CA2077482C - Hypoallergenic products from natural and/or synthetic components and process of making - Google Patents
Hypoallergenic products from natural and/or synthetic components and process of making Download PDFInfo
- Publication number
- CA2077482C CA2077482C CA002077482A CA2077482A CA2077482C CA 2077482 C CA2077482 C CA 2077482C CA 002077482 A CA002077482 A CA 002077482A CA 2077482 A CA2077482 A CA 2077482A CA 2077482 C CA2077482 C CA 2077482C
- Authority
- CA
- Canada
- Prior art keywords
- protein
- milk
- hypoallergenic
- component
- product
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/14—Milk preparations; Milk powder or milk powder preparations in which the chemical composition of the milk is modified by non-chemical treatment
- A23C9/142—Milk preparations; Milk powder or milk powder preparations in which the chemical composition of the milk is modified by non-chemical treatment by dialysis, reverse osmosis or ultrafiltration
- A23C9/1422—Milk preparations; Milk powder or milk powder preparations in which the chemical composition of the milk is modified by non-chemical treatment by dialysis, reverse osmosis or ultrafiltration by ultrafiltration, microfiltration or diafiltration of milk, e.g. for separating protein and lactose; Treatment of the UF permeate
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C11/00—Milk substitutes, e.g. coffee whitener compositions
- A23C11/02—Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins
- A23C11/06—Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins containing non-milk proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/14—Milk preparations; Milk powder or milk powder preparations in which the chemical composition of the milk is modified by non-chemical treatment
- A23C9/142—Milk preparations; Milk powder or milk powder preparations in which the chemical composition of the milk is modified by non-chemical treatment by dialysis, reverse osmosis or ultrafiltration
- A23C9/1427—Milk preparations; Milk powder or milk powder preparations in which the chemical composition of the milk is modified by non-chemical treatment by dialysis, reverse osmosis or ultrafiltration by dialysis, reverse osmosis or hyperfiltration, e.g. for concentrating or desalting
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/15—Reconstituted or recombined milk products containing neither non-milk fat nor non-milk proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/16—Inorganic salts, minerals or trace elements
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S426/00—Food or edible material: processes, compositions, and products
- Y10S426/801—Pediatric
Abstract
A hypoallergenic milk which has the flavor and smell of natural whole mammalian milk is disclosed herein. The hypoallergenic milk is formed by combining a m~.xture of mineral salts approximately the mineral content of milk, carbohydrate and hypoallergenic protein. Hypoallergenic fat, vitamins and other components are optionally added to meet the minimum daily nutritional requirements for milk. The ingredients are ultrafiltered either separately or in combination as needed to remove allergenic molecules.
Description
/lcw _1_ ~~~r~~~7~
R~CFOALLERGEl~lIC M~hIC PRODU~Tt3 FROM YQATURhL, AA1DlgR 8'YNT~IETIC CONIFOI61E~1T~ ~1D PROUESE DF M2~kC~lNG
Field of the Tnvention A hypoallergenic milk made from the synthetic equivalent of milk mineral salts is disclosed herein.
The hypoallergenic milk has the flavor and smell of whole natural milk, but lacks the component which causes allergic reactions. The hypoallergenic milk has the favorable features of cow°s milk, but lacks foreign animal protein, and therefore may thus be regarded as "humanized" cow°s milk.
background of the Invention Many persons suffer from various allergies, many of which are caused by ingesting food containing allergens.
Although the biochemistry of allergic reactions is not precisely understood, it is believed that the allergens cause, upon ingestion or other contact with the body, a specific reagin (or skin sensitizing antibody) to be formed in the bloodstream. The ability to produce reagins, chemically identified as IgE, in response to a given allergen is thought to be an inherited characteristic that differentiates an allergic person from a non-allergic person. The specificity of the allergen-reagin reaction and its dependence on molecular configuration of the allergen and reagin is similar to the antigen-antibody reaction. The degree of sensitization is dependent upon the extent of exposure /lcw to or ingestion of the allergen. Tn this respect, the allergen molecule, which is often a protein, may be regarded as a "key" which exactly fits the corresponding structural shape of the reagin molecule which may be likened to a "lock". When the key is inserted into the lock, an allergic reaction results.
Different materials contain different allergens, Not all persons may have the reagin with which a par-ticular allergen can react. Therefore, some persons are not allergic to particular materials. However, when a particular reagin reacts with a specific allergen, an allergic reaction results in any number or type of symp-toms. Allergic reactions range from very mild symptoms to death. For example, symptoms, both mild and severe, include skin rashes (allergic eczema and urticaria), dermal symptoms, respiratory symptoms (including allergic rhinitis and bronchial asthma), gastrointestinal symptoms, and migraine. Violent illnesses have been known to include shock-like reactions, vascular collapse and allergic anaphylaxis.
Many allergists have recognized that milk contains proteins which are allergens. The allergens of cow's milk frequently cause the formation of reagins (IgE) in many persons. Thus, many persons, including both adults and children, are allergic to cow°s milk.
Milk is very frequently used in popular food products. It is used not only in cooking arid baking, but it is included in hidden ways as well. For example, casein, caseinate milk solids, whey, whey solids, and lactalbumin are milk products which are components of cookies, cheeses, chocolate (in the form of milk choco-late) , ice cream, butter and may be used as flavoring for other food products, such as breakfast cereals, hot and cold beverages, and desserts. These products can also be found in gravies, breading, whole, dry or evaporated milk, yogurt, sherbet, breads, waffles, creamed vegetables, mashed potatoes, pudding, creamer or any i diverse products such as hot dogs or spaghetti.
Milk products, which are marketed today as hypo-allergenic milk, are neither uniformly hypoallergenic to all patients, nor made from cow's milk. For example, heat processed milk, in which albumin is denatured, is of modest benefit to only a limited number of patients.
A hypoallergenic vegetable soybean milk formulation devised in China has an objectionable smell and after taste. Hypoallergenic milk produced by the acid process which imitates the stomach's digestive process by utilizing hydrochloric acid to break up proteins, e.g.
casein, has an objectionable smell and taste.
Accordingly, there is a need for a hypoallergenic milk which also has the taste and smell of cow's milk.
U.S. Patent No. 4,293,571 discloses a process for the purification of purified protein hydrolysate. In this process, an aqueous solution of protein is subjected to hydrolysis, then is heat treated to denature the protein. The heat-treated material is then ultrafiltered to eliminate protein.
U.S. Patent No. 4,402,938 discloses a food and method for making the same from colostrum and milk. In this process, the udder of an ungulate is stimulated with an antigen-like material so that the food factor of the whey is enhanced. The enhanced milk is subsequently ultrafiltered. The retentate is discarded and the permeate is saved. Preservatives are added to the milk/colostrum prior to ultrafiltration.
The inventor's cQmmpnly assigned U,S. Patent Nos. 4,954,361 and 5 064,674 , filed August 3, 1990 disclose hypoallergenic milk products from ultrafiltration of cow's milk. The resulting good fasting products are substantially free of cow's milk protein and fat. While these products represent an advance in the state of the art, they are derived from whole milk or fractions thereof . What is needed is a product which has the good taste and nutritional value /lcw _4_ ~~~p~~~iY' of milk-based products, but which may be prepared from synthetic sources. According to the invention hereinafter described, the mineral salts, carbohydrate and protein do not necessarily have to be derived from milk.
summary pf the Inv~nta.~xa A hypoallergenic milk product is provided comprising (i) a mineral salt component comprising a mixture of mineral salts approximating the mineral content of natural milk, (ii) a carbohydrate component comprising one or more carbohydrates and (iii) a hypoallergenic protein component which may advantageously comprise hypo-allergenic protein per se, amino acids, polypeptides having a molecular weight of not more than about 5.0 kDa, or a combination thereof.
The hypoallergenic milk product is prepared by the steps of forming an aqueous mixture of the mineral salt component, carbohydrates, and hypoallergenic protein com-ponent. One or more of the separate components, and/or any combination thereof, are filtered through a filtration membrane, which will only allow molecules with a molecular weight of less than or equal to about 5 kDa to pass therethrough. The permeate is thereafter collected and utilized. The product may be optionally supplemented with hypoallergenic fat, vitamins and addi tional minerals. The filtration steps) are necessary for any components which may be derived from milk sources, e.g., whey-derived components, or components which may otherwise contain allergenic protein.
It is an object of this invention to produce a new and useful hypoallergenic food product from a synthetic or natural mixture of the mineral salts found in natural mammalian milk, and from non-milk or milk-derived car bohydrates or mixtures thereof.
It is another object of this invention to obtain the good taste of natural whole or skim milk.
/lcw It is an object of the invention to provide a hypoallergenic milk product which retains the nutritional content of natural milk.
It is an object of the invention to provide a 5 hypoallergenic milk product which may serve as a vehicle for delivery of specialized nutritional products.
It is an object of the invention to provide a hypoallergenic milk product at low production cost, pos sessing the excellent taste of farm fresh milk, yet free of animal protein.
~etail.ed ~escrxgtion of th~ Tnvention The hypoallergenic milk product disclosed herein after is formulated upon the fact that protein contained in natural milk is the source of allergens that react with reagins to produce allergic reactions. Similar to the antigen-antibody reaction, it is believed that the allergen molecules in cow°s milk, which usually are proteins, have a specific structure which acts as a "key", while the reagins have a corresponding structure which acts as a "lock". While this is the theory upon which the hypoallergenic milk is based, this theory is not meant to be limiting upon the embodiments disclosed hereinafter.
A mixture of mineral salts wYiich will provide the inorganic content of mammalian milk, forms the basis of the present product. The mineral salt mixture, typically in the form of an aqueous solution thereof, is combined with carbohydrate, and a hypoallergenic protein component in amounts approximating the mineral salt, carbohydrate and protean content of natural milk. Hypoallergenic fat is optionally added. Further optional ingredients include additional minerals and trace minerals, vitamins and flavoring agents. Preferably, the hypoallergenic milk product contains, on a weight percentage basis, about 0.7-0.9% mineral salts, about 3 - 4% carbohydrate, about 1-4% hypoallergenic protein component, about 0-4%
/lcw -g_ hypoallergenic fat, the balance comprising water and minor optional ingredients.
The mineral salt mixture may be prepared syntheti cally, by combining mineral salts which will provide a solution approximating the mineral content of natural milk, in the approximate proportions found in milk.
Mineral salts useful for this purpose include the follow-ing: potassium phosphate (monobasic), potassium citrate, sodium citrate, potassium sulfate, calcium chloride, magnesium citrate ~monobasic), potassium carbonate, and potassium chloride. They are combined and dissolved in the appropriate volume of water to form a mineral salt solution which is the basis for the present hypoallergenic synthetic milk product. While the above salts represent the more significant mineral salts of cow's milk, "mineral salt mixture" as used herein shall mean a mixture of substantially the maj ority of the above salts, or equivalent salts providing the mineral content of natural milk, plus any additional organic or inorganic salts which may be added without prejudice to the taste of the resulting product. The mineral salt component thus prepared is of course hypoallergenic since it contains no protein.
Whey is the component of milk which remains after all or a substantial portion of the fat and casein con tained are removed. Various fractions or variants of whey are known, such as, for example, sweet whey permeate (i.e., permeate of whey prepared by crude filtration), dried sweet whey, demineralized whey, delactose whey, whey protein concentrate, and the like. Delactosed whey, that is, whey from which the lactose has been removed, may be advantageously utilized as a natural source of the milk mineral salt component of the present hypoallergenic milk product. Delactosed whey is commercially available in liquid or powder form.
The carbohydrate component may comprise any suitable monosaccharide, disaccharide, polysaccharide or com-/lcw bination thereof. Such sugars include, but are not necessary limited to lactose, galactose, glucose, sucrose, fructose, maltose, maltodextrins and mixtures thereof. The preferred sugar is lactose, which is derived from milk. However, care should be taken in processing carbohydrate from commercial sources, particulary the milk sugars such as lactose, before inclusion in the hypoallergenic milk product. Lactose purified from cow's milk or whey contains as much as 0.01 wt.% milk protein, which becomes trapped in the lactose crystals during the purification process. carbohydrate derived from non-milk sources may also contain small but significant amounts of allergies. For example, sugars derived from corn syrup or sugar cane may contain 0.1%
protein. Thus the carbohydrate component of the invention is advantageously ultrafiltered before use as hereinafter described, or alternatively, the complete milk product as constructed from its component parts is ultrafiltered. Ultrafiltration, as hereinafter described, will remove any trace amounts of hypoaller-genic protein which may be contained in the carbohydrate component.
The hypoallergenic protein component may comprise hypoallergenic protein per se, such as protein from cereal or vegetable sources. Alternatively, or additionally, it may comprise free amino acids, or poly peptides of animal source, provided tre polypeptides are not larger than about 5 kDa, preferably not larger than about 3.5 kDa, more preferably not larger than about 2 kDa, and most preferably not more than about 1 kDa.
Sources of hypoallergenic protein include, but are not limited to: oat cereal (which has a high protein level of about 18%): rice cereal: barley cereal; or any other food source having a low allergenicity and ample protein content. Vegetable sources of protein may also be used, so long as they have a low allergenic potential.
Vegetable sources of low allergenic protein include, for /1Cw _g_ example, potato and soy isolate. Combinations of the foregoing proteins may also be used.
Oat cereal, for example oatmeal, is preferred because it not only enhances the protein content, but also adds to the taste of the resulting product. The oat cereal is used as a very finely ground flour, to facilitate dissolution into the permeate. About 5 to 10 grams of the very finely ground and sieved cereal flour is added to about 100 cc of product. The resulting 20 mixture has a protein content of about 0.9 to 1.8~ by weight, which is similar to human breast milk.
When cereals are used, protein say isolate may else be added to enrich the lysine amino acid value of the cereal. Additionally, the protein may be supplemented with, among other things, methianine, cystine, and iodine to meet the minimum daily requirements.
Protein soy isolate is preferred for use in hypoal-lergenic milk which is intended far infants, who require a single source of protein, or children and adolescents with important growth factor requirements. Cereal hypoallergenic protein sources can be used in the hypoallergenic milk for adults. For example, if a multiple source of protein is desired, any combination of hypoallergenic protein sources may be used.
Tn lieu of, or in addition ta, supplementation with hypoallergenic protein, the product may be supplemented with amino acids, short chain polypeptides, or a combina~
tion of amino acids and short chain polypeptides. By "short chain polypeptide" is meant a polypeptide having a molecular weight of not more than about 5 kDa, preferably not mare than about 1.5 kDa, mare preferably not more than about 1 kDa. Free amino acids and short chain polypeptides are hypoallergenic regardless of source, and therefore will net contribute to the allergenicity of the milk product. Preferably, the amino acids comprise a mixture of amino acids, most preferably a mixture containing at least the nine amino acids which /lcw -are essential to the human diet:
Threonine Valine Phenylalanine Methionine Isoleucine Histidine Lysine Leucine Tryptophan The short chain polypeptides may comprise individual polypeptides or a mixture of polypeptides. The short chain polypeptides and amino acids may be obtained by appropriate hydrolysis of any suitable polypeptides or proteins. Preferably, they are obtained from milk pro-teins, so that the reconstituted hypoallergenic milk product of the invention maintains a portion of the protein nutritional content of whole milk. Hydrolysates of milk proteins are commercially available. For example, a series of hydrolysates are produced by Deltown Chemurgic Corporation, Fraser, New York, under the trademark "DELLAC'°. "DELLAC'° CE80PS is a highly hydrolyzed pancreatic digest of casein. "DELLAC'° LE80PS
is a hydrolyzed pancreatic digest of another milk protein, lactalbumin. High-performance liquid chromatography indicates that these products are free of polypeptides having a molecular weight of greater than about 1.5 kDa. Hydrolysates of non-milk proteins may also be employed, e.g. "DELLAC°' SE50M, which is a papaic digest of soy flour. Other milk protein hydrolysates are sold under the trademark '°ALATAL" by New Zealand Milk Products, Inc., Petaluma, CA (a division of the New Zealand Dairy Board, Wellington, New Zealand).
Each of the aforementioned products may be advantageously utilized in the practice of the invention since they are either free of allergenic milk protein, or are at least free of any milk-derived polypeptides large enough to be considered allergenic.
The protein component is optionally ultrafiltered prior to addition to the other product components . Where the protein component comprises vegetable protein, amino /lcw ~~~~~J~
acids, or short chain polypeptides, filtration is probably not necessary, as these materials are hypoallergenic. However, where filtration of the protein component is practiced, either separately or by filtration of the completed milk product, it may then be feasible to utilize as a source of short chain polypeptides milk protein hydrolysates which may include some polypeptide spacies large enough to be considered hyperallergenic. These larger species will be removed by the filtration step. Preferred such milk protein hydrosylates include °'ALATAL'° 817 and °'DELLAC" LE80GF, which are enzymatic digests of lactalbumin. The latter product consists of 80% by weight protein-derived materials, of which 97 wt.% comprises short chain polypeptide and 3 wt.% whole protein. "DELLAC" LE80GF
has an average molecular weight to about 2 kDa.
Hydrolysates of lactalbumin are particularly prefer-red for supplying short chain polypeptides and/or amino acids in the practice of the present invention. Lactal bumin and its hydrolysates contain a relative surplus of the four essential amino acids lysine, methionine, threonine and isoleucine. They can, therefore, be an important supplement to cereal or vegetable protein which is somewhat deficient in these amino acids. Lactalbumin hydrolysates are particularly useful when combined with other protein sources, such as soy isolate or casein hydrolysate, which may be somewhat deficient in the amino acids cystine and methionine.
It should be noted that while the aforementioned refined polypeptide-containing products result from enzyme hydrolysis of single milk proteins, their addition to the permeate prepared according to the present invention does not significantly impact on the taste of the final reconstituted hypoallergenic milk product.
This should be contrasted with the situation where whole milk, before filtration, is treated in situ with hydrolytic enzymes. This form of in situ hydrolysis of 4403-g CN 2 /lcw milk proteins deleteriously impacts on the taste of the resulting product.
The sources of the optional fat component may include deproteinized clear butter and butter oil or butter fat, polyunsaturated and mono- and/or polyunsaturated vegetable oil or fat from milk free margarine sources, sesame, safflower, and the like, or mixtures thereof. The foregoing fats are hypoallergenic.
The fat is optionally added to the mineral salt solution so that the fat content of the final product ranges between 0% and about 4% by weight depending upon whether skim, 1%, 2% or 4% homogenized milk is desired.
Fox adults where atherosclerosis prevention is of great importance, the fat source may comprise about 1/4 to about 1/2% deproteinized butter oil and/or about 1/2 to about 2% low fat polyunsaturated vegetable fat.
Deproteinized hypoallergenic butter for supplement-ing the permeate may be made from commercially available salt-free, sweet 99.99% anhydrous milk fat. The milk fat is melted in boiling water. The resulting butter oil is then removed from the boiling water, such as by pipetting it off the surface of the water. The boiling water results in extreme heat denaturation of protein and also renders the resulting heat-denatured protein insoluble.
The process removes, by dilution and washing of the milk fat with water, any protein which may be contained in the fat as a contaminant. The process may be repeated any number of times to ensure the purity of the resulting butter product.
Vitamins, and further minerals in addition to those present in the mineral salt component, are also optionally added to the protein- and fat-supplemented mineral salt solution. Such vitamins and minerals are added, so that the resulting milk products meet the minimum daily requirement. By way of non-limiting example, the following may be added, based upon one quart of mineral salt solution supplemented with carbohydrate, /lcw hypoallergenic protein and fat: 400 micrograms of water dispersible Vitamin D; 2100 micrograms of water-dispersible Vitamin A; 60 milligrams of Vitamin C
acetate; folic acid; calcium pantothenate: biotin;
pyridoxine; vitamin B3; vitamin K~ (0.1 mg/1) ; vitamin B12 (1.5 mg/1); vitamin E (20 ~l/1); thiamin (0.6o mg/1);
riboflavin (0.6 mg/ml); vitamin B6 (0.4 mg/ml); minerals such as calcium as phosphate, carbonate or triphosphate;
iron as ferrous sulfate; and zinc as zinc sulfate. Other added minerals may advantageously include cupric sulfate, sodium iodide, potassium carbonate; potassium chloride;
and sodium selenite. Preferred amino acids which may be added include L-cysteine, L-tyrosine and L-tryptophan.
The foregoing are exemplary of the vitamins and minerals that may be added to the hypoallergenic milk. Of course, other vitamins and minerals which are known to those of ordinary skill in the art may also be added.
Additives to enhance the flavor and consistency of the hypoallergenic milk may also be added. Exemplary 2o additives includes hypoallergenic bean gum derived from guar gum (for example, about 3.7 to about 4.7 kg of bean gum per 10,000 liters of hypoallergenic milk);
carrageenan; and/or lecithin of hypoallergenic vegetable bean source, such as soy bean (for example, about 24 kg/10,000 liters of hypoallergenic milk) or say-derived emulsifier. Each of these additives imparts a creamy consistency (acts as an emulsifier) to the hypoallergenic milk. Natural vanilla may also be added to enhance the flavor of hypoallergenic milk.
The hypoallergenic protein component, or mineral salt component when derived from a milk product such as delactose whey, or the carbohydrate component when it comprises lactose or other milk-derived sugar, may be ultrafiltered to ensure that no hypoallergenic protein contaminants are included in the hypoallergenic milk product. The components may be filtered separately or in combination with one another.
/lcw -13- ~~~~~~7~'7 a .fi ~) ~,i Accarding to one embodiment, an ultrafiltration mem-brane utilized for filtration is sized to prevent the passage of any substance with a molecular weight greater than 5 kDa. Such excluded substances include, but are not limited to: milk protein; viable or non-viable bacteria; bacterial protein antigen; and milk fat.
Alternately, ultrafiltration membranes which prevent the passage of any substance with a molecular weight greater than 1 or 2 kDa may also be used. Ultrafiltration mem-branes capable of preventing the passage of 1 or 2 kDa molecular weight substances have proportionately smaller pore sizes.
The following contaminating milk proteins which may be present in protein or carbohydrate components derived from milk sources, are trapped by the ultrafiltration membrane (molecular weights are noted in parenthesis)a alpha lactalbumin (14 kDa); kappa casein (23 kDa); alpha S-1 casein; alpha S-2 casein; beta casein (24 kDa); beta lactoglobulin (37 kDa); bovine serum albumin (f>5 kDa);
and immunoglobulins (>100 kDa). These milk proteins are considered allergenic. Beta lactoglobulin is a dimer at pH 6.6.
It has been found that decreasing the sizing of the filter decreases the relative amount of three milk proteins - alpha lactalbumin, beta lactoglobulin and bovine serum albumin. Thus, where 0.27, 0.33 and 0.01 units of these proteins, respectively, are found in products prepared with a 10 kDa membrane (i.e., a filter membrane which excludes molecules having a molecular weight greater than 10 kDa) , products prepared with 5 kDa dalton filters contain 0.03, 0.03 and 0.01 units of these same proteins, respectively. A dialysate product, prepared using a 3.5 kDa dialysis membrane, contains less than 0.01 units of each of these protein species, resulting in a protein-free dialysate, based upon the limits of the electrophoretic method employed to analyze for protein.
/lcw Ultrafiltration membranes having a 3.5 kDa or less molecular weight cut-aff are preferred. Polyether sulfone membranes having a 1 kDa or 2 kDa cut-off are available, for example, from Advanced Membrane Technology, San Diego, CA and Dow Denmark, Naskov, Denmark, respectively. Ultrafiltration membranes made of ceramic materials may also be used.
Ceramic filters have an advantage over synthetic filters. Ceramic filters can be sterilized with live steam so that the chemical agents, such as chlorine, do not have to be used to sterilize the filter. Synthetic filters, on the other hand, cannot be sterilized with live steam, but instead they must be sterilized with chemical agents, for example, a solution of 200 parts per million (p.p.m) chlorine solution may be used to disinfect the membrane. If a chemical agent is used to disinfect the membrane, the chemical agent may be washed from the filter by flushing the filter with two passes of milk.
A pressure gradient is preferably applied across the ultrafiltration membrane to facilitate filtration.
Preferably, the pressure gradient is adjusted to maintain a filter flux of about 24 liters/m~-hour, which is the typical dairy plant filter flux. The filter is advan-tageously first primed with a small amount of product and the permeate discarded, prior to beginning filtration.
Priming of the filter in this manner is believed to be advantageous to filtering efficiency.
The pH of the product during filtration should be within the range of about 2 to about 11. The preferred pH is about 6.6.
The temperature of the product component during ultrafiltration should be within the range of about 4°C
(i.e., about 40°F) to about 66°C (i.e., about 150°F).
Instead of ultrafiltration, any allergenic com-ponents of the milk component may be removed by dialysis.
As is well known, dialysis operates on a principal akin /lcw 1~
to osmosis. Any allergenic protein in the permeate is effectively trapped utilizing a 5 kDa, (preferably a 3.5 kDa, 2 kDa or 1 kDa) ultrafilter or dialysis membrane.
With dialysis, as with ultrafiltration, the permeate that passes through the membrane, i.e. the hypoallergenic component, is saved and utilized. The retentate, i.e., the material which does not pass through the membrane, is discarded or utilized in other commercial applica tions. A dialysis membrane capable of preventing the passage of materials with a molecular weight of 5 kDa may be used. Other membranes, however, could be used so long as the hyperallergenic component is excluded from the permeate.
Preservatives such as phenol, parabens etc. are preferably not added to the hypoallergenic milk product.
The product may be supplemented, as discussed above, while in liquid form. Alternatively, or additionally, it may be freeze dried in any conventional manner, then reconstituted with liquid supplements at a later time.
After the hypoallergenic protein component, car-bohydrate and optional fats, vitamins and additives to enhance flavor and consistency have been added to the mineral salt solution, the resulting hypoallergenic milk is preferably blended in an emulsifying and diffusing ap-paratus operating at between about 2,500 and about 3,500 r.p.m., to ensure thorough mixing. The blended hypoallergenic milk is then homogenized at a pressure ranging from about 138 to about 276 Bar (i.e., about 2,000 to about 4,000 P.S.T.), pasteurized at about 77°C
(170°F) for about 30 minutes, and then flashed sterilized at about 143°C (290°F) for about 12 seconds and packaged into a aseptic containers. Such containers are made of materials which will not leach into the packaged product.
The materials include, but are not limited to, glass, waxed cardboard or metal. Alternatively, the product may be pasteu~.rized before the various supplements have been added.
4403°8 C3~1 2 /lcw The meticulous removal of substantially all aller-genic protein by an ultrafilter or dialysis membrane has superior advantage regarding hypoallergenicity. The product is free of protein as evidenced by the absence of protein bands upon SDS-PAGE, sensitive to the application of 30 nanograms/microliter or greater concentration of protein. Thus, it is understood 'that as used herein, the expression "substantially completely deproteinized°' or "substantially free of milk protein°' in referring to a preparation, means a preparation free of protein bands upon SDS-PAGE sensitive to the presence of protein concentrations of 30 nanograms/microliter or higher.
Where the carbohydrate component comprises lactose, lactase enzyme may be added to the hypoallergenic milk for use by lactose--intolerant individuals. Alterna tively, in lieu of the preferred sugar lactose, other sugars may be utilized, such as glucose, fructose, maltose or maltodextrin.
The hypoallergenic milk may be substituted for milk components in any formulation in which milk components are used. For example, hypoallergenic milk may be used as a beverage or in beverages, or solid food products such as candy, milk chocolate, cookies, cakes, breakfast cereals and the like. The hypoallergenic milk product may also be utilized as a vehicle for the delivery of specialized nutritional products, which might otherwise have an objectionable taste to the patient. Thus, use of the hypoallergenic milk product as a vehicle for offensive-tasting enteral products may obviate the need for introducing such products by stomach tube, which occurs in patients suffering from such diseases as ileitis, colitis, and geriatric nursing home patients.
One non-limiting hypoallergenic milk product according to the present invention suitable for infants contains the following components, based upon 100 ml of product. The amount of each component may be adjusted _17_ rr s.
'~ l~ ~) according to need.
Protein Soy protein isolate 1.8 g Oatmeal protein (optional) 0.9 to 1.8 g ~'at 3.7 g Oarbotaydrate Lactose (or equivalent 4.6 g concentration of sucrose, maltose, glucose or fructose) Minerals Sodium 41 to 49 mg Potassium 140 to 152 mg Calcium 110 to 119 mg Phosphorus 89 to 93 mg Chloride 63 to 65 mg Iron (fortified) 0.05 to 1.2 mg Zinc 0.38 to 0.43 mg Iodine 10 micrograms Amino ~9.cids Methionine 10 micrograms Cystine 10 micrograms Vitamins Vitamin A 210 International Units (water dispersible) ("I.U.") Vitamin C 6.0 mg (as acetate) Vitamin D 42 I.U»
(water dispersible) Vitamin E 1.0 mg Thiamine 0.04 mg Riboflavin 0.14 to 0.16 mg Niacin 0.08 mg Pyridoxine 0.04 to 0.05 mg Vitamin B~2 0.32 micrograms Folic Acid 5.0 micrograms According to another embodiment, a hypoallergenic milk product suitable for infants has the foregoing com-ponents, except that the soy protein isolate, methione and cystine are omitted in favor of a mixture of free amino acids, comprising preferably at least all the essential amino acids, or, alternatively a mixture of short chain polypeptides. Preferably, the infant formula utilizes a mixture of short chain polypeptides derived from milk protein, such as any of the available hydrolysates of milk proteins described above.
The method of the invention is effective in prepar-ing a hypoallergenic milk product wherein the hypoaller-genic protein content of milk is reduced from 3.6o to 0.26, a reduction of more than 90%, and lower, by utilizing a filter capable of retaining 5 kDa molecular weight, more preferably filters capable of retaining even smaller molecules.
The invention will now be described in more detail with reference to the following specific, non-limiting examples:
/lcw -19- ~~r~r~~~
To 10 liters of distilled water, 800 grams of "AhATAL°' 817 milk protein hydrosylate (New Zealand Milk Products) were added, as well as 150 grams of lactose obtained by crystallization of a whey permeate, and 1400 ml of a mixture of milk mineral salts in the form of a delactosed whey permeate. This mixture was purified and deproteinized by passage through a 1 kDa cut-off ultrafiltration membrane ("A.E.S.-1", Advanced Membrane Technology, San Diego, CA) at about 21°C (i.e., about 70°F). Filtration was facilitated by maintaining a pressure gradient of about 15 Bar (i.e., about 211.5 p.s.i.) to provide a filter flux of 24 liters/m2-hour or more. The final ultrafiltered permeate was then trans ferred to screw top containers.
Example 1 was repeated substituting the 2 kDa cut off ultrafiltration membrane ("GR90 2K°°, Dow Denmark, Naskov, Denmark) for the 1 kDa cut-off ultrafiltration used in Example 1. The pressure gradient required was increased to about 15 Bar (i.e., about 211.5 p.s.i.).
The permeate was then transferred to screw top containers.
500 ml of the final ultrafiltered permeate of Example 1 was pasteurized at about 72°C (i.e., about 162°F) for 20 minutes. This heating further serves to denature any remaining trace of protein. The permeate was then supplemented by adding 2.5 ml of cleared butter oil (as prepared according to Example 5 of U.S. Patent 4,954,361). The supplemented permeate was then homogen-ized using a homogenizes operating at 9000 r.p.m. The formulation was then decanted into two 4 ounce glass bottles and refrigerated.
/lcw '20 ~~ ~ ~~~~r EX~MPIaE 9~
500 ml of the final ultrafiltered permeate of Example 2 was pasteurized at about 72°C for 20 minutes.
This heating further serves to denature any remaining traces of protein. The permeate was then supplemented with 2.5 ml of cleared anhydrous butter oil.
EX~1RSPLE 5 To 10 liters of distilled water was added 800 grams of "ALATAL" 817 milk protein hydrosylate. This was deproteinated by passage through a 1 kDa cut-off ultrafiltration membrane ("A.E.S.-1", Advanced Membrane Technology, San Diego, CA) at about 27.°C (i.e., about 70°F). Filtration was facilitated by maintaining a pressure gradient of about 5 Bar (i.e., about 75 p.s.i.) to provide a filter flux of 24 liters/m2-hour or more.
500 ml of the ultrafiltered permeate was then transferred to screw top containers. Twenty grams of sucrose and 3.79 g of a mineral salt solution were further added.
The final p.H. was 6.7. The mineral salt solution is prepared by grinding and mixing the following quantities of mineral salts in grams:
potassium citrate 5 potassium phosphate (monobasic) 15.80 sodium citrate 21.20 potassium sulfate 1.80 calcium chloride 13.20 magnesium citrate (monobasic) 5.02 potassium carbonate 3.00 potassium chloride 10.78 The above mineral salt quantities are sufficient for 20 liters of stock mineral salt solution. For one later of solution, 7.59 grams of the dry blend are weighed out and dissolved in 975-990 ml of distilled water. Tt was not necessary to adjust pH with 1.0-1.5 N KOH or with 4403-8 CId 2 /lcw °21 magnesium oxide.
EXAMPLE S
Example 5 was repeated substituting the 2 kDa cut°
off ultrafiltration membrane ("GR90 2K", Dow Denmark, Naskov, Denmark) for the 1 kDa cut°off ultrafiltration used in Example 16. The pxessure gradient required was about 15 Bar (i.e., about 211.5 p.s.i.). Seven grams of refined powdered dextrose was added to 200 ml of Example 6 instead of sucrose as in Example 5. The permeate was then transferred to screw top containers.
Ex~~LE a 500 ml of the final ultxafiltered and enriched permeate of Example 5 was pasteurized at about 72°C for minutes. The permeate was supplemented with 2.5 ml of cleared butter oil. The enriched permeate was then homogenized using a homogenizer.
500 ml of the final ultrafiltered permeate of Example 6 was pasteurized at about 172°C, supplemented with 2.5 ml of cleared anhydrous butter oil and homogenized.
EXAMPLE ~
To 10 liters of distilled water were added 800 grams of "ALATAL" 817 and 150 g of lactose prepared by crystallization from whey permeate. The mixture was deproteinized and purified by passage through a lkDa cut-off ultrafiltration membrane ('°A.P.S.-1'° Advanced Membrane Technology, San Diego, CA) at about 21°C (about 70°F). Filtration was facilitated by maintaining a pressure gradient of about 15 Bar (about 211.5 PSI) to provide a filter flux of 24 liters/m~-hr or more. 500 ml of the final ultrafiltered permeate was then transferred to screw-top containers. To an 0.5 later /lcw --22-container of the permeate was added 3.79 grams of the mineral salt dry blend of Example 5 and 20.0 grams of glucose in the form of refined corn syrup. 'rhe final formulation contains about 3.5% carbohydrate (lactose +
glucose); a greatly reduced lactose concentration, without necessitating the use of lactase enzyme.
All steps of Example 9 were repeated substituting the 2 kDa cut-off ultrafiltration membrane ("GR90 2K" Dow Denmark Naskov, Denmark) for the 2 kDa cut-off ultrafiltration membrane used in Example 9. The pressure gradient was increased to about 15 Bar (about 211.5 PSI).
The permeate was then transferred to screw-top containers.
EXAP'tPL~ 3.3.
500 ml of the final ultrafiltered permeate of Example 9 was pasteurized at about 72°C for 20 minutes.
The permeate was then supplemented with 2.5 ml of cleared anhydrous butter oil and homogenized as before.
El~lAP~ipLE 9.2 500 ml of the final ultrafiltered permeate of Example 10 was pasteurized at about 72°C, supplemented with 2.5 ml of cleared anhydrous butter oil and homogenized.
In each of Examples 1-12, about 2.0 grams of a commercial, vanilla-flavored oat soy preparation was added to a 100 m1 sample of the resulting permeate for body, flavor and emulsification enhancement. This mixture was then further homogenized and emulsified with a homogenizer/emulsifier operating at 9,000 r.p.m.
4403°8 CN 2 ~lCw --23- ~~~~~8~
ES~PI~LE ~.3 To 10 liters of distilled water was added 800 grams of "ALATAL°' 817 and 1400 ml of delactose whey permeate.
The mixture was then deproteinized by passage through a 3 kDa cut-off ultrafiltration membrane ("A.E.S.-1") at about 21°C. Filtration was facilitated by maintaining a pressure gradient of about 15 Dar (211.5 PSI) to provide a filter flux of 24 liters/m2--hour or more. The final ultrafilter permeate was then transferred to screw top containers. 0.5 liter of the synthetic permeate is then supplemented with 5 g of dextrose. The final formulation contains about 3.4o carbohydrate (lactose -~
glucose); a greatly reduced lactose without necessitating the use of lactase enzyme.
EXAP2~LE 1.~
Example 13 was repeated substituting the 2 kDa cut off ultrafiltration membrane ('°GR 90 2K") for the 1 kDa cut-off ultrafiltration filter used in Example 13. The pressure gradient was increased to 15 Bar.
500 ml of the final ultrafiltered permeate of Example 14 is pasteurized at about 72°C for twenty minu tes, supplemented with 2.5 ml of cleared anhydrous butter oil and homogenized as before.
EXAMPLE 7.6 500 ml of the final ultrafiltered permeate of Example 15 is pasteurized, supplemented with 2.5 ml of cleared anhydrous butter oil and homogenized.
In each of Examples 13 - 16, a 100 ml sample of the resulting permeate is mixed with about 2.0 grams of a commercial, vanilla-flavored oat soy preparation as set out above for body, flavor and emulsificatian enhance-/lcw ment.
The presence of medication utilized to treat milk-producing cows is undesirable in milk for human consump-tion. The ultrafiltration method described herein is believed to effectively reduce the level of veterinary pharmaceuticals contained in fractions of cow°s milk.
Approximately 750 of monocyclic drugs, e.g., penicillin and sulfonamides, which may be present in the milk, are attached to the milk's protein fraction. Approximately 25~ or more of tricyclic compounds, and approximately 50~
of bicyclic compounds, are similarly found attached to the protein fraction. Thus, it may be readily appreciated that removal of milk protein, as in the practice of the present invention, serves also to substantially reduce the level of veterinary medications which may be contained in cow's milk.
Recently, bovine immunodeficiency infection in cows has been reported to result in decreased milk production.
While this viral agent has not been found to be transmis-sible to humans, the ultrafiltration procedure utilized herein excludes viruses and bacteria, which are generally larger than 100 kLla and 1,000 kDa respectively. Thus, ultrafiltration ensures that these disease agents which may be carried in milk protein hydrosylates and/or delactose whey permeates utilized in the practice of the invention, do not infect the resulting hypoallergenic milk product.
The anaphylactic type of allergic reactions may be caused by milk proteins. Many patients, particularly children, have symptoms of recurrent colds, bronchitis, asthmatic bronchitis, asthma, as well as recurrent sinusitis and/or otitis. These symptoms are often relieved by avoidance of milk. These symptoms may actually be caused by viral or bacterial protein present in milk. Where milk components such as milk protein hydrosylates and delactose whey are utilized in the practice of the present invention, the ultrafiltration /lcw -?5-procedure removes protein, as well as bacteria and viruses which may cause these symptoms.
'fhe present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof and, accordingly, reference should be made to the appended claims, rather than to the foregoing specification, as indicating the scope of the invention.
R~CFOALLERGEl~lIC M~hIC PRODU~Tt3 FROM YQATURhL, AA1DlgR 8'YNT~IETIC CONIFOI61E~1T~ ~1D PROUESE DF M2~kC~lNG
Field of the Tnvention A hypoallergenic milk made from the synthetic equivalent of milk mineral salts is disclosed herein.
The hypoallergenic milk has the flavor and smell of whole natural milk, but lacks the component which causes allergic reactions. The hypoallergenic milk has the favorable features of cow°s milk, but lacks foreign animal protein, and therefore may thus be regarded as "humanized" cow°s milk.
background of the Invention Many persons suffer from various allergies, many of which are caused by ingesting food containing allergens.
Although the biochemistry of allergic reactions is not precisely understood, it is believed that the allergens cause, upon ingestion or other contact with the body, a specific reagin (or skin sensitizing antibody) to be formed in the bloodstream. The ability to produce reagins, chemically identified as IgE, in response to a given allergen is thought to be an inherited characteristic that differentiates an allergic person from a non-allergic person. The specificity of the allergen-reagin reaction and its dependence on molecular configuration of the allergen and reagin is similar to the antigen-antibody reaction. The degree of sensitization is dependent upon the extent of exposure /lcw to or ingestion of the allergen. Tn this respect, the allergen molecule, which is often a protein, may be regarded as a "key" which exactly fits the corresponding structural shape of the reagin molecule which may be likened to a "lock". When the key is inserted into the lock, an allergic reaction results.
Different materials contain different allergens, Not all persons may have the reagin with which a par-ticular allergen can react. Therefore, some persons are not allergic to particular materials. However, when a particular reagin reacts with a specific allergen, an allergic reaction results in any number or type of symp-toms. Allergic reactions range from very mild symptoms to death. For example, symptoms, both mild and severe, include skin rashes (allergic eczema and urticaria), dermal symptoms, respiratory symptoms (including allergic rhinitis and bronchial asthma), gastrointestinal symptoms, and migraine. Violent illnesses have been known to include shock-like reactions, vascular collapse and allergic anaphylaxis.
Many allergists have recognized that milk contains proteins which are allergens. The allergens of cow's milk frequently cause the formation of reagins (IgE) in many persons. Thus, many persons, including both adults and children, are allergic to cow°s milk.
Milk is very frequently used in popular food products. It is used not only in cooking arid baking, but it is included in hidden ways as well. For example, casein, caseinate milk solids, whey, whey solids, and lactalbumin are milk products which are components of cookies, cheeses, chocolate (in the form of milk choco-late) , ice cream, butter and may be used as flavoring for other food products, such as breakfast cereals, hot and cold beverages, and desserts. These products can also be found in gravies, breading, whole, dry or evaporated milk, yogurt, sherbet, breads, waffles, creamed vegetables, mashed potatoes, pudding, creamer or any i diverse products such as hot dogs or spaghetti.
Milk products, which are marketed today as hypo-allergenic milk, are neither uniformly hypoallergenic to all patients, nor made from cow's milk. For example, heat processed milk, in which albumin is denatured, is of modest benefit to only a limited number of patients.
A hypoallergenic vegetable soybean milk formulation devised in China has an objectionable smell and after taste. Hypoallergenic milk produced by the acid process which imitates the stomach's digestive process by utilizing hydrochloric acid to break up proteins, e.g.
casein, has an objectionable smell and taste.
Accordingly, there is a need for a hypoallergenic milk which also has the taste and smell of cow's milk.
U.S. Patent No. 4,293,571 discloses a process for the purification of purified protein hydrolysate. In this process, an aqueous solution of protein is subjected to hydrolysis, then is heat treated to denature the protein. The heat-treated material is then ultrafiltered to eliminate protein.
U.S. Patent No. 4,402,938 discloses a food and method for making the same from colostrum and milk. In this process, the udder of an ungulate is stimulated with an antigen-like material so that the food factor of the whey is enhanced. The enhanced milk is subsequently ultrafiltered. The retentate is discarded and the permeate is saved. Preservatives are added to the milk/colostrum prior to ultrafiltration.
The inventor's cQmmpnly assigned U,S. Patent Nos. 4,954,361 and 5 064,674 , filed August 3, 1990 disclose hypoallergenic milk products from ultrafiltration of cow's milk. The resulting good fasting products are substantially free of cow's milk protein and fat. While these products represent an advance in the state of the art, they are derived from whole milk or fractions thereof . What is needed is a product which has the good taste and nutritional value /lcw _4_ ~~~p~~~iY' of milk-based products, but which may be prepared from synthetic sources. According to the invention hereinafter described, the mineral salts, carbohydrate and protein do not necessarily have to be derived from milk.
summary pf the Inv~nta.~xa A hypoallergenic milk product is provided comprising (i) a mineral salt component comprising a mixture of mineral salts approximating the mineral content of natural milk, (ii) a carbohydrate component comprising one or more carbohydrates and (iii) a hypoallergenic protein component which may advantageously comprise hypo-allergenic protein per se, amino acids, polypeptides having a molecular weight of not more than about 5.0 kDa, or a combination thereof.
The hypoallergenic milk product is prepared by the steps of forming an aqueous mixture of the mineral salt component, carbohydrates, and hypoallergenic protein com-ponent. One or more of the separate components, and/or any combination thereof, are filtered through a filtration membrane, which will only allow molecules with a molecular weight of less than or equal to about 5 kDa to pass therethrough. The permeate is thereafter collected and utilized. The product may be optionally supplemented with hypoallergenic fat, vitamins and addi tional minerals. The filtration steps) are necessary for any components which may be derived from milk sources, e.g., whey-derived components, or components which may otherwise contain allergenic protein.
It is an object of this invention to produce a new and useful hypoallergenic food product from a synthetic or natural mixture of the mineral salts found in natural mammalian milk, and from non-milk or milk-derived car bohydrates or mixtures thereof.
It is another object of this invention to obtain the good taste of natural whole or skim milk.
/lcw It is an object of the invention to provide a hypoallergenic milk product which retains the nutritional content of natural milk.
It is an object of the invention to provide a 5 hypoallergenic milk product which may serve as a vehicle for delivery of specialized nutritional products.
It is an object of the invention to provide a hypoallergenic milk product at low production cost, pos sessing the excellent taste of farm fresh milk, yet free of animal protein.
~etail.ed ~escrxgtion of th~ Tnvention The hypoallergenic milk product disclosed herein after is formulated upon the fact that protein contained in natural milk is the source of allergens that react with reagins to produce allergic reactions. Similar to the antigen-antibody reaction, it is believed that the allergen molecules in cow°s milk, which usually are proteins, have a specific structure which acts as a "key", while the reagins have a corresponding structure which acts as a "lock". While this is the theory upon which the hypoallergenic milk is based, this theory is not meant to be limiting upon the embodiments disclosed hereinafter.
A mixture of mineral salts wYiich will provide the inorganic content of mammalian milk, forms the basis of the present product. The mineral salt mixture, typically in the form of an aqueous solution thereof, is combined with carbohydrate, and a hypoallergenic protein component in amounts approximating the mineral salt, carbohydrate and protean content of natural milk. Hypoallergenic fat is optionally added. Further optional ingredients include additional minerals and trace minerals, vitamins and flavoring agents. Preferably, the hypoallergenic milk product contains, on a weight percentage basis, about 0.7-0.9% mineral salts, about 3 - 4% carbohydrate, about 1-4% hypoallergenic protein component, about 0-4%
/lcw -g_ hypoallergenic fat, the balance comprising water and minor optional ingredients.
The mineral salt mixture may be prepared syntheti cally, by combining mineral salts which will provide a solution approximating the mineral content of natural milk, in the approximate proportions found in milk.
Mineral salts useful for this purpose include the follow-ing: potassium phosphate (monobasic), potassium citrate, sodium citrate, potassium sulfate, calcium chloride, magnesium citrate ~monobasic), potassium carbonate, and potassium chloride. They are combined and dissolved in the appropriate volume of water to form a mineral salt solution which is the basis for the present hypoallergenic synthetic milk product. While the above salts represent the more significant mineral salts of cow's milk, "mineral salt mixture" as used herein shall mean a mixture of substantially the maj ority of the above salts, or equivalent salts providing the mineral content of natural milk, plus any additional organic or inorganic salts which may be added without prejudice to the taste of the resulting product. The mineral salt component thus prepared is of course hypoallergenic since it contains no protein.
Whey is the component of milk which remains after all or a substantial portion of the fat and casein con tained are removed. Various fractions or variants of whey are known, such as, for example, sweet whey permeate (i.e., permeate of whey prepared by crude filtration), dried sweet whey, demineralized whey, delactose whey, whey protein concentrate, and the like. Delactosed whey, that is, whey from which the lactose has been removed, may be advantageously utilized as a natural source of the milk mineral salt component of the present hypoallergenic milk product. Delactosed whey is commercially available in liquid or powder form.
The carbohydrate component may comprise any suitable monosaccharide, disaccharide, polysaccharide or com-/lcw bination thereof. Such sugars include, but are not necessary limited to lactose, galactose, glucose, sucrose, fructose, maltose, maltodextrins and mixtures thereof. The preferred sugar is lactose, which is derived from milk. However, care should be taken in processing carbohydrate from commercial sources, particulary the milk sugars such as lactose, before inclusion in the hypoallergenic milk product. Lactose purified from cow's milk or whey contains as much as 0.01 wt.% milk protein, which becomes trapped in the lactose crystals during the purification process. carbohydrate derived from non-milk sources may also contain small but significant amounts of allergies. For example, sugars derived from corn syrup or sugar cane may contain 0.1%
protein. Thus the carbohydrate component of the invention is advantageously ultrafiltered before use as hereinafter described, or alternatively, the complete milk product as constructed from its component parts is ultrafiltered. Ultrafiltration, as hereinafter described, will remove any trace amounts of hypoaller-genic protein which may be contained in the carbohydrate component.
The hypoallergenic protein component may comprise hypoallergenic protein per se, such as protein from cereal or vegetable sources. Alternatively, or additionally, it may comprise free amino acids, or poly peptides of animal source, provided tre polypeptides are not larger than about 5 kDa, preferably not larger than about 3.5 kDa, more preferably not larger than about 2 kDa, and most preferably not more than about 1 kDa.
Sources of hypoallergenic protein include, but are not limited to: oat cereal (which has a high protein level of about 18%): rice cereal: barley cereal; or any other food source having a low allergenicity and ample protein content. Vegetable sources of protein may also be used, so long as they have a low allergenic potential.
Vegetable sources of low allergenic protein include, for /1Cw _g_ example, potato and soy isolate. Combinations of the foregoing proteins may also be used.
Oat cereal, for example oatmeal, is preferred because it not only enhances the protein content, but also adds to the taste of the resulting product. The oat cereal is used as a very finely ground flour, to facilitate dissolution into the permeate. About 5 to 10 grams of the very finely ground and sieved cereal flour is added to about 100 cc of product. The resulting 20 mixture has a protein content of about 0.9 to 1.8~ by weight, which is similar to human breast milk.
When cereals are used, protein say isolate may else be added to enrich the lysine amino acid value of the cereal. Additionally, the protein may be supplemented with, among other things, methianine, cystine, and iodine to meet the minimum daily requirements.
Protein soy isolate is preferred for use in hypoal-lergenic milk which is intended far infants, who require a single source of protein, or children and adolescents with important growth factor requirements. Cereal hypoallergenic protein sources can be used in the hypoallergenic milk for adults. For example, if a multiple source of protein is desired, any combination of hypoallergenic protein sources may be used.
Tn lieu of, or in addition ta, supplementation with hypoallergenic protein, the product may be supplemented with amino acids, short chain polypeptides, or a combina~
tion of amino acids and short chain polypeptides. By "short chain polypeptide" is meant a polypeptide having a molecular weight of not more than about 5 kDa, preferably not mare than about 1.5 kDa, mare preferably not more than about 1 kDa. Free amino acids and short chain polypeptides are hypoallergenic regardless of source, and therefore will net contribute to the allergenicity of the milk product. Preferably, the amino acids comprise a mixture of amino acids, most preferably a mixture containing at least the nine amino acids which /lcw -are essential to the human diet:
Threonine Valine Phenylalanine Methionine Isoleucine Histidine Lysine Leucine Tryptophan The short chain polypeptides may comprise individual polypeptides or a mixture of polypeptides. The short chain polypeptides and amino acids may be obtained by appropriate hydrolysis of any suitable polypeptides or proteins. Preferably, they are obtained from milk pro-teins, so that the reconstituted hypoallergenic milk product of the invention maintains a portion of the protein nutritional content of whole milk. Hydrolysates of milk proteins are commercially available. For example, a series of hydrolysates are produced by Deltown Chemurgic Corporation, Fraser, New York, under the trademark "DELLAC'°. "DELLAC'° CE80PS is a highly hydrolyzed pancreatic digest of casein. "DELLAC'° LE80PS
is a hydrolyzed pancreatic digest of another milk protein, lactalbumin. High-performance liquid chromatography indicates that these products are free of polypeptides having a molecular weight of greater than about 1.5 kDa. Hydrolysates of non-milk proteins may also be employed, e.g. "DELLAC°' SE50M, which is a papaic digest of soy flour. Other milk protein hydrolysates are sold under the trademark '°ALATAL" by New Zealand Milk Products, Inc., Petaluma, CA (a division of the New Zealand Dairy Board, Wellington, New Zealand).
Each of the aforementioned products may be advantageously utilized in the practice of the invention since they are either free of allergenic milk protein, or are at least free of any milk-derived polypeptides large enough to be considered allergenic.
The protein component is optionally ultrafiltered prior to addition to the other product components . Where the protein component comprises vegetable protein, amino /lcw ~~~~~J~
acids, or short chain polypeptides, filtration is probably not necessary, as these materials are hypoallergenic. However, where filtration of the protein component is practiced, either separately or by filtration of the completed milk product, it may then be feasible to utilize as a source of short chain polypeptides milk protein hydrolysates which may include some polypeptide spacies large enough to be considered hyperallergenic. These larger species will be removed by the filtration step. Preferred such milk protein hydrosylates include °'ALATAL'° 817 and °'DELLAC" LE80GF, which are enzymatic digests of lactalbumin. The latter product consists of 80% by weight protein-derived materials, of which 97 wt.% comprises short chain polypeptide and 3 wt.% whole protein. "DELLAC" LE80GF
has an average molecular weight to about 2 kDa.
Hydrolysates of lactalbumin are particularly prefer-red for supplying short chain polypeptides and/or amino acids in the practice of the present invention. Lactal bumin and its hydrolysates contain a relative surplus of the four essential amino acids lysine, methionine, threonine and isoleucine. They can, therefore, be an important supplement to cereal or vegetable protein which is somewhat deficient in these amino acids. Lactalbumin hydrolysates are particularly useful when combined with other protein sources, such as soy isolate or casein hydrolysate, which may be somewhat deficient in the amino acids cystine and methionine.
It should be noted that while the aforementioned refined polypeptide-containing products result from enzyme hydrolysis of single milk proteins, their addition to the permeate prepared according to the present invention does not significantly impact on the taste of the final reconstituted hypoallergenic milk product.
This should be contrasted with the situation where whole milk, before filtration, is treated in situ with hydrolytic enzymes. This form of in situ hydrolysis of 4403-g CN 2 /lcw milk proteins deleteriously impacts on the taste of the resulting product.
The sources of the optional fat component may include deproteinized clear butter and butter oil or butter fat, polyunsaturated and mono- and/or polyunsaturated vegetable oil or fat from milk free margarine sources, sesame, safflower, and the like, or mixtures thereof. The foregoing fats are hypoallergenic.
The fat is optionally added to the mineral salt solution so that the fat content of the final product ranges between 0% and about 4% by weight depending upon whether skim, 1%, 2% or 4% homogenized milk is desired.
Fox adults where atherosclerosis prevention is of great importance, the fat source may comprise about 1/4 to about 1/2% deproteinized butter oil and/or about 1/2 to about 2% low fat polyunsaturated vegetable fat.
Deproteinized hypoallergenic butter for supplement-ing the permeate may be made from commercially available salt-free, sweet 99.99% anhydrous milk fat. The milk fat is melted in boiling water. The resulting butter oil is then removed from the boiling water, such as by pipetting it off the surface of the water. The boiling water results in extreme heat denaturation of protein and also renders the resulting heat-denatured protein insoluble.
The process removes, by dilution and washing of the milk fat with water, any protein which may be contained in the fat as a contaminant. The process may be repeated any number of times to ensure the purity of the resulting butter product.
Vitamins, and further minerals in addition to those present in the mineral salt component, are also optionally added to the protein- and fat-supplemented mineral salt solution. Such vitamins and minerals are added, so that the resulting milk products meet the minimum daily requirement. By way of non-limiting example, the following may be added, based upon one quart of mineral salt solution supplemented with carbohydrate, /lcw hypoallergenic protein and fat: 400 micrograms of water dispersible Vitamin D; 2100 micrograms of water-dispersible Vitamin A; 60 milligrams of Vitamin C
acetate; folic acid; calcium pantothenate: biotin;
pyridoxine; vitamin B3; vitamin K~ (0.1 mg/1) ; vitamin B12 (1.5 mg/1); vitamin E (20 ~l/1); thiamin (0.6o mg/1);
riboflavin (0.6 mg/ml); vitamin B6 (0.4 mg/ml); minerals such as calcium as phosphate, carbonate or triphosphate;
iron as ferrous sulfate; and zinc as zinc sulfate. Other added minerals may advantageously include cupric sulfate, sodium iodide, potassium carbonate; potassium chloride;
and sodium selenite. Preferred amino acids which may be added include L-cysteine, L-tyrosine and L-tryptophan.
The foregoing are exemplary of the vitamins and minerals that may be added to the hypoallergenic milk. Of course, other vitamins and minerals which are known to those of ordinary skill in the art may also be added.
Additives to enhance the flavor and consistency of the hypoallergenic milk may also be added. Exemplary 2o additives includes hypoallergenic bean gum derived from guar gum (for example, about 3.7 to about 4.7 kg of bean gum per 10,000 liters of hypoallergenic milk);
carrageenan; and/or lecithin of hypoallergenic vegetable bean source, such as soy bean (for example, about 24 kg/10,000 liters of hypoallergenic milk) or say-derived emulsifier. Each of these additives imparts a creamy consistency (acts as an emulsifier) to the hypoallergenic milk. Natural vanilla may also be added to enhance the flavor of hypoallergenic milk.
The hypoallergenic protein component, or mineral salt component when derived from a milk product such as delactose whey, or the carbohydrate component when it comprises lactose or other milk-derived sugar, may be ultrafiltered to ensure that no hypoallergenic protein contaminants are included in the hypoallergenic milk product. The components may be filtered separately or in combination with one another.
/lcw -13- ~~~~~~7~'7 a .fi ~) ~,i Accarding to one embodiment, an ultrafiltration mem-brane utilized for filtration is sized to prevent the passage of any substance with a molecular weight greater than 5 kDa. Such excluded substances include, but are not limited to: milk protein; viable or non-viable bacteria; bacterial protein antigen; and milk fat.
Alternately, ultrafiltration membranes which prevent the passage of any substance with a molecular weight greater than 1 or 2 kDa may also be used. Ultrafiltration mem-branes capable of preventing the passage of 1 or 2 kDa molecular weight substances have proportionately smaller pore sizes.
The following contaminating milk proteins which may be present in protein or carbohydrate components derived from milk sources, are trapped by the ultrafiltration membrane (molecular weights are noted in parenthesis)a alpha lactalbumin (14 kDa); kappa casein (23 kDa); alpha S-1 casein; alpha S-2 casein; beta casein (24 kDa); beta lactoglobulin (37 kDa); bovine serum albumin (f>5 kDa);
and immunoglobulins (>100 kDa). These milk proteins are considered allergenic. Beta lactoglobulin is a dimer at pH 6.6.
It has been found that decreasing the sizing of the filter decreases the relative amount of three milk proteins - alpha lactalbumin, beta lactoglobulin and bovine serum albumin. Thus, where 0.27, 0.33 and 0.01 units of these proteins, respectively, are found in products prepared with a 10 kDa membrane (i.e., a filter membrane which excludes molecules having a molecular weight greater than 10 kDa) , products prepared with 5 kDa dalton filters contain 0.03, 0.03 and 0.01 units of these same proteins, respectively. A dialysate product, prepared using a 3.5 kDa dialysis membrane, contains less than 0.01 units of each of these protein species, resulting in a protein-free dialysate, based upon the limits of the electrophoretic method employed to analyze for protein.
/lcw Ultrafiltration membranes having a 3.5 kDa or less molecular weight cut-aff are preferred. Polyether sulfone membranes having a 1 kDa or 2 kDa cut-off are available, for example, from Advanced Membrane Technology, San Diego, CA and Dow Denmark, Naskov, Denmark, respectively. Ultrafiltration membranes made of ceramic materials may also be used.
Ceramic filters have an advantage over synthetic filters. Ceramic filters can be sterilized with live steam so that the chemical agents, such as chlorine, do not have to be used to sterilize the filter. Synthetic filters, on the other hand, cannot be sterilized with live steam, but instead they must be sterilized with chemical agents, for example, a solution of 200 parts per million (p.p.m) chlorine solution may be used to disinfect the membrane. If a chemical agent is used to disinfect the membrane, the chemical agent may be washed from the filter by flushing the filter with two passes of milk.
A pressure gradient is preferably applied across the ultrafiltration membrane to facilitate filtration.
Preferably, the pressure gradient is adjusted to maintain a filter flux of about 24 liters/m~-hour, which is the typical dairy plant filter flux. The filter is advan-tageously first primed with a small amount of product and the permeate discarded, prior to beginning filtration.
Priming of the filter in this manner is believed to be advantageous to filtering efficiency.
The pH of the product during filtration should be within the range of about 2 to about 11. The preferred pH is about 6.6.
The temperature of the product component during ultrafiltration should be within the range of about 4°C
(i.e., about 40°F) to about 66°C (i.e., about 150°F).
Instead of ultrafiltration, any allergenic com-ponents of the milk component may be removed by dialysis.
As is well known, dialysis operates on a principal akin /lcw 1~
to osmosis. Any allergenic protein in the permeate is effectively trapped utilizing a 5 kDa, (preferably a 3.5 kDa, 2 kDa or 1 kDa) ultrafilter or dialysis membrane.
With dialysis, as with ultrafiltration, the permeate that passes through the membrane, i.e. the hypoallergenic component, is saved and utilized. The retentate, i.e., the material which does not pass through the membrane, is discarded or utilized in other commercial applica tions. A dialysis membrane capable of preventing the passage of materials with a molecular weight of 5 kDa may be used. Other membranes, however, could be used so long as the hyperallergenic component is excluded from the permeate.
Preservatives such as phenol, parabens etc. are preferably not added to the hypoallergenic milk product.
The product may be supplemented, as discussed above, while in liquid form. Alternatively, or additionally, it may be freeze dried in any conventional manner, then reconstituted with liquid supplements at a later time.
After the hypoallergenic protein component, car-bohydrate and optional fats, vitamins and additives to enhance flavor and consistency have been added to the mineral salt solution, the resulting hypoallergenic milk is preferably blended in an emulsifying and diffusing ap-paratus operating at between about 2,500 and about 3,500 r.p.m., to ensure thorough mixing. The blended hypoallergenic milk is then homogenized at a pressure ranging from about 138 to about 276 Bar (i.e., about 2,000 to about 4,000 P.S.T.), pasteurized at about 77°C
(170°F) for about 30 minutes, and then flashed sterilized at about 143°C (290°F) for about 12 seconds and packaged into a aseptic containers. Such containers are made of materials which will not leach into the packaged product.
The materials include, but are not limited to, glass, waxed cardboard or metal. Alternatively, the product may be pasteu~.rized before the various supplements have been added.
4403°8 C3~1 2 /lcw The meticulous removal of substantially all aller-genic protein by an ultrafilter or dialysis membrane has superior advantage regarding hypoallergenicity. The product is free of protein as evidenced by the absence of protein bands upon SDS-PAGE, sensitive to the application of 30 nanograms/microliter or greater concentration of protein. Thus, it is understood 'that as used herein, the expression "substantially completely deproteinized°' or "substantially free of milk protein°' in referring to a preparation, means a preparation free of protein bands upon SDS-PAGE sensitive to the presence of protein concentrations of 30 nanograms/microliter or higher.
Where the carbohydrate component comprises lactose, lactase enzyme may be added to the hypoallergenic milk for use by lactose--intolerant individuals. Alterna tively, in lieu of the preferred sugar lactose, other sugars may be utilized, such as glucose, fructose, maltose or maltodextrin.
The hypoallergenic milk may be substituted for milk components in any formulation in which milk components are used. For example, hypoallergenic milk may be used as a beverage or in beverages, or solid food products such as candy, milk chocolate, cookies, cakes, breakfast cereals and the like. The hypoallergenic milk product may also be utilized as a vehicle for the delivery of specialized nutritional products, which might otherwise have an objectionable taste to the patient. Thus, use of the hypoallergenic milk product as a vehicle for offensive-tasting enteral products may obviate the need for introducing such products by stomach tube, which occurs in patients suffering from such diseases as ileitis, colitis, and geriatric nursing home patients.
One non-limiting hypoallergenic milk product according to the present invention suitable for infants contains the following components, based upon 100 ml of product. The amount of each component may be adjusted _17_ rr s.
'~ l~ ~) according to need.
Protein Soy protein isolate 1.8 g Oatmeal protein (optional) 0.9 to 1.8 g ~'at 3.7 g Oarbotaydrate Lactose (or equivalent 4.6 g concentration of sucrose, maltose, glucose or fructose) Minerals Sodium 41 to 49 mg Potassium 140 to 152 mg Calcium 110 to 119 mg Phosphorus 89 to 93 mg Chloride 63 to 65 mg Iron (fortified) 0.05 to 1.2 mg Zinc 0.38 to 0.43 mg Iodine 10 micrograms Amino ~9.cids Methionine 10 micrograms Cystine 10 micrograms Vitamins Vitamin A 210 International Units (water dispersible) ("I.U.") Vitamin C 6.0 mg (as acetate) Vitamin D 42 I.U»
(water dispersible) Vitamin E 1.0 mg Thiamine 0.04 mg Riboflavin 0.14 to 0.16 mg Niacin 0.08 mg Pyridoxine 0.04 to 0.05 mg Vitamin B~2 0.32 micrograms Folic Acid 5.0 micrograms According to another embodiment, a hypoallergenic milk product suitable for infants has the foregoing com-ponents, except that the soy protein isolate, methione and cystine are omitted in favor of a mixture of free amino acids, comprising preferably at least all the essential amino acids, or, alternatively a mixture of short chain polypeptides. Preferably, the infant formula utilizes a mixture of short chain polypeptides derived from milk protein, such as any of the available hydrolysates of milk proteins described above.
The method of the invention is effective in prepar-ing a hypoallergenic milk product wherein the hypoaller-genic protein content of milk is reduced from 3.6o to 0.26, a reduction of more than 90%, and lower, by utilizing a filter capable of retaining 5 kDa molecular weight, more preferably filters capable of retaining even smaller molecules.
The invention will now be described in more detail with reference to the following specific, non-limiting examples:
/lcw -19- ~~r~r~~~
To 10 liters of distilled water, 800 grams of "AhATAL°' 817 milk protein hydrosylate (New Zealand Milk Products) were added, as well as 150 grams of lactose obtained by crystallization of a whey permeate, and 1400 ml of a mixture of milk mineral salts in the form of a delactosed whey permeate. This mixture was purified and deproteinized by passage through a 1 kDa cut-off ultrafiltration membrane ("A.E.S.-1", Advanced Membrane Technology, San Diego, CA) at about 21°C (i.e., about 70°F). Filtration was facilitated by maintaining a pressure gradient of about 15 Bar (i.e., about 211.5 p.s.i.) to provide a filter flux of 24 liters/m2-hour or more. The final ultrafiltered permeate was then trans ferred to screw top containers.
Example 1 was repeated substituting the 2 kDa cut off ultrafiltration membrane ("GR90 2K°°, Dow Denmark, Naskov, Denmark) for the 1 kDa cut-off ultrafiltration used in Example 1. The pressure gradient required was increased to about 15 Bar (i.e., about 211.5 p.s.i.).
The permeate was then transferred to screw top containers.
500 ml of the final ultrafiltered permeate of Example 1 was pasteurized at about 72°C (i.e., about 162°F) for 20 minutes. This heating further serves to denature any remaining trace of protein. The permeate was then supplemented by adding 2.5 ml of cleared butter oil (as prepared according to Example 5 of U.S. Patent 4,954,361). The supplemented permeate was then homogen-ized using a homogenizes operating at 9000 r.p.m. The formulation was then decanted into two 4 ounce glass bottles and refrigerated.
/lcw '20 ~~ ~ ~~~~r EX~MPIaE 9~
500 ml of the final ultrafiltered permeate of Example 2 was pasteurized at about 72°C for 20 minutes.
This heating further serves to denature any remaining traces of protein. The permeate was then supplemented with 2.5 ml of cleared anhydrous butter oil.
EX~1RSPLE 5 To 10 liters of distilled water was added 800 grams of "ALATAL" 817 milk protein hydrosylate. This was deproteinated by passage through a 1 kDa cut-off ultrafiltration membrane ("A.E.S.-1", Advanced Membrane Technology, San Diego, CA) at about 27.°C (i.e., about 70°F). Filtration was facilitated by maintaining a pressure gradient of about 5 Bar (i.e., about 75 p.s.i.) to provide a filter flux of 24 liters/m2-hour or more.
500 ml of the ultrafiltered permeate was then transferred to screw top containers. Twenty grams of sucrose and 3.79 g of a mineral salt solution were further added.
The final p.H. was 6.7. The mineral salt solution is prepared by grinding and mixing the following quantities of mineral salts in grams:
potassium citrate 5 potassium phosphate (monobasic) 15.80 sodium citrate 21.20 potassium sulfate 1.80 calcium chloride 13.20 magnesium citrate (monobasic) 5.02 potassium carbonate 3.00 potassium chloride 10.78 The above mineral salt quantities are sufficient for 20 liters of stock mineral salt solution. For one later of solution, 7.59 grams of the dry blend are weighed out and dissolved in 975-990 ml of distilled water. Tt was not necessary to adjust pH with 1.0-1.5 N KOH or with 4403-8 CId 2 /lcw °21 magnesium oxide.
EXAMPLE S
Example 5 was repeated substituting the 2 kDa cut°
off ultrafiltration membrane ("GR90 2K", Dow Denmark, Naskov, Denmark) for the 1 kDa cut°off ultrafiltration used in Example 16. The pxessure gradient required was about 15 Bar (i.e., about 211.5 p.s.i.). Seven grams of refined powdered dextrose was added to 200 ml of Example 6 instead of sucrose as in Example 5. The permeate was then transferred to screw top containers.
Ex~~LE a 500 ml of the final ultxafiltered and enriched permeate of Example 5 was pasteurized at about 72°C for minutes. The permeate was supplemented with 2.5 ml of cleared butter oil. The enriched permeate was then homogenized using a homogenizer.
500 ml of the final ultrafiltered permeate of Example 6 was pasteurized at about 172°C, supplemented with 2.5 ml of cleared anhydrous butter oil and homogenized.
EXAMPLE ~
To 10 liters of distilled water were added 800 grams of "ALATAL" 817 and 150 g of lactose prepared by crystallization from whey permeate. The mixture was deproteinized and purified by passage through a lkDa cut-off ultrafiltration membrane ('°A.P.S.-1'° Advanced Membrane Technology, San Diego, CA) at about 21°C (about 70°F). Filtration was facilitated by maintaining a pressure gradient of about 15 Bar (about 211.5 PSI) to provide a filter flux of 24 liters/m~-hr or more. 500 ml of the final ultrafiltered permeate was then transferred to screw-top containers. To an 0.5 later /lcw --22-container of the permeate was added 3.79 grams of the mineral salt dry blend of Example 5 and 20.0 grams of glucose in the form of refined corn syrup. 'rhe final formulation contains about 3.5% carbohydrate (lactose +
glucose); a greatly reduced lactose concentration, without necessitating the use of lactase enzyme.
All steps of Example 9 were repeated substituting the 2 kDa cut-off ultrafiltration membrane ("GR90 2K" Dow Denmark Naskov, Denmark) for the 2 kDa cut-off ultrafiltration membrane used in Example 9. The pressure gradient was increased to about 15 Bar (about 211.5 PSI).
The permeate was then transferred to screw-top containers.
EXAP'tPL~ 3.3.
500 ml of the final ultrafiltered permeate of Example 9 was pasteurized at about 72°C for 20 minutes.
The permeate was then supplemented with 2.5 ml of cleared anhydrous butter oil and homogenized as before.
El~lAP~ipLE 9.2 500 ml of the final ultrafiltered permeate of Example 10 was pasteurized at about 72°C, supplemented with 2.5 ml of cleared anhydrous butter oil and homogenized.
In each of Examples 1-12, about 2.0 grams of a commercial, vanilla-flavored oat soy preparation was added to a 100 m1 sample of the resulting permeate for body, flavor and emulsification enhancement. This mixture was then further homogenized and emulsified with a homogenizer/emulsifier operating at 9,000 r.p.m.
4403°8 CN 2 ~lCw --23- ~~~~~8~
ES~PI~LE ~.3 To 10 liters of distilled water was added 800 grams of "ALATAL°' 817 and 1400 ml of delactose whey permeate.
The mixture was then deproteinized by passage through a 3 kDa cut-off ultrafiltration membrane ("A.E.S.-1") at about 21°C. Filtration was facilitated by maintaining a pressure gradient of about 15 Dar (211.5 PSI) to provide a filter flux of 24 liters/m2--hour or more. The final ultrafilter permeate was then transferred to screw top containers. 0.5 liter of the synthetic permeate is then supplemented with 5 g of dextrose. The final formulation contains about 3.4o carbohydrate (lactose -~
glucose); a greatly reduced lactose without necessitating the use of lactase enzyme.
EXAP2~LE 1.~
Example 13 was repeated substituting the 2 kDa cut off ultrafiltration membrane ('°GR 90 2K") for the 1 kDa cut-off ultrafiltration filter used in Example 13. The pressure gradient was increased to 15 Bar.
500 ml of the final ultrafiltered permeate of Example 14 is pasteurized at about 72°C for twenty minu tes, supplemented with 2.5 ml of cleared anhydrous butter oil and homogenized as before.
EXAMPLE 7.6 500 ml of the final ultrafiltered permeate of Example 15 is pasteurized, supplemented with 2.5 ml of cleared anhydrous butter oil and homogenized.
In each of Examples 13 - 16, a 100 ml sample of the resulting permeate is mixed with about 2.0 grams of a commercial, vanilla-flavored oat soy preparation as set out above for body, flavor and emulsificatian enhance-/lcw ment.
The presence of medication utilized to treat milk-producing cows is undesirable in milk for human consump-tion. The ultrafiltration method described herein is believed to effectively reduce the level of veterinary pharmaceuticals contained in fractions of cow°s milk.
Approximately 750 of monocyclic drugs, e.g., penicillin and sulfonamides, which may be present in the milk, are attached to the milk's protein fraction. Approximately 25~ or more of tricyclic compounds, and approximately 50~
of bicyclic compounds, are similarly found attached to the protein fraction. Thus, it may be readily appreciated that removal of milk protein, as in the practice of the present invention, serves also to substantially reduce the level of veterinary medications which may be contained in cow's milk.
Recently, bovine immunodeficiency infection in cows has been reported to result in decreased milk production.
While this viral agent has not been found to be transmis-sible to humans, the ultrafiltration procedure utilized herein excludes viruses and bacteria, which are generally larger than 100 kLla and 1,000 kDa respectively. Thus, ultrafiltration ensures that these disease agents which may be carried in milk protein hydrosylates and/or delactose whey permeates utilized in the practice of the invention, do not infect the resulting hypoallergenic milk product.
The anaphylactic type of allergic reactions may be caused by milk proteins. Many patients, particularly children, have symptoms of recurrent colds, bronchitis, asthmatic bronchitis, asthma, as well as recurrent sinusitis and/or otitis. These symptoms are often relieved by avoidance of milk. These symptoms may actually be caused by viral or bacterial protein present in milk. Where milk components such as milk protein hydrosylates and delactose whey are utilized in the practice of the present invention, the ultrafiltration /lcw -?5-procedure removes protein, as well as bacteria and viruses which may cause these symptoms.
'fhe present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof and, accordingly, reference should be made to the appended claims, rather than to the foregoing specification, as indicating the scope of the invention.
Claims (27)
1. A palatable hypoallergenic product characterized by including the following components:
(a) a mineral salt component substantially free of allergenic protein, the mineral salt component includes a mixture of mineral salts having approximately the mineral content of natural milk;
(b) a carbohydrate component substantially free of allergenic protein, the carbohydrate component including one or more carbohydrates; and (c) a protein component substantially free of allergenic protein, the protein component is selected from the group consisting of hypoallegenic protein, amino acids, polypeptides and combinations thereof.
(a) a mineral salt component substantially free of allergenic protein, the mineral salt component includes a mixture of mineral salts having approximately the mineral content of natural milk;
(b) a carbohydrate component substantially free of allergenic protein, the carbohydrate component including one or more carbohydrates; and (c) a protein component substantially free of allergenic protein, the protein component is selected from the group consisting of hypoallegenic protein, amino acids, polypeptides and combinations thereof.
2. The product according to claim 2, wherein the mixture of mineral salts is derived from delactosed whey.
3. The product according to claim 1 or 2, wherein the protein component is derived from milk protein, cereal protein, vegetable protein, casein, soy flour or lactalbumin, or any combination thereof.
4. The product according to any one of claims 1, 2 or 3, wherein the carbohydrate component is derived from lactose or deproteinized lactose or any combination thereof.
5. The product according to any one of claims 1, 2, 3 or 4, wherein the product is further characterized by including hypoallergenic fat, deproteinized butter or vegetable oil, or any combination thereof.
6. The product according to any one of claims 1, 2, 3, 4 or 5, wherein the product is characterized by further including hypoallergenic milk chocolate prepared with hypoallergenic milk.
7. The product according to any one of claims 1, 2, 3, 4, 5 or 6, wherein the protein component has a molecular weight of not more than 5 kDa.
8. The product according to any one of claims 1, 2, 3, 4, 5, 6 or 7, wherein the protein component has a molecular weight of not more than 3.5 kDa.
9. The product according to claim 8, wherein the protein component has a molecular weight of not more than 2 kDa.
10. The product according to claim 9, wherein the protein component has a molecular weight of not more than 1 kDa.
11. The product according to any one of claims 8, 9 or 10, wherein the product is substantially free of protein of animal, vegetable or cereal grain origin as determined by the substantial absence of protein bands upon sodium dodecyl sulfate polyacrylamide gel electrophoresis and silver staining.
12. A process for making a palatable hypoallergenic product characterized by preparing a mixture which includes:
(a) selecting a hypoallergenic protein component which is substantially free of allergenic protein, the hypoallergenic protein component is selected from the group consisting of a hypoallergenic protein, amino acids or polypeptides, or any combination thereof;
(b) combining said hypo allergenic protein component with a mineral salt component which is substantially free of allergenic protein and a carbohydrate component which is substantially free of allergenic protein.
(a) selecting a hypoallergenic protein component which is substantially free of allergenic protein, the hypoallergenic protein component is selected from the group consisting of a hypoallergenic protein, amino acids or polypeptides, or any combination thereof;
(b) combining said hypo allergenic protein component with a mineral salt component which is substantially free of allergenic protein and a carbohydrate component which is substantially free of allergenic protein.
13. The process according to claim 12 , wherein the hypoallergenic protein component is prepared by filtering through a filter which will only allow molecules with a molecular weight less than or equal to about 5 kDa to pass therethrough, a protein component selected from the group consisting of a hypoallergenic protein, amino acids or polypeptides, or any combination thereof.
14. The process according to claim 12 or 13, wherein the mineral salt component is prepared by filtering through a filter which will only allow molecules with a molecular weight less than or equal to about 5 kDa to pass therethrough, a mineral salt component.
15. The process according to any one of claims 12, 13 or 14, wherein the carbohydrate component is prepared by filtering through a filter which will only allow molecules with a molecular weight less than or equal to about 5 kDa to pass therethrough, a carbohydrate component.
16. The process according to any one of claims 13, 14 or 15, wherein the filter only allows molecules with a molecular weight less than or equal to about 3.5 kDa to pass therethrough.
17. The process according to claim 16, wherein the filter only allows molecules with a molecular weight less than or equal to about 2 kDa to pass therethrough.
18. The process according to claim 17, wherein the filter only allows molecules with a molecular weight less than or equal to about 1 kDa to pass therethrough.
19. The process according to any one of claims 16, 17 or 18, wherein the hypoallergenic product is substantially free of protein of animal, vegetable or cereal grain origin as determined by the substantial absence of protein bands upon sodium dodecyl sulfate polyacrylamide gel electrophoresis and silver staining.
20. The process according to any one of claims 12, 13, 14, 15, 16, 17, 18 or 19, wherein the mixture of mineral salts is derived from delactosed whey.
21. The process according to any one of claims 12, 13, 14, 15, 16, 17, 18, 19 or 20, wherein the hypoallergenic protein component is derived from milk protein, cereal protein;
vegetable protein, casein, soy flour or lactalbumin, or any combination thereof.
vegetable protein, casein, soy flour or lactalbumin, or any combination thereof.
22. The process according to any one of claims 12, 13, 14, 15, 16, 17, 18, 19, 20 or 21, wherein the carbohydrate component is derived from lactose or deproteinized lactose or any combination thereof.
23. The process according to any one of claims 12, 13, 14, 15, 16, 17, 18, 19, 20, 21 or 22, wherein the process is characterized by preparing a mixture which further includes a hypoallergenic fat, deproteinized butter or vegetable oil, or any combination thereof.
24. The process according to any one of claims 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22 or 23, wherein the process is characterized by preparing a mixture which further includes a hypoallergenic milk chocolate prepared with hypoallergenic product.
25. The product according to any one of claims 1 to 11, wherein the product is used as an alternative to milk.
26. The process according to claim 12, wherein the product is characterized by preparing a mixture which further includes combining with the mixture a fat component.
27. The process according to claim 26, wherein the fat component is provided by adding milk selected from the group consisting of skim, 1%, 2%, and homogenized milk.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US754,872 | 1991-09-04 | ||
| US07/754,872 US5204134A (en) | 1989-01-13 | 1991-09-04 | Hypoallergenic milk products from natural and/or synthetic components and process of making |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2077482A1 CA2077482A1 (en) | 1993-03-05 |
| CA2077482C true CA2077482C (en) | 2003-04-15 |
Family
ID=25036742
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002077482A Expired - Fee Related CA2077482C (en) | 1991-09-04 | 1992-09-03 | Hypoallergenic products from natural and/or synthetic components and process of making |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US5204134A (en) |
| EP (1) | EP0531142B1 (en) |
| AT (1) | ATE149797T1 (en) |
| CA (1) | CA2077482C (en) |
| DE (1) | DE69218073D1 (en) |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1995003706A1 (en) * | 1993-08-03 | 1995-02-09 | Immunopath Profile, Inc. | Dairy permeate-based beverage and process of making |
| AU7553694A (en) * | 1993-08-03 | 1995-02-28 | Immunopath Profile, Inc. | Product and process of making hypoallergenic chocolate compositions |
| US6197356B1 (en) * | 1993-08-03 | 2001-03-06 | Immunopath Profile, Inc. | Process for preparing hypoallergenic foods |
| US5550106A (en) * | 1994-03-04 | 1996-08-27 | Bristol-Myers Squibb Company | Low buffer nutritional composition |
| US5912032A (en) * | 1995-05-11 | 1999-06-15 | Meiji Milk Products Company, Limited | Process of producing calcium-supplemented milk drinks |
| JPH09255526A (en) * | 1996-03-27 | 1997-09-30 | Shiseido Co Ltd | Cosmetic for preventing aging |
| FR2760756B1 (en) * | 1997-03-17 | 2003-09-19 | Richard De Nyons | PROCESS FOR PRODUCING HYPOALLERGENIC VEGETABLE OILS |
| US6403142B1 (en) | 1998-12-11 | 2002-06-11 | Ralston Purina Company | Hypoallergenic pet food |
| US6287607B2 (en) | 1999-07-19 | 2001-09-11 | Mission Pharmacal Company | Potassium calcium citrate compositions and methods therefor |
| NL1016783C2 (en) * | 2000-12-04 | 2002-06-05 | Business Quarters B V | Continuous production of a tryptophan-enriched milk beverage comprises adding a homogenized concentrated mixture of L-tryptophan and water to a milk beverage |
| US20020172766A1 (en) * | 2001-03-17 | 2002-11-21 | Laxman Ravi K. | Low dielectric constant thin films and chemical vapor deposition method of making same |
| US7112424B2 (en) * | 2001-03-19 | 2006-09-26 | Council Of Scientific And Industrial Research | Process for the preparation of protein hydrolysate from legumes |
| BR0210964A (en) | 2001-07-12 | 2004-10-13 | Advanced Food Technologies Inc | Type of cooked food product, method of preparing a cooked convenience food / light meal, product, and method of preparing a food product |
| US20030064154A1 (en) * | 2001-08-06 | 2003-04-03 | Laxman Ravi K. | Low-K dielectric thin films and chemical vapor deposition method of making same |
| US7423166B2 (en) * | 2001-12-13 | 2008-09-09 | Advanced Technology Materials, Inc. | Stabilized cyclosiloxanes for use as CVD precursors for low-dielectric constant thin films |
| US7108771B2 (en) | 2001-12-13 | 2006-09-19 | Advanced Technology Materials, Inc. | Method for removal of impurities in cyclic siloxanes useful as precursors for low dielectric constant thin films |
| US7261911B2 (en) * | 2002-12-19 | 2007-08-28 | Luebbers Steven T | Aseptically packaged, extensively hydrolyzed, liquid nutritional formula and method for making it |
| US7838042B2 (en) * | 2004-04-21 | 2010-11-23 | Albion International, Inc. | Hypoallergenic metal amino acid chelates and metal amino acid chelate-containing compositions |
| US7309506B2 (en) * | 2004-07-15 | 2007-12-18 | Luz Maria Amador | Enzymatic method of producing a lactose-free calcium product |
| KR100509681B1 (en) * | 2005-05-27 | 2005-08-23 | 주식회사 렉스진바이오텍 | Food composition for stimulating growth comprising fraction isolated from mammalian colostrum or milk whey |
| US20060286208A1 (en) * | 2005-06-01 | 2006-12-21 | Nagendra Rangavajla | Methods for producing protein partial hydrolysates and infant formulas containing the same |
| US7618669B2 (en) * | 2005-06-01 | 2009-11-17 | Mead Johnson Nutrition Company | Low-lactose partially hydrolyzed infant formula |
| IL174477A (en) * | 2006-03-22 | 2011-03-31 | Gadot Biochemical Ind Ltd | Calcium enrichment compositions and methods of production thereof |
| DE102012112301A1 (en) * | 2012-12-14 | 2014-06-18 | Jürgen Hower | cocoa food composition |
| CN111248281A (en) * | 2018-11-30 | 2020-06-09 | 内蒙古伊利实业集团股份有限公司 | Method for adding milk mineral salt into yoghourt, jam containing milk mineral salt and yoghourt |
Family Cites Families (42)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US677159A (en) * | 1900-01-06 | 1901-06-25 | John Meyenberg | Process of preparing foods. |
| US1210918A (en) * | 1915-03-22 | 1917-01-02 | Harry B Eigelberner | Process of renovating butter. |
| US2414837A (en) * | 1943-11-17 | 1947-01-28 | Kraft Foods Co | Manufacture of cream products |
| US2437080A (en) * | 1944-03-10 | 1948-03-02 | Sun Chemical Corp | Fractionation of lacteal liquids |
| US2714068A (en) * | 1952-09-25 | 1955-07-26 | American Home Prod | Diacasein and process for its manufacture |
| US2903358A (en) * | 1956-11-15 | 1959-09-08 | Borden Co | Modified milk |
| US3003882A (en) * | 1959-08-25 | 1961-10-10 | Frozen Dessert Company | Imitation ice cream containing highly unsaturated vegetable oil |
| US3642493A (en) * | 1967-06-01 | 1972-02-15 | Ralston Purina Co | Method of preparing a simulated milk product |
| US3669678A (en) * | 1970-11-16 | 1972-06-13 | John Kraft Sesame Corp | Food composition prepared from whey and comminuted sesame |
| US3930039A (en) * | 1971-07-30 | 1975-12-30 | Molkerei J A Meggle Milchindus | Method of preparing a protein concentrate from whey |
| US3978234A (en) * | 1972-05-22 | 1976-08-31 | Societe D'assistance Technique Pour Produits Nestle S.A. | Protein composition |
| US3843838A (en) * | 1973-03-16 | 1974-10-22 | Warwick Electronics Inc | Muting circuit for video and audio playback system |
| FR2239208B1 (en) * | 1973-07-31 | 1977-10-07 | Nestle Sa | |
| FR2288473A1 (en) * | 1974-10-22 | 1976-05-21 | Manche Union Coop Agr Laiti | PROCESS FOR TREATMENT OF CHEESE WHEY, IN PARTICULAR WITH A VIEW OF THE EXTRACTION OF GLYCOPROTEIDES AND SIALIC ACID |
| US4202909A (en) * | 1976-11-22 | 1980-05-13 | Patent Technology, Inc. | Treatment of whey |
| FR2386264A1 (en) * | 1977-04-07 | 1978-11-03 | Legrand Charles | Mfg. milk food for babies and special diets - with main ingredient contg. soluble phase of goat milk including bifidogens |
| US4358464A (en) * | 1977-08-02 | 1982-11-09 | Superior Dairy, Inc. | Process for converting sour whey into sweet whey and product |
| US4341801A (en) * | 1977-10-17 | 1982-07-27 | Dorr-Oliver Incorporated | Ultrafiltration process for the preparation of cream cheese |
| WO1980000399A1 (en) * | 1978-08-25 | 1980-03-20 | Unilever Nv | Milk substitutes |
| CH636248A5 (en) * | 1979-03-09 | 1983-05-31 | Nestle Sa | PROCESS FOR THE PREPARATION OF A PURIFIED PROTEIN HYDROLYSATE. |
| FR2459619B1 (en) * | 1979-06-26 | 1983-07-29 | Agronomique Inst Nat Rech | PROCESS FOR OBTAINING FROM LACTOSERUM, A PRODUCT ENRICHED IN ALPHA-LACTALBUMIN AND APPLICATIONS OF SAID PROCESS |
| FR2465421A1 (en) * | 1979-09-19 | 1981-03-27 | Bel Fromageries | NEW DAIRY PRODUCTS FOR THE MANUFACTURE OF CHOCOLATE PRODUCTS AND PROCESS FOR PRODUCING THE SAME |
| FR2474829B1 (en) * | 1980-02-01 | 1983-09-09 | Agronomique Inst Nat Rech | PROCESS FOR THE TREATMENT OF A CASEIN MATERIAL CONTAINING PHOSPHOCASEINATES OF MONOVALENT CATIONS OR DERIVATIVES THEREOF, PRODUCTS OBTAINED AND APPLICATIONS |
| FI68502C (en) * | 1980-04-29 | 1985-10-10 | Entremont Sa | REQUIREMENTS FOR CONTAINING CONTAINER SEPARATION WITH SMOERFETT |
| US4402938A (en) * | 1980-05-29 | 1983-09-06 | Impro Products, Inc. | Food and the method of extracting the same from colostrum and milk |
| GB2080664A (en) * | 1980-07-29 | 1982-02-10 | Smith Walton J | Potassium supplement composition comprising foodstuffs |
| DE3168085D1 (en) * | 1980-11-22 | 1985-02-14 | Unilever Nv | Water-in-oil emulsion spread having a fat content ranging from 25 to 65 wt%, which comprises a dispersed aqueous phase containing a gelling system |
| ZA822954B (en) * | 1981-05-12 | 1983-04-27 | Lavery D & Son Ltd | Method for the manufacture of cheese with a substantially reduced fat content |
| US4389425A (en) * | 1981-06-11 | 1983-06-21 | Burr Ii Jack | Method of making soy milk containing stabilized protein |
| US4528204A (en) * | 1981-08-27 | 1985-07-09 | Dynagel, Incorporated | Preparation of hydrolyzed collagen-containing products from non-gelled liquid hydrolyzed collagen concentrate and gelled products prepared therefrom |
| NZ202514A (en) * | 1981-11-24 | 1985-08-16 | J Czulak | Whey protein recovery process |
| US4716120A (en) * | 1983-03-17 | 1987-12-29 | Minnesota Mining And Manufacturing Company | Stable allergenic extracts and methods |
| US4614653A (en) * | 1983-07-26 | 1986-09-30 | Cargill, Incorporated | Milk replacer and method of feeding |
| US4547386A (en) * | 1984-02-27 | 1985-10-15 | Purdue Research Foundation | Lactose/cheese whey/whey filtrate semi-solid animal feed supplement |
| US4670268A (en) * | 1985-01-29 | 1987-06-02 | Abbott Laboratories | Enteral nutritional hypoallergenic formula |
| ATE65406T1 (en) * | 1985-01-29 | 1991-08-15 | Abbott Lab | ENTERAL HYPOALLERGENIC NUTRITIONAL RECIPE. |
| DE3867308D1 (en) * | 1987-05-20 | 1992-02-13 | Chugai Pharmaceutical Co Ltd | SALT REPLACEMENT AND FOOD CONTAINING THIS. |
| NL8802525A (en) * | 1988-10-13 | 1990-05-01 | Dmv Campina Bv | PROCESS FOR PREPARING HYPOTONE OR ISOTONE DRINKS. |
| US5185166A (en) * | 1988-12-07 | 1993-02-09 | San-Ei Chemical Industries, Ltd. | Process for the production of milk mineral concentrate and drink containing minerals |
| US4954361A (en) * | 1989-01-13 | 1990-09-04 | Immunopath Profile, Inc. | Hypoallergenic milk products and process of making |
| US5064674A (en) * | 1989-01-13 | 1991-11-12 | Immunopath Profile, Inc. | Hypoallergenic milk products and process of making |
| DE3942028A1 (en) * | 1989-12-20 | 1991-06-27 | Kali Chemie Ag | METHOD FOR FRACTIONATING MILK OR DAIRY PRODUCTS |
-
1991
- 1991-09-04 US US07/754,872 patent/US5204134A/en not_active Expired - Lifetime
-
1992
- 1992-09-03 DE DE69218073T patent/DE69218073D1/en not_active Expired - Lifetime
- 1992-09-03 CA CA002077482A patent/CA2077482C/en not_active Expired - Fee Related
- 1992-09-03 AT AT92308019T patent/ATE149797T1/en not_active IP Right Cessation
- 1992-09-03 EP EP92308019A patent/EP0531142B1/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP0531142B1 (en) | 1997-03-12 |
| DE69218073D1 (en) | 1997-04-17 |
| ATE149797T1 (en) | 1997-03-15 |
| US5204134A (en) | 1993-04-20 |
| CA2077482A1 (en) | 1993-03-05 |
| EP0531142A1 (en) | 1993-03-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2077482C (en) | Hypoallergenic products from natural and/or synthetic components and process of making | |
| EP0469206B1 (en) | Hypoallergenic milk products and process of making | |
| US4954361A (en) | Hypoallergenic milk products and process of making | |
| DE60222420T2 (en) | METHOD FOR HYDROLYSIS OF MILK PROTEINS | |
| US6277426B1 (en) | Dairy product and process for making | |
| AU2012216966A1 (en) | Milk-based product and a method for its preparation | |
| US5153019A (en) | Rice bran-honey based beverage product and process for making same | |
| US20010022986A1 (en) | Dairy permeate-based beverage | |
| US20140141127A1 (en) | Beverage compositions comprising soy whey proteins that have been isolated from processing streams | |
| JP5465834B2 (en) | Liver function protectant | |
| CA2873127A1 (en) | Sterilized liquid protein supplement including a solubility enhancing nutritional protein | |
| US5912040A (en) | Process of making a dairy permeate-based beverage | |
| US5186971A (en) | Hypoallergenic milk products and process of making | |
| AU2020216251A1 (en) | Native whey protein for improving intestinal maturation | |
| PT1527690E (en) | Method of deflavoring whey protein | |
| US5112636A (en) | Hypoallergenic butter and process of making | |
| US6251459B1 (en) | Dairy product and method | |
| NL8802525A (en) | PROCESS FOR PREPARING HYPOTONE OR ISOTONE DRINKS. | |
| EP3917547A1 (en) | Native whey protein for treating and/or preventing intestinal infection | |
| EP0915668B1 (en) | Drinkable, acid nutrient composition with long shelf life and health-promoting action | |
| Craig | Dairy Derived Food Ingredients–Functional and Nutritional Considerations | |
| JP2000287657A (en) | Sterilized beverage containing unmodified lactoferrin, and its production | |
| JP3161810B2 (en) | Process for producing dispersible calcium and / or magnesium, calcium-lipoprotein complex and food and beverage using the same | |
| Lessof et al. | Cow’s Milk and Some Alternatives | |
| NZ715061B2 (en) | Composition with improved digestibility of proteins |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |