CA2071594A1 - Methods for nucleic acid amplification - Google Patents

Methods for nucleic acid amplification

Info

Publication number
CA2071594A1
CA2071594A1 CA002071594A CA2071594A CA2071594A1 CA 2071594 A1 CA2071594 A1 CA 2071594A1 CA 002071594 A CA002071594 A CA 002071594A CA 2071594 A CA2071594 A CA 2071594A CA 2071594 A1 CA2071594 A1 CA 2071594A1
Authority
CA
Canada
Prior art keywords
methods
amplification
nucleic acid
acid amplification
primers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002071594A
Other languages
French (fr)
Other versions
CA2071594C (en
Inventor
Henry A. Erlich
Russell G. Higuchi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2071594A1 publication Critical patent/CA2071594A1/en
Application granted granted Critical
Publication of CA2071594C publication Critical patent/CA2071594C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis

Abstract

Methods are provided for enhanced specificity and sensitivity of nucleic acid amplification. The methods are simplified nested amplification procedures wherein both inner and outer primer pairs are present in the amplification reaction mixture. According to the methods, the thermocycling profile, as well as the sequences, length, and concentration of amplification primers, is modified to regulate which primers are annealed and extended on the target during any particular amplification cycle. The methods described are particularly suitable in PCR amplifications and have numerous applications in molecular biology, medical diagnostics and forensics.
CA002071594A 1991-06-20 1992-06-18 Methods for nucleic acid amplification Expired - Lifetime CA2071594C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US71857691A 1991-06-20 1991-06-20
US718,576 1991-06-20

Publications (2)

Publication Number Publication Date
CA2071594A1 true CA2071594A1 (en) 1992-12-21
CA2071594C CA2071594C (en) 2003-06-17

Family

ID=24886600

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002071594A Expired - Lifetime CA2071594C (en) 1991-06-20 1992-06-18 Methods for nucleic acid amplification

Country Status (9)

Country Link
US (1) US5314809A (en)
EP (1) EP0519338B1 (en)
JP (1) JPH0681600B2 (en)
CA (1) CA2071594C (en)
DE (1) DE69213112T2 (en)
DK (1) DK0519338T3 (en)
ES (1) ES2091976T3 (en)
GR (1) GR3021832T3 (en)
IE (1) IE922008A1 (en)

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Also Published As

Publication number Publication date
DE69213112D1 (en) 1996-10-02
JPH05292968A (en) 1993-11-09
IE922008A1 (en) 1992-12-30
DK0519338T3 (en) 1996-10-28
EP0519338A1 (en) 1992-12-23
US5314809A (en) 1994-05-24
JPH0681600B2 (en) 1994-10-19
EP0519338B1 (en) 1996-08-28
CA2071594C (en) 2003-06-17
GR3021832T3 (en) 1997-02-28
ES2091976T3 (en) 1996-11-16
DE69213112T2 (en) 1997-04-03

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