CA2122004A1 - Heparinase gene from flavobacterium heparinum - Google Patents

Heparinase gene from flavobacterium heparinum

Info

Publication number
CA2122004A1
CA2122004A1 CA002122004A CA2122004A CA2122004A1 CA 2122004 A1 CA2122004 A1 CA 2122004A1 CA 002122004 A CA002122004 A CA 002122004A CA 2122004 A CA2122004 A CA 2122004A CA 2122004 A1 CA2122004 A1 CA 2122004A1
Authority
CA
Canada
Prior art keywords
gene
flavobacterium heparinum
heparinase
heparinase gene
orf
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002122004A
Other languages
French (fr)
Other versions
CA2122004C (en
Inventor
Ramnath Sasisekharan
Kelley Moremen
Charles L. Cooney
Joseph J. Zimmermann
Robert S. Langer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Massachusetts Institute of Technology
Original Assignee
Ramnath Sasisekharan
Kelley Moremen
Charles L. Cooney
Joseph J. Zimmermann
Robert S. Langer
Massachusetts Institute Of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ramnath Sasisekharan, Kelley Moremen, Charles L. Cooney, Joseph J. Zimmermann, Robert S. Langer, Massachusetts Institute Of Technology filed Critical Ramnath Sasisekharan
Publication of CA2122004A1 publication Critical patent/CA2122004A1/en
Application granted granted Critical
Publication of CA2122004C publication Critical patent/CA2122004C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Abstract

The cloning of the heparinase gene from Flavobacterium Heparinum using the polymerase chain reaction is described. The Open Reading Frame (ORF) corresponded to 1152 base pairs encoding a precursor protein of MW 43,800 daltons. The amino ac-id sequence reveals a 20-residue leader peptide. The gene was expressed in two expression systems in E. Coli.
CA002122004A 1991-10-23 1992-10-22 Heparinase gene from flavobacterium heparinum Expired - Lifetime CA2122004C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US07/783,706 US5714376A (en) 1991-10-23 1991-10-23 Heparinase gene from flavobacterium heparinum
US783,706 1991-10-23
PCT/US1992/009124 WO1993008289A1 (en) 1991-10-23 1992-10-22 HEPARINASE GENE FROM $i(FLAVOBACTERIUM HEPARINUM)

Publications (2)

Publication Number Publication Date
CA2122004A1 true CA2122004A1 (en) 1993-04-29
CA2122004C CA2122004C (en) 2003-08-19

Family

ID=25130160

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002122004A Expired - Lifetime CA2122004C (en) 1991-10-23 1992-10-22 Heparinase gene from flavobacterium heparinum

Country Status (9)

Country Link
US (2) US5714376A (en)
EP (1) EP0610408B1 (en)
JP (2) JP3880062B2 (en)
AT (1) ATE161578T1 (en)
CA (1) CA2122004C (en)
DE (1) DE69223802T2 (en)
ES (1) ES2111653T3 (en)
GR (1) GR3026205T3 (en)
WO (1) WO1993008289A1 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5681733A (en) * 1994-06-10 1997-10-28 Ibex Technologies Nucleic acid sequences and expression systems for heparinase II and heparinase III derived from Flavobacterium heparinum
EP1552846A3 (en) * 1995-09-29 2008-05-07 BioMarin Pharmaceutical Inc Use of heparinases to decrease inflammatory responses
WO1997016556A1 (en) * 1995-10-30 1997-05-09 Massachusetts Institute Of Technology Rationally designed polysaccharide lyases derived from heparinase i
JP2003527822A (en) * 1998-08-27 2003-09-24 マサチューセッツ インスティテュート オブ テクノロジー Rationally designed heparinases from heparinases I and II
US7056504B1 (en) 1998-08-27 2006-06-06 Massachusetts Institute Of Technology Rationally designed heparinases derived from heparinase I and II
US6235725B1 (en) 1998-10-30 2001-05-22 Baker Norton Pharmaceuticals, Inc. Methods and compositions for the prevention of tolerance to medications
CA2370539C (en) 1999-04-23 2009-01-06 Massachusetts Institute Of Technology System and method for notating polymers
JP2003525946A (en) * 2000-03-08 2003-09-02 マサチューセッツ インスティテュート オブ テクノロジー Heparinase III and uses thereof
CA2422059C (en) * 2000-09-12 2012-05-15 Massachusetts Institute Of Technology Methods and products related to low molecular weight heparin
CA2423469A1 (en) * 2000-10-18 2002-04-25 Massachusetts Institute Of Technology Methods and products related to pulmonary delivery of polysaccharides
ATE481478T1 (en) * 2002-06-03 2010-10-15 Massachusetts Inst Technology RATIONALLY DESIGNED LYASES DERIVED FROM CHONDROITINASE B
DE102005038418A1 (en) * 2005-08-12 2007-02-15 Dade Behring Marburg Gmbh Factor Xa-based heparin assay using a heparin-modifying component
US7691613B2 (en) * 2006-11-03 2010-04-06 Momenta Pharmaceuticals, Inc. Glycosaminoglycan lyase IV and uses thereof
US7691612B2 (en) * 2005-11-03 2010-04-06 Momenta Pharmaceuticals, Inc. Heparan sulfate glycosaminoglycan lyase and uses thereof
US7767420B2 (en) 2005-11-03 2010-08-03 Momenta Pharmaceuticals, Inc. Heparan sulfate glycosaminoglycan lyase and uses thereof
CN103992995B (en) * 2014-06-05 2016-05-04 山东大学 A kind of high expressed water-soluble heparin enzyme I fusion and encoding gene thereof
CN110343691B (en) * 2019-07-24 2023-10-31 宝锐生物科技泰州有限公司 Mutant heparinase I and preparation method and application thereof

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS54107584A (en) * 1978-02-10 1979-08-23 Seikagaku Kogyo Co Ltd Preparation of pure heparinase
IL61201A (en) * 1979-10-05 1984-09-30 Choay Sa Oligosaccharides having no more than 8 saccharide moieties,their obtention from heparin and pharmaceutical compositions containing them
IT1141249B (en) * 1980-02-28 1986-10-01 Italfarmaco Spa PROCEDURE FOR THE PREPARATION OF ENZYMATIC ACTIVITY SUPPLY POLYSACCHARID COPOLYMERS, AND COPOLYERS SO OBTAINED
US4341869A (en) * 1980-08-25 1982-07-27 Massachusetts Institute Of Technology Process for producing heparinase
US4373023A (en) * 1980-10-14 1983-02-08 Massachusetts Institute Of Technology Process for neutralizing heparin
US4757012A (en) * 1983-06-28 1988-07-12 Genentech, Inc. Ascorbic acid intermediates and process enzymes
US4847338A (en) * 1985-03-28 1989-07-11 University Of Iowa Research Foundation Low molecular weight heparin fragments as inhibitors of complement activation
FR2584728B1 (en) * 1985-07-12 1987-11-20 Choay Sa PROCESS FOR THE SULFATION OF GLYCOSAMINOGLYCANS AND THEIR FRAGMENTS
US4839293A (en) * 1986-02-24 1989-06-13 The Trustees Of Columbia University In The City Of New York DNA encoding streptavidin, streptavidin produced therefrom, fused polypeptides which include amino acid sequences present in streptavidin and uses thereof
US4795703A (en) * 1986-03-07 1989-01-03 The Children's Medical Center Corporation Heparin assay
US4885207A (en) * 1987-07-13 1989-12-05 Uop Biocompatible protein or ligand immobilization system
ATE113991T1 (en) * 1988-06-06 1994-11-15 Massachusetts Inst Technology PROCESS FOR PURIFICATION OF HIGH PURITY HEPARINASE IN BULK.
JP2833793B2 (en) * 1989-09-19 1998-12-09 科学技術振興事業団 Plasmid encoding heparinase, heparinase-producing strain carrying this plasmid, and method for producing heparinase

Also Published As

Publication number Publication date
ES2111653T3 (en) 1998-03-16
GR3026205T3 (en) 1998-05-29
EP0610408A1 (en) 1994-08-17
EP0610408B1 (en) 1997-12-29
JP2004057201A (en) 2004-02-26
DE69223802D1 (en) 1998-02-05
DE69223802T2 (en) 1998-05-20
WO1993008289A1 (en) 1993-04-29
CA2122004C (en) 2003-08-19
JPH07503608A (en) 1995-04-20
JP3880062B2 (en) 2007-02-14
US5714376A (en) 1998-02-03
US5830726A (en) 1998-11-03
ATE161578T1 (en) 1998-01-15

Similar Documents

Publication Publication Date Title
CA2122004A1 (en) Heparinase gene from flavobacterium heparinum
CA2085134A1 (en) Gdf-1
CA2263889A1 (en) P-selectin ligand proteins
IL80826A (en) Antigenic proteins from eimeria tenella,their production and their use in the prevention of coccidiosis
CA2177827A1 (en) Novel apoptosis-modulating proteins, dna encoding the proteins and methods of use thereof
EP0258411A4 (en) Dna encoding streptavidin, streptavidin produced therefrom, fused polypeptides which include amino acid sequences present in streptavidin and uses thereof.
WO1990002801A3 (en) Plants transformed with a dna sequence from bacillus thuringiensis lethal to lepidoptera
EP1013760A3 (en) Stable bactericidal/permeabilityincreasing protein products and pharmaceutical compositions containing the same
CA2497857A1 (en) Novel p-selectin ligand protein
CA2153756A1 (en) Transport protein which effects the transport of cationic xenobiotics and/or pharmaceuticals, dna sequences encoding it and their use
CA2102702A1 (en) Vitamin K-Dependent Carboxylase
CA2030073A1 (en) Enantioselective amidases, dna sequences encoding them, method of pre paration, and utilization
CA2112998A1 (en) Polyphenol oxidase genes
CA2238296A1 (en) Ultrathermostable protease genes
WO1995030001A3 (en) Novel p-selectin ligand protein
WO1988009378A3 (en) Oncogene-encoded polypeptide having growth factor activity and methods of production and use thereof
EP1022332A4 (en) Beta-fructofuranosidase and gene thereof
HUT69768A (en) Process for preparation of daunorubicin
AU782261C (en) Novel glycosyltransferase genes
EP0347224A3 (en) Novel glutathione peroxidase gene
IT1263022B (en) INTERLEUCHINA-6 MUTANT WITH IMPROVED BIOLOGICAL ACTIVITY COMPARED TO WILD INTERLEUCHINA-6.
NZ504633A (en) A dna molecule encoding a mutant prepro-neuropeptide y, a mutant signal peptide, and uses thereof
WO1990000179A3 (en) Novel proteins, dna coding for said proteins, and their use
TW342413B (en) Novel toxins isolated from Bracon Hebetor
WO2001049714A3 (en) Nope polypeptides, encoding nucleic acids and methods of use

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry
MKEX Expiry

Effective date: 20121022