CA2122450A1 - Solid phase amplification process - Google Patents

Solid phase amplification process

Info

Publication number
CA2122450A1
CA2122450A1 CA002122450A CA2122450A CA2122450A1 CA 2122450 A1 CA2122450 A1 CA 2122450A1 CA 002122450 A CA002122450 A CA 002122450A CA 2122450 A CA2122450 A CA 2122450A CA 2122450 A1 CA2122450 A1 CA 2122450A1
Authority
CA
Canada
Prior art keywords
amplification
assay
target nucleic
solid phase
amplification process
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002122450A
Other languages
French (fr)
Other versions
CA2122450C (en
Inventor
Charles Phillip Morris
Raymond John Harris
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Diatech Pty Ltd
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2122450A1 publication Critical patent/CA2122450A1/en
Application granted granted Critical
Publication of CA2122450C publication Critical patent/CA2122450C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Abstract

An assay and method of use thereof for amplification and detection of target nucleic acids in patient samples, which is rapid, simple and accurate is described. The assay and method utilizes any one of a variety of amplification methods, in combination with primers immobilized onto either the container containing the patient sample, such as a microtiter welt plate, or a dipstick which is readily immersible into the container. Amplification occurs in the presence of both the first, immobilized primer and a second, labelled primer which hybridizes to the target nucleic acid in the opposite direction to the first. Examples demonstrate the effectiveness of the method in detecting a specific species of mycoplasma, as well as the gene characteristic of cystic fibrosis.
CA002122450A 1991-11-01 1992-10-30 Solid phase amplification process Expired - Fee Related CA2122450C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AUPK922491 1991-11-01
AUPK9224 1991-11-01
PCT/AU1992/000587 WO1993009250A1 (en) 1991-11-01 1992-10-30 Solid phase amplification process

Publications (2)

Publication Number Publication Date
CA2122450A1 true CA2122450A1 (en) 1993-05-13
CA2122450C CA2122450C (en) 2004-07-13

Family

ID=3775791

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002122450A Expired - Fee Related CA2122450C (en) 1991-11-01 1992-10-30 Solid phase amplification process

Country Status (6)

Country Link
US (1) US6017738A (en)
EP (1) EP0672173B1 (en)
BR (1) BR9206705A (en)
CA (1) CA2122450C (en)
DE (1) DE69232753T2 (en)
WO (1) WO1993009250A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114196735A (en) * 2020-09-18 2022-03-18 赛纳生物科技(北京)有限公司 Method for on-chip constant temperature amplification sequencing

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114196735A (en) * 2020-09-18 2022-03-18 赛纳生物科技(北京)有限公司 Method for on-chip constant temperature amplification sequencing

Also Published As

Publication number Publication date
CA2122450C (en) 2004-07-13
DE69232753D1 (en) 2002-10-02
EP0672173A1 (en) 1995-09-20
DE69232753T2 (en) 2003-05-15
EP0672173A4 (en) 1997-06-04
BR9206705A (en) 1995-11-21
EP0672173B1 (en) 2002-08-28
US6017738A (en) 2000-01-25
WO1993009250A1 (en) 1993-05-13

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