CA2146946A1 - Cell Culture Substrates and Methods of Use - Google Patents

Cell Culture Substrates and Methods of Use

Info

Publication number
CA2146946A1
CA2146946A1 CA2146946A CA2146946A CA2146946A1 CA 2146946 A1 CA2146946 A1 CA 2146946A1 CA 2146946 A CA2146946 A CA 2146946A CA 2146946 A CA2146946 A CA 2146946A CA 2146946 A1 CA2146946 A1 CA 2146946A1
Authority
CA
Canada
Prior art keywords
collagen
cell culture
films
dried
culture substrates
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2146946A
Other languages
French (fr)
Other versions
CA2146946C (en
Inventor
Mark S. Swiderek
Frank J. Mannuzza
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
Original Assignee
Mark S. Swiderek
Frank J. Mannuzza
Becton, Dickinson And Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mark S. Swiderek, Frank J. Mannuzza, Becton, Dickinson And Company filed Critical Mark S. Swiderek
Publication of CA2146946A1 publication Critical patent/CA2146946A1/en
Application granted granted Critical
Publication of CA2146946C publication Critical patent/CA2146946C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0679—Cells of the gastro-intestinal tract
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S128/00—Surgery
    • Y10S128/08—Collagen
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Abstract

Cell culture substrates comprising dried films of native fibrillar collagen produced by a method in which collagen fibers are hydrolyzed in acid, solubilized, and reformed as gels on porous surfaces under non-physiologic salt conditions to produce large fibers with the striations characteristic of collagen fibers found in vivo. The gels are collapsed onto the porous surfaces by drawing the interfibril fluid out of the gel through the underside of the porous surface and then dried to form films. Dried collagen films made in this manner retain native fibrillar collagen structure and excellent diffusion characteristics. Native fibrillar collagen films produced according to the methods of the invention are useful as cell culture substrates. They have particularly advantageous properties for growth and differentiation of epithelial cells. This effect is synergistically enhanced by addition of butyric acid as a differentiation inducing agent.
CA002146946A 1994-04-25 1995-04-12 Cell culture substrates and methods of use Expired - Fee Related CA2146946C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US23302894A 1994-04-25 1994-04-25
US08/233,028 1994-04-25

Publications (2)

Publication Number Publication Date
CA2146946A1 true CA2146946A1 (en) 1995-10-26
CA2146946C CA2146946C (en) 1998-12-29

Family

ID=22875594

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002146946A Expired - Fee Related CA2146946C (en) 1994-04-25 1995-04-12 Cell culture substrates and methods of use

Country Status (6)

Country Link
US (4) US5763255A (en)
EP (1) EP0684309B1 (en)
JP (1) JP2815135B2 (en)
AU (1) AU686738B2 (en)
CA (1) CA2146946C (en)
DE (1) DE69506781T2 (en)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5916790A (en) * 1995-03-03 1999-06-29 Metabolex, Inc. Encapsulation compositions, and methods
US5932473A (en) * 1997-09-30 1999-08-03 Becton Dickinson And Company Preparation of a cell culture substrate coated with poly-D-lysine
GB9805519D0 (en) * 1998-03-17 1998-05-13 Zeneca Ltd Processes
US6428978B1 (en) * 1998-05-08 2002-08-06 Cohesion Technologies, Inc. Methods for the production of gelatin and full-length triple helical collagen in recombinant cells
EP2295540A1 (en) * 1998-11-19 2011-03-16 Organogenesis, Inc. Bioengineered tissue constructs and methods for producing and using them
US6458589B1 (en) 2000-04-27 2002-10-01 Geron Corporation Hepatocyte lineage cells derived from pluripotent stem cells
US7282366B2 (en) * 2000-04-27 2007-10-16 Geron Corporation Hepatocytes for therapy and drug screening made from embryonic stem cells
US7473555B2 (en) * 2000-04-27 2009-01-06 Geron Corporation Protocols for making hepatocytes from embryonic stem cells
US7256042B2 (en) * 2000-04-27 2007-08-14 Geron Corporation Process for making hepatocytes from pluripotent stem cells
JP2002142751A (en) * 2000-11-13 2002-05-21 Asahi Techno Glass Corp Collagen-coated cell culture vessel and culture member
CA2388723C (en) * 2001-06-06 2012-10-23 Becton, Dickinson & Company Method of providing a substrate with a ready-to-use, uniformly distributed extracellular matrix
WO2004018657A1 (en) * 2002-08-23 2004-03-04 Kinetana Group Inc. Model epithelial cell cultures
US7608455B2 (en) 2003-08-20 2009-10-27 Becton, Dickinson And Company Modified reconstituted basement membrane composition for assay system
US8513011B2 (en) * 2004-08-26 2013-08-20 Biotech Research Ventures Pte Limited Methods and compositions for culturing cardiomyocyte-like cells
US8030361B2 (en) * 2005-08-18 2011-10-04 Koken Co., Ltd. Cell culture carrier implantable in vivo
US9096826B2 (en) * 2005-11-22 2015-08-04 Covalent Materials Corporation Culture substrate and culture method for undifferentiated cell and undifferentiated cultured cell
US20070231884A1 (en) * 2006-04-04 2007-10-04 Toshiba Ceramics Co., Ltd. Porous body and producing method thereof
US8241908B2 (en) 2006-05-16 2012-08-14 Becton, Dickinson And Company Extracellular matrix coated surface for culturing cells
US8148151B2 (en) * 2006-06-02 2012-04-03 Geron Corporation Differentiation of primate pluripotent cells to hepatocyte-lineage cells
US9428728B2 (en) * 2006-11-21 2016-08-30 Coorstek Kk Carrier for undifferentiated cell culture and subculture method thereof
US8361503B2 (en) * 2007-03-02 2013-01-29 University of Pittsburgh—of the Commonwealth System of Higher Education Extracellular matrix-derived gels and related methods
WO2008153063A1 (en) * 2007-06-11 2008-12-18 Dai Nippon Printing Co., Ltd. Cell culture membrane, cell culture kit, porous material, method of producing cell culture membrane and method of producing porous material
JP5194698B2 (en) * 2007-06-11 2013-05-08 大日本印刷株式会社 Cell culture membrane, cell culture kit and method for producing cell culture membrane
JP5493264B2 (en) * 2007-11-05 2014-05-14 ニプロ株式会社 Collagen base material
US8932858B2 (en) * 2008-03-07 2015-01-13 Corning Incorporated Modified polysaccharide for cell culture and release
JP2010011747A (en) * 2008-07-01 2010-01-21 Canon Inc Cell culture container and cell culture method
US20100178698A1 (en) * 2009-01-12 2010-07-15 Pioneer Hi-Bred International, Inc. Tissue culture device
WO2012024390A2 (en) 2010-08-17 2012-02-23 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Biohybrid composite scaffold
CN103260606A (en) 2010-08-24 2013-08-21 加利福尼亚大学董事会 Compositions and methods for cardiac therapy
ES2609242T3 (en) 2012-06-28 2017-04-19 Wolfgang Parak Biomimetic cell culture support
WO2015095380A1 (en) * 2013-12-17 2015-06-25 Kassab Ghassan S Devices, systems and methods for tissue engineering of luminal grafts
EP3119448B1 (en) 2014-03-21 2020-04-22 University of Pittsburgh- Of the Commonwealth System of Higher Education Methods for preparation of a terminally sterilized hydrogel derived from extracellular matrix
CN113403250A (en) * 2021-05-14 2021-09-17 华南理工大学 Bionic collagen membrane-coated tablet and preparation method thereof
WO2022255251A1 (en) * 2021-06-01 2022-12-08 学校法人早稲田大学 Bilayer culture substrate

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2920000A (en) * 1955-11-07 1960-01-05 Ethicon Inc Ethicon Inc Removable valve Collagen article and the manufacture thereof
US2934446A (en) * 1955-12-21 1960-04-26 United Shoe Machinery Corp Collagen fiber masses and methods of making the same
US3014024A (en) * 1958-03-19 1961-12-19 Johnson & Johnson Collagen film
US4172128A (en) * 1975-03-26 1979-10-23 Erhard Thiele Process of degrading and regenerating bone and tooth material and products
US4233360A (en) * 1975-10-22 1980-11-11 Collagen Corporation Non-antigenic collagen and articles of manufacture
US4642292A (en) * 1979-10-29 1987-02-10 Albert Einstein College Of Medicine Of Yeshiva University, A Division Of Yeshiva University Method for isolation of connective tissue biomatrix
US4485096A (en) * 1982-02-26 1984-11-27 Massachusetts Institute Of Technology Tissue-equivalent and method for preparation thereof
US4600533A (en) * 1984-12-24 1986-07-15 Collagen Corporation Collagen membranes for medical use
US4904596A (en) * 1985-08-07 1990-02-27 Fred Hutchinson Cancer Research Center Hybridoma antibody (FH6) defining a human cancer-associated difucoganglioside
US4829000A (en) * 1985-08-30 1989-05-09 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Reconstituted basement membrane complex with biological activity
DE3613575A1 (en) * 1986-04-22 1987-10-29 Linde Ag Method for immobilising microorganisms and/or enzymes
JPH07106146B2 (en) * 1987-04-24 1995-11-15 千代田化工建設株式会社 Animal cell flow culture bed and method for producing the same
US4996154A (en) * 1989-05-04 1991-02-26 Millipore Corporation Method for growing cellular tissue
US5175092A (en) * 1989-05-04 1992-12-29 Millipore Corporation Vitro toxicology kit and method therefor
JPH0785715B2 (en) * 1990-02-14 1995-09-20 三洋化成工業株式会社 Cell culture substrate and cell culture method
US5256418A (en) * 1990-04-06 1993-10-26 Organogenesis, Inc. Collagen constructs
FR2667246A1 (en) * 1990-10-02 1992-04-03 Imedex BIOMATERIAL BASED ON COLLAGEN AND APPLICATIONS.
ATE175242T1 (en) * 1991-02-28 1999-01-15 Anticancer Inc A TISSUE CULTURE PROCEDURE FOR SKIN IN A NATURAL STATE
US5354666A (en) * 1991-08-01 1994-10-11 Thomas Jefferson University Mammalian cell line expressing basement membrane proteins
JPH05252941A (en) * 1991-08-12 1993-10-05 Sakai Enetsukusu Kk Carrier for mammalian cell culture
US5374515A (en) * 1992-11-13 1994-12-20 Organogenesis, Inc. In vitro cornea equivalent model

Also Published As

Publication number Publication date
US5741701A (en) 1998-04-21
AU1644295A (en) 1995-11-02
JPH0838165A (en) 1996-02-13
EP0684309B1 (en) 1998-12-23
US5763255A (en) 1998-06-09
AU686738B2 (en) 1998-02-12
DE69506781D1 (en) 1999-02-04
US5817764A (en) 1998-10-06
CA2146946C (en) 1998-12-29
JP2815135B2 (en) 1998-10-27
EP0684309A1 (en) 1995-11-29
DE69506781T2 (en) 1999-05-20
US5731417A (en) 1998-03-24

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