CA2169315A1 - Primers and probes for the detection of hiv - Google Patents

Primers and probes for the detection of hiv

Info

Publication number
CA2169315A1
CA2169315A1 CA002169315A CA2169315A CA2169315A1 CA 2169315 A1 CA2169315 A1 CA 2169315A1 CA 002169315 A CA002169315 A CA 002169315A CA 2169315 A CA2169315 A CA 2169315A CA 2169315 A1 CA2169315 A1 CA 2169315A1
Authority
CA
Canada
Prior art keywords
primers
hiv
detection
probes
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002169315A
Other languages
French (fr)
Other versions
CA2169315C (en
Inventor
Richard Arvis Respess
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of CA2169315A1 publication Critical patent/CA2169315A1/en
Application granted granted Critical
Publication of CA2169315C publication Critical patent/CA2169315C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS

Abstract

The present invention provides improved primers for the polymerase chain reaction (PCR) amplification of a nucleic acid sequence from the pol gene of the human immunodeficiency virus type 1(HIV-1). The invention also provides improved probes for the detection of the nucleic acid amplified using the primers of the invention. The primers and amplification methods of the invention enable the detection of HIV-1 from any of the known subtypes. The probes of the invention enable simple and rapid hybridization detection assays for detecting amplified HIV-1 nucleic acid.
CA002169315A 1995-02-17 1996-02-12 Primers and probes for the detection of hiv Expired - Lifetime CA2169315C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/390,192 US5599662A (en) 1995-02-17 1995-02-17 Oliconucleotide primers and probes for the detection of HIV-1
US390,192 1995-02-17

Publications (2)

Publication Number Publication Date
CA2169315A1 true CA2169315A1 (en) 1996-08-18
CA2169315C CA2169315C (en) 2009-02-03

Family

ID=23541480

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002169315A Expired - Lifetime CA2169315C (en) 1995-02-17 1996-02-12 Primers and probes for the detection of hiv

Country Status (7)

Country Link
US (1) US5599662A (en)
EP (1) EP0727497B1 (en)
JP (1) JP4463884B2 (en)
AT (1) ATE237698T1 (en)
CA (1) CA2169315C (en)
DE (1) DE69627408T2 (en)
ES (1) ES2196100T3 (en)

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IL151635A0 (en) 1996-07-08 2003-04-10 Burstein Technologies Inc Cleavable signal element device for diagnostic applications and assay method
US6342349B1 (en) 1996-07-08 2002-01-29 Burstein Technologies, Inc. Optical disk-based assay devices and methods
US20050214827A1 (en) * 1996-07-08 2005-09-29 Burstein Technologies, Inc. Assay device and method
US6331275B1 (en) 1996-07-08 2001-12-18 Burstein Technologies, Inc. Spatially addressable, cleavable reflective signal elements, assay device and method
DE19644248A1 (en) * 1996-10-24 1998-04-30 Boehringer Mannheim Gmbh Primers and probes for the detection of HIV
CA2222769C (en) * 1997-01-17 2001-06-12 F. Hoffmann-La Roche Ag Primers for the detection of hiv-1
EP0866071B1 (en) * 1997-03-20 2004-10-20 F. Hoffmann-La Roche Ag Modified primers
US5962665A (en) * 1997-06-16 1999-10-05 Abbott Laboratories Nucleic acid primers and probes for detecting HIV-1 and HIV-2
US6001558A (en) * 1997-06-25 1999-12-14 Ortho Clinical Diagnostics, Inc. Amplification and detection of HIV-1 and/or HIV 2
DE1002138T1 (en) 1997-08-08 2001-06-07 Akzo Nobel Nv NUCLEIC ACID SEQUENCES AS A PRIMER AND PROBE FOR AMPLIFYING AND DETECTING ALL HIV-1 SUBTYPES
US6846905B2 (en) 1997-08-15 2005-01-25 Abbott Laboratories Antigen constructs useful in the detection and differentiation of antibodies to HIV
US6635418B2 (en) * 1998-02-13 2003-10-21 Igen International, Inc. Assay methods for nucleic acid in a sample
AU5080699A (en) * 1998-07-21 2000-02-14 Burstein Laboratories, Inc. Optical disc-based assay devices and methods
US7244570B2 (en) * 1998-08-04 2007-07-17 Luminex Molecular Diagnostics, Inc. Methods and compositions for modulating “marginally indiscriminant” hybridizations
DE19850186A1 (en) * 1998-10-30 2000-05-25 Roche Diagnostics Gmbh New primers and probes for the detection of HIV
US6303293B1 (en) * 1999-02-02 2001-10-16 Ortho-Clinical Diagnostics, Inc. Oligonucleotide reverse transcription primers for efficient detection of HIV-1 and HIV-2 and methods of use thereof
WO2000046403A2 (en) * 1999-02-03 2000-08-10 THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OFHEALT H AND HUMAN SERVICES Methods and reagents for molecular detection of hiv-1 groups m, n and o
AU767983B2 (en) 1999-04-09 2003-11-27 Esoterix Genetic Laboratories, Llc Methods for detecting nucleic acids indicative of cancer
WO2000068436A1 (en) * 1999-05-07 2000-11-16 Nationales Zentrum Für Retroviren Detection system for human immunodeficiency virus based on nucleic acid amplification
DE60043789D1 (en) * 1999-07-09 2010-03-18 Gen Probe Inc HIV-1 detection by nucleic acid amplification
US6586177B1 (en) * 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection
US6849403B1 (en) * 1999-09-08 2005-02-01 Exact Sciences Corporation Apparatus and method for drug screening
WO2001036614A2 (en) * 1999-11-16 2001-05-25 Geneart Gmbh Gessellschaft Für Angewandte Biotechnologie The genome of the hiv-1 inter-subtype (c/b') and use thereof
CA2394921A1 (en) * 1999-12-07 2001-06-14 Anthony P. Shuber Supracolonic aerodigestive neoplasm detection
US6919174B1 (en) 1999-12-07 2005-07-19 Exact Sciences Corporation Methods for disease detection
WO2001057245A2 (en) * 2000-02-04 2001-08-09 K.U.Leuven Research & Development Hiv-1 resistance assay
ATE414794T1 (en) * 2000-09-01 2008-12-15 Gen Probe Inc AMPLIFICATION OF HIV-1 SEQUENCES FOR DETECTION OF SEQUENCES ASSOCIATED WITH DRUG RESISTANCE MUTATIONS
US6582920B2 (en) * 2000-09-01 2003-06-24 Gen-Probe Incorporated Amplification of HIV-1 RT sequences for detection of sequences associated with drug-resistance mutations
US6770752B2 (en) 2001-01-09 2004-08-03 Becton, Dickinson And Company Sequences for detection of HIV-1
JP2004537278A (en) 2001-03-05 2004-12-16 ビジブル ジェネティクス インコーポレイテッド Methods and primers for determining HIV-1 mutations
WO2003071252A2 (en) 2002-02-15 2003-08-28 Exact Sciences Corporation Methods for analysis of molecular events
US20050014249A1 (en) * 2003-02-21 2005-01-20 Norbert Staimer Chromatographic analysis on optical bio-discs and methods relating thereto
WO2004094986A2 (en) * 2003-04-16 2004-11-04 Handylab, Inc. System and method for electrochemical detection of biological compounds
US20040259101A1 (en) * 2003-06-20 2004-12-23 Shuber Anthony P. Methods for disease screening
CA2547750C (en) * 2003-12-19 2014-04-08 Gen-Probe Incorporated Compositions, methods and kits for detecting the nucleic acids of hiv-1 and hiv-2
US7888003B2 (en) 2004-09-08 2011-02-15 The United States Of America As Represented By The Department Of Health And Human Services Methods for the detection of HIV-1 antibodies employing polypeptides obtained from gag P6 protein
WO2006031870A2 (en) 2004-09-14 2006-03-23 Argos Therapeutics, Inc. Strain independent amplification of pathogens and vaccines thereto
EP2121984A2 (en) * 2007-03-16 2009-11-25 454 Life Sciences Corporation System and method for detection of hiv drug resistant variants
DE102008025328B4 (en) 2008-05-27 2013-10-17 DRK-Blutspendedienst Baden-Württemberg-Hessen gGmbH Compositions and methods for amplifying HIV by multiplex PCR in multiple genome regions
US20100136516A1 (en) * 2008-12-01 2010-06-03 454 Life Sciences Corporation System and method for detection of HIV integrase variants

Family Cites Families (7)

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Publication number Priority date Publication date Assignee Title
DE3853422T2 (en) * 1988-06-09 1995-10-26 Innogenetics Nv HIV-3 retrovirus and its use.
AU1741692A (en) * 1991-03-13 1992-10-21 Regents Of The University Of California, The Detection of 3'-azido-3'-deoxythymidine resistance
CA2039449A1 (en) * 1991-03-28 1992-09-29 Sharon Cassol Hiv-1 detection primer and method of detection using the same
FR2677039B1 (en) * 1991-05-31 1994-09-09 Cis Bio Int OLIGONUCLEOTIDES FOR USE AS REAGENTS FOR IN VITRO DETECTION OF HIV TYPE RETROVIRUS INFECTIONS AND METHOD FOR DETECTING HIV TYPE RETROVIRUS.
ATE329054T1 (en) * 1991-12-23 2006-06-15 Chiron Corp SETS OF HIV PROBE FOR SOLUTION SANDWICH HYBRIDATION PROCEDURES
EP0890642A3 (en) * 1992-10-06 2000-09-13 Dade Behring Marburg GmbH Retrovirus of the HIV-group and its application
WO1994023069A1 (en) * 1993-03-26 1994-10-13 Gen-Probe Incorporated Detection of human immunodeficiency virus type 1

Also Published As

Publication number Publication date
DE69627408D1 (en) 2003-05-22
DE69627408T2 (en) 2004-03-25
JP4463884B2 (en) 2010-05-19
ATE237698T1 (en) 2003-05-15
ES2196100T3 (en) 2003-12-16
JPH08242898A (en) 1996-09-24
EP0727497B1 (en) 2003-04-16
CA2169315C (en) 2009-02-03
US5599662A (en) 1997-02-04
EP0727497A1 (en) 1996-08-21

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Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20160212