CA2093020A1 - Nucleoside derivatives - Google Patents
Nucleoside derivativesInfo
- Publication number
- CA2093020A1 CA2093020A1 CA002093020A CA2093020A CA2093020A1 CA 2093020 A1 CA2093020 A1 CA 2093020A1 CA 002093020 A CA002093020 A CA 002093020A CA 2093020 A CA2093020 A CA 2093020A CA 2093020 A1 CA2093020 A1 CA 2093020A1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
- A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
- A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
- A61K31/519—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim ortho- or peri-condensed with heterocyclic rings
- A61K31/52—Purines, e.g. adenine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
- A61P33/10—Anthelmintics
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
- C07D405/02—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings
- C07D405/04—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings directly linked by a ring-member-to-ring-member bond
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D473/00—Heterocyclic compounds containing purine ring systems
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic System
- C07F9/02—Phosphorus compounds
- C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
- C07F9/645—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having two nitrogen atoms as the only ring hetero atoms
- C07F9/6509—Six-membered rings
- C07F9/6512—Six-membered rings having the nitrogen atoms in positions 1 and 3
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic System
- C07F9/02—Phosphorus compounds
- C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
- C07F9/6558—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing at least two different or differently substituted hetero rings neither condensed among themselves nor condensed with a common carbocyclic ring or ring system
- C07F9/65586—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing at least two different or differently substituted hetero rings neither condensed among themselves nor condensed with a common carbocyclic ring or ring system at least one of the hetero rings does not contain nitrogen as ring hetero atom
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic System
- C07F9/02—Phosphorus compounds
- C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
- C07F9/6561—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing systems of two or more relevant hetero rings condensed among themselves or condensed with a common carbocyclic ring or ring system, with or without other non-condensed hetero rings
- C07F9/65616—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing systems of two or more relevant hetero rings condensed among themselves or condensed with a common carbocyclic ring or ring system, with or without other non-condensed hetero rings containing the ring system having three or more than three double bonds between ring members or between ring members and non-ring members, e.g. purine or analogs
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/06—Pyrimidine radicals
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/06—Pyrimidine radicals
- C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/16—Purine radicals
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/16—Purine radicals
- C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
Abstract
Novel antiviral compounds of formulae (1A) and (1B), wherein X
and R1-R4 are as stated in claim 1 in the form of an .alpha.- or .beta.-anomer or a mixture thereof, and pharmaceutically acceptable salts thereof are disclosed, as well as a new method for preparation thereof. Said compounds can be used for treatment of infections caused by viruses requiring reverse transcriptase for replication, such as HIV and hepatitis B virus.
and R1-R4 are as stated in claim 1 in the form of an .alpha.- or .beta.-anomer or a mixture thereof, and pharmaceutically acceptable salts thereof are disclosed, as well as a new method for preparation thereof. Said compounds can be used for treatment of infections caused by viruses requiring reverse transcriptase for replication, such as HIV and hepatitis B virus.
Description
O 92/06102 2 ~ 9 3 0 2 0 P ~ /SE91/00653 ' NUCLEOSIDE DERIVATIVES
Field of the _nvention The present invention relates to novel chemical compounds and pharmaceutically acceptable salts thereof which can be used in therapy for therapeutic and prophylactic treatment of the acquired immuno deficiency syndrome (AIDS) and infections caused by viruses requiring reverse transcriptase for replication, such as human immuno deficiency viruses and hepatitis B virus, and also for treatment of other virus diseases, such as those of herpes vlruses, diseases which include both common in~ections and neoplastic diseases, i.e. cancer.
Backaround of the invention The effects of viruses on bodily functions ls the end result of changes occurring at the cellular and subcellular levels. The pathogenic changes at the cellular level are different for different combinations of viruses and host cells. While some viruses cause a general destruction (killing) o~ certain cells, other may transform cells into a neoplastic state.
Important common viral infections are herpes dermatitis (including herpes labialis)`, herpes keratitis, herpes genitalis, herpes zoster, herpes encephalitis, inEectious mononucleosis and cytomegalovirus infections all of which are caused by viruses belonging to the herpes virus group. Other important viral diseases are influenza A and B which are caused by influenza A
and B virus, respectively. Another important common viral dis-ease is viral hepatitis and especially hepatitis B virus infec-tions are widely spread. Effective and selective antiviral agents are needed for treatment of these diseases as well as for other diseases caused by viruses.
Several different viruses of both DNA and RNA type have been shown to cause tumors in animals. The effect of cancerogenic chemicals can on animals result in activation of latent tumor viruses. It is possible that tumor viruses are involved in human tumors. The most likely human cases known today are leukemias, sarcomas, breast carcinomas, Burkitt lymphomas, nasophopharyngal carcinomas and cervical c~ncers where RNA tumor viruses and herpes viruses are involved. This makes the search for selective : .
. : ' : ' :"" :' ~
WO~2/06102 2 ~ ~ 3 0 ~ ~ PCT/S~91/0~653 -inhibitors of tumorogenic viruses and their functions an important undertaking in the efforts to treat cancer.
In the late seventies a new disease was reported, which subse~uently was refe~red to as Acquired Immuno Deficiency Syndrome (AIDS). It is now generally accepted that a retrovirus referred to as HIV (Human Immunodeficiency Virus), formerly known as Human T-cell Lymphotrophic Virus (HTLV-III) or Lymphadenopathy Associated Virus (LAV) plays an essential role in the etiology of AIDS. Different types of HIV have been found, such as HIV-l and HIV-2 and more are likely to be isolated.
AIDS is characterized by a profound immunodeficiency due to low numbers of a subset of lymphocyte-T-helper cells, which are one target for HIV infection. The profound immunodeficiency in AIDS patients makes these patients highly susceptible to a variety of opportunistic infections of bacterial, fungal, protozoal or viral etiology. The etiological agents among viral opportunistic infections are often found in the herpes virus group, i.e. herpes simplex virus (HSV), Varicella Zoster virus (VZV), Epstein-Barr virus (EBV) and, especially, cytomegalovirus (CMV). Other retroviruses affecting hwnans are HTLV-I and II and examples of retroviruses affecting animals are feline leukemia virus and equine infectious anaemia vi:rus. Human diseases such as multiple sclerosis, psoriasis, tropical spastic paresis and Kawasaki disease have also been reported to be associated with retrovirus infections.
Hepatitis ~ virus infections cause severe disease such as acute hepatitis, chronic hepatitis, fulminant hepatitis in a considerable number of persons. It is estimated that there are 200 million patients with chronic hepatitis B infection in the world. A considerable number of the chronic cases progress to liver cirrosis and liver tumours. In some cases the hepatitis infections also take a rapid and severe course as in fulminant B
hepatitis with about 90% mortality. At present there is no known effective treatment against hepatitis B infections. The replication of hepatitis B virus is similar to that of retrovirus and it contains the same essential viral reverse transcriptase activity.
... .
.-. ~ , . :
.:
Field of the _nvention The present invention relates to novel chemical compounds and pharmaceutically acceptable salts thereof which can be used in therapy for therapeutic and prophylactic treatment of the acquired immuno deficiency syndrome (AIDS) and infections caused by viruses requiring reverse transcriptase for replication, such as human immuno deficiency viruses and hepatitis B virus, and also for treatment of other virus diseases, such as those of herpes vlruses, diseases which include both common in~ections and neoplastic diseases, i.e. cancer.
Backaround of the invention The effects of viruses on bodily functions ls the end result of changes occurring at the cellular and subcellular levels. The pathogenic changes at the cellular level are different for different combinations of viruses and host cells. While some viruses cause a general destruction (killing) o~ certain cells, other may transform cells into a neoplastic state.
Important common viral infections are herpes dermatitis (including herpes labialis)`, herpes keratitis, herpes genitalis, herpes zoster, herpes encephalitis, inEectious mononucleosis and cytomegalovirus infections all of which are caused by viruses belonging to the herpes virus group. Other important viral diseases are influenza A and B which are caused by influenza A
and B virus, respectively. Another important common viral dis-ease is viral hepatitis and especially hepatitis B virus infec-tions are widely spread. Effective and selective antiviral agents are needed for treatment of these diseases as well as for other diseases caused by viruses.
Several different viruses of both DNA and RNA type have been shown to cause tumors in animals. The effect of cancerogenic chemicals can on animals result in activation of latent tumor viruses. It is possible that tumor viruses are involved in human tumors. The most likely human cases known today are leukemias, sarcomas, breast carcinomas, Burkitt lymphomas, nasophopharyngal carcinomas and cervical c~ncers where RNA tumor viruses and herpes viruses are involved. This makes the search for selective : .
. : ' : ' :"" :' ~
WO~2/06102 2 ~ ~ 3 0 ~ ~ PCT/S~91/0~653 -inhibitors of tumorogenic viruses and their functions an important undertaking in the efforts to treat cancer.
In the late seventies a new disease was reported, which subse~uently was refe~red to as Acquired Immuno Deficiency Syndrome (AIDS). It is now generally accepted that a retrovirus referred to as HIV (Human Immunodeficiency Virus), formerly known as Human T-cell Lymphotrophic Virus (HTLV-III) or Lymphadenopathy Associated Virus (LAV) plays an essential role in the etiology of AIDS. Different types of HIV have been found, such as HIV-l and HIV-2 and more are likely to be isolated.
AIDS is characterized by a profound immunodeficiency due to low numbers of a subset of lymphocyte-T-helper cells, which are one target for HIV infection. The profound immunodeficiency in AIDS patients makes these patients highly susceptible to a variety of opportunistic infections of bacterial, fungal, protozoal or viral etiology. The etiological agents among viral opportunistic infections are often found in the herpes virus group, i.e. herpes simplex virus (HSV), Varicella Zoster virus (VZV), Epstein-Barr virus (EBV) and, especially, cytomegalovirus (CMV). Other retroviruses affecting hwnans are HTLV-I and II and examples of retroviruses affecting animals are feline leukemia virus and equine infectious anaemia vi:rus. Human diseases such as multiple sclerosis, psoriasis, tropical spastic paresis and Kawasaki disease have also been reported to be associated with retrovirus infections.
Hepatitis ~ virus infections cause severe disease such as acute hepatitis, chronic hepatitis, fulminant hepatitis in a considerable number of persons. It is estimated that there are 200 million patients with chronic hepatitis B infection in the world. A considerable number of the chronic cases progress to liver cirrosis and liver tumours. In some cases the hepatitis infections also take a rapid and severe course as in fulminant B
hepatitis with about 90% mortality. At present there is no known effective treatment against hepatitis B infections. The replication of hepatitis B virus is similar to that of retrovirus and it contains the same essential viral reverse transcriptase activity.
... .
.-. ~ , . :
.:
2 0 9 3 0 2 ~ PCT/SE91/00653 General outline of the invention A great number of nucleoside analogues exhibit several antimetabolic activities. They do so by substituting for or competing with the naturally occuring nucleosides. Recently some nucleoside analogues have been described, which inhibit in cell culture the multiplication of human immuno deficiency virus (HIV, also called H~LV-III, LAV) the causative agent of AIDS and AIDS-related complex (ARC). The nucleoside analogues described which inhibit HIV multiplication all have the ~-D-furanosyl configuration.
We have now found that activities for inhibition of HIV
multiplication are exhibited by nucleoside analogues in which the 2'-hydroxyl or 3'-hydroxyl is substituted for ~y a methyl or hydroxymethylene group or another methylene derivative thereof.
The furanosyl moiety may have a D-configuration or L-configuration.
Prior art Some of the compounds and intermediates of the invention have been described previously.
J. Med. Chem. Vol 22, 1979, 518-525 describes the synthesis of methyl 2,3-dideoxy-3-C-(hydroxymethyl)-~-D-erythropento-furanoside as well as the correspondincl dibenzyl- and dibenzoyl derivative. The chloro derivative 5-O-benzoyl-3-C-t(benzoyloxy)methyl)]-2,3-dideoxy-D-erythro-pentafuranosyl chloride was coupled with 2-acetamido-6-chloropurine. The product was processed further to give t~e two anomers of 2-amino-9-[2,3-dideoxy-3-C-3-(hydroxymethyl)-D-erythro-pentofuranosyl]-9H-purine-6(1H)-thione which were tested for antitumor activity. The synthesis of 2 ,3'-dideoxy-3'(R)-hydroxymethyl uridine has been described in Nucleosides &
Nucleotides 1 (1982) 263-273. Uridine was converted to 1-(3-a~ino-2,3-dideoxy-~-D-glucopyranosyl)uracil via a multistage s~quence, which was further reacted via ring contraction, epimerization and reduction to give 2',3'-dideoxy-3'(R)-hydroxymethyl uridine.
- :- . : , ', , ~ . .
WO92/06102 2 ~ ~ ~ 0 2 ~ PCT/SE91/006~3 ~-Anomer compounds of t~e:formula ,HO
~X-~, ~J } ~
~O .
\1/ !
wherein X is O, S or CH2; Y is OH or NH2 and R5 is H, CH3, CH2CH3, CH2CH2CH3, CH(CH3)2, CH=CH2, C-CH or CH=CH-CH3, and their ~-anomers are described in the International Patent Appplication No. PCT/SE88/00169, (publication No. WO 88/08001).
Disclosure of the invention The present invention relates to new compounds OI the formula lA OR lB
R' ~ ~ ~ R~
lA lB
wherein X is O, S, SO, SO2 or CH2; R1 i5 OH, OPO(OH)2, OPO(OH)-O-PO(OH)2, OPO(OH)-O-PO(OH)-o-Po(oH)2 or (CH2)nOCH2 PO(OH)2 wherein n is 0-2; R2 is H and R3 is CH3, CH2OH, CH2OCH3, CH2SH, CH2F or CH2N3; or R3 is H and R2 is CH3, CH2OH, CH2OCH3, CH2SH, CH2F or CH2N3;
Y
~4 iS O ~ r ~
wherein Y is OH, NH2 and R5 is CH=CH2, C_CH, CH=CH-CH3, -C--CH3 thien-2-yl; thien-3-yl, H, CH3, C2H5, n-C3H7 or i-C3H7;
R6 and R7 are the same or different and are H, F, Cl, OH, NH2 : , , .
WO92/06102 2 ~ 9 3 0 2 ~ PCT/SE91/00653 or SH;
and pharmaceutically acceptable salts thereof.
In formula lA the sugar has either the ~-D- or the ~-D-configuration.
~ ~R
lAb ~-D-configuration lAa ~-D-configuration .
In formula lB the sugar has either the ~-L- or the ~-L-configuration.
~ 1 _ ~ R~ ~ R~
lBa ~-L-configuration lBb ~-L-configuration Said compounds have been found to inhibit the multiplication of hu~ 1 immunodeficien¢y virus (HIV).
The invention consequently also re~ers to the compounds of the formulas lA and lB for use in therapy. The compounds of the formulas lA and lB are useful as therapeutic and/or prophylactic agents in the control and treatment of HIV virus infections in man. In a more general aspect, the compounds of the formulas lA
and lB are useful as therapeutic and/or prophylactic agents in the control ~nd treatment sf infections caused by retroviruses and hepatitls B virus in ~ammals and man.
All re~roviruses, including HIV, require the enzyme reverse transcriptase for repli~ation.
Hepatitis B virus (HBV) is a DNA virus with a unique circular double-stranded DNA genome which is partly single-stranded. It contains a specific DNA polymerase required for viral replication. This DNA polymerase also acts as a :. " :' ' ' ' ' '" " ~, ":.,:,, ', ~ . , " . .. :: ,.,' 'W O 92/06102 2 ~ 9 3 ~ 2 ~ PC~r/~E91/00653 reverse transcriptase during the replication of HBV DNA via an RNA intermediate.
Other possible uses of the compounds of formulas lA and lB
are as antimetabolic and antineoplastic compounds and compounds for treatment of other viruses.
One possible use for the compounds of the formulas lA and lB is in the treatment of herpes virus infection. Among the herpes viruses may be mentioned herpes simplex type 1 and 2, varicella (herpes zoster), virus causing infectious mononucleosis (i.e. Epstein-Barr virus), cytomegalovirus and human herpes virus type 6. Important diseases caused by herpes viruses are herpes dermatitis (including herpes labialis), herpes genitalis, herpes keratitis, herpes encephalitis and herpes zoster.
Another possible area of use for the compounds of the present invention is in the treatment of cancer and tumors, particularly those caused by viruses. This effect may be obtained in different ways, i.e. by inhibiting the spread of viruses from transformed cells to o~her normal cells and by arresting the growth of virus-transformed cells.
Another possible area of use for the compounds of the present invention is in the treatment of parasitic infections.
Parasites usually have a specific enzyme activities for nucleoside metabolism making them amenable for therapy by nucleoside analogs. Among the parasites may be mentioned the Schistosama, the Dipetalonema, the Trypanosoma, the Leishmania, the Trichononas Emereia, the Plasmodium and the Toxoplasma families. In this regard, an important member of the Protozoa is I `^`
Pneumocystis carinii, causing severe opportunistic infection in AIDS patients. r The invention furthermore provides:
A pharmaceutical composition comprising a compound of the formulas lA and lB as an active ingredient and a pharmaceutically acceptable carrier, including lipsomes; and A method for therapeutic and/or prophylactic treatment of virus infections in an animal or human host in need of treatment comprising administering an effective amount of a compound of the formulas lA and lB.
It is a preferred aspect of the invention to treat - : ,' ` .- :
, .
WO92/06102 2 ~ 9 3 0 2 ~ PCT/SE91/00653 infections caused by viruses requiring reverse transcriptase for replication, including hu-.an immuno deficiency viruses and hepatitis B virus.
The invention also relates to the use of a compound of the formulas lA and lB for the manufacture of a medicament for therapeutic and/or prophyla~ctic treatment of the acquired immuno deficiency syndrome and infections caused by viruses requiring reverse transcriptase for replication.
Preferably they can be used for the treatment of infections caused by HIV viruses or hepatitis B virus.
The followiny compounds of formulas lA are particularly useful in medical therapy.
Rt R
R~R~
lAb wherein X is O, S and CH2; Rl is OH; R2 is H and R3 is CH2OH; R3 is H and R2 is CH2OH;
R is o~J\ or R ~3~
Y is NH2 and R5 is H; Y is O' and R5 is H, CH3, C-CH, CH - CH-CH3; R6 is NH2 a..d R7 is H, OH, SH or NH2; R6 is H and R7 is OH, SH or NH2; R6 is F or Cl and R7 is OH, SH or NH2; R6 is OH
and R7 is OH.
Examples of pharmaceutically acceptable salts of the compounds of formulas lA and lB include base salts, e.g.
derived from an appropriate base, such as alkali metal (e.g.
sodium, potassium, alkaline earth metal, e.g. magnesium) salts, ~:; ., ; : , . . .
-.: : :.:,, ,, ; ::, :
WO~2/06102 2 ~ 9 3 0 2 C PCT/SE91/006~3 -~
ammonium and tetraalkylammonium. Physiologically acceptable acid salts include salts of organic carboxylic acids such as acetic, lactic, gluconic, citric, tartaric, maleic, malic, pantothenic, isethionic, oxalic, lactoblonic and succinlc acids; organlc sulfonic acids such as methanesulfonic, ethanesulfonic, benzenesulfonlc, p-chlorobenzenesulfonlc and p- toluenesulfonic acids and inorganic acids such as hydrochloric, hydroiodic, sulfuric, phosphoric and sulfamic acids.
Mono~, di- and triphosphate esters of the compounds are also included ln the invention. Physiologically acceptable counterions of the phosphate groups include inorganic and organic counterions. Inorganic counterions are for example ammonium, sodium, potassium, lithium, magnesium and calcium.
Organic counterions are derived from non-to~ic bases, such as primary, secondary and tertiary amines, including naturally occuring amines. Examples of such amines are diethylamine, triethylamine, isopropylamine, ethanolamine, morpholine, 2-diethylaminoethanol, glucosamine, N-methylglucamine, piperazine and dicyclohexylamine.
In clinical practice the nucleoside analogues of the formula I will normally be administered orally, by injection or by in~usion in the form of a pharmaceutical preparation comprising the active ingredient in the form of the original compound or optionally in the form of a pharmaceut:ically acceptable salt thereof, in association with a pharmaceutically acceptable carrier which may be a solid, semi-solid or liquid diluent or an ingestible capsule. The compound may also be used without carrier material. As examples of pharmaceutical preparations may be mentioned tablets, dragées, capsules, granulates, suspensions, elixirs, syrups, solutions, liposomes etc. Usually the active substance will comprise between 0.05 and 20 % for preparations intended ~or injection and between l0 and 90 % for preparations intended for oral administration.
In the treatment of patients suffering from retrovirus, especially HIV, or hepatitis B virus infections, it ~ill be preferred to administer the compounds by any suitable route including the oral, parenteral, rectal, nasal, topical and vaginal route. The parenteral route includes subcutaneous, intramuscular, intravenous and sublingual administration. The ,, ~ .
:, ; .
. ~ ' , .
W O 92/06102 2 ~ 9 3 0 2 0 PCT/SE91/00653 topical route includes buccal and sublingual administration.
The dosage at which the active ~gredients are administered may vary within a wide range and will depend on various factors such as the severity of the infection, the age of the patient etc., and may have to be individually adjusted. As a possible range for the amount of the compounds of the invention or a physiologically acceptable salt thereof to be administered per day may be mentioned from about 10 mg to about 10000 mg, preferentially 50 - 500 mg for intravenous administration and preferentially 50 - 3000 mg for oral administration.
Compounds of the formulas lA and lB can cooperate synergistically or additively with a wide range of other therapeutic agents, thereby enhancing the therapeutic potential of both agents without adding the toxic effects, thus increasing the therapeutic ratio.
Therefore, a compound of formulas lA and lB or a pharmaceutically acceptable derivative thereof can be use in combination therapy, wherein the two active agents are present in a ratio resulting in an optimal therapeutic ratio. This can be provided either by a synergistic effect against the viral infection and/or by a decrease in toxicity while maintaining a therapeutic effect which is additive or synergistic.
The optimal therapeutic ratio is observed when the two agents are present in a ratio of 500:1 to 1:500, preferably 100:1 to 1:100, particularly 20:1 to 1:20 and especially 10:1 to 1:10.
Said combination may conveniently be administered together, for example, in a unitary pharmaceutical formulation, or separately for example as a combination of tablets and injections administered at the same time or at different times, in order to achieve the required therapeutic effect.
The compounds of the formulas lA and lB are potentiated by interferons, other antiviral agents such as foscarnet, AZT, fluorothymidine, dideoxyinosine, dideoxydidehydrothymidine, 9-[4-hydroxy-(2-hydroxymethyl)butyl~guanine, acyclovir, HIV
protease inhibitors, immunomodulators, interferon inducers and growth factors.
Particularly preferred types of interferon are a, b and q interferon inducers.
Other combinations suitable for use according to the present ' , . : :. . . ...
., , , , , , - . . , , . ~ ' ' , ' ~
, :. ! ' -WO9Z/06102 2 ~ ~ 3 0 2 ~ PCT/SE91/00653 ~
invention include those wherein the second agent is, for example, interleukin II, foscarnet esters, inhibitors of HIV
protease such as pepstatin, steroids, medications such as levamisol or thymosin to increase lymphocyte numbers and/or function as appropriate, or G-CSF and other ~actors regulating cell functions.
Methods of PreParation The compounds of the invention may be prepared as outlined below, however, the invention is not limited to these methods.
The compounds may also be prepared by processes described in the known art.
The synthesis of lA where R1 is OH, R2 is H, R3 is CH2OH and R4 is uracil-l-yl has been previously described in Nucleosides and Nucleotides, vol 1, 1982, 263-273. This derivative, suitably protected can be converted to other nucleoside derivatives by processes known in the art such as transglycosylation with suitably derivatized purine or pyrimidine bases or by chemical transformations of uracil.
The synthesis of methyl 2,3-dideoxy-3-C-(hydroxymethyl)-~-D-erythropentafuranoside and methyl 5-0-benzoyl-3-C- r ~(benzoyloxy)methyl]-2,3-dideoxy-~-D-erythro-pentofuranoside, starting from D-glucose, is described in J. Med. Chem., vol. 22, lg79, 518-525.
This latter compound was converted to 5-0-benzoyl-3-C-t(benzoyloxy)methyl]-2,3-dideoxy-D-erythro-pentofuranosyl chloride which was glycosylated with silylated 2-acetamido-6--chloropurine. The carbohydrate part of lA can also be pxepared from a protected 2,3-dideoxy-D-glycero-pent-2-enono-1,4-lactone through a 1,4-Michael type addition with an appropriate carbon nucleophile in an analogous manner as described in Carbohydr.
Res. Vol. 183, 1988, 261 275 and J. org. Chem., Vol. 53, 1988, 4780-4786.
- ~ . . . .
, . .
.: ~
WO92/06102 2 0 9 3 0 2 ~ PCT/SE91/006~3 A compound of the formula R~
~~ R S' Rl3 ~ lA
wherein X, Rl, R2, R3 and R4 are as defined in claim l can be prepared by condensing a glycoside as comprised in the formula to the N-l position of a pyrimidine derivative or to the N-9 position of a purine derivative.
R~' , IA
R3' ~' wherein Z is Cl, Br, J, acyloxy or alkoxy, and Rl, R2~ and R3 are Rl, R2 and R3 respectively, as defi.ned above or:with the proviso that when Rl or R2 is OH then 0 must have a protecting group, R~' is R4 as defined above, havi.ng a silyl, acyl or alkyl protect_ng group A compound of the formula ~ ~ lB
Rl wherein X, R, R2 and R3 are as defined in claim l, can in the same way be prepared by condensing a glycoside as comprised in the formula to the N-l position of a pyrimidine derivative or to the ~-9 position of a p.urine derivative 3~ ~1 ~ lB
Rl - , , : -: : :.: - :: : . : .. ::.. . .
WO92/06102 2 0 9 3 0 2 a 12 PCT~SE91/00653 --wherein Z is Cl, Br, J, acyloxy or alkyloxy and Rl~, R2~ and R3~ are R, R2 and R3 respectively as defined above or with the proviso that when R1 or R2 is OH must have a protecting group, R4~ is R4 as defined above, having a silyl, acyl or alkyl protecting group.
According to a new method the compounds of the formula ~ Z `' ~
Rl wherein X, Rl, R2 and R3 are as defined in claim 1 and Z is Cl, Br, J, aceloxy or alkoxy, can be prepared by reacting a protected butan-1,4-diol-2,3 epoxide with a nucleophile containing 3 or 4 carbon atoms with a double bond, followed by transforming the double bond and ring closure.
Compounds of the type lA can thus be synthesized as outlined below in Scheme 1 or as in Scheme 2-4.
.
- ' ' . '' ' . ~ . ,,, ~ ,., ~
~ ~ , ' , ` .
, .. , -, . ~ ~ , : -. ~
. ' , : .
WO92/06102 2 ~ ~ 3 0 2 ~ PCT/SE91/00653 OF~ G;~ oR9 J ~0."~ ~ .
o,'l I ~ l ' ~ HO
OF~ o~8 0 1 R .p ~omobon2yl 2 R9.. p aromobonzyl 3 H R9~H
R~O
O C H, b lA
ROO . . .
. . . . . _ :
i~ ~.aon~oyl 5 R9.p~tomobon~yl 6 Q .r~ .8~n~oyl .80112t)yl Scheme ~ -This method is particularly useful, since by employing an epoxide with the chirality enantiomeric to that of 1, the L-sugars of the formula lB are also synthesized. When R3 is H, compounds with the different functionalities of R3 are selectively prepared.
By analogous procedures the compounds wherein X is S ~re prepared. S may then be oxidized to SO and SO2.
Compounds wherein X is CH2 can be prepared as follows : , . . . ~ . :.: ., , . .:
:: :~ - :: : :. ~: :~:
;: . ~...... : . -WO92/06102 2 ~ 9 3 0 2 ~ PCT/SE91/00653 -O
o~ OBz ~o~1e o~7 o 8 1 A ~ ~o ~ OBZ
Scheme 2 The chiral epoxide 1 [(2S,3R)-3-~[(4- bromobenzyl)oxy~-methyl]oxirane-2-methanol~ where R9 is p-bromobenzyl and ~R is hydrogen can be prepared as described in ~. Org. Chem. Vol~ 52, 1987, 2596-2598. R9 and/or R8 may be any base stable protecting group such as benzyl or alkyl or a silyl protecting groups.
Epoxide 1 can be treated with allyl magnesium bromide or an allyl anion equivalent to give a mixture of 2 and 3 which are separated by conventional techniques such as chromatography or crystallization. In the case where R8 is hydrogen the free hydroxyl in 2 is at this stage preferably protected with an acyl group, preferably benzoyl. Oxidative cleavage of the double bond and treatment of the product with anhydrous methanol containing a catalytic amount of acid gives 4.
Compound 4 may be coupled to silylated purines or pyrimidines using acid catalysis to give the nucleoside derivative as an ~- and ~-mixture. In 4, when R9 and R8 are not both benzoyl, 4 is preferably converted to this derivative by conventional deblocking and blocking procedures. The ~- and ~-anomeric mixture of nucleosides is preferably deprotected after which the ~- and ~-anomers are separated by conventional techniques such as chromatography or crystallization, to give lA
: - - . . . ..
,' ' .~. , ' "' '~: ' ' ' '~
WO92/06102 ~ 9 ~ ~ 2 a PCT/SE91/0065 as defined earlier in wh~ch Rl and R3 are hydroxyl and hydroxymethyl respective_y.
Compounds of the type lB are prepared entirely analogously by starting with an epoxide corresponding to l but having the opposite chirality.
Compounds wherein X is CH2 are prepared from 7 (Chem. Pharm.
Bull. l988, 36, 15) by protecting the ketone as a ketal and reducing the esters to ethers using lithium aluminium hydride and hydrolyzing the ketal to give 8. Reducticn of the keton gave 9 which was condensed with a suitable purine derivative.
Deprotection gave lA. Compounds o~ the type lB where X is CH2 were synthesized in the same way.
Compounds of the type lA wherein X is O and R2 is CH2OH, CH2F
or CH2N3 are prepared from (S)-4-(tert.-butyldiphenylsilyloxy)-methyl-~-butyrolactone(ll) by acylation with ethyl formate and reduction with sodium borohydride and acylation to give l2 after separation using silica gel column chromatography. Reduction with dialkylaluminium hydride followed by acylation gave 13.
This compound was glycosylated with suitable nucleoside base derivatives.
o - ~ O R O~~ / o ~ ~
'~H OH ~ OAC
ll 2 12 Il ~ 2 ~ OAC IA
OAc R10 = tert.-butyl-diphenyl-silylchloride Scheme 3 W O ~2/06102 2 0 9 3 0 h ~ PCT/SE91/006~3 BnS \ / SBn H - H
~ ~ H - 2 2 BzO ~ ~ HO - - H
OBz C '~2B2 BzO I S
SBn ~ OAC
BzO BzO
16 l7 I
BzO BzO
T
BzO BzO
Bz = benzoyl Bn = benzyl T = thymidine c = ~y~sine Scheme 4 Compounds of the type lA wherein X = S are prepared from 14 by treatment with benzyl mercaptane and stannic chloride to give 15. Ring closure of 15 using triphenylphosphine and triiodoimidazole afforded 16. A portion of compound 16 was condensed with silylated thymine. The rest of compound 16 w~s `
treated with mercuric acetate to give 17 which was condensed with silylated cytosine.
The following illustrates the principle and the adaption of the invention, however, without being limited thereof. Temperature is given in degrees Celsius. Concentrations were performed under diminished pressure (1-2 kPa) at a bath WO~2tO6102 20~ 3 0 2 ~ PCT/SE91/006~3 temperature not exceeding 40C. NMR spectra were measured with a JEOL GX-270 or FX-100 instrument, using D2O or CDC13 solutions.
TMS (for CDC13) and TSP or dioxane (for D2O) were used as internal standards. The shifts are reported in ppm. W
absorption spectra were recorded with a Perkin-Elmer Lamda 5 spectrophotometer. TLC were performed on Merck precoated 60 F- I
2S4 plates. Spots were visualiæed by W light and/or charring with 8~ sulfu-ic acid. Column chromatography was performed using silica gel 60 (0.040-0.063 MM, Merck). HPLC was performed on a prepacked steel column (250 x 25 mm) using Polygosil 60-7, C-18 ~Macherey-Nagel). Organic phases were dried over anhydrous magnesium sulphate. Optical rotations were determined with a Perkin-Elmer 141 polarimeter.
This invention can be illustrated by the following examples:
ExamPle 1-[2',3'-Dideoxy-3'-C-(hydroxymethyl)-~-D-erythro-pentofuranosyl]-cytosine.
A suspension of cytosine (120 mg, 1.08 mmol) and a small chrystal of (NH~)2SO4 in hexamethyldisilazane (2 ml) and trimethylchlorosilane (0.2 ml) was refluxed until a clear solution was obtained. The solution was concentrated in vacuo and co-evaporated with dry xylene. The solid residue was dissolved in dry CH2Cl2 (2 ml) under nitrogen and methyl-5-0-benzoyl-3-~(benzoyloxy)methyl]-2,3-dideoxy-D-erythro-pentofuraosîde (170 mg, 0.46 mmol) was added followed by the addition o~ a t-butyl-dimethylsilyl-triflate (0.22 ml, 0.96 mmol). After 24 h at room temperature the reaction wa~ quenched by the addition of aqueous NaHCO3 (sat.) and the resulting mixture was stirred for 30 minutes. The solution was diluted with CH2C12 and washed with NaHCO3 (sat.), dried, filterei ~nd concentrated to give an anomeric mixture of the protected nucleoside. This mixture was treated with methanolic ammonia (20 ml, sat.) for 24 h at room temperture. After concentration, the residue was dissolved in water and extracted with CH2C12. The aqueous phase was concentrated to a small volume and was applied to a semi-preparative C-18 reversed phase chromatography column and eluted with water containing 2~ methAnol. First the ~-isomer ~. .., : ' !.' ~' ,.," '' ' , '' :
WO92/06102 2 0 n 3 o 2 ~ PCT/SE9t/00653 -18 -;
was collected, followed by the ~isomer. The appropriate fractions were combined and evaporated to give 33 mg of the ~- r anomer (30%) and 27 mg of the ~-anomer (24%). ~-Anomer: [~]2~D: -54 (c 0.3, H2O); W (H20) ~max 272 nm (~ 10894); 1H-NMR t270 MHz, D20): 1.92 (m, J2,a,2,b=13.5 Hz, J2~a,3~= 9 Hz, J2la,1~=6~5 Hz, lH, H-2'a); 2.5 (m, lH, ~-3'); 2-7 ~m~ J2~a 2 b= 13-5 Hz~
J2 a,3 = 8H2~ J2~a,1~ = 6 Hz, lH H- 2'b); 3.67, 3.69 (d and q, overlapping, J6~,3~ = 6-2 Hz, Js~a,s~b = 12-5 Hz, J4~,s~a = 5-3 Hz~
3H, H-6' and H-5'a); 3.85 (q, Js~a,s~b = 12-5 Hz, J4~,5~b = 3 Hz~
lH, H-5'b); 4.28 (m, J3"4, = 8 Hz, J4~,5~a = 5-3 Hz, J4"5,b = 3 Hz, lH, H-4r); 6.1 (d and q, overlapping, J5 6 = 7.3 Hz, J~ 2~ =
6.5 Hz, 2H,H-5 and H-1'); 7-8 (d, J5 6 = 7-3 Hz, lH~ H- 6); 13c-N~R (25.05 MHz, D2O): 36.4 (C-2'); 42.3 (C-3'~; 62.7, 63.6 (C-5r~ C-6r); 84.4, 88.2 (C-1', C-4'); 96.6 (C-5); 141.9 (C-6); 158.1 (C-2); 166.8 (C-4). ~-Anomer: [~]26D: +64 (c 0.27, H20); UV (H20) ~ max: 272 nm (~ 9208); lH-NMR (270 MHz, D20):
2.2-2.46 (m, 3H, H-2; H-3'); 3.68 (d, J3, 6~ = 5.5 Hz, 2H, H-6');
3-76 (dd~ J4~,s~a = 5-5 Hz, J5~a,s~b = 12.5 Hz, lH, ~I-5'a); 3.92 ( I 4 ,5 b 2-9 Hz~ J5~a~5~b = 12-5 Hz, lH, H-5tb); 4.01 (m J3"4, 8-1 Hz~ J5a~,4~ = 5-5 Hz, J5~b,4~ = 2.9 ~z, lH, H-4~);
6.05 t3, J5 6 = 7-3 Hz, lH, H-5); 6-11 (dd, Jl~,2~a = 7-0 Hæ~
J1~ 2~b ~ 4.0 Hz, lH, H-l'); 7-91 (d, J5 6 = 7-3 Hz, lH~ H- 6);
3C-NMR t25.05 MHz, D2O): 36.1 (C-2'); 40.8 (C-3'); 62.7, 63.1 (C-5', C-6'), 84.7, 87.1 (C-1', C-4'); 96.5 (C- 5); 142.2 (C-6); 158.2 (C-2); 166.8 (C-4).
Exam~le 2 9-[2',3' Dideoxy-3'-C-(hydroxymethyl)-~-D-erythro~
pentofuranosyl]-adenosine; and Example 3 9-[2',3'-Dideoxy-3'-C-(hydroxymethyl)-~-D-erythro-pentofuranosyl]-adenosineO
6-chloro-purine (200 mg, 1.3 mmol) was condensed with methyl 5-0-benzoyl-3-[(benzoyloxy)methyl]-2,3- dideoxy~D-erythro-pentofuranoside (280 mg, 0.76 mmol) following the same procedure as described in examples 1 and 2 but using acetonitrile was used instead of CH2C12 as solvent to give an anomeric mixture which after isolation was dissolved in - ~ , : ~, , ' ' 1 WO~2/06102 2 ~ ~ 3 0 ~ ~ PCT/SE91/00653 methanolic ammonia (5 ml) and heated at 100 in a sealed tube.
After 20 h the solvent was removed and the residue dissolved in water and extracted with CH2Cl2. The aqueous layer was concentrated to a small volume and was applied to a semi-preparative C-18 reversed phase chromatography column and eluted with water containing 8 % methanol. First the ~-isomer was eluted, followed by the ~-isomer. The appropriate fractions were combined and evaporated to give 20 mg of the ~-anomer (Example 3) (10 ~) and 40 mg of the ~-anomer (Example 2) (20 %).
~-isomer: [a]26D: +41 (c 0.23, H20); W (H20) ~ax 260 nm (e 12864); 1H-NMR (270 MH2, D20): 2.45 (m, lH, H- 2'a);
2.6 (m, lH, H-3') 2.82 (m, J2'~,1' = 6 Hz, J2~b,3' = 7-8 Hz~
J2~b 2~a = 14 Hz, lH, H-2'b); 3.72 and 3.74 (q and d, overlapping, J5~b,4~ = 5-5 Hz~ J5~b,5-a = 12-5 Hz, J6',3~ = 5-9 Hz!_3H, H-5'b and H-6~); 3.86 ~q, J5~a,4~ = 2-9 Hz, J5~a,s~b = 12-5 Hz~ 1 H~
H-5'a); 4.31 (m, 1 H, H- 4'); 6.35 (m, lH, H-l'); 8.19 (s, lH, H-2); 8.32 (s, lH, H- 8); 13C-NMR (25.05 MHz, D20): 35.5 (C-2');
42.3 (C-3'); 62.5, 63.3 (C-5', C-6'); 83.8, 85.3 (C-l', C-4');
119.4 (C- 5); 140.3 (C-8); 148.9 (C-4); 153.0 (C-2~; 155.9 (C-6).
~-isomer: ~]26D: -17 (c 0.27, H20); W (H?) ~max 260 nm (e 10744); lH-~ R (270 MHz, D20): 2.5 (m, lH, H-3'); 2.67 (m, ~ ~ 2 ); 3-69 (q~ J5~a,4~ = 5-1 Hz, J5~a,5~b = 12.5 Hz, lH, H-5'a); 3.77 (d, J6"3, = 5.5 Hz, 2H, H-6'); 3.87 (q, J5'b 4~ =
2.9 Hz, Js~b 5~a = 12.5 Hz, lH, H-5'b); 4.13 (m, lH, H-4'); 6.34 (m, lH, H-1'); 8.18 (s, lH, H-2); 8.32 (s, lH, H-8). l3C-NMR
(25.05, MHz D2O); 35.7 (C-2'); 41.4 (C-3'); 62.8, 63.4 (C-5',C-6'); 85.0, 85.3 (C-l', C-4'); 119.2 (C-5); 140.5 (C-8);
148.7 (C-4); 153.0 (C-2); 155.8 (C-6).
Example 4 1-~2',3'-Dideoxy-3'-C-(hydroxymethyl)-~-D-erythro-pentofuranosyl]-thymine.
Thymine (150 mg, 1.19 mmol) was condensed with methyl 3-C-[(benzoyloxy)methyll-5-0-p-bromobenzyl-2,3-dideoxy-D-erythro-pentofuranoside (205 mg, 0.47 mmol) following the same procedure as described in Examples 1 and 2 to give an anomeric mixture of the protected nucleoside. The mixture was dissolved in ethanol containing NaHC03 (excess) and was hydrogenated over -. .;
WO~2/06102 2 0 9 3 O t? 3 PCTISE91/006~3 -Pd (10 % on carbon, 1 atm) for 3 hours. After workup the residue was further reacted with methanolic ammonia for 24 h.
After concentration the residue was dissolved in water and extracted with CH2C12. The aqueous layer was concentrated to a small volume and was subjected to semi-preparative C-18 reversed phase column chromatography and eluted with water containing 10 % methanol. First the ~-isomer was eluted, followed by the ~-isomer. The appropriate fractions werë combined and evaporated to give 40 mg of the ~-anomer (33%) and 41 mg of ~-anomer (33%).
~ -Isomer: [~]26D -3.6 (c 0.36, H20); W (H20) ~ax:
268 nm (e 13756); 1H-NMR (270 MHz, D20): 1.89 (d, J = 1.1 HZ, 3H, 5-CH3); 1.96 (m, J2~a,2b~ -2 Hz, J2~a,3~ = 9-9 Xæ~ J2~a,1 = 7.7 Hz, lH, H-2'a); 2.47 (m,_lH, H-3'); 2.6 (m, J2'a,2'b' =
13-2 Hz~ J2~b 3~ = 8-1 Hz, J2~b,1~ - 6-2 Hz, lH, H-2'b); 3.64 and 3.68 (d and q overlapping, Js~a,4~ = 5-5 Hz, J5~a,5~b = 12-1 Hz, J6~ 3~ = 6.1 Hz, 3H, ~-5'a, H- 6'); 3.81 (q, J5'b 4, a 2.9 Hz, Js'b 5~a = 12.1 Hz, H-5'b); 4.24 (m, J3, 4, = 8.4 Hz, ~4~ 5~a =
5.5 Hz, J4~ S~b = 2.9 Hz, lH, H-4); 6.11 (q, Jl' 2~a = 7.7 Hz, Jl' 2~b = 6.2 Hz, lH, H-l'); 7.59 (d, J = 1.1 Hz, lH, ~I-6);
3C-NMR (25.05 MHz, D20): 12.5 (5-CH~); 35.7 tC-2'); 42.7 (C-3'); 62.6, 63.5 (C-5', C-6'); 84.2, 87.3 (C-l', C-4'); 111.6 (C-5); 138.1 (~-6); 152.4 (C-2); 167.3 (C-4).
~ -Isomer: [~]26D: +17.8 (c 0-41, H2O); W (~2) ~max 268 nm (e 8516); lH-NMR (270 MHz, D2O); 1.91 (s, 3H, 5- CH3); 2.3 (m, 2H, H-2'); 2.5 (m, lH, H-3'); 3.69 (d, J3~ 6~ = 5-9 Hz, 2H, H 6 ); 3-76 (q~ J5~b,5~a = 12-4 Hz, J5'b,4~ = 5.1 Hz, lH, H-5'b);
3-9 (q~ J5~a,5~b = 12-4 Hz, J5~a,4~ = 2-9 Hz, lH, H-5'a); 3.99 (m, J4~ a 2-9 Hz~ J4 ,s b = 5-1 ~Z, J4"3, = 8.1 Hz, lH, H-41);
6-14 (q~ ~1',2~a = 4-8 Hz, Jl',2'b = 6-6 Hz, lH, H-l'); 7.73 (d, J = 1.1 Hz, lH, H- 6). 13C-NMR (25.05 MHz, ~2) 12.5 (5-CH3);
35.4 (C-2'); 40.9 (C-3'); 62.8, 62.9 (C-5', C-6'); 84.5, 86.1 (C-l', C- 4'); 111.7 (C-5); 138.3 (C-6); 152.7 (C-2); 167.5 (C-4).
Analogouslv the corresponding nucleoside analogs of the formula lB, having the L-configuration, were synthesized in examples 5-10. The synthetic strategy was identical ~o that one outlined in scheme 1 and described in the detail for the preparation of Examples 1 - 4 and their starting materials. For WO92/06102 2 ~ 3 ~ PCT/SE91/00653 Examples 5 - lO, the starting material corresponding to formula 1 of scheme 1 was:
(2R,3S)-3-[[(4-bromobenzyl)oxy]methyl~oxirane-2-methanol.
Exam~le_5 1-[2',3'-Dideoxy-3'-C-(hydroxymethyl)-~-L~erythro-pentofuranosyl~-cytosine; and Exam~le 6 - 1-[2',3'-Dideoxy-3'-C-(hydroxymethyl)-~-L-erythro-pentofuranosyl~-cytosine.
The ~-anomer had [~]D22: +57,3~ (c 0.61, H~O); W (H2O) ~max 273 nm (~ 7647); lH-NMR (270 ~Hz, D20): 1-92 (m, J2/a,2 b=13 5 Hz~
J2~a,3~= 9 ~Z~ J21a,l'=6l5 Hz, lH, H-2'a); 2.5 (m, lH, H-3'~; 2.7 --(m~ J2~a,2~b= 13-5 Hz, J2~a,3~ = 8H2, J2~a,1~ = 6 Hz~ ~H H- 2'b);
3.67, 3.69 (d and dd, overlapping, J6~ = 6-2 Hz~ Js~a,s~b =
12.5 Hz, J4~ 5~a = 5-3 Hz, 3H, H-6' and H-5'a); 3.85 (dd, J5~a 5~b = 12.5 Hz, J4, 5 ~ = 3 Hz, lH, H-5'b); 4.28 (m, J3, 4, = 8 Hz, J4~,s~a = 5-3 Hz, J4"5,b = 3 Hz, lH, H-4'); 6.1 (d and t, overlapping, J5 6 = 7.3 HZr J~ 2~ = 6.5 Hz, 2H, H-5 and H-1'); 7.8 (d, ~5,6 = 7-3 Hz, lH, H-6); 13c-NMR (25.05 MHz, D20): 36.4 (C-2'); 42.3 (C-3'); 62.7, 63.6 (C-5', C-6'); 84.4, 88.2 (C-l', C-4'); 96.6 (C-5); 141.9 (C-6); 158.1 (C-2); 166.8 (C-4). ~-Anomer: [~]D22: -76.3 (c 1.14 H20); W (H20) ~ max: 273 nm (~
5333); lH-NMR (270 MHz, D20): 2.2-2.46 (m, 3H, H-2; H-3'); 3.68 (d, J~, 6~ = 5-5 Hz, 2H, H-6'); 3-76 (dd, J4 ,s~a = 5-5 Hz~
Js a 5 b = 12.5 Hz, lH, H-5'a); 3-92 (dd, J4~,s~b = 2-9 Hz~ J5~a,s~b = 12.5 Hz, lH, H-5'b); 4-01 (m, J3"4, = 8-1 Hz, Jsa~,4~ = 5-5 Hz, J5~b 4~ = 2.9 Hz, lH, H-4'); 6-05 (3, J5 6 = 7-3 Hz~ lH~ H-5);
6-11 (dd~ Jl~ 2'a = 7-0 Hz, Jl~ 2~b = 4-0 Hz, lH, H-1'); 7.91 (d, J5 6 - 7.3 Hz, lH, H- 6); 13C-NMR (25.05 MHz, D2O): 36.1 (C-2');
40.8 (C-3'); 62.7, 63.1 (C-5', C-6'); 84.7, 87.1 (C-l', C-4');
96.5 (C- 5); 142.2 (C-6); 158.2 (C-2), 166.8 (C-4).
Exam~le 7 9-~2',3'-Dideoxy-3'-C-(hydroxymethyl)-~-L-erythro-pentofuranosyl]-adenosine; and '. . ~, ~', . ,, .' '~
W092/06l02 ~ ~ 3 o ~ ~ PCT/SE91/00653 -~
ExamPle 8 9-[2',3'-Dideoxy-3'-C-(hydroxymethyl)~-L-erythro-pentofuranosyl]-adenosine The ~-anomer: [a]D22: -45,2 (c 0.37, H2O); W (H2O) ~max 260 nm (~ 10987); lH-NMR (270 MHz, D20): 2.45 (m, lH, H- 2'a); 2.6 (m, lH, H-3') 2.82 (m, J2~b,1~ = 6 Hz, J2~b,3~ = 7-8 Hz, J2~b,2~a = 14 Hz, lH, H-2'b); 3.72 and 3.74 (dd and d, o~erlapping, J5~b 4~ =
We have now found that activities for inhibition of HIV
multiplication are exhibited by nucleoside analogues in which the 2'-hydroxyl or 3'-hydroxyl is substituted for ~y a methyl or hydroxymethylene group or another methylene derivative thereof.
The furanosyl moiety may have a D-configuration or L-configuration.
Prior art Some of the compounds and intermediates of the invention have been described previously.
J. Med. Chem. Vol 22, 1979, 518-525 describes the synthesis of methyl 2,3-dideoxy-3-C-(hydroxymethyl)-~-D-erythropento-furanoside as well as the correspondincl dibenzyl- and dibenzoyl derivative. The chloro derivative 5-O-benzoyl-3-C-t(benzoyloxy)methyl)]-2,3-dideoxy-D-erythro-pentafuranosyl chloride was coupled with 2-acetamido-6-chloropurine. The product was processed further to give t~e two anomers of 2-amino-9-[2,3-dideoxy-3-C-3-(hydroxymethyl)-D-erythro-pentofuranosyl]-9H-purine-6(1H)-thione which were tested for antitumor activity. The synthesis of 2 ,3'-dideoxy-3'(R)-hydroxymethyl uridine has been described in Nucleosides &
Nucleotides 1 (1982) 263-273. Uridine was converted to 1-(3-a~ino-2,3-dideoxy-~-D-glucopyranosyl)uracil via a multistage s~quence, which was further reacted via ring contraction, epimerization and reduction to give 2',3'-dideoxy-3'(R)-hydroxymethyl uridine.
- :- . : , ', , ~ . .
WO92/06102 2 ~ ~ ~ 0 2 ~ PCT/SE91/006~3 ~-Anomer compounds of t~e:formula ,HO
~X-~, ~J } ~
~O .
\1/ !
wherein X is O, S or CH2; Y is OH or NH2 and R5 is H, CH3, CH2CH3, CH2CH2CH3, CH(CH3)2, CH=CH2, C-CH or CH=CH-CH3, and their ~-anomers are described in the International Patent Appplication No. PCT/SE88/00169, (publication No. WO 88/08001).
Disclosure of the invention The present invention relates to new compounds OI the formula lA OR lB
R' ~ ~ ~ R~
lA lB
wherein X is O, S, SO, SO2 or CH2; R1 i5 OH, OPO(OH)2, OPO(OH)-O-PO(OH)2, OPO(OH)-O-PO(OH)-o-Po(oH)2 or (CH2)nOCH2 PO(OH)2 wherein n is 0-2; R2 is H and R3 is CH3, CH2OH, CH2OCH3, CH2SH, CH2F or CH2N3; or R3 is H and R2 is CH3, CH2OH, CH2OCH3, CH2SH, CH2F or CH2N3;
Y
~4 iS O ~ r ~
wherein Y is OH, NH2 and R5 is CH=CH2, C_CH, CH=CH-CH3, -C--CH3 thien-2-yl; thien-3-yl, H, CH3, C2H5, n-C3H7 or i-C3H7;
R6 and R7 are the same or different and are H, F, Cl, OH, NH2 : , , .
WO92/06102 2 ~ 9 3 0 2 ~ PCT/SE91/00653 or SH;
and pharmaceutically acceptable salts thereof.
In formula lA the sugar has either the ~-D- or the ~-D-configuration.
~ ~R
lAb ~-D-configuration lAa ~-D-configuration .
In formula lB the sugar has either the ~-L- or the ~-L-configuration.
~ 1 _ ~ R~ ~ R~
lBa ~-L-configuration lBb ~-L-configuration Said compounds have been found to inhibit the multiplication of hu~ 1 immunodeficien¢y virus (HIV).
The invention consequently also re~ers to the compounds of the formulas lA and lB for use in therapy. The compounds of the formulas lA and lB are useful as therapeutic and/or prophylactic agents in the control and treatment of HIV virus infections in man. In a more general aspect, the compounds of the formulas lA
and lB are useful as therapeutic and/or prophylactic agents in the control ~nd treatment sf infections caused by retroviruses and hepatitls B virus in ~ammals and man.
All re~roviruses, including HIV, require the enzyme reverse transcriptase for repli~ation.
Hepatitis B virus (HBV) is a DNA virus with a unique circular double-stranded DNA genome which is partly single-stranded. It contains a specific DNA polymerase required for viral replication. This DNA polymerase also acts as a :. " :' ' ' ' ' '" " ~, ":.,:,, ', ~ . , " . .. :: ,.,' 'W O 92/06102 2 ~ 9 3 ~ 2 ~ PC~r/~E91/00653 reverse transcriptase during the replication of HBV DNA via an RNA intermediate.
Other possible uses of the compounds of formulas lA and lB
are as antimetabolic and antineoplastic compounds and compounds for treatment of other viruses.
One possible use for the compounds of the formulas lA and lB is in the treatment of herpes virus infection. Among the herpes viruses may be mentioned herpes simplex type 1 and 2, varicella (herpes zoster), virus causing infectious mononucleosis (i.e. Epstein-Barr virus), cytomegalovirus and human herpes virus type 6. Important diseases caused by herpes viruses are herpes dermatitis (including herpes labialis), herpes genitalis, herpes keratitis, herpes encephalitis and herpes zoster.
Another possible area of use for the compounds of the present invention is in the treatment of cancer and tumors, particularly those caused by viruses. This effect may be obtained in different ways, i.e. by inhibiting the spread of viruses from transformed cells to o~her normal cells and by arresting the growth of virus-transformed cells.
Another possible area of use for the compounds of the present invention is in the treatment of parasitic infections.
Parasites usually have a specific enzyme activities for nucleoside metabolism making them amenable for therapy by nucleoside analogs. Among the parasites may be mentioned the Schistosama, the Dipetalonema, the Trypanosoma, the Leishmania, the Trichononas Emereia, the Plasmodium and the Toxoplasma families. In this regard, an important member of the Protozoa is I `^`
Pneumocystis carinii, causing severe opportunistic infection in AIDS patients. r The invention furthermore provides:
A pharmaceutical composition comprising a compound of the formulas lA and lB as an active ingredient and a pharmaceutically acceptable carrier, including lipsomes; and A method for therapeutic and/or prophylactic treatment of virus infections in an animal or human host in need of treatment comprising administering an effective amount of a compound of the formulas lA and lB.
It is a preferred aspect of the invention to treat - : ,' ` .- :
, .
WO92/06102 2 ~ 9 3 0 2 ~ PCT/SE91/00653 infections caused by viruses requiring reverse transcriptase for replication, including hu-.an immuno deficiency viruses and hepatitis B virus.
The invention also relates to the use of a compound of the formulas lA and lB for the manufacture of a medicament for therapeutic and/or prophyla~ctic treatment of the acquired immuno deficiency syndrome and infections caused by viruses requiring reverse transcriptase for replication.
Preferably they can be used for the treatment of infections caused by HIV viruses or hepatitis B virus.
The followiny compounds of formulas lA are particularly useful in medical therapy.
Rt R
R~R~
lAb wherein X is O, S and CH2; Rl is OH; R2 is H and R3 is CH2OH; R3 is H and R2 is CH2OH;
R is o~J\ or R ~3~
Y is NH2 and R5 is H; Y is O' and R5 is H, CH3, C-CH, CH - CH-CH3; R6 is NH2 a..d R7 is H, OH, SH or NH2; R6 is H and R7 is OH, SH or NH2; R6 is F or Cl and R7 is OH, SH or NH2; R6 is OH
and R7 is OH.
Examples of pharmaceutically acceptable salts of the compounds of formulas lA and lB include base salts, e.g.
derived from an appropriate base, such as alkali metal (e.g.
sodium, potassium, alkaline earth metal, e.g. magnesium) salts, ~:; ., ; : , . . .
-.: : :.:,, ,, ; ::, :
WO~2/06102 2 ~ 9 3 0 2 C PCT/SE91/006~3 -~
ammonium and tetraalkylammonium. Physiologically acceptable acid salts include salts of organic carboxylic acids such as acetic, lactic, gluconic, citric, tartaric, maleic, malic, pantothenic, isethionic, oxalic, lactoblonic and succinlc acids; organlc sulfonic acids such as methanesulfonic, ethanesulfonic, benzenesulfonlc, p-chlorobenzenesulfonlc and p- toluenesulfonic acids and inorganic acids such as hydrochloric, hydroiodic, sulfuric, phosphoric and sulfamic acids.
Mono~, di- and triphosphate esters of the compounds are also included ln the invention. Physiologically acceptable counterions of the phosphate groups include inorganic and organic counterions. Inorganic counterions are for example ammonium, sodium, potassium, lithium, magnesium and calcium.
Organic counterions are derived from non-to~ic bases, such as primary, secondary and tertiary amines, including naturally occuring amines. Examples of such amines are diethylamine, triethylamine, isopropylamine, ethanolamine, morpholine, 2-diethylaminoethanol, glucosamine, N-methylglucamine, piperazine and dicyclohexylamine.
In clinical practice the nucleoside analogues of the formula I will normally be administered orally, by injection or by in~usion in the form of a pharmaceutical preparation comprising the active ingredient in the form of the original compound or optionally in the form of a pharmaceut:ically acceptable salt thereof, in association with a pharmaceutically acceptable carrier which may be a solid, semi-solid or liquid diluent or an ingestible capsule. The compound may also be used without carrier material. As examples of pharmaceutical preparations may be mentioned tablets, dragées, capsules, granulates, suspensions, elixirs, syrups, solutions, liposomes etc. Usually the active substance will comprise between 0.05 and 20 % for preparations intended ~or injection and between l0 and 90 % for preparations intended for oral administration.
In the treatment of patients suffering from retrovirus, especially HIV, or hepatitis B virus infections, it ~ill be preferred to administer the compounds by any suitable route including the oral, parenteral, rectal, nasal, topical and vaginal route. The parenteral route includes subcutaneous, intramuscular, intravenous and sublingual administration. The ,, ~ .
:, ; .
. ~ ' , .
W O 92/06102 2 ~ 9 3 0 2 0 PCT/SE91/00653 topical route includes buccal and sublingual administration.
The dosage at which the active ~gredients are administered may vary within a wide range and will depend on various factors such as the severity of the infection, the age of the patient etc., and may have to be individually adjusted. As a possible range for the amount of the compounds of the invention or a physiologically acceptable salt thereof to be administered per day may be mentioned from about 10 mg to about 10000 mg, preferentially 50 - 500 mg for intravenous administration and preferentially 50 - 3000 mg for oral administration.
Compounds of the formulas lA and lB can cooperate synergistically or additively with a wide range of other therapeutic agents, thereby enhancing the therapeutic potential of both agents without adding the toxic effects, thus increasing the therapeutic ratio.
Therefore, a compound of formulas lA and lB or a pharmaceutically acceptable derivative thereof can be use in combination therapy, wherein the two active agents are present in a ratio resulting in an optimal therapeutic ratio. This can be provided either by a synergistic effect against the viral infection and/or by a decrease in toxicity while maintaining a therapeutic effect which is additive or synergistic.
The optimal therapeutic ratio is observed when the two agents are present in a ratio of 500:1 to 1:500, preferably 100:1 to 1:100, particularly 20:1 to 1:20 and especially 10:1 to 1:10.
Said combination may conveniently be administered together, for example, in a unitary pharmaceutical formulation, or separately for example as a combination of tablets and injections administered at the same time or at different times, in order to achieve the required therapeutic effect.
The compounds of the formulas lA and lB are potentiated by interferons, other antiviral agents such as foscarnet, AZT, fluorothymidine, dideoxyinosine, dideoxydidehydrothymidine, 9-[4-hydroxy-(2-hydroxymethyl)butyl~guanine, acyclovir, HIV
protease inhibitors, immunomodulators, interferon inducers and growth factors.
Particularly preferred types of interferon are a, b and q interferon inducers.
Other combinations suitable for use according to the present ' , . : :. . . ...
., , , , , , - . . , , . ~ ' ' , ' ~
, :. ! ' -WO9Z/06102 2 ~ ~ 3 0 2 ~ PCT/SE91/00653 ~
invention include those wherein the second agent is, for example, interleukin II, foscarnet esters, inhibitors of HIV
protease such as pepstatin, steroids, medications such as levamisol or thymosin to increase lymphocyte numbers and/or function as appropriate, or G-CSF and other ~actors regulating cell functions.
Methods of PreParation The compounds of the invention may be prepared as outlined below, however, the invention is not limited to these methods.
The compounds may also be prepared by processes described in the known art.
The synthesis of lA where R1 is OH, R2 is H, R3 is CH2OH and R4 is uracil-l-yl has been previously described in Nucleosides and Nucleotides, vol 1, 1982, 263-273. This derivative, suitably protected can be converted to other nucleoside derivatives by processes known in the art such as transglycosylation with suitably derivatized purine or pyrimidine bases or by chemical transformations of uracil.
The synthesis of methyl 2,3-dideoxy-3-C-(hydroxymethyl)-~-D-erythropentafuranoside and methyl 5-0-benzoyl-3-C- r ~(benzoyloxy)methyl]-2,3-dideoxy-~-D-erythro-pentofuranoside, starting from D-glucose, is described in J. Med. Chem., vol. 22, lg79, 518-525.
This latter compound was converted to 5-0-benzoyl-3-C-t(benzoyloxy)methyl]-2,3-dideoxy-D-erythro-pentofuranosyl chloride which was glycosylated with silylated 2-acetamido-6--chloropurine. The carbohydrate part of lA can also be pxepared from a protected 2,3-dideoxy-D-glycero-pent-2-enono-1,4-lactone through a 1,4-Michael type addition with an appropriate carbon nucleophile in an analogous manner as described in Carbohydr.
Res. Vol. 183, 1988, 261 275 and J. org. Chem., Vol. 53, 1988, 4780-4786.
- ~ . . . .
, . .
.: ~
WO92/06102 2 0 9 3 0 2 ~ PCT/SE91/006~3 A compound of the formula R~
~~ R S' Rl3 ~ lA
wherein X, Rl, R2, R3 and R4 are as defined in claim l can be prepared by condensing a glycoside as comprised in the formula to the N-l position of a pyrimidine derivative or to the N-9 position of a purine derivative.
R~' , IA
R3' ~' wherein Z is Cl, Br, J, acyloxy or alkoxy, and Rl, R2~ and R3 are Rl, R2 and R3 respectively, as defi.ned above or:with the proviso that when Rl or R2 is OH then 0 must have a protecting group, R~' is R4 as defined above, havi.ng a silyl, acyl or alkyl protect_ng group A compound of the formula ~ ~ lB
Rl wherein X, R, R2 and R3 are as defined in claim l, can in the same way be prepared by condensing a glycoside as comprised in the formula to the N-l position of a pyrimidine derivative or to the ~-9 position of a p.urine derivative 3~ ~1 ~ lB
Rl - , , : -: : :.: - :: : . : .. ::.. . .
WO92/06102 2 0 9 3 0 2 a 12 PCT~SE91/00653 --wherein Z is Cl, Br, J, acyloxy or alkyloxy and Rl~, R2~ and R3~ are R, R2 and R3 respectively as defined above or with the proviso that when R1 or R2 is OH must have a protecting group, R4~ is R4 as defined above, having a silyl, acyl or alkyl protecting group.
According to a new method the compounds of the formula ~ Z `' ~
Rl wherein X, Rl, R2 and R3 are as defined in claim 1 and Z is Cl, Br, J, aceloxy or alkoxy, can be prepared by reacting a protected butan-1,4-diol-2,3 epoxide with a nucleophile containing 3 or 4 carbon atoms with a double bond, followed by transforming the double bond and ring closure.
Compounds of the type lA can thus be synthesized as outlined below in Scheme 1 or as in Scheme 2-4.
.
- ' ' . '' ' . ~ . ,,, ~ ,., ~
~ ~ , ' , ` .
, .. , -, . ~ ~ , : -. ~
. ' , : .
WO92/06102 2 ~ ~ 3 0 2 ~ PCT/SE91/00653 OF~ G;~ oR9 J ~0."~ ~ .
o,'l I ~ l ' ~ HO
OF~ o~8 0 1 R .p ~omobon2yl 2 R9.. p aromobonzyl 3 H R9~H
R~O
O C H, b lA
ROO . . .
. . . . . _ :
i~ ~.aon~oyl 5 R9.p~tomobon~yl 6 Q .r~ .8~n~oyl .80112t)yl Scheme ~ -This method is particularly useful, since by employing an epoxide with the chirality enantiomeric to that of 1, the L-sugars of the formula lB are also synthesized. When R3 is H, compounds with the different functionalities of R3 are selectively prepared.
By analogous procedures the compounds wherein X is S ~re prepared. S may then be oxidized to SO and SO2.
Compounds wherein X is CH2 can be prepared as follows : , . . . ~ . :.: ., , . .:
:: :~ - :: : :. ~: :~:
;: . ~...... : . -WO92/06102 2 ~ 9 3 0 2 ~ PCT/SE91/00653 -O
o~ OBz ~o~1e o~7 o 8 1 A ~ ~o ~ OBZ
Scheme 2 The chiral epoxide 1 [(2S,3R)-3-~[(4- bromobenzyl)oxy~-methyl]oxirane-2-methanol~ where R9 is p-bromobenzyl and ~R is hydrogen can be prepared as described in ~. Org. Chem. Vol~ 52, 1987, 2596-2598. R9 and/or R8 may be any base stable protecting group such as benzyl or alkyl or a silyl protecting groups.
Epoxide 1 can be treated with allyl magnesium bromide or an allyl anion equivalent to give a mixture of 2 and 3 which are separated by conventional techniques such as chromatography or crystallization. In the case where R8 is hydrogen the free hydroxyl in 2 is at this stage preferably protected with an acyl group, preferably benzoyl. Oxidative cleavage of the double bond and treatment of the product with anhydrous methanol containing a catalytic amount of acid gives 4.
Compound 4 may be coupled to silylated purines or pyrimidines using acid catalysis to give the nucleoside derivative as an ~- and ~-mixture. In 4, when R9 and R8 are not both benzoyl, 4 is preferably converted to this derivative by conventional deblocking and blocking procedures. The ~- and ~-anomeric mixture of nucleosides is preferably deprotected after which the ~- and ~-anomers are separated by conventional techniques such as chromatography or crystallization, to give lA
: - - . . . ..
,' ' .~. , ' "' '~: ' ' ' '~
WO92/06102 ~ 9 ~ ~ 2 a PCT/SE91/0065 as defined earlier in wh~ch Rl and R3 are hydroxyl and hydroxymethyl respective_y.
Compounds of the type lB are prepared entirely analogously by starting with an epoxide corresponding to l but having the opposite chirality.
Compounds wherein X is CH2 are prepared from 7 (Chem. Pharm.
Bull. l988, 36, 15) by protecting the ketone as a ketal and reducing the esters to ethers using lithium aluminium hydride and hydrolyzing the ketal to give 8. Reducticn of the keton gave 9 which was condensed with a suitable purine derivative.
Deprotection gave lA. Compounds o~ the type lB where X is CH2 were synthesized in the same way.
Compounds of the type lA wherein X is O and R2 is CH2OH, CH2F
or CH2N3 are prepared from (S)-4-(tert.-butyldiphenylsilyloxy)-methyl-~-butyrolactone(ll) by acylation with ethyl formate and reduction with sodium borohydride and acylation to give l2 after separation using silica gel column chromatography. Reduction with dialkylaluminium hydride followed by acylation gave 13.
This compound was glycosylated with suitable nucleoside base derivatives.
o - ~ O R O~~ / o ~ ~
'~H OH ~ OAC
ll 2 12 Il ~ 2 ~ OAC IA
OAc R10 = tert.-butyl-diphenyl-silylchloride Scheme 3 W O ~2/06102 2 0 9 3 0 h ~ PCT/SE91/006~3 BnS \ / SBn H - H
~ ~ H - 2 2 BzO ~ ~ HO - - H
OBz C '~2B2 BzO I S
SBn ~ OAC
BzO BzO
16 l7 I
BzO BzO
T
BzO BzO
Bz = benzoyl Bn = benzyl T = thymidine c = ~y~sine Scheme 4 Compounds of the type lA wherein X = S are prepared from 14 by treatment with benzyl mercaptane and stannic chloride to give 15. Ring closure of 15 using triphenylphosphine and triiodoimidazole afforded 16. A portion of compound 16 was condensed with silylated thymine. The rest of compound 16 w~s `
treated with mercuric acetate to give 17 which was condensed with silylated cytosine.
The following illustrates the principle and the adaption of the invention, however, without being limited thereof. Temperature is given in degrees Celsius. Concentrations were performed under diminished pressure (1-2 kPa) at a bath WO~2tO6102 20~ 3 0 2 ~ PCT/SE91/006~3 temperature not exceeding 40C. NMR spectra were measured with a JEOL GX-270 or FX-100 instrument, using D2O or CDC13 solutions.
TMS (for CDC13) and TSP or dioxane (for D2O) were used as internal standards. The shifts are reported in ppm. W
absorption spectra were recorded with a Perkin-Elmer Lamda 5 spectrophotometer. TLC were performed on Merck precoated 60 F- I
2S4 plates. Spots were visualiæed by W light and/or charring with 8~ sulfu-ic acid. Column chromatography was performed using silica gel 60 (0.040-0.063 MM, Merck). HPLC was performed on a prepacked steel column (250 x 25 mm) using Polygosil 60-7, C-18 ~Macherey-Nagel). Organic phases were dried over anhydrous magnesium sulphate. Optical rotations were determined with a Perkin-Elmer 141 polarimeter.
This invention can be illustrated by the following examples:
ExamPle 1-[2',3'-Dideoxy-3'-C-(hydroxymethyl)-~-D-erythro-pentofuranosyl]-cytosine.
A suspension of cytosine (120 mg, 1.08 mmol) and a small chrystal of (NH~)2SO4 in hexamethyldisilazane (2 ml) and trimethylchlorosilane (0.2 ml) was refluxed until a clear solution was obtained. The solution was concentrated in vacuo and co-evaporated with dry xylene. The solid residue was dissolved in dry CH2Cl2 (2 ml) under nitrogen and methyl-5-0-benzoyl-3-~(benzoyloxy)methyl]-2,3-dideoxy-D-erythro-pentofuraosîde (170 mg, 0.46 mmol) was added followed by the addition o~ a t-butyl-dimethylsilyl-triflate (0.22 ml, 0.96 mmol). After 24 h at room temperature the reaction wa~ quenched by the addition of aqueous NaHCO3 (sat.) and the resulting mixture was stirred for 30 minutes. The solution was diluted with CH2C12 and washed with NaHCO3 (sat.), dried, filterei ~nd concentrated to give an anomeric mixture of the protected nucleoside. This mixture was treated with methanolic ammonia (20 ml, sat.) for 24 h at room temperture. After concentration, the residue was dissolved in water and extracted with CH2C12. The aqueous phase was concentrated to a small volume and was applied to a semi-preparative C-18 reversed phase chromatography column and eluted with water containing 2~ methAnol. First the ~-isomer ~. .., : ' !.' ~' ,.," '' ' , '' :
WO92/06102 2 0 n 3 o 2 ~ PCT/SE9t/00653 -18 -;
was collected, followed by the ~isomer. The appropriate fractions were combined and evaporated to give 33 mg of the ~- r anomer (30%) and 27 mg of the ~-anomer (24%). ~-Anomer: [~]2~D: -54 (c 0.3, H2O); W (H20) ~max 272 nm (~ 10894); 1H-NMR t270 MHz, D20): 1.92 (m, J2,a,2,b=13.5 Hz, J2~a,3~= 9 Hz, J2la,1~=6~5 Hz, lH, H-2'a); 2.5 (m, lH, ~-3'); 2-7 ~m~ J2~a 2 b= 13-5 Hz~
J2 a,3 = 8H2~ J2~a,1~ = 6 Hz, lH H- 2'b); 3.67, 3.69 (d and q, overlapping, J6~,3~ = 6-2 Hz, Js~a,s~b = 12-5 Hz, J4~,s~a = 5-3 Hz~
3H, H-6' and H-5'a); 3.85 (q, Js~a,s~b = 12-5 Hz, J4~,5~b = 3 Hz~
lH, H-5'b); 4.28 (m, J3"4, = 8 Hz, J4~,5~a = 5-3 Hz, J4"5,b = 3 Hz, lH, H-4r); 6.1 (d and q, overlapping, J5 6 = 7.3 Hz, J~ 2~ =
6.5 Hz, 2H,H-5 and H-1'); 7-8 (d, J5 6 = 7-3 Hz, lH~ H- 6); 13c-N~R (25.05 MHz, D2O): 36.4 (C-2'); 42.3 (C-3'~; 62.7, 63.6 (C-5r~ C-6r); 84.4, 88.2 (C-1', C-4'); 96.6 (C-5); 141.9 (C-6); 158.1 (C-2); 166.8 (C-4). ~-Anomer: [~]26D: +64 (c 0.27, H20); UV (H20) ~ max: 272 nm (~ 9208); lH-NMR (270 MHz, D20):
2.2-2.46 (m, 3H, H-2; H-3'); 3.68 (d, J3, 6~ = 5.5 Hz, 2H, H-6');
3-76 (dd~ J4~,s~a = 5-5 Hz, J5~a,s~b = 12.5 Hz, lH, ~I-5'a); 3.92 ( I 4 ,5 b 2-9 Hz~ J5~a~5~b = 12-5 Hz, lH, H-5tb); 4.01 (m J3"4, 8-1 Hz~ J5a~,4~ = 5-5 Hz, J5~b,4~ = 2.9 ~z, lH, H-4~);
6.05 t3, J5 6 = 7-3 Hz, lH, H-5); 6-11 (dd, Jl~,2~a = 7-0 Hæ~
J1~ 2~b ~ 4.0 Hz, lH, H-l'); 7-91 (d, J5 6 = 7-3 Hz, lH~ H- 6);
3C-NMR t25.05 MHz, D2O): 36.1 (C-2'); 40.8 (C-3'); 62.7, 63.1 (C-5', C-6'), 84.7, 87.1 (C-1', C-4'); 96.5 (C- 5); 142.2 (C-6); 158.2 (C-2); 166.8 (C-4).
Exam~le 2 9-[2',3' Dideoxy-3'-C-(hydroxymethyl)-~-D-erythro~
pentofuranosyl]-adenosine; and Example 3 9-[2',3'-Dideoxy-3'-C-(hydroxymethyl)-~-D-erythro-pentofuranosyl]-adenosineO
6-chloro-purine (200 mg, 1.3 mmol) was condensed with methyl 5-0-benzoyl-3-[(benzoyloxy)methyl]-2,3- dideoxy~D-erythro-pentofuranoside (280 mg, 0.76 mmol) following the same procedure as described in examples 1 and 2 but using acetonitrile was used instead of CH2C12 as solvent to give an anomeric mixture which after isolation was dissolved in - ~ , : ~, , ' ' 1 WO~2/06102 2 ~ ~ 3 0 ~ ~ PCT/SE91/00653 methanolic ammonia (5 ml) and heated at 100 in a sealed tube.
After 20 h the solvent was removed and the residue dissolved in water and extracted with CH2Cl2. The aqueous layer was concentrated to a small volume and was applied to a semi-preparative C-18 reversed phase chromatography column and eluted with water containing 8 % methanol. First the ~-isomer was eluted, followed by the ~-isomer. The appropriate fractions were combined and evaporated to give 20 mg of the ~-anomer (Example 3) (10 ~) and 40 mg of the ~-anomer (Example 2) (20 %).
~-isomer: [a]26D: +41 (c 0.23, H20); W (H20) ~ax 260 nm (e 12864); 1H-NMR (270 MH2, D20): 2.45 (m, lH, H- 2'a);
2.6 (m, lH, H-3') 2.82 (m, J2'~,1' = 6 Hz, J2~b,3' = 7-8 Hz~
J2~b 2~a = 14 Hz, lH, H-2'b); 3.72 and 3.74 (q and d, overlapping, J5~b,4~ = 5-5 Hz~ J5~b,5-a = 12-5 Hz, J6',3~ = 5-9 Hz!_3H, H-5'b and H-6~); 3.86 ~q, J5~a,4~ = 2-9 Hz, J5~a,s~b = 12-5 Hz~ 1 H~
H-5'a); 4.31 (m, 1 H, H- 4'); 6.35 (m, lH, H-l'); 8.19 (s, lH, H-2); 8.32 (s, lH, H- 8); 13C-NMR (25.05 MHz, D20): 35.5 (C-2');
42.3 (C-3'); 62.5, 63.3 (C-5', C-6'); 83.8, 85.3 (C-l', C-4');
119.4 (C- 5); 140.3 (C-8); 148.9 (C-4); 153.0 (C-2~; 155.9 (C-6).
~-isomer: ~]26D: -17 (c 0.27, H20); W (H?) ~max 260 nm (e 10744); lH-~ R (270 MHz, D20): 2.5 (m, lH, H-3'); 2.67 (m, ~ ~ 2 ); 3-69 (q~ J5~a,4~ = 5-1 Hz, J5~a,5~b = 12.5 Hz, lH, H-5'a); 3.77 (d, J6"3, = 5.5 Hz, 2H, H-6'); 3.87 (q, J5'b 4~ =
2.9 Hz, Js~b 5~a = 12.5 Hz, lH, H-5'b); 4.13 (m, lH, H-4'); 6.34 (m, lH, H-1'); 8.18 (s, lH, H-2); 8.32 (s, lH, H-8). l3C-NMR
(25.05, MHz D2O); 35.7 (C-2'); 41.4 (C-3'); 62.8, 63.4 (C-5',C-6'); 85.0, 85.3 (C-l', C-4'); 119.2 (C-5); 140.5 (C-8);
148.7 (C-4); 153.0 (C-2); 155.8 (C-6).
Example 4 1-~2',3'-Dideoxy-3'-C-(hydroxymethyl)-~-D-erythro-pentofuranosyl]-thymine.
Thymine (150 mg, 1.19 mmol) was condensed with methyl 3-C-[(benzoyloxy)methyll-5-0-p-bromobenzyl-2,3-dideoxy-D-erythro-pentofuranoside (205 mg, 0.47 mmol) following the same procedure as described in Examples 1 and 2 to give an anomeric mixture of the protected nucleoside. The mixture was dissolved in ethanol containing NaHC03 (excess) and was hydrogenated over -. .;
WO~2/06102 2 0 9 3 O t? 3 PCTISE91/006~3 -Pd (10 % on carbon, 1 atm) for 3 hours. After workup the residue was further reacted with methanolic ammonia for 24 h.
After concentration the residue was dissolved in water and extracted with CH2C12. The aqueous layer was concentrated to a small volume and was subjected to semi-preparative C-18 reversed phase column chromatography and eluted with water containing 10 % methanol. First the ~-isomer was eluted, followed by the ~-isomer. The appropriate fractions werë combined and evaporated to give 40 mg of the ~-anomer (33%) and 41 mg of ~-anomer (33%).
~ -Isomer: [~]26D -3.6 (c 0.36, H20); W (H20) ~ax:
268 nm (e 13756); 1H-NMR (270 MHz, D20): 1.89 (d, J = 1.1 HZ, 3H, 5-CH3); 1.96 (m, J2~a,2b~ -2 Hz, J2~a,3~ = 9-9 Xæ~ J2~a,1 = 7.7 Hz, lH, H-2'a); 2.47 (m,_lH, H-3'); 2.6 (m, J2'a,2'b' =
13-2 Hz~ J2~b 3~ = 8-1 Hz, J2~b,1~ - 6-2 Hz, lH, H-2'b); 3.64 and 3.68 (d and q overlapping, Js~a,4~ = 5-5 Hz, J5~a,5~b = 12-1 Hz, J6~ 3~ = 6.1 Hz, 3H, ~-5'a, H- 6'); 3.81 (q, J5'b 4, a 2.9 Hz, Js'b 5~a = 12.1 Hz, H-5'b); 4.24 (m, J3, 4, = 8.4 Hz, ~4~ 5~a =
5.5 Hz, J4~ S~b = 2.9 Hz, lH, H-4); 6.11 (q, Jl' 2~a = 7.7 Hz, Jl' 2~b = 6.2 Hz, lH, H-l'); 7.59 (d, J = 1.1 Hz, lH, ~I-6);
3C-NMR (25.05 MHz, D20): 12.5 (5-CH~); 35.7 tC-2'); 42.7 (C-3'); 62.6, 63.5 (C-5', C-6'); 84.2, 87.3 (C-l', C-4'); 111.6 (C-5); 138.1 (~-6); 152.4 (C-2); 167.3 (C-4).
~ -Isomer: [~]26D: +17.8 (c 0-41, H2O); W (~2) ~max 268 nm (e 8516); lH-NMR (270 MHz, D2O); 1.91 (s, 3H, 5- CH3); 2.3 (m, 2H, H-2'); 2.5 (m, lH, H-3'); 3.69 (d, J3~ 6~ = 5-9 Hz, 2H, H 6 ); 3-76 (q~ J5~b,5~a = 12-4 Hz, J5'b,4~ = 5.1 Hz, lH, H-5'b);
3-9 (q~ J5~a,5~b = 12-4 Hz, J5~a,4~ = 2-9 Hz, lH, H-5'a); 3.99 (m, J4~ a 2-9 Hz~ J4 ,s b = 5-1 ~Z, J4"3, = 8.1 Hz, lH, H-41);
6-14 (q~ ~1',2~a = 4-8 Hz, Jl',2'b = 6-6 Hz, lH, H-l'); 7.73 (d, J = 1.1 Hz, lH, H- 6). 13C-NMR (25.05 MHz, ~2) 12.5 (5-CH3);
35.4 (C-2'); 40.9 (C-3'); 62.8, 62.9 (C-5', C-6'); 84.5, 86.1 (C-l', C- 4'); 111.7 (C-5); 138.3 (C-6); 152.7 (C-2); 167.5 (C-4).
Analogouslv the corresponding nucleoside analogs of the formula lB, having the L-configuration, were synthesized in examples 5-10. The synthetic strategy was identical ~o that one outlined in scheme 1 and described in the detail for the preparation of Examples 1 - 4 and their starting materials. For WO92/06102 2 ~ 3 ~ PCT/SE91/00653 Examples 5 - lO, the starting material corresponding to formula 1 of scheme 1 was:
(2R,3S)-3-[[(4-bromobenzyl)oxy]methyl~oxirane-2-methanol.
Exam~le_5 1-[2',3'-Dideoxy-3'-C-(hydroxymethyl)-~-L~erythro-pentofuranosyl~-cytosine; and Exam~le 6 - 1-[2',3'-Dideoxy-3'-C-(hydroxymethyl)-~-L-erythro-pentofuranosyl~-cytosine.
The ~-anomer had [~]D22: +57,3~ (c 0.61, H~O); W (H2O) ~max 273 nm (~ 7647); lH-NMR (270 ~Hz, D20): 1-92 (m, J2/a,2 b=13 5 Hz~
J2~a,3~= 9 ~Z~ J21a,l'=6l5 Hz, lH, H-2'a); 2.5 (m, lH, H-3'~; 2.7 --(m~ J2~a,2~b= 13-5 Hz, J2~a,3~ = 8H2, J2~a,1~ = 6 Hz~ ~H H- 2'b);
3.67, 3.69 (d and dd, overlapping, J6~ = 6-2 Hz~ Js~a,s~b =
12.5 Hz, J4~ 5~a = 5-3 Hz, 3H, H-6' and H-5'a); 3.85 (dd, J5~a 5~b = 12.5 Hz, J4, 5 ~ = 3 Hz, lH, H-5'b); 4.28 (m, J3, 4, = 8 Hz, J4~,s~a = 5-3 Hz, J4"5,b = 3 Hz, lH, H-4'); 6.1 (d and t, overlapping, J5 6 = 7.3 HZr J~ 2~ = 6.5 Hz, 2H, H-5 and H-1'); 7.8 (d, ~5,6 = 7-3 Hz, lH, H-6); 13c-NMR (25.05 MHz, D20): 36.4 (C-2'); 42.3 (C-3'); 62.7, 63.6 (C-5', C-6'); 84.4, 88.2 (C-l', C-4'); 96.6 (C-5); 141.9 (C-6); 158.1 (C-2); 166.8 (C-4). ~-Anomer: [~]D22: -76.3 (c 1.14 H20); W (H20) ~ max: 273 nm (~
5333); lH-NMR (270 MHz, D20): 2.2-2.46 (m, 3H, H-2; H-3'); 3.68 (d, J~, 6~ = 5-5 Hz, 2H, H-6'); 3-76 (dd, J4 ,s~a = 5-5 Hz~
Js a 5 b = 12.5 Hz, lH, H-5'a); 3-92 (dd, J4~,s~b = 2-9 Hz~ J5~a,s~b = 12.5 Hz, lH, H-5'b); 4-01 (m, J3"4, = 8-1 Hz, Jsa~,4~ = 5-5 Hz, J5~b 4~ = 2.9 Hz, lH, H-4'); 6-05 (3, J5 6 = 7-3 Hz~ lH~ H-5);
6-11 (dd~ Jl~ 2'a = 7-0 Hz, Jl~ 2~b = 4-0 Hz, lH, H-1'); 7.91 (d, J5 6 - 7.3 Hz, lH, H- 6); 13C-NMR (25.05 MHz, D2O): 36.1 (C-2');
40.8 (C-3'); 62.7, 63.1 (C-5', C-6'); 84.7, 87.1 (C-l', C-4');
96.5 (C- 5); 142.2 (C-6); 158.2 (C-2), 166.8 (C-4).
Exam~le 7 9-~2',3'-Dideoxy-3'-C-(hydroxymethyl)-~-L-erythro-pentofuranosyl]-adenosine; and '. . ~, ~', . ,, .' '~
W092/06l02 ~ ~ 3 o ~ ~ PCT/SE91/00653 -~
ExamPle 8 9-[2',3'-Dideoxy-3'-C-(hydroxymethyl)~-L-erythro-pentofuranosyl]-adenosine The ~-anomer: [a]D22: -45,2 (c 0.37, H2O); W (H2O) ~max 260 nm (~ 10987); lH-NMR (270 MHz, D20): 2.45 (m, lH, H- 2'a); 2.6 (m, lH, H-3') 2.82 (m, J2~b,1~ = 6 Hz, J2~b,3~ = 7-8 Hz, J2~b,2~a = 14 Hz, lH, H-2'b); 3.72 and 3.74 (dd and d, o~erlapping, J5~b 4~ =
5-5 Hz, J5~b,5~a = 12-5 Hz, J6~,3~ = 5-9 Hz, 3H, H-5'b and H-6');
3-86 (q~ Js~a,4~ ~ 2-9 Hz, Js~a,5~b = 12.S Hz, 1 H, H-5'a); 4.31 (m, 1 H, H- 4'); 6.35 (m, lH, H-l'); 8.19 (s, lH, H-2); 8.32 (s, lH, H- 8); 13C-NMR (25.05 MHz, D20): 35.5 (C-2'); 42.3 (C-3');
62.5, 63.3 (C-5~, C-6'); 83.8, 85.3 (C-l', C-4'); 119.4 (C- 5);
140.3 (C-8); 148.9 (C-4); 153.0 (C-2); 15S.9 (C-6).
THe ~-anomer: [a]:D22 +22.5 (c 0-44! H2O); W (H2O) ~max 260 nm (~ 11482); lH-NMR (270 MHz, D20): 2.5 (m, lH, H-3'); 2.67 (m, 2H, H-2~); 3.69 (dd, Js~a,4~ = 5-1 Hz, Js~a~s~b = 12-5 Hz~ lH~
H-5'a); 3.77 (d, J6~ 3~ = 5.5 Hz, 2H, H-6'); 3.87 {dd, J5~b 4~ =
2.9 Hz, Js~b 5~a = 12.5 Hz, lH, H-5'b); 4.13 (m, lH, H-4'); 6.34 (m, lH, H-l'~; 8.18 (s, lH, H-2); 8.32 (s, lH, H-8). 13C-NMR
(25.05 MHz D20); 35.7 (C-2'); 41.4 (C-3'); 62.8, 63.4 (C-5' and C~6'); 85.0, 85.3 (C-l'and C-4'); 119.2 (C 5); 140.5 (C-8);
148.7 (C-4); 153.0 (C-2); 155.8 (C-6).
Exam~le g 1-[2',3'-Dideoxy-3'-C-(hydroxymethyl)-~-L-erythro-pentofuranosyl]-thymine and Exam~le 10 1-[2',3'-Dideoxy-3'-C-(hydroxymethyl)-~-L-erythr~-pento~uranosyl]-thymine The ~-anomer had [~]D22 +8,3 (c 0.48, H203; W (H2O) ~maX
268 nm (~ 7976); lH-NMR (270 MXz, D~O): 1.89 (d, J = 1.1 Hz , 3H, 5-CH3); 1.96 (m,J2,a,2,b=13-2 Hz, J2~a,3~= 9-9 Hz, J2~a,1~=7~7 Hz, lH, H-2'a); 2.47 (m, lH, H-3'); 2-6 (m, J2~at2~b= 13-2 Hz~
J2 b,3, = 8-1 Hz, J2,b,1 .= 6-2 Hz, lH H- 2'b); 3.64, 3.68 (d and dd, overlapping, J5.,4, = 5-5 Hz, J5~a,5~b = 12-1 Hz~ J6~,3~ = 6-1 Hz, 3H, H-6'a and H-6'); 3-81 (dd, Js,~,s~ = 2-9 Hz, J5~b,5~a =
12.1 Hz, H-5'b); 4-24 (m, J3"4 = 8-4 Hz, J4~,5~a = 5-5 Hz~
J4, 5,~ = 2.9 Hz, lH, H-4); 6-11 (dd, Jl~,2~a = 7-7 Hz, J 1,2 b =
.: . : ........ . ......... .
: ', , . ' ' - .
,: :, . : " : , WO92/06l02 ~ ~ 3 3 ~ 2 ~ PCT/SE91/006~3 6.2 Hz, lH, H-l); 7.59 (d, J = 1.1 Hz, lH, H-6); 13c-NMR (25.05 t MHz, D2O): 12.5 (5-CH3) 35.7 (C-2'); 42.7, (C-3') 62.6, 63.5 (C-5'and C-6'); 84.2, 87.3 (C-l' and C-4'); 111.6 (C-5); 138.1 (C-6); 152.4 ~C-2); 167.3 (C-4).
The ~-anomer: [~]D2 : -21.2 (c 0.32 H20); ~ max: 268 nm (~
8123); lH-NMR (270 MHZ, D20): 1.91 (s, 3H, 5-CH3);
2.3 (m, 2H, H-2'); 2.5 (m, = lH, H-3'); 3.69 (d, J3~ 6~ = 5 9 ~z 2H, H-6 ); 3-76 (dd~ J~5b,5~a = 12-4 Hz, J5 b,4 = 5.1 Hz, lH, H-5 b); 3-9 (dd~ J5~a,s~b = 12-4 H2, J5a~,4~ = 2.9 Hz, lH, H5,a); =
3-99 (m~ J4~,5~a = 2-9 HZ~ (J4',5~b = 5-1 Hz, J4"3, = 8.1 Hz, lH, H 4); 6-14 (dd~ Jl~,2~a = 4-8 HZ~ Jl ,2 b = 6-6 Hz, lH~ ~-1); 7 73 (d, J = 1.1 Hz 1~, H-6). 13C-NMR (25.05 MHz, D2O): 12.5 (5-CH3), 35.4 (C-2'); 40.g (C-3'); 62.8, 62.9 (C-5' and C-6'); 84.5, 86.1 (C-l'and C-4'); 111.7 (C 5); 138.3 (C-6); 152.7 (C-2); 167.5 (C-4).
General procedure for the silylationc used in Examples 11-1~ A suspensi~n consisting of the base (1 mmol) and a small crystal of ammonium sulfate in a mixture of hexamethyldisilazane (2 ml) and trimethylchlorosilane (0.2 ml) was refluxed until a clear solution was obtained. Volatile matters were evaporated o~f, and the residue was repeatedly coevaporated with added xylene.
1-[2',3'-Dideoxy-3'-C-(fluoromethyl)-~- and B-D-erythrG-pentofuranosyl]-thymine.
Thymine (150 mg, 1.19 mmol) was silylated according to the general procedure and dissolved in lichloromethane (5 ml) under nitrogen. To this solution was added methyl 5-0-benzoy~-3-C-(fluoromethyl)-2,3-dideoxy-D-erythro-pentofuranoside (200 mg, 0.75 mmol) followed by tert-butyldimethylsilyl triflate (0.3 ml, 1.31 mmol). The solution was stirred for 24 hours at room temperature, after which the reaction was quenched by the addition of aqueous sodium hydrogen carbonate, stirred for 30 min, diluted with dichloromethane, washed with aqueous sodium hydrogen carbonate, dried, and concentrated t~ give an anomeric mixture of the protected nucleosides. The mixture was treated with methanolic ammonia (20 ml, saturated) for 24 h at room temperature. ~fter concentration to dryness, the rasidue was "
: :
. . :
WO92/06102 2 ~ ~ 3 0 2 ~ PCT/SE91/00653 --dissolved in water and washed with dichloromethane. The aqueous layer was concentrated to a small volume and the mixture was separated by column chromatography (ethyl acetate-methanol 20:1). The B~anomer was eluted first followed by the ~-anomer.
The appropriate fractions were combined and evaporated to give the title compounds, ~ (70 mg, 36 %) and ~ (54 mg, 28 %). :
[~2~D -13.1 (c 0.82, H20); W (H2O) ~max 268 nm (~ 9268); lH NMR
(270 MHz, D20) ~ 2.92 (5-CH3), 2.06 (H-2'a), 2.60-2.82 (m, H-2'b and H-3'), 3.68 (dd, H-5'a), 3.85 (dd, H-5'b), 4.38 (m, H-4), 4.46-4.75 (2 m, H-6'a-b), 6.15 (t, H-l'), 7.60 (d, H-6).
Anal. Calcd. for Cl1HlsO4N2F: C, S1.16; H, 5.85; N, 10.85. Found:
C, 50.91; H, 5.78; N, 10.85. B: t~]22D ~16.3 (c 0.6, H20); W
(H2O) ~max 268 nm (~ 8880); 1H NMR (270 MHz, D2O) ~ 1.91 (d, 5-CH3), 2.3 (m, H-2'), 2.73 (m, H-3'), 3.78 (dd, H-5'a), 3.94 (dd, J4~ 5 b = 2.9 Hz, H-5'b), 4.1 (m, H-4), 4.44-4.73 (2 m, H-6'a,b), 6.15 (dd, H-1'), 7.77 (d, J = 1.1 Hz, H-6). Anal. Calcd for Cl1H1504N2F: c, 51.16; H, 5.85; N, 10.85. Found: C, 51.10; H, 5.76; N, 10.95.
EX~MPLE 12 1-[2',3'-Dideoxy-3'-C-(fluoromethyl)-~-and-~-D-erythro-pentofuranosyl]cytosine.
Cytosine ~200 mg, 1.8 mmol) was silylated according to the general procedure and the synthesis~ was proceded as described above to give the title compounds ~ (38 mg, 38 %) and ~ (26 mg, 26 %). ~ [~]22D ~53-7 (c 0.94, H20); W (H2O) ~max 272 nm ~ 7762); 1H NMR (270 MHz, D20) ~ 1.98 (m, H-2'a), 2.58-2.80 (m, H-2'b and H-3'), 3.68 (H-5'a), 3.85 (dd, H-5'b), 4.37 (m, H-4), 4.39-4.72 (2 m, H-6'a,b), 6.04 (d, H-5), 6.12 (t, H-1'), 7.70 (d, H-6). Anal. Calcd. for CloH14O3N3F: C, 49.38; H, 5.80.
Found: C; H. ~ [~]2~D +44.8 (c 0.6, H20); W (H2O) ~max 272 nm (~
8515); lH N~R (270 MHz, D20) ~ 2.Z3 (m, H-2'a), 2.39 (m, J2'b 3~ =
3-86 (q~ Js~a,4~ ~ 2-9 Hz, Js~a,5~b = 12.S Hz, 1 H, H-5'a); 4.31 (m, 1 H, H- 4'); 6.35 (m, lH, H-l'); 8.19 (s, lH, H-2); 8.32 (s, lH, H- 8); 13C-NMR (25.05 MHz, D20): 35.5 (C-2'); 42.3 (C-3');
62.5, 63.3 (C-5~, C-6'); 83.8, 85.3 (C-l', C-4'); 119.4 (C- 5);
140.3 (C-8); 148.9 (C-4); 153.0 (C-2); 15S.9 (C-6).
THe ~-anomer: [a]:D22 +22.5 (c 0-44! H2O); W (H2O) ~max 260 nm (~ 11482); lH-NMR (270 MHz, D20): 2.5 (m, lH, H-3'); 2.67 (m, 2H, H-2~); 3.69 (dd, Js~a,4~ = 5-1 Hz, Js~a~s~b = 12-5 Hz~ lH~
H-5'a); 3.77 (d, J6~ 3~ = 5.5 Hz, 2H, H-6'); 3.87 {dd, J5~b 4~ =
2.9 Hz, Js~b 5~a = 12.5 Hz, lH, H-5'b); 4.13 (m, lH, H-4'); 6.34 (m, lH, H-l'~; 8.18 (s, lH, H-2); 8.32 (s, lH, H-8). 13C-NMR
(25.05 MHz D20); 35.7 (C-2'); 41.4 (C-3'); 62.8, 63.4 (C-5' and C~6'); 85.0, 85.3 (C-l'and C-4'); 119.2 (C 5); 140.5 (C-8);
148.7 (C-4); 153.0 (C-2); 155.8 (C-6).
Exam~le g 1-[2',3'-Dideoxy-3'-C-(hydroxymethyl)-~-L-erythro-pentofuranosyl]-thymine and Exam~le 10 1-[2',3'-Dideoxy-3'-C-(hydroxymethyl)-~-L-erythr~-pento~uranosyl]-thymine The ~-anomer had [~]D22 +8,3 (c 0.48, H203; W (H2O) ~maX
268 nm (~ 7976); lH-NMR (270 MXz, D~O): 1.89 (d, J = 1.1 Hz , 3H, 5-CH3); 1.96 (m,J2,a,2,b=13-2 Hz, J2~a,3~= 9-9 Hz, J2~a,1~=7~7 Hz, lH, H-2'a); 2.47 (m, lH, H-3'); 2-6 (m, J2~at2~b= 13-2 Hz~
J2 b,3, = 8-1 Hz, J2,b,1 .= 6-2 Hz, lH H- 2'b); 3.64, 3.68 (d and dd, overlapping, J5.,4, = 5-5 Hz, J5~a,5~b = 12-1 Hz~ J6~,3~ = 6-1 Hz, 3H, H-6'a and H-6'); 3-81 (dd, Js,~,s~ = 2-9 Hz, J5~b,5~a =
12.1 Hz, H-5'b); 4-24 (m, J3"4 = 8-4 Hz, J4~,5~a = 5-5 Hz~
J4, 5,~ = 2.9 Hz, lH, H-4); 6-11 (dd, Jl~,2~a = 7-7 Hz, J 1,2 b =
.: . : ........ . ......... .
: ', , . ' ' - .
,: :, . : " : , WO92/06l02 ~ ~ 3 3 ~ 2 ~ PCT/SE91/006~3 6.2 Hz, lH, H-l); 7.59 (d, J = 1.1 Hz, lH, H-6); 13c-NMR (25.05 t MHz, D2O): 12.5 (5-CH3) 35.7 (C-2'); 42.7, (C-3') 62.6, 63.5 (C-5'and C-6'); 84.2, 87.3 (C-l' and C-4'); 111.6 (C-5); 138.1 (C-6); 152.4 ~C-2); 167.3 (C-4).
The ~-anomer: [~]D2 : -21.2 (c 0.32 H20); ~ max: 268 nm (~
8123); lH-NMR (270 MHZ, D20): 1.91 (s, 3H, 5-CH3);
2.3 (m, 2H, H-2'); 2.5 (m, = lH, H-3'); 3.69 (d, J3~ 6~ = 5 9 ~z 2H, H-6 ); 3-76 (dd~ J~5b,5~a = 12-4 Hz, J5 b,4 = 5.1 Hz, lH, H-5 b); 3-9 (dd~ J5~a,s~b = 12-4 H2, J5a~,4~ = 2.9 Hz, lH, H5,a); =
3-99 (m~ J4~,5~a = 2-9 HZ~ (J4',5~b = 5-1 Hz, J4"3, = 8.1 Hz, lH, H 4); 6-14 (dd~ Jl~,2~a = 4-8 HZ~ Jl ,2 b = 6-6 Hz, lH~ ~-1); 7 73 (d, J = 1.1 Hz 1~, H-6). 13C-NMR (25.05 MHz, D2O): 12.5 (5-CH3), 35.4 (C-2'); 40.g (C-3'); 62.8, 62.9 (C-5' and C-6'); 84.5, 86.1 (C-l'and C-4'); 111.7 (C 5); 138.3 (C-6); 152.7 (C-2); 167.5 (C-4).
General procedure for the silylationc used in Examples 11-1~ A suspensi~n consisting of the base (1 mmol) and a small crystal of ammonium sulfate in a mixture of hexamethyldisilazane (2 ml) and trimethylchlorosilane (0.2 ml) was refluxed until a clear solution was obtained. Volatile matters were evaporated o~f, and the residue was repeatedly coevaporated with added xylene.
1-[2',3'-Dideoxy-3'-C-(fluoromethyl)-~- and B-D-erythrG-pentofuranosyl]-thymine.
Thymine (150 mg, 1.19 mmol) was silylated according to the general procedure and dissolved in lichloromethane (5 ml) under nitrogen. To this solution was added methyl 5-0-benzoy~-3-C-(fluoromethyl)-2,3-dideoxy-D-erythro-pentofuranoside (200 mg, 0.75 mmol) followed by tert-butyldimethylsilyl triflate (0.3 ml, 1.31 mmol). The solution was stirred for 24 hours at room temperature, after which the reaction was quenched by the addition of aqueous sodium hydrogen carbonate, stirred for 30 min, diluted with dichloromethane, washed with aqueous sodium hydrogen carbonate, dried, and concentrated t~ give an anomeric mixture of the protected nucleosides. The mixture was treated with methanolic ammonia (20 ml, saturated) for 24 h at room temperature. ~fter concentration to dryness, the rasidue was "
: :
. . :
WO92/06102 2 ~ ~ 3 0 2 ~ PCT/SE91/00653 --dissolved in water and washed with dichloromethane. The aqueous layer was concentrated to a small volume and the mixture was separated by column chromatography (ethyl acetate-methanol 20:1). The B~anomer was eluted first followed by the ~-anomer.
The appropriate fractions were combined and evaporated to give the title compounds, ~ (70 mg, 36 %) and ~ (54 mg, 28 %). :
[~2~D -13.1 (c 0.82, H20); W (H2O) ~max 268 nm (~ 9268); lH NMR
(270 MHz, D20) ~ 2.92 (5-CH3), 2.06 (H-2'a), 2.60-2.82 (m, H-2'b and H-3'), 3.68 (dd, H-5'a), 3.85 (dd, H-5'b), 4.38 (m, H-4), 4.46-4.75 (2 m, H-6'a-b), 6.15 (t, H-l'), 7.60 (d, H-6).
Anal. Calcd. for Cl1HlsO4N2F: C, S1.16; H, 5.85; N, 10.85. Found:
C, 50.91; H, 5.78; N, 10.85. B: t~]22D ~16.3 (c 0.6, H20); W
(H2O) ~max 268 nm (~ 8880); 1H NMR (270 MHz, D2O) ~ 1.91 (d, 5-CH3), 2.3 (m, H-2'), 2.73 (m, H-3'), 3.78 (dd, H-5'a), 3.94 (dd, J4~ 5 b = 2.9 Hz, H-5'b), 4.1 (m, H-4), 4.44-4.73 (2 m, H-6'a,b), 6.15 (dd, H-1'), 7.77 (d, J = 1.1 Hz, H-6). Anal. Calcd for Cl1H1504N2F: c, 51.16; H, 5.85; N, 10.85. Found: C, 51.10; H, 5.76; N, 10.95.
EX~MPLE 12 1-[2',3'-Dideoxy-3'-C-(fluoromethyl)-~-and-~-D-erythro-pentofuranosyl]cytosine.
Cytosine ~200 mg, 1.8 mmol) was silylated according to the general procedure and the synthesis~ was proceded as described above to give the title compounds ~ (38 mg, 38 %) and ~ (26 mg, 26 %). ~ [~]22D ~53-7 (c 0.94, H20); W (H2O) ~max 272 nm ~ 7762); 1H NMR (270 MHz, D20) ~ 1.98 (m, H-2'a), 2.58-2.80 (m, H-2'b and H-3'), 3.68 (H-5'a), 3.85 (dd, H-5'b), 4.37 (m, H-4), 4.39-4.72 (2 m, H-6'a,b), 6.04 (d, H-5), 6.12 (t, H-1'), 7.70 (d, H-6). Anal. Calcd. for CloH14O3N3F: C, 49.38; H, 5.80.
Found: C; H. ~ [~]2~D +44.8 (c 0.6, H20); W (H2O) ~max 272 nm (~
8515); lH N~R (270 MHz, D20) ~ 2.Z3 (m, H-2'a), 2.39 (m, J2'b 3~ =
8.5 Hz, H-2'b), 2.65 (m, H-3'), 3.77 (dd, H-5'a), 3.93 (dd, H-5'b), 4.11 (m, H-4), 4.43-4.73 (two m, H-6'a,b), 6.03 (d, H-5), 6.10 (dd, H-l'), 7.93 (d, H-6). Anal. Calcd. for CloH1403N3F: C, 49,38; H, 5.80. Found: C; H.
, : . . : . . . .: , : .
::: : : . .
:: ~ : ;. :. ::
-, , ;
WO92/06102 2 ~ 3 3 ~ 2 ~ PCT/SE91/00653 9-[2',3'-Dideoxy-3'-C (fluoromethyl)-~-and-B-D-erythro-pentofuranosyl]adenine. 6-Chloropurine (120 mg, 0.78 mmol) was silylated according to the general procedure and the synthesis was proceeded as described above to give the title compounds ~
(25 mg, 26 %) and B (37 mg, 39 %). a: [~22D 35.2 (c 0.71, H20);
W (H2O) ~maX 260 nm (~ 10239); 1H NMR (270 MHz, D20) ~ 2.5 (m, H-2'a), 2.70-2.95 (m, H-Z'b and H-3'), 3.73 (dd, H-5'a), 3.38 (dd, H 5'b) 4 40 (m H-4), 4.52-4.78 (2 m, J3~,6~b = 10-0 Hz, H
b), 6.34 (t, H-l'), 8.15 (s, H-2), 8.29 (s, H-8). Anal. Calcd.
for C~1H14O2NsF: C, 49.43; H, 5.28. Found: C; H. B: [~)22D -24.3 (c 0.9, H20); W (H20) ~maX 260 nm (~ 10364); lH NMR (270 MHz, D20) ~ 2.47-2.48 (2 m, H-2'a,b), 2.85 (m, H-3'), 3.69 (dd, H-5'a), 3.87 (dd, H-5'b), 4.21 (m, H-4), 4.51-4.78 (2 m, H-6'a,b), 6.24 (dd, H-l'), 8.04 (s, H-2), 8.25 (s, H-8). Anal. Calcd. for C11H1402N5F: C, ~9-43; H, 5.28. Found: C,; H,.
1-[2',3'-Dideoxy-3'-C-(azidornethyl)-~-and-B-D-erythro-pentofuranosyl]-thymine.
Thymine (133 m~ 1.06 mmol) was silylated according to the general procedure an dissolved in dichloromethane (5 ml) under nitrogen. To this solution was added methyl 3-C
(azidomethyl)-5-O-(benzoyl)-2',3'-dideoxy-D-erythro-pentofuranoside (154 mg, 0.53 mmol) followed by tert-butyldimethylsilyl triflate (0.25 ml, 1.09 mmol). The solution was stirred for 24 hours at room temperature, after which the reaction was quenched by the addition of aqueous sodium hydrogen carbonate, stirred for 30 min, diluted with dichloromethane, washed with aqueous sodium hydrogen carbonate, dried, and concentrated to give an anomeric mixture of the protected nucl~osides. The mixture was treated with mPthanolic ammonia (20 ml, saturated) for 24 h at room temperature. After concentration to dryness, the residue was dissolved in water and washed with dichlorome~hane. The aqueous layer was concentrated to a small volume and freeze dried to give 110 mg (0.39 mmol, 74 %) of an anomeric mixture of the deblocked nucleosides. The mixture was dissolved in a mixture of N,N-dimethylformamid and imidazole (53 mg, 0.78 mmol) followed by the addition of tert-butyldimethyl-. .
~-': . :
WO92/06102 ~93 ~ 2 ~ PCT/SE91/00653 silyl chloride (70 mg, 0.46 mmol), and the solution was stirred for 3 h at room temperature. Water (1 ml) was added, and the mixture was stirred for 10 min, diluted with dichloromethane, washed with 1 M hydrogen chloride, saturated aqueous hydrogen carbonate, dried, and concentrated. The anomers were separated by column chromatography (hexane-ethyl acetate 1:1), the ~-anomer was eluted first followed by the B-anomer. Each of the anomers was separately treated with tetrabutyl-ammonium fluoride (70 mg, 0.22 mmol) in tetrahydrofuran (2 ml) for 24 h, concentrated, the residue dissolved in water, and washed with dichloromethane. The aqueous layer was concentrated to a small volume and purified bv HPLC (water-methanol, 80:20, v/v) to give the title compounds ~ (43 mg, 29 %) and ~ (44 mg, 30 %). ~:
[~]22D +3.1 (c 1.45, H2O); W (H2O) ~max 268 nm (~ 9155); lH NMR
(270 MHz, D2O) ~ 1.92 (s, 5-CH3), 2.02 (m, H-2'a), 2.50-2.74 (2 m, H-2'b and H-3'), 3.54 and 3.58 (2 dd, H-6'a,b), 3.67 tdd, H-5'a), 3.85 (dd, H-5'b), 4.25 (m, J4~ 5~a = 5.1 Hz, H-4), 6.12 (dd, H-1), 7.60 (d, H-6). Anal. Calcd. for Cl1Hl504N2F: C, 51.16;
H, 5.85. Found: C.; H,. ~ [~]22D 12.5 (c 0.96, H2O~; W (H2O) ~max 268 nm (~ 9434); lH NMR (270 MHz, D20) ~ 1.91 (s, 5-CH3), 2.34 (dd, H-2'), 2.59 (m, H-3'), 3.47-3.61 (2 dd, H-6'a,b), 3.78 (dd, H-5'a), 3.94 (dd, H-5'b), 3.98 (m, H-4), 6.1 (t, H-1), 7.7G
(s, lH, H-6). Anal. Calcd. for CllHl504N5: C, 46.97; H, 5.38.
Found: C,; H,.
1-[2',3'-Dideoxy-3'-C-(azidomethyl)-~-and-B-D-erythro-pentofuranosyl]cytosine.
Cytosine (250 mg, 2.25 mmol) was silylated according to the general procedure and the synthesis was proceeded as aboYe to give the title compounds ~ (84 mg, 43 ~) and B (45 mg, 23 %).
~ [~22D -46.1 (c 1.15~ H20); W (H2O) ~max 27~ nm (~ 8320); H
N~R (270 MHz, D20) ~ 1.96 (m, H-2'a), 2.56 (m, H-3'), 2.74 (m, H-2'b), 3.46-3.58 (2 dd, H-6'a,b), 3.68 (dd, H-5'a), 3.85 (dd, H~
5'b), 4.25 (m, H-5), 6.09 (t, H-1), 7.79 (d, H-6). Anal. Calcd.
for CloH1403N6: C, 45.10; H, 5.30; N, 31.57. Found: C, 4~.~7; H, 5.13; N, 31.37. ~ [~]22D 55.00 (c o.99, H20); W (H2O) ~m~x 272 nm (~ 8209); 1H NMR (270 MHz, D2O) ~ 2.22-2.58 (m, H-2' and H-3'), 3.48-3.61 (2 dd, H-6'), 3.79 (dd, H-5'a), 3.94 (dd, H-5'b), 4.0 ..... .
'. . ' : ., :
~3023~
WO92/06102 PCT/S~91/00653 (m, H-4'), 6.04 (d, H-~), 6.11 (dd, H-1'), 7.93 (d, H-6). Anal.
Calcd- for C1oHl4O3N6: _, 45-10; H, 5.30. Found: C,; H .
9-~2',3'-Dideoxy-3'-C-(azidomethyl)-~ and B-D-erythro-pento~uranosyl]-adenine.
6-chloropurine (360 mg, 2.33 mmol) was silylated according to the general procedure and the synthesis was proceeded as above to give the title compounds ~ (38 mg, 17 ~) and B (39 mg, 17 %). An analytical sample of each of the anomers was obtained by HPLC purification (water-methanol, 70:30, v/v).
~: [a]22D +58.3 (c 1.0, H20); W (~2) ~max 260 nm (~ 9353); 1H
~NR 270 MHz, D20) ~ 2.43 (m, H-2'a), 2.67 (m, H-3'), 2.8~
(m,H-2'b), 3.61 (d, H-6'), 3.73 (dd, H-5'a), 3.88 (dd,h-5'a), 4.28 (m, H-4'), 6.28 (t, H-1'), 8.11 (s, H-2), 8.28 (s, H-8).
Anal. Calcd for C11Hl4O2N8: C, 45.51; Gm 4.86; N, 38.61. Found:
C, 45.31; Gm 4.80; N, 38.30. B. [~22D -27.6 (c 1.05, H2O; W
(H2O) ~max - nm (~ 14102); 1H NMR 270 MHz, D2O) ~ 2.52 (m, H-2'a), 2.75 (m, H-2'b, H-3'), 3.61 ~dd, H-6'a), 3.64 (dd, J3 6~b = 6.2 Hz, H-6'b), 3.?1 (dd, H-5'a, 3.88 (dd, H-5'b), 4.12 (m, H-4'), 6.36 (dd, and 3.0 Hz, H-1'), 8.19 (s, H-2), 8.33 (s, H-8). Anal. Calcd for CllH14O2N8: C, 45.51; H, 4.86; N, 38.61.
Found: C, 45.28; H, 4.97; N, 38.36.
Example 17 9-[3S,4S)-3,4-di(hydroxymethyl)cyclopentyl~-guanosine To a white suspension of PPh3 (528 mg, 2.0 mmol) and 2-NH2-6-Cl-purin (342 mg, 2.0 mmol in dry THF (14 ml) at room temperature under nitrogen, was added DEAD (Diethylazodicarboxylate) (0.33 ml, 2.3 mmol) and the yellowish suspension was stirred for an additional 4 h. 3,4-dibenzoyloxymethyl-cyclopentanol (200 mg, 0.57 mmol) in dry THF (5 ml) was added and the mixture was stirred for an additional 30 min. The solvent was evaporated and the crude residue was purified by silica gel column chromatography (toluene-ethyl acetate, l:l). The crude product was dissolved in MeOH (2 ml), aqeous NaOH(lM, 2ml) was added dropwise, and the mixture was stirred at 80C for 4 h. After cooling, the reaction mixture was ~ :; . .
-WO92/06102 2 Q 9 3 0 2 ~ PCT/SE9l/00653 -neutralized with aqeous HCl (2M), the aqeous phase was extracted several times with THF-Ethylacetate (1:1) and the solvents were evaporated. The crude product was purified by silica gel column chromatography (chloroform-methanol, 6:1). Recrystallisation from water afforded the title compound in a yield of 57 g (36%).
Exam~le 18 1-(2',3'-Dideoxy-2'-C-hydroxymethyl-~-D-erythro-pentofuranosyl)-thymine A mixture of thymine (253 mg, 2.01 mmol), chlorotrimethylsilane (0.300 ml) and some crystals of (NH4)2SO4 in hexamethyldisilazane (4 ml) was refluxed under nitrogen for 6 h. The clear solution was concentrated and residual volatiles were co-evaporated with added toluene (5 ml). The residue was dissolved in a mixture of dichloromethane-acetonitrile (9:1, 10 ml) and a solution of 2-C-acetoxymethyl-l-O-acetyl-5-O-(tert-b~ltyldiphenyl)silyl-D-erythro-pentofuranose (630 mg, 1.34 mmol) in the same solvent mixture as above (5 ml) was added. The solution was cooled on an ice bath an~ tert-butyldimethylsilyl triflate (0.370 ml, 1.16 mmol) was added dropwise. The mixture wc~s stirred for 30 min on an ice bath followed by 15 h at room temperature. Pyridine (2 ml) was adde~3 and the mixture was iltered through a pad of silica gel. The filtrate was concentrated and the residue purified by column chromatography (toluene-acetone 1:1) to yield 1-(2'-C-acetoxymethyl-5'-O-tert-butyldiphenylsilyl-2',3'-dideoxy-B-D-erytro-pentofuranosyl)-thymine. 100 mg, 0.186 mmol of this material was dissolved in 1 M tetrabutylammonium fluoride x 3 H2O in THF (9 ml) and stirred for 20 min at room temperature. The solution was concentrated and the residue purified by column chromatography (toluene-acetone 1:1). The product was dissolved in methanol (4 ml) and methanol saturated with ammonia (2 ml) was added. The solution was stirred over night, concentrated and the residue purified by column chromatography (chloroform-methanol 9:1) yielding the title compound (38 mg): ~]D +9.1 (c 0.70, H?O): 13C NMR (D2O, 40C) 12.3 (CH3, thymine), 29.2 (C-3'), 46.7 (C-2'), 62.4, 63.8 (C-5', C-6'), ~1.0 (C-4'), 88.8 (C-l'), 112.2 (C-5, 138.6 (C-6), 152.5 (C~4), 167.2); lH NMR (D2O, 40C) 1.90 (s, 3H, CH3, thymine), 2.09 (m, 2H, H-3', H-3''), 2.64 (m, lH, H-2'),3.76 (m, , WO~/0~102 209302a PCT/SE91/006~3 4H, H-5', H-5'', H-6', H-6''), 4031 (m, H-4'), 5.93 (d, J=5.13 Hz, lH, H-1'), 7.68 (s, lH, H-6).
Anal. Calcd. for Cl1H1605N2o C, 51.56; H, 6.29; N, 10.
93. Found: C, 51.28; H, 6.11; N, 10.71.
1-(2',3'-Dideoxy-2/-C-hydroxymethyl-B-D-erythro-pentofuranosyl)cytosine was synthesizied analogous to Example 12 ;
using cytosine instead of thymine yielding (41 mg): [~]D + 31.2 tc 1.20, H20) 13C NMR (D20, 40C) 29.2 (C-3'), 47.6 (C-2'), 62.5, 64.0 (C-5', C-6'), 81.2 (C-4'), 89.8 (C-l'), 96.8 (C-5), 142.6 (C-6). 158.3 (C-4), 166.9 (C-2); lH NMR (D2O, 40C) 2.04 (m, 2H, H-3', H-3''), 2.55 (m, lH, H-2'), 3.77 (m, 4H, H-5', H-5'', H-6', H-6''), 4.33 (m, lH, H-4'), 5.91 (d, J=4.77 Hz, lH, H-1'), 6.05 (d, J= 7.32 ~z, lH, H-5), 7.85 (d, J= 7.32 ~z, lH, H-6)-Anal. Calcd. for CloHl504N3 x H20: C, 46.33; H, 6.61; N, 16.21. Found: C, 46.41; H, 6.31; N, 16.16.
2',3'-Dideoxy-2'-C-hydroxymethyladenosirle was synthesized analogous to Example 12 u~;ing adenine instead of thymine yielding (20 mg): ~]D -10.3 (C 0.20, H2O): W (H20~
~max ~60 nm; 13C NMR (D20, 40C) 29.4 ~C-3'), 46.8 (C-2'), 62.2, (C-5', C-6'), 81.4 (C-4'), 88.3 (C-1'), 141.2-169-4 (5ArC); lH
NMR (D20, 40C) 2.21 (m, 2H, H-3', H-3''~, 3.06 (m, lH, H-2'), 3.76 (m, 4H, H-5'', H-6', H-6''), 4.45 (m, lH, H-4'), 6.07 (d, J= 5.32 Hz, lH, H-1'), 8.15, 8.30 (s, s, 2H, H-2, H-8).
Anal. Calcd. for C11H1503N5: C, 49081; ~, 5.70; N, 26.40. Found: C, 50.01; H, 5.54; N, 26.35.
1-(2'-C-Azidomethyl-2',3'-dideoxy-~-D-erytro-pentofuranosyl)-thymine.
A solution 1-(2'-C-acetoxymethyl-5'-0-tert-butyldiphenyl-2',4'-dideoxy-~ D-erytro-pentofuransyl)thymine (440 mg, 0.820 mmol) in methanol saturated with ammonia (15 ml) was stirred over night. The mixture was concentrated and the residue purified by column chromatography (toluene-acetone 1 '" . '` . ' ' ' ,..
: . .: . : . :.
:,, ., :, ~,;~ ' , ': '' ~
WO92/06l02 2 Q ~ 3 0 ~ ~ PCT/SE91/00653 --The residue (170 mg, 344 mmol) was dissolved in pyridine (5 ml) and methanesulfonyl chloride (0.030 ml, 379 mmol) was added.
After 1 h at room temperature, the solution was concentrated and the residue co-evaporated with added toluene. The residue was purified by column chromatogrphy (toluene-acetone 2:1) yielding 1-(5'-O-tert-butyldiphenylsilyl-2'-C-methanesulfonylmethyl-2',3'-dideoxy-~-D-erythro-pentofuranosyl)thymine which was dissolved in DMF (2 ml). Sodium azide (72 mg, 1.11 mmol) was added and the resulting suspension was stirred at 60C for 3 h.
The mixture was concentrated and purified by column chromatography (toluene-acetone 2:1) (15~ mg) (IR(CHCl3) 2100 cm~
1), which was dissolved in 1 M tetrabutylammonium fluoride x 3 H2O in THF (10 ml) at room temperature. After 10 min, the mixture was concentrated and the residue purified by column chromatography (tsluene-acetone 1:1) yielding the title compound (72 mg). ~] D -31.3 (c 0.76, CHCl3): IR (CHCl3) 2100 cm-1 (azide): 13C NMR (CDCl3) 12.5 (CH3, thymine), 29.6 (C-3'), 44.5 (C-3), 52.0 (C-6'), 63.8 (C-5'), 79.8 (C-4'), 89.0 (C-1'), 111.0 (C-5). 136.6 (C-6), 150.9 (C-4), 164.4 (C-2); 1H NMR (CDCl3) 1.89 (s, 3H, CH3, thymine), 1.96 (m, lH, H-3'), 2.28 (m, lH, H-3''), 2.51 (m, lH, H-2'), 3.53, 3.82 (m, m, 4H, H-5', H-5'', H-6', H-6''), 4.29 (m, Ih, H-4') S.81 (cl, J=5.50 Hz, lH, Hl', 7.~6 (s, lH, H-6), 9.90 (s, lH, H-3).
Anal. Calcd. for C11H1504Ns: C, 46.97; H, 5.38; N, 24.90. Found: C, 46.83; H, 5.21; N, 2~L.71.
2'-C-Azidomethyl-2',3'-dideoxycytidine A solution of 2'-C-acetoxymethyl-5'-O-tert-butyldiphenyl-2',3'-dideoxy-cytidine (260 mg, 0.489 mmol) in methanol saturated with ammonia (10 ml) was stirred over night at room temperature. The mixture was concentrated and the residue purified by column chromatography (chloroform-methanol 9:1) to give 395 mg of the de-o-acetylated cytidine analogue. To a stirred solution of this material (100 mg, 0.208 mmol) in pyridine (3 ml) was added methanesulfonyl chloride (0.020 ml, 0.250 mmol). After 1 h the solution was concentrated and the residue was coevaporated with added toluene. The residue was purified by column chromatography (chloroform-methanol 9:1) (110 -:
: : .
wo 92/06102 2 0 ~ 3 0 2 ~ PCT/SE91/00653 mg) 13C N~R (CDCl3~ 37.3 (CH3), 69.7 (C-6'); 1H NMR (CDCl3) 3.08 (s, 3H, CH3), 4.50 (d, J=4.21 Hz, 2H, H-6', H-6''). 100 mg, 0.179 mmol of this compound was dissolved in DMF (2 ml) and sodium azide (41 mg, 0.628 mmol) was added. The resulting suspension was stirred at 60C for 3.5 h. The mixture was concentrated and the residue purified by column chromatography (toluene-acetone 2:1) (84 mg), (IR(CHC13) 2100 cm-l) this was dissolved in lM tetrabutylammonium fluoride x 3 H20 in THF (4 ml) at rot temperature. After 15 min, the mixture was concentrated and the residue purified by column chromatography (chloroform-methanol 5:1) and then HPLC (reversed phase, methanol-water 85:15) yielding the title compound (36 mg): ~] D
+54.4 (c 0.95, H20): IR(CHC12) 2100 cm~l, (azide): 13C N~R (D20, 40 C) 29.9 _(C-3'), 44.9 (C-2'), 52.6 (C-6', 64.0 (C-5'), 80.8 (C-4r), 90.0 (C-1'), 97.1 C-5), 142.6 (C-6), 158.3 (C-4), 166.9 (C 2); lH NMR (D~C, 40 C) 2.03 (m, 2H, H-3', H-3''), 2.61 (m, lH, H-2'), 3.56 (m, 2H, H-6', H-6''), 3.75 (m, 2H, H-5', H-5''), 4.33 (m, lH, H-4'), 5.92 (d, J= 5.50 Hz, lH, H-1'), 6.05 (d, J=7.32 Hz, lH, H-5), 7.82 (d, J=7.32 Hx, lH, H 6).
Anal. Calcd. for CloHl4O3N6: 45.11; H, 5.30; N, 31.56.
Found: C, 45.02; H, 5.41; N, 31.28.
~_~MPLE 23 1-[5'-O-Benzoyl-3'-C-~(benzoyloxy)methyl]-2',3'-dideoxy-4'-thio-~-and-~-D-erythro-pentafuranosyl]thyminet18).
A suspension of thymine (150~ mg, 1.19 ~mol) and a small crysta_ of ammonium sulfate in a mixtur2 of hexamethyldisilazane (2 ml) and trimethylchlorosilane (0.2 ml) was refluxed until a clean solution was obtained. Volatile matters were evaporated off and the residue was repeatedly co-evaporated with toluene. The resulting syrup was dissolved in dichloromethane (2 ml) under nitrogen. To this solution compound l6 (153 mg, 0.32 mmol) was added followed by the addition of tert.-butyldimethylsilyl triflate (0.075 mL, 0.33 mmol), mercuric ace ate (0.102 g, 0.32 mmol). The solution was stirred for 24 h at room temperature. The reaction was quenched by the addition of saturated aqueous sodium hydrogen carbonate, dried, concentrated and purified by column chromatograp~y (chloroform-ethyl acetate 1:1) to give tO.127 g, 87 ~!. NMR indicated ~hat .
. ,;
'.
W092/06102 2 0 9 3 0 2 ~ PCT/SE91/OOfiS3 -^
coupling of thymine had occurred but the spectra were too complex to analyse without separating the anomers. This material was de-O-benzoylated using sodium in methanol at room temperature to give 1(3'-C-hydroxymethyl)-2',3'-dideoxy 4'-thio-~-and-~-D-erythro-pentofuranosyl)thymine.
1-[5'-0-Benzoyl-3'-C-[(benzoyloxy)methyl]-2',3'-dideoxy-4'-thio-~-and-r~-D-erythro-pentofuranosyl]cytosine.
Cytosine (100 mg, O.gO1 mmol) was silylated following the same procedure as for the preparation of 18 and dissolved in acetonitrile (3 ml) under nitrogen. Compound 17 (119 mg, 0.287 mmol) was added followed by the addition of tert.-~~~~ butyldimethylsilyl triflate (0;13 ml, 0.574 mmol) and thesolution was stirred for 1 h at ooc. The reaction was quenched by the addition of saturated aqueous sodium hydrogen carbonate, stirred for 30 min, diluted with dichloromethane, washed with saturated aqueous sodium hydrogen carbonate, dried, concentrated and purified by column chromatography (ethyl acetate-methanol 4:1) to give an anomeric mixture of the protected nucleoside 15 (49 mg, 37 %). NMR indlcated that coupling of cytosine had occured but the spectra were too complex to analyse without separating the anomers. This material was de-O-benzoylated usinq sodium in methanol at roo~ temperature to give 1(3'-C-hydroxy~.ethyl)-2'-,3'-dideoxy 4'-thio-~-and-~-D-erythr~-pentofuranosyl)cytosine.
Ex~rimental The starting materials for the compounds in Exam~les 1-4 were prepared by the following sequence of reactions a - d:
a) (2S,3R)-l-O-p-Bromobenzyl-3-C-(2'-propenyl)-1,2,4-butantriol (compound 2, Scheme 1). To a cold solution of allylmagnesium bromide -50C (20 ml, lM) in 100 ml of dry diethyl ether under nitrogen atomosphere, a solution of t2S,3R)-3-[~(4-bromobenzyl)oxy]methyl~oxirane-2-methanol-1 (1.36 g, 5 mmol) in 140 ml of dry diethyl ether was added dropwise over 30 minutes. The mixture was vigorously stirred fo~
. . : ,. . . .
. . :.: : .. .
- : : , : ,: : ,: ;
::: . : : : :, , . ~ :',: . .. ~ :
WO92/06102 2 ~ 9 3 3 2 0 PCT/SE91/00653 30 minutes at -50C and then quenched with saturated aqueous ammonium chloride. The organic phase was collected and the aqueous phase was extracted with diethyl ether. The organic phases were combined and washed with hydrogen chloride (lM), sodium hydrogen carbonate (sat.), dri~d, filtered, concentrated and separated by column chromatography (toluene: ethyl acetate, 1:5), to give 2 (1 g, 64 %) ~and 3 (0.4 g, 25 %)], [~]22D: +1.56 (c 1.03, CHC13); lH-NMR (100 MHz, CDCl3): 1.8 (m lH, H-3); 2.13 (t, Jl~,2~ = Jl~,3 = 6-8 Hz, 2H, H-l'); 3-3 and 3.0 (broad, 2H, OH-2); 3.59 (m, 4H, H-1, H-4); 4.0 (m, lH, H-2); 4.48 (s, 2H, CH2Ph); 4.94 and 5.09 (m, 2H, H-3'a, H-3'b); 5.78 (m, lH, H-2');
7.14-7.5 (m, 4H, arom); 13C-NMR (25.05 MHz, CDC13); 30.6 (C-l');
42.3 (C-3); 63.3 (C-4); 71.9, 72.3, 72.5 (CH2Ph, C-1, C-2');
116.4 (C-3'); 121.5, 129.1, 131.3 (aromatic C); 136.4 (C-2 and aromatic C);
b~ (2S,3R)-4-O-Benzoyl-1-O-p-bromobenzyl-3-C-(2'-propenyl)-1,2,4-butan-triol (compound 4, scheme 1). Benzoyl chloride (3.21 ml, 27.6 mmol) was added dropwise to a solution of compound 2 (8.54 g, 27 mmol) in dry pyridine (50 ml) at 0C.
The reaction mixture was stirred at 0C for 15 minutes. Water (5 ml) was added and the solvent was evaporated. The residue was dissolved in dichloromethane and washed with hydrochloric acid (1 M), aqueous sodium hydrogen carbonate (sat.), dried, concentrated and purified by flash chromatography (toluene:
ethyl acetate, 2:1) to give compound 4 (9.77 g, 86 %). [~]22D:
+8.5 (c 0.71, CHCl3); lH-NMR (100 MHz, CDCl3): 1.95-2.43 (m, 3H, H-3, H-l'); 2.59 (d, JH(O~) ~ = 3-9 Hz, lH, OH-2); 3.54 (m, sec.
order, 2H, ~-la, H-lb); 3.95 (m, lH, H-2); 4.34 (d, J4 3 = 5.1 Hz, 2H, H-4); 4.48 (s, 2H, CH2Ph); 5.14 and ~.0 (m, 2H, H-3'a, H-3'b); 5.78 (m, lH, H-2'); 8.1-7.14 (m, 9H, ~rom.); 13C-NMR
(25.05 MHz, CDC13); 31.3 (C-l'; 40.4 (C-3); 70.1 C- 2; 64.0 (C-4); 7203 (CH2Ph); 72.5 (C-1); 116.3 (C-3'); 121.4-134.7 (arom.); 136.4 (C-2'); 166.1 (COPh).
c) Methyl 3-C-[(benzoyloxy) methyl]-5-O-p-bromobenzyl-2,3-dideoxy-D-erythro-pentofuranoside (compound 5, scheme 1). To an ice cold mixture of compound 4 (7.5 g, 17.9 mmol) and N-methylmorpholine-N-oxide (4.8 g, ~5.5 mmol) in ~ : :
..
.. ..
., .~ . . .
...
.
::
WO'~2/06102 2 0 9 3 9 2 U PCT/SE91/00653 --tetrahydrofuran:water (3:1, 70 ml), OsO4 in t-butanol (18 ml, 0.02 M, stab. with 1 % TBHP, 0.36 mmol) was added. After a few minutes, the ice bath was removed and the reaction mixture was stirred overnight at room temperature under nitrogen. NaHSO3 (2 g) was added and the mixture was stirred for 15 minutes. The solvent was evaporated off and the residue diluted with ethyl acetate, washed with HCl (1 M), NaHCO3 (sat.), dried, filtered and concentrated. The crude product was dissolved in tetrahydrofuran:water (3:1, 200 ml) and treated with NaIO4 (7.65 g, 35.8 mmol). The cis diol was completely claaved a~ter 30 minutes at room temperature. The tetrahydrofuran was evaporated off and the a~ueous residue was saturated with NaCl, and extracted with diethyl ether. The organic phase was dried and concentrated. The residue was treated with methanolic HCl (0.05 . . .
~, 50 ml) for ten minutes, neutralized with Dowex 2x8 (HCO3), filtered, evaporated and the residue was purified by flash chromatography (toluene-ethyl acetate, 3:1) to give an anomeric mixture of co~pound 5 (6.63 g, 85 %) as a colourless syrup.
lH-NMR (100 MHz, CDC13): 1.7-2.9 (three m, 3H, H-3, H-2a, H-2b);
3.31, 3.35 (2s, 3~1, OCH3); 3.6 (m, 2H, H-5); 4.1 (m, lH, H-4);
4.4 (m, 2H, H-6); 4.6 (m, 2H, CH2Ph); 5.1 (m, lH, H-1); 7.1-8~0 (m, 9H, aromatic); 13C- NMR (25.05 MHz, CDCl3): 35.6, 36.4 (C-2);
38.7, 39.3 (C-3); 54.3, 54.5 (OCH3); 65.7, 66.6, (C-6); 71.5, 72.35, 72.37, 73.8 (C-5) (CH2Ph); 79.9, 80.1 (C-4); 104.8 (C-1);
121.0- 136.4 (aromatic); 165.8 (COPh).
d) Methyl 5-O-benzoyl-3-C-[(benzoyloxy)methyl]-2,3,-dideoxy-D-erythro-pentafuranoside (compound 6, scheme 1). A
solution of compound 5 (1 g, 2,3 mmol) in dry diethyl ether (3 ml) was dissolved in liquid ammonia (50 ml) in a dewar bottle.
Sodium (300 mg, 13 mmol) was added in portions over 5 minutes.
The solution was stirred for 30 minutes and then quenched with solid NH4Cl. The ammonia was evaporated under a stream of nitrogen and the solid residue was diluted with ethyl acetate.
The solid was filtered off and washed several times with ethyl acetate. The filtrate was concentrated and then co-evaporated with dry toluene. The crude residue was dissolved in dry pyridine (30 ml). Benzoylchloride (0.8 ml, 6.9 mmol) was added and the solution was stirred for 40 minutes at room temperature :;
.. ,. . . . ,:
: . .:. . ..
: :. :: . , .
: . . ~
., ~
2~020 - , WO92/06102 PCT/SE91/~0653 after which water (5 ml) was added and the mixture was concentrated to dryness. The residue was dissolved in CH2Cl2 and washed with aqueous HCl (1 M), aqueous NaHC03 (sat.), dried, filtered and evaporated to dryness. Flash chromatography (toluene ethyal acetate, 3:1) yielded compound 6 (580 mg, 68 %), as an anomeric mixture. A small sample of compound 6 was separated by silica gel. lH-NMR and m.p. of the ~-anomer were in agreement with those previously reported.
The starting materials for the compounds in Exam~les 11-16 were prepared by the following sequence of reactions a-e:
a) Methyl 5-0-(p-bromobenzyl)-3-C-(hydroxymethyl)-2,3-dideoxy-D-erythro-pentofuranoside. Methyl 3-C-[(benzoyloxy]-5-0-(~-bromobenzyl)-2,3-dideoxy-D-erythro-pentofuranoside (1.61 mg, 3.70 mmol) was treated with methanolic ammonia (30 ml, saturated) for 24 h. The solvent was evaporated, and the residue was purified by flash column chromatography (toluene-ethyl acetate, 1:2) to give the title compound ~1.14 g, 93 %). Anal.
Calcd for C14H1904Br: 50.77; H, 5.78. F'ound: C, 50.67; H, 5.73.
b) Methyl 5-0-tp-bromobenzyl)-3-C-(fluoromethyl)-2,4-dideoxy-D-erythro-pentofùranside. To a cold solution (-15C) of the previous compound (637 mg, 1.92 mmol) in dichloromethane (15 ml) and pyridine (0.31 ml, 3.85 mmol) under nitrogen, a solutlon of trifluoromethanesulfonic anhydride (0.38 ml, 2.26 mmol) in dichloromethane (5 ml) was added dropwise. After stirring for lo min at -15C, the mixture was diluted with dichloromethane (100 ml), washed with l M hydrogen chloride, saturated aqueous sodium hydrogen carbonate, dried, and concentrated at a bath temperature not exceeding 20C. The residue was treated with a solution of anhydrous tetrabutylammonium fluoride in tetra hydrofuran (6 ml, lM) for 15 min. The mixture was concentrated and the residue was subjected to flash column chrom~tography (toluene-ethyl acetate, 9:1) to give the title compound (466 mg, 73 %). Anal. Calcd for C14H1803BrF: 50.46; H, 5.45. Found: c, 50.28; H.35.
,. ~ .
,.: ., ; . , : , WO~2/06102 2 Q 9 3 0 2 ;~ PCT/SE91/00653 c) Methyl 5-p-benzoyl-3-C-(fluoromethyl)-2,3-dideoxy-D-erythro-pentofuranoside. A mixture of the previous compound (572 mg, 1.72 mmol) in ethanol (40 ml) containing sodium hydrogen carbonate (excess) and 10 % Pd on charcoal (50 mg) was treated with hydrogen for 5 h at ambient pressure. The solids were filtered off and the filtrate was concentrated. The crude residue was dissolved in a mixture of dichloromethane (3 ml) and pyridine (0.3 ml, 3.7 mmol), followed by the addition of benzoyl chloride (0.24 ml, 2.07 mmol). The reaction mixture was stirred for 15 min. Water (2 ml) was added, and the mixture was stirred for 10 min, diluted with dichloromethane, washed with 1 M
hydrogen chloride, saturated aqueous hydrogen carbonate, dried, and concentrated. The residue was purified by flash column chromatography (toluene-ethyl acetate, 4:1) to give the title compound (372 mg, 81 ~). Anal. Calcd. for Cl~Hl7O4F: C, 62.68; H, 6.39. Found: C, 62.55; H, 6.33.
d) Methyl 3-C-(azidomethyl)-5-O--(p-bromobenzyl)-2,3-dideoxy-D-erythro-pentofuranoside.
To a mixture of methyl 5-O-(p-bromobenzyl-3-C-(hydroxymethyl)-2',3'-dideoxy-D-erythro-pentofuranoside (1.95 g, 5.89 mmol), triphenylphosphine (1.6 g, 6.1 mmol), and lithium azide (1.5 g, 30.6 mmol) in dry N,N-dimethylformamide (25 ml) was added carbon tetrabromide (1.95 g, 5.89 mmol) at room temperature. The mixture was vigorously stirred for 24 h.
~ethanol (3 ml) was added, and the solvent was evaporated. The mixture was diluted with dichloromethane, washed with water, dried, and concentrated. The residue was purified by flash column chromatography (toluene-ethyl acetate, 4:1) to give the title compound (1.99 g, 95 %) ~maX 2100 cm-1. Anal. Calcd. for C14~l8O3BrN3: C, 47.20; H, 5.09; N, 11.80. Found;C, 47.15; H, 4.98; N, 11.9.
e) Methyl 3-C-(azidomethyl)-5-O-(benzoyl)-2,3-dideoxy-D-erythro-pentofuranoside. To a stirred solution of dry pyridine (4.7 ml, 58.3 mmol) and dichloromethane (20 ml) was added chromium trioxide (2.8 g, 28.0 mmol). The mixture was stirred for 15 min at room temperature. A solution of the previous compound (1.68 g, 4.72 mmol) in dichloromethane (20 ml) was : . .
: . ~ .~ . . .
: :.: ~
W O 92/06102 2 Q ~ 3 0 2 a P ~ /SE91/00653 added, followed by acetic anhydride (3.2 ml, 32.0 mmol), and the mixture was stirred for 10 min at room temperature. The mixture was passed through a short column of silica gel with ethyl acetate as eluent, to give a crude product, which was treated with methanolic ammonia (30 ml, saturated) for 24 h. The solvent was evaporated, and the residue was purified by flash column chromatography (toluene-ethyl acetate, 1:1) and then dissolved in a mixture of dichloromethane (15 ml) and pyridine (1 ml, 12.4 mmol), after which benzoyl chloride (0.55 ml, 4.73 mmol) was added. The reaction mixture was stirred for 15 min.
Water (2 ml) was added, and the mixture was stirred for 10 min, diluted with dichloromethane, washed with 1 M hydrogen chloride, saturated aqueous hydrogen carbonate, dried, and concentrated.
The residue was purified by flash column chromatography (toluene-ethyl acetate, 9:1) to give the title compound (1.0 g 57 %). Anal. Calcd for C14H17O4N3: C, 57.72; H, 5.88; N, 1~.43.
Found: C, 57.86; H, 5.74; N, 14.43.
The starting material for the compounds in Example_17 were prepared by the following sequence of reactions a b:
a) (3S,4S~-3,4-di(benzoyloxymethyl)cyclopentanone (compound 8, Scheme 2). A mixture of (-)(3S,4S)-3,4-di(methoxycarbonyl)-cyclopentanone (compound 7, Scheme 2) (0.93 g, 4.6 mmol), ethylene glycol ~6,5 ml, 0.12 mol) and p-toluenesulfonic acid monohydrate (25 mg) in toluene (50 ml) was refluxed for 6 h with a Dean-Stark trap. Sodium hydrogen carbonate (20 mg) was added and after stirring for 5 min the mixture was washed with saturated aqueous sodium hydrogen carbonate, dried (MgS04) and concentrated to a cr-lde ketal diester. According to lH NMR, the product was free from unreacted ketone. The crude ketal diester in dry diethyl ether (15 ml) was added dropwise for 1 h to an ice-cold mixture of lithium aluminium hydride ~0~35 g, 9.2 mmol) in dry diethyl ether (35 ml). The mixture was stirred at room temperature for 3 h before the excess of lithium aluminium hydride was decomposed by successive addition of water (0.5 ml), 3 M aqueous NaOH (0.5 ml) and water (1.5 ml). After stirring for 1 h MgSO4 (20 g) was added and the stirring was prolonged for 5 min. The precipitate and MgSO4 were remov~d by filtration and washed several times .
.
WO92/06102 2 ~ ~ 3 0 2 ~ 38 PCT/SE91/00653 ~
with ethyl acetate. The filtrate was concentrated to give a crude oil of the diol. To a stirred solution of the diol in pyridine (13.5 ml) was added benzoyl chloride (2.3 ml, 2001 mmol) dropwise. The mixture was stirred for 3 h in room temperature. Water (5 ml) was added and after stirring for 10 min, the mixture was diluted with saturated aqueous sodium hydrogen carbonate and dichloromethàne. The organic layer was separated and washed with saturated sodium hydrogen carbonate, dried (MgSO4) and concentrated. Column chromatography (toluene-EtOAc, 3:1) on a short column of silica gel gave a syrupy residue including the dibenzoylated Xetal and traces of the dibenzoylated ketone. The syrup was dissolved in methanol (9~
ml) and 2 M aqueous HCl (31 ml), stirred at room temperature for 2 h and then at 50C for additional 2 h. The solution was neutralized with sodium hydrogen carbonate (5.2 g), diluted with water, extracted with dichloromethane. The organic layer was dried (MgSO4), concentrated and crystallized from diethyl ether/hexane to give the title compound as needles (0.90 g, 55%): m.p. 85-86C; [~]D-59.1 (c 1.04, chloroform);; lH NMR (100 MHz, CDC13) ~ 2.12-2.73 (m, 6H, 2 CH, 2 CH2), 4.50 (d, 4H, 2 CH2-OBz), 7.36-7.64 and 7.94-8.07 (m, 10H, aromatic H); 13C NMR
(25.05 MHz, CDC13) ~ 38.3 (2 CH2), 41.6 (2 CH) 65.9 (2 CH2-OBz), 128.2, 128.2 and 133.0 (aromatic C), 165.9 and 214.9 (C=O).
Anal. Calcd for C21H20O5 (352.4): C, 71.58; H, 5.72. Found: C, 71.34; H, 5.73.
b) (3s,4S)-3,4-di(benzoyloxymethyl)cyclopentanol (compound 9, Scheme 2). To the compound 8 obtained under a) (193 mg, 0,55 mmol) in methanol (10 ml) was added sodium borohydride (41 mg, 1,10 mmol~ in successive portions. The mixture was stirred at room temperature for 30 min, and then diluted with water and diethyl ether. The organic layer was washed with saturated agueous sodium chloride r dried (MgSO4) and concentrated to a syrup which was purified by column chromatography on silica gel (toluen/EtOAc, 1 1) to yield title compound (189 mg, 97%; [~]D
_16.4 [c 1.09, chloroform); 1H NMR (100 NHz, CDC13) ~ 1.6-2.7 (m, 7H, 2 CH, 2 CH2, OH), 4.41 ~t, 4H, 2 CH2-OBz), 7.34-7.56 and 7.97-8.09 (m, 10H, aromatic H); 13C NMR (25.05 MHz, CDC13) ~
38.3, 39.3, 39.8, 40.0 (2 CH2 2 CH), 67.4, 68.2 (2 CH2-OBz), 72.5 - .. .. ., ~.,.,.. ~ , .
.: ~ :, . - .:
,~
WO ~2/06102 ~5i3 ~ 3 o 2 ~ PCI/SE91/û0653 (CHOH), 128.1, 129.3, 129.8, 132.8 ~aromatic C), 166.3 tC=O)-Anal. Calcd for C2lH22O5 (354.4): C, 71.17; H, 6.26. Found: C, 71.05; H, 6.18.
The starting materials for the compounds in Examples 18 - 22 were prepared by the following sequence of reactions a-c:
a) (S)-4-tert-Butyldiphenylsilyloxy)methyl-(R,S)-2-hydroxymethyl-~-buty-- ~lactone (11). Ethanol (28 ~Ll, 0.48 mmol) was added to sodium (~80 mg, 12.17 mmol) in dry diethyl ethe~ (5 ml), and the mixture was stirred at room temperature for 2 h. A
solution of (S)-4-(tert-butyldiphenylsilyloxy)methyl-~-butyrolactone (3.50 g, 9.87 nunol) and ethyl forma~e (980 mg, 13.23 mmol) in diethyl ether (6 ml) was added and the stirring was continued for 16 h at room temperature. Diethyl ether (20 ml) and 1 M NaH2PO4 (10 ml) were added and the phases were separated. The organic phase was washed with water, dried and concentrated. ~he residue was dissolved in ethanol tl0 ml), and a solution of NaBH4 (500 mg, 13.22 mmol) in ethanol (10 ml) was added. After 5 min, the reaction was 1:erminated by the addition of a few drops of 80 % acetic acid. The mixture was partitioned between ethyl acetate and water. The organic phase was dried, concentrated and the residue purified by column chromatography (toluene-acetone 9:1) yielding 11 (3.40 g, 90 %).
Anal. Calcd for C22H2~o4Si: C, 68.71; H, 7.34. Found:
C, 68.58; H, 7.36.
b) (S)-2-Acetoxymethyl-(S)-4~(tl~rt-butyldiphenyl-silyloxy)methyl-~-butyrolactone( 12) . A solution of 11 (2.00 ~, 5.20 mmol) in pyri.dine (20 ml) and acetic anhydride (~0 ml) was stirred at 60C for 30 min. The solut on was concentrated and the residue purified by column chromatography (toluene-acetone 1) to give 12 (1.41 g, 64 %-): [~]D +22.4 (c: 1.00, CHCl3): 13c ~,X (CDCl3) ô 29.3 (C, tert); 20.8 (CH3, acetate), 26.9 (3 x CH3), 27.3 (C-3), 39.8 ~C-2~, 63.1, 65.7 (CH20Ac, C-5), 78.1 (C-4), 127.9, 128.3-135.7 (ArC), 170.7 (acetate), 176.4 (carbonyl~;
lH NMR (CDCl3) ~ 1.05 (s, 9H, 3 x CH3), 2.07 (s, 3H, CH3, acetate), 2.39 (m, 2H, H-3, H 3') j 3.13 (m, lH, H-2), 3.79, 4.35 (m, m, 4H, H-5, H-5', H-6, H-6'), 4.58 (m, lH, H-4), 7.17-7.72 ... .. .
. .
, , -': . ~ '` ., ': ' WO92/06102 2 0 9 3 0 2 ~ PCT/SE91/006~3 -(m, lOH, ArH).
Anal. Calcd. for C24H30o5si: C, 67-57; H, 7-09- Found:
C, 67.48; ~, 7.15.
c) 2-C-Acetoxymethyl-l-O-acetyl-5-O-(tert-butyldiphenyl)-silyl-D-erythro-pentofuranose (13). To a solution of lactone 12 (500 mg, 1.17 mmol) in toluene (30 ml) at ~78C was added diisobutylaluminium hydride (20 ~ in hexane, 3.00 ml, 2.95 mmol). The solution was stirred for 1.5 h, allowed to warm to room temperature, and methanol (0.5 ml) was added. To the solution was added ethyl acetate (30 ml) and aqueous sat. NaHC03 (4 ml). After stirring for 2 h, dry MgS04 (2.0 g) was added and after additional stirring for 3 h, the mixture was filtered. The filtrate was concentratPd, the residue dissolved in pyridine (6 .
ml) and acetic anhydride (3 ml) and heated to 40C for 30 min, concentrated and the residue purified by column chromatosraphy (toluene-acetone 10:1) to yield the title compound (500 mg, 91 %) as an anomeric mixture. 13C NMR (CDCl3, selected signals) 19.4 (C, tert.), 20.9, 21.3, 21.4 (CH3, acetates), 26.9 (3 x CH3), 28.4 (C-3), 42.3, 44.8 (C-2), 62.8, 63.7, 65.8, 66.8 (C-5 and C-6), 80.0, 81.4 (C-4), 97.6, 100.2 (C-1), 127.8-135.7 (ArC), 170.2, 170.9 (acetates).
Anal. Calcd. for C26H3406Si: C, 66.35; H, 7.29. Found:
C, 66.51; ~, 7.28.
The starting materials for the compounds in Examples 23 - 24 were prepared by the following sequence of reactions a-c:
a) 5-0-Benzoyl-3-C~[(benzoyloxy)methyl]-2,3-dideoxy-L-threo-pentose dibenzyl dithioacetal (lS).
Compound 14 (0.191 g, 0.516 mmol) was treated at room temperature with benzyl mercaptane (0.24 ml, 2.07 mmol) in dichloromethane (3 ml) containing a catalytic amount of stannic chloride for 24 hours. ThP mixture was then diluted with dichloromethane and washed with saturated aqueous sodium hydrogen sulfate, dried, filtered, concentrated and purified by flash column chromatography (toluene-ethyl acetate 9:1) to give compound 15 (0.266 g, 88 ~) as a colourless syrup: [~]22D-8.1 (c 1.0, CHCl3). 13C NMR (25.05 MHz, CDCl3) ~ 34.0, 34.6, 34.9 (C-2, ; . ~:: . : :. ;,, . , :
-. . : : :; , .. :
W092/06102 2 ~ ~ 3 Q 2 a PCT/SE91/00653 ~1 CH2Ph), 38.9 (C-3), 47.9 (C-1), 63.2 (C-4), 67.6 (C-5), 69.8 (c-6), 127.1-138.0 (aromatic), 166.7 (COPh).
b) Benzyl 5-O-benzoyl-3-C-[(benzoyloxy)methyl?-2,3-dideoxy-1,4-dithio-a-and-B-D-erythro-pentofuranoside (16). To a solution of compound 1~ (0.347 g, 0.592 mmol) in tolu ne-acetonitrile (2:1, 9 ml) were added triphenylphosphine (0.93 g, 3.55 mmol) and triiodoimidazole (0.79 g, 1.78 mmol). The mixture was kept at 100C over night. The mixture was diluted with toluene and washed with saturated aqueous sodium hydrogen sulfate, dried, filtered, concentrated and purified by column chromatography (toluene-ethyl acetate 50:1) to give 16 (0.219 g, 77 %) as a colourless syrup: [~22D-4.2 (c 1.0, CHCl3); 1H NMR
(100 MHz, CDCl3) ~ 2.0-2.3 (m, 2H, H-2), 2.8 (m, lH, H-3), 3.8 (m, 3H, CH2Ph, H-4~, 4.5 (m, 5H, H-1, H-5, H-6), 7.1-8.1 (m, 15H, aromatic).
c) Acetyl 5-O-benzoyl-3-C-[(benzoyloxy)methyl]-2,3-dideoxy-4-thio-~-and-~-D-erythro-pentofuranoside tl7). Compound 16 (0.182 g, 0.381 mmol) and mercuric acetate (0.243 g, 0.761 mmol) in glacial acetic acid (5 ml) were stirred at room temperature for 30 min. The solvent was evaporated and co~
evaporated with dry toluene. The residue was diluted with dichloromethane, filtered through celite and concentrated. Flash chromatography (toluene-ethyl acetate 9:1) gave compound 17 (0.156 g, 99 -~ as a colourless syrup: lH NMR (100 MHz, CDCl3) ~
2.03, 2.07 (2s, 3H, COOCH3), 2.3 (2H, H-2), 3.0 (m, 1~, H-3), 3.8 (m, lH, H-4), 4.4 (m, 4H, H-5, H-6), 6.2 (m, lH, H--1), 7.0-7.9 (m, 10H, aromatic). ` `
Test I. Effect of compounds of the formula I on HIV in H9 cells HIV infection_of H9 cells H9 cells, 105 cells per well on a 24 well plate, suspended in 2 ml RPMI-medium containing 10 % fetal calf serum, 100 mg/ml penicillin, 10 mg/ml streptomycin sulfate and 2 mg/ml polybrene ar~ exposed to HIV (HTLV-IIIB) and different , .; , . . : ..... :
. .
. , .: :
, -W O 92/06102 2 0 9 3 0 2 ~ P ~ /SE91/00653 concentrations of the test compounds. The plates are incubatedat 37C in 5 % C02 for 6-7 days. The contents in each well is then homogenized with a pipette and transferred to a centrifuge tube. After centrifugation for 10 min at 1500 rpm the supernatant is removed and the cell pellet is analyzed by fixing in methanol on glass plates. Human HIV positive serum diluted 1:80 or 1:160 is added and incubated for 30 min at 37C. The plate is then washed with phosphate-buffered saline (P8S) containing Ca2+ and Mg2+. Sheep antihuman conjugate (FITC) is added and after a new incubation the plate is again washed with PBS. Contrast staining is done with Evans blue and after drying the frequency of HIV antigen containing cells is determined in a microscope. The test result is shown in Table 1.
Table 1 Concentration (m~) for 50 % i~hibition ~ICso~ of human immuno deficiency virus multiplication in cell culture _________________________________________________________~______ Compound IC50 ~M
________________ _______________________________________________ 1-[2',3'-Dideoxy-3'-C-(hydroxymethyl)-~-D--erythro-pentafuranosyl]cytosine 0.01 1-[2',3'-Dideoxy-3'-C-(hydroxymethyl)-~-D- ' -erythro-pentafuranosyl]cytosine 5 1-[2',3'-Dideoxy-3'-C-(hydroxymethyl) ~-D--erythro-pentAfuranosyl]thymine 5 1-[2',3'-Dideoxy-3'-C-(hydroxymethyl)-~-D--erythro-pentafuranosyl]thymine 10 r 9-[2',3'-Dideoxy-3'-C-(hydroxymethyl)~-D--erythro-pentafuranosyl]adenine 10 _____..__________________________________________________________ Table 1 shows that the tested compounds are active inhibitors of HIV virus multiplication.
Test II. Cellular toxicity ,'' ', ~' , ; , ' ~ ' " ,:
:: . :
W O 92/06102 2 ~ ~ 3 O 2 ~ PC~r/SE91/006~3 H9 cells, 2x107 cells per plate, are incubated in RPMI-1640 medium containing 10 % fetal calf serum, 70 mg/l penicillin, 100 mg/l streptomycin and 10 mM hepes, in absence or presence of test compounds. The number of cells per plate is determined after 48 h. Cells incubated in the absence of test compounds then underwent two cell division cycles.
F5000 cells, Jhich are human embryo cells, lx105 cells per plate, are incubated in Eagle's minimal essential medium, supplemented with Earle's sals, non-essential amino ac,ds, 10 %
fetal calf serum, 10 mM hepes, 70 mg/l penicillin and 100 mg/l streptomycin, in absence or presence of test compounds. The number of cells per plate is determined after 48 h. Ce incubated in the absence of test compounds underwent c . -ell division cycle. The results are given as % inhibition . cell multiplication and are given in Table 2.
Table 2. Cellular toxicity on H9 ~lls __________________________________________________________~_____ Compound % Inhibition (H9) .____________________________________.__________________________ 1-[2',3'-Dideoxy-3'-(hydroxymethyl)-~--D--erythro-pentofuranosyl]cytosine (1 mM) 50 _____________~_______________________.__________________________ Table 2 shows that the concentration at which the compound exhibit toxicity exceed the concentrat ~n needed for 50 ~ inhibition of HIv multiplication as given in Table 1.
. , . ~ ,: , ~ , . .
, : . . : . . . .: , : .
::: : : . .
:: ~ : ;. :. ::
-, , ;
WO92/06102 2 ~ 3 3 ~ 2 ~ PCT/SE91/00653 9-[2',3'-Dideoxy-3'-C (fluoromethyl)-~-and-B-D-erythro-pentofuranosyl]adenine. 6-Chloropurine (120 mg, 0.78 mmol) was silylated according to the general procedure and the synthesis was proceeded as described above to give the title compounds ~
(25 mg, 26 %) and B (37 mg, 39 %). a: [~22D 35.2 (c 0.71, H20);
W (H2O) ~maX 260 nm (~ 10239); 1H NMR (270 MHz, D20) ~ 2.5 (m, H-2'a), 2.70-2.95 (m, H-Z'b and H-3'), 3.73 (dd, H-5'a), 3.38 (dd, H 5'b) 4 40 (m H-4), 4.52-4.78 (2 m, J3~,6~b = 10-0 Hz, H
b), 6.34 (t, H-l'), 8.15 (s, H-2), 8.29 (s, H-8). Anal. Calcd.
for C~1H14O2NsF: C, 49.43; H, 5.28. Found: C; H. B: [~)22D -24.3 (c 0.9, H20); W (H20) ~maX 260 nm (~ 10364); lH NMR (270 MHz, D20) ~ 2.47-2.48 (2 m, H-2'a,b), 2.85 (m, H-3'), 3.69 (dd, H-5'a), 3.87 (dd, H-5'b), 4.21 (m, H-4), 4.51-4.78 (2 m, H-6'a,b), 6.24 (dd, H-l'), 8.04 (s, H-2), 8.25 (s, H-8). Anal. Calcd. for C11H1402N5F: C, ~9-43; H, 5.28. Found: C,; H,.
1-[2',3'-Dideoxy-3'-C-(azidornethyl)-~-and-B-D-erythro-pentofuranosyl]-thymine.
Thymine (133 m~ 1.06 mmol) was silylated according to the general procedure an dissolved in dichloromethane (5 ml) under nitrogen. To this solution was added methyl 3-C
(azidomethyl)-5-O-(benzoyl)-2',3'-dideoxy-D-erythro-pentofuranoside (154 mg, 0.53 mmol) followed by tert-butyldimethylsilyl triflate (0.25 ml, 1.09 mmol). The solution was stirred for 24 hours at room temperature, after which the reaction was quenched by the addition of aqueous sodium hydrogen carbonate, stirred for 30 min, diluted with dichloromethane, washed with aqueous sodium hydrogen carbonate, dried, and concentrated to give an anomeric mixture of the protected nucl~osides. The mixture was treated with mPthanolic ammonia (20 ml, saturated) for 24 h at room temperature. After concentration to dryness, the residue was dissolved in water and washed with dichlorome~hane. The aqueous layer was concentrated to a small volume and freeze dried to give 110 mg (0.39 mmol, 74 %) of an anomeric mixture of the deblocked nucleosides. The mixture was dissolved in a mixture of N,N-dimethylformamid and imidazole (53 mg, 0.78 mmol) followed by the addition of tert-butyldimethyl-. .
~-': . :
WO92/06102 ~93 ~ 2 ~ PCT/SE91/00653 silyl chloride (70 mg, 0.46 mmol), and the solution was stirred for 3 h at room temperature. Water (1 ml) was added, and the mixture was stirred for 10 min, diluted with dichloromethane, washed with 1 M hydrogen chloride, saturated aqueous hydrogen carbonate, dried, and concentrated. The anomers were separated by column chromatography (hexane-ethyl acetate 1:1), the ~-anomer was eluted first followed by the B-anomer. Each of the anomers was separately treated with tetrabutyl-ammonium fluoride (70 mg, 0.22 mmol) in tetrahydrofuran (2 ml) for 24 h, concentrated, the residue dissolved in water, and washed with dichloromethane. The aqueous layer was concentrated to a small volume and purified bv HPLC (water-methanol, 80:20, v/v) to give the title compounds ~ (43 mg, 29 %) and ~ (44 mg, 30 %). ~:
[~]22D +3.1 (c 1.45, H2O); W (H2O) ~max 268 nm (~ 9155); lH NMR
(270 MHz, D2O) ~ 1.92 (s, 5-CH3), 2.02 (m, H-2'a), 2.50-2.74 (2 m, H-2'b and H-3'), 3.54 and 3.58 (2 dd, H-6'a,b), 3.67 tdd, H-5'a), 3.85 (dd, H-5'b), 4.25 (m, J4~ 5~a = 5.1 Hz, H-4), 6.12 (dd, H-1), 7.60 (d, H-6). Anal. Calcd. for Cl1Hl504N2F: C, 51.16;
H, 5.85. Found: C.; H,. ~ [~]22D 12.5 (c 0.96, H2O~; W (H2O) ~max 268 nm (~ 9434); lH NMR (270 MHz, D20) ~ 1.91 (s, 5-CH3), 2.34 (dd, H-2'), 2.59 (m, H-3'), 3.47-3.61 (2 dd, H-6'a,b), 3.78 (dd, H-5'a), 3.94 (dd, H-5'b), 3.98 (m, H-4), 6.1 (t, H-1), 7.7G
(s, lH, H-6). Anal. Calcd. for CllHl504N5: C, 46.97; H, 5.38.
Found: C,; H,.
1-[2',3'-Dideoxy-3'-C-(azidomethyl)-~-and-B-D-erythro-pentofuranosyl]cytosine.
Cytosine (250 mg, 2.25 mmol) was silylated according to the general procedure and the synthesis was proceeded as aboYe to give the title compounds ~ (84 mg, 43 ~) and B (45 mg, 23 %).
~ [~22D -46.1 (c 1.15~ H20); W (H2O) ~max 27~ nm (~ 8320); H
N~R (270 MHz, D20) ~ 1.96 (m, H-2'a), 2.56 (m, H-3'), 2.74 (m, H-2'b), 3.46-3.58 (2 dd, H-6'a,b), 3.68 (dd, H-5'a), 3.85 (dd, H~
5'b), 4.25 (m, H-5), 6.09 (t, H-1), 7.79 (d, H-6). Anal. Calcd.
for CloH1403N6: C, 45.10; H, 5.30; N, 31.57. Found: C, 4~.~7; H, 5.13; N, 31.37. ~ [~]22D 55.00 (c o.99, H20); W (H2O) ~m~x 272 nm (~ 8209); 1H NMR (270 MHz, D2O) ~ 2.22-2.58 (m, H-2' and H-3'), 3.48-3.61 (2 dd, H-6'), 3.79 (dd, H-5'a), 3.94 (dd, H-5'b), 4.0 ..... .
'. . ' : ., :
~3023~
WO92/06102 PCT/S~91/00653 (m, H-4'), 6.04 (d, H-~), 6.11 (dd, H-1'), 7.93 (d, H-6). Anal.
Calcd- for C1oHl4O3N6: _, 45-10; H, 5.30. Found: C,; H .
9-~2',3'-Dideoxy-3'-C-(azidomethyl)-~ and B-D-erythro-pento~uranosyl]-adenine.
6-chloropurine (360 mg, 2.33 mmol) was silylated according to the general procedure and the synthesis was proceeded as above to give the title compounds ~ (38 mg, 17 ~) and B (39 mg, 17 %). An analytical sample of each of the anomers was obtained by HPLC purification (water-methanol, 70:30, v/v).
~: [a]22D +58.3 (c 1.0, H20); W (~2) ~max 260 nm (~ 9353); 1H
~NR 270 MHz, D20) ~ 2.43 (m, H-2'a), 2.67 (m, H-3'), 2.8~
(m,H-2'b), 3.61 (d, H-6'), 3.73 (dd, H-5'a), 3.88 (dd,h-5'a), 4.28 (m, H-4'), 6.28 (t, H-1'), 8.11 (s, H-2), 8.28 (s, H-8).
Anal. Calcd for C11Hl4O2N8: C, 45.51; Gm 4.86; N, 38.61. Found:
C, 45.31; Gm 4.80; N, 38.30. B. [~22D -27.6 (c 1.05, H2O; W
(H2O) ~max - nm (~ 14102); 1H NMR 270 MHz, D2O) ~ 2.52 (m, H-2'a), 2.75 (m, H-2'b, H-3'), 3.61 ~dd, H-6'a), 3.64 (dd, J3 6~b = 6.2 Hz, H-6'b), 3.?1 (dd, H-5'a, 3.88 (dd, H-5'b), 4.12 (m, H-4'), 6.36 (dd, and 3.0 Hz, H-1'), 8.19 (s, H-2), 8.33 (s, H-8). Anal. Calcd for CllH14O2N8: C, 45.51; H, 4.86; N, 38.61.
Found: C, 45.28; H, 4.97; N, 38.36.
Example 17 9-[3S,4S)-3,4-di(hydroxymethyl)cyclopentyl~-guanosine To a white suspension of PPh3 (528 mg, 2.0 mmol) and 2-NH2-6-Cl-purin (342 mg, 2.0 mmol in dry THF (14 ml) at room temperature under nitrogen, was added DEAD (Diethylazodicarboxylate) (0.33 ml, 2.3 mmol) and the yellowish suspension was stirred for an additional 4 h. 3,4-dibenzoyloxymethyl-cyclopentanol (200 mg, 0.57 mmol) in dry THF (5 ml) was added and the mixture was stirred for an additional 30 min. The solvent was evaporated and the crude residue was purified by silica gel column chromatography (toluene-ethyl acetate, l:l). The crude product was dissolved in MeOH (2 ml), aqeous NaOH(lM, 2ml) was added dropwise, and the mixture was stirred at 80C for 4 h. After cooling, the reaction mixture was ~ :; . .
-WO92/06102 2 Q 9 3 0 2 ~ PCT/SE9l/00653 -neutralized with aqeous HCl (2M), the aqeous phase was extracted several times with THF-Ethylacetate (1:1) and the solvents were evaporated. The crude product was purified by silica gel column chromatography (chloroform-methanol, 6:1). Recrystallisation from water afforded the title compound in a yield of 57 g (36%).
Exam~le 18 1-(2',3'-Dideoxy-2'-C-hydroxymethyl-~-D-erythro-pentofuranosyl)-thymine A mixture of thymine (253 mg, 2.01 mmol), chlorotrimethylsilane (0.300 ml) and some crystals of (NH4)2SO4 in hexamethyldisilazane (4 ml) was refluxed under nitrogen for 6 h. The clear solution was concentrated and residual volatiles were co-evaporated with added toluene (5 ml). The residue was dissolved in a mixture of dichloromethane-acetonitrile (9:1, 10 ml) and a solution of 2-C-acetoxymethyl-l-O-acetyl-5-O-(tert-b~ltyldiphenyl)silyl-D-erythro-pentofuranose (630 mg, 1.34 mmol) in the same solvent mixture as above (5 ml) was added. The solution was cooled on an ice bath an~ tert-butyldimethylsilyl triflate (0.370 ml, 1.16 mmol) was added dropwise. The mixture wc~s stirred for 30 min on an ice bath followed by 15 h at room temperature. Pyridine (2 ml) was adde~3 and the mixture was iltered through a pad of silica gel. The filtrate was concentrated and the residue purified by column chromatography (toluene-acetone 1:1) to yield 1-(2'-C-acetoxymethyl-5'-O-tert-butyldiphenylsilyl-2',3'-dideoxy-B-D-erytro-pentofuranosyl)-thymine. 100 mg, 0.186 mmol of this material was dissolved in 1 M tetrabutylammonium fluoride x 3 H2O in THF (9 ml) and stirred for 20 min at room temperature. The solution was concentrated and the residue purified by column chromatography (toluene-acetone 1:1). The product was dissolved in methanol (4 ml) and methanol saturated with ammonia (2 ml) was added. The solution was stirred over night, concentrated and the residue purified by column chromatography (chloroform-methanol 9:1) yielding the title compound (38 mg): ~]D +9.1 (c 0.70, H?O): 13C NMR (D2O, 40C) 12.3 (CH3, thymine), 29.2 (C-3'), 46.7 (C-2'), 62.4, 63.8 (C-5', C-6'), ~1.0 (C-4'), 88.8 (C-l'), 112.2 (C-5, 138.6 (C-6), 152.5 (C~4), 167.2); lH NMR (D2O, 40C) 1.90 (s, 3H, CH3, thymine), 2.09 (m, 2H, H-3', H-3''), 2.64 (m, lH, H-2'),3.76 (m, , WO~/0~102 209302a PCT/SE91/006~3 4H, H-5', H-5'', H-6', H-6''), 4031 (m, H-4'), 5.93 (d, J=5.13 Hz, lH, H-1'), 7.68 (s, lH, H-6).
Anal. Calcd. for Cl1H1605N2o C, 51.56; H, 6.29; N, 10.
93. Found: C, 51.28; H, 6.11; N, 10.71.
1-(2',3'-Dideoxy-2/-C-hydroxymethyl-B-D-erythro-pentofuranosyl)cytosine was synthesizied analogous to Example 12 ;
using cytosine instead of thymine yielding (41 mg): [~]D + 31.2 tc 1.20, H20) 13C NMR (D20, 40C) 29.2 (C-3'), 47.6 (C-2'), 62.5, 64.0 (C-5', C-6'), 81.2 (C-4'), 89.8 (C-l'), 96.8 (C-5), 142.6 (C-6). 158.3 (C-4), 166.9 (C-2); lH NMR (D2O, 40C) 2.04 (m, 2H, H-3', H-3''), 2.55 (m, lH, H-2'), 3.77 (m, 4H, H-5', H-5'', H-6', H-6''), 4.33 (m, lH, H-4'), 5.91 (d, J=4.77 Hz, lH, H-1'), 6.05 (d, J= 7.32 ~z, lH, H-5), 7.85 (d, J= 7.32 ~z, lH, H-6)-Anal. Calcd. for CloHl504N3 x H20: C, 46.33; H, 6.61; N, 16.21. Found: C, 46.41; H, 6.31; N, 16.16.
2',3'-Dideoxy-2'-C-hydroxymethyladenosirle was synthesized analogous to Example 12 u~;ing adenine instead of thymine yielding (20 mg): ~]D -10.3 (C 0.20, H2O): W (H20~
~max ~60 nm; 13C NMR (D20, 40C) 29.4 ~C-3'), 46.8 (C-2'), 62.2, (C-5', C-6'), 81.4 (C-4'), 88.3 (C-1'), 141.2-169-4 (5ArC); lH
NMR (D20, 40C) 2.21 (m, 2H, H-3', H-3''~, 3.06 (m, lH, H-2'), 3.76 (m, 4H, H-5'', H-6', H-6''), 4.45 (m, lH, H-4'), 6.07 (d, J= 5.32 Hz, lH, H-1'), 8.15, 8.30 (s, s, 2H, H-2, H-8).
Anal. Calcd. for C11H1503N5: C, 49081; ~, 5.70; N, 26.40. Found: C, 50.01; H, 5.54; N, 26.35.
1-(2'-C-Azidomethyl-2',3'-dideoxy-~-D-erytro-pentofuranosyl)-thymine.
A solution 1-(2'-C-acetoxymethyl-5'-0-tert-butyldiphenyl-2',4'-dideoxy-~ D-erytro-pentofuransyl)thymine (440 mg, 0.820 mmol) in methanol saturated with ammonia (15 ml) was stirred over night. The mixture was concentrated and the residue purified by column chromatography (toluene-acetone 1 '" . '` . ' ' ' ,..
: . .: . : . :.
:,, ., :, ~,;~ ' , ': '' ~
WO92/06l02 2 Q ~ 3 0 ~ ~ PCT/SE91/00653 --The residue (170 mg, 344 mmol) was dissolved in pyridine (5 ml) and methanesulfonyl chloride (0.030 ml, 379 mmol) was added.
After 1 h at room temperature, the solution was concentrated and the residue co-evaporated with added toluene. The residue was purified by column chromatogrphy (toluene-acetone 2:1) yielding 1-(5'-O-tert-butyldiphenylsilyl-2'-C-methanesulfonylmethyl-2',3'-dideoxy-~-D-erythro-pentofuranosyl)thymine which was dissolved in DMF (2 ml). Sodium azide (72 mg, 1.11 mmol) was added and the resulting suspension was stirred at 60C for 3 h.
The mixture was concentrated and purified by column chromatography (toluene-acetone 2:1) (15~ mg) (IR(CHCl3) 2100 cm~
1), which was dissolved in 1 M tetrabutylammonium fluoride x 3 H2O in THF (10 ml) at room temperature. After 10 min, the mixture was concentrated and the residue purified by column chromatography (tsluene-acetone 1:1) yielding the title compound (72 mg). ~] D -31.3 (c 0.76, CHCl3): IR (CHCl3) 2100 cm-1 (azide): 13C NMR (CDCl3) 12.5 (CH3, thymine), 29.6 (C-3'), 44.5 (C-3), 52.0 (C-6'), 63.8 (C-5'), 79.8 (C-4'), 89.0 (C-1'), 111.0 (C-5). 136.6 (C-6), 150.9 (C-4), 164.4 (C-2); 1H NMR (CDCl3) 1.89 (s, 3H, CH3, thymine), 1.96 (m, lH, H-3'), 2.28 (m, lH, H-3''), 2.51 (m, lH, H-2'), 3.53, 3.82 (m, m, 4H, H-5', H-5'', H-6', H-6''), 4.29 (m, Ih, H-4') S.81 (cl, J=5.50 Hz, lH, Hl', 7.~6 (s, lH, H-6), 9.90 (s, lH, H-3).
Anal. Calcd. for C11H1504Ns: C, 46.97; H, 5.38; N, 24.90. Found: C, 46.83; H, 5.21; N, 2~L.71.
2'-C-Azidomethyl-2',3'-dideoxycytidine A solution of 2'-C-acetoxymethyl-5'-O-tert-butyldiphenyl-2',3'-dideoxy-cytidine (260 mg, 0.489 mmol) in methanol saturated with ammonia (10 ml) was stirred over night at room temperature. The mixture was concentrated and the residue purified by column chromatography (chloroform-methanol 9:1) to give 395 mg of the de-o-acetylated cytidine analogue. To a stirred solution of this material (100 mg, 0.208 mmol) in pyridine (3 ml) was added methanesulfonyl chloride (0.020 ml, 0.250 mmol). After 1 h the solution was concentrated and the residue was coevaporated with added toluene. The residue was purified by column chromatography (chloroform-methanol 9:1) (110 -:
: : .
wo 92/06102 2 0 ~ 3 0 2 ~ PCT/SE91/00653 mg) 13C N~R (CDCl3~ 37.3 (CH3), 69.7 (C-6'); 1H NMR (CDCl3) 3.08 (s, 3H, CH3), 4.50 (d, J=4.21 Hz, 2H, H-6', H-6''). 100 mg, 0.179 mmol of this compound was dissolved in DMF (2 ml) and sodium azide (41 mg, 0.628 mmol) was added. The resulting suspension was stirred at 60C for 3.5 h. The mixture was concentrated and the residue purified by column chromatography (toluene-acetone 2:1) (84 mg), (IR(CHC13) 2100 cm-l) this was dissolved in lM tetrabutylammonium fluoride x 3 H20 in THF (4 ml) at rot temperature. After 15 min, the mixture was concentrated and the residue purified by column chromatography (chloroform-methanol 5:1) and then HPLC (reversed phase, methanol-water 85:15) yielding the title compound (36 mg): ~] D
+54.4 (c 0.95, H20): IR(CHC12) 2100 cm~l, (azide): 13C N~R (D20, 40 C) 29.9 _(C-3'), 44.9 (C-2'), 52.6 (C-6', 64.0 (C-5'), 80.8 (C-4r), 90.0 (C-1'), 97.1 C-5), 142.6 (C-6), 158.3 (C-4), 166.9 (C 2); lH NMR (D~C, 40 C) 2.03 (m, 2H, H-3', H-3''), 2.61 (m, lH, H-2'), 3.56 (m, 2H, H-6', H-6''), 3.75 (m, 2H, H-5', H-5''), 4.33 (m, lH, H-4'), 5.92 (d, J= 5.50 Hz, lH, H-1'), 6.05 (d, J=7.32 Hz, lH, H-5), 7.82 (d, J=7.32 Hx, lH, H 6).
Anal. Calcd. for CloHl4O3N6: 45.11; H, 5.30; N, 31.56.
Found: C, 45.02; H, 5.41; N, 31.28.
~_~MPLE 23 1-[5'-O-Benzoyl-3'-C-~(benzoyloxy)methyl]-2',3'-dideoxy-4'-thio-~-and-~-D-erythro-pentafuranosyl]thyminet18).
A suspension of thymine (150~ mg, 1.19 ~mol) and a small crysta_ of ammonium sulfate in a mixtur2 of hexamethyldisilazane (2 ml) and trimethylchlorosilane (0.2 ml) was refluxed until a clean solution was obtained. Volatile matters were evaporated off and the residue was repeatedly co-evaporated with toluene. The resulting syrup was dissolved in dichloromethane (2 ml) under nitrogen. To this solution compound l6 (153 mg, 0.32 mmol) was added followed by the addition of tert.-butyldimethylsilyl triflate (0.075 mL, 0.33 mmol), mercuric ace ate (0.102 g, 0.32 mmol). The solution was stirred for 24 h at room temperature. The reaction was quenched by the addition of saturated aqueous sodium hydrogen carbonate, dried, concentrated and purified by column chromatograp~y (chloroform-ethyl acetate 1:1) to give tO.127 g, 87 ~!. NMR indicated ~hat .
. ,;
'.
W092/06102 2 0 9 3 0 2 ~ PCT/SE91/OOfiS3 -^
coupling of thymine had occurred but the spectra were too complex to analyse without separating the anomers. This material was de-O-benzoylated using sodium in methanol at room temperature to give 1(3'-C-hydroxymethyl)-2',3'-dideoxy 4'-thio-~-and-~-D-erythro-pentofuranosyl)thymine.
1-[5'-0-Benzoyl-3'-C-[(benzoyloxy)methyl]-2',3'-dideoxy-4'-thio-~-and-r~-D-erythro-pentofuranosyl]cytosine.
Cytosine (100 mg, O.gO1 mmol) was silylated following the same procedure as for the preparation of 18 and dissolved in acetonitrile (3 ml) under nitrogen. Compound 17 (119 mg, 0.287 mmol) was added followed by the addition of tert.-~~~~ butyldimethylsilyl triflate (0;13 ml, 0.574 mmol) and thesolution was stirred for 1 h at ooc. The reaction was quenched by the addition of saturated aqueous sodium hydrogen carbonate, stirred for 30 min, diluted with dichloromethane, washed with saturated aqueous sodium hydrogen carbonate, dried, concentrated and purified by column chromatography (ethyl acetate-methanol 4:1) to give an anomeric mixture of the protected nucleoside 15 (49 mg, 37 %). NMR indlcated that coupling of cytosine had occured but the spectra were too complex to analyse without separating the anomers. This material was de-O-benzoylated usinq sodium in methanol at roo~ temperature to give 1(3'-C-hydroxy~.ethyl)-2'-,3'-dideoxy 4'-thio-~-and-~-D-erythr~-pentofuranosyl)cytosine.
Ex~rimental The starting materials for the compounds in Exam~les 1-4 were prepared by the following sequence of reactions a - d:
a) (2S,3R)-l-O-p-Bromobenzyl-3-C-(2'-propenyl)-1,2,4-butantriol (compound 2, Scheme 1). To a cold solution of allylmagnesium bromide -50C (20 ml, lM) in 100 ml of dry diethyl ether under nitrogen atomosphere, a solution of t2S,3R)-3-[~(4-bromobenzyl)oxy]methyl~oxirane-2-methanol-1 (1.36 g, 5 mmol) in 140 ml of dry diethyl ether was added dropwise over 30 minutes. The mixture was vigorously stirred fo~
. . : ,. . . .
. . :.: : .. .
- : : , : ,: : ,: ;
::: . : : : :, , . ~ :',: . .. ~ :
WO92/06102 2 ~ 9 3 3 2 0 PCT/SE91/00653 30 minutes at -50C and then quenched with saturated aqueous ammonium chloride. The organic phase was collected and the aqueous phase was extracted with diethyl ether. The organic phases were combined and washed with hydrogen chloride (lM), sodium hydrogen carbonate (sat.), dri~d, filtered, concentrated and separated by column chromatography (toluene: ethyl acetate, 1:5), to give 2 (1 g, 64 %) ~and 3 (0.4 g, 25 %)], [~]22D: +1.56 (c 1.03, CHC13); lH-NMR (100 MHz, CDCl3): 1.8 (m lH, H-3); 2.13 (t, Jl~,2~ = Jl~,3 = 6-8 Hz, 2H, H-l'); 3-3 and 3.0 (broad, 2H, OH-2); 3.59 (m, 4H, H-1, H-4); 4.0 (m, lH, H-2); 4.48 (s, 2H, CH2Ph); 4.94 and 5.09 (m, 2H, H-3'a, H-3'b); 5.78 (m, lH, H-2');
7.14-7.5 (m, 4H, arom); 13C-NMR (25.05 MHz, CDC13); 30.6 (C-l');
42.3 (C-3); 63.3 (C-4); 71.9, 72.3, 72.5 (CH2Ph, C-1, C-2');
116.4 (C-3'); 121.5, 129.1, 131.3 (aromatic C); 136.4 (C-2 and aromatic C);
b~ (2S,3R)-4-O-Benzoyl-1-O-p-bromobenzyl-3-C-(2'-propenyl)-1,2,4-butan-triol (compound 4, scheme 1). Benzoyl chloride (3.21 ml, 27.6 mmol) was added dropwise to a solution of compound 2 (8.54 g, 27 mmol) in dry pyridine (50 ml) at 0C.
The reaction mixture was stirred at 0C for 15 minutes. Water (5 ml) was added and the solvent was evaporated. The residue was dissolved in dichloromethane and washed with hydrochloric acid (1 M), aqueous sodium hydrogen carbonate (sat.), dried, concentrated and purified by flash chromatography (toluene:
ethyl acetate, 2:1) to give compound 4 (9.77 g, 86 %). [~]22D:
+8.5 (c 0.71, CHCl3); lH-NMR (100 MHz, CDCl3): 1.95-2.43 (m, 3H, H-3, H-l'); 2.59 (d, JH(O~) ~ = 3-9 Hz, lH, OH-2); 3.54 (m, sec.
order, 2H, ~-la, H-lb); 3.95 (m, lH, H-2); 4.34 (d, J4 3 = 5.1 Hz, 2H, H-4); 4.48 (s, 2H, CH2Ph); 5.14 and ~.0 (m, 2H, H-3'a, H-3'b); 5.78 (m, lH, H-2'); 8.1-7.14 (m, 9H, ~rom.); 13C-NMR
(25.05 MHz, CDC13); 31.3 (C-l'; 40.4 (C-3); 70.1 C- 2; 64.0 (C-4); 7203 (CH2Ph); 72.5 (C-1); 116.3 (C-3'); 121.4-134.7 (arom.); 136.4 (C-2'); 166.1 (COPh).
c) Methyl 3-C-[(benzoyloxy) methyl]-5-O-p-bromobenzyl-2,3-dideoxy-D-erythro-pentofuranoside (compound 5, scheme 1). To an ice cold mixture of compound 4 (7.5 g, 17.9 mmol) and N-methylmorpholine-N-oxide (4.8 g, ~5.5 mmol) in ~ : :
..
.. ..
., .~ . . .
...
.
::
WO'~2/06102 2 0 9 3 9 2 U PCT/SE91/00653 --tetrahydrofuran:water (3:1, 70 ml), OsO4 in t-butanol (18 ml, 0.02 M, stab. with 1 % TBHP, 0.36 mmol) was added. After a few minutes, the ice bath was removed and the reaction mixture was stirred overnight at room temperature under nitrogen. NaHSO3 (2 g) was added and the mixture was stirred for 15 minutes. The solvent was evaporated off and the residue diluted with ethyl acetate, washed with HCl (1 M), NaHCO3 (sat.), dried, filtered and concentrated. The crude product was dissolved in tetrahydrofuran:water (3:1, 200 ml) and treated with NaIO4 (7.65 g, 35.8 mmol). The cis diol was completely claaved a~ter 30 minutes at room temperature. The tetrahydrofuran was evaporated off and the a~ueous residue was saturated with NaCl, and extracted with diethyl ether. The organic phase was dried and concentrated. The residue was treated with methanolic HCl (0.05 . . .
~, 50 ml) for ten minutes, neutralized with Dowex 2x8 (HCO3), filtered, evaporated and the residue was purified by flash chromatography (toluene-ethyl acetate, 3:1) to give an anomeric mixture of co~pound 5 (6.63 g, 85 %) as a colourless syrup.
lH-NMR (100 MHz, CDC13): 1.7-2.9 (three m, 3H, H-3, H-2a, H-2b);
3.31, 3.35 (2s, 3~1, OCH3); 3.6 (m, 2H, H-5); 4.1 (m, lH, H-4);
4.4 (m, 2H, H-6); 4.6 (m, 2H, CH2Ph); 5.1 (m, lH, H-1); 7.1-8~0 (m, 9H, aromatic); 13C- NMR (25.05 MHz, CDCl3): 35.6, 36.4 (C-2);
38.7, 39.3 (C-3); 54.3, 54.5 (OCH3); 65.7, 66.6, (C-6); 71.5, 72.35, 72.37, 73.8 (C-5) (CH2Ph); 79.9, 80.1 (C-4); 104.8 (C-1);
121.0- 136.4 (aromatic); 165.8 (COPh).
d) Methyl 5-O-benzoyl-3-C-[(benzoyloxy)methyl]-2,3,-dideoxy-D-erythro-pentafuranoside (compound 6, scheme 1). A
solution of compound 5 (1 g, 2,3 mmol) in dry diethyl ether (3 ml) was dissolved in liquid ammonia (50 ml) in a dewar bottle.
Sodium (300 mg, 13 mmol) was added in portions over 5 minutes.
The solution was stirred for 30 minutes and then quenched with solid NH4Cl. The ammonia was evaporated under a stream of nitrogen and the solid residue was diluted with ethyl acetate.
The solid was filtered off and washed several times with ethyl acetate. The filtrate was concentrated and then co-evaporated with dry toluene. The crude residue was dissolved in dry pyridine (30 ml). Benzoylchloride (0.8 ml, 6.9 mmol) was added and the solution was stirred for 40 minutes at room temperature :;
.. ,. . . . ,:
: . .:. . ..
: :. :: . , .
: . . ~
., ~
2~020 - , WO92/06102 PCT/SE91/~0653 after which water (5 ml) was added and the mixture was concentrated to dryness. The residue was dissolved in CH2Cl2 and washed with aqueous HCl (1 M), aqueous NaHC03 (sat.), dried, filtered and evaporated to dryness. Flash chromatography (toluene ethyal acetate, 3:1) yielded compound 6 (580 mg, 68 %), as an anomeric mixture. A small sample of compound 6 was separated by silica gel. lH-NMR and m.p. of the ~-anomer were in agreement with those previously reported.
The starting materials for the compounds in Exam~les 11-16 were prepared by the following sequence of reactions a-e:
a) Methyl 5-0-(p-bromobenzyl)-3-C-(hydroxymethyl)-2,3-dideoxy-D-erythro-pentofuranoside. Methyl 3-C-[(benzoyloxy]-5-0-(~-bromobenzyl)-2,3-dideoxy-D-erythro-pentofuranoside (1.61 mg, 3.70 mmol) was treated with methanolic ammonia (30 ml, saturated) for 24 h. The solvent was evaporated, and the residue was purified by flash column chromatography (toluene-ethyl acetate, 1:2) to give the title compound ~1.14 g, 93 %). Anal.
Calcd for C14H1904Br: 50.77; H, 5.78. F'ound: C, 50.67; H, 5.73.
b) Methyl 5-0-tp-bromobenzyl)-3-C-(fluoromethyl)-2,4-dideoxy-D-erythro-pentofùranside. To a cold solution (-15C) of the previous compound (637 mg, 1.92 mmol) in dichloromethane (15 ml) and pyridine (0.31 ml, 3.85 mmol) under nitrogen, a solutlon of trifluoromethanesulfonic anhydride (0.38 ml, 2.26 mmol) in dichloromethane (5 ml) was added dropwise. After stirring for lo min at -15C, the mixture was diluted with dichloromethane (100 ml), washed with l M hydrogen chloride, saturated aqueous sodium hydrogen carbonate, dried, and concentrated at a bath temperature not exceeding 20C. The residue was treated with a solution of anhydrous tetrabutylammonium fluoride in tetra hydrofuran (6 ml, lM) for 15 min. The mixture was concentrated and the residue was subjected to flash column chrom~tography (toluene-ethyl acetate, 9:1) to give the title compound (466 mg, 73 %). Anal. Calcd for C14H1803BrF: 50.46; H, 5.45. Found: c, 50.28; H.35.
,. ~ .
,.: ., ; . , : , WO~2/06102 2 Q 9 3 0 2 ;~ PCT/SE91/00653 c) Methyl 5-p-benzoyl-3-C-(fluoromethyl)-2,3-dideoxy-D-erythro-pentofuranoside. A mixture of the previous compound (572 mg, 1.72 mmol) in ethanol (40 ml) containing sodium hydrogen carbonate (excess) and 10 % Pd on charcoal (50 mg) was treated with hydrogen for 5 h at ambient pressure. The solids were filtered off and the filtrate was concentrated. The crude residue was dissolved in a mixture of dichloromethane (3 ml) and pyridine (0.3 ml, 3.7 mmol), followed by the addition of benzoyl chloride (0.24 ml, 2.07 mmol). The reaction mixture was stirred for 15 min. Water (2 ml) was added, and the mixture was stirred for 10 min, diluted with dichloromethane, washed with 1 M
hydrogen chloride, saturated aqueous hydrogen carbonate, dried, and concentrated. The residue was purified by flash column chromatography (toluene-ethyl acetate, 4:1) to give the title compound (372 mg, 81 ~). Anal. Calcd. for Cl~Hl7O4F: C, 62.68; H, 6.39. Found: C, 62.55; H, 6.33.
d) Methyl 3-C-(azidomethyl)-5-O--(p-bromobenzyl)-2,3-dideoxy-D-erythro-pentofuranoside.
To a mixture of methyl 5-O-(p-bromobenzyl-3-C-(hydroxymethyl)-2',3'-dideoxy-D-erythro-pentofuranoside (1.95 g, 5.89 mmol), triphenylphosphine (1.6 g, 6.1 mmol), and lithium azide (1.5 g, 30.6 mmol) in dry N,N-dimethylformamide (25 ml) was added carbon tetrabromide (1.95 g, 5.89 mmol) at room temperature. The mixture was vigorously stirred for 24 h.
~ethanol (3 ml) was added, and the solvent was evaporated. The mixture was diluted with dichloromethane, washed with water, dried, and concentrated. The residue was purified by flash column chromatography (toluene-ethyl acetate, 4:1) to give the title compound (1.99 g, 95 %) ~maX 2100 cm-1. Anal. Calcd. for C14~l8O3BrN3: C, 47.20; H, 5.09; N, 11.80. Found;C, 47.15; H, 4.98; N, 11.9.
e) Methyl 3-C-(azidomethyl)-5-O-(benzoyl)-2,3-dideoxy-D-erythro-pentofuranoside. To a stirred solution of dry pyridine (4.7 ml, 58.3 mmol) and dichloromethane (20 ml) was added chromium trioxide (2.8 g, 28.0 mmol). The mixture was stirred for 15 min at room temperature. A solution of the previous compound (1.68 g, 4.72 mmol) in dichloromethane (20 ml) was : . .
: . ~ .~ . . .
: :.: ~
W O 92/06102 2 Q ~ 3 0 2 a P ~ /SE91/00653 added, followed by acetic anhydride (3.2 ml, 32.0 mmol), and the mixture was stirred for 10 min at room temperature. The mixture was passed through a short column of silica gel with ethyl acetate as eluent, to give a crude product, which was treated with methanolic ammonia (30 ml, saturated) for 24 h. The solvent was evaporated, and the residue was purified by flash column chromatography (toluene-ethyl acetate, 1:1) and then dissolved in a mixture of dichloromethane (15 ml) and pyridine (1 ml, 12.4 mmol), after which benzoyl chloride (0.55 ml, 4.73 mmol) was added. The reaction mixture was stirred for 15 min.
Water (2 ml) was added, and the mixture was stirred for 10 min, diluted with dichloromethane, washed with 1 M hydrogen chloride, saturated aqueous hydrogen carbonate, dried, and concentrated.
The residue was purified by flash column chromatography (toluene-ethyl acetate, 9:1) to give the title compound (1.0 g 57 %). Anal. Calcd for C14H17O4N3: C, 57.72; H, 5.88; N, 1~.43.
Found: C, 57.86; H, 5.74; N, 14.43.
The starting material for the compounds in Example_17 were prepared by the following sequence of reactions a b:
a) (3S,4S~-3,4-di(benzoyloxymethyl)cyclopentanone (compound 8, Scheme 2). A mixture of (-)(3S,4S)-3,4-di(methoxycarbonyl)-cyclopentanone (compound 7, Scheme 2) (0.93 g, 4.6 mmol), ethylene glycol ~6,5 ml, 0.12 mol) and p-toluenesulfonic acid monohydrate (25 mg) in toluene (50 ml) was refluxed for 6 h with a Dean-Stark trap. Sodium hydrogen carbonate (20 mg) was added and after stirring for 5 min the mixture was washed with saturated aqueous sodium hydrogen carbonate, dried (MgS04) and concentrated to a cr-lde ketal diester. According to lH NMR, the product was free from unreacted ketone. The crude ketal diester in dry diethyl ether (15 ml) was added dropwise for 1 h to an ice-cold mixture of lithium aluminium hydride ~0~35 g, 9.2 mmol) in dry diethyl ether (35 ml). The mixture was stirred at room temperature for 3 h before the excess of lithium aluminium hydride was decomposed by successive addition of water (0.5 ml), 3 M aqueous NaOH (0.5 ml) and water (1.5 ml). After stirring for 1 h MgSO4 (20 g) was added and the stirring was prolonged for 5 min. The precipitate and MgSO4 were remov~d by filtration and washed several times .
.
WO92/06102 2 ~ ~ 3 0 2 ~ 38 PCT/SE91/00653 ~
with ethyl acetate. The filtrate was concentrated to give a crude oil of the diol. To a stirred solution of the diol in pyridine (13.5 ml) was added benzoyl chloride (2.3 ml, 2001 mmol) dropwise. The mixture was stirred for 3 h in room temperature. Water (5 ml) was added and after stirring for 10 min, the mixture was diluted with saturated aqueous sodium hydrogen carbonate and dichloromethàne. The organic layer was separated and washed with saturated sodium hydrogen carbonate, dried (MgSO4) and concentrated. Column chromatography (toluene-EtOAc, 3:1) on a short column of silica gel gave a syrupy residue including the dibenzoylated Xetal and traces of the dibenzoylated ketone. The syrup was dissolved in methanol (9~
ml) and 2 M aqueous HCl (31 ml), stirred at room temperature for 2 h and then at 50C for additional 2 h. The solution was neutralized with sodium hydrogen carbonate (5.2 g), diluted with water, extracted with dichloromethane. The organic layer was dried (MgSO4), concentrated and crystallized from diethyl ether/hexane to give the title compound as needles (0.90 g, 55%): m.p. 85-86C; [~]D-59.1 (c 1.04, chloroform);; lH NMR (100 MHz, CDC13) ~ 2.12-2.73 (m, 6H, 2 CH, 2 CH2), 4.50 (d, 4H, 2 CH2-OBz), 7.36-7.64 and 7.94-8.07 (m, 10H, aromatic H); 13C NMR
(25.05 MHz, CDC13) ~ 38.3 (2 CH2), 41.6 (2 CH) 65.9 (2 CH2-OBz), 128.2, 128.2 and 133.0 (aromatic C), 165.9 and 214.9 (C=O).
Anal. Calcd for C21H20O5 (352.4): C, 71.58; H, 5.72. Found: C, 71.34; H, 5.73.
b) (3s,4S)-3,4-di(benzoyloxymethyl)cyclopentanol (compound 9, Scheme 2). To the compound 8 obtained under a) (193 mg, 0,55 mmol) in methanol (10 ml) was added sodium borohydride (41 mg, 1,10 mmol~ in successive portions. The mixture was stirred at room temperature for 30 min, and then diluted with water and diethyl ether. The organic layer was washed with saturated agueous sodium chloride r dried (MgSO4) and concentrated to a syrup which was purified by column chromatography on silica gel (toluen/EtOAc, 1 1) to yield title compound (189 mg, 97%; [~]D
_16.4 [c 1.09, chloroform); 1H NMR (100 NHz, CDC13) ~ 1.6-2.7 (m, 7H, 2 CH, 2 CH2, OH), 4.41 ~t, 4H, 2 CH2-OBz), 7.34-7.56 and 7.97-8.09 (m, 10H, aromatic H); 13C NMR (25.05 MHz, CDC13) ~
38.3, 39.3, 39.8, 40.0 (2 CH2 2 CH), 67.4, 68.2 (2 CH2-OBz), 72.5 - .. .. ., ~.,.,.. ~ , .
.: ~ :, . - .:
,~
WO ~2/06102 ~5i3 ~ 3 o 2 ~ PCI/SE91/û0653 (CHOH), 128.1, 129.3, 129.8, 132.8 ~aromatic C), 166.3 tC=O)-Anal. Calcd for C2lH22O5 (354.4): C, 71.17; H, 6.26. Found: C, 71.05; H, 6.18.
The starting materials for the compounds in Examples 18 - 22 were prepared by the following sequence of reactions a-c:
a) (S)-4-tert-Butyldiphenylsilyloxy)methyl-(R,S)-2-hydroxymethyl-~-buty-- ~lactone (11). Ethanol (28 ~Ll, 0.48 mmol) was added to sodium (~80 mg, 12.17 mmol) in dry diethyl ethe~ (5 ml), and the mixture was stirred at room temperature for 2 h. A
solution of (S)-4-(tert-butyldiphenylsilyloxy)methyl-~-butyrolactone (3.50 g, 9.87 nunol) and ethyl forma~e (980 mg, 13.23 mmol) in diethyl ether (6 ml) was added and the stirring was continued for 16 h at room temperature. Diethyl ether (20 ml) and 1 M NaH2PO4 (10 ml) were added and the phases were separated. The organic phase was washed with water, dried and concentrated. ~he residue was dissolved in ethanol tl0 ml), and a solution of NaBH4 (500 mg, 13.22 mmol) in ethanol (10 ml) was added. After 5 min, the reaction was 1:erminated by the addition of a few drops of 80 % acetic acid. The mixture was partitioned between ethyl acetate and water. The organic phase was dried, concentrated and the residue purified by column chromatography (toluene-acetone 9:1) yielding 11 (3.40 g, 90 %).
Anal. Calcd for C22H2~o4Si: C, 68.71; H, 7.34. Found:
C, 68.58; H, 7.36.
b) (S)-2-Acetoxymethyl-(S)-4~(tl~rt-butyldiphenyl-silyloxy)methyl-~-butyrolactone( 12) . A solution of 11 (2.00 ~, 5.20 mmol) in pyri.dine (20 ml) and acetic anhydride (~0 ml) was stirred at 60C for 30 min. The solut on was concentrated and the residue purified by column chromatography (toluene-acetone 1) to give 12 (1.41 g, 64 %-): [~]D +22.4 (c: 1.00, CHCl3): 13c ~,X (CDCl3) ô 29.3 (C, tert); 20.8 (CH3, acetate), 26.9 (3 x CH3), 27.3 (C-3), 39.8 ~C-2~, 63.1, 65.7 (CH20Ac, C-5), 78.1 (C-4), 127.9, 128.3-135.7 (ArC), 170.7 (acetate), 176.4 (carbonyl~;
lH NMR (CDCl3) ~ 1.05 (s, 9H, 3 x CH3), 2.07 (s, 3H, CH3, acetate), 2.39 (m, 2H, H-3, H 3') j 3.13 (m, lH, H-2), 3.79, 4.35 (m, m, 4H, H-5, H-5', H-6, H-6'), 4.58 (m, lH, H-4), 7.17-7.72 ... .. .
. .
, , -': . ~ '` ., ': ' WO92/06102 2 0 9 3 0 2 ~ PCT/SE91/006~3 -(m, lOH, ArH).
Anal. Calcd. for C24H30o5si: C, 67-57; H, 7-09- Found:
C, 67.48; ~, 7.15.
c) 2-C-Acetoxymethyl-l-O-acetyl-5-O-(tert-butyldiphenyl)-silyl-D-erythro-pentofuranose (13). To a solution of lactone 12 (500 mg, 1.17 mmol) in toluene (30 ml) at ~78C was added diisobutylaluminium hydride (20 ~ in hexane, 3.00 ml, 2.95 mmol). The solution was stirred for 1.5 h, allowed to warm to room temperature, and methanol (0.5 ml) was added. To the solution was added ethyl acetate (30 ml) and aqueous sat. NaHC03 (4 ml). After stirring for 2 h, dry MgS04 (2.0 g) was added and after additional stirring for 3 h, the mixture was filtered. The filtrate was concentratPd, the residue dissolved in pyridine (6 .
ml) and acetic anhydride (3 ml) and heated to 40C for 30 min, concentrated and the residue purified by column chromatosraphy (toluene-acetone 10:1) to yield the title compound (500 mg, 91 %) as an anomeric mixture. 13C NMR (CDCl3, selected signals) 19.4 (C, tert.), 20.9, 21.3, 21.4 (CH3, acetates), 26.9 (3 x CH3), 28.4 (C-3), 42.3, 44.8 (C-2), 62.8, 63.7, 65.8, 66.8 (C-5 and C-6), 80.0, 81.4 (C-4), 97.6, 100.2 (C-1), 127.8-135.7 (ArC), 170.2, 170.9 (acetates).
Anal. Calcd. for C26H3406Si: C, 66.35; H, 7.29. Found:
C, 66.51; ~, 7.28.
The starting materials for the compounds in Examples 23 - 24 were prepared by the following sequence of reactions a-c:
a) 5-0-Benzoyl-3-C~[(benzoyloxy)methyl]-2,3-dideoxy-L-threo-pentose dibenzyl dithioacetal (lS).
Compound 14 (0.191 g, 0.516 mmol) was treated at room temperature with benzyl mercaptane (0.24 ml, 2.07 mmol) in dichloromethane (3 ml) containing a catalytic amount of stannic chloride for 24 hours. ThP mixture was then diluted with dichloromethane and washed with saturated aqueous sodium hydrogen sulfate, dried, filtered, concentrated and purified by flash column chromatography (toluene-ethyl acetate 9:1) to give compound 15 (0.266 g, 88 ~) as a colourless syrup: [~]22D-8.1 (c 1.0, CHCl3). 13C NMR (25.05 MHz, CDCl3) ~ 34.0, 34.6, 34.9 (C-2, ; . ~:: . : :. ;,, . , :
-. . : : :; , .. :
W092/06102 2 ~ ~ 3 Q 2 a PCT/SE91/00653 ~1 CH2Ph), 38.9 (C-3), 47.9 (C-1), 63.2 (C-4), 67.6 (C-5), 69.8 (c-6), 127.1-138.0 (aromatic), 166.7 (COPh).
b) Benzyl 5-O-benzoyl-3-C-[(benzoyloxy)methyl?-2,3-dideoxy-1,4-dithio-a-and-B-D-erythro-pentofuranoside (16). To a solution of compound 1~ (0.347 g, 0.592 mmol) in tolu ne-acetonitrile (2:1, 9 ml) were added triphenylphosphine (0.93 g, 3.55 mmol) and triiodoimidazole (0.79 g, 1.78 mmol). The mixture was kept at 100C over night. The mixture was diluted with toluene and washed with saturated aqueous sodium hydrogen sulfate, dried, filtered, concentrated and purified by column chromatography (toluene-ethyl acetate 50:1) to give 16 (0.219 g, 77 %) as a colourless syrup: [~22D-4.2 (c 1.0, CHCl3); 1H NMR
(100 MHz, CDCl3) ~ 2.0-2.3 (m, 2H, H-2), 2.8 (m, lH, H-3), 3.8 (m, 3H, CH2Ph, H-4~, 4.5 (m, 5H, H-1, H-5, H-6), 7.1-8.1 (m, 15H, aromatic).
c) Acetyl 5-O-benzoyl-3-C-[(benzoyloxy)methyl]-2,3-dideoxy-4-thio-~-and-~-D-erythro-pentofuranoside tl7). Compound 16 (0.182 g, 0.381 mmol) and mercuric acetate (0.243 g, 0.761 mmol) in glacial acetic acid (5 ml) were stirred at room temperature for 30 min. The solvent was evaporated and co~
evaporated with dry toluene. The residue was diluted with dichloromethane, filtered through celite and concentrated. Flash chromatography (toluene-ethyl acetate 9:1) gave compound 17 (0.156 g, 99 -~ as a colourless syrup: lH NMR (100 MHz, CDCl3) ~
2.03, 2.07 (2s, 3H, COOCH3), 2.3 (2H, H-2), 3.0 (m, 1~, H-3), 3.8 (m, lH, H-4), 4.4 (m, 4H, H-5, H-6), 6.2 (m, lH, H--1), 7.0-7.9 (m, 10H, aromatic). ` `
Test I. Effect of compounds of the formula I on HIV in H9 cells HIV infection_of H9 cells H9 cells, 105 cells per well on a 24 well plate, suspended in 2 ml RPMI-medium containing 10 % fetal calf serum, 100 mg/ml penicillin, 10 mg/ml streptomycin sulfate and 2 mg/ml polybrene ar~ exposed to HIV (HTLV-IIIB) and different , .; , . . : ..... :
. .
. , .: :
, -W O 92/06102 2 0 9 3 0 2 ~ P ~ /SE91/00653 concentrations of the test compounds. The plates are incubatedat 37C in 5 % C02 for 6-7 days. The contents in each well is then homogenized with a pipette and transferred to a centrifuge tube. After centrifugation for 10 min at 1500 rpm the supernatant is removed and the cell pellet is analyzed by fixing in methanol on glass plates. Human HIV positive serum diluted 1:80 or 1:160 is added and incubated for 30 min at 37C. The plate is then washed with phosphate-buffered saline (P8S) containing Ca2+ and Mg2+. Sheep antihuman conjugate (FITC) is added and after a new incubation the plate is again washed with PBS. Contrast staining is done with Evans blue and after drying the frequency of HIV antigen containing cells is determined in a microscope. The test result is shown in Table 1.
Table 1 Concentration (m~) for 50 % i~hibition ~ICso~ of human immuno deficiency virus multiplication in cell culture _________________________________________________________~______ Compound IC50 ~M
________________ _______________________________________________ 1-[2',3'-Dideoxy-3'-C-(hydroxymethyl)-~-D--erythro-pentafuranosyl]cytosine 0.01 1-[2',3'-Dideoxy-3'-C-(hydroxymethyl)-~-D- ' -erythro-pentafuranosyl]cytosine 5 1-[2',3'-Dideoxy-3'-C-(hydroxymethyl) ~-D--erythro-pentAfuranosyl]thymine 5 1-[2',3'-Dideoxy-3'-C-(hydroxymethyl)-~-D--erythro-pentafuranosyl]thymine 10 r 9-[2',3'-Dideoxy-3'-C-(hydroxymethyl)~-D--erythro-pentafuranosyl]adenine 10 _____..__________________________________________________________ Table 1 shows that the tested compounds are active inhibitors of HIV virus multiplication.
Test II. Cellular toxicity ,'' ', ~' , ; , ' ~ ' " ,:
:: . :
W O 92/06102 2 ~ ~ 3 O 2 ~ PC~r/SE91/006~3 H9 cells, 2x107 cells per plate, are incubated in RPMI-1640 medium containing 10 % fetal calf serum, 70 mg/l penicillin, 100 mg/l streptomycin and 10 mM hepes, in absence or presence of test compounds. The number of cells per plate is determined after 48 h. Cells incubated in the absence of test compounds then underwent two cell division cycles.
F5000 cells, Jhich are human embryo cells, lx105 cells per plate, are incubated in Eagle's minimal essential medium, supplemented with Earle's sals, non-essential amino ac,ds, 10 %
fetal calf serum, 10 mM hepes, 70 mg/l penicillin and 100 mg/l streptomycin, in absence or presence of test compounds. The number of cells per plate is determined after 48 h. Ce incubated in the absence of test compounds underwent c . -ell division cycle. The results are given as % inhibition . cell multiplication and are given in Table 2.
Table 2. Cellular toxicity on H9 ~lls __________________________________________________________~_____ Compound % Inhibition (H9) .____________________________________.__________________________ 1-[2',3'-Dideoxy-3'-(hydroxymethyl)-~--D--erythro-pentofuranosyl]cytosine (1 mM) 50 _____________~_______________________.__________________________ Table 2 shows that the concentration at which the compound exhibit toxicity exceed the concentrat ~n needed for 50 ~ inhibition of HIv multiplication as given in Table 1.
. , . ~ ,: , ~ , . .
Claims (28)
1. A compound of the formula wherein X is O, S, SO, SO2, CH2; R1 is OH, O-PO(OH)2, O-PO(OH)-O-PO-(OH)2, O-PO(OH)-O-PO(OH)-O-PO(OH)2 or (CH2)nOCH2PO(OH)2 wherein n is 0-2;
R2 is H and R3 is CH3, CH2OH, CH2OCH3, CH2SH, CH2F or CH2N3; or R3 is H and R2 is CH3, CH2OH, CH2OCH3, CH2SH, CH2F or CH2N3;
R4 is or Y is OH, NH2 and R5 is CH=CH2, C?CH, CH=CH-CH3, C?C-CH3, thien-2-yl, thien-3-yl, H, CH3, C2H5, CH2CH2CH3 or CH(CH3)2;
R6 and R7 are the same or different and are H, F, Cl, OH, NH2 or SH;
in the form of a mixture of .alpha.- and .beta.-anomers or in the form of an .alpha.- or .beta.-anomer; and pharmaceutically acceptable salts thereof, with the provisos:
a) In the combination wherein X is O, S; R2 is H and R3 is CH2OH; Y is OH, NH2; R5 is CH=CH2; C?CH, CH=CHCH3, H, CH3, C2H5, CH2CH2CH3, CH(CH3)2; then the claimed compounds of the formula 1A
only are these in the form of the .beta.-anomer.
b) In the combination wherein X is CH2; R2 is H and R3 is CH2OH; the claimed compounds of the formula 1A are those wherein R4 is and R6 and R7 are the same or different and are H, Cl, OH, NH2 or SH.
R2 is H and R3 is CH3, CH2OH, CH2OCH3, CH2SH, CH2F or CH2N3; or R3 is H and R2 is CH3, CH2OH, CH2OCH3, CH2SH, CH2F or CH2N3;
R4 is or Y is OH, NH2 and R5 is CH=CH2, C?CH, CH=CH-CH3, C?C-CH3, thien-2-yl, thien-3-yl, H, CH3, C2H5, CH2CH2CH3 or CH(CH3)2;
R6 and R7 are the same or different and are H, F, Cl, OH, NH2 or SH;
in the form of a mixture of .alpha.- and .beta.-anomers or in the form of an .alpha.- or .beta.-anomer; and pharmaceutically acceptable salts thereof, with the provisos:
a) In the combination wherein X is O, S; R2 is H and R3 is CH2OH; Y is OH, NH2; R5 is CH=CH2; C?CH, CH=CHCH3, H, CH3, C2H5, CH2CH2CH3, CH(CH3)2; then the claimed compounds of the formula 1A
only are these in the form of the .beta.-anomer.
b) In the combination wherein X is CH2; R2 is H and R3 is CH2OH; the claimed compounds of the formula 1A are those wherein R4 is and R6 and R7 are the same or different and are H, Cl, OH, NH2 or SH.
2. A compound of the formula wherein X is O, S, SO, SO2, CH2; R1 is OH, O-PO(OH)2, O-PO(OH)-O-PO-(OH)2, O-PO(OH)-O-PO(OH)-O-PO(OH)2 or (CH2)nOCH2PO(OH)2 wherein n is 0-2;
R2 is H and R3 is CH3, CH2OH, CH2OCH3, CH2SH, CH2F or CH2N3; or R3 is H and R2 is CH3, CH2OH, CH2OCH3, CH2SH, CH2F or CH2N3;
R4 is or Y is OH, NH2 and R5 is CH=CH2, C?CH, CH=CH-CH3, C?C-CH3, thien-2-yl, thien-3-yl, H, CH3, C2H5, CH2CH2CH3 or CH(CH3)2;
R5 and R7 are the same or different and are H, F, Cl, OH, NH2 or SH;
in the form of a mixture of .alpha.- and .alpha.-anomers or in the form of an .alpha.- or .beta.-anomer; and pharmaceutically acceptable salts thareof.
R2 is H and R3 is CH3, CH2OH, CH2OCH3, CH2SH, CH2F or CH2N3; or R3 is H and R2 is CH3, CH2OH, CH2OCH3, CH2SH, CH2F or CH2N3;
R4 is or Y is OH, NH2 and R5 is CH=CH2, C?CH, CH=CH-CH3, C?C-CH3, thien-2-yl, thien-3-yl, H, CH3, C2H5, CH2CH2CH3 or CH(CH3)2;
R5 and R7 are the same or different and are H, F, Cl, OH, NH2 or SH;
in the form of a mixture of .alpha.- and .alpha.-anomers or in the form of an .alpha.- or .beta.-anomer; and pharmaceutically acceptable salts thareof.
3. A compound according to any of claims 1 - 2 wherein X is 0.
4. A compound according to any of claims 1 - 2 wherein X is S, SO or SO2.
5. A compound according to any of claims 1 - 2 wherein X is CH2.
6. A compound according to any of claims 1 - 5 wherein R1 is OH; R2 is H and R3 is CH2OH; R3 is H and R2 is CH2OH.
7. A compound according to any of claims 1-5 wherein R1 is OH;
R2 is H and R3 is CH3, CH2SH, CH2OCH3, CH2F, CH2N3; R3 is H and R2 is CH3, CH2SH, CH2OCH3, CH2F, CH2N3.
R2 is H and R3 is CH3, CH2SH, CH2OCH3, CH2F, CH2N3; R3 is H and R2 is CH3, CH2SH, CH2OCH3, CH2F, CH2N3.
8. A compound according to any of claims 1-5 wherein R1 is (CH2)nOCH2PO(OH)2 and n is 0 - 2.
9. A compound according to any of claims 1-8 wherein X is OH, NH2 and R5 is H, CH3, CH=CH2, C?CH or CH=CH-CH3.
10. A compound according to any of claims 1-8 wherein R6 is NH2 when R7 is H, OH, SH or NH2.
11. A compound according to any of claims 1-8 wherein R6 is H, F
or Cl when R7 is OH, SH or NH2.
or Cl when R7 is OH, SH or NH2.
12. A compound according to any of claims 1-8 wherein R6 and R7 are OH.
13. A compound according to any of claims 1-12 in the form of an .alpha.-anomer.
14. A compound according to any of claims 1-12 in the form of a .beta.-anomer.
15. A compound of the formula 1A and 1B according to any of claims 1-14 for use in therapy.
16. A pharmaceutical composition comprising as an active ingredient a compound of the formulas 1A and 1B according to any of claims 1-14 and a pharmaceutically acceptable carrier, including liposomes.
17. A method for treatment of virus infections in an animal or human host in need of treatment, comprising administering an effective amount of a compound of the formulas 1A and 1B as defined in any of claims 1-14.
18. A method according to claim 17 for treatment of infections caused by viruses requiring reverse transcriptase for replication, including human immuno deficiency viruses and hepatitis B virus.
19. A method according to claim 17 for treatment of infections caused by herpes viruses.
20. A method according to claim 17 for treatment of infections caused by parasites.
21. Use of a compound of the formula 1A and 1B according to any of claims 1-14 for the manufacture of a medicament for treatment of the acquired immuno deficiency syndrome and infections caused by viruses requiring reverse transcriptase for replication.
22. Use according to claim 21 for the treatment of infections caused by HIV-viruses.
23. Use according to claim 21 for the treatment of infections caused by hepatitis B virus.
24. Use according to claim 21 for the treatment of infections caused by herpes viruses.
25. Use according to claim 21 for the treatment of infections caused by parasites.
26. A process for preparation of a compound of the formula 1A
wherein X, R1, R2, R3 and R4 are as defined in claim 1, by condensing a glycoside as comprised in the formula to the N-1 position of a pyrimidine derivative or to the N-9 position of a purine derivative 1A
wherein Z is Cl, Br, J, acyloxy or alkoxy. R1', R2' and R3' are R1, R2 and R3 respectively as defined above or with the proviso that when R1 or R2 is OH then O must have a protecting group, R4' is R4 as defined above, having a silyl, acyl or alkyl protecting group.
wherein X, R1, R2, R3 and R4 are as defined in claim 1, by condensing a glycoside as comprised in the formula to the N-1 position of a pyrimidine derivative or to the N-9 position of a purine derivative 1A
wherein Z is Cl, Br, J, acyloxy or alkoxy. R1', R2' and R3' are R1, R2 and R3 respectively as defined above or with the proviso that when R1 or R2 is OH then O must have a protecting group, R4' is R4 as defined above, having a silyl, acyl or alkyl protecting group.
27. A process for the preparation of a compound of the formula 1B
wherein X, R1, R2 and R3 are as defined in claim 1, by condensing a glycoside as comprised in the formula to the N- 1 position of a pyrimidine derivative or to the N-9 position of a purine derivative 1B
wherein Z is Cl, Br, J, acyloxy or alkoxy. R1', R2' and R3' are R1, R2 and R3 respectively as defined above or with the proviso that when R1 or R2 is OH then O must have a protecting group, R4' is R4 as defined above, having a silyl, acyl or alkyl protecting group.
wherein X, R1, R2 and R3 are as defined in claim 1, by condensing a glycoside as comprised in the formula to the N- 1 position of a pyrimidine derivative or to the N-9 position of a purine derivative 1B
wherein Z is Cl, Br, J, acyloxy or alkoxy. R1', R2' and R3' are R1, R2 and R3 respectively as defined above or with the proviso that when R1 or R2 is OH then O must have a protecting group, R4' is R4 as defined above, having a silyl, acyl or alkyl protecting group.
28. A process for preparation of a compound of the formula wherein X, R1, R2 and R3 are as defined in claim 1 and Z is Cl, Br, J, acyloxy or alkoxy, by reacting a protected butan-1,4-diol-2,3 epoxide with a nucleophile containing 3 or 4 carbon atoms with a double bond, followed by transforming the double bond and ring closure.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE9003151A SE9003151D0 (en) | 1990-10-02 | 1990-10-02 | NUCLEOSIDE DERIVATIVES |
| SE9003151-9 | 1990-10-02 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2093020A1 true CA2093020A1 (en) | 1992-04-03 |
Family
ID=20380539
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002093020A Abandoned CA2093020A1 (en) | 1990-10-02 | 1991-09-27 | Nucleoside derivatives |
Country Status (11)
| Country | Link |
|---|---|
| US (4) | US5473063A (en) |
| EP (1) | EP0554274A1 (en) |
| JP (1) | JPH06501261A (en) |
| KR (1) | KR930702371A (en) |
| AU (1) | AU8641091A (en) |
| CA (1) | CA2093020A1 (en) |
| HU (1) | HUT63851A (en) |
| IE (1) | IE913374A1 (en) |
| PT (1) | PT99130A (en) |
| SE (1) | SE9003151D0 (en) |
| WO (1) | WO1992006102A1 (en) |
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| US5521163A (en) * | 1990-07-13 | 1996-05-28 | University Of Birmingham | Antiviral pyrimidine nucleosides and methods for using same |
| SE9003151D0 (en) * | 1990-10-02 | 1990-10-02 | Medivir Ab | NUCLEOSIDE DERIVATIVES |
| US5444063A (en) * | 1990-12-05 | 1995-08-22 | Emory University | Enantiomerically pure β-D-dioxolane nucleosides with selective anti-Hepatitis B virus activity |
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| JP3739785B2 (en) * | 1991-11-26 | 2006-01-25 | アイシス ファーマシューティカルズ，インコーポレイティド | Enhanced triple and double helix shaping using oligomers containing modified pyrimidines |
| US6235887B1 (en) | 1991-11-26 | 2001-05-22 | Isis Pharmaceuticals, Inc. | Enhanced triple-helix and double-helix formation directed by oligonucleotides containing modified pyrimidines |
| GB9218810D0 (en) * | 1992-09-04 | 1992-10-21 | Univ Birmingham | Antiviral pyrimidine nucleosides |
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| US5808040A (en) * | 1995-01-30 | 1998-09-15 | Yale University | L-nucleosides incorporated into polymeric structure for stabilization of oligonucleotides |
| PT831852E (en) | 1995-06-07 | 2007-02-28 | Uab Research Foundation | Nucleosides with anti-hepatitis b virus activity |
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| GB9600143D0 (en) | 1996-01-05 | 1996-03-06 | Wellcome Found | Therapeutic compounds |
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| TWI637963B (en) | 2012-03-28 | 2018-10-11 | 富士軟片股份有限公司 | Salt of 1-(2-deoxy-2-fluoro-4-thio-β-d-arabinofuranosyl) cytosine technical field |
| PL2883866T3 (en) | 2012-08-13 | 2019-07-31 | Fujifilm Corporation | INTERMEDIATE FOR SYNTHESIS OF 1-(2-DEOXY-2-FLUORO-4-THIO-beta-D-ARABINOFURANOSYL) CYTOSINE, INTERMEDIATE FOR SYNTHESIS OF THIONUCLEOSIDE, AND METHODS FOR PRODUCING THESE INTERMEDIATES |
| JP6175553B2 (en) * | 2014-02-18 | 2017-08-09 | 富士フイルム株式会社 | Method for producing thiolane skeleton type sugar compound and thiolane skeleton type sugar compound |
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| CN107592864B (en) * | 2015-05-12 | 2021-04-16 | 豪夫迈·罗氏有限公司 | Novel substituted aminothiazolopyrimidinediones for the treatment and prevention of viral infections |
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| CA3089728C (en) | 2018-01-29 | 2023-01-10 | Fujifilm Corporation | Antitumor agent for biliary tract cancer and method for treating biliary tract cancer |
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| US3116282A (en) * | 1960-04-27 | 1963-12-31 | Upjohn Co | Pyrimidine nucleosides and process |
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| US4267171A (en) * | 1979-07-02 | 1981-05-12 | The Regents Of The University Of California | C-5 Substituted cytosine nucleosides |
| US4247544A (en) * | 1979-07-02 | 1981-01-27 | The Regents Of The University Of California | C-5 Substituted uracil nucleosides |
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| SE8701605D0 (en) * | 1987-04-16 | 1987-04-16 | Astra Ab | NOVEL MEDICINAL COMPOUNDS |
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| US5521161A (en) * | 1993-12-20 | 1996-05-28 | Compagnie De Developpment Aguettant S.A. | Method of treating HIV in humans by administration of ddI and hydroxycarbamide |
-
1990
- 1990-10-02 SE SE9003151A patent/SE9003151D0/en unknown
-
1991
- 1991-09-26 IE IE337491A patent/IE913374A1/en unknown
- 1991-09-27 EP EP91917480A patent/EP0554274A1/en not_active Ceased
- 1991-09-27 KR KR1019930701006A patent/KR930702371A/en not_active Application Discontinuation
- 1991-09-27 HU HU93964A patent/HUT63851A/en unknown
- 1991-09-27 JP JP3516127A patent/JPH06501261A/en active Pending
- 1991-09-27 WO PCT/SE1991/000653 patent/WO1992006102A1/en not_active Application Discontinuation
- 1991-09-27 AU AU86410/91A patent/AU8641091A/en not_active Abandoned
- 1991-09-27 US US08/030,168 patent/US5473063A/en not_active Expired - Fee Related
- 1991-09-27 CA CA002093020A patent/CA2093020A1/en not_active Abandoned
- 1991-10-01 PT PT99130A patent/PT99130A/en not_active Application Discontinuation
-
1995
- 1995-08-18 US US08/516,551 patent/US5747473A/en not_active Expired - Fee Related
-
1998
- 1998-03-12 US US09/041,135 patent/US5952500A/en not_active Expired - Fee Related
-
1999
- 1999-06-17 US US09/334,756 patent/US6121431A/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP0554274A1 (en) | 1993-08-11 |
| AU8641091A (en) | 1992-04-28 |
| US6121431A (en) | 2000-09-19 |
| JPH06501261A (en) | 1994-02-10 |
| US5473063A (en) | 1995-12-05 |
| HUT63851A (en) | 1993-10-28 |
| PT99130A (en) | 1992-08-31 |
| US5747473A (en) | 1998-05-05 |
| US5952500A (en) | 1999-09-14 |
| SE9003151D0 (en) | 1990-10-02 |
| IE913374A1 (en) | 1992-04-08 |
| KR930702371A (en) | 1993-09-08 |
| WO1992006102A1 (en) | 1992-04-16 |
| HU9300964D0 (en) | 1993-06-28 |
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