CA2118043A1 - Immortalized human cell lines containing exogenous cytochrome p450 genes - Google Patents
Immortalized human cell lines containing exogenous cytochrome p450 genesInfo
- Publication number
- CA2118043A1 CA2118043A1 CA002118043A CA2118043A CA2118043A1 CA 2118043 A1 CA2118043 A1 CA 2118043A1 CA 002118043 A CA002118043 A CA 002118043A CA 2118043 A CA2118043 A CA 2118043A CA 2118043 A1 CA2118043 A1 CA 2118043A1
- Authority
- CA
- Canada
- Prior art keywords
- cell lines
- genes
- human cell
- lines containing
- immortalized human
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108010015742 Cytochrome P-450 Enzyme System Proteins 0.000 title abstract 2
- 210000005260 human cell Anatomy 0.000 title 1
- 210000004027 cell Anatomy 0.000 abstract 3
- 239000002246 antineoplastic agent Substances 0.000 abstract 1
- 210000000424 bronchial epithelial cell Anatomy 0.000 abstract 1
- 231100000357 carcinogen Toxicity 0.000 abstract 1
- 239000003183 carcinogenic agent Substances 0.000 abstract 1
- 231100000599 cytotoxic agent Toxicity 0.000 abstract 1
- 229940127089 cytotoxic agent Drugs 0.000 abstract 1
- 239000002619 cytotoxin Substances 0.000 abstract 1
- 239000003471 mutagenic agent Substances 0.000 abstract 1
- 231100000707 mutagenic chemical Toxicity 0.000 abstract 1
- 231100001221 nontumorigenic Toxicity 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/795—Porphyrin- or corrin-ring-containing peptides
- C07K14/80—Cytochromes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
- C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5011—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing antineoplastic activity
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
- G01N33/5017—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity for testing neoplastic activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/24011—Poxviridae
- C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
- C12N2710/24141—Use of virus, viral particle or viral elements as a vector
- C12N2710/24143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
Abstract
Non-tumorigenic, human bronchial epithelial cell lines are provided wherein the cell lines are capable of expressing cytochrome P450 genes which have been inserted into the cell lines. Also provided are methods and kits for identifying potential mutagens, cytotoxins, carcinogens, and chemotherapeutic agents utilizing these cell lines.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US07/869,818 US5356806A (en) | 1987-06-05 | 1992-04-13 | Immortalized human cell lines containing exogenous cytochrome P450 |
US07/869,818 | 1992-04-13 | ||
PCT/US1993/003336 WO1993021327A1 (en) | 1992-04-13 | 1993-04-09 | Immortalized human cell lines containing exogenous cytochrome p450 genes |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2118043A1 true CA2118043A1 (en) | 1993-10-28 |
CA2118043C CA2118043C (en) | 2001-04-03 |
Family
ID=25354316
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002118043A Expired - Lifetime CA2118043C (en) | 1992-04-13 | 1993-04-09 | Immortalized human cell lines containing exogenous cytochrome p450 genes |
Country Status (9)
Country | Link |
---|---|
US (1) | US5356806A (en) |
EP (1) | EP0640137B1 (en) |
AT (1) | ATE254661T1 (en) |
AU (1) | AU680416B2 (en) |
CA (1) | CA2118043C (en) |
DE (1) | DE69333303T8 (en) |
DK (1) | DK0640137T3 (en) |
ES (1) | ES2211868T3 (en) |
WO (1) | WO1993021327A1 (en) |
Families Citing this family (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5506131A (en) * | 1987-06-05 | 1996-04-09 | The United States Of America As Represented By The Department Of Health And Human Services | Immortalized human cell lines containing exogenous cytochrome P450 genes |
WO1991012317A1 (en) * | 1990-02-09 | 1991-08-22 | Genentech, Inc. | Lung cell line and methods of use |
US6620593B1 (en) * | 1993-07-20 | 2003-09-16 | Sumitomo Chemical Company, Limited | Method for safety evaluation of chemical compound using recombinant yeast expressing human cytochrome P450 |
US5885971A (en) | 1995-03-24 | 1999-03-23 | The Regents Of The University Of California | Gene therapy by secretory gland expression |
US5837693A (en) * | 1995-03-24 | 1998-11-17 | The Regents Of The University Of California | Intravenous hormone polypeptide delivery by salivary gland expression |
US6531455B1 (en) * | 1995-03-24 | 2003-03-11 | The Regents Of The University Of California | Delivery of polynucleotides by secretory gland expression |
US5821049A (en) * | 1996-04-05 | 1998-10-13 | Viratest Carcinogen Monitoring, Ltd | In vitro assay for identification of carcinogens |
US6153429A (en) * | 1996-04-05 | 2000-11-28 | Vera Genics Ltd. | Cell lines useful for in vitro assay for indentification of carcinogens |
US5763180A (en) * | 1996-08-14 | 1998-06-09 | Viratest Carcinogen Monitoring Ltd. | In vitro assay for carcinogens using phenotypic transformation of human cells |
DE19815519A1 (en) | 1998-03-31 | 1999-10-07 | Tacke Windenergie Gmbh | Rotor blade for a wind turbine |
DE10012220A1 (en) * | 2000-03-14 | 2001-09-20 | Johannes Doehmer | Test system comprising cells expressing different cytochrome P450 2D6 alleles used to investigate genetically caused metabolite toxicity and to determine toxic, mutagenic or carcinogenic effect of compounds |
WO2003050297A1 (en) * | 2001-10-31 | 2003-06-19 | Bristol-Myers Squibb Company | Methods of screening for toxicity of test compounds |
US9545422B2 (en) * | 2010-03-01 | 2017-01-17 | The Regents Of The University Of California | Methods of inducing photosensitivity by targeting channelrhodopsin-2 and halorhodopsin to subcellular regions of retinal ganglion cells |
JP2023548746A (en) | 2020-10-13 | 2023-11-21 | クリヤ セラピューティクス インコーポレイテッド | Viral vector constructs for delivering nucleic acids encoding cytokines and their use for treating cancer |
CA3206107A1 (en) | 2021-01-26 | 2022-08-04 | Brian Furmanski | Vector constructs for delivery of nucleic acids encoding therapeutic anti-tnf antibodies and methods of using the same |
EP4301860A1 (en) | 2021-03-04 | 2024-01-10 | Kriya Therapeutics, Inc. | Viral vector constructs incorporating dna for inhibiting toll like receptors and methods of using the same |
WO2023133561A1 (en) | 2022-01-09 | 2023-07-13 | Kriya Therapeutics, Inc. | Vector constructs for delivery of nucleic acids encoding therapeutic anti-igf-1r antibodies and methods of using the same |
WO2024054993A1 (en) | 2022-09-09 | 2024-03-14 | Kriya Therapeutics, Inc. | Vector constructs for delivery of nucleic acids encoding therapeutic anti-ctla4 antibodies and methods of using the same |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4816395A (en) * | 1985-12-19 | 1989-03-28 | Peralta Cancer Research Institute | Method for predicting chemosensitivity of anti-cancer drugs |
US5164313A (en) * | 1987-06-05 | 1992-11-17 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Recombinant vaccinia virus encoding cytochromes P-450 |
US4885238A (en) * | 1987-10-30 | 1989-12-05 | The United States Of America As Represented By The Department Of Health And Human Services | Immortalized human bronchial epitherial mesothelial cell lines |
-
1992
- 1992-04-13 US US07/869,818 patent/US5356806A/en not_active Expired - Lifetime
-
1993
- 1993-04-09 AU AU42816/93A patent/AU680416B2/en not_active Expired
- 1993-04-09 ES ES93912165T patent/ES2211868T3/en not_active Expired - Lifetime
- 1993-04-09 CA CA002118043A patent/CA2118043C/en not_active Expired - Lifetime
- 1993-04-09 DE DE69333303T patent/DE69333303T8/en active Active
- 1993-04-09 EP EP93912165A patent/EP0640137B1/en not_active Expired - Lifetime
- 1993-04-09 DK DK93912165T patent/DK0640137T3/en active
- 1993-04-09 WO PCT/US1993/003336 patent/WO1993021327A1/en active IP Right Grant
- 1993-04-09 AT AT93912165T patent/ATE254661T1/en active
Also Published As
Publication number | Publication date |
---|---|
WO1993021327A1 (en) | 1993-10-28 |
EP0640137A1 (en) | 1995-03-01 |
EP0640137B1 (en) | 2003-11-19 |
US5356806A (en) | 1994-10-18 |
DE69333303T2 (en) | 2004-09-09 |
DE69333303T8 (en) | 2005-07-28 |
DE69333303D1 (en) | 2003-12-24 |
AU680416B2 (en) | 1997-07-31 |
DK0640137T3 (en) | 2004-03-29 |
ES2211868T3 (en) | 2004-07-16 |
ATE254661T1 (en) | 2003-12-15 |
CA2118043C (en) | 2001-04-03 |
AU4281693A (en) | 1993-11-18 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request |