CA2077788A1 - Reagent compositions and their use in the identification and characterization of reticulocytes in whole blood - Google Patents

Reagent compositions and their use in the identification and characterization of reticulocytes in whole blood

Info

Publication number
CA2077788A1
CA2077788A1 CA2077788A CA2077788A CA2077788A1 CA 2077788 A1 CA2077788 A1 CA 2077788A1 CA 2077788 A CA2077788 A CA 2077788A CA 2077788 A CA2077788 A CA 2077788A CA 2077788 A1 CA2077788 A1 CA 2077788A1
Authority
CA
Canada
Prior art keywords
reticulocytes
cell
erythrocytes
measured
hemoglobin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2077788A
Other languages
French (fr)
Other versions
CA2077788C (en
Inventor
Gregory M. Colella
Daniel Ben-David
Albert Cupo
Sophie S. Fan
Gena Fischer
Grace E. Martin
Leonard Ornstein
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer Corp
Icahn School of Medicine at Mount Sinai
Original Assignee
Mount Sinai School of Medicine
Miles Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mount Sinai School of Medicine, Miles Inc filed Critical Mount Sinai School of Medicine
Publication of CA2077788A1 publication Critical patent/CA2077788A1/en
Application granted granted Critical
Publication of CA2077788C publication Critical patent/CA2077788C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/101666—Particle count or volume standard or control [e.g., platelet count standards, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/107497—Preparation composition [e.g., lysing or precipitation, etc.]

Abstract

Methods for characterizing and distinguishing reticulocytes and mature red blood cells use reagent compositions which include organic cationic dyes for staining the reticulocytes in the blood sample and buffer solutions for maintaining a pH of about 6 to about 9. The dyes may be the blue absorption dyes Oxazine 750 or New Methylene Blue. When a zwitterionic surfactant is included in the reagent composition for isovolumetric sphering of the reticulocytes and erythrocytes, and the reagent composition and whole blood sample mixture is passed through the sensing region of a flow cytometer, the light scattered and absorbed by each cell is measured, the erythrocytes can be distinguished from reticulocytes and the volume, hemoglobin concentration and the hemoglobin content of each reticulocyte or erythrocyte, and the mean cell volume, mean corpuscular hemoglobin concentration, and mean cell hemoglobin of the reticulocytes or erythrocytes are calculated from the measured cell-by-cell volume and hemoglobin concentration. One method also includes adjusting the measured absorption signals for pseudo-absorption.
CA002077788A 1991-12-05 1992-09-09 Reagent compositions and their use in the identification and characterization of reticulocytes in whole blood Expired - Fee Related CA2077788C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US802,593 1991-12-05
US07/802,593 US5350695A (en) 1991-12-05 1991-12-05 Methods for the identification and characterization of reticulocytes in whole blood

Publications (2)

Publication Number Publication Date
CA2077788A1 true CA2077788A1 (en) 1993-06-06
CA2077788C CA2077788C (en) 1998-07-14

Family

ID=25184159

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002077788A Expired - Fee Related CA2077788C (en) 1991-12-05 1992-09-09 Reagent compositions and their use in the identification and characterization of reticulocytes in whole blood

Country Status (12)

Country Link
US (2) US5350695A (en)
EP (1) EP0545314B1 (en)
JP (1) JP2802710B2 (en)
KR (1) KR100276144B1 (en)
AT (1) ATE161952T1 (en)
AU (1) AU2816592A (en)
CA (1) CA2077788C (en)
DE (1) DE69223930T2 (en)
DK (1) DK0545314T3 (en)
ES (1) ES2111599T3 (en)
IL (1) IL103054A (en)
TW (1) TW287232B (en)

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Also Published As

Publication number Publication date
IL103054A (en) 1997-07-13
IL103054A0 (en) 1993-02-21
DE69223930D1 (en) 1998-02-12
ES2111599T3 (en) 1998-03-16
JP2802710B2 (en) 1998-09-24
EP0545314A1 (en) 1993-06-09
AU2816592A (en) 1993-06-10
KR100276144B1 (en) 2000-12-15
US5438003A (en) 1995-08-01
ATE161952T1 (en) 1998-01-15
DK0545314T3 (en) 1998-09-07
US5350695A (en) 1994-09-27
EP0545314B1 (en) 1998-01-07
DE69223930T2 (en) 1998-04-30
TW287232B (en) 1996-10-01
CA2077788C (en) 1998-07-14
JPH06180314A (en) 1994-06-28

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