CA2114788C - Synthetic storage proteins with defined structure containing programmable levels of essential amino acids for improvement of the nutritional value of plants - Google Patents

Abstract

There is provided synthetic nucleic acid fragments for the altered expression of selected nutritionally-important proteins in plants. These nucleic acid fragments may be used to transform plants, particularly crop plants, to increase the lysine and methionine content of seeds or leaves. The invention is of significant interest for the nutritional improvement of corn which is low in lysine and sulfur amino acid-poor plants, such as corn and soybean. Thre is also provided chimeric genes, host cells, plants, seeds and microorganisms containing the nucleic acid fragment as well as methods for obtaining the expression of particular proteins in plants and microorganisms.

Description

WO 93/0316a PCT/US92/06412 ~~. i~'~~8 SYNTHETIC STORAGE PROTEINS WITH DEFINED STRUCTURE
CONTAINING PROGRAMMABLE LEVELS OF ESSENTIAL AMINO ACIDS
FOR IMPROVEMENT OF THE NUTRITIONAL VALUE OF PLANTS
BACKGROUND OF THE INVENTION
The worldwide animal feed market, which includes livestock, poultry, aquaculture and pets is 975 million metric tons. In the United States 180 million metric tons are consumed, with corn (~ mavs L.) accounting for about 67% and soybean (Glycine ~ L.) meal for about 10% of the total. Corn and soybean prodUCts are also a mayor element of international trade.
Human food and animal feed derived from many grains are deficient in some of the ten essential amino acids (cysteine, iso~leucine, lysine, methionine, phenylalanine, threonine, tyrosine, and valine) which are required in animal diets. In corn; lysine is the most limiting amino acid followed by tryptophan and the sulfur amino acids, methionine and cysteine, for the dietary requirements of many animals. The u,~efulness of soybean meal, which is rich in lysine and tryptophan, to supplement corn in animal feed is limited by the low sulfur amino acid content of the legume. When soybean meal is used to supplement the lysine levels of corn, the low levels of methionine in soybeans cause the blended feed to have an even lower level of methionine than the original corn. As a result, feed blends of corn and soybean typically still include methionine as an additive. A typical composition of chicken starter rations is shown in Table 1 (Powell et al., (1976) Poult. Science. 55:502-509].

~.1~~~ ~~'~ ~ 2 Table 1 Composition of Practical Chicken Starer Rations Yellow Corn 57.25%

Soybean Meal (49% protein) 29.00 .

Fish solubles 0.65 Wheat middlings 2.50 Delactosed whey 1.50 Costal bermudagrass, dehydrated 5.00 Minerals 0.25 Vitamins 0.25 Animal fat 0.25 DL-Methionine 0.10 Choline chloride 0.10 Thus, a mechanism to increase the levels of particular amino acids within the plant seed for a given crop and a specific end use would eliminate the need to supplement mixed or single grain feeds. with purified amino acids.
Furthermore, the methionine requirements of poultry and swine (the two largest consumers of soybean meal accounting for 78% of the soy protein used .in feeds [Wilcox, (198?) Agronomy 16:823]) decrease with age of the animal [Ensminger et al., (1978) Feeds and , Nutrition, The Ensminger Publishing Co. Clovis, CA.].
The ability to improve the essential amino acid content of soybean or corn in a controllable manner is therefore, extremely desirable. A solution to this problem is the design of a class of synthetic proteins which can be tailored to complement the deficiencies of any crop for use in feeding any animal of any age.
Ttie amino acid content of seeds is determined primarily by the storage proteins which are synthesized during seed development and which serve as a major nutrient reserve following germination. The quantity of protein in seeds varies from about 10% of the dry weight WO 93/03160 ~ ~ ~ /~ ~ ~ ~ PCT/US92/06412 in cereals to 20-40% of the dry weight of legumes. In many seeds the storage proteins account for 50% or more of the total protein. Because of their abundance, plant seed storage proteins were among the first plant proteins to be isolated. Only recently, however, have the amino acid sequences of some of these proteins been determined with the use of molecular genetic techniques.
These techniques have also provided information about the genetic signals that control the seed-specific expression and the intracellular targetting of these proteins.
Although no plant seed storage proteins enriched in lysine relative to average lysine content of plant proteins have been identified, a number of sulfur-rich plant seed storage proteins have been identified and their corresponding genes isolated. A gene in corn for a 15 kD zein protein containing 11% methionine and 5%
cysteine [Pedersen et al., (1986) J. Biol. Chem.
261:6279-6284] and a gene for a 20 kD zein protein containing 23% methionine and 3% cysteine have been isolated [Kirihara et al., (1988) Mol. Gen..Genet.
21:477-489; Kirihara et al., (1988) Gene 71:359-370].
Two genes from pea for seed albumins containing 8% and , 16% cysteine have been isolated [Higgins et al., (1986) J. Biol. Chem. 261:11124-11130]. A gene from Brazil nut for a 28 albumin containing 18% methionine and 8%
cysteine has been isolated [Altenbach et al., (1987) Plant Mol. Biol. 8:239-250]. Finally, a gene coding for a 10 kD seed prolamin containing 19% methionine and 10%
cysteine has been isolated from rice [Masumura et al., (1989) Plant Mol. Biol. 12:123-130].
Plant breeders have long been interested in using naturally-occuring variations to improve protein quality and quantity in crop plants [Deutscher, (I978) Adv. Exp.
Medicine and Biology 105:281-300]. Maize lines ..~
~:,~~'~ li~~
containing higher levels of lysine (70% increase) and tryptophan (100% increase) have been identified [hertz, (1964) Science 145:279 and Nelson, (1965) Science 150:1469-70]. However, these lines which incorporate a .
mutant gene, opaque-2, exhibit poor agronomic qualities (increased susceptibility to disease and pests, 8-19%
reduction in yield, low kernel weight, slower drying, lower dry milling yield of flaking grits, and increased storage problems) and are not commercially useful [Deutscher, (1978) Adv. Exp. Medicine and Biology 105:281-300]. Further breeding to improve the agronomics of opaque-2 lines is complicated because several modifier genes are involved which have complex inheritence patterns [Vasal, S. K. (1974) Symposium Proceedings. Worldwide Maize Improvement in the 70's and the Role for CIMMYT. Centro Internacional de Mejoramiento de Maiz y Trigo. E1 Batan, Mexico]. In spite of the difficulties, a few researchers have continued to work with opaque-2 mutants. Quality Protein Maize (QPM), bred at CIMMYT using the opaque-2 and sugary-2 genes and associated modifiers,, has a hard endosperm and enriched levels of lysine and tryptophan in the kernals [Vasal et al., Proceedings of the 3rd , Seed Protein Symposium, Gatersleben, August 31-September 2, (1983)].
However, the gene pools represented in the QPM
lines are tropical and subtropical and they are only available as open pollinated types (hybrids are mainly used in the United States) [National Research Council Report (1988) Quality Protein Maize. National Academy Press, Washington, D.C.]. QPM is genetically complex and the existing lines are not easily adapted to the dent germplasm used in the United States. These factors .
prevent the adoption of QPM by U.S. corn breeders.

WO 93/03160 ~ ~ '1 l1 r~ ~ ~ PCT/US92/06412 S
Efforts to improve the sulfur amino acid content of crops through traditional plait breeding have met with limited success on the laboratory scale and no success on the commercial scale. A mutant corn line with an S elevated whole-kernel methionine concentration was isolated from corn cells grown in culture by selecting for growth in the presence of inhibitory concentrations of lysine plus threonine [Phillips et al., (1985) Cereal Chem. 62:213-218]. However, agronomically-acceptable cultivars have not yet been derived from this line.
Traditional breeding efforts designed to increase the level of methionine in soybean protein have been limited compared to efforts to increase overall protein quantity in soybeans [Burton, 1984 World Soybean Research Conference IrI Proceedings, p. 361-368 (August 12-August 17, 1984)]. Soybean cell lines with increased intracellular concentrations of methionine were isolated by selection for growth in the presence of ethionine, a nonmetabolizable methionine analog, [Madison et al., (1988) Plant Cell Reports 7:472-476], but plants were not regenerated from these lines.
Recombinant DNA technology offers the potential for altering the amino acid composition of crop plants.
Particularly useful technologies are: (a) methods for the molecular cloning and ,iH, vitro manipulation of genes [see Sambrook et~al., (1989) Molecular Cloning: a Laboratory Manual, 2nd Ed., Cold Spring Harbor Laboratory Press], (b) introduction of genes via transformation into agriculturally-important crop plants such as soybean [Chas et al., (1989) Plant Physiol.
91:1212-1218: Christou et al., (1989) Proc. Nat. Acad.
Sci I1.S.A. 86:7500-7504; Hinchee et al., (1989) Biotechnology 6:915-922; EPO publication 0301 749 A2], rapeseed [De Block et al., (1989) Plant Physiol.
91:694-701], and corn iGordon-Kamm et al., (1990) Plant ~~~~~ l~i~ 6 Cell 2:603-618; Fromm et al., (1990) Biotechnology 8:833-839], and (c) seed-specific expression of introduced genes in transgenic plants [see Goldberg et al., (1989) Cell 56:149-160); Thompson et al., (1989) .
BioEssays 10:108-113]. In order to use these technologies to develop crop plants with increased lysine and sulfur amino acid content, it is essential to obtain or develop commercially-important gene products enriched in the appropriate amino acids.
Expression of seed storage protein genes in transgenic plants have been reported in the model plant systems, tobacco or petunia, because it has only recently become possible to transform agriculturally-important crop plants such as corn and soybean. In general, divot seed storage protein genes were expressed in a seed-specific manner in transformed divot plants.
Furthermore, both tempora~ and spatial control of gene expression was maintained. The transgenic protein products have, in some cases, been shown to be correctly processed and assembled into appropriate multimeric forms. [Beachy et al., (1985) EMBO J. 4:3047-3053;
Sengupta-Gopalan et al., (1985) Proc. Natl. Acad. Sci.
USA 82:3320-3324 Barker et al., (1988) Proc. Natl.
Acad. Sci. USA 85:458-462; Ellis et al., (1988) Plant Mol. Biol. 10:203-219; Naito et al., (1988) Plant Mol.
Biol. 11:109-123; iioffman et al., (1988) Plant Mol.
Biol. 11:717-729; Altenbach et al., (1989) Plant Mol.
Biol. 13:513-522].
Storage proteins are usually targetted to subceliular locales by the processing of N-terminal signal peptides or carboxy-terminal sequences such as SEKDE1, [Bednareket al., (1990) The Plant Cell, 2:1145-1155. Munro et al., (1987) Cell 48:899-907; Pelham, (1988) EMBO J. 7:913-918: Pelham et al., (1988) EMBO J.
7:1757-1762: Inohara et al., (1989) Proc. Natl. Acad.

~:~1~'~~~
Sci. U.S.A. 86:3569-3568; Hesse et al., (1989) EMBO J.
8:2953-2461.]. It may prove necessary to create chimeric genes incorporating these signals for proper localization and stability of synthetic storage S proteins.
Expression of seed storage protein genes in the leaves of plants is accomplished by replacing the regulatory signals that function in the seed with signals that function in the leaf [Lawton et al., (1987) Plant Mol. Biol. 9:315-324; Schernthaner et al., (1988) EMBO J. 7:1249-1255]. Monocot seed storage protein genes were expressed at very low levels [Schernthaner et al., (1988) EMBO J. 7:1249-1255] and, in one case, expressed in a non-seed-specific manner in transformed divot plants [Ueng et al., (1988) Plant Physiol.
86:1281-1285]. Replacement of the monocot regulatory regions (promoter and transcription terminator) with divot seed-specific regulatory regions resulted in low level seed-specific expression of the protein in one case [Williamson et al., (2988) Plant Physiol.
88:1002-1007]. In another case, high-level"seed-specific expression of the monocot protein was found and the signal sequence of the monocot precursor was also correctly processed [Hoffman et al., (1987) EMHO J.
2S 6:3213-3221].
In order to increase the lysine and sulfur amino acid contents of seeds, it is essential to obtain a gene or genes coding for a protein rich in lysine and the sulfur-containing amino acids methionine and cysteine.
Methionine is preferable to cysteine because methionine can be converted to cysteine by most animals, while cysteine cannot be converted to methionine. It is desirable that the introduced protein be compatible with the target crop plant. It is desirable to select the gene to maximise lysine and sulfur amino acid content WO 93!03160 PCT/US92/06412 ~,~1'.~~f ~~
thereby minimizing the level of expression required in the plant to satisfy end-user needs. For this reason, those skilled in the art have not restricted themselves to natural genes and their polypeptide products. v Jaynes et al. worked with synthetic polypeptides which have elevated levels of lysine, methionine, tryptophan, threonine, and isoleucine as compared to known proteins [WO 89/04371]. These synthetic genes Were formed by random ligation of mixtures of small oligodeoxy-nucleotides containing a high proportian (25-60%) of codons for essential amino acids. The proteins so formed are heterogeneous and do not fold to defined structures. Limited expression (0.02 - 0.35% of total plant protein) has been demonstrated in potato [Yang et al., (1989) Plant Science, 64:99-111].
Others have modified natural proteins by addition or replacement of amino acids to increase the lysine or methionine content [DeClercq et al., EP 0 318 341 A1].
DeClercq et al. have shown that it is possible to express modified storage protein genes in tobacco, and ~,~ plants . However, their work gives little guidance regarding the design of the sequences rich in appropriate amino acids which are to , be inserted into a target gene product. They observe only that the stability of the molecule should not be influenced and that long stretches of methionines should be interrupted by amino acids which break helical structures. Furthermore, the specific polypeptide sequences inserted into the target gene were not designed to adopt uniquely defined stable structures.
The importance of gene pr~duct stability in the .
seed dictates the need for a polypeptide of defined structure, while the need to complement existing amino acid composition and to satisfy end-user requirements emphasizes the importance of a flexible system which can WO 93/031b0 PCT/US92/06412 ~~:~~'~$8 s accomodate variations in composition without sacrificing final gene product stability.
~UN~~ARY OF THF, INVENTION
Applicants have provided an invention which overcomes the limitations of naturally-occurring plant proteins and provides flexibility to satisfy the nutritional requirements of humans and animals by means of the design and expression in plants and microorganisms of synthetic seed storage proteins (SSPs) .
The introduction into transgenic crop plants of a chimeric gene comprising seed storage protein regulatory sequences and an appropriate protein coding sequence rich in essential amino acids represents an approach to improve the nutritional quality of seeds from crop plants. These sequences can be designed to code fox amino acid sequences which result in a particular tertiary structure that improves stablity. The increase in essential amino acid content of the seed will be c~termined by: (a) the level of expression of the chimeric gene in the transformed crop, which depends, in part, upon the seed-specific expression, the translatability and stability of the mRNA and targetting~
signals used; (b) the percentage of the designated amino acid residues in the seed storage protein coding region;
(c) the stability of the introduced protein i:9~ the seed of the transformed crop plant, which depends, in part, upon its proper processing, intracellular targetting, assembly into higher-order structures, and ability to withstand desiccation; and (d) the compatibility of the introduced protein with the native seed proteins of the transformed crop.
The present invention provides a means to overexpress proteins high in lysine and methionine content by providing gene sequenceQ and promoters ~~.1' ~'C~
'~. ~ ('.' c,) 10 capable of transforming target plants. Specifically one aspect of the present invention is a synthetic polypeptide comprising n heptad units td a f g a b c), each heptad being either the same or different, wherein: .
n is at least 4;
a and d are independently selected from the , group consisting of Met, Leu, Val, Ile and Thr;
a and g are independently selected from the group consisting of the acid/base pairs Fr:
Glu/Lys,'Lys/Glu, Arg/Glu, Arg/Asp, Lys/Asp, Glu/Arg, Asp/Arg and Asp/Lys; and b, c and f are independently any amino acids except Gly or Pro and at least two amino acids of b, c and f in each heptad are selected from the group consisting of Glu, LyB, ASp, Arg, HiB, Thr, Ser, ASn, Ala, Gln, Cys and Ala.
Additionally, this aspect of the invention may have a + d independently selected from the group consisting of Met and Leu, or where a and g are independently either Lys/Glu or Glu/Lys, or where b, c, and f are selected such that if f is a charged amino acid then b or c carries the opposite charge.
In addition, another aspect of the invention further obeys conditions whereins n is at least 4;
a and d are independently selected from the group consisting of Met and Leu;
a and g are independently either LyslGlu or Glu/Lys: and .
b, c and f are independently any amino acids except Gly or Pro, at least two amino acids - -of b, c, and f in each heptad are selected from the group Glu, Lys, Asp, Arg, His, Thr, WO 93103160 ~ PCT/US92/06412 _ ~.~:~~'~$8 Ser, Asn, Ala, Glu, and Cys, and b, c, and f are selected such that if f is a charged amino acid then b or c carries the opposite charge.
Specific heptad units which conform to the invention are:
LEEKLKA (SEQ ID N0:60) LKEELKA (SEQ ID N0:69) MEEKLKA (SEQ ID N0:61) MKEELKA (SEQ ID N0:70) MEEKMKA (SEQ ID N0:62) MKEEMKA (SEQ ID N0:71) LEEKLKK (SEQ ID N0:63) LKEELKK (SEQ ID N0:72) MEEKLKK (SEQ ID N0:64) MKEELKK (SEQ ID N0:73) MEEKMKK (SEQ ID N0:65) r~CEEMKK (SEQ ID N0:74) LEEKLKW (SEQ ID N0:66) LKEELKW (SEQ ID N0:75) MEEKLKW ( SEQ ID NO : 67 ) I~aCEELKW ( SEQ ID NO : 7 6 ) MEEKN~CW (SEQ ID N0:68) MKEEMKW (SEQ ID N0:77) MEDIC1~CW ( SEQ ID NO : 7 8 ) LEEKMKV ( SEQ ID NO : 81 ) LKEEMAK (SEQ ID N0:79) r~CDEMWK (SEQ ID N0:82) LKEENgCIC (SEQ ID N0:80) A further aspect of the invention are synthetic polypeptides selected from the group consisting of SEQ
ID NOS : 1, 2, 3, 4, 5, 6, 7 and 83 .
An aspect of the invention are the nucleic acid fragments encoding the claimed polypeptides.

Another aspect of the invention is a chimeric gene, comprising a regulatory sequence of the invention and a DNA sequence coding~for the desired protein operably linked to the regulatory sequence such that the transformed host cell expresses the desired protein.
When the preferred host cell is eukaryotic and selected from corn, soybean, ~rassic~ spp. (~. naD'IIS, istr3s, ~. oleracea), tobacco, rice, potato, forage grasses, and Wheat, preferred regulatory sequences are those active in plant seeds and include those encoding for soybean kunitz trypsin inhibitor, glycinin, ~-eonglycinin, lectin, bean lectin, phaseolin, corn 10 WO 93/03160 PC'T/US92/06412 ~11~'lg8 kD zein, 27 kD zein, and 19 kD zein globulin 1 and globulin 2. When the preferred host cell is ~. rat; the preferred regulatory sequence is a bacteriophage T7 promoter system and translational initiation sequence.
A further aspect of the invention is a host cell transformed with the chimeric gene of the invention such that the transformed host cell expresses the desired protein. Yet another aspect of the invention is a plant transformed with the chimeric gene of the invention such that the transformed plant expresses the desired protein. The seeds of such transformed plants are also regarded as an embodiment of the invention.
A final aspect of the invention is a method of varying the content of essential amino acids in plants in response to end-user nutritional requirements, comprising the steps of a. assessing deficiencies of a feed crop plant relative to the end-user nutritional requirements:
b. synthesizing a nucleic acid fragment that encodes a polypeptide, for instance, of the structure described herein, to correct the amino acid deficiencies identified in step a;
c. combining the nucleic acid synthesized i~
step b with regulatory sequences for expression and localization in plant tissues;
d. transforming a plant cell with the product of step c;
e. regenerating plants from said transformed plant cell of step d to obtain mature plants;
f. screening the seeds of the plants of step a for the desired variation in amino acid level. ' The invention can be more fully understood from the following detailed description, the accompanying drawings and the Sequence Descriptions which form a part of this application. The Sequence Descriptions contain the one letter code for nucleotide sequence characters and the three letter codes for amino acids in conformity with the IuPAC-lUB standards described in Nucleic Acids Research 13:3021-3030 (1985) and in The Biochemical 5 Journal 219:345-373 (1984) which are incorporated by reference herein. The amino acid residues are labelled according to the conventions of the coiled-coil literature. The citation herein of any patents, pending U.S. applications, and any other disclosure that was 10 available to the public as of the filing date of the instant application may be referred to in their entirety.
BRIEF DESCRIPTION OF THE DRAWINGS
AND SE
15 Figure 1 shows an alpha helix from the side and top views.
Figure 2 shows end (Figure 2a) and side (Figure 2b) views of an alpha helical coiled-coil structure.
Figure 3 shows the chemical structure of leucine 20 and methionine emphasizing their similar shapes.
Figure 4 shows end views of the two sets of coiled-coil polypeptides which were chemically synthesized and characterized. The CSP series are shown in Figure 9a and the SSP series are shown in Figure 4b.
25 Figure 5 depicts the strategy for creating a vector (ASKS) for use in construction and expression of the SSP
gene sequences.
Figure 6 shows the strategy for inserting oligonucleotide sequences into the unique Earl site of 30 the base gene sequence.
Figure 7 shows the insertion of the base gene oligonucleotides into the NcoIlEcoRI sites of pSKS to create the plasmid pSK6. This base gene sequence was used as in Figure 6 to insert the various SSP coding WO 93/03160 PCf/US92/06412 regions at the unique Earl site to create the cloned segments listed.
Figure 8 shows the insertion of the 63 by "segment"
oligonucleotides used to create non-repetitive gene sequences for use in the duplication scheme in Figure 9.
Figure 9 (a + b) shows the strategy for multiplying non-repetitive gene "segments" utilizing in-frame fusions.
Figure 10 shows the construction of a chimeric gene for expression of SSPs in plants.
Figure 11 shows the vectors containing seed specific promoter and 3' sequence cassettes. SSP
sequences were inserted into these vectors using t:he Ncol and Asp718 sites as in Figure 10.
Figure 12 shows the transfer of the chimeric SSP
genes to the binary vector pZS97 or pZS97K for use in ~ transformation of plant tissue.
Figure 13 depicts the construct used for expression of SSP-3-5 protein in rice protoplast. HPH 508 promoter is a chemically inducible promotes derived from corn.
The SEQ ID NOS:1-7 and 83 are SSP polypeptide sequences suitable for expression ~,p,viva.
The SEQ ID NOS:8 and 3i are CSP polygeptide , sequences unsuitable for expression ~, vivo.
The SEQ ID NOS:60-82 are specific claimed embodiments of SSP polypeptide sequences suitable for expression ~,n, y,i,y~.
The SEQ ID NOS:9-3Q, and 32-59, 84-105, 110-112 represent nucleic acid fragments and the proteins they encode referenced in the text and used in the development of the claimed invention.
DEFTN'~TTpN, In the context of this disclosure, a number of terms shall be utilized. As used herein, the term !'nucleic acid" refers to a large molecule which can be WO 93/03160 ~ ~ ~ ~ ~ ~ ~ PCT/US92/06412 single-stranded or double-stranded, composed of monomers (nucleotides) containing a sugar, phosphate, and either a purine or pyrimidine. A "nucleic acid fragment" is a fraction of a given nucleic acid molecule. In higher 5 plants, deoxyribonucleic acid (DNA) is the genetic material while ribonucleic acid (RNA) is involved in the transfer of the information in DNA into proteins. A
"genome" is the entire body of genetic material contained in each cell of an organism. The term 10 "nucleotide sequence" refers to a polymer of DNA or RNA
which can be single- or double-stranded, optionally containing synthetic, non-natural or altered nucleotide bases capable of incorporation into DNA or RNA polymers.
As used herein, the term "homologous to" refers to 15 the complementarity between the nucleotide sequence of two nucleic acid molecules or between the amino acid sequences of two protein molecules. Estimates of such homology are provided by either DNA-DNA or DNA-RNA
hybridization under conditions of stringency as is well understood by those skilled in the art [as described in Hames and Higgins, Eds.. (1985) Nucleic Acid Hybridisation, IRL Press, Oxford, U.K.]; or by the comparison of .sequence similarity between two nucleic acids or proteins.
2S As used herein, "substantially homologous" refers to nucleic acid molecules which require less stringent conditions of hybridization than those for homologous sequences, and also refers to coding DNA sequences which may involve base changes that do not cause a change in the encoded amino acid, or which involve base changes which may alter one or more amino acids, but not affect the functional properties of the protein encoded by the DNA sequence. Thus, the nucleic acid fragments described herein include molecules Which comprise 3S possible variations of the nucleotide bases derived from rl a ~ , ..

deletion, rearrangement, random or controlled mutagenesis of the nucleic acid fragment, and even occasional nucleotide sequencing errors so long as the DNA sequences are substantially homologous.
"Gene" refers to a nucleic acid fragment that expresses a specific protein, including regulatory .
sequences preceding (5' non-coding) and following (3' non-coding) the coding region. "Native" gene refers to the gene as found in nature with its own regulatory sequences. "Chimeric" gene refers. to a gene comprising heterogeneous regulatory and coding sequences. A
"foreign" gene refers to a gene not normally found in the host organism but that is introduced by gene transfer.
"Coding sequence" refers to a DNA sequence that codes for a specific protein and excludes the non-coding sequences. It may constitute an "uninterrupted coding sequence", i.e., lacking an intron, such as in a cDNA or it may include one or more introns bounded by appropriate splice junctions. An "intron" is a sequence of RNA which is transcribed in the primary transcript but which is removed through cleavage and re-ligation of the RNA within the cell to create the mature mRNA that , can be translated into a protein.
"Initiation colon" and "termination colon" refer to unit of three adjacent nucleotides in a coding sequence that specifies initiation and chain termination, respectively, of protein synthesis (mRNA
translation). "Open reading frame" refers to the amino acid sequence encoded between translation initiation and termination colons of a coding sequence.
"RNA transcript" refers to the product resulting from RNA polymerase-catalyzed transcription of a DNA -sequence. When the RNA transcript is a perfect complementary copy of the DNA sequence, it is referred 211 ~'~~~
to as the primary transcript or it may be a RNA sequence derived from posttranscriptional processing of the primary transcript and is referred to as the mature RNA.
"Messenger RNA" (mRNA) refers to the RNA that is without S introns and that can be translated into protein by the cell. w As used herein, "suitable regulatory sequences"
refer to nucleotide sequences located upstream (5'), within, and/or downstream (3') to a coding sequence, which control the transcription and/or expression of the coding sequences, potentially in conjunction with the protein biosynthetic apparatus of the cell. "Suitable regulatory sequences" include promoters, enhancers, translation leader sequence, 3' non-coding sequence.
"Promoter" refers to a DNA sequence in a gene, usually upstream (5') to its coding sequence, which controls the expression of the coding sequence by providing the recognition for RNA polymerase and other factors required for proper transcription. A promoter may also contain DNA sequences that are involved in the binding of protein factors which control the effectiveness of transcription initiation in response to physiological or developmental conditions. It may also .
contain enhancer elements.
An "enhancer" is a DNA sequence which can stimulate promoter activity. It may be an innate element of the promoter or a heterologous element inserted to enhance the level and/or tissue-specificity of a promoter.
"Constitutive promoters" refers to those that direct gene expression in all tissues and at all times.
"Organ-specific" or "development-specific" promoters as referred to herein are those that direct gene expression almost exclusively in specific organs, such as leaves or seeds, or at specific development stages in an organ, such as in early or late embryogenesis, respectively.

WO 93/03160 ~ PGT/US92/06412 ~11~'l~~

The term "expression", as used herein, is intended to mean the production of the protein product encoded by a gene. A "gene product" refers to the protein produced by the translation of an mRNA corresponding to a gene sequence. "Overexpression" refers to the production of a gene product in transgenic organisms that exceeds .
levels of production of that protein in normal or non-transformed organisms.
The "3' non-coding sequences" refers to the DNA
sequence portion of a gene that contains a transcription termination signal, a polyadenylation signal and any other regulatory signal capable of affecting mRNA
processing or gene expression. The polyadenylation signal is usually characterized by affecting the addition of polyadenylic acid tracts to the 3' end of the mRNA precursor.
The "translation leader sequence" refers to that DNA sequence portion of a gene between the promoter and coding sequence that is transcribed into RNA and is present in the fully processed mRNA upstream (5') of the translation start codon. The translation leader sequence may affect processing of the primary transcript to mRNA, mRNA stability or translation efficiency. , "Signal peptide" refers to the amino terminal extension of a polypeptide, which is translated in conjunction With the polypeptide forming a precursor peptide and which is required for its entrance into the secretory pathway. The term "signal sequence" refers to a nucleotide sequence that encodes the signal peptide.
"Intracellular localization sequence" refers to a nucleotide sequence that encodes an intracellular targetting signal. An "intracellular targetting signals' is an amino acid sequence which is translated in -conjunction with a protein and directs it to a particular sub-cellular compartment. "Endoplasmic WO 93/03160 ~ ~'~ $ ~ PCT/US92/Ob412 reticulum (ER) stop transit signal" refers to a carboxy-terminal extension of a polypeptide, which is translated in conjunction with the polypeptide and causes a protein that enters the secretory pathway to be retained in the ER. "ER stop transit sequence" refers to a nucleotide sequence that encodes the ER targetting signal. Other intracellular targetting sequences encode targetting signals active in seeds and/or leaves and vacuolar targetting signals.
"Transformation" herein refers to the transfex of a foreign gene into the genome of a host organism and its genetically stable inheritance. Examples of methods of plant transformation include ggtQ~-mediated transformation and particle-accelerated or "gene gun"
transformation technology. "Forage grasses" herein refer to grasses utilized as fodder for animals. "Host cell" refers to a plant, animal or microorganism cell transformed with the chimeric gene of this invention.
"Amino acids" herein refer to the naturally occuring L amino acids (Alanine, Arginine, Aspartic acid, Asparagine, Cystine, Glutamic acid, Glutamine, Glycine, Histidine, Isoleucine, Leucine, Lysine, Methionine, Proline, Phenylalanine, Serine, Threonine, Tryptophan, Tyrosine, and Valine). "Essential amino acids" are those amino acids which cannot be synthesized by animals. A "polypeptide" or "protein" as used herein refers to a molecule composed of monomers (amino acids) linearly linked by amide bonds (also known as peptide bonds ) .
"Synthetic protein" herein refers to a protein consisting of amino acid sequences that are not known to occur in nature. The amino acid sequence may be derived from a consensus of naturally occuring proteins or may be entirely novel.

"Primary sequence" refers to the connectivity order of amino acids in a polypeptide chain without regard to the conformation of the molecule. Primary sequences are written from the amino terminus to the carboxy terminus .
5 of the polypeptide chain by convention.
"Secondary structure°' herein refers to physico-chemically favored regular backbone arrangements of a polypeptide chain without regard to variations in side chain identities or conformations. °'Alpha helices" as 10 used herein refer to right-handed helices with approximately 3.6 residues residues per turn of the helix. An "amphipathic helix" refers herein to a polypeptide in a helical conformation where one side of the helix is predominantly hydrophobic and the other 15 side is predominantly hydrophilic.
"Coiled-coil" herein refers to an aggregate of two parallel right-handed alpha helices which are wound around each other to form a left-handed superhelix.
"Salt bridges" as discussed here refer to acid-base 20 pairs of charged amino acid side chains so arranged in space that an attractive electrostatic interaction is maintained between two parts of a polypeptide chain or between one chain and another.
"Host eell" means the cell, either plant or animal, that is transformed with the introduced genetic material.
In the context of this invention a protein °°enriched" in an essential amino acid is one which contains a higher percentage of that amino acid than is found fox average mixtures of natural proteins.
p~, ~rzor~
design of SSP goZvoegtide Senuences One aspect of this invention is the design of polypeptides which can be expressed ~ yiyQ to serve as synthetic seed storage proteins. Polypeptides are WO 93/03160 ' '' ~ ' PCT/US92/06412 211: l ~

linear polymers of amino acids where the a-carboxyl group of one amino acid is covalently bound to the OC-amino group of the next amino acid in the chain. Non-covalent interactions among the residues in the chain and With the surrounding solvent determine the final conformation of the molecule. Those skilled in the art must consider electrostatic forces, hydrogen bonds, Van der Waals forces, hydrophobic interactions, and conformational preferences of individual amino acid residues in the design of a stable folded polypeptide chain (see for example: Creighton, (1984) Proteins, Structures and Molecular Properties, W. H. Freeman and Company, New York, pp. 133-197., or Schulz et al., (1979) Principles of Protein Structure, Springer Verlag, New York, pp. 27-45]. The number of interactions and their complexity suggest that the design process may be aided by the use of natural protein models Where possible.
The synthetic storage proteins (SSPs) embodied in this invention are chosen to be polypeptides With the potential to be enriched in lysine, methionine, or tryptophan relative to average levels (see Table 2) (Schulz et al., (1979) Principles of Protein Structure.
Springer Verlag, New York, pp.2; Bright et al., (1983) CRC Critical Rev. Plant Sci. 1:49-93; and Smith et al., Eds., (1978) Soybeans: Chemistry and Technology. Vol 1:
The AV1 Publishing Co. Westport, CT].
Table 2 gv eraqe no Acid t of Protein Ami ~~nten DerivPid , or. Plan From Bact~_ri_a E. coli Corn Soybean SSP s Lysine 7.0 3.52 6.4 up to 430 Methionine 3.8 2.04 1.6 up to 430 Tryptophan 1.0 0.94 1.2 up to 140 ~~~t~ ~~t7~ 22 Lysine is a charged amino acid at physiological pH
and is therefore found most often on the surface of protein molecules [Chotia, (1976) Journal of Molecular Biology 105:1-14]. To maximize lysine content Applicants chose a molecular shape with a high surface-to-volume ratio for the synthetic storage proteins embodied in this invention. The alternatives were either to stretch the common globular shape of most proteins to form a rod-like extended structure or to flatten the globular shape to a disk-like structure.
Applicants chose the former configuration as there are several natural models for long rod-like proteins in the class of fibrous proteins [Creighton, (1984) Proteins, Structures and Molecular Properties, W.H. Freeman and Company, New York, p.191].
Coiled-coils constitute a well-studied subset of the class of fibrous proteins [see Cohen et al., (1986) Trends Biochem. Sci. 11:245-248]. Natural examples are found in a-keratins, paramyosin, light meromyosin and tropomyosin. These protein molecules consist of two parallel alpha helices twisted about each other in a left-handed supercoil. The repeat distance of this supercoil is 140 .~ (compared to a repeat distance of 5.Q
~ for one turn of the individual helices). The supercoil causes a slight skew (10°) between the axes of the two individual alpha helices.
In a coiled coil there are 3.5 residues per turn of the individual helices resulting in an exact 7 residue periodicity with respect to the superhelix axis (see Figure 1). Every seventh amino acid in the polypeptide chain therefore occupies an equivalent position with respect to the helix axis. Applicants refer to the seven positions in this heptad unit of the invention as (d a f g a b c) as shown in Figures 1 and 2a. This ~~~ L~'~88 conforms to the conventions used in the coiled-coil literature.
The a and d amino acids of the heptad follow a 4,3 repeat pattern in the primary sequence and fall on one S side of an individual alpha helix (See Figure 1). If the amino acids on one side of an.alpha helix are all non-polar, that face of the helix is hydrophobic and will associate with other hydrophobic surfaces as, for example, the non-polar face of another similar helix. A
coiled-coil structure results When two helices dimerize such that their hydrophobic faces are aligned with each other (See Figure 2a).
The amino acids on the external faces of the component alpha helices (b, c, e, f, g) are usually polar in natural coiled-coils in accordance with the expected pattern of exposed and buried residue types in globular proteins [Schulz, et al., (1979) Principles of . Protein Structure. Springer Verlag, New York, p. 12;
Talbot, et al , (1982) Acc. Chem. Ftes. 15:224-230;
Hodges et al., (1981) Journal of Biological Chemistry 256:1214-1224]. Charged amino acids are sometimes found forming salt bridges between positions a and g' or positions g and e' on the opposing chain (see Figure 2a) .
Thus; two amphipathic helices like the one shown in Figure 1 are held together by a combination of hydrophobic interactions between the a, a', d, and d' residues and by salt bridges between a and g' and/or g and e' residues. The packing of the hydrophobic residues in the supercoil maintains the chains "in register". For short polypeptides comprising only a few turns of the component alpha helical chains, the 10°
skew between the helix axes can be ignored and the two chains treated as parallel (as shown in Figure 2a).

~,1 ~. ~ 'l 2 9 A number of synthetic coiled-coils have been reported in the literature (Lau et al., (1984) Journal of Biological Chemistry 259:13253-13261; Hodges et al., (1988) Peptide Research 1:19-30: DeGrado et al., (1989) .
Science 243:622-628; O'Neil et al., (1990) Science 250:646-651]. Although these polypeptides vary in size, Lau et al. found that 29 amino acids were.sufficient for dimerization to form the coiled-coil structure (Lau et al., (1984) Journal of Biological Chemistry 259:13253-13261. Applicants constructed the polypeptides in this invention as 28-residue and larger chains for reasons of conformational stability.
A class of DNA binding proteins known as leucine sippers contain regions (usually 28 to 35 residues in length) which form a coiled-coil structure resulting in assembly of the protein into dimeric structures (0'Shea et al., (1989) Science 243:538. Sequence analysis ~f these proteins shows that the d position is almost always occupied by a leucine residue. This position is also preferentially occupied by leucine in tropomyosin (Bodges et al., (1981) J. Biol. Chem. 256:1219-1224].
r The eight e, g, e', and g' positions in these leucine zipper sequences are often charged residues. However natural protein sequences rarely form more than two interhelix salt bridges in one molecule.
The polypeptides of this invention are designed to dimerize with a coiled-coil motif in aqueous environments. Applicants have used a combination of hydrophobic interactions and electrostatic interactions to stabilize the coiled-coil conformation. lMost nonpolar residues are restricted to the a and d positions which creates a hydrophobic stripe parallel to the axis of the helix. This is the dimerization face.
Applicants avoided large, bulky amino acids along this face to minimize steric interference with dimerization WO 93/03160 ~ ~ ~ ~ PCT/US92/06412 and to facilitate formation of the stable coiled-coil structure.
Despite recent reports in the literature suggesting that methionine at positions a and d is destabilizing to 5 coiled-coils in the leucine zipper subgroup [Landschulz et al., (1989) Science 243:1681-1688 and Hu et al., (1990) Science 250:1400-1403], Applicants chose. to substitute methionine residues for leucine on the hydrophobic face of the SSP polypeptides. Methionine 10 and leucine are similar in molecular shape (Figure 3).
Applicants demonstrated that any destabilization of the coiled-coil that may be caused by methionine in the hydrophobic core appears to be compensated in sequences Where the formation of salt bridges (e-g' and g-e') 15 occurs at all possible positions in the helix (i.e., twice per heptad).
To the extent that it is compatible with the goal of creating a polypeptide enriched in lysine and methionine, Applicants minimized the unbalanced charges 20 in the polypeptide. This may help to prevent undesirable interactions between the synthetic storage proteins and other plant proteins when the polypeptides are expressed ~ Y1V0. , The polypeptides of this invention are designed to 25 spontaneously fold into a defined, conformationally stable structure, the alpha helical coiled-coil, with minimal restrictions on the primary sequence. This allows synthetic storage proteins to be custom-tailored for specific end-user requirements. Any amino acid can be incorporated at a frequency of up to one in every seven residues using the b, c, and f positions in the heptad repeat unit. Applicants note that up to 43% of an essential amino acid from the group isoleucine, leucine, lysine, methionine, threonine, and valine can be incorporated and that up to 14% of the essential WD 93/03160 PC'f/US92/06412 ~,1~.':~rjc~

amino acids from the group phenylalanine, tryptophan, and tyrosine can be incorporated into the synthetic storage proteins of this invention (see Table 2).
pol YL~~p -~~B?uenc~s The followi~~g peptides were synthesized from carboxy terminus to amino terminus on a Milligen/Biosearch peptide synthesizer using PAhTr" resin as a solid support and standard 9-fluorenylmethyloxy-carbonyl (Fmoc) chemistry for the coupling reactions.
The crude peptides were cleaved from the resin with trifluoroacetic acid and purified to homogeneity by reverse-phase high pressure liquid chromatography (HPLC). Purified peptides were used as antigens in the generation of antibodies to be used as synthetic protein detection reagents and as standards for titering antibodies. The peptides were ch~.racterized using the physico-chemical techniques described below. The synthetic storage proteins embodied in this invention are all expected to form stable dimers y~ vivo (see Tables 3 and 4).
Tabs ales of Coiled-coil ,~y,ti~ Storaae Pro 'na SSP (4),q IaEEKLKAhEEKLKAhEEKLKADEEK~KA SEQ ID N0:1 SSP ( S MEEKM1~AMEEKMFCAMEEKMICAMEEKMKA SEQ ID NO : 2 ) 4 SSP ( 7 MEEKLKAMEEKLKAMEEKLKAMEEKLKA SEQ ID NO : 3 ) 4 SSP ( 8 MEEKLKKMEEKIrKKMEEKLKKMEEKZKK SEQ ID NO : 4 ) 4 SSP ( 9 MEEKLKWMEEKZKWMEEKLKWMEEKLKW SEQ ID NO : 5 ) q SSP ( 10 MEEKMKKMEEKMKFQdEEKMICKMEEKMKK SEQ ID NO : 6 ) g SSP ( 11 MEEIi;I~CW1~EKMFEKMK~nTMEEKMKW SEQ ID NO : 7 ) 4 SSP-3-5 (A/E) MEEKhKAMEEKLKAMEEKLKAMEEKLKA
MEEKLKAMEEKI~KAM~EKHQCEMEEKi~A SEQ ID N0:112 WO 93/03160 ,~ ~'~ $ ~ PGT/US92/06412 Some of the peptides synthesi~ed include tryptophan, a limiting amino acid in animal feeds derived from maize and soybean (see Table 2). The set also includes peptides which have a net positive charge. These peptides are only a few preferred examples of the class of SSP polypeptides embodied in this invention.
Two peptides were synthesized which form alpha helical structures in aqueous solution but which were unsuitable for expression j,a vivo (Table 4).
CSP 1 MEWEELKKKLEELKKKWEEZKKKLEELKKK SEQ ID N0:8 CSP 2 MEWEEMKKKMEEMKKKWEEMKKKMEEMKKK SEQ ID Nt~ : 31 These peptides have many of the features of the synthetic storage proteins described above. However, they contain a bulky amino acid (tryptophan) on the hydrophobic surface and the salt bridges bexween positions positions a and g' or g and e' are absent.
Although circular dichroism measurements indicate that , these sequences become alpha helical in aqueous solution, a parallel coiled-coil is only one of several possible structures; they may form antiparallel two stranded coiled-coils, three stranded and four stranded parallel coils [Monera, poster, "Formation and Stability of Anti-Parallel Coiled-Coils", 9 June 1992, at Protein Engineering Meeting - 1992, Montreal, Quebec, Canada;
Alber, Presentation, "X-Ray Structure of GCN4 Leucine Zippec, a 2-Standard Parallel Coiled-Coil" 9 June 1992, at Protein Engineering Meeting - 1992, Montreal, Quebec, Canada]. This suggests that for an SSP protein to be suitable for expression in vivo, the self association v~lL~a~~

constant must be greater than the affinity of peptide monomers for cellular components.
The polypeptide chains discussed here are composed of L-amino acids and are optically active. Furthermore, the secondary structures formed by peptides in solution are also chiral. Circular Dichroism (CD) spectra of polypeptides in solution can be used to estimate the percentage of alpha helical, beta sheet, and random coil conformations in a peptide sample [Adler, (1973) Methods Enzymol. 2?:675-735]. Applicants used these techniques to characterize some example peptides which were chemically synthesized to confirm that they were conformationally stable coiled-coils in aqueous solution as designed (Example 2).
Analytical ultracentrifugation [Chervenka, C.H., (1973), A Manual of Methods for the analytical ultracentrifuge, pp. 39-64] can be used to determine the molecular weight of a protein in solution without regard to molecular shape and requiring only a knowledge of the partial specific volume of the protein. The partial specific volume can be approximated based ors amino acid composition [E. J. Cohn and J.T. Edsall, ~~Proteins, Amino Acids and Peptides", p. 370, Von Nostrandt-Reinhold, Princeton, NJ,~ 1943]. Applicants used this technique to characterize the aggregation state of SSP-3-5(A/E) (SEQ
ID N0:112), a 56 amino acid example of the synthetic storage protein family that embodies this invention. As described in Example 2 the solution was examined at three concentrations at each of two rotational speeds (28, 000 rpm and 40, 000 rpm) and two temperatures (4°C
and 20°C). No monomers were detected. Results indicate ' that this protein is a nonequilibrium mixture of stable dimers and tetramers in solution.
In vivo environments are far mare comple, than the single component aqueous solution studied above. In the WO 93/03160 ~, ~ ~ ~ ~ ~ ~ PCTlUS92/06412 presence of membranes, there are two possible mechanisms for removing the hydrophobic face of the peptide from the aqueous environment: 1) interaction with the membrane and 2) dimerization to form a coiled-coil.
Critical pressure values represent the surface pressure at which the energy required to insert a peptide into a membrane-like lipid monolayer is equal to the free energy gained in transferring the hydrophobic surface of the peptide from the aqueous solvent to the lipid. This technique is a measure of the relative affinity of various peptides for membranes as opposed to their dimerization affinities. The critical pressures of insertion by the synthetic peptides were used to compare the tendencies of the different peptides to interact with 1-palmitoyl, 2-oleyl phosphatidyl choline (POPG) monolayers and, by inference, naturally-occurring membranes composed of similar lipids.
Applicants observed no significant differences between the expression of the SSP sequences or combinations of the basic SSP sequences in ~. coli (see below, Example 6). However, the peptides, CSP 1 and CSP

2 (Table 4), cannot be expressed well in ~. coli.
Applicants speculate that as the polypeptide is , synthesized it. interacts with the bacterial cell membrane resulting in cell lysis. Although CSP series peptides appeared to form a coiled-coil structure in aqueous solutions ~g yitro, (as measured by CD) they had a high affinity for membrane surfaces as measured by the determination of changes in the surface tension of POPG
monolayers in the presence of CSP1 and/or CSP2.
Two of every four hydrophobic residues on the hydrophobic face of the CSP pegtides is tryptophan.
These residues are bulky. and may cause unfavorable steric interactions between the two polypeptide chains on dimerization. Also, in the CSP series the a - g' and ~.~_l~'l~i~
e' - g pairs are all positively charged lysine residues resulting in a repulsive electrostatic interaction between the polypeptide chains. The CSP series of polypeptides are not members of the set of synthetic storage proteins of this invention, although their properties were important in establishing the rules for defining acceptable structures for the synthetic storage proteins.
The SSP series, a preferred aspect of this invention, are distinguished from the CSP series by two important restrictions on their primary sequence. In the SSP series, only Met, Leu, Ile, Val or Thr are located in the hydrophobic core. Also, in contrast to the CSP series, the e, g, e', and g' positions in the SSP series are restricted such that an attractive electrostatic interaction always occurs at these positions between the two polypeptide chains in an SSP
dimer. These differences make the SSP series polypeptides more stable as dimers than the CSP series peptides.
The interhelical electrostatic interactions which stabilize the peptide in the dimeric state are present in the SSP structures but not in the CSP series of , polypeptides (see Figures 4b illustrating SSP4 and 4a illustrating CSP1, respectivelyf. Thus, although the CSP series fold to a stable amphipathic helix, the interchain forces are not sufficiently strong to maintain a dimeric structure in the presence of membranes or membrane-like lipid monolayers.
Interactions between the hydrophobic face of CSP series peptides and other hydrophobic molecules compete effectively with dimer formation. Studies of HIV and influenza virus derived peptide sequences have shown -that only those fusion peptides which have a critical pressure for insertion into POPG monolayers greater than 2~ ~.~~~~~

30 mN/M are likely to induce the disruption of synthetic (model) lipid bilayers ~ vitro [Rafalski et al., (1990) Biochemistry 29:7917-7922]. CSP series peptides have a critical pressure of 45 mN/M for insertion into POPG
monolayers. In contrast, SSP series peptides, which are stabilized by two interhelical salt bridges per heptad, dimerize rather than interact with other hydrophobic surfaces and have critical pressures of only 30 mN/M for insertion into POPG monolayers.
Thus, the novel synthetic storage proteins described in this invention represent a particular subset of possible coiled-coil polypeptides. Not all polypeptides which adopt an amphipathic alpha helical conformation in aqueous solution are suitable for the applications described here as was demonstrated with the CSP series of polypeptides.
The following rules derived from Applicants' work define the SSP polypeptides that Applicants claim as their invention:
The synthetic polypeptide comprises n heptad units (d a f g a b c), each heptad being either the same or different, wherein:
n is at least 4; , a and d are independently selected from the group consisting of Met, Leu, Val, Ile and Thr;
a and g are independently selected from the group consisting of the acid/base pairs Glu/Lys, Lys/Glu, Arg/Glu, Arg/Asp, Lys/Asp, Glu/Arg, Asp//Arg and Asp/Lys;
and b, c and f are independently any amino acids except Gly or Pro and at least two amino acids of b, c and f in each heptad are selected from the group consisting of Glu, ~ii~~ I J

Lys, Asp, Arg, His, Thr, Ser, Asn, Gln, Cys and Ala.
('onstruction of E. coli ExarP~sinn Vector Applicants designed the strategy shown in Figure 5 to construct the appropriate gene sequences to produce the polypeptides described above in ~. coli (see also .
Example 5). This strategy required a vector for expression of gene products in ~. ,~,oli which did not contain Earl restriction endonuclease sites. Other 14 requirements were that the vector contain the bacteriophage T7 promoter, a translation leader sequence, the transcription terminator for high level expression in ~. Vii, and appropriate cloning sites for insertion of the gene sequences described below.
Applicants also preferred the use of tetracycline resistance as a selectable marker rather than the ampicillin resistance carried on most expression vectors. Plasmids carrying the tetracycline resistance marker are less likely to be lost from cells in broth cultures because it is easier~to maintain selection pressure for plasmid maintenance.
The vector pSKS was constructed in several steps as shown in Figure 5. (1) Plasmid pSKl was a spontaneous deletion of the ampicillin gene region of pER322 including the associated Earl site. (2) Plasmid pSK2 was derived by a polymerase chain reaction (PCR) (fetus Corporation, Emeryville, CA) amplification of the entire pSKl plasmid using primers SM70 (SEQ ID N0:9) and SM71 (SEQ ID NO:10) at sites which excluded the Earl site at base 2353. (3) Plasmid pSK3 was made by removing the EcoRI site at base 1 of pSK2 by cutting with EcoRI, .
filling in the site with the Klenow fragment of DNA
palymerase and religating the vector. (4) In pBT430, a .
derivative of pET-3a [Rosenberg et al., (1987) Gene 56:125-135], the NdeI site at the ATG translation WO 93/03160 2 y "~ ~ a PCT/US92/06412 initiation site was altered by j,~ vitro mutagenesis to an NcoI site. The T7 promoter/terminator sequences from plasmid pBT430 were amplified by PCR, blunt ended by filling in With Klenow enzyme, kinased and ligated into the PvuII site of pSK3 to create pSKS. Restriction endonuclease digestion analysis and DNA sequence analysis confirmed the orientation and integrity of the PCR fragment in pSKS.
DP~~~n of CPne Sequences Based on Pe_l~tide ~aLenceR far o~ 1 ad-r_h; ~ Sy,ntt,_At i_~ Storage Prote~ n~
To produce ,,~ vivo the polypeptides described above Applicants designed DNA sequences which encoded the set of polypeptides SSP5,7,8,9,10 and 11 (SEQ ID
N0:2,3,4,5,and 6). Codons were chosen from those preferable for translation in plants and ~,. s~,~,. The DNA sequences were generated as oligonucleotides using an AHI DNA synthesizer and were inserted into a plasmid vector as shown in Figure 6.
ZO I. The first strategy for cloning these genes was as follows: The vector.pSKS was cleaved to completion with NcoI and EcoRI, mixed with kinased oligonucleotides SM80 (SEQ ID N0:14) and SM81 (SEQ ID N0:13) and ligated. , From this ligation, the plasmid pSK6 was produced which contained the "base gene" sequences of oligonucleotides SM80 (SEQ ID N0:14) and SM81 (SEQ ID N0:13). The oligonucleotide insert for this "base gene" encoded 14 amino acids (two repeats of SSP5 sequence, SEQ ID
N0:111). In addition, the "base gene" sequence had a unique Earl site into which repeated units of the various gene sequences could be inserted. Earl restriction endonuclease is unusual in that the cut site on the DNA is 4 bases 5' of the recognition site on the top strand and 1 base 3' of the recognition site on the ~~.1~'~S'~

bottom strand leaving a 5' overhang which is specific for the particular sequence:
5'-GAAGGC-3' S'-GATG~'$Q-3'~

3'-CTTCCGCTA-5' 3' -C~TCAC-5' EARI
lnucleotides 21-31 of SEQ ID N0:13 This feature allows oligonucleotides with appropriate overhanging sequences to insert in this site in only one orientation. The 21-base oligonucleotides for the representative sequences in Figure 6 were ligated to obtain multimeric sequences which were then ligated directly into the Earl site of the base gene.
Large multimers (>8n) of the 21-base oligonucleotide sets SM84/SM85 (SEQ ID N0:15/16) (SSPS) and SM82/SM83 (SEQ ID N0:17/18) (SSP7) were isolated from 18% polyacrylamide gels. The ligated oligonucleotides displayed a ladder array of multimeric forms on these gels. Bands which appeared ~o be greater than 8n (168bp) were cut from the gels, eluted and purified by ethanol precipitation. These gel-purified , oligonucleotide multimers were ligated with Earl digested pSK6 vector. Tetracycline-resistant transformants were screened by restriction-digest analysis and double-stranded sequencing. Although most inserts were 2n (42bp) or greater, no clones were isolated with greater than 5n (105bp) inserts.
Another method of enriching for multimeric forms of the insert oligonucleotides used was purification of the ligated multimeric forms by FiPLC. This method separated DNA fragments on the basis of size on a DEAE-NPR ion exchange column with a NaCl gradient. Ligated oligonucleotide sets SM82/83 (SEQ ID N0:17/18)(SSP7), _ 2114'788 SM84/85 (SEQ ID N0:15/16)(SSPS), SM86/87 (SEQ ID
N0:19/20)(SSPB), SM88/89 (SEQ ID N0:21/22)(SSP9), SM90/91 (SEQ ID N0:23/24)(SSP10), SM92/93 (SEQ ID
N0:25/26)(SSP11), were injected onto the column, eluted, 5 and fractions were collected and pooled based on known retention times for DNA size standards. Multimeric forms of the oligonucleotide sets of 6n (126bp) or greater were purified and the sizes confirmed on polyacrylamide gels. The purified oligonucleotides were 10 ligated into Earl digested pSK6 and tetracycline-resistant transformants selected in ~. ~ strain DHSa.
Clones were screened by restriction digests and sequencing. As with the gel purification, most clones had inserts of 2n (42 bp) or greater but none of the 15 inserts were greater than 6n (126 bp) in length. From this procedure Applicants obtained gene sequences coding for each of the test polypeptide sequences described previously (SEQ ID N0:2-? and 83).
Seauence by HgDtad ~ ~,~Q ID Amino Acid Re(~~SP~ 1 , ~ 7CD
NO: NO:

82-4 44 ?-?-?-7-?-7.5 45 84-H3 46 5.5.5 47 86-H23 48 5.$..,x.5 49 88-2 50 5.~-9.9.5 51 90-H8 52 5.10.10.10.5 53 92--2 54 5.1111. 5 55 lRefer to Table 3 In Table 5, the first and last SSPS heptads flanking the 20 underlined sequence represent the base gene sequence.
Insert sequences are underlined. Clone numbers including the letter "H" designate HPLC-purified oligonucleotides. The loss of the first base gene repeat in clone 82-4 (SEQ ID 44) is believed to result from homologous recombination of the base gene repeat 5.5. .
The strategy of gene construction outlined above advantageously allows the mixing of gene sequences -simply by alternating insertion of the various 21 by oligonucleotides into the Earl site. Since the Earl enzyme does not cleave at the recognition site, the site remains intact regardless of insertion at the cleavage point. Therefore, any gene sequence generated in a "first round" as above, may be lengthened and polypeptide sequences combined in any order by subsequent oligonucleotide or DNA fragment insertion into the Earl ~~'cleavage site . It may l~~e advantageous to synthesize longer oligonucleotide versions of these insert genes (ex: 84 bases = 4n) to produce longer gene sequences. Alternatively, other strategies for amplifying gene segments may be employed to construct longer gene sequences [Kempe et al., (1985) Gene.
39:239-245).
Applicants noted that even with the 2n repeat of the base gene small segments of DNA were sometimes deleted when maintained in ~. ~ strain JM103 (rec +).
This may have been due to homologous recombination. To eliminate this possibility Applicants routinely transformed the insert constructs into a ~. ~g~i strain DHSoc[supE44 del lacU169 (phi 80 lacZ del M15) hsdRl7 recAl endA1 gyr196 thil relAi)). Since these repetitive sequences may present a similar problem when transformed into recombination-proficient $arobacterium and plant cells, the DNA sequences were redesigned to be less repetitive and therefore less recombinogenic. To -do this, Applicants used alternative codons and/or mixed several polypeptide sequences. Applicants synthesized WO 93/03160 ~ ~ ~ ~ ~~ ~ ~ PCT/US92/06412 these oligonucleotides as 84-mers to assure longer initial gene sequences.
M E E K M K A M E E K M K
SM96 5'-GATGGAGGAAAAGATGAAGGCGATGGAGGAGAAAATGAAA
S SM97 . 3' CCTCCTTTTCTACTTCCGCTACCTCCTCTTTTACTTT
A M E E K M K A M E E K M K A (SEQ ID N0:2) GCTATGGAGGAAAAGATGAAAGCGATGGAGGAGAAAATGAAGGC-3' (SEQ ID N0:88) CGATACCTCCTTTTCTACTTTCGCTACCTCCTCTTTTACTTCCGCTA-5'(SEQ ID N0:89) lO M E E K L K A M E E K L K
SM98 5'-GATGGAGGAAAAGCTGAAAGCGATGGAGGAGAAACTCAAG
SM99 3' CCTCCTTTTCGACTTTCGCTACCTCCTCTTTGAGTTC
A M E E K L K A M E E K L K A (SEQ ID N0:3) GCTATGGAAGAAAAGCTTAAAGCGATGGAGGAGAAACTGAAGGC-3' (SEQ ID N0:27) 1S CGATACCTTCTTTTCGAATTTCGCATCCTCCTCTTTGACTTCCGCTA-5' (SEQ ID N0:28) M E E K L K K M E E K L K
SM100 5'-GATGGAGGAAAAGCTTAAGAAGATGGAAGAAAAGCTGAAA
SM101 3' CCTCCTTTTCGAATTCTTCTACCTTCTTTTCGACTTT
20 W M E E K L K K M E E K L K W (SEQ ID N0:83) TGGATGGAGGAGAAACTGAAAAAGATGGAGGAAAAGCTTAAATG-3'~ (SEQ ID N0:29) ACCTACCTCCTCTTTGAGTTTTTCATCCTCCTTTTCGAATTTACCTA-5' (SEQ ID N0:30) These oligonucleotides were kinased and ligated into the 25 Earl site of clones 82-4 (SEQ ID N0:44) and 84-H3 (SEQ
ID N0:46) respectively to create clones 2-9 (polypeptide sequence = SSP7.7.7.7.7.7.8.9.8.9.5) (SEQ ID N0:57), clone 3-5 polypeptide sequence ~ 7.7.7.7.7.7.5.5) SEQ TD
N0:91) and 5-1 (polypeptide sequence 30 SSP5.5.5.7.7.7.7.5) (SEQ ID N0:59). Sequences similar to these designs could be used to construct genes coding for any of the polypeptides or any combination of the polypeptides disclosed herein. The length of the coding region can be increased by a series of insertions of 35 such oligonucleotide sequences into the Earl site of the bas ~g ~e~.~ alternatively, the coding sequences can be constructed by insertion of a different base gene with any unique site for subsequent insertion of heptad coding units. Such a gene could also be constructed by generating a series of oligonucleotides with long (9-10 b) overhanging ends which could be ligated together into the NcoI/EcoRI sites of pSKS or the Earl site of pSK6 forming a several hundred base sequence.
II. A second generation of SSP genes were designed to incorporate even more variation in DNA and amino acid sequences. These sequences were designed to avoid exact repeats of sequence at the DNA level that might stimulate homologous recombination and deletion or rearrangement of the genes in bacterial or plant cells.
Again, codons were chosen which are known to be preferred in soybean, corn and E. coli [Campbell et al.
(1990) Plant Physiol. 92:1-11]. In ~.ddition, Applicants designed gene sequences to maximize stability of the mRNA based on published data on expression of foreign genes. in plant cells [Perlak, F. J., Fuchs, R. L., Dean, D. A., McPherson, S. h., and Fischhoff, D. A. PNAS 88, 3329-3328 (1991)]. All DNA sequences code for amino acid sequences that fit the parameters outlined previously.
'The strategy for construction of this second generation of gene sequences is depicted in Figures 8 and 9. The first step was the insertion of oligonucleotide sequences SM107/106 (SEQ ID:92/93) encoding a base gene of 16 amino acids into the Ncol/EcoRI sites of the vector pSKS. The features of this base gene include an unique Earl site for subsequent insertion of 63 base pair non repetitive "segments" and an unique BspIiI site at the 3' end of the gene to allow duplication of the "segments" as described in Figure 9 [Kempe et al., (1985) Gene. 39:239-245].

Crude "segment" oligonucleotide sets SM110/111 (SEQ ID
N0:94/95), SM112/113 (SEQ ID N0:98/99), SM114/115 (SEQ
ID N0: 102/103) were annealed and ligated into the Earl site to create three separate clones: pSKseg3, pSKseg9 and pSKsegS. In these "segment" clones, the DNA
sequences are as non-repetitive as possible within the limits of codon usage and amino acid sequence requirements. Applicants constructed a 107 amino acid gene sequence by joining the three gene segments using the multiplication scheme depicted in Figure 9 and described in Example 5. The resultant clone was designated pSKseg 539.
Table 6 is a summary of the relevant amino acid content of the proteins encoded by clones derived from these several cloning schemes Which were selected for transformation into plants.

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0o co o~ c~ m WO 93/03160 2 ~ ~ ~ ~ PCT/US92/06412 The SSP sequences constructed as described above were expressed in E,. cola using the bacteriophage T7 RNA
polymerase/T7 promoter system (Studier et al., (1990) Methods in Enzymology 185:60-89]. In strain BL21(DE3) [hsdS gal (lambda clts857 indi Sam7 nin5 lac W5-T7 genet)], T7 polymerase was produced~from a chromosomal copy of the polymerase coding sequence under control of the ~ promoter. Derepression of the lacZ promoter by the addition of isopropylthiogalactoside (IPTG) induced expression of T7 RNA polymerase in the cells. In strains DHSOC or HMS179 [recA hsdR rifR] there is no endogenous T7 polymerase coding sequence. The T7 polymerase gene and gene product can be delivered to these cells by infection with bacteriophage lambda CE6.
This phage carries the T7 polymerase gene and is capable of infection of these strains but is non-lytic on both strains. The procedures for inductions were as described by Studier et al., [(1986) J. Mol. Biol.
189:113-130].
Cells carrying plasmids containing the SSP coding sequences placed downstream from the T7 promoter sequence were induced with TPTG or by the addition of lambda CE6 phage during late log phase growth.
Applicants noted that the turbidity of the induced cultures tended to level off or drop slightly after induction but there was no evidence of lytic activity with induction of gene products. Samples were taken one to three hours after induction for in vivo labelling experiments using 35S methionine. Because of the high methionine content of the SSP proteins and the overexpression from the T7 promoter, Applicants expected these polypeptides would incorporate a greater amount of labelled methionine than any other protein in the cell.
35S labelling experiments were performed as described by 211~'~~8 Studier et al., [(1986) J. Mol. Biol. 189:113-130].
Unlabelled cell samples were taken one to three hours after induction and concentrated ten fold by centrifugation.
Samples of both unlabelled and labelled proteins from cell extracts were separated on 18~ (75:1.
acrylamide:bis) SDS-polyacrylamide gels. Staining of the gels with Coomassie blue revealed additional proteins produced in the induced culture samples but not in uninduced cultures. These proteins were of the approximate size expected for each of the synthetic genes described above. It should be noted that these alpha-helical proteins tended to run faster on the gel than predicted from the globular protein size standards.
In addition, there was some effect of sequence on the migration rate of particular polypeptides in the gels.
Proteins labelled with 35S methionine were visualized by autoradiography of the gels. As anticipated, the SSP
proteins Were labelled well above background levels of endogenous ~. proteins. Based on staining of ~
vitro synthesized peptides run in similar gel systems, Applicants estimate the production of these peptides to be approximately 40 mg/L of induced culture.
The size of the protein gene products and their intense labelling with 35S methionine were used to identify particular polypeptide products. In addition, Western blots of these gels Were reacted with antibodies raised against ~ vitro synthesized polypeptides SSP(5)q (SEQ ID N0:2) or SSP(7)q (SEQ ID N0:3) or with anti-GST-SSP-3-5. Secondary antibodies conjugated to horseradish peroxidase and a chemiluminescence detection system (Amersham) were used to detect the antibody-reactive proteins. Tests of the specificity of the anti-SSP antibodies using dot blots of non-denatured chemicallx synthesized peptides showed that the anti-SSP

WO 93/03160 ~ ~ ~ ~ ~ ~ Y PCT/US92/06412 antibodies cross-react with various SSP sequences.
However, when the peptides are denatured in a gel system, the reaction of the antibodies is more specific.
The antibodies raised against a particular SSP
S polypeptide reacted specifically with that SSP
polypeptide induced in ~. ~ cultures. That is, anti-SSPS reacted with proteins containing SSPS sequence and anti-SSP7 reacted with proteins containing SSP7 sequence. The specificity of these interactions confirmed the sequences of the induced proteins.
The synthetic storage proteins can also be expressed in E,. ~ as gene fusion products. The Pharmacia pGEX'i'M system was used to produce a fusion protein consisting of glutathione-S-transferase and 1S SSP-3-5. This fusion protein was purified by affinity chromatography and used to immunise rabbits. The resulting serum containing anti-GST-SSP-3-5 antibodies Was used as a detection reagent for further studies of transgenic cells expressing the SSP-3-5 protein (SEQ ID
N0:91).
This invention can be used to produce urge quantities of SSP polypeptides or total protein enriched in essential amino acids via fermentation of ~, coli or , other transformed microorganisms. The DNA sequences of the invention can be operably linked to a suitable regulatory sequence comprising a promoter sequence, a translation leader sequence and a 3' noncoding sequence.
The chimeric gene can then be introduced into a microorganism via transformation and the transformed microorganism can be grown under conditions resulting in high expression of the chimeric gene. The cells containing protein enriched in essential amino acids can be collected, the protein extracted and purified. If desired, a protein carrier such as glutathione-S-transferase can be used to simplify purification of the SSP polypeptide. If a recognition site for thrombin or factor X is included between the carrier protein and the synthetic polypeptide (as in the case of the pGEXTM
system) the SSP golypeptide can be easily separated from the carrier protein.
Alternatively, methods of purification may be based on the physical properties of the proteins or (in the case of fusion proteins) may be affinity based. The proteins are small relative to most naturally occurring proteins. They also are easily refolded from solutions of denaturant simply by removal of the denaturant. One strategy to purify the SSPs is to lyse the cells iri 6 M
urea, separate the protein mixture using gel filtration chromatography, reverse-phase chromatography or ion exchange chromatography and finally remove the urea by dialysis. Another exploitable property of the proteins is their lysine content. For those SSPs which are positively charged, anion exchange resins can be used to remove most proteins from the crude mixture without binding the SSPs.
Express o~ of SSPS in Plants A preferred class of heterologous hosts for the expression of the coding sequence of the SSP genes are a eukaryotic hosts, particularly the cells of higher plants. Particularly preferred among the higher plants and the seeds derived from them are soybean, rapeseed (~ ~,~.; ~. sam_gestris) , sunflower cotton (,~ossynium ~), corn, tobacco (~-,,~ tobacum) , alfalfa (Medicaao sativ~) , wheat (~,,sp) , barley (~vulaare) , oats (Av~na saliva, I~) , sorghum (Sor hum ~'~icolvr) , rice (Orvza sativa), and forage grasses. Expression in plants will use regulatory sequences functional in such plants.
The expression of foreign genes in plants is well-established [De Blaere et al., (1987) Meth. Enzymol.

CA 02114788 2002-~I04-17 153:277-291]. The promoter chosen to drive the expression of the coding sequence is not critical as long as it has sufficient transcriptional activity to accomplish the invention by increasing the level of 5 translatable mRNA for SSPs in the desired host tissue.
Preferred promoters for expression in all plant organs, and especially for expression in leaves include those directing the 19S and 35S transcripts in cauliflower mosaic virus [Odell et al., (1985) Nature 313:810-812;
10 Hull et al., (1987) Virology 86:482-993], small subunit of ribulose 1,5-bisphosphate carboxylase [Morelli et al., (1985) Nature 315:200; Broglie et al., (1984) Science 224:838; Hererra-Estrella et al., (1984) Nature 310:115; Coruzzi et al., (1989) EM80 J. 3:1671; Faciotti 15 et al., (1985) Bio/Technology 3:291], maize zein protein [Matzke et al., (1984) EM80 J. 3:1525], and chlorophyll a/b binding protein [Lampa et al., (1986) Nature 316:750-752] and the chemically inducible promoter In2-2 [International Publication No. W090/11361] and its derivatives 20 The selection of promoters will be driven by the specific organs of the plant where expression is desired. Preferred promoters allow expression of the protein specifically in seeds. This application is especially useful because seeds are the primary source 25 of vegetable protein. Also, seed-specific expression avoids any potential deleterious effect in non-seed organs. Examples of seed-specific promoters include, but are not limited to, the promoters of seed storage proteins, which represent more than 50% of total seed 30 protein in many plants. The seed storage proteins are strictly regulated,, being expressed almost exclusively in seeds in a highly organ-specific and stage-specific manner [Higgins et al., (1984) Ann. Rev. Plant Physiol.
35:191-221; Goldberg et al., (1989) Cell 56:199-160:
35 Thompson et al., (1989) BioEssays 10:108-113].

Moreover, different seed storage proteins may be expressed at different stages of seed development.
There are currently numerous examples for seed-specific expression of seed storage protein genes in transgenic dicotyledonous plants. These include genes from dicotyledonous plants for bean ~-phaseolin [Sengupta-Gopalan et al., (1985) Proc. Natl. Acad. Sci.
USA 82:3320-3324; Hoffman et al., (1988) Plant Mol.
Biol. 11:717-729], bean lectin [Voelker et al., (1987) EMBO J. 6: 3571-3577], soybean lectin [Okamuro et al., (1986) Proc. Natl. Acad. Sci. USA 83:8240-8244], soybean kunitz trypsin inhibitor [Perez-Grau et al., (1989) Plant Cell 1:095-1109], soybean (3-conglycinin [Beachy et al., (1985) EMBO J. 4:3047-3053: Barker et al., (1988) Proc. Natl. Acad. Sci. USA 85:458-462; Chen et al., (1988) EMBO 7. 7:297-302; Chen et al., (1989) Dev.
Genet. 10:11-122; Naito et al., (1988) Plant Mol. Biol.
11:109-123], pea vicilin [Higgins et al., (1988) Plant Mol. Biol. 11:683-695], pea convicilin [Newbigin et al., (1990) Planta 180:461], pea legumin [Shirsat et al., (1989) Mol. Gen. Genetics 215:326]; rapeseed, napin [Radke et al., (1988) Theor. Appl. Genet. 75:685-694] as well as genes from monocotyledonous plants such as for , maize 15 kD zein [Hoffman et al., (1987) EMBO J.

6:3213-3221; Schernthaner et al., (1988) EMBO J.

7:1249-1253; Williamson et al., (1988) Plant Physiol.
88:1002-1007], barley ~i-hordein [Marris et al., (1988) Plant Mol. Biol. 10:359-366] and wheat glutenin [Colot et al., (1987) EMBO J. 6:3559-3564]. Moreover, promoters of seed-specific genes operably linked to heterologous coding serrluences in chimeric gene constructs also maintain their temporal and spatial .
expression pattern in transgenic plants. Such examples include Isis 2S seed storage protein gene promoter to express enkephalin peptides in wo 93iombo ~ 11 ~ ,~ ~ ~ ~crivs9ziomz and g. seeds [Vandekerckhove et al., (1989) Bio/Technology 7:929-932], bean lectin and bean -phaseolin promoters to express luciferase [Riggs et al., (1989) Plant Sci. 63:47-57], and wheat glutenin promoters to express chloramphenicol acetyl transferase [Colot et al., (1987) EMBO J. 6:3559-3569].
Of particular use in the invention will be the heterologous promoters from several extensively-characterized soybean seed storage protein genes such as those for the Kunitz trypsin inhibitor [Jofuku et al., (1989) Plant Cell 1:1079-1093; Perez-Grau et al., (1989) Plant Cell 1:1095-1109], glycinin [Nielson et al., (1989) Plant Cell 1:313--328], ~i-conglycinin [Harada et al., (1989) Plant Cell 1:415-425]. Promoters of genes for a'- and ~-subunits of soybean ~3-conglycinin storage protein will be particularly useful in expressing the SSP mRNA in the transgenic plant cotyledons at mid- to late-stages~of soybean seed development [Beachy et al., (1985) EMBO J. 4:3047-3053; Barker et al., (1988) Proc.
Natl. Acad. Sci. USA 85:458-462; Chen et al., (1988) EMBO J. 7:297-302; Chen et al., (1989) Dev. genet.
10:112-122; Naito et al., (1988) Plant Mol. Biol.
11;.109-1.23]. This is because: a) there is very little , position effeet on their expression in transgenic seeds, and b) the two promoters show different temporal regulation: the promoter for the oc'-subunit gene is expressed a few days before that for the ~-subunit gene.
Also of particular use in the invention will be the heterologous promoters from several extensively-characterized corn seed storage protein genes such as those from the 10 kD zein [Kirihara et al., (1988) Gene 71:359-370], the 27 kD zein [Prat et al., (1987) Gene 52:51-49; Gallardo et al., (1988) Plant Sci. 54:211-281], and the 19 kD zein [Marks et al., (1985) J. Biol.
Chem. 260:16451-16459]. The relative transcriptional WO 93/03160 PC'T/US92/06412 activities of these promoters in corn are known [Kodrzyck et al., (1989) Plant Cell 1:105-114] giving a basis for choosing a promoter for use in chimeric gene constructs for corn. The promoter for the globulin 1 or globulin 2 genes of corn could also be used to express the SSP gene sequences specifically in the embryo of corn seeds [Belanger et al., (1989) Plant Physiol.
91:636-693].
Maximizing the expression of these genes in plants may also require careful attention to the design of the gene sequences to promote mRNA stability and translatability. Applicants have constructed gene sequences using preferred codons for expression in plants and bacteria. For corn and soybean, codons XUA
and XCG were not used [Campbell et al. (1990) Plant Physiol. 92:1-11]. Repeating runs of like base (AAAA,TTTT, CCCC, GGGG, etc.), poly A+ recognition sequences (AATTAA) and specific sequences that are thought to cause mRNA instability (AACCAA, ATTTA) (Perlak et al. (1991) PNAS 88:3324-3328), were avoided.
The G/C to A/T content was adjusted as close. as possible to 50/50.
The proper level of expression of SSP mRNA may , require different chimeric genes using different promoters. Such chimeric genes can be transferred into host plants either together in a single expression vector or sequentially using more than one vector.
It is envisioned that the introduction of enhancers or enhancer-like elements into the promoter constructs will also provide increased levels of primary transcription of SSP genes. These elements include viral enhancers such as that found in the 35S promoter [Odell et al., (1988) Plant Mol. Hiol. 10:263-272], enhancers from the opine genes [Fromm et al., (1989) Plant Cell 1:977-984], or enhancers from any other WO 93/03160 ~ ~ ~ ~ g g ' PCT/US92/06412 source that result in increased transcription when placed into a promoter operably linked to the nucleic acid fragment of the invention.
Of particular importance is the DNA sequence element isolated from the gene for the ot'-subunit of -conglycinin that can confer 40-fold seed-specific enhancement to a constitutive promoter [Chen et al., (1988) EI~O J. 7:297-302; Chen et al., (1989) Dev.
Genet. 10:112-122]. One skilled in the art can readily isolate this element and insert it within the promater region of any gene in order to obtain seed-specific enhanced expression with the promoter in transgenic plants. Insertion of such an~element in any seed-specific gene that is expressed at different times than the ~-conglycinin gene will result in expression in transgenic plants for a longer period during seed development.
Any 3' non-coding region capable of providing a transcription termination signal, a polyadenylation signal and other regulatory sequences that may be required for the proper expression of the S$P coding region can be used to accomplish the invention. This would include 3' end sequences from any source such thato the sequence used gives the necessary regulatory information within its nucleic acid sequence for proper expression of the promoter/SSP coding region combination to which it is operably linked. Specific examples include the native 3' end of the 10 kD zein genes) from corn, the 3' end from any storage protein gene such as the 3' end of the soybean ~-conglycinin gene or the bean phaseolin gene, the 3' end from viral genes such as the 35S or the 19S cauliflower mosaic virus transcripts, the 3' end from the opine synthesis genes, or the 3° ends of ribulose 1,5-bisphosphate carboxylase or chlorophyll a/b binding protein. The usefullness of different 3° non-WO 93/0316fl PGT/US92/06412 ~llur~g~
( 50 coding regions is taught in the art [For example, see Ingelbrecht et al., (1989) Plant Cell 1:671-680).
DNA sequences coding for intracellular localization signals may be added to the SSP coding sequence if .
required for the proper expression of the proteins to accomplish the invention. For example, the monocot signal sequence of the 10 kD xein gene could be used in corn or other monocot transformants and the signal sequence from the ~ subunit of phaseolin from the bean p~seolus ~, or the signal sequence from the a' subunit of ~-conglycinin from soybean [Doyle et al., (1986) J. Biol. Chem. 261:9228-9238], could be used in dicot transformants. Hoffman et al., [(198?) EI~O J.
6:3213-3221] showed that the signal sequence of the monocot precursor of a 15 kD zein directed the protein into the secretory pathway and was also correctly processed in transgenic tobacco seeds. However, the protein did not remain within the endoplasmic reticulum as is the case in corn. To keep the protein in the endoplasmic reticulum.it may be necessary to add stop transit sequences. It is known in the art xhat the addition of DNA sequences coding far the amino acid sequence [lys-asp-glu-leu] at the carboxyl terminal of the protein keeps proteins in the lumen of the endoplasmic reticulum [i~Iunro et al., (1987) Cell 48:899-907; Pelham (1988) Et4H0 J. 7:91.3-918; Pelham et al., (1988) EI~O J. 7:1757-1762; Inohara et al., (1989) Proc. Natl. Acad. Sci. n.S.A. 86:3564-3568; Hesse et al., (1989) EI~O J. 8:2453-2461]. In some plants seed storage proteins are located in the vacuoles of the cell. To obtain certain embodiments of the invention it may be necessary to direct the SSP proteins to the vacuole of these plants by adding a vacuolar targetting sequence. A short amino acid domain that serves as a vacuolar targetting sequence has been identified from WO 93/03160 ~ 11 ~X ~ ~ $ PCT/US92/06412 bean phytohemagglutinin which accumulates in protein storage vacuoles of cotyledons (Tague et al., (1990) Plant Cell 2:533-546]. A carboxyl-terminal amino acid sequence necessary for directing barley lectin to vacoules in transgenic tobacco has also been described [Hednarek et al., (1990) Plant Cell 2:1145-1155].
Applicants' data suggest that targetting signals are not required for accumulation of SSP proteins in tobacco seeds or rice protoplasts.
Construction of Chimeric Genes for Exflression of SSPs in Plan-A significant increase in essential amino acids in the seeds of transformed plants requires expression of high levels of SSPs in a seed-specific manner.
Expression of the synthetic storage protein sequences in plants was first accomplished by linking the SSP coding sequences to the 35S promoter from cauliflower mosaic virus and adding a 3' sequence from the nopaline synthase (NOS) gene. The strategy for this cloning is depicted in Figure 10. The plasmid pMH90 contains the -glucuronidase (GUS) gene coding sequence.,~.The SSP
sequences Were inserted into the Ncol/Asp718 sites by removing the GUS gene sequences and replacing them with the SSP NcoI/Asp718 fragments. For initial transformation experiments to investigate the efficiency of translation of the SSP sequences in plants and to observe stability and localization of SSP proteins in plant cells, trangenic tobacco plants expressing the SSP
proteins via the constitutive 35S promoter were generated.
Similar strategies were employed to insert the SSP-3-5 (SEQ ID N0:~90) or SSPseg534 (SEQ ID N0:104) gene sequences into vectors containing the phaseolin promoter and 3' sequences (CW108, ML113) or the conglycinin promoter and phaseolin 3' sequences (CW109) as in Figure 11. Fragments containing the promoter/SSP coding region/3' sequences were transferred to binary vectors as with the 35S promoter clones. These clones were utilized in subsequent transformations to generate tobacco plants.
Various methods of transforming cells of higher .
plants are available for use in Applicants' invention (see EPO 0 295 959 A2 and 0 138 341 A1). Such methods include those based on transformation vectors based on the Ti and Ri plasmids of ri,~", spp .
Particularly preferred is the binary type of these vectors [Bevan (1984) Nucl. Acids. Res. 12:8711-8720].
Ti-derived vectors transformed a wide variety of higher plants, including monocotyledonous and dicotyledonous plants, such as soybean, cotton and rape [Pacciotti et al. (1985) Bio/Technology 3:241; Byrne et al. (1987) Plant Cell, Tissue and Organ Culture 8:3; Sukhapinda et al. (1987) Plant Mol. Biol. 8:209-216; Lorz et al.
(1985) Mol. Gen. Genet. 199:178: Potrykus (1985) Mol.
Gen. Genet. 199:183].
Figure 12 shows the strategy for transferring the chimeric genes to binary vectors. Applicants cleaved the 35S::SSP::NOS chimeric gene constructs from the pSSP, plasmids by digesting the DNA with SalI. The 2.2 kb fragments were ligated individually into SalI-digested binary vector pZS97K which is part of a binary Ti plasmid vector system for ~,~"f,g-mediated plant transformation. The vector contains:
(1) the chimeric gene nopaline synthase promoter/neomycin phosphotransferase gene (nos:NPT II) as a selectable marker for transformed plant cells -[Bevan et al., (1983) Nature 304:184-186], (2) the left and right borders of the T-DNA of the Ti plasmid [Bevan, (1984) Nucl. Acids: Res. 12:8711-8720], (3) the E,. ~lj,.
lacZ a-complementing segment [Vieria et al., (1982) Gene WO 93103160 PCT/US92~/06412 211'~3~8~
19:259-267] with unique .restriction endonuclease sites for EcoRI, KpnI, BamHI and Sal I, (4) the bacterial replication origin from the p~~g, plasmid pVSl [Itoh et al., (1984) Plasmid 11:206-220], and 5) the bacterial neomycin phosphotransferase gene from Tn5 [Berg et al., (1975) Proc. Natl. Acad. Sci. U.S.A.
72:3628-3632] or ~-lactamase as selectable markers for transformation of A. tumefaciens.
The binary vectors containing the chimeric SSP
genes were transferred by tri-parental coatings [Ruvkin et al., (1981) Nature 289:85-88] to A~~robacteri~ strain LBA4904/pAL4404 [Hockema et al., (1983) Nature 303:1?9-180). The transformants were used to inoculate tobacco leaf disks [Horsch et al., (1985) Science 227:1229-1231]. Transformed plants were regenerated in selective medium containing kaisamycin or Garbenicillin.
Other transformation methods are available to those skilled in the art, such as direct uptake of foreign DNA
constructs [see EPO 0 295 959 A2], techniques of electroporation [see Fromm et al., (1986) Nature (London) 319:791) or high-velocity ballistic bombardment with metal particles coated with the nucleic acid constructs [see Kline et al., (1987) Nature (London) 327:70, and U.S. 4,945,050].
Transformed cells can be regenerated with methods understood by those skilled in the art. Of particular relevance are the recently described methods to transform foreign genes into commercially important crops, such as rapeseed [see De Block et al., (1989) Plant Physiol. 91:694-701], sunflower [Everett et al., (198?) Bio/Technology 5:1201], soybean [McCabe et al., (1988) Bio/Technology 6:923; Hinchee et al., (1988) Bio/Technoiogy 6:915; Chee et al., (1989) Plant Physiol.
91:1212-1218; Christou et al., (1989) Proc. Natl. Acad.

t' : .. .;: '''~.:r. ,:. ' ' .:.:;, . ;. ; . ... ,,.....,, ,, ,,,. ~ ,; ., z~~~~ss 54 Sci USA 86:7500-7504; EPO 0 301 749 A2], and corn [cordon-Kamm et al., (1990) Plant Cell 2:603-618; Fromm et al., (1990) Biotechnology 8:833-839]
Analysis of SSPs in t_ranseP~nt~; , Transgenic tobacco plants containing various plant promoter/SSP gene sequence constructs Were generated , using Agrobacterium mediated transformation. Applicants analyzed these plants for the presence of the genes by PCR, for the copy number of the genes by Southern blot hybridization, for the transcription of the genes by Northern blot hybridization and for the accumulation of the proteins by Western blot as described in Example 9.
A summary of this data is presented in Tables 7 and 8.
Applicants analyzed in detail transgenic plants carrying the 35S promoter/SSP3-5/NOS 3' gene sequences.
PCR generated fragments and restriction~digest analyses of the plant DNAs combined with the size of the mRNA
species visualized on the Northern blot suggest that the SSP-3-5 gene sequence (SEQ ID N0:90), despite its repetitive nature, is stable in plant cells. From the Western blotting data it is clear that the.SSP-3-5 protein (SEQ ID N0:91) is expressed in leaf tissue at levels up to 0.5% of the total cell protein (SEQ ID , N0:70). The expression level in the leaves from the 35S
promoter is positively correlated with the number of gene copies and the steady state level of mRNA.
Expression of SSP-3-5 protein (SEQ ID N0:91) in seeds from the 35S promoter is limited to about 0.01%, Since the 35S promoter is known to express poorly in seeds, this finding does not suggest instability of the protein in seeds. -Analyses of transgenic tobacco plants carrying the phaseolin promoter/SSP-3-5 coding region and the -phaseolin 3' sequences revealed that the SSP-3-5 gene sequence (SEQ ID N0:90) is stable and that expression of 211~'~88 the gene product is correlated to mRNA levels. In these plants, the level of accumulation of the protein in the seeds is estimated to be 1-2% of the total seed protein.
Amino acid analyses of the seeds from the primary 5 transformants indicate that Applicants have altered the lysine content of tobacco seeds through the introduction of genes encoding the SSP sequences. This level of SSP
expression in corn seeds would result in significant increases in lysine acid and methionine content.
The present invention is further defined in the following Examples, in which all parts and percentages are by weight and degrees are Celsius, unless otherwise stated. It should be understood that these Examples, while indicating preferred embodiments of the invention, are given by way of illustration only. From the above discussion and.these Examples, one skilled in the art can ascertain the essential characteristics of this invention, and without departing from the spirit and scope thereof, can make various changes and modifications of the invention to adapt it.to various usages and conditions. All publications including patents cited by Applicants herein are incorporated in .
their entirety by reference.
2 5 Eli C_h_e_m__i_ca1_ Svnthe!~i a of Coiled-coil P~lt,jdes Peptides described in Fables 3 and 4 were synthesized on a Milligen 9050 solid phase peptide synthesizer using standard protocols suggested by the manufacturer. For peptides containing an alanine at position 21 the peptide was double-coupled at that position.
The columns were packed with 0.1 meq 9-fluorenyl-methyloxycarbonyl (Fmoc) PAL'A'M Resin (Milligen/Biosearch) mixed with four mass equivalents of glass beads (Sigma).

WO 93/03160 ~ PCT/US92/06412 211~'~8$ 56 The following protected Fmoc derivatives of the amino acids (Milligen/Biosearch) were used: Fmoc-L-Ala-O-pentafluorophenyl ester, Fmoc-L-Lys(N-tert-butoxy-carbonyl)-O-pentafluorophenyl ester, Fmoc-L-Met- .
pentafluorophenyl ester, Fmoc-L-Glu(O-t-butyl)-O-pentafluorophenyl ester, Fmoc-L-Trp-0-pentafluorophenyl , ester. In some cases nonesterified amino acids (Advanced ChemTech) mixed with 300 mg 2-(1H-benzo-triazol-1-yl)-1,1,3,3-tetramethyluronium hexafluoro-phosphate (HBTU) were employed.
An analog of the 56 amino acid peptide, SSP-3-5 (one amino acid change: Ala to Glu) SSP-3-5 (A/E) MEEKLKAMEEKLKAMEEKLKAMEEKLKAMEEKLKA
MEEKLKAMEEKMKEMEEKLKA (SEQ ID N0:112) was synthesized on a Milligen/Biosearch Excell peptide synthesizer. The reaction vessel contained 0.05 meg F-moc PAh Resin (Milligen/Biosearch). The same protected Fmoc amino acids were used as above. The synthesis program was modified to acetylate unreacted peptide chains after each coupling reaction with a solution of acetic anhydride, pyridine, and 4-dimethylaminopyridine~
in dimethylformamide. The first alanine was double coupled. All coupling times were increased two to threefold over the manufacturer°s suggestion.
Following completion of each synthesis, the resin was removed from the column by rinsing with methanol into a coarse sintered glass funnel. The material was - 30 washed well with methanol and then with diethyl ether.
For cleavage of the peptide from the resin, it was placed into a 20 mL roundbottom flask. Five mL of cleavage solution [9.5 mL trifluoroacetic acid, 0.25 mL
thioanisole, 0.15 mh ethanediol, 0.1 mL anisole] were added. The flask was stoppered and shaken on a rotary shaker at 200 rpm for 2 h at room temperature. The resin was then filtered through a coarse sintered glass frit into a 125 mL erlenmeyer flask. The resin was washed with 3 to 5 mL of trifluoroacetic acid and the 5 washings combined with the filtrate. A stream of nitrogen was used to reduce the volume of the filtrate to 5 mL. Ice cold diethyl ether was added to precipitate the peptide (approximately 20 to 30 mL).
The peptide was filtered through a fine sintered glass 10 frit and washed with cold diethyl ether. The material was then dissolved with distilled water and lyophilized subsequent to further purification by reverse-phase HPLC.
Separations Were performed on VYDAC C9 or Clg 15 semipreparative (1 cm diameter) or preparative (1 inch diameter) columns at a flow rate of approximately 4 mL/min/cm2 using a two buffer system [buffer A: 0.1%
trifluoroacetic acid (Pierce) in water (MilIiQTM); buffer B: 90% acetonitrile (JT Baker), 10% water, 0.1 %
20 trifluoroacetic acid]. Gradients varied with peptide sequence but typically began at close to 30% buffer B
and linearly increased buffer B at a rate of 0.25%/min.
The effluent was monitored at 220 nm and the major peak collected. The collected material was lyophilized and 25 stored dry at 4°. Purified material was analyzed by fast atom bombardment mass spectroscopy, amino acid composition and, in some cases, protein sequencing to confirm the identity and purity of the synthetic materials.

~yrg; ra 1 ('hs~rat~_tnr~ ~s~t; nn of Synthetic Pert ides Surface pressure was determined by the de Noy method [Adamson, (1976) The Physical Chemistry of Surfaces, Third Ed. John Wiley & Sons, NY] using a 35 Fisher Autotensiomat Model 215. For monolayer insertion experiments, a monolayer of 1-palmitoyl, 2-oleyl phosphatidylcholine (POPG) was spread from a chloroform solution (concentration approximatly 0.1 mg/ml) onto the surface of 10.5 mI. of HEPES/MES/citrate-buffered saline -[5 mM HEPES, 5 mM MES, 20 mM sodium citrate, 25 mM
sodium chloride, pH 7.4] contained in a polytetra-fluoroethylene beaker. The surface area of the resultant POPG monolayer was approximately 5 cm2.
Surface tension was measured and adjusted to various desired values by withdrawing a glass capillary tube through the surface to remove a portion of the previously spread monolayer. When the desired value had been attained, 0.1 mL of a 1 mg/mI. solution of the peptide dissolved in the above-mentioned buffer was injected beneath the surface with an hypodermic syringe, care being taken to minimi2e removal of the monoiayer, and the surface tension monitored until no change was evident over approximatly 10 min observation time.
This procedure was repeated for a number of different initial lipid monolayer surface tensions, including determinations in the absence o~.lipid and with no peptide injection to determine the surface tension of the buffer alone. Data calculation involved, subtraction of the latter value from all other measured values to provide defined "surface pressure" values.
The change in surface pressure caused by injection of peptide was plotted against the initial surface pressure and critical insertion pressure determined by extrapolation to zero surface pressure change. These critical pressure values were used to compare the tendencies of the different peptides to interact with POPG monolayers and, by inference, naturally occuring membranes composed of similar lipids. The CSP peptides -were found to have a eritical pressure of 45 ~ 3 mN/M

2~~.~788 whereas the SSP peptides were found to have a much lower critical pressure of 30 ~ 3 mN/M.
The synthetic peptide SSP-3-5(A/E) (SEQ ID N0:112) was characterized by analytical ultracentrifugation using a Beckman Model E ultratracentrifuge and refractive index detection. The peptide was dissolved in a phosphate buffer [50 mn sodium phosphate, pH 7.0, 150 mM NaCl) at three concentrations: 0.4 mg/mL, 1.2 mg/mL, and 3.5 mg/mL. For each of these concentrations, runs were made at two rotational speeds (28,000 rpm and 40,000 rpm) and at two temperatures (4°C and 20°C). The results of all the experiments are best fit by two noninterconverting species in solution: a dimer and a tetramer. No monomers were detected. These findings support Applicants' assertion that these sequences may adopt stable dimeric structures (coiled-coils) in aqueous solution and in vivo under physiological conditions.
ExB~E~rF~..3 Purified ~ vitro-synthesized peptides described in (Example 1) were dissolved in distilled water to 1 mg/mL. To denature the peptides l.O mL of,xhis solution was mixed with 10 ~1L of 10% sodium dodecyl sulfate (SDS, BRL, Gaithersburg, MD) and heated at 65° for 10 min.
One mL of peptide,solution (either the denatured or the native preparation) was mixed with 100 p1L of '75 ug/mL
keyhole limpet hemocyanin (Sigma), 30 ~iL of freshly opened 25% glutaraldehyde (Sigma) and 1.0 mL of phosphate buffered saline [PBS: 8.0 g NaCl/L, 0.2 g KCl/L, 1.44 g Na2HP04/L, 0.24 g KHZP04/L, pH 7.2]. The mixture was allowed to rock on a table top rocker for 3 h at room temperature. The mixture was sonicated for a few seconds to break up flocculent and was dialyzed (molecular weight cutoff: 10,000) overnight at 9° with two changes of 1 L of PBS. The preparation was sent to Hazelton laboratories in Denver, PA fox infection into ~ ~ ~ ~ ri rabbits. Rabbit sera were collected on a biweekly basis. Sera containing antibodies produced after multiple injections with antigen were used to.test specificity against ~ vitro and ~ vivo (Example 6) 5 synthesized peptides.
Antibodies derived from this procedure were tested for specificity by reacting with ~ vitro-synthesized peptides which had been transferred to nitrocellulose membrane using a BRL dot blotting apparatus. The 10 peptides Were diluted in tris buffered saline [TBS:
25 mM TrisCl, pH 8.0, 8.0 g NaCl/L, 0.2 g KC1/L] to give solutions of 10 ~,g/~tL, 1 ~,g/E1h or 0.1 ~ig/~iL. Two hundred microliters of each dilution were applied to the membrane using the dot blot apparatus following 15 manufacturer's specifications. A dilution series of non-denatured (native) SSP(5)q (SEQ ID N0:2), SSP(7)q (SEQ ID N0:3), SSP(8)q (SEQ ID N0:4), SSP(9)q (SEQ ID
N0:5) , SSP (10) q (SEQ ID N0:6), or SSP (11) q (SEQ ID N0:'7) peptides was loaded onto the nitrocellulose membrane in 20 replicates, the membrane was then sectioned into strips and reacted with antibodies raised against denatured or native SSP(5)q or SSP(7)q peptides at a serum dilution of 1:500. Secondary goat anti-rabbit antibodies conjugated to alkaline phophatase (Bio-Rad, Richmond, 25 CA) were used with 5-bromo-4-chloro-3-indolyl phosphate p-toluidine salt and vitro blue.tetrazolium chloride to visualize the primary rabbit anti-peptide antibody reactions as described by the manufacturer. From these interactions it was determined that the antibodies 30 prepared against native (coiled-coil) structures of peptides SSP(5)q (SEQ ID N0:2) or SSP('~)q (SEQ ID N0:3) .
cross-reacted With all the other native peptides and detected as little as 1 ug. The antibodies reacted with .
their own counterpart peptides to detect down to 0.01 ~1g 35 of peptide. Cross reactivity to all other peptides was WO 93/03160 1 ~~ '~ $ ~ PCT/US92/06412 less when interacting With the antibodies raised against the denatured peptides SSP(5)q (SEQ ID N0:2) and SSP(7)q (SEQ ID N0:3).
One ~tg samples of peptides SSP(5,7,8,9,10 or 11)q were denatured in loading buffer (4% SDS, 20% glycerol, 0.1 M Tris pH 8.0, 9% ~-mercaptoethanol, 0.01%
bromphenol blue), boiled for 2 min and loaded onto a 12 X 10 X 0.075 cm 18% polyacrylamide SDS denaturing gel (acrylamide: bis-acrylamide = 50:0.66) [Laemmli, U.K.
(1970) Nature 227:680]. The samples were electrophoresed through the gel at 150 volts for 2.5 h.
Western blots Were performed as follows. The peptides were then transferred from the gels to 0.2 micron nitrocellulose membrane (Biorad) in a buffer which contained 2.4 g Tris base, 11.25 g glycine, 1 g SDS, 200 mL methanol/liter buffer using a Hoofer transblot apparatus at 30 volts for 30 min followed by 80 volts for 30 min. The blots were air dried. Duplicate blots were then reacted with anti-denatured SSP(5)q antibody or anti-denatured SSP(7)q (SEQ ID N0:3) antibody at a serum dilution of 1:500. Visualization with secondary antibody conjugated to alkaline phosphatase, followed by 5-bromo-4-chloro-3-indolyl phosphate p-toluidine salt and vitro blue tetrazolium chloride showed detection of 1 ~.g of SSP(5)4 peptide with anti SSP(5)~ antibody and no cross reaction with 1 dig of SSP (7, 8, 9,10, or 11) q peptides. One ~.g of SSP(5)q and SSP(7)q peptides could be visualized with anti-SSP (?)q antibody with no cross reaction with 1 ~,g of SSP (8, 9,10,11) q peptides .
A fusion protein of glutathione-S-transferase and the SSP3-5 gene product was generated through the use of the PharmaciaTM pGEX GST Gene Fusion System (Current Protocols in Molecular Biology, Vol 2, pp 16.7.1-8, (1989) John htiley and Sons). The fusion protein was purified by affinity chromatography or.. glutathione yV0 93/03160 PCT/US92/06412 ~~1~~ ~~~ 62 agarose (Sigma) or glutathione sepharose (Pharmacia) beads, concentrated using Centricon 10TH' (Amicon) filters, and then subjected to SDS polyacrylamide electrophoresis (15% Acrylamide, 19:1 Acrylamide:Bis-acrylamide) for further purification. The gel was stained with Coomassie Blue fox 30 min, destained in 50%

Methanol, 10% Acetic Acid and the protein bands electroeluted using an Amicon'~ Centiluter Microelectroeluter (Paul T. Matsudaira ed., A Practical Guide to Protein and Peptide Purification for Microsequencing, Academic Preys, Inc. New York, 1989).

A second gel prepared and run in the same manner was stained in a non acetic acid containing stain [9 parts 0.1% Coomassie Blue 6250 (Bio-Rad) in 50% methanol and 1 part Serva Blue (Sesva, Westbury, NY) in distilled water] for 1-2 h. The gel was briefly destained in 20%

methanol, 3% glycerol for 0.5 - 1 h until the GST-SSP3-5 band Was just barely visible. This band was excised from the gel and sent with the electroeluted material to Hazelton Laboratories for use as an antigen in immunizing a New Zealand Rabbit. A total,pf 1 mg of antigen was used (0.8 mg in gel, 0.2 mg in solution).

Test bleeds were provided by Hazelton Laboratories ever, three weeks.. The approximate titer was tested by western blotting of E. Coli extracts from cells containing the SSP-3-5 gene under the control of the T7 .

promoter (Example 6) at different dilutions of protein and of serum. Known amounts of chemically synthesized protein corresponding to the SSP-3-5 (SEQ ID N0:91) sequence with only one amino acid change (SEQ ID N0:112) (see Example 1) were loaded on gels to test the limits of detection with the antibody in Western blotting protocols. Using the test bleed obtained 18 weeks after initial immunization Applicants could detect 2 ng of chemically synthesized peptide at a 1:4000 dilution of WO 93/03160 ~ ~ 1 ~ 7 g g PCT/US92/06412 antibody and overnight incubation of the blot with the diluted serum. This serum was used to measure levels of SSP-3-5 (SEQ ID N0:91) expression in transgenic plants (Example 9) and to confirm the identity of radiolabelled SSP-3-5 protein (SEQ ID N0:91) in rice protoplasts (Example 10 ) .
ES~AI~LE 4 ~onstrLC ion of E oli ~;nn Vecrnr To facilitate the construction and expression of the synthetic genes described below in Example 5, it was necessary to construct a plasmid vector with the following attributes:
1. No Earl resrriction endonuclease sites such that insertion of sequences in Example 5 would produce a unique site.
2. Encoding tetracycline resistance to avoid . loss of plasmid during growth and expression of toxic proteins.
3. Containing approximately 290 by from plasmid pBT430 including the T7 promoter and terminator segment for expression of inserted sequences in ~.
4. Containing unique EcoRI and NcoI
restriction endonuclease recognition sites in proper location behind the T7 promoter to allow insertion of the oligonucleotide sequences in Example 5.
To obtain attributes 1 and 2 Applicants used plasmid pSKl which was a spontaneous mutant of pBR322 where the ampicillin gene and the Earl site near that gene had been deleted (see Figure 5). Plasmid pSK1 retained the tetracycline resistance gene, the unique EcoRI restriction sites at base 1 and a single Earl site at base 2353. To remove the Earl site at base 2353 of pSKl a polymerise chain reaction (PCR) was performed using pSKl as the template. Approximately 10 femtomoles of pSKl were mixed with 1 ~tg each of oligonucleotides WO 93/03160 . PCf/LJS92/05412 SM70 and SM71 which had been synthesized on an ABI1306B
DNA synthesizer using the manufacturer's procedures.
SM70 5'-CTGACTCGCTGCGCTCGGTC 3' SEQ ID N0:9 SM71 5'-TATTTTCTCCTTACGCATCTGTGC-3' SEQ ID NO:10 The priming sites of these oligonucleotides on the pSKl template are depicted in Figure 5. The PCR was performed using a Perkin-Elmer Cetus kit (Emeryville, CA) according to the instructions of the vendor on a thermocycler manufactured by the same company. The 25 cycles were 1 min at 95°, 2 min at 92° and 12 min at 72°. The oligonucleotides were designed to prime replication of the entire pSKl plasmid excluding a 30 b fragment around the Earl site (see Figure 5). Ten .microliters of the 100 ~1L reaction product were run on a 1% agarose gel and stained with ethidium bromide to reveal a band of about 3.0 kb corresponding to the predicted size of the replicated plasmid.
The remainder of the PCR reaction mix ( 90 ~tL) was mixed with 20 E1L of 2.5 mM deoxynucleotide triphosphates EdATP, dTTP, dGTP, and dCTP), 30 units of lClenow enzyme added and the mixture incubated at 37° for 30 min followed by 65° for 10 min. The Klenow enzyme was used°
to fill in ragged ends generated by the PCR. The DNA
was ethanol precipitated, washed with 70$ ethanol, dried under vacuum and resusperided in water. The DNA was then treated with 'r4 DNA kinase in the presence of 1 mM ATP
in kinase buffer. This mixture was incubated for 30 mans at 37° followed by 10 min at 65°. To 10 ~tL of the kinased preparation, 2 ~1L of 5X ligation buffer and 10 units of T4 DNA ligase were added. The ligation was carried out at 15° for 16 h. Following ligation, the DNA was divided in half and one half digested with Earl enzyme. The Klenow, kinase, ligation and restriction WO 93/03160 ~ 1 ~ !~ ~ $ $ PCT/US92/06412 endonuclease reactions were performed as described in Sambrook et al., [Molecular Cloning, A Laboratory Manual, 2nd ed. (1989) Cold Spring Harbor Laboratory Press]. Klenow, kinase, ligase and most restriction 5 endonucleases were purchased from BRL. Some restriction endonucleases were purchased from NEN Biolabs (Beverly, MA) or Boehringer Mannheim (Indianapolis, IN). Both the ligated DNA samples were transformed separately into competent JM103 [supE thi del (lac-proAB) F' [traD36 10 porAB, lacIq lac2 del M15] restriction minus] cells using the CaCl2 method as described in Sambrook et al., (Molecular Cloning, A Laboratory Manual, 2nd ed. (1989) Cold Spring Harbor Laboratory Press] and plated onto media containing 12.5 ug/mL tetracycline. With or 15 without Earl digestion the same number of transformants were recovered suggesting that the Earl site had been removed from these constructs. Clones were screened by preparing DNA by the alkaline lysis miniprep procedure as described in Sambrook et al., [Molecular Cloning, A
20 Laboratory Manual, 2nd ed. (1989) Cold Spring Harbor Laboratory Press] followed by restriction endonuclease digest analysis. A single clone was chosen which was tetracycline-resistant and did not contain any Earl sites. This vector was designated pSK2. The remaining.
25 EcoRI site of pSK2 was destroyed by digesting the plasmid with EcoRI to completion, filling in the ends with Klenow and ligating. A clone which did not contain an EcoRI site Was designated pSK3.
To abtain attributes 3 and 4 above, the 30 bacteriophage T7 RNA polymerase promoter/terminator segment from plasmid pBT430 was amplified by PCR.
Plasmid pBT430 is a derivative of pET-3a [Rosenberg et al., (1987) Gene 56:125-135]. The T7 promoter/
terminator sequence was cloned into the BamFII site of 35 -pBR322 to make plasmid pET-3a. Plasmid pBT430 was WO 93/03166 ~ ~ j~''~

constructed by converting the NdeI site at the ATG
translation initiation of plasmid pET-3a to an NcoI site using oligonucleotide directed mutagenesis. The DNA
sequence of pET-3a in this region, 5'CATATGG, was changed to 5'-CCCATGG in pBT430. Oligonucleotide primers SM78 (SEQ ID N0:11) and SM79 (SEQ ID N0:12) were designed to prime a 300b fragment from pBT930 spanning the T7 promoter/terminator sequences (see Figure 5).
SM78 5'-TTCATCGATAGGCGACCACACCCGTCC-3' SEQ ID NO:11 SM79 S'-AATATCGATGCCACGATGCGTCCGGCG-3' SEQ ID NO:12 The PCR reaction was carried out as described previously using pBT430 as the template and a 300 by .fragment was generated. The ends of the fragment were filled in using Klenow enzyme and kinased as described above. DNA from plasmid pSK3 was digested to completion with PwII enzyme and then treated with calf intestinal alkaline phophatase (Boehringer Mannheim) to remove the 5' phosphate. The procedure was as described in Sambrook et al., [Molecular Cloning, A Laboratory Manual, 2nd ed. (1989) Cold Spring Harbor Laboratory Press]. The cut and phosphatased pSK3 DNA was purified~
by ethanol precipitation and a portion used in a ligation reaction with the PCR generated fragment containing the T7 promoter sequence. The ligation mix was transformed into JM103 (supE thi del (lac-proAB) F' [traD36 porAB, lacIq lacZ del M15] restriction minus]
and tetracycline-resistant colonies were screened.
Plasmid DNA was prepared via the alkaline lysis mini prep method and restriction endonuclease analysis was performed to detect insertion and orientation of the PCR
product. Two clones were chosen for sequence analysis:
Plasmid pSKS had the fragment in the orientation shown y.:7y..., w .., ~ . t:eS.W ~.. . .. . . .. . ' n . ...
WO 93/03160 ~ (~'~ ~ $ PCT/US92/06412 s~
in Figure 5. Sequence analysis performed on alkaline denatured double-stranded DNA using Sequenase~ T7 DNA
polymerase (US Biochemical Corp) and manufacturer's suggested protocol revealed that pSKS had no PCR
replication errors within the T7 promoter/terminator sequence.
I.
(a) The strategy for the construction of repeated synthetic gene sequences based on the Earl site is depicted in Figure 6. The first step was the insertion of an oligonucleotide sequence encoding a base gene of 14 amino acids. This oligonucleotide insert contained a .unique Earl restriction site for subsequent insertion of oligonucleotides encoding one or more heptad repeats and added an unique Asp7lS restriction site for use in transfer of gene sequences to plant vectors. The overhanging ends of the oligonucleotide set allowed insertion into the unique NcoI and EcoRI sites of vector s pSKS.
M E E K M K A M E E K
SM81 ~ 5'-~,~AGGAGAAGATGAAGGCGATG~BG$~aAAG
SM80 3'-~TCCTCTTCTACTTCCGCTAC~TTC
NCOI EARI
M K A (SEQ ID NO:111) 3 Q SM81 ATGAAGGCGTGATAQQ~$~~3' (SEQ ID N0:13) SM$0 TACTTCCGCACTAT;S~""~5' (SEQ ID N0:14) DNA from plasmid pSKS was digested to completion with NcoI and EcoRI restriction endonucleases WO 93/031b0 PGT/LJS92/06412 and purified by agarose gel electrophoresis. Purified DNA (0.1 ug) was mixed with 1 dig of each oligonucleotide SM80 (SEQ ID N0:14) and SM81 (SEQ ID N0:13) and ligated.
The ligation mixture was transformed into ~. ~ strain JM103 [supE thi del (lac-proAB) F' [traD36 porAB, lacIq lacZ del M15] restriction minus] and tetracycline resistant transformants screened by rapid plasmid DNA
preps followed by restriction digest analysis. A clone Was chosen which had one each of Earl, NcoI, Asp718 and EcoRI sites indicating proper insertion of the oligonucleotides. This clone was designated pSK6 (Figure 7). Sequencing of the region of DNA following the T7 promoter confirmed insertion of oligonucleotides of the expected sequence.
(b) Repetitive heptad coding sequences were added 'to the base gene construct of (a) above by generating oligonucleotide pairs Which could be directly ligated into the unique Earl site of the base gene.
Oligonucleotides SM84 (SEQ ID N0:15) and SM85 (SEQ ID
N0:16) code for repeats of the SSPS heptad.
Oligonucleotides SM82 . (SEQ ID N0:17) and S~~I83 (SEQ ID
N0:18) code for repeats of the SSP7 heptad.
SSPS . M E E K M K A (SEQ ID N0:62) 2 5 SM84 5'-GATGGAGGAGAAGATGAAGGC-3 (SEQ ID N0:15) SM85 3'- CCTCCTCTTCTACTTCCGCTA-5' (SEQ ID N0:16) SSP7 M E E K I. K A 6SEQ ID N0:61) SM82 5-GATGGAGGAGAAGCTGAAGGC-3 (SEQ ID N0:17) 3 0 SM83 3"- CCTCCTCTTCGACTTCCGCTA-5' (SEQ ID N0:18) s Oligonucleotide sets were ligated and purified to obtain DNA fragments encoding multiple heptad repeats for insertion into the expression veetor. Oligonucleo-35 tides from each set totalling about 2 ~tg were kinased, yVU 93/03160 ~~ ~ 6 ~''j ~ $ PCT/US92/06412 and ligated for 2 h at .room temperature. The ligated multimers of the oligonucleotide sets were separated on an 18% non-denaturing 20 X 20 X 0.015 cm polyacrylamide gel (Acrylamide: bis-acrylamide = 19:1). Multimeric forms which separated on the gel as 168 by (8n) or larger were purified by cutting a small piece of polyacrylamide containing the band into fine pieces, adding 1.0 mL of 0.5 M ammonium acetate, 1 mM EDTA
(pH 7.5) and rotating the tube at 37° overnight. The polyacrylamide was spun down by centrifugation, 1 ~.g of tRNA Was added to the supernatant, the DNA fragments were precipitated with 2 volumes of ethanol at -70°, washed with 70% ethanol, dried, and resuspended in 10 ~1L of Water.
Ten micrograms of pSK6 DNA were digested to ,completion with Earl enzyme and treated with calf intestinal alkaline phosphatase. The cut and phosphatased vector DNA Was isolated following electrophoresis in a low melting point agarose gel by cutting out the banded DNA, liquifying the agarose at 55°, and purifying over MACS PREPAC'~'"~ columns (BRL) following manufacturer's suggested procedures.
Approximately 0.1 [tg of purified Earl digested and phosphatase treated pSK6 DNA was mixed with 5 [tL of the~
gel purified multimeric oligonucleotide sets and ligated. The ligated mixture was transformed into ~.
~, strain JNl103 [supE thi del (lac-proAB) F' [traD36 porAB, laclq lacZ de1 M15] restriction minus] and tetracycline-resistant colonies selected. Clones were screened by restriction digests of rapid plasmid prep DNA to determine the length of the inserted DNA.
Restriction endonuclease analyses were usual~..y carried out by digesting the plasmid DNAs with Asp718 and BglII, followed by separation of fragments on I8% non-denaturing polyacrylamide gels. Visualization of WO 93/03160 PCT/US92/Oi412 fragments with ethidium bromide, showed that a 150 by fragment was generated When only the base gene segment was present. Inserts of the oligonucleotide fragments increased this size by multiples of 21 bases. From this 5 screening several clones were chosen for DNA sequence analysis and expression of coded sequences in ~. coli (see Example 6) .
Se~egce by Hertad Clone SEO ID NO: ~ no Acid Re~jS~~ E EO ID NO:
~k C15 32 5.7.7.7-7.7.5 33 C20 34 5.7.7_7.7,7.5 35 C30 36 5.7.7.7.7.5 37 D16 38 5.,5.x,.5 39 ~D20 40 5.5.5.5.5 41 D33 42 5 .:~.. 5 43 lRefer to Table 3 The first and last SSPS heptads flanking the sequence of each construct are from the base gene of seetion (a) 10 above. Inserts are designated by underlining.
(c) Because the gel purification of the oligomerac forms of the oligonucleotides did not give the expected enrichment of longer (i.e., >8n) inserts, Applicants used a different procedure for a subsequent round of 15 insertion constructions. For this series of constructs four more sets of oligonucleotides were generated which code for SSP 8,9,10 and 11 amino acid sequences respectively:

WO 93/03160 ~ ~ ,) i ,-' ~ ~ P~.T/US92/a6412 SSPB M E E K I. K K (SEQ IDN0:69) SM86 S'-GATGGAGGAGAAGCTGAAGAA-3' (SEQ IDN0:19) SM87 3'- CCTCCTCTTCGACTTCTTCTA-5' (SEQ IDN0:20) SSP9 M E E K L K W (SEQ IDN0:67) SM88 5-GATGGAGGAGAAGCTGAAGTG-3' (SEQ IDN0:21) SM89 3'- CCTCCTCTTCGACTTCACCTA-5' (SEQ IDN0:22) SSP10 M E E K M K K (SEQ IDN0:6S) lO SM90 5'-GATGGAGGAGAAGATGAAGAA-3' (SEQ IDN0:23) SM91 3'- CCTCCTCTTCTACTTCTTCTA-5' (SEQ IDN0:24) SSP11 M E E K M K W (SEQ IDN0:68) SM92 5'-GATGGAGGAGAAGATGAAGTG-3' (SEQ IDN0:25) SM93 3'- CCTCCTCTTCTACTTCACCTA-5' (SEQ IDN0:26) The following HPLC procedure Was used to purify multimeric forms of the oligonucleotide sets after kinasing and ligating the oligonucleotides as described in section (b) above. Chromatography was performed on a Hewlett Packard Liquid Chromatograph instrument, Model 1090Nl. Effluent absorba~ce was monitored at 260 nm: higated oligonucleotides were centrifuged at 12,OOOxg for 5 min and infected onto a 2.5 ~. TSK DEAE-NPIt ion exchange column X35 cm x 4.6 mm I.D.) fitted with a 0.5 ~.in-line filter (Supelco). The oligonueleotides were separated on the basis of length using a gradient elution and a two buffer mobile phase [Buffer A: 25 mM Tris-C1, pH 9.0, and Buffer B: Buffer A + 1 M NaCl~. Both Buffers A and B were passed through 0.2 ~! filters before use. The following gradient program was used with a flow rate of 1 mL per min at 30°:

WO 93/03160 PCTlUS92/06412 ~~..1.'.srl~c~

initial 75 25 0.5 min 55 45 min 50 50 5 20 min 38 62 23 min 0 100 30 min 0 100 31 min 75 25 Fractions (500 ~1L) were collected between 3 min and 9 min. Fractions corresponding to lengths between 120 by and 2000 by were pooled as determined from control separations of restriction digests of plasmid DNAs.
The 4.5 mL of pooled fractions for each oligonucleotide set were precipitated by adding 10 ~tg of .tRNA and 9.0 mL of ethanol, rinsed twice with ?0~
ethanol and resuspended in 50 ~1L of water. Ten yaicroliters of the resuspended HDLG purified oligonucleotides were added to 0.1 ~tg of the Earx cut, phosphatased pSK6 DNA described above and ligated overnight at 15°. All six oligonucleotide sets described above which had been kinased anc~ self-ligated but not purified by gel or HPLC were also used in separate ligation reactions with the pSK6 vector. The ligation mixtures were transformed into ~. strain DHSac [supE49 del lacU169 (phi 80 lacZ del M15) hsdRl7 recAl endAl gyr196 thil relAl] and tetracycline-resistant colonies selected. Applicants chose to use the DH5~c [supE44 del lacU169 (phi 80 lack del M15) hsdRl7 recAl endA1 gyr196 thil relAl] strain for all subsequent work because this strain has a very high transformation rate acrd is r~cA-. The recA- phenotype eliminates concerns that these repetitive DNA structures ~ may be substrates for homologous recombination leading to deletion of multimeric sequences.

' ' ~s~ :' r~; ~ - a rf~:~i'.'. " . . '~:v .,, ~'. ;' ~.,; . .......: ~ . :~ : :, .,': :~
WO 93/03160 _ ~ ~ ~ ~ ~. ~ ~ PCT/US92/06412 Clones were screened as described in (b) above. Several clones were chosen to represent insertions of each of the six oligonucleotide sets.
S~auence by~H tad Clone # SEO ID NO: Am3 no A _; d R ,~_eat (SSp) 1 ~~O 2D NO:
82-9 49 1.7.7.7_7.7.5 45 84-H3 9 6 5 .5~.. 5 4 7 86-H23 48 5..$x.5 4g 88-2 50 5.9.9.9.5 51 90-H8 52 5 .10.10.10.5 53 92-2 54 5.1111.5 55 lRefer to Table 3 r The first and last SSPS heptads flanking the sequence represent the base gene sequence. Insert sequences axe underlined. Clone numbers including the letter "H"
designate HPZC-purified oligonucleotides. The lass of the first base gene repeat in clone 82-4 may have resulted from homologous recombination bet~teen the base gene repeats 5.5 before the vector pSK6 Was transferred to the recA- strain. The FiPLC procedure did not enhane~
insertion of longer multimeric forms of the oligonucleotide sets into the base gene but did serve as an efficient purification of the ligated oligonucleotides.
(d) Oligonucleotides were designed which coded for mixtures of the SSP sequences and which varied codon ' usage as much as possible. This was done to reduce the possibility of deletion of repetitive inserts by recombination once the synthetic genes were transformed into plants and to extend the length of the constructed gene segments. These oligonucleotides encode four .. :..,..,..,, ,, . ;;::::v.:. ; ..... ..:
WO 93/03160 ~ ..~ ~ ~~~ PCTlUS92/06412 repeats of heptad coding units (28 amino acid residues) and can be inserted at the unique Earl site in any of the previously constructed clones. SM96 and SM97 code for SSP(5)4 (SEQ ID N0:2), SM98 and SM99 code for SSP(7)q (SEQ ID N0:3) and SM100 plus SM101 code for SSP8.9.8.9 (SEQ ID N0:83).
M E E K M K A M E E K M K
SM96 5'-GATGGAGGAAAAGATGAAGGCGATGGAGGAGAAAATGAAA
SM97 3' CCTCCTTTTCTACTTCCGCTACCTCCTCTTTTACTTT
A M E E K M K A M E E K M K A (SEQ ID N0:2) GCTATGGAGGAAAAGATGAAAGCGATGGAGGAGAAAATGAAGGC-3' (SEQ ID N0:88) CGATACCTCCTTTTCTACTTTCGCTACCTCCTCTTTTACTTCCGCTA-5' (SEQ ID N0:89) SM98 5'-GATGGAGGAAAAGCTGAAAGCGATGGAGGAGAAACTCAAG
SM99 3' CCTCCTTTTCGACTTTCGCTACCTCCTCTTTGAGTTC
A M E E K L K A M E E K L K A (SEQ ID N0:3) GCT'ATGG~I~IGAAAAGCTTAAAGCGATGGAGGAGAAACTGAAGGC-3' (SEQ ID N0:27) ZO CGATACCTTCTTTTCGAATTTCGCATGCTCCTCTTTGACTTCCGCTA-5' (SEQ ID N0:28) M E E K L K K M E'E K L K
SM100 5'-GATGGAGGAAAAGCTTAAGAAGATGGAAGAAAAGCTGAAA
SM101 3' CCTCCTTTTCGAATTCTTCTACCTTCTTTTCGACTTT
25 W M E E~ K L K K M E E K L K W (SEQ ID N0:83) TGGATGGAGGAGAAACTCA~,AAAGATGGAGGAAAAGCTTAAATG-3' (SEQ ID N0:29) ACCTACCTCCTCTTTGAGTTTTTCATCCTCCTTTTCGAATTTACCTA-5' (SEQ ID N0:30) DNA from clones 82-A and 89-H3 (see c above) 34 were digested to completion with Earl enzyme, treated with phosphatase and gel purified. About 0.2 ~.g of this DNA were mixed with 1.0 dig of each of the oligonucleotide sets SM96 and SM97, SM98 and SM99 or SM100 and SM101 which had been previously kinased. The 35 DNA and oligonucleotides were ligated overnight and then WO 93/03160 ~ ~ ~ ~ ~ y PCT/US92/06412 the ligation mixes transformed into E.. coli strain DHSa.
Tetracycline-resistant colonies were screened as described in sections (b) and (c) for the presence of the oligonucleotide inserts. Clones were chosen fox sequence analysis based on their restriction endonuclease digestion patterns.
_S~ ~ -n -~ htr Henrari Clone ~ SEO ID NO~ Amino Acid R_D a (SS )1 SEO ID NO:
2-9 S6 7.7.7.7.7.7.8.9.8.A.5 57 3-S 90 7.7.7.7.7.7.5.5 91 S-1 58 5.5.5.?.7.7.7.5 5g lRefer to Table 3 Inserted oligonucleotide segments are underlined Clone 2-9 was derived from oligonucleotides SM100 (SEQ ID N0:29) and SM101 (SEQ ID N0:30) ligated into the Earl site of clone 82-4 (see section (e) above). Clone 3-S (SEQ ID N0:90) was derived from the insertion of the first 22 bases of the oligonucleotide set SM96 (SEQ ID N0:88) and SM97 (SEQ ID N0:89) into the Earl site of elope 82-4 (SEQ ID N0:44). This partial insertion may reflect improper annealing of these highly repetitive oligos. Clone 5-1 (SEQ ID N0:58) was derived from oligonucleotides SM98 (SEQ ID N0:27) and SM99 (SEQ
ID N0:28) ligated into the Earl site of clone 84-H3 (SEQ
ID N0:46) (see section (c) above) .
II.
(a) A second strategy for construction of synthetic gene sequences was implemented to allow more flexibility in both DNA and amino acid sequence. This strategy is depicted in Figures 8 and 9. The first step was the insertion of an oligonucleotide sequence WO 93/03150 ~ . PCT/US92/06412 iL~.~ i ~~ ~6 encoding a base gene of 16 amino acids into the original vector pSKS. This oligonucleotide insert contained an unique Earl site as in the previous base gene construct for use in subsequent insertion of oligonucleotides encoding one or more heptad repeats. The base gene also included a BspHI site at the 3' terminus. The overhanging ends of this cleavage site are designed to allow "in frame" protein fusions using NcoI overhanging ends. Therefore, gene segments can be multiplied using the duplication scheme described in Figure 9. The overhanging ends of the oligonucleotide set allowed insertion into the unique NcoI and EcoRI sites of vector pSKS.
M E E K M K K I. E E K
SM107 5'-CATGGAGGAGAAGATG~I~AAAAGCTCGAAGAGAAG
SM106 3'-CTCCTCTTCTACTTTTTCGAGCTTCTCTTC
NCOI EAgI
M K V M K (SEQ ID N0:113) ATGAAGGTCATGAAGTGATAGGTACCG-3' (SEQ ID N0:92) TACTTCCAGTACTTCACTATCCATGGCTTAA-5' (SEQ ID N0:93) The oligonucleotide set was inserted into pSKS vector as described in I (a) above. The resultant plasmid was designated pSK34.
(b) Oligonucleotide sets encoding 35 amino acid "segments" were ligated into the unique Earl site of the pSK34 base gene using procedures as described in I~b) In this case, the oligonucleotides were not gel or HPhC
purified but simply annealed and used in the ligation reactions. The following oligonueleotide sets were used:

WO 93/03160 ~, ~ ~ ~ PCT/US92/06412 SM110 5'-GCTGGAAGAAAAGATGAAGGCTATGGAGGACAAGATGAAATGG
SM111 3'-CCTTCTTTTCTACTTCCGATACCTCCTGTTCTACTTTACC
L E E K M K K (SEQ ID N0:85) (amino acids 8-28) CTTGAGGAAAAGATGAAGAA-3' (SEQ ID N0:94) GAACTCCTTTTCTACTTCTTCGA-5' (SEQ ID N0:95) SM112 5'-GCTCGAAGAAAGATGAAGGCAATGGAAGACAAAATGAAGTGG
SM113 3'-GCTTCTTTCTACTTCCGTTACCTTCTGTTTTACTTCACC
L E E K M K K (SEQ ID N0:97) (amino acids a-28) CTTGAGGAGAAAATGAAGAA-3' (SEQ ID N0:98) ' GAACTCCTCTTTTACTTCTTCGA-5' (SEQ ID N0:99) SM114 5'-GCTCAAGGAGGAAATGGCTAAGATGAAAGACGAAATCTGGAAA
SM115 3'-GTTCCTCCTTTACCGATTCTACTTTCTGCTTTACACCTTT
L K E E M K K (SEQ ID NO:101) (amino acids 8-28) 2 5 ~ CTGAAAGAGGAAATGAAGAA (SEQ ID N0:102~
GACTTTCTCCTTTACTTCTTCGA (SEQ ID N0:103) Clones were screened for the presence of the inserted segments by restriction digestion followed by separation of fragments on 6% acrylamide gels. Correct insertion of oligonucleotides was confirmed by DNA sequence analyses. Clones containing segments 3,4 and 5 respectively were designated pSKseg3, pSKseg4, and pSKsegS.
These "segment" clones were used in a duplication scheme as described in Figure 9.. Ten ug of Pcr/us9a/o6ali WO 93/03160 ~ ~ ~ ~~ ~~ ~ ~ 7 8 plasmid pSKseg3 were digested to completion with NheI
and HspHI and the 1503 by fragment isolated from an agarose gel using the Whatmann paper technique (see Example 7). Ten ug of plasmid pSKseg4 were digested to completion with NheI and NcoI and the 2109 by band gel isolated. Equal amounts of these fragments were ligated and recombinants selected on tetracycline. Clones were screened by restriction digestions and their sequences confirmed. The resultant plasmid was designated pSKseg34.
pSKseg34 and pSKsegS plasmid DNAs were digested, fragments isolated and ligated in a similar manner as above to create a plasmid containing DNA
sequences encoding segment 5 fused to segments 3 and 4.
This construct was designated pSKseg534 and encodes the .following amino acid sequence:
SSP534 NH2-MEEFQKKKLKEEMAICMf~EMWKLKEEMKKLEEXMKVMEEKMKKLEEIQdKA
MF:DIQ4ICwLEEFQ~ICRLEEKMKVI~lEEKMKKLEEKMKAMEDKMKWT.EEIQ~LCIC
2 0 LEEICMKVMIC-COOH ( SEQ ID DTO : Z 0 S ) f~AMP LE 6 s ~ression of Proteins from Synthetic Cene ~,g~P,~ces in E . coli To detect expression of the polygeptides encoded by the synthetic genes described in Example 5, the plasmid DNAs were maintained in DHSac [supE44 del lacU169 (phi 80 lacZ del M15) hsdRl7 recA1 endA1 gyr196 thil relA1]
cells or transformed into ~. coli strain HMS174 [recA
hsdR rifR] or HL21(DE3) [hsdS gal (lambda clts857 indi Sam7 nin5 lac UV5-T7 genet]. Tn DHSoc [supE44 del lacU169 (phi 80 lacZ del M15) hsdRl7 recAl endAl gyr196 thil relAl] or HMS179 [recA hsdR rifR] cells, the expression of proteins by addition of T7 polymerase was achieved by infection of the late log phase cells with WO 93/03160 ~ l~ ~ g $ PCT/US92/06412 lambda phage CE6 at a multiplicity of infection of 0.3.
In BL2i(DE3) (hsdS gal (lambda cIts857 indi Sam7 nin5 lac WS-T7 genel) cells, the T7 polymerase gene product was expressed by derepression of the lacZ promoter with addition of isopropylthiogalactoside (IPTG) (HRL). Both procedures were performed as described by Studier et al., [(19861 J. Mol. Hiol. 189:113-130].
One to three h after induction, samples of the induced cells were collected and concentrated 10-fold by centrifugation and resuspended in lysis buffer [100 mM
NaCl, 50 mM, Tris pH 8.0, 1 mM EDTA) . Samples (10 ~1L) of the concentrated cells were lysed by boiling in an equal volume of SDS dye buffer for several min then loaded onto an 18% polyacrylamide SDS denaturing gel as described in Example 3. Alternatively, 100 ~iL samples were removed from induced cultures, washed twice with M9 .minimal media [Sambrook et al , Molecular Cloning, A
Laboratory Manual, 2nd ed. (1989) Cold Spring Harbor Laboratory Press], resuspended in M9 minimal medium and labelled with 35S methionine. The labelling procedure was performed as described by Studier et al., ((1986) J.
Mol Hiol. 189:113-130). Since these polypeptides have a high percentage of methionine, it was expected that they would label better than any other proteins in the ~.
~ cells. After 10 min the labelled cells were concentrated 10-fold by centrifugation. The concentrated cells were lysed as with the unlabelled samples and loaded onto SDS-PAGE gels.
The gels were fixed and stained in 50%
trichloroacetic acid, 10% acetic acid, 0.1% Coomassie Blue for 30 min, then destained in 50% methanol, 10%
acetic acid for 30 min followed by overnight destaining in 5% methanol, 7% acetic acid. The fixed and destained gels were vacuum-dried and the gels containing labelled WO 93/03160 ~ ~ ~ ~'"l $ ~ ~ PC'T/US92/06412 _ ao proteins were placed in film cassettes with X-ray film for overnight exposure.
With Coomassie staining, the polypeptide gene products were visible for most of the smaller (<8n) gene constructs. There were no ~. proteins with molecular weight of less than 7,000 daltons visible on the gels. This facilitated visualization of these <6,000 dalton polypeptide gene products. They represented up to an estimated 40 mg/Z of original induced cell culture. Induced polypeptides were also seen as intensely labelled bands on the autoradiographs of the 35S methionine labelled samples. The SSP
polypeptides tend to tun somewhat faster than predicted by globular size standards in this gel system. The size of each of the overexpressed polypeptide bands was proportional to the specific gene sequences determined I in Example 5.
a WO 93/03160 ~ ~ ~~ 4 v ~ ~ PCT/US92/06412 Pol~e~t ide Clone ~ S EO ~D NO: Site in Kil_odalto~ SEO ID NO:
(by Calculation) D16 38 3.464 39 82-4 44 5.954 45 84-H3 4 6 3 . 9 64 47 86-H23 48 3.542 49 88-2 50 4.622 S1 90-H8 52 4.502 53 92-2 54 3.695 55 2-9 56 9.691 57 3-5 90 6.820 91 5-1 58 6.856 59 ~seg 3 84 4.466 85 seg .4 96 4.466 97 seg 5 100 4.466 101 *seg 34 86 8.932 87 *sea 534 104 13.398 105 *~,'hese proteins have not been tested for expression in H.
The identity of some of the overproduced polypeptides was confirmed by Western blotting and reaction with anti-SSP antibodies as described in Example 3. More sensitive eietection was made possible by the use of secondary antibodies conjugated to horseradish peroxidase~and chemiluminescent visualization. This procedure was performed according to the manufacturer's directions (Amersham), Although the anti-SSP(5)q and anti-SSP(7)q antibodies interact with other ~. ~ proteins (-7000-8,000 daltons), they could be used to detect the smaller molecular weight-induced polypeptide products. Anti-SSP(5)q reacted with WO 93/03160 ~ ~ ~ ~ ~ ~ 82 PCT/~JS92/06412 polypeptides from genes containing mostly SSPS repeats such as 84-3 (SEQ ID N0:47) and 5-1 (SEQ ID N0:59) but not polypeptides from genes containing only SSP7 repeated sequences such as 82-4 (SEQ ID N0:45) and 3-5 (SEQ ID N0:91). Anti-SSP(7)q reacted with polypeptide from clones 82-4 (SEQ ID N0:95) and 3-5 (SEQ ID N0:91) (mostly SSP7 repeats) but less well with polypeptide from Clone 5-1 (SEQ ID N0:59) where the SSP7 repeats are contained within SSPS repeats.
The anti GST SSP-3-5 antibody described in Example 3 was used to detect the SSP-3-5 protein (SEQ ID N0:91) in bacterial induction samples. Applicants detected protein in induction samples diluted to 1:100 (approximately 20 ng of protein). The specificity of this antibody was tested against induction samples of .all other synthetic proteins (see Table 5). It had limited cross reactivity With proteins from clones 86-H23 (SEQ ID N0:49) and 88-2 (SEQ ID N0:51), more reactivity with proteins from clones D16 (SEQ ID N0:39) and 84-H3 (SEQ ID N0:47) and strong reactivity with protein from clone 82-4 (SEQ ID N0:45) (precursor to clone 3-5 (SEQ ID N0:91)).
FXAMPLE 7 , Constriction of Chimeric Genes for ~~,pression of Synthetic Gene Prod~~Lcts in Plants To express the synthetic gene products described in Example 6 in plant leaf cells, the sequences were transferred from the E,. coli expression vector pSK6 or pSK34 to plas~iid pl~gi40 (see Figure 10) . Plasmid pl~gi40 contains the 35S 5' promoter sequences from cauliflower mosaic virus, the 5' translation leader sequence from the chlorophyll a/b binding protein gene, the coding region for the b-glucuronidase (GUS) gene, and the NOS
3' non-coding sequences. This plasmid has been designed with a unique NcoI restriction site at the ATG

- 8 ~ ~ ~ ~ ~ ~ PCT/US92/05412 translation initiation codon and an unique Asp718 site just 3' to the translation stop codon.
To replace the GUS gene coding sequence with the synthetic storage protein gene sequences, 10~1g of pMFi40 DNA were digested to completion with Asp718 and NcoI
restriction endonucleases. The digestion products were separated on a 1.0% agarose gel by overnight electrophoresis at 15 volts. The 5,080 by vector fragment containing the 35S promoter and NOS 3' sequences was separated from the smaller fragment containing the GUS coding region. The 5,080 by fragment was collected by cutting the agarose in front of the band, inserting a 10 X 5 mm piece of Whatman 31~i paper into the agarose and electrophoresing the fragment into the paper [Errington, (1990) Nucleic Acids Research, 18:17]. The fragment and buffer were spun out of the paper by centrifugation and the DNA in the -100 ~.L was precipitated by adding 10 mg of tRNA, 10 ~ti, of 3 M
sodium acetate and 200 ~tL of ethanol. The precipitated DNA was washed twice with 70% ethanol and dried under vacuum. The fragment DNA was resuspended in 20 ~iL of water and a portion diluted 10-fold for use in ligation reactions.
Plasmid DNA (10 mg) from each of the clones 82-4, 84-H3, 86-H23, 88-2, and 90-H8, 3-5 and pSK534 was digested to completion with Asp718 and NcoI restriction endonucleases. The digestion products were separated on an 18% polyacrylamide non-denaturing gel as described in Example 5. Gel slices containing the desired fragments were cut fro the gel and purified by inserting the gel slices into a 1% agarose gel and electrophoresing for 20 min at 100 volts. DNA fragments were collected on 10 X
5 mm pieces of Whatman 3I~ paper, the buffer and fragments spun out by centrifugation and the DNA
precipitated with ethanol. The fragments were ~1L'~~~,8 84 resuspended in 6 ~lL water. One microliter of the diluted pMH40 fragment described above, 2 ~lL of SX
ligation buffer and 1 ~iL of T4 DNA ligase were added.
The mixture was ligated overnight at 15°~
The ligation mixes were transformed into ~. coli strain DHSa [supE44 del lacU169 (phi 80 lacZ del M15) hsdRl7 recAl endAl gyr196 thil relAl] and ampicillin-resistant colonies selected. The clones were screened by restriction endonuclease digestion analyses of rapid plasmid DNAs and by DNA sequencing. DNA (from clones containing synthetic gene sequences inserted between the 35S promoter and the NOS 3' sequences were digested with SalI endonuclease to completion. The 35S
promoter:: synthetic gene::NOS.3' sequences were contained on a 2100 b SalI fragment. This fragment was isolated from a 1% agarose gel on Whatman 3MM paper as Example 7.
To express the SSP's to high levels in seeds, the same coding regions were transferred by similar procedures as described above to the seed promoter vectors CW108, CW109 or ML113 (Figure I1). The vectors CW108 and ML1I3 contain the bean phaseolin promoter(from base +1 to base -494), and 1191 bases of the 3' sequences from bean phaseolin gene. CW109 contains the soybean ~-conglycinin promoter (from base +1 to base -619) and the same 1191~bases of 3' sequences from the bean phaseolin gene. These vectors were designed to allow direct cloning of coding sequences into unique Ncol and Asp718 sites. These vectors also provide sites (HindIII
or SalI) at the 5' and 3' ends to allow transfer of the promoter/coding region/ 3' sequences directly to appropriate binary vectors.

~;1~.~~'~88 E?CAMPLE 8 Transformation of Tobacco with the SSP Chimers~ ,PnP~
The binary vectors pZS97 or pZS97K were used to transfer the chimeric genes to plants. Both binary 5 vectors pZS97 and pZS97K (Figure 12) are part of a binary Ti plasmid vector system [Bevan, (1984) Nucl.
Acids. Res. 12:8711-8720] of Ag,robacterium ~m faciens.
The vectors contain: (1) the chimeric gene nopaline synthase::neomycin phosphotransferase (nos::NPTII) as a 10 selectable marker for transformed plant cells [Bevan et al., (1983) Nature 304:184-186], (2) the left and right borders of the T-DNA of the Ti plasmid [Bevan, (1984) Nucl. Acids. Res. 12:8711-8720], (3) the E,. coli lacZ
a-complementing segment [Viering et al., (1982) Gene 15 19:259-267] with a unique SalI site(pSK97K) or unique ,HindIII site (pZS97) in the polylinker region, (4) the bacterial replication origin from the Pseudomonas plasmid pVSl [Itoh et al., (1984) Plasmid 11:206-220], and (5) the bacterial neomycin phosphotransferase gene 20 from Tn5 [Berg et al., (1975) Proc. Natl. Acad. Sci.
U.S.A. 72:3628-3632] (pZS97K) or the bacterial -lactamase gene (pZS9?) as selectable markers for transformed $. tumefaciens.
Plasmid pZS97K DNA was digested to completion with 25 SalI enzyme and the digested plasmid was gel purified as described in Example 7. The Sall digested pZS97K DNA
was mixed with the isolated chimeric gene fragments from Example 7 and ligated at 15° overnight. The ligation mixes were transformed into DHSa [supE44 del lacU169 30 (phi $0 laeZ del M15) hsdRl7 recAl endA1 gyr196 thil relAl] cells and kanamycin-resistant colonies were selected. Plasmid DNAs from kanamycin-resistant colonies were screened by restriction digest analyses and DNA sequencing for the presence of the chimeric 35 genes.

WO 93/03160 ~ ~ ~ p "'l ~ ti 8 6 PCT/1JB92/06412 Plasmid pZS97 DNA was digested to completion with HindIII enzyme and the digested plasmid was gel purified as decribed in Example 7. The HindIII digested pZS97 DNA was mixed with the isolated chimeric gene fragments from Example 7, ligated, transformed as above and colonies selected on ampicillin.
Binary vectors containing the chimeric genes were transferred by tri-parental coatings [Ruvkin et al., (1981) Nature 289:85-88] to 8grobact,~~ium strain LBA4404/pAL4904 [Hockema et al., (1983), Nature 303:179-180] selecting for kanamycin or carbenieillin resistance. Cultures of Agrobacterium containing the binary vector were used to transform tobacco leaf disks [Borsch et al., (1985) Science 227:1229-1231].
Transgenic plants were regenerated in selective medium ,'containing kanamycin and screened for the presence of the transgenes as described in Example 9.

ANALYSIB OF TRANSGENIC TOBACCO PLANTS
I. EXTRACTION OF NUCLEIC ACIDS FROM LEAVES
Leaves from plants containing the 35S promoter and the gene for SSPs from 'clones 86-H23 (SEQ hD N0:48) , 88-2 (SEQ ID NO:SO), 90-H8 (SEQ ID N0:52) and 3-S (SEQ ID
N0:90) were used to prepare DNA and RNA extracts. These extracts were~used for PCR, Southern and Northern blot analysis. Two grams of leaf tissue, derived from leaves 10 cm in length were placed into a mortar containing liguid nitrogen on dry ice. The tissue was ground to a fine powder. This powder was added to 10 mL of extraction buffer [50mM Tris, pH 9.0, 10 mM EDTA, 2%
SDS] at 50°C in a sterile 50 mL polyethylene centrifuge tube. Five milligrams of proteinase K were added and ' the mixture incubated at SO°C for 10 min with occasional mixing. The solution was extracted twice with ' phenol: chloroform:isoamyl alcohol [25:24:1] and twice WO 93/03160 ~ ~ ~ ~ ~ ~ PCT/US92/06412 '87 with chloroform:isoamyl alcohol [24:1]. The aqueous layer was brought to 0.3M sodium acetate and precipitated with 2.5 volumes of ethanol. The pellet, containing the nucleic acids was washed with 70% ethanol and vacuum dried. The pellets were resuspended in 10.0 mL of water, mixed with an equal volume of 4M LiCl2 and allowed to precipitate on ice for one h. The precipitated RNA was collected by centrifugation at 10,000 rpm for 25 min at 4°C, resuspended in 5.0 mL of water and reprecipitated twice more with LiCl2 as above.
After the third precipitation, the solution was brought to 0.3M sodium acetate and precipitated with 2.5 volumes of ethanol, washed with 70% ethanol, centrifuged, and resuspended for use in northern blot analysis. The original supernatant containing the DNA was precipitated .with 1 volume of isopropanol on dry ice, collected by .centrifugation, washed with 70% ethanol and resuspended for use in PCR and southern blot analyses.
II. EXTRACTION OF RNA FROM SEEDS
To analyze expression of SSP gene sequences in seed tissue, RNA was extracted by grinding 200 mg of seeds s collected 19 days after flowering under liquid nitrogen to a fine powder with mortar and pestle. The powder was then extracted, twice by adding 3 mL phenol:
chloroform:isoamyl alcohol X25:29:1) and 4.5 mL of extraction buffer [1M Tris pH 9.0,1% SDS, 5% ~-mercapto-ethanol]. Nucleic acids in the supernatant were precipitated by adding 1/lOth volume 3M sodium acetate and 2.5 volumes of ethanol. The precipitate was collected by centrifugation, washed with SO% ethanol and air dried. The pellet was then resuspended in 4 mL
water and 1 mL 10 M LiCl2 was added. RNA was precipitated at 4° for 1 hour, collected by centrifugation and the pellet resuspended in 500 ~lL H20.

WO 93/03164 ~ ~ ~ ~ ~ r~ ~ ~ 8 8 ,..;,,, III. PCR ANALYSIS OF PLANT DNAS
To confirm the presence of the SSP gene sequences in the transgenic tobacco plants, extracted DNA was used as the template in polymerase chain reactions. .
Oligonucleotide primers which annealed to specific promoter and 3' sequences of the transgenes were used. .
The oligonucleotide primers were as follows:
For 35S promoter sequences:
3551 5'-TTTGGAGGAGGACACG-3' (SEQ ID N0:106) For NOS 3' sequences:
MEH15 5'-AAGAGAGAATTGAGAC-3 (SEQ ID N0:107) For phaseolin promater sequences:
1S SM124 5'-GTACTACTACTCTACTACT-3' (SEQ ID N0:108) For phaseolin 3' sequences:
SM125 5'-GAGCTCTTACACCTACATGCA-3' (SEQ ID N0:109) For ~-conglycinin promoter sequences:
SM126 5'-CATCAAGAACCAGTTCAATA-3' (SEQ ID NO:110) PCR reaction mixtures included 0.1-0.3 Elg of each primer, 1 ~Lg of plant DNA, 4 ~iL of 2.5mM dNTPS, 5 ~iL of lOX reaction buffer (800mM Tris pH 9.0, 200mM (NH4) 2S04, lSmM MgCl2), 1 mL Perfect MatchTM (Stratagene, La Jolla, 2S CA) and H20 to a f final volume of 4 9 ~tL . After an initial denaturation at 9S° for 5 mires, 1 ~xL of TAQ''~°
polymerase was added to each reaction and the following.
cycle program was run: 1 min at 95°, 1 min at 42° and 3 min at 72° for 40 cycles. PCR generated DNA fragments were separated on 1.2 % agarose gels and visualized with ethidium bromide. Bands generated were 100 to 200 bases longer than the SSP coding regions depending on the .
construct. Plants with correct PCR fragments were designated as positive for the transgene (see Tables 7 and 8) .

IV. SOUTHERN BLOT ANALYSES OF PLANT DNAS
DNAs derived from PCR positive plants were further analyzed by digesting 10 ~tg with BamHI or Asp718 to 5 completion, separating the fragments on a 1.0% agarose gel, transferring the DNA to HybondT" M membrane using 20X
SSC [Maniatis) and hybridizing the blot with a digoxigenin labelled DNA fragment appropriate to the transgene. Blotting procedures, digoxigenin labelling of probe fragment, hybridization and wash conditions and antibody visualization of signal were as described for the GeniusT"" blotting kit (USB). Southern blotting analyses for the 35S:SSP3-S:Nos 3' transgenic plants are summarized in Table 7.
V. NORTHERN BLOT ANALYSES OF PLANT RNAS:
RNAs isolated as described above were separated on 1% agarose gel containing 3% formaldehyde in 5 mM sodium tetraborate, 0.18 mM disodium ethylenediaminetetracetic 20 acid. Separated RNAs were transferred to ZetaprobeT"' membrane using 20X SSC, the blot hybridized with an appropriate 32P labelled DNA probe fragment (probe fragments included promoter, SSP coding region and 3' DNA sequences) at 45°, washed three times with 2X SSC, 25 0.1% SDS at 25°, then 3X with O.1XSSC, 0.1% SDS at 55°
and the blot sutoradiographed. Relative levels of SSP
RNA message are summarized in Tables 7 and 8.
VI. EXPRESSION OF SSP PROTEINS IN TRANSGENIC TOBACCO

Leaves from plants containing the 35S promoter and the gene for SSP-3-5 were also used to prepare protein extracts. The protein extracts were prepared from leaves 10 cm in length. The central vein was removed 35 and and tissue cut into pieces and weighed. Extraction WO 93!03160 PCT/US92/06412 23~~~18~ 90 buffer (1 mL/g tissue): C50 mM Tris~Cl, 1 mM EDTA, 50 mM
NaCl, pH 7.5~ was added to the tissue in a small mortar on ice. The tissue Was ground for approximately 10 min until a smooth paste was obtained. An additional 1 mL
of extraction buffer Was added for each gram of tissue and the grinding was continued for 5 min longer. The slurry was transferred to eppendorf centrifuge tubes and spun at 10,000 rpm for 15 min at 4°C. The supernatant was removed to a new tube, frozen and respun at full speed in the eppendorf centrifuge for 5 min. The concentration of protein in the sample was determined using the Biorad protein assay and bovine serum albumin as a standard. The supernatant was diluted, mixed with 27t SDS dye buffer (Enprotech) , and boiled for 2 man prior to loading 1 ~tg onto 17 to 27% gradient SDS gels.
.The gels were blotted onto 0.2 micron nitrocellulose (Biorad), and developed by reaction with the anti-GST-SSP-3-5 followed by chemiluminescent detection (Amersham) as discussed in Example 5. Results of the Western analyses of leaf tissue are presented in Table 7.
From the Western blotting data it is Glear that the SSP-3-5 protein (SEQ ID N0:9I) is expressed in leaf tissue at levels up to 0.5% of the total cell protein. , The:~expression. level in the leaves from the 35S promoter is positively correlated with the number of gene copies and the steady state level of mRNA. Expression of SSP-3-5 protein (SEQ ID N0:91) in seeds from the 35S promoter is limited to about 0.01%. Since the 35S promoter is known to express poorly in seeds, this finding does not suggest instability of the protein it seeds.
VII. EXP:tESSION OF SSPs IN TOBACCO SEEDS
Dried seeds, harvested from transgenic tobacco plants were ground at room temperature in a mortar and "..
WO 93/03160 ~ ~. ~ l~ ~ ~ ~ PCT/US92/06412 pestle with a total of four volumes of extraction buffer (12.5 mM sodium borate, pH 10.0, 1% SDS, 2% 2-mercapto-ethanol, 1 mM phenylmethylsulfonylfluoride]. Grinding was initiated with two volumes of buffer. After a paste was achieved, additional phenylmethylsulfonylfluoride was added to bring the final concentration to 2mM and the final two volumes of buffer were added. The resultant slurry was frozen and then centrifuged in an eppendorf centrifuge for 15 min at 9°C. The supernatant was removed to another tube and the concentration determined using the Biorad Laboratories (Hercules, CA) protein assay with bovine serum albumin as a standard.
Samples were diluted, SDS gel loading buffer added and electrophoresis was performed on daiichi intermediate gels (10-20% acrylamide). Transfer to nitrocellulose ,was performed as in Example 5 and the synthetic storage protein detected using polyclonal rabbit anti-GST-SSP3-5 serum. Results of the western analyses of these plants are presented in Table 8.
Amino acid analysis was performed on seeds of these plants. Fifteen mature seeds were placed in.a pyrolyzed glass test tube with 100 ~1L 6N HC1, (double distilled, 5 mL V4COR ampules), 0.4% mercaptoethanol. The suspension was degassed with argon and the tube sealed , under argon. .The samples were incubated at 110°C-125°C
for 20-24 hours. The tubes were cracked open, the contents dried and resuspended in 500 ~iL of dilution buffer (Beckman) containing 2 nmoles of norleucine. per 50 ~iL. Fifty mL were injected onto a Beckman System 6300 amino acid analyzer equipped with an ion exchange amino acid analysis column. The analysis was run according to the manufacturer's recommendations.
Eighteen amino acids axe analyzed. Lysine is expressed as a percent of total amino acids analyzed. The results are shown in Table 8.

WO 93/031b0 PCT/US92/06412 Analyses of transgenic tobacco plants carrying the phaseolin promoter/SSP-3-S coding region and the , phaseolin 3' sequences revealed that the SSP-3-5 gene sequence (SEQ ID N0:90) is stable and that expression of the gene product is correlated to mRNA level. In these plants, the level of accumulation of the protein in the seeds is estimated to be 1-2% of the total seed protein.
This level of SSP expression in corn seeds would result in significant increases in lysine and methionine content.

21~~'~88 a~ a a~
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EXPRESSION OF SYNTHETIC STORAGE
PROTEINS TN RICE PROTOg,LASTS
CONSTRUCTION OF THE PhASMID 508/SK29 5 The SSP-3-5 gene was combined with a chemically inducible monocot promoter HPH508 [International Publication No. W090/11361] for use in transient expression assays with rice protopiasts (see Figure 13). The chemically inducible promoter was 10 chosen to allow Applicants to maximize expression through addition of the chemical inducer.
The construction of the plasmid pd24 has been described in International Publication No. W090/11361.
15 Plasmid p029 was digested to completion with the restriction endonucleases SnaHI and HpaI. The digestion products were separated on a 10%
polyacrylamide gel. The 76 by SnaBI/HpaI fragment was excised from the gel and eluted from the polyacrylamide 20 by shaking overnight at 37°C in elution buffer (Maniatis reference]. Another aliquot of plasmid p~29 was digested to completion with the restriction endonuclease Hpal and treated with calf intestinal alkaline phosphatase (Boehringer). The phosphatased, HpaI
25 digested plasmid and the 76 by SnaBI/HpaI fragment were ligated together at 16°C overnight. The ligation was diluted 1:4 with distilled water and 2 ~iI. were used to transform competent HB101 cells (BRZ,). Ampicillin resistant transformants were screened by restriction 30 endonuclease digestion analysis with HpaI enzyme. This plasmid, containing two copies of the p~24 mutation of the In2-2 inducible element, was designated pHPH508/GUS.
The promoter fragment from pHPH508/GUS was excised from the plasmid as a 325 by SpeI/NcoI fragment and 35 purified by polyacrylamide geI electrophoresis as above.

WO 93/03160 y ~ ~ ~ ~ ~ ~ 9 6 PGT/US92/06412 The plasmid pSK29 (constructed as described in Example 7) containing the SSP-3-5 gene behind the 35S promoter was likewise digested to completion with Spel and NcoI
restriction endonucleases, to remove the 35S promoter fragment, and treated with calf intestinal alkaline phosphatase (Boehringer). The phosphatased plasmid and the 325 by SpeI/HgaI fragment were ligated together at 16°C overnight. The ligation was diluted 1:4 with distilled water and 2 ESL were used to transform competent HB101 cells (HRL). Ampicillin resistant transformants were screened by restriction digest analysis. The resultant plasmid containing the 325 by promoter insert was designated pHPH508/SK29 (see Figure 13).
GENERATION OF RICE PROTOPhASTS
R3.ce suspension cultures, initiated from anther-derived callus, were maintained by weekly subculture at a 1:4 dilution ratio with fresh liquid N6 medium as described by Chu et al. [Sci sinica 18:659-668]
containing 2 mg/mL 2,4-dichlorophenoxyacetic acid and 3%
(w/v) sucrose, pH 5.8.. Protoplasts were generated from the suspension culture 4 - 6 days after subculture.
Approximately 15g of tissue were mixed with 4 mL/g tissue of enzyme solution [2% (w/v) cellulase "Onozuka'°
RS and 0.5% (w/v) Macerozyme R10, (both from Yakult Honsha, Nishinomiya, Japan), 13% (w/v) mannitol, 0.463 g/L (NHq) 2SOq, 2 . 83 g/L KN03, 0 . 4 g/h KH2POq, 0 .185 g/Z
MgrSOq ~ 7H20, 0 .166 g/I. CaClz ~ 2H20, 3 . 3 mg/Za MnSOq, 1. 5 mg/h ZnSOq . 7H20, 1. 6 mg/h H3B03, 800 ~tg/L KI, pH 5 . 8 (pH
with 0.3M citric acid)] in a sterile petri dish and allowed to sit overnight. The suspension was then , passed through a sterile 90 micron mesh filter rinsing with sufficient K5.8 media [140 mM NaCl, 3.6 mM KC1, , 0.75 mM Na2HPOq~7H20, 5 mM glucose, 125 mM CaCl2, pH 5.8]

WO 93/03160 _ ~ ~ ~ ~ ~ ~ ~ PC'r/US92/064~2 to bring the final volume of filtrate to 100 mL. The solution was transferred to sterile 50 mL Pyrex~ tubes and centrifuged at 500 rpm (50g) for 10 min in a table top IEC swinging bucket centrifuge. The supernatant was removed with a 25 mL pipette and the protoplasts were resuspended in 35 mL of K5.8 media. After 10 min of centrifugation at 500 rpm the supernatant was again removed and the protoplasts were resuspended in 35 mL of N6 medium containing 140 g/L sucrose. After 20 min centrifugation at 500 rpm the protoplasts formed a floating layer and were removed from the top of the tube using disposable plastic pipettes. Two volumes of K5.8 media were added and the final yield (protoplasts/mL) determined using a Fuchs-Rosenthal hemocytometer.
.TRANSFORMATION AND LABELLING OF RICE PROTOPLASTS
One million protoplasts were transformed with 5 ~1g of the plasmid pHPH508/SK29 containing the synthetic storage protein SSP-3-5 gene and a chemically inducible monocot promoter HPH508. Multiple aliquots of the protoplasts (1 million Bells each) were centrifuged gently at 50g (500 rpm) for 5 min in sterile polystyrene Falcon 2054 tubes (12 x 75 mm). The supernatant was discarded and the cells were gently mixed to resuspend , them in the remaining liquid. Five ~.g of transforming DNA in 5 ~lL of TE [20 mM Tris~Cl, 1 mM EDTA, pH 8.0]
were added per million protoplasts. The tubes were shaken gently to disperse the cells in the DNA solution, and 0.1 mL of a solution containing 40~ PEG1540 (Polysciences Inc., Warrington, PA) and 3 mM CaCl2 were ' added. The resulting protoplast cell suspension was mixed gently and incubated at room temperature for 1 min. One mL of K5.8 solution containing 200 ~lglmL
N-(aminocarbonyl)-2-chlorobenzenesulfonamide was then added to dilute out the PEG and to induce the HPH508 promoter. The solution also contained 50 ~Ci/mL
35S-Methionine (New England Nuclear) to label the SSP-3-protein as it was synthesized. Control protoplasts transformed in an identical manner with pHPH508/GUS were 5 used as a measure of protoplast viability. As with the bacterial expression system, Applicants expected the high methionine content of SSP-3-5,protein to cause it to label better than any other proteins in the rice protoplast.
10 The transformed protoplasts were allowed to incubate overnight in the dark at 25-26°C and the following day methionine was added to a final concentration of 1 mM. GUS assays were performed on control tubes and aliquots were removed from sample 15 tubes for analysis by gel electrophoresis over the next 72 hours. Each aliquot was 100 ALL. The aliquots were spun down, washed three times with K5.8 + 1 mM
methionine and resuspended in 50 41L of lysis buffer (50 mM sodium phosphate, pH 7.0, 10 mM ~-mercaptoethanol, 10 20 mM EDTA, 0.1% TritonT" X-100, 0.1~ N-laurylsarcosine] .
The resuspended protoplasts were vortexed for one min and centrifuged at 12,000 rpm in an eppendorf centrifuge for 15 min to remove detrites. Gel loading buffer (2X -Enprotech) was mixed l:l with the protoplast samples and 25 10 to 20 ~iZ were loaded on each lane of a 10 - 20%
Daiichi intermediate gel. The gel buffers were Tris-Tricine SDS (Enprotech) and the gel was run at 150 volts for 1.3 h. Fluorography was performed using EnlightningT~' (New England Nuclear) according to the 30 manufacturer's directions. The gels were dried onto 0.2 micron nitrocellulose sheets (Biorad) and exposed to Kodak X-Omat RP5 film for 30 min to several days. The identity of SSP-3-5 was confirmed by the Rf value and by immunoprecipitation with the anti GST-SSP-3-5 sezum 35 discussed in Example 3.

WO 93/03160 1 L~'~ g ~ PCT/US92/06412 Immunoprecipitations were done by diluting 40 ~iL of lysed protoplasts with 10 volumes (400 ~tL) of TNET
buffer [50 mM Tris~C1, pH ?.5: 150 mM NaCl; 1 mM EDTA;
2% Triton X100]. The sample was spun five min at full speed in an eppendorf centrifuge to remove debrites.
Serum from a rabbit immunized with GST-SSP-3-5 (Example 3) was added (5 ~1L - 20 N.L) and the sample was incubated at 3?°C from one h to overnight. Killed Staphylococcus Aureus (Calbiochem) cells were added [ 5 E1L of a 10%
suspension] and the sample was incubated at 4°C for min to one h. The sample was pelleted through a cushion of TNET buffer + 40% (w/v) sucrose + 0.1% (w/v) sodium dodecyl sulfate by spinning for five min at full speed in an eppendorf centrifuge. The pellet was 15 resuspended and washed three times in TNT buffer [50 mM
.Tris~C1, pH ?.5~ 150 mM NaCl: 1% Triton X100] and .finally washed once with 120 mM Tris~C1, pH 6.8, resuspended in dye buffer (Enprotech) and loaded on an SDS polyacrylamide gel.
These experiments demonstrate that the SSP-3-5 protein is stable in rice cells derived from embryogenic suspension cultures. The pulse chase experiments indicated that the half life for protein degradation is roughly the same for the SSP-3-5 protein as for the background rice proteins.

WO 93/03160 . PC1'/~JS92/06412 L~ '~
1 .N . . L~STIN
(1) GENERAL INFORMATION:
(i) APPLICANTS: Saverio Carl Falco Sharon J. Keeler Janet A. Rice (ii) TITLE OF INVENTION: Synthetic Storage Proteins , with Defined Structure Containing Programmable Levels of Essential Amino Acids far Improvement of the Nutritional Value of Plants (iii) NUMBER OF SEQUENCES: 113 (iv) CORRESPONDENCE ADDRESS:

(A) ADDRESSEE: E.I. du Pont de Nemours and Company (B) STREET: 1007 Market Street (C) CITY: Wilmington (D) STATE: Delaware (E) COUNTRY: USA

(F) ZIP: 19898 ,, (v) COMPUTER READABLE FORM:

(A) MEDIUM TYPE: Floppy Disk (B) COMPUTER: Macintosh (C) OPERATING SYSTEM: Macintosh System, 6.0 (D) SOFTWARE: Microsoft Word, 4.0 (vi) CURRENT APPLICATION
DATA:

(A) APPLICATION NUMBER:

(B) FILING DATE:

(C) CLASSIFICATION:

(vii) PRIOR APPLICATION DATA:

(A) APPLICATION NUM$ER:07/743,006 (B) FILING DATE: 9 August 1991 WO 93/03160 ~ ~ ~ ~ ~ ~ ~ PCT/US92/06412 (viii) ATTORNEY/AGENT INFORMATION:
(A) NAME: Linda Axamethy Floyd (B) REGISTRATION NUMBER: 33,692 (C) REFERENCE/DOCKET NUMBER: HH-1031 (ix) TELECOMMUNICATION INFORMATION:
(A) TELEPHONE: (302) 992-4929 (B) TELEFAX: (302) 892-7949 (C) TELEX: 835420 WO 93/03160 1 4 .~ ~ ~ PGT/US92/06412 ~ I

(2) INFORMATION
FOR
SEQ
ID
NO:1:

(i) SEQUENCE
CHARACTERISTICS:

(A) LENGTH:

amino acids (B) TYPE:
amino acid (C) STRANDEDNESS:
unknown (D) TOPOLOGY:
unknown (ii) MOLECULE
TYPE:
protein (ix) FEATURE:

(A) NAME/KEY:
Protein (B) LOCATION:
1..28 (D) OTHER
INFORMATION:
/label=
name /note=
"(SSP
4)4"

(xi) SEQUENCE
DESCRIPTION:
SEQ
ID
NO:1:

Leu Glu Lys Glu Leu Lys Ala Leu Glu Glu Lys Leu Lys Ala Leu Glu Glu Lys Lys Leu Ala Leu Glu Glu Lys Leu Lys Ala '(2) INFORMATION
FOR
SEQ
ID
N0:2:

(i) SEQUENCE
CHARACTERISTICS:

(A) LENGTH:

amino acids (B) TYPE:
amino acid (C) STRANDEDNESS:
unknown (D) TOPOLOGY:
unknown (ii) MOLECULE
TYPE:
protein (ix) FEATURE:

(A) NAME/KY:
Protein (B) LOCATION:
1..28 (D) OTHER
INFORMATION:
/label=
name /note=
"
(SSP
5) 4"

(xi) SEQUENCE
DESCRIPTION:
SEQ
ID
N0:2:

Met Glu Lys Glu Met Lys Ala Met Glu Glu Lys Met Lys Ala Met Glu Glu Lys Lys l~.et Ala Met Glu Glu Lys Met Lys Ala (2) INFORMATION
FOR
SEQ
ID
N0:3:

(i) SEQUENCE
CHARACTERISTICS:

(A) LENGTH:

amino acids (B) TYPE:
amino acid ., :, , ... . ;:
,.~.:
WO 93/03160 ~ PCT/US92/06412 (C) STRANDEDNESS: unknown (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: protein (ix) FEATURE:

(A) NAME/KEY: Protein (B) LOCATION: 1..28 (D) OTHER INFORMATION: /label= name /notez "(SSP 7)4"

(xi) SEQUENCE DESCRIPTION: SEQ ID N0:3:

Met Glu Lys Leu Lys Ala Met Glu Glu Lys Leu Glu Lys Ala Met Glu Glu Lys Lys Ala Met Glu Glu Lys Leu Lys Ala Leu (2) INFORMATION
FOR
SEQ
ID
N0:9:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 28 amino acids (B) TYPE: amino acid .' (C) STRANDEDNESS: unknown (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: protein (ix) FEATURE:

(A) NAME/KEY: Protein (B) LOCATION: 1..28 (D) OTHER INFORMATION: /label= name /note= ~' (SSP 8) 4"

(xi) SEQUENCE DESCRIPTION: SEQ ID N0:4:

Met Glu Lys Leu Lys Lys Met Glu Glu Lys Leu Met Glu G1u Lys Lys Glu Lys Leu Lys Lys Met Glu Glu Lys Leu Lys Lys 20 2s (2) INFORMATION FOR SEQ ID N0:5:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 28 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: unknown (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: protein WO 93/03160 ~ ~ ~ ~ ~ ~ ~ 10 4 PCT/US92/06412 (ix) FEATURE:
(A) NAME/KEY: Protein (B) LOCATION: 1..28 (D) OTHER INFORMATION: /label= name /note= " (SSP 9) 4"
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:5:
Met Glu Glu Lys Leu Lys Trp Met Glu Glu Lys Leu Lys Trp Met Glu Glu Lys Leu Lys Trp Met Glu Glu Lys Leu Lys Trp (2) INFORMATION FOR SEQ ID N0:6:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 28 amino acids (H) TYPE: amino acid (C) STRANDEDNESS: unknown (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: protein (ix) FEATURE:
(A) NAME/KEY: Protein (H) LOCATION: 1..28 (D) OTHER INFORMATION: /label= name /note= "(SSP 10)4"
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:6:
Met Glu Glu Lys Met Lys Lys Met Glu Glu Lys Met Lys Lys Met Glu 1 5 10 '' 15 Glu Lys Met Lys Lys Met Glu Glu Lys Met Lys Lys 20 25 a (2) INFORMATION FOR SEQ ID N0:7:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 28 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: unknown (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: protein (ix) FEATURE:
(A) NAME/KEY : Protein (B) LOCATION: 1..28 (D) OTHER INFORMATION: /label= name ' /note= "(SSP 11)4"

.. , WO 93/03160 _ ~ ~ ~ ~ ~ ~ ~ PCT/US92/06412 (xi) SEQUENCE DESCRIPTION: SEQ ID N0:7:

MecGlu Lys Met Lys Trp Met Glu Glu Lys Met Lys Trp Met Glu Glu 1 S 10 ~ 15 GluLys Lys Trp Met Glu Glu Lys Met Lys Trp Met (2)INFORMATION
FOR
SEQ
ID
N0:8:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 30 amino acids (H) TYPE: amino acid (C) STRANDEDNESS: unknown (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: protein (ix) FEATURE:

(A) NAME/KEY: Protein (H) LOCATION: 1..30 (D) OTHER INFORMATION: /label= name /note= "CSP 1"

(xi) SEQUENCE DESCRIPTION: SEQ ID N0:8:

MetGlu Glu G1u Leu Lys Lys Lys Leu Glu Glu Leu Lys Lys Trp Lys TrpGlu Leu Lys Lys Lys Leu Glu Glu Leu Lys Lys Lys Glu (2)INFORMATION
FOR
SEQ
ID
N0:9:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 20 base pairs (H) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE:

(A) NAME/KEY: misc feature _ (H) LOCATION: 1..20 (D) OTHER INFORMATION: (product= "synthetic oligonucleotide"

/standard name= "SM

70"

WO 93/03160 ~ PCT/US92/06412 2~~478~ l06 (xi) SEQUENCE DESCRIPTION: SEQ ID N0:9:

(2) INFORMATION
FOR SEQ ID NO:10:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 24 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ' (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE:

(A) NAME/KEY : misc feature _ (B) LOCATION: 1..24 (D) OTHER INFORMATION: /product= "synthetic oligonucleotide"

/standard name= 'SM

71n _ (xi) SEQUENCE DESCRIPTION: SEQ ID N0:10:

(2) INFORMATION
FOR SEQ ID N0:11:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 27 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE:

(A) NAME/KEY : misc feature (B) LOCATION: 1..27 (D) OTHER INFORMATION: /product "synthetic oligonucleotide"

/standard name= ~SM

7S~

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:11:
TTCATCGATA GGC~ACCACA CCCGTCC 27 (2) INFORMATION FOR SEQ ID N0:12:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 27 base pairs WO 93/03160 ~ ~ ~ ~ ~ ~ P~,'I'/US92/06412 (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE:
(A) NAME/KEY: misc_feature (B) LOCATION: 1..27 (D) OTHER INFORMATION: /product= "synthetic oligonucleotide'°
/standard name= "SM
7 9"
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:12:
AATATCGATG CCACGATGCG TCCGGCG ~ 27 (2) INFORMATION FOR SEQ ID N0:13:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 55 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE:
(A) NAME/KEY: misc_feature (B) LOCATION: 1..55 (D) OTHER INFORMATION: /product= "synthetic oligoaucleotide"
/standard name= "SM
81"
(xi) SEQUENCE DESCRIPTION: SEQ ID NO:13:

(2) INFORMATION FOR SEQ ID N0:14:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 55 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE:
(A) NAME/KEY : misc feature WO 93/03160 ~ 13 ~ ~ g g v PCT/US92/A6412 - 108 ..
(B) LOCATION: 1..SS
(D) OTHER INFORMATION: /product= "synthetic oligonucleotide"
/standard name= "SM
8~°' (xi) SEQUENCE DESCRIPTION: SEQ ID N0:14:

(2) INFORMATION FOR SEQ ID N0:15:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 21 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE:
(A) NAME/KEY: misc_feature (B) LOCATION: 1..21 (D) OTHER INFORMATION: /product= "synthetic oligonucleotide"
/standard name= "SM
84"
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:15:

(2) INFORMATION FOR SEQ ID N0:16: , (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 21 base pairs , (B) TYPE: nucleic acid (C)' STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE:
(A) NAME/KEY: misc_feature (B) LOCATION: 1..21 (D) OTHER INFORMATION: /product= "synthetic oligonucleotide"
/standard name= ~SM
85"

WO 93/03160 ~ ~ ~ l3 ~ ~ ~ PGTlUS92/06412 (xi) SEQUENCE DESCRIPTION: SEQ ID N0:16:
ATCGCCTTCA TCTTC ~CTC C 21 (2) INFORMATION FOR SEQ ID N0:17:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 21 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) ( i x ) FEATURE
(A) NAME/KEY: misc_feature (E) LOCATION: 1..21 (D) OTHER INFORMATION: /product= "syr,~thetic oligonucleotide"
/standard name= "SM
82"
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:17:
~.GATGGAGGAG AAGCTGAAGG C 21 (2) INFORMATION FOR SEQ ID N0:18:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 2l base pairs (H) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE:
(A) NAME/KEY: misc feature (B) LOCATION: 1..21 (D) OTHER INFORMATION: /product= »synthetic oligonucleotide"
/standard name= "SM
83" _ (xi) SEQUENCE DESCRIPTION: SEQ ID N0:18:

(2) INFORMATION FOR SEQ ID N0:1~:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 21 base pairs WO 93/03160 , PC'T/LJS92/06412 ~.~~!~7~c~ llo (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE:
(A) NAME/KEY : misc_feature (B) LOCATION: 1..21 (D) OTHER INFORMATION: /products "synthetic oligonucleotide"
/standard name= "SM
86"
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:19:

(2) INFORMATION FOR SEQ ID N0:20:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 21 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) ( ix) FEATURE
(A) NAME/KEY: misc_feature (B) LOCATION: 1..21 (D) OTHER INFORMATION: /product= "synthetic oligonucleotide"
/standard name= "SM
a~" v (xi) SEQUENCE DESCRIPTION: SEQ ID N0:20:
I

(2) INFORMATION FOR SEQ ID No:2l:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 21 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) ( ix) FEATURE
(A) NAME/F~Y : misc feature WO 93/03160 _ 21 I 4 '~ 8 8 PCT/US92/06412 (B) LOCATION: 1..21 (D) OTHER INFORMATION: /product= "synthetic oligonucleotide"
/standard name= °'SM
88"
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:21:

(2) INFORMATION FOR SEQ ID N0:22:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 21 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE:
(A) NAME/KEY : misc_feature (B) LOCATION: 1,.21 (D) OTHER INFORMATION: /product= "synthetic oligonucleotide"
/standard name= "SM
89"
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:22:

(2) INFORMATION FOR SEQ ID N0:23:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 21 base pairs a (B) TYPE: nucleic acid (C) STR.ANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE:
(A) NAME/KEY: misc_feature (B) LOCATION: 1..21 (D) OTHER INFORMATION: /product= "Synthetic oligonueleotide"
/standard name= "SM
90"

WO 93/03160 ~ ~ ~ r~ 112 PCT/US92/06412 (xi) SEQUENCE DESCRIPTION: SEQ ID N0:.23:

(2) INFORMATION FOR SEQ ID N0:24:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 21 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) ( ix ) FEATURE
(A) NAME/KEYv misc_feature (B) LOCATION: 1..21 (D) OTHER INFORMATION: /product= "synthetic oligonucleotide"
/standard name= "SM
91"
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:24:

(2) INFORMATION FOR SEQ ID N0:25:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 21 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ,:
(ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE:
(A) NAME/KEY: misc feature (B) LOCATION: 1..21 (D) OTHER INFORMATION: /product= '°synthetic oligonucleotide"
/standard name= "SM
92"
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:25:

(2) INFORMATION FOR SEQ ID N0:26:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 21 base pairs WO 93/03160 _ ~ 1.~ if ~ $ $ PCT/US92/06412 (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE:
(A) NAME/KEY: misc_feature (H) LOCATION: 1..21 (D) OTHER INFORMATION: /produet= "synthetic oligonucleotide"
/standard name= "SM
93" _ (xi) SEQUENCE DESCRIPTION: SEQ ID N0:28:

(2) INFORMATION FOR SEQ ID N0:27:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 84 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE:
(A) NAME/KEY: misc_feature (B) LOCATION: 1..89 (D) OTHER INFORMATION: /product= "synthetic oligonucleotide"
/standard name= "SM
98" _ s (xi) SEQUENCE DESCRIPTION: SEQ ID N0:27:
GATGGAGGAA AAGCTGAAAG CGATGGAGGA GAAACTCAAG GCTATGGAAG A,AAAGCTTAA 60 (2) INFORMATION FOR SEQ ID N0:28:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 84 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECTJLE TYPE: DNA (genomic) WO 93/03160 ~ ~ ~ ~ ~ ~ ~ 114 pCT/US92/06412 (ix) FEATURE:
(A) NAME/KEY: misc_feature (B) LOCATION: 1..84 (D) OTHER INFORMATION: /product= "synthetic oligonucleotide"
/standard name= "SM
99"
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:28:

(2) INFORMATION FOR SEQ ID N0:29:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 84 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE:
(A) NAME/KEY: misc_feature (B) LOCATION: 1..84 (D) OTHER INFORMATION: /product= "synthetic oligonucleotide"
/standard name "SM
~.~~"
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:29:

AAAGATGGAG GAAAAGCTTA AATG °84 (2) INFORMATION FOR SEQ ID N0:30:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 84 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE:
(A) NAME/KEY: misc_feature (B) LOCATION: 1..84 (D) OTHER INFORMATION: /product= "synthetic oligonucleotide"

WO 93/031b0 ~ ~ ~ ~ PC'T/US92/06412 /standard name= "SM

101"

(xi) SEC~'7ENCE DESCRIPTION: SEQ ID N0:30:

ATCCATTTAA
GCTTTTCCTC
CTACTTTTTG
AGTTTCTCCT
CCATCCATTT
CAGCTTTTCT

TCCATCTTCT
TAAGCTTTTC
CTCC
g4 (2)INFORMATION
FOR
SEQ
ID
N0:31:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 30 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: unknown (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: protein (ix) FEATURE:

(A) NAME/KEY: Protein (B) LOCATION: 1..30 (D) OTHER INFORMATION: /label= name /note= "CSP 2"

(xi) SEQUENCE DESCRIPTION: SEQ ID N0:31:

MetGlu Glu Glu Met Lys Lys Lys Met Glu Glu Met Lys Lys Trp Lys TzpGlu Met Lys Lys Lys Met Glu Glu Met Lys Lys Lys Glu (2)INFORMATION
FOR
SEQ
ID
N0:32:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 160 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (vi) ORIGINAL SOURCE:

(B) STRAIN: E. coli (G) CELL TYPE: DHS alpha (vii) IMMEDIATE SOURCE:

(B) CLONE: C15 (ix) FEATURE:

(A) NAME/KEY: CDS

(B) LOCATION: 2..151 WO 93103150 21 Z ~ '~ g g PCT/US92l06412 (D) OTHER INFORMATION: /function= "synthetic storage protein"
/product= "protein"
/gene= "ssp"
/standard name=
"5.7.7.7.7.7.5"
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:32:

Met Glu Glu Lys Met Lys Ala Met Glu Glu Lys Leu Lys Ala Met , Glu Glu Lys Leu Lys Ala Met Glu Glu Lys Leu Lys Ala Met Glu Glu Lys Leu Lys Ala Met Glu Glu Lys Leu Lys Ala Met Glu Glu Lys Met Lys Ala ;(2) INFORMATION FOR SEQ ID N0:33:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 49 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID N0:33:
Met Glu Glu Lys Met Lys Ala Met Glu C~lu I~ys Leu Lys Ala Met Glu 1 5 10 15 r GluLys Lys Met Glu Glu Lys Leu Lys Ala Met Glu Glu Lys Leu Ala LeuLys Met Glu Lys Leu Lys Ala Met Glu Glu Lys Met Lys Ala Glu Ala (2)INFORMATION FOR SEQ ID N0:34:

(i) SEQUENCE
CHARACTERISTICS:

(A) LENGTH: 16fl base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double _ (D) TOPOLOGY: linear WO 93/03160 ~ ~ ~ ~ ~ ~ ~ PCT/US92/06412 (ii) MOLECULE TYPE: DNA (genomic) (vi) ORIGINAL SOURCE:
(B) STRAIN: E, coli (G) CELL TYPE: DHS alpha (vii) IMMEDIATE SOURCE:
(B) CLONE: C20 (ix) FEATURE:
(A) NAME/KEY : CDS
(B) LOCATION: 2..151 (D) OTHER INFORMATION: /function= "synthetic storage protein"
/product= "protein'°
. /gene= "ssp"
~/standard_name=
"s.7.7.7 7.7.5"
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:34:

Met Glu Glu Lys Met Lys Ala Met Glu Glu Lys Leu Lys Ala Met Glu Glu Lys Leu Lys Ala Met Glu Glu Lys Leu Lys Ala Met Glu Glu Lys Leu Lys Ala Met Glu Glu Lys Leu Lys Ala Met Glu Glu Lys Met AAG GCG TGATAGGTAC CG ' 160 Lys Ala (2) INFORMATION FOR SEQ ID N0:35:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 49 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID N0:3s:
Met Glu Glu Lys Met Lys Ala Met Glu Glu Lys Leu Lys Ala Met Glu 1 s 10 is Glu Lys Leu Lys Ala Met Glu Glu Lys Leu Lys Ala Met Glu Glu Lys zo 2s 30 WO 93/03160 ~ ~ ~ ~ ~ ~ 11$ PCT/US92/06412 Leu Lys Ala Met Glu Glu Lys Leu Lys Ala Met Glu Glu Lys Met Lys Ala (2) INFORMATION
FOR SEQ
ID N0:36:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 139 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (vi) ORIGINAL SOURCE:

(B) STRAIN: E. coli (G) CELL TYPE: DHS alpha (vii) IMMEDIATE SOURCE:

(8) CLONE: C30 (ix) FEATURE:

' (A) NAME/KEY : CDS

(B) LOCATION: 2..130 (D) OTHER INFORMATION: /function= "synthetic storage protein"

/product "protein"

/gene= "ssp"

/standard name~

_ "5.7.7.7 7.5"

(xi) SEQUENCE DESCRIPTION: SEQ ID N0~36:

Met Glu Glu Lys Met Lys Ala Met Glu Glu Lys Leu Lys Ala Met Glu Glu Lys Leu Lys Ala Met Glu Glu Lys Leu Lys Ala Met G1u Glu.

AAr CTG AAG GCG ATG GAA GAG AAG ATG AAG GCG TGATAGGTAC CG 139 Lys Leu Lys Ala Met Glu G1u Lys Met Lys Ala (2) INFORMATION FOR SEQ ID N0:37:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 42 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear WO 93/03160 _ ~ PGT/US92/06412 (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ N0:37:
ID

Met Glu Lys Met Ala Met Glu Glu Lys Lys Ala Met Glu Lys Leu Glu Glu Lys Lys Ala Glu Glu Lys Leu Lys Met Glu Glu Leu Met Ala Lys Leu Lys Ala Met Glu Glu Lys Met Lys Ala (2) INFORMATION FOR SEQ ID N0:38:
(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 97 base pairs (a) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (vi) ORIGINAL SOURCE:

(B) STRAIN: E. coli (G) CELL TYPE: DH5 alpha (vii) IMMEDIATE SOURCE:

(H) CLONE: D16 (ix) FEATURE:

(A) NAME/KEY: CDS

(H) LOCATION: 2..88 (D) OTHER INFORMATION: /functipn= "synthetic storage protein"

/product= "protein"

/gene= "ssp"

/standard name=

'5.5.5.5"

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:38:

C ATG GAG AAG ATG AAG GCG ATG GAG GAG AAG ATG AAG GCG

Met Glu Glu Lys Met Lys Ala Met Glu Glu Lys Met Lys Ala Met GAG GAG AAG
ATG AAG
GCG ATG
GAA GAG
AAG ATG
AAG GCG
TGATAGGTAC

Glu Glu Lys Met Lys Ala Met Glu Glu Lys Met Lys Ala WO 93/03160 ~ ~ "~ 8 $ 12 0 PGT/US92/06412 (2) INFORMATION FOR SEQ ID N0:3.9:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 28 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID N0:39:
Met Glu Glu Lys Met Lys Ala Met Glu Glu Lys Met Lys Ala Met Glu Glu Lys Met Lys Ala Met Glu Glu Lys Met Lys Ala (2) INFORMATION FOR SEQ ID N0:40:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 118 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (vi) ORIGINAL SOURCE:
(B) STRAIN : E . Coli ' (G) CELL TYPE: DH5 alpha (vii) IMMEDIATE SOURCE:
(B) CLONE: D20 (ix) FEATURE:
(A) NAME/K~Y : CDS
(B) LOCATION: 2..109 (D) OTHER INFORMATION: /function= "synthetic storage proteinn /product= "protein"
/gene "ssp"
/standard name "5.5.5.5.5"
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:40:

Met Glu Glu Lys Met Lys Ala Met Glu Glu Lys Met Lys Ala Met Glu Glu Lys Met Lys Ala Met Glu Glu Lys Met Lys Ala Met Glu Glu ~4~ ~.,,'..., . ,. .~ ~ ~I . . : y ~.. ~. : , .. . , ., ." ,.,,.: ..'i -.~.
.'.'. ~ . ..
WO 93103160 - ~ ~ ~ ~' ~ ~ ~ 1'CT/US92/06412 121 ~ ' LysMetLys Ala (2)INFORMATION
FOR
SEQ
ID
N0:91:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 35 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii). MOLECULE TYPE: protein (xi)SEQUENCE DESCRIPTION: SEQ ID N0:41:

MetGluGlu Lys Met Lys Ala Met Glu Glu Lys Ala Met Glu Lys Met 1 5 10 :l5 GluLysMet Lys Ala Met Glu Glu Lys Met Met Glu G:Lu Lys Lys Ala Met Lys Ala (2) INFORMATION FOR SEQ ID N0:42:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 97 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomie) (vi) ORTGINAL SOURCE:
($) STRAIN : E . col i (G) CELL TYPE: DH5 alpha (vii) IMMEDIATE SOURCE:
(B) CLONE: D33 (ix) FEATURE:
(A) NAME/KEY: CDS
(B) LOCATION: 2..$$
(D) OTHER INFORMATION: /unction= "synthetic Storage protein"
/product= "protein"
/gene "ssp"
/standard name=
°'5.5.5.5"

WO 93/03160 ~ p~-T/US92/06412 ~ ( ' ~
~
~
g V

(xi) SEQUENCE
DESCRIPTION:
SEQ
ID
N0:42:

C ATG GAG GAG
AAG ATG AAG GCG
ATG GAG GAG AAG
ATG AAG GCG ATG

Met Glu Glu Lys Met Lys Ala Met Glu Glu Lys Met Lys Ala Met GAG GAG ATG
AAG AAG
GCG
ATG
GAA
GAG
AAG
ATG
AAG
GCG
TGATAGGTAC

Glu Glu Met Lys Lys Ala Met Glu Glu Lys Met Lys Ala (2) INFORMATION
FOR
SEQ
ID
N0:43:

(i) SEQUENCE
CHARACTERISTICS:

(A) LENGTH:

amino acids (B) TYPE:
amino acid (D) TOPOLOGY:
linear (ii) MOLECULE
TYPE:
protein (xi) SEQUENCE
DESCRIPTION:
SEQ
ID
N0:43:

Met Glu Lys Glu Met Lys Ala Met Glu Glu Lys Met Lys Ala Met Glu Glu Zys Lys Met Ala Met Glu Glu Lys Met Zys Ala (2) INFORMATION
FOR
SEQ
ID
N0:44:

(i) SEQUENCE
CHARACTERISTICS:

(A) LENGTH:

base pairs (B) TYPE:
nucleic aeid (C) STRANDEDNESS:
double (D) TOPOLOGY:
linear (ii) MOLECULE
TYPE:
DNA
(genomic) (vi) ORIGINAi.
SOURCE:

(B) STRAIN:
E.
coli (G) CELL
TYPE:

alpha (vii) IMMEDIATE
SOURCE:

(B) ChONE:

(ix) FEATURE:

(A) NAME/KEY:
CDS

(B) LOCATION:
2..151 (D) OTHER
INFORMATION:
/function=
"synthetic storage protein /product=
"protein"

/gene=
"ssp"

WO 93/031511 ~ ~~ ~,~ ~ ~ ,~ PCT/US92/06412 /standard name=
"7.7.7.7 7.7.5,~
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:44:

Met Glu Glu Lys Leu Lys Ala Met Glu Glu Lys Leu Lys Ala Met Glu Glu Lys Leu Lys Ala Met Glu Glu Lys Leu Lys Ala Met Glu Glu Lys Leu Lys A1a Met Glu Glu Lys Leu Lys Ala Met Glu Glu Lys Met Lys Ala (2) INFORMATION FOR SEQ ID N0:45:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 49 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID N0:45:
Met Glu Glu Lys Leu Lys Ala Met Glu Glu Lys Leu Lys Ala Met Glu r Glu Lys Leu Lys Ala Met Glu Glu Lys Leu Lys Ala Met Glu Glu Lys Leu Lys Ala Met Glu Glu Lys Leu Lys Ala Met Glu Glu Lys Met Lys Ala (2) INFORMATION FOR SEQ ID N0:4f:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 97 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear a (ii) MOLECULE TYPE: DNA (genomic) WO 93/03160 21 ~. ~ 7 ~8 8 PCT/US92/06412 (vi) ORIGINAL SOURCE:
(B) STRAIN: E. coli (G) CELL TYPE: DHS alpha (vii) IMMEDIATE SOURCE:
(B) CLONE: 84-H3 4 (ix) FEATURE:
(A) NAME/KEY : CDS
(B) LOCATION: Z..88 (D) OTHER INFORMATION: /function "synthetic storage protein /product "protein"
!gene= "ssp"
/standard name=
"5.5.5.5"
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:46:
C ATG GAG GAG AAG ATG AAG GCG ATG GAG GAG AAG ATG AAG GCG 7~TG 46 Met Glu Glu Lys Met Lys Ala Met Glu Glu Lys Met Lys Ala Met ~Glu Glu Lys Met Lys Ala Met Glu Glu Lys Met Lys Ala (2) INFORMATION FOR SEQ ID N0:47:
(i) sEQvENCE CHARACTERISTICS:
(A) LENGTH: 28 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID N0:47:
Met Glu Glu Lys Met Lps Ala Met Giu Glu Lys Met Lys Ala Met Glu Glu Lys Met Lys Ala Met Glu Glu Lys Met Lys Ala (2) INFORMATION FOR SEQ ID N0:48:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 97 base pairs (B) . TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear WO 93/03160 ~ 1 ~ l~ ~ ~ ~ PC,T/L1S92/06412 lzs (ii) MOLECULE TYPE: DNA (genomic) (vi) ORIGINAL SOURCE:

(B) STRAIN: E. Coli (G) CELL TYPE: DH5 alpha (vii) IMMEDIATE SOURCE:

(B) CLONE: 86-H23 (ix) FEATURE:

(A) NAME/KEY : CDS

(B) LOCATION: 2..88 (D) OTHER INFORMATION: /function= "synthetic storage protein /product= "protein"

/gene= "ssp"

/standard name=

"5.8.8.5"

(xi) SEQUENCE DESCRIPTION: SEQ ID N0:48:

Met Glu Glu Lys Met Lys Ala Met Glu Glu Lys Leu Lys Lys Met Glu Glu Lys Leu Lys Lys Met Glu Glu Lys Met Lys Ala (2) INFORMATION FOR SEQ ID N0:49:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 28 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear ' (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID N0:49:
Met Glu Glu Lys Met Lys Ala Met Glu Glu Lys Leu Lys Lys Met Glu Glu Lys Leu Lys Lys Met Glu Glu Lys Met Lys Ala (2) INFORMATION FOR SEQ ID N0:50:
(i) SEQUENCE CHARACTERISTICS:
(A) LEN~-:.'~i: 112 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double ~ ~ ~ '~ $ ~

(D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (vi) ORIGINAL SOURCE:

(B) STRAIN: E. Coli (G) CELL TYPE: DH5 alpha , (vii) IMMEDIATE SOURCE:

(B) CLONE: 88-2 (ix) FEATURE:

(A) NAME/KEY: CDS

(B) LOCATION: 2..103 (D) OTHER INFORMATION: /function= "synthetic storage protein /product= "protein' /gene= 'ssp"

/standard name==

"5.9.9.95"

(xi) SEQUENCE DESCRIPTION: SEQ ID N0:50:

C ATG GAG GAG
AAG ATG AAG GCG
AAta AAG CTG
AAG TGG ATG GAG

Met Glu Glu Lys Met Lys Ala Lys Lys Leu Lys Trp Met Glu Glu AAG CTG AAG TGG ATG GAG GAG AAG CTG AAG TGG ATG GAA GAG AAG ATG

Lys Leu Lys Trp Met Glu Glu Lys Leu Lys Trp Met Glu Glu Lys Met Lys Ala (2) INFORMATION FOR SEQ ID N0:51:

(i) SEQUENCE CHARACTERISTI~C~: ' (A) LENGTH: 33 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID N0:51:

Met Glu Glu Lys Met Lys Ala Lys Lys Leu Lys Trp Met Glu Glu Lys Leu Lys Trp Met Glu Glu Lys Leu Lys Trp Met Glu Glu Lys Met Lys Ala -WO 93/03160 ~ ~ ~ ~ ~ PGT/US92/06412 127 .
(2) INFORMATION FOR SEQ ID N0:52:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 118 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (vi) ORIGINAL SOURCE:

(B) STRAIN: E. coli (G) CELL TYPE: DH5 alpha (vii)IMMEDIATE SOURCE:

(B) CLONE: 90-H8 (ix) FEATURE:

(A) NAMEJKEY : CDS

(B) LOCATION: 2..109 (D) OTHER INFORMATION: /function= "s~rnthetic storage protean /product= "protein"

/ge.ne= "ssp"

/standard name=

"5.10.10.10.5"

(xi) SEQUENCE DESCRIPTION: SEQ TD N0:52:
C ATG GAG GAG AAG ATG AAG GCG ATG GAG GAG AAG ATG AAG AAG ATG

Met Glu Glu Lys Met Lys Ala Met Glu Glu Lys Met Lys Lys Met 1 5 10 .~ 15 Glu Glu Lys Met Lys Lys Met Glu Glu Lys Met Lys Lys Met Glu Glu Lys Met Lys Ala (2) INFORMATION FOR SEQ ID N0:53:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 35 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein WO 93/03160 . PCT/US92/06412 2 3. ~. ~'~ ~ 8 i2a . ., (xi) SEQUENCE DESCRIPTION: SEQ ID N0:53:

Met Glu Lys Met Lys Ala Met Glu Glu Lys Met Lys Lys Met Glu Glu Glu Lys Lys Lys Met Glu Glu Lys Met Lys Lys Met Glu Glu Lys Met Met Lys Ala (2) INFORMATION
FOR
SEQ
ID
N0:54:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 97 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (vi) ORIGINAL SOURCE:

(B) STRAIN: E. coli (G) CELL TYPE: DH5 alpha (vii) IMMEDIATE SOURCE:

(B) CLONE: 92-2 (ix) FEATURE:

(A) NAME/KEY : CDS

(B) LOCATION: 2..88 (D) OTHER INFORMATION: /funetion= "synthetic storage protein /product= "protein"

:gene= "ssp"

/standard name= a -"5.11.11.
5"

(xi) SEQUENCE DESCRIPTION: SEQ ID N0:54:

Met Glu Glu Lys Met Lys Ala Met Glu Glu Lys Met Lys Trp Met Glu Glu Lys Met Lys Trp Met Glu Glu Lys Met.Lys Ala (2) INFORMATION FOR SEQ ID N0:55:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 28 amino acids WO 93103160 ~ 1 ~ ~ '~ & $ PCT/US92/06412 (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID N0:55:
Met Glu Glu Lys Met Lys Ala Met Glu Glu Lys Met Lys Trp Met Glu Glu Lys Met Lys Trp Met Glu Glu Lys Met Lys Ala (2) INFORMATION FOR SEQ ID N0:56:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 243 base pairs (8) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (vi) ORIGINAL SOURCE:
(B) STRAIN: E. coli (G) CELL TYPE: DHS alpha (vii) IMMEDIATE SOURCE:
(B) CLONE : 2-9 (ix) FEATURE:
(A) NAME/KEY: CDS
(B) LOCATION: 2..235 (D) OTHER INFORMATION: /function= "synthetic storage protein . /product= "protein"o . /gene "ssp"
/standard name=
"7.7.7.7.?.7.8.9.5.9.5"
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:56:

Met Glu Glu Lys Leu Lys Ala Met Glu Glu Lys Leu Lys Ala Met Glu Glu Lys Leu Lys AIa Met Glu Glu Lys Leu Lys Ala Met Glu Glu AAG CTG A~G GCG ATG GAS GAG AAG CTG AAG GCG ATG GAG GAA AAG CTT 142 Lys Leu Lys AIa Met Glu Glu Lys Leu Lys AIa Met Glu Glu Lys Leu WO 93/03160 2 ~ ~ ~ rl g g PCT/US92/06412 Lys Lys Met Glu Glu Lys Leu Lys Trp Met Glu Glu Lys Leu Lys Lys Met Glu Glu Lys Leu Lys Trp Met Glu Glu Lys Met Lys Ala (2) INFORMATION FOR -SEQ ID N0:57:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 77 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID N0:57:

Met Glu Glu Lys Leu Lys Ala Met Glu Glu Lys Ala Met Glu Lys Leu .Glu Lys Leu Lys Ala Met Glu Glu Lys Leu Met Glu Glu Lys Lys Ala Leu Lys Ala Met Glu Glu Lys Leu Lys Ala Glu Lys Leu Lys Met Glu Lys Met Glu Glu Lys Leu Lys Tzp Met Glu Leu Lys Lys Met Glu Lys Glu Glu Lys Leu Lys Trp Met Glu Glu I~ys Ala Met Lys (2) INFORMATION FOR
SEQ ID N0:58:

s (i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 175 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomie) (vi) ORIGINAL SOURCE:

(B) STRAIN: E. coli (G) CELL TYPE: DH5 alpha (vii) IMMEDIATE SOURCE:

(B) CLONE: s-1 WO 93/03160 ~ ~ v 8~, : . PCT/US92/Ob412 (ix) FEATURE:
(A) NAME/KEY: CDS
(B) LOCATION: 2..172 (D) OTHER INFORMATION: /function= "synthetic storage protein /product= "protein°' /gene= "ssp"
/standard name=
"5.5.5.7 7.7.7.5"
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:58:

Met Glu Glu Lys Met Lys Ala Met Glu Glu Lys Met Lys Ala Met Glu Glu Lys Met Lys Ala Met Glu Glu Lys Leu Lys Ala Met Glu Glu Lys Leu Lys Ala Met Glu Glu Lys Leu Lys Ala Met Glu Glu Lys Leu Lya Ala Met Glu Glu Lys Met Lys Ala (2) INFORMATION FOR SEQ ID N0:59:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGT13: 56 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ZD N0:59:.
MetGluGlu LysMet LysAla Met GluGlu Met AlaMet Lys Lys Glu GluLysMet LysAla MetGlu Glu LysLeu Ala GluGlu Lys Met Lys LeuLysAla MetG1u GluLys Leu LysAla Glu LysLeu Met Glu Lys AlaMetGlu GluLys MetLys Ala (2)INFORMAT ION FORSEQ N0 :60:
ID

a ( i) SEQUEtd:,E CHARACTERISTICS
(A) LENGTH : 7 amino a~:ids 'WO 93/03160 ~ ~ ~ ~ ~ ,Q PC1'/US92/06412 (B) TYPE: amino acid (C) STRANDEDNESS: unknown (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: protein (ix) FEATURE:
(A) NAME/KEY : Protein (B) LOCATION: 1..7 (D) OTHER INFORMATION: /label name /note= "SSP 4"
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:60:
Leu Glu Glu Lys Leu Lys Ala (2) INFORMATION FOR SEQ ID N0:61:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 7 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: unknown (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID N0:61:
Met Glu Glu Lys Leu Lys Ala 1 5 .
(2) INFORMATION FOR SEQ ID N0:62:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: ? amino acids ' (B) TYPE: amino acid (C) STRANDEDNESS: unknown (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID N0:62:
Met Glu Glu Lys Met Lys Ala (2) INFORMATION FOR SEQ ID N0:63:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 7 amino acids (B) TYPE: amino acid WO 93/03160 ~ ~ ~ ~ ~ ~ PGT/US92/Ob412 (C) STRANDEDNESS: unknown (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ~ID N0:63:
Leu Glu Glu Lys Leu Lys Lys (2) INFORMATION FOR SEQ ID N0:~64:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 7 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: unknown (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID N0:64:
Met Glu Glu Lys Leu Lys Lys (2) INFORMATION FOR SEQ ID N0:65:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 7 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: unknown (D) TOPOLOGY: unknown Y.
(ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID N0:65: ' Met Glu Glu Lys Met Lys Lys (2) INFORMATION FOR SEQ ID N0:66:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 7 amino acids (B) TYPE: amino aeid (C) STRANDEDNESS: unknown (D) TOPOLOGY: unkn'~wn (ii) MOLECULE TYPE: protein WO 93103160 ~ ~ ~~ ~ ~ PCT/US92/06412 (xi) SEQUENCE DESCRIPTION: SEQ ID N0:66:
Leu Glu Glu Lys Leu Lys Trp (2) INFORMATION FOR SEQ ID N0:67:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 7 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: unknown (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ TD N0:67:
Met Glu Glu Lys Leu Lys Trp z s (2) INFORMATION FOR SEQ ID N0:68:
(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 7 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: unknown (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID N0:68:

Met Glu Glu Lys Met Lys Trp (2) INFORMATION
FOR SEQ
ID N0:69:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 7 amino acids (B) TYPE: amines acid (C) STRANDEDNESS: unknown (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID N0:69:
Leu Lys Glu Glu Leu Lys Ales WO 93/03160 _ ~ ~ ~ ~, $ PCT/US92/06412 (2) INFORMATION FOR SEQ ID N0:70:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 7 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: unknown (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION:,SEQ ID N0:70:
Met Lys Glu Glu Leu Lys Ala (2)INFORMATION FOR SEQ ID N0:71:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 7 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: unknown (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID N0:71:
Met Lys Glu Glu Met Lys Ala (2) INFORMATION FOR SEQ ID N0:72:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 7 amino acids '(B) TYPE: amino acid (C) STRANDEDNESS: unknown (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID N0:72:
Leu Lys Glu G1u Leu Lys Lys (2)INFORMATION FOR SEQ ID N0:?3:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 7 amino acids 136 .. ., (B) TYPE: amino acid (C) STRANDEDNESS: unknown (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID N0:73:
Met Lys Glu Glu Leu Lys Lys ' (2)INFORMATION FOR SEQ ID N0:79:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 7 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: unknown (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID N0:74:
Met Lys Glu Glu Met Lys Lys (2)INFORMATION FOR SEQ ID N0:75:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 7 amino acids (R) TYPE: amino acid (C) STRANDEDNESS: unknown (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID N0:7S:
Leu Lys Glu Glu Leu Lys Trp (2)INFORMATION FOR SEQ ID N0:76:
(i) SEQUENCE CHARACTERISTICS: .
(A) LENGTH: 7 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: unkno~rn (D) TOPOLOGY: unknown WO 93/03160 ~ ~ ~ ~ ~ ~ ~ PGT/US92/06412 , 137 -(ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID N0:76:
Met Lys Glu Glu Leu Lys Tzp (2)INFORMATION FOR SEQ ID NO:77:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 7 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: unknown (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID N0:77:
Met Lys Glu Glu Met Lys Trp (2) INFORMATION FOR SEQ ID N0:7$:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 7 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: unknown (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: protein (xi} SEQUENCE DESCRIPTION: SEQ ID N0:78:
Met Glu.Asp Lys Met Lys Trp (2) INFORMATION FOR SEQ ID N0:79:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 7 amino aeids (B) TYPE; amino acid (C) STRANDEDNESS: unknown (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: protein .~
WO 93/03160~ ~ ~ c~ ,:, ~ ~ PCT/US92/0641,2 (xi) SEQUENCE DESCRIPTION: SEQ ID N0:79:

Leu Lys Glu Glu Met Ala Lys (2) INFORMATION FOR SEQ ID N0:$0:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 7 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: unknown (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:$0:

Leu Lys Glu Glu Met Lys Lys (2) INFORMATION FOR SEQ ID N0:$1:

(i) SEQUENCE CHARACTERISTICS:

. (A) LENGTH: 7 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: unknown (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID N0:81:

Leu Glu G1u Lys Met Lys Val (2) INFORMATION FOR SEQ ID N0:$2:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 7 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: unknown (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID N0:82:

Met Lys Asp Glu Met Trp Lys ~i~~r'~~8 V~JO 93/03160 P4.'T/L1S92106412 (2) INFORMATION FOR SEQ ID N0:83:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 28 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: unknown (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID N0:83:
Met Glu Glu Lys Leu Lys Lys Met Glu Glu Lys Leu Lys Trp Met Glu Glu Lys Leu Lys Lys Met Glu Glu Lys Leu Lys Trp (2) INFORMATION FOR SEQ ID N0:89:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 134 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (vi) ORIGINAL SOURCE:
(B) STRAIN: E. cola (G) CELL TYPE: DHS alpha (vii) IMMEDIATE SOURCE:
(B) CLONE: segment 3 (seg 3]
(ix) FEATURE:
(A) NAME/KEY: CDS
'(B) LOCATION: 3..116 (D) OTHER INFORMATION: t~unction= 9~synthetic seed storage . protein"
/product= "protein"
/genes "ssp"
lstandard_name=
"SSP-seg3"
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:84:

Met Glu Glu Lys Met Lys Lys Leu Glu Glu Lys Met Lys Ala Met WO 93/03160211 /~ r~ g PCT/US92/06412 g GAG GAC AAG ATG TGGCTT GAG GAA AAG AAG AAG CTC GAA GAG

Glu Asp Lys Met TrpLeu Glu Glu Lys Lys Lys Leu Glu Glu Lys Met ATG

Lys Met Lys Val Lys Met (2) INFORMATION FORSEQ ID N0:85:

(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 37 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID N0:85:
Met Glu Lys Met Lys Lys Leu Glu Glu Lys Met Lys Ala Glu Met Glu Asp Lys Lys Trp Leu Glu Glu Lys Met Lys Lys Leu Glu Met Glu Lys 'Met Lya Met Lys Val (2) INFOF.MATION
FOR SEQ
ID N0:8fi:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 233 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) s (vi) ORIGINAL SOURCE:

('8) STRAIN: E, coli (G) CELL TYPE: DH5 alpha (vii) IMMEDIATE SOURCE:

(B) CLONE: segment 34 [seg 34]

( ix ) FEATURE :

(A) NAME/KEY: CDS

(B) LOCATION: 3..221 (D) OTHER INFORMATION: /function= "synthetic storage protein /product= "protein"

/genes "ssp"

/standard name=

"SSP-seg34"

WO 93/03160 ~ ~ ~ ~ ~ ~ ~ PCT/US92/06412 (xi) SEQUENCE DESCRIPTION: SEQ ID N0:86:

CC
ATG
GAG
GAG
AAG
ATG
AAA
AAG
CTG
GAA
GAA
AAG
ATG
AAG
GCT
ATG

Met Glu Glu Lys Met Lys Lys Leu Glu Glu Lys Met Lys Ala Met GAG GAG ATG AAA TGG CTT GAG GAA AAG AAG GAA GAG

Glu Glu Met Lys Trp Leu Glu Glu Lys Lys Glu Glu Lys Met Lys Leu AAG ATG GTC ATG GAG GAG AAG ATG AAA GAA AAG ATG

Lys Met Val Met Glu Glu Lys Met Lys Glu Lys Met Lys Lys Leu Glu AAG GCA GAA GAC AAA ATG AAG TGG CTT AAA AAG AAG

Lys Ala Glu Asp Lys Met Lys Trp Leu Lys Lys Lys Met Glu Glu Met GAG CGAAT

Leu Glu Lys Met Lys Val Met Lys Glu (2) INFORMATION
FOR
SEQ
ID
N0:87:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 72 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xij SEQUENCE DESCRIPTION: SEQ ID N0:87:

MetGluGlu Lys Lys Lys Leu Glu Lys Lys Ala Met Met Glu Met Glu GluLysMet Lys Leu Glu Glu Met Lys Leu Glu Glu Trp Lys Lys Lys ' MetLysVal Met Glu Lys Met Lys Leu Glu Lys Met Glu Lys Glu Lys AlaMetGlu Asp Met Lys Trp Glu Glu Met Lys Lys Lys Leu Lys Leu GluGluLys Met Val Met Lys Lys (2)INFORMATION FOR SEQ ID
N0:88:

(i) SEQUENCE
CHARACTERISTICS:

(A) LENGTH: 84 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single WO 93/03160 ~ ~ ~ ~ r' ~ ~ PGTlUS92/06412 l 192 (D) TCPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE:
(A) NAME/KEY : misc_featu:~e (B) LOCATION: 1..84 (D) OTHER INFORMATION: /product= "synthetic oligonucleotide"
/standard name= "SM
95"
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:88:

(2) INFORMATION FOR SEQ ID N0:89:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 84 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) ( ix ) FEATURE
(A) NAME/KEY: misc feature (B) LOCATION: 1..84 (D) OTHER INFORMATION: /product= "synthetic oligonucleotide"
/standard name= "SM
97"
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:89:

TCCATCGCCT TCATCTTTTC CTCC $9 (2) INFORMATION FOR SEQ ID N0:90:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 187 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) WO 93/03160 ~ ~ ~ ~ '~ g $ PC~'/iJS9Z/06412 (vi) ORIGINAL SOURCE:
(B) STRAIN: E. coli (G) CELL TYPE: DHS alpha (ix) FEATURE:
(A) NAME/KEY : CDS
(B) LOCATION: 3..173 (D) OTHER INFORMATION: /function= "synthetic storage protein /product "protein"
/gene= "ssp"
/standard name "SSP-3-5"
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:90:

Met Glu Glu Lys Leu Lys Ala Met Glu Glu Lys Leu Lys Ala Met GAG GAG AAG CTG AAG GCG ATG GAG GAG AAG CTG AAG GCG ATG G;AG GAG 95 Glu Glu Lys Leu Lys Ala Met Glu Glu Lys Leu Lys Ala Met Glu Glu ~AAG CTG AAG GCG ATG GAG GAG AAG CTG AAG GCG ATG GAG GAA AAG ATG 143 Lyn I~eu Lya Ala Met Glu Giu Lys Leu Lys Ala Met Glu Glu Lys Met Lys Ala Met Glu Glu Lys Met Lys Ala (2) INFORMATION FOR SEQ ID N0:91:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 56 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID N0:91:
MetGlu GluLys LeuLys AlaMet GluGlu Lys Leu Ala Met Lys Glu 1 s to is GluLys LeuLys AlaMet GluGlu LysLeu Lys Ala Glu Glu Met Lys LeuLys AlaMet GluGlu LysLeu LysAla Met Glu Lys Met Glu Lys AlaMet GluGlu ~ysMet LysAla WO 93/03160 ~ ~ ~ PCT/US92/06412 (2) INFORMATION FOR SEQ ID N0:92:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 61 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear -(ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE:
(A) NAME/KEY : misc_feature (B) LOCATION: 1..61 (D) OTHER INFORMATION: /productF "s.ynthetic oligonucleotide"
/standard name "SM
107" _ (xi) SEQUENCE DESCRIPTION: SEQ ID NO: g2:

~(2) INFORMATION FOR SEQ ID N0:93:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 61 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE:
(A) NAME/KEY: misc feature dB) LocATION: 1..6i - (D) OTHER INFORMATION: /product= "synthetic oligonucleotide"
/standard name= "SM
106"
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:93:

(2) INFORMATION FOR SEQ ID N0:94:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 63 base pairs (B) TYPE: nucleic acid WO 93/03160 '' ~ ~ '~ $ ~ PCT/US92/06412 (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE:
(A) NAME/KEY : misc_feature (B) LOCATION: 1..63 (D) OTHER INFORMATION: /products "synthetic oligonucleotide"
/standard name= "SM
110"
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:94:

GAA s3 (2) INFORMATION FOR SEQ ID N0:95:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 63 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE:
(A) NAME/KEY: misc_feature (B) LOCATION: 1..63 (D) OTHER INFORMATION: /producrt= "synthetic oligonucleotide"
/standard name= "SM
11 ~."
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:95:

(2) INFORMATION FOR SEQ ID N0:96:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 130 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) Wa 93/03160 ~ ~ ~ ~~ ~ ~ ~ PCT/US92/06412 (vi) ORIGINAL SOURCE:
(B) STRAIN: E. coli (G) CELL TYPE: DHS alpha (ix) FEATURE:
(A) NAME/KEY : CDS
(B) LOCATION: 3..116 .
(D) OTHER INFORMATION: /function= "synthetic storage protein /product "protein" , /gene= "ssp"
/standard name=
"SSP-seg4 °~
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:96:
CC ATG GAG GAG AAG ATG .AAA AAG CTC GAA GAA AAG ATG AAG GCA ATG 47 Met Glu Glu Lys Met Lys Lys Leu Glu Glu Lys Met Lys Ala Met GAA GAC AAA ATG AAG TGG CTT GAG GAG AAA ATG AAG AAG CTC G7~A GAG 95 Glu Asp Lys Met Lys Trp Leu Glu Glu Lys Met Lys Lys Leu G:lu Glu Lys Met Lys val Met Lys (2) INFORMATION FOR SEQ ID NO:9?:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 37 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein a (xi) SEQUENCE DESCRIPTION: SEQ ID N0:9?:
Met Glu Glu Lys Met Lys Lys Leu Glu Glu Lys Met Lys A1a Met Glu Asp Lys Met Lys Trp Leu Glu Glu Lys Met Lys Lys Leu Glu Glu Lys Met Lys Val Met Lys (2) INFORMATION FOR SEQ ID N0:98: , (i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 62 base pairs , (H) TYPE: nucleic acid (C) STRANDEDNESS: single WO 93/03160 ~ ~ ~ ~ ~ ~ ~ . PCT/US92/06412 (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE:
(A) NAME/KEY: misc_feature (B) LOCATION: 1..62 (D) OTHER INFORMATION: /product= "synthetic oligonucletide"
/standard name= "SM
112"
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:98:

(2) INFORMATION FOR SEQ ID N0:99:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 62 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single ' (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE:
(A) NAME/KEY: misc_feature (B) LOCATION: 1..62 (D) OTHER INFORMATION: /product= "synthetic oligonucleotide"
/standard name= "SM
118"
(xi) SEQUENCE DESCRIPTION: SEQ TD N0:99:

(2) INFORMATION FOR SEQ ID N0:100:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 130 base pairs (B). TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (vi) ORIGINAL SOURCE:
(B) STRAIN: E. coli (G) CELL TYPE: DHS alpha (ix) FEATURE:
(A) NAME/KEY : CDS
(B) LOCATION: 3..116 (D) OTHER INFORMATION: /function= "synthetic storage protein /product "protein"
/genes nsspn /standard name=
"SSP-seg5"
(xi) SEQUENCE DESCRIPTION: SEQ ID NO:100:

Met Glu Glu Lys Met Lys Lys Leu Lys Glu Glu Met Ala Lys Met Lys Asp Glu Met Trp Lys Leu Lys Glu Glu Met Lys Lys Leu Czlu Glu ~AACs ATG AAG GTC ATG AAG TGATAGGTAC CGAATTC 130 Lys Met Lya Val Met Lys (2) INFORMATION FOR SEQ ID N0:101:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 37 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID N0:101:
Met Glu Glu Lys Met Lys Lys Leu Lys Glu Glu Met A1a Lys Met Lys Asp Glu Met Trp Lys Leu Lys Glu Glu Met Lys Lys Leu Glu Glu Lys Met Lys Val Met Lys (2) INFORMATION FOR SEQ ID N0:102: .
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 63 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single w ~/

(D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE:

(A) NAME/KEY : misc feature _ (B) LOCATION: 1..63 (D) OTHER INFORMATION: /product= "synthetic oligonucleotide"

/standard name= "SM

114"

(xi) SEQUENCE DESCRIPTION: SEQ ID N0:102:

GCTCAAGGAG GAAATGGCTA
AGATGAAAGA
CGAAATCTGG
AAACTGAAAG
AGGAAATGAA

(2) INFORMATION
FOR
SEQ
ID
N0:103:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 63 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE:

(A) NAME/KEY: mist feature (B) LOCATION: 1..63 (D) OTHER INFORMATION: /product= "synthetic oligonucleotide"

name= "SM
Istandard _ 115"

(xi) SEQUENCE DESCRIPTION: SEQ ID N0:103:

AGCTTCTTCA TTTCCTCTTT
CAGTTTCCAC
ATTTCGTCTT
TCATCTTAGC
CATTTCCTCC

(2) INFORMATION
FOR
SEQ
ID
N0:104:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 340 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear . (ii) MOLECULE TYPE: DNA (genomic) WO 93103160 ~ 1 ~ ' j ~ ~ PG'T/US92/06412 (vi) ORIGINAL SOURCE:

(B) STRAIN: E. coli (G) CELL TYPE: DH5 alpha (vii)IMMEDIATE SOURCE:

(B) CLONE: segment 539 [seg 534]

(ix) FEATURE:

(A) NAME/KEY : CDS

(B) LOCATION: 3..326 (D) OTHER INFORMATION: /function= "synthetic seed storage protein' /product= "protein"

/gene= "ssp"

/standard name=

"SSP-534"

(xi) SEQUENCE DESCRIPTION:
SEQ ID N0:104:

CC ATG GAG GAG AAG ATG AAA AAG CTC AAG GAG GAA ATG GCT AAGi ATG 47 Met Glu Glu Lys Met Lys Lys Leu Lys Glu Glu Met Ala Lys Met AAA GAC~GAA ATG TGG AAA CTG AAA GAG GAA ATG AAG AAG CTC GAA GAG 95 Lys Asp Glu Met Trp Lys Leu Lys Glu Glu Met Lys Lys Leu Glu Glu Lys Met Lys Val Met Glu Glu Lys Met Lys Lys Leu Glu Glu Lys Met Lys Ala Met Glu Asp Lys Met Lys Trp Leu Glu Glu Ly~s Met Lys Lys CTC GAA GAG AAG ATG AAG GTC ATG GAG GAG AAG ATG AAA AAG GTC GAA f239 Leu Glu Glu Lys Met Lys Val Met Glu Glu Lys Met Lys Lys Leu Glu 65 ~ 70 75 Glu Lys Met Lys Ala Met Glu Aap Lys Met Lys Txp Leu Glu Glu Lys Met Lya Lys Leu Glu Glu Lys Met Lys Val Met Lys (2) INFORMATION FOR SEQ ID N0:105:
(i) SEQDENCE CHARACTERISTICS:
(A) LENGTH: 107 amino acids (B) TYPE: amino acid WO 93/03160 rt PCT/US92/06412 ::~~.~~ l8~

(D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID N0:105:

MetGlu Lys Mat Lys Lys Leu Lys Glu Glu Met Ala Lys Glu Met Lys AspGlu Trp Lys Leu Lys Glu Glu Met Lys Lys Leu Glu Met Glu Lys MetLys Met Glu Glu~Lys Met Lys Lys Leu Glu Glu Lys Val Met Lys AlaMet Asp Lys Met Lys Trp Leu Glu Glu Lys Met Lys Glu Lys Leu GluGlu Met Lys Val Met Glu Glu Lys Met Lys Lys Leu Lys Glu Glu LysMet Ala Met Glu Asp Lys Met Lys Trp Leu Glu Glu Lys Lys Met LysLys Glu Glu Lys Met Lys Val Met Lys Leu (2)INFORMATION
FOR
SEQ
ID
FO:106:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 16 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE:

(A) NAME/KEY: misc feature _ (B) LOCATION: 1:x16 (D) OTHER INFORMATION: /functiGn= "PCR

primer"

/product= "synthetic oligonucleotide"

/standard name=

"35S1"

(xi) SE~UENCE DESCRIPTION: SEQ ID N0:106:

TTTGGAGGAG GAC~CG ~ 16 WO 93!03160 PCT/US92/06412 2~1~'~38 152 .
(2) INFORMATTON FOR SEQ ID N0:107:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 16 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear .
(ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE:
(A) NAME/KEY: misc_feature (B) LOCATION: 1..16 (D) OTHER INFORMATION: /function= "PCR
primer"
/product= "synthetic oligonucleotide"
/ standard_name=
"MEH15"
(xi) SEQUENCE DESCRIPTION: SEQ ID NO:10?:

(2) INFORMATION FOR SEQ ID N0:108:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 19 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE:
(A) NAME/KEY: misc feature (B) LocATION: 1..19 (D) OTHER INFORMATION: /function= "PCR
primer"
/product= "synthetic oligonucleotide"
/standard name= "SM
129"
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:108:

(2) INFORMATION FOR SEQ ID N0:109:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 21 base pairs 'WO 93/03160 ~ ~'~ $ ~ PCT/US92/06412 (B> TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE:
(A) NAME/KEY: misc_feature (B) LOCATION: 1..21 (D) OTHER INFORMATION: /function= "PCR
primer"
/product= "synthetic oligonucleotide"
/standard name= "SM
125"
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:109:

(2) INFORMATION FOR SEQ ID N0:110:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 20 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (genomic) (ix) FEATURE:
(A) NAME/KEY: misc_feature (B) LOCATION: 1..20 (D) OTHER INFORMATION: /function= "PCR
primer"
/product= "synthet$c oligonucleotide"
/standard name= "SM
12 6"
(xi) SEQUENCE DESCRIPTION: SEQ ID NO:110:

(2) INFORMATION FOR SEQ ID N0:111:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 14 amino aeids (B) TYPE: amino acid (C) STRANDEDNESS: unknown (D) TOPOLOGY: unknown WO 93/03160 P~.'T/US92/06412 ~~~~i~~~ 159 (ii) MOLECULE TYPE: protein (ix) FEATURE:
(A) NAME/KEY: Protein (B) LOCATION: 1..14 (D) OTHER INFORMATION: /label= name /note= "base gene ( (SSPS) 2] "
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:111:
Met Glu Glu Lys Met Lys Ala Met Glu Glu Lys Met Lys Ala (2) INFORMATION FOR SEQ ID N0:112:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 56 amino acids (B) TYPE: amino acid (C) STRANDEDNESS: unknown (D) TOPOLOGY: unknown (ii) MOLECULE TYPE: protein (ix) FEATURE
(A) NAr'tE/KEY : Protein (B) LOCATION: 1..56 (D) OTHER INFORMATION: /labeh name /note= "SSP-3-(A/E) "
(xi) SEQUENCE DESCRIPTION: SEQ ID N0:112:
Met Glu Giu Lys Leu Lys Ala Met Glu Glu Lys Leu Lys Ala Met Glu a Glu Lys Leu Lys Ala Met Glu Glu Lys Leu Lys Ala Met Glu-Glu Lys zo zs 30 Leu Lys Ala Met Glu Glu Lys Leu Lys Ala Met~Glu Glu Lys Met Lys Glu Met Giu Glu Lys Met Lys Ala.

(2) INFORMATION FOR SEQ ID N0:113:
(i) SEQ1JENCE CHARACTERISTICS:
(A) LENGTH: 16 amino acids ' (B) TYPE: amino acid (C) STRANDEDNESS: unknown (D) TOPOLOGY: unknown WO 93/03160 ~ ~ x( ~ ~1US92/06412 (ii) MOLECULE TYPE: protein (ix) FEATURE:
(A) NAME/KEY: Protein (B) LOCATION: 1..16 (D) OTHER INFORMATION: /label= name /note= "pSIC34 base gene"
(xi) SEQUENCE DESCRTPTION: SEQ ID N0:113:
Met Glu Glu Lys Met Lys Lys Leu Glu Glu Lys Met Lys Val Met Lys r

Claims (14)

What is claimed is:

1. A chimeric gene capable of being expressed in a transformed plant cell comprising a nucleic acid fragment encoding a synthetic polypeptide comprising n heptad units in which the residues of each heptad are designated as d e f g a b c, each heptad being the same or different, wherein:
n is at least 4;
a and d are independently selected from the group consisting of Met, Leu, Val, Ile and Thr;
each a and g is independently selected from the group consisting of Glu, Lys, Arg and Asp, provided that, within the same heptad, when a is Glu or Asp, g is Lys or Arg; and when a is Lys or Arg, g is Glu or Asp; and b, c and f are independently any amino acids except Gly or Pro and at least two amino acids of b, c and f in each heptad are selected from the group consisting of Glu, Lys, Asp, Arg, His, Thr, Ser, Asn, Ala, Gln and Cys operably linked to transcriptional and translational regulatory sequences that are functional in plant cells.

2. A chimeric gene of Claim 1 wherein said nucleic acid fragment encodes a polypeptide wherein:
a and d are independently selected from the group consisting of Met and Leu.

3. A chimeric gene of Claim 1 wherein said nucleic acid fragment encodes a polypeptide wherein each a is Lys and each g is Glu.

4. A chimeric gene of Claim 1 wherein said nucleic acid fragment encodes a polypeptide wherein each a is Glu and each g is Lys.

5. A chimeric gene of Claim 1 wherein said nucleic acid fragment encodes a polypeptide wherein:

b, c and f are selected such that if f is a charged amino acid then b or c carries the opposite charge.

6. A chimeric gene of Claim 1 wherein said nucleic acid fragment encodes a synthetic polypeptide comprising n heptad units in which the residues of each heptad are designated as d e f g a b c, each heptad being the same or different wherein:
n is at least 4;
a and d are independently selected from the group consisting of Met and Leu;
each a and g is independently selected from the group consisting of Glu, or Lys, provided that, within the same heptad, when a is Glu, g is Lys; when a is Lys, g is Glu; and b, c, and f are independently any amino acids except Gly or Pro, at least two amino acids of b, c, and f in each heptad are selected from the group consisting of Glu, Lys, Asp, Arg, His, Thr, Ser, Asn, Ala, Gln, and Cys, and b, c, and f are selected such that if f is a charged amino acid then b or c carries the opposite charge.

7. A chimeric gene of claim 1 wherein said nucleic acid fragment encodes a synthetic polypeptide of n heptads wherein the heptads are selected from the group consisting of:
LEEKLKA (SEQ ID NO:60), MEEKLKA (SEQ ID NO:61), MEEKMKA (SEQ
ID NO:62), LEEKLKK (SEQ ID NO:63), MEEKLKK (SEQ ID NO:64), MEEKMKK (SEQ ID NO:65), LEEKLKW (SEQ ID NO:66), MEEKLKW (SEQ
ID NO:67), LKEELKA (SEQ ID NO:69), MKEELKA (SEQ ID NO:70), MKEEMKA (SEQ ID NO:71), LKEELKK (SEQ ID NO:72), MKEELKK (SEQ
ID NO:73), MKEEMKK (SEQ ID NO:74), LKEELKW (SEQ ID NO:75), MKEELKW (SEQ ID NO:76), MEEKMKW (SEQ ID NO:68), MEDKMKW
(SEQ ID NO:78), LKEEMAK (SEQ ID NO:79), LKEEMKK (SEQ ID NO:80), MKEEMKW (SEQ ID NO:77), LEEKMKV (SEQ ID NO:81) and MKDEMWK
(SEQ ID NO:82).

8. A chimeric gene of Claim 1 wherein said nucleic acid fragment encodes a synthetic polypeptide selected form the group consisting of SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 83 and 105.

9. A chimeric gene of claim 1 further comprising an intracellular localization sequence or a sequence encoding a signal peptide is operably joined to the coding sequences for the synthetic peptide.

10. A chimeric gene of Claim 1 wherein said regulatory sequences are active in seeds.

11. A chimeric gene of Claim 1 wherein said transcriptional and translational regulatory sequences that are functional in plant cells are selected from the group consisting of regulatory sequences for soybean kunitz trypsin inhibitor, glycinin,.beta.-conglycinin, lectin, bean lectin, phaseolin, corn 10 kD zein, 27 kD zein, 19 kD zein, globulin 1, and globulin 2.

12. A chimeric gene of Claim 1 wherein said transformed plant cell is from a plant selected from the group consisting of corn, soybean, Brassica napus, Brassica campistris, Brassica oleracea, tobacco, rice, potato, and wheat.

13. An isolated plant cell transformed with the chimeric gene of Claim 1.

14. A method for increasing the content of essential amino acids in seeds of plants relative to seeds of untransformed plants, comprising:
a. measuring the amino acid content of seeds of a plant to determine an amino acid deficiency in said seeds;
b. synthesizing a chimeric gene of Claim 1, wherein said chimeric gene comprises regulatory sequences active in seeds operably linked to a nucleic acid fragment that encodes the customized synthetic polypeptide of claim 1;
c. transforming a plant cell with the synthesized chimeric gene of step b;
d. regenerating plants from said transformed plant cell of step c to obtain mature plants;
wherein the seeds of the mature plants of step d contain a higher content of essential amino acids than untransformed plants.