CA2071904A1 - Antibodies to ligand analogues and their utility in ligand-receptor assays - Google Patents

Antibodies to ligand analogues and their utility in ligand-receptor assays

Info

Publication number
CA2071904A1
CA2071904A1 CA002071904A CA2071904A CA2071904A1 CA 2071904 A1 CA2071904 A1 CA 2071904A1 CA 002071904 A CA002071904 A CA 002071904A CA 2071904 A CA2071904 A CA 2071904A CA 2071904 A1 CA2071904 A1 CA 2071904A1
Authority
CA
Canada
Prior art keywords
ligand
antibodies
analogue
free
utility
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002071904A
Other languages
French (fr)
Other versions
CA2071904C (en
Inventor
Gunars Edwin Valkirs
Kenneth Francis Buechler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Alere San Diego Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2071904A1 publication Critical patent/CA2071904A1/en
Application granted granted Critical
Publication of CA2071904C publication Critical patent/CA2071904C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
    • G01N33/539—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody involving precipitating reagent, e.g. ammonium sulfate
    • G01N33/541—Double or second antibody, i.e. precipitating antibody
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/97—Test strip or test slide
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/822—Identified hapten
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/824—Immunological separation techniques

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

Means for the detection of free ligand analogue conjugate in fluids from competitive ligand-receptor assay processes. Li-gand analogue antibodies that bind the ligand analogue conjugate with substantially greater affinity than their affinity for target ligand are selected and used in competitive ligand-receptor assay processes to bind the free fraction of the ligand analogue conju-gate. This means permits the detection of the free fraction of ligand analogue conjugate even in the presence of substantially high-er concentrations of free target ligand. For the purposes of the present invention, ligand analogue antibodies are antibodies that exhibit at least 100 X greater affinity for the ligand analogue conjugate compared to their affinity for the target ligand.
CA002071904A 1990-09-14 1991-09-04 Antibodies to ligand analogues and their utility in ligand-receptor assays Expired - Lifetime CA2071904C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US583,046 1990-09-14
US07/583,046 US5143852A (en) 1990-09-14 1990-09-14 Antibodies to ligand analogues and their utility in ligand-receptor assays
PCT/US1991/006325 WO1992005283A1 (en) 1990-09-14 1991-09-04 Antibodies to ligand analogues and their utility in ligand-receptor assays

Publications (2)

Publication Number Publication Date
CA2071904A1 true CA2071904A1 (en) 1992-03-15
CA2071904C CA2071904C (en) 2003-02-18

Family

ID=24331471

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002071904A Expired - Lifetime CA2071904C (en) 1990-09-14 1991-09-04 Antibodies to ligand analogues and their utility in ligand-receptor assays

Country Status (11)

Country Link
US (1) US5143852A (en)
EP (1) EP0475783B1 (en)
JP (1) JPH05501922A (en)
AT (1) ATE187250T1 (en)
AU (1) AU8536191A (en)
CA (1) CA2071904C (en)
DE (1) DE69131811T2 (en)
ES (1) ES2141086T3 (en)
IE (1) IE913243A1 (en)
PT (1) PT98951B (en)
WO (1) WO1992005283A1 (en)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5451504A (en) * 1991-07-29 1995-09-19 Serex, Inc. Method and device for detecting the presence of analyte in a sample
US5233042A (en) * 1991-12-16 1993-08-03 Biosite Diagnostics, Inc. Cocaine derivatives
US5283344A (en) * 1993-03-10 1994-02-01 Abbott Laboratories Coupling method using selective amination of maleimide
US6653066B1 (en) 1994-06-17 2003-11-25 Trinity Biotech Device and method for detecting polyvalent substances
DE19504198A1 (en) * 1995-02-09 1996-08-14 Behringwerke Ag Competitive immunoassay using complex analyte derivatives
US5795725A (en) * 1995-04-18 1998-08-18 Biosite Diagnostics Incorporated Methods for the assay of troponin I and T and selection of antibodies for use in immunoassays
US6627404B1 (en) 1995-04-18 2003-09-30 Biosite, Inc. Methods for improving the recovery of troponin I and T in membranes, filters and vessels
US6991907B1 (en) 1995-04-18 2006-01-31 Biosite, Inc. Methods for the assay of troponin I and T and complexes of troponin I and T and selection of antibodies for use in immunoassays
EP0835446B1 (en) 1995-06-26 2003-04-09 Perseptive Biosystems, Inc. High speed, automated, continuous flow, multi-dimensional molecular selection and analysis
US6156521A (en) * 1997-12-19 2000-12-05 Biosite Diagnostics, Inc. Methods for the recovery and measurement of troponin complexes
US5817770A (en) 1997-03-21 1998-10-06 Drug Abuse Sciences, Inc. Cocaethylene immunogens and antibodies
US6830731B1 (en) * 1998-01-05 2004-12-14 Biosite, Inc. Immunoassay fluorometer
US6392894B1 (en) 1998-01-05 2002-05-21 Biosite Incorporated Media carrier for an assay device
US7297554B2 (en) * 1998-11-18 2007-11-20 Microdiagnostics, Inc. Immunoassay system
EP1184666A3 (en) * 2000-08-21 2002-04-10 Roche Diagnostics GmbH Auxiliary haptens and haptenylated agents in displacement immunoassays
EP1395831A2 (en) * 2001-04-19 2004-03-10 NMI Naturwissenschaftliches und Medizinisches Institut an der Universität Tübingen Method for the relative determination of physicochemical properties
CN101021526B (en) 2007-03-16 2012-01-25 艾博生物医药(杭州)有限公司 Apparatus and method for visual display of detecting result
US8956859B1 (en) 2010-08-13 2015-02-17 Aviex Technologies Llc Compositions and methods for determining successful immunization by one or more vaccines
WO2014164594A1 (en) 2013-03-11 2014-10-09 Meso Scale Technologies, Llc. Improved methods for conducting multiplexed assays
GB201403815D0 (en) * 2014-03-04 2014-04-16 Mologic Ltd Assay

Family Cites Families (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3654090A (en) * 1968-09-24 1972-04-04 Organon Method for the determination of antigens and antibodies
US4203802A (en) * 1971-05-14 1980-05-20 Syva Company Inhibitable enzyme amplification assay
US3817837A (en) * 1971-05-14 1974-06-18 Syva Corp Enzyme amplification assay
US3884898A (en) * 1972-08-18 1975-05-20 Syva Co Normorphine derivatives bonded to proteins
US3878187A (en) * 1972-09-11 1975-04-15 Syva Co Polypeptide derivatives of amphetamine and analogs for immunoassays
US4108972A (en) * 1974-03-15 1978-08-22 Dreyer William J Immunological reagent employing radioactive and other tracers
US4048298A (en) * 1975-02-25 1977-09-13 Rohm And Haas Company Solid phase double-antibody radioimmunoassay procedure
US4200690A (en) * 1976-12-16 1980-04-29 Millipore Corporation Immunoassay with membrane immobilized antibody
US4233402A (en) * 1978-04-05 1980-11-11 Syva Company Reagents and method employing channeling
JPS5510590A (en) * 1978-05-04 1980-01-25 Wellcome Found Enzyme immunity quantity analysis
US4246339A (en) * 1978-11-01 1981-01-20 Millipore Corporation Test device
US4281065A (en) * 1979-01-25 1981-07-28 Syva Company Phencyclidine conjugates to antigenic proteins and enzymes
DE2923583A1 (en) * 1979-06-11 1980-12-18 Max Planck Gesellschaft METHOD FOR THE IMMUNOLOGICAL DETERMINATION OF BASAL MEMBRANE MATERIAL AND NEW BASAL MEMBRANE FRAGMENTS SUITABLE FOR THIS
US4391904A (en) * 1979-12-26 1983-07-05 Syva Company Test strip kits in immunoassays and compositions therein
US4366241A (en) * 1980-08-07 1982-12-28 Syva Company Concentrating zone method in heterogeneous immunoassays
US4425438A (en) * 1981-03-13 1984-01-10 Bauman David S Assay method and device
US4446232A (en) * 1981-10-13 1984-05-01 Liotta Lance A Enzyme immunoassay with two-zoned device having bound antigens
US4506009A (en) * 1982-03-30 1985-03-19 University Of California Heterogeneous immunoassay method
GB8317124D0 (en) * 1983-06-23 1983-07-27 Ekins R P Free ligand assay
US4552839A (en) * 1983-08-01 1985-11-12 Syntex (U.S.A.) Inc. Determination of analytes in particle-containing medium
GB8331514D0 (en) * 1983-11-25 1984-01-04 Janssen Pharmaceutica Nv Visualization method
US4703017C1 (en) * 1984-02-14 2001-12-04 Becton Dickinson Co Solid phase assay with visual readout
US4865997A (en) * 1984-02-23 1989-09-12 Becton Dickinson And Company Assay for ligands by separating bound and free tracer from sample
US4632901A (en) * 1984-05-11 1986-12-30 Hybritech Incorporated Method and apparatus for immunoassays
EP0219499A1 (en) * 1984-07-03 1987-04-29 Baralaba Pty. Ltd. Fuel injector
US4745075A (en) * 1984-09-06 1988-05-17 Burroughs Wellcome Co. Diagnostic test methods
US4740468A (en) * 1985-02-14 1988-04-26 Syntex (U.S.A.) Inc. Concentrating immunochemical test device and method
CA1272127A (en) * 1985-04-04 1990-07-31 Hybritech Incorporated Solid phase system for use in ligand-receptor assays
JPS62502495A (en) 1985-04-10 1987-09-24 イムニコン・コ−ポレ−ション Direct homogeneity test
US4803170A (en) 1985-05-09 1989-02-07 Ultra Diagnostics Corporation Competitive immunoassay method, device and test kit
US4670381A (en) * 1985-07-19 1987-06-02 Eastman Kodak Company Heterogeneous immunoassay utilizing horizontal separation in an analytical element
US4806311A (en) * 1985-08-28 1989-02-21 Miles Inc. Multizone analytical element having labeled reagent concentration zone
DE3624464A1 (en) * 1986-07-19 1988-01-28 Boehringer Mannheim Gmbh METHOD FOR DETECTING AN ANALYTIC AND MEANS SUITABLE FOR THIS
US4963468A (en) * 1986-09-05 1990-10-16 Syntex (U.S.A.) Inc. Immunoseparating strip
US4959307A (en) * 1986-09-05 1990-09-25 Syntex (U.S.A.) Inc. Immunoseparating strip
EP0271204A3 (en) * 1986-11-07 1989-10-25 Syntex (U.S.A.) Inc. Immunochromatographic method and device
US5028535A (en) * 1989-01-10 1991-07-02 Biosite Diagnostics, Inc. Threshold ligand-receptor assay

Also Published As

Publication number Publication date
IE913243A1 (en) 1992-02-25
PT98951B (en) 1999-02-26
EP0475783B1 (en) 1999-12-01
EP0475783A1 (en) 1992-03-18
PT98951A (en) 1994-01-31
ATE187250T1 (en) 1999-12-15
US5143852A (en) 1992-09-01
AU8536191A (en) 1992-04-15
ES2141086T3 (en) 2000-03-16
CA2071904C (en) 2003-02-18
JPH05501922A (en) 1993-04-08
WO1992005283A1 (en) 1992-04-02
DE69131811D1 (en) 2000-01-05
DE69131811T2 (en) 2000-03-16

Similar Documents

Publication Publication Date Title
CA2071904A1 (en) Antibodies to ligand analogues and their utility in ligand-receptor assays
EP1376130A3 (en) Assay device with two opposable members
CA2178642A1 (en) Metal ion-ligand coordination complexes, antibodies directed thereto, and assays using such antibodies
CA2064915A1 (en) Tumour necrosis factor binding ligands
ATE177841T1 (en) CROSSTALK OR CROSSTALK INHIBITORS AND THEIR USE
CA2167275A1 (en) Lyophilized ligand-receptor complexes for assays and sensors
CA2195556A1 (en) Polyspecific immunoconjugates and antibody composites for targeting the multidrug resistant phenotype
EP0375422A3 (en) Composition and methods for determining the presence of amphetamines in a sample suspected of containing amphetamine and/or methamphetamine
TW218037B (en)
CA2032907A1 (en) Hepatitis c assay
CA2177362A1 (en) Sensor device for sandwich assay
CA2184195A1 (en) Screening method for identifying ligands for target proteins
EP0468585A3 (en) Biologically active reagent, analytical element and methods for use of the reagent
ATE120233T1 (en) MONOCLONAL ANTIBODIES, B-SPECIFIC FOR APOLIPOPROTEIN, PRODUCED BY TWO HYBRIDOMES.
CA2008304A1 (en) Assay for bone alkaline phosphatase
CA2045475A1 (en) Blood-coagulation factor xiia .beta. monoclonal antibody and immunoassay
WO2003046140A3 (en) Immunoassay and kit for an early and simulataneous detection of biochemical markers in a patient's sample
CA2207505A1 (en) Neurological drug screens
CA2095245A1 (en) Method of assay
EP1591790A3 (en) Method and reagents for determining protein S.
CA2058175A1 (en) Methods for determination of an analyte
CA2075695A1 (en) Monoclonal antibodies to sulfonated polymers
DE68919294T2 (en) Confirmatory evidence of Chlamydia trachomatis.
CA2080408A1 (en) Artificial standard and control sera, processes for the preparation thereof and the use thereof
CA2081879A1 (en) Monoclonal antibodies to polyacrylate polymers

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry