxins
EP2163243A1 (en) * 2008-09-12 2010-03-17 Biocompatibles UK Limited Treatment of acute myocardial infarction (AMI) using encapsulated cells encoding and secreting GLP-1 peptides or analogs thereof
JP5738027B2 (en) 2010-03-30 2015-06-17 キヤノン株式会社 Mass spectrometry
US8921599B2 (en) 2010-10-27 2014-12-30 Emory University Processes for forming amide bonds and compositions related thereto

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE8300693L (en) * 1983-02-09 1984-08-10 Sven Lofdahl SET TO MAKE AND ISOLATE PROTEINS AND POLYPEPTIDES, AND A HYBRID VECTOR FOR THIS
GB8308483D0 (en) * 1983-03-28 1983-05-05 Health Lab Service Board Secretion of gene products
JP2526965B2 (en) * 1987-02-24 1996-08-21 武田薬品工業株式会社 Muteins, DNAs and their uses
JP2599591B2 (en) * 1987-04-28 1997-04-09 キヤノン株式会社 Electron emission device characteristics measurement device
US5426036A (en) * 1987-05-05 1995-06-20 Hoechst Aktiengesellschaft Processes for the preparation of foreign proteins in streptomycetes
EP0293158A3 (en) * 1987-05-26 1990-05-09 Merck & Co. Inc. Parathyroid hormone antagonists
DE3725320A1 (en) 1987-07-30 1989-02-09 Biotechnolog Forschung Gmbh EXPRESSION VECTORS AND METHOD THEREFORE FOR THE EXTRACTION OF CRO / SS GALACTOSIDASE / PTH FUSION PROTEINS AND OF PTH
DE69001248T2 (en) * 1989-05-04 1993-10-14 Genentech Inc METHOD AND COMPOSITIONS FOR INSULATING HUMAN RELAXIN.
EP0499990B1 (en) 1991-02-19 1996-05-15 Takeda Chemical Industries, Ltd. Method for producing cysteine-free peptides

Also Published As

Publication number Publication date
ES2087323T3 (en) 1996-07-16
EP0499990A3 (en) 1993-03-17
EP0499990B1 (en) 1996-05-15
EP0499990A2 (en) 1992-08-26
ATE138102T1 (en) 1996-06-15
CA2061382C (en) 2003-02-18
DE69210645D1 (en) 1996-06-20
KR920016463A (en) 1992-09-24
US6287806B1 (en) 2001-09-11
KR100249534B1 (en) 2000-03-15
DE69210645T2 (en) 1996-11-28
US5861284A (en) 1999-01-19

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