CA2115671A1 - Sol-gel encapsulated enzyme - Google Patents

Sol-gel encapsulated enzyme

Info

Publication number
CA2115671A1
CA2115671A1 CA002115671A CA2115671A CA2115671A1 CA 2115671 A1 CA2115671 A1 CA 2115671A1 CA 002115671 A CA002115671 A CA 002115671A CA 2115671 A CA2115671 A CA 2115671A CA 2115671 A1 CA2115671 A1 CA 2115671A1
Authority
CA
Canada
Prior art keywords
biological material
sol
active biological
encapsulated enzyme
mixed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002115671A
Other languages
French (fr)
Other versions
CA2115671C (en
Inventor
Jeffrey I. Zink
Fumito Nishida
Stacey A. Yamanaka
Clinton Nishida
Lisa Ellerby
Bruce S. Dunn
Joan S. Valentine
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California
Original Assignee
Jeffrey I. Zink
Fumito Nishida
Stacey A. Yamanaka
Clinton Nishida
Lisa Ellerby
Bruce S. Dunn
Joan S. Valentine
The Regents Of The University Of California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jeffrey I. Zink, Fumito Nishida, Stacey A. Yamanaka, Clinton Nishida, Lisa Ellerby, Bruce S. Dunn, Joan S. Valentine, The Regents Of The University Of California filed Critical Jeffrey I. Zink
Publication of CA2115671A1 publication Critical patent/CA2115671A1/en
Application granted granted Critical
Publication of CA2115671C publication Critical patent/CA2115671C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/552—Glass or silica
    • C—CHEMISTRY; METALLURGY
    • C03—GLASS; MINERAL OR SLAG WOOL
    • C03C—CHEMICAL COMPOSITION OF GLASSES, GLAZES OR VITREOUS ENAMELS; SURFACE TREATMENT OF GLASS; SURFACE TREATMENT OF FIBRES OR FILAMENTS MADE FROM GLASS, MINERALS OR SLAGS; JOINING GLASS TO GLASS OR OTHER MATERIALS
    • C03C4/00—Compositions for glass with special properties
    • C03C4/0007—Compositions for glass with special properties for biologically-compatible glass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/5436—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand physically entrapped within the solid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Inorganic Chemistry (AREA)
  • Biophysics (AREA)
  • Materials Engineering (AREA)
  • Geochemistry & Mineralogy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

An active biological material encapsulated in a glass is formed using a sol-gel process. A metal alkoxide is mixed with water (12) and exposed to ultrasonic energy at pH <= 2 to form a single phase solution which is then buffered to a pH between about 5 and 7 (17). The buffered solution is then mixed with the active biological material (20) and the resultant gel (21) is aged (22) and dried (24). The dried product is a transparent po-rous glass with substantially all of the added active biological material encapsulated therein (26), the biological material re-taining a high level of activity.
CA002115671A 1991-08-13 1992-08-12 Sol-gel encapsulated enzyme Expired - Lifetime CA2115671C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US07/744,524 1991-08-13
US07/744,524 US5200334A (en) 1991-08-13 1991-08-13 Sol-gel encapsulated enzyme
PCT/US1992/006786 WO1993004196A1 (en) 1991-08-13 1992-08-12 Sol-gel encapsulated enzyme

Publications (2)

Publication Number Publication Date
CA2115671A1 true CA2115671A1 (en) 1993-03-04
CA2115671C CA2115671C (en) 2002-10-29

Family

ID=24993019

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002115671A Expired - Lifetime CA2115671C (en) 1991-08-13 1992-08-12 Sol-gel encapsulated enzyme

Country Status (4)

Country Link
US (1) US5200334A (en)
AU (1) AU2503492A (en)
CA (1) CA2115671C (en)
WO (1) WO1993004196A1 (en)

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US5739020A (en) * 1995-01-10 1998-04-14 Pope; Edward J. A. Encapsulation of animal and microbial cells in an inorganic gel prepared from an organosilicon
US5480582A (en) * 1993-06-30 1996-01-02 Pope; Edward J. A. Process for synthesizing amorphous silica microspheres with fluorescence behavior
US5770076A (en) * 1994-03-07 1998-06-23 The Regents Of The University Of California Micromachined capsules having porous membranes and bulk supports
US5985164A (en) * 1994-03-07 1999-11-16 Regents Of The University Of California Method for forming a filter
US5893974A (en) * 1994-03-07 1999-04-13 Regents Of University Of California Microfabricated capsules for immunological isolation of cell transplants
US5798042A (en) * 1994-03-07 1998-08-25 Regents Of The University Of California Microfabricated filter with specially constructed channel walls, and containment well and capsule constructed with such filters
US5985328A (en) * 1994-03-07 1999-11-16 Regents Of The University Of California Micromachined porous membranes with bulk support
DE4408152A1 (en) * 1994-03-11 1995-09-14 Studiengesellschaft Kohle Mbh Immobilized lipases in hydrophobic sol-gel materials
US5420081A (en) * 1994-03-31 1995-05-30 The Regents Of The University Of California Office Of Technology Transfer Preparation of fullerene/glass composites
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US5591453A (en) * 1994-07-27 1997-01-07 The Trustees Of The University Of Pennsylvania Incorporation of biologically active molecules into bioactive glasses
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Also Published As

Publication number Publication date
US5200334A (en) 1993-04-06
CA2115671C (en) 2002-10-29
AU2503492A (en) 1993-03-16
WO1993004196A1 (en) 1993-03-04

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