CA2177612A1 - Nucleic acids encoding a house dust mite allergen, der p iii, and uses therefor - Google Patents
Nucleic acids encoding a house dust mite allergen, der p iii, and uses thereforInfo
- Publication number
- CA2177612A1 CA2177612A1 CA002177612A CA2177612A CA2177612A1 CA 2177612 A1 CA2177612 A1 CA 2177612A1 CA 002177612 A CA002177612 A CA 002177612A CA 2177612 A CA2177612 A CA 2177612A CA 2177612 A1 CA2177612 A1 CA 2177612A1
- Authority
- CA
- Canada
- Prior art keywords
- peptide
- der
- iii
- nucleic acid
- amino acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
- C07K14/43531—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from mites
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/08—Antiallergic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Abstract
Isolated nucleic acids encoding an allergen of Dermatophagoides pteronyssinus, Der p III, are disclosed. A cDNA encoding a peptide having a Der p III activity and a predicted molecular weight of about 24,985 daltons is also described. The nucleic acids can be used as probes to detect the presence of Der p III nucleic acid in a sample or for the recombinant production of peptides having an activity of Der p III. Peptides having an activity of Der p III can be used in compositions suitable for pharmaceutical administration or methods of diagnosing sensitivity to house dust mites.
Claims (47)
1. An isolated nucleic acid comprising a nucleotide sequence encoding a peptide having an activity of a house dust mite allergen, Der p III.
2. An isolated nucleic acid of claim 1, which is a cDNA sequence.
3. An isolated nucleic acid of claim 2, wherein the cDNA comprises a nucleotide sequence shown in Figure 1A and 1B (SEQ ID NO:1).
4. An isolated nucleic acid of claim 2, wherein the cDNA comprises the coding region of a nucleotide sequence shown in Figure 1A and 1B (SEQ ID NO:1).
5. An isolated nucleic acid of claim 1, wherein the peptide comprises an amino acid sequence shown in Figure 1A and 1B (SEQ ID NO:2).
6. An isolated nucleic acid of claim 5, wherein the peptide comprises amino acid residues 1-232 of the sequence shown in Figure 1A and 1B (SEQ ID NO:2).
7. An isolated nucleic acid of claim 1, wherein the peptide is at least 50%
homologous with a sequence comprising an amino acid sequence shown in Figure 1A and 1B
(SEQ ID NO:2).
homologous with a sequence comprising an amino acid sequence shown in Figure 1A and 1B
(SEQ ID NO:2).
8. An isolated nucleic acid of claim 1, wherein the peptide is encoded by a nucleic acid which hybridizes under high or low stringency conditions to a nucleic acid which encodes a peptide comprising an amino acid sequence shown in Figure 1A and 1B
(SEQ ID NO:2).
(SEQ ID NO:2).
9. An isolated nucleic acid of claim 1, wherein the peptide is at least about 10-20 amino acid residues in length.
10. An isolated nucleic acid of claim 1, wherein the peptide is at least about 10-16 amino acid residues in length.
11. An isolated DNA comprising a nucleotide sequence encoding a peptide of at least about 10-16 amino acid residues or more in length and having at least about 50%
homology with an amino acid sequence shown in Figure 1A and 1B (SEQ ID NO:2).
homology with an amino acid sequence shown in Figure 1A and 1B (SEQ ID NO:2).
12. A recombinant expression vector comprising the nucleic acid of claim 1.
13. A recombinant expression vector of claim 12, wherein the nucleic acid is cDNA.
14. A recombinant expression vector of claim 13, wherein the cDNA comprises the nucleotide sequence shown in Figure 1A and 1B (SEQ ID NO:1).
15. A host cell transfected with the recombinant expression vector of claim 12 capable of directing the expression of a peptide having an activity of Der p III.
16. A host cell of claim 15 which is a eucaryotic cell.
17. A host cell transfected with the recombinant expression vector of claim 13 capable of directing the expression of a peptide having an activity of Der p III.
18. A host cell of claim 17 which is a eucaryotic cell.
19. A host cell transfected with the recombinant expression vector of claim 14 capable of directing the expression of a peptide having an activity of Der p III.
20. A host cell of claim 19 which is a eucaryotic cell.
21. A method of producing a peptide having an activity of Der p III comprising culturing a host cell of claim 15 in medium to express the peptide and isolating the peptide from the culture.
22. An isolated peptide having an activity of a house dust mite allergen, Der p III, produced by recombinant expression of a nucleic acid of claim 1.
23. An isolated peptide having an activity of a house dust mite allergen, Der p III, produced by recombinant expression of a nucleic acid of claim 3 .
24. An isolated peptide having an activity of a house dust mite allergen, Der p III, produced by recombinant expression of a nucleic acid of claim 4
25 An isolated peptide having an activity of a house dust mite allergen, Der p III, produced by recombinant expression of a DNA of claim 11.
26. An isolated peptide having an activity of a house dust mite allergen, Der p III, produced by chemical synthesis.
27. An isolated peptide of claim 26 which is at least about 10-20 amino acid residues in length.
28. An isolated peptide of claim 26 which is at least about 10-16 amino acid residues in length.
29. A modified peptide having an activity of Der p 111.
30. A modified peptide of claim 29, wherein at least one cysteine residue present in the Der p III amino acid sequence shown in Figure 1A and 1B (SEQ ID NO:2) is replaced by another amino acid.
31. A modified peptide of claim 30, wherein at least one cysteine residue present in the Der p III amino acid sequence shovin in Figure 1A and 1B (SEQ ID NO:2) is replaced by a serine residue.
32. A modified peptide of claim 29, wherein at least one lysine residue is added to either the amino or carboxy terminus or both the amino and carboxy terminus of the peptide.
33. A substantially pure preparation of a peptide having an activity of a house dust mite allergen, Der p III.
34. A composition suitable for pharmaceutical administration comprising at least one peptide having an activity of Der p III and a pharmaceutically acceptable carrier.
35. A composition of claim 34, wherein the peptide comprises an amino acid sequence of Figure 1A and 1B (SEQ ID NO:2).
36. A composition of claim 3 5, wherein the peptide comprises amino acid residues 1-232 of the amino acid sequence shown in Figure 1A and 1B (SEQ ID NO:2).
37. A method of treating sensitivity to a house dust mite allergen in a subject sensitive to the allergen, comprising administering to the subject the composition of claim 34.
38. A method of detecting sensitivity in a subject to a house dust mite allergen, comprising combining a blood sample obtained from the subject with a peptide of claim 22, under conditions appropriate for binding of blood components with the peptide and determining the extent to which such binding occurs.
39. A method of claim 38, wherein the extent to which binding occurs is determined by assessing T cell function, T cell proliferation, B cell function, binding of the protein to antibodies present in the blood or a combination, thereof.
40. An antibody specifically reactive with a peptide of claim 22.
41. An antibody of claim 40 which is a monoclonal antibody.
42. An antibody specifically reactive with a peptide of claim 25.
43. An antibody of claim 42 which is a monoclonal antibody.
44. A T cell clone specifically reactive with a peptide of claim 22.
45. A soluble T cell receptor specifically reactive with a peptide of claim 22.
46. An antibody specifically reactive with the T cell receptor of claim 45.
47. The antibody of claim 46 wherein the antibody is a monoclonal antibody.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/163,919 | 1993-12-08 | ||
| US08/163,919 US6180771B1 (en) | 1993-12-08 | 1993-12-08 | Nucleic acids encoding a house dust mite allergen, Der p III, and uses therefor |
| PCT/US1994/014073 WO1995015976A1 (en) | 1993-12-08 | 1994-12-07 | Nucleic acids encoding a house dust mite allergen, der p iii, and uses therefor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2177612A1 true CA2177612A1 (en) | 1995-06-15 |
| CA2177612C CA2177612C (en) | 2010-02-16 |
Family
ID=22592177
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2177612A Expired - Lifetime CA2177612C (en) | 1993-12-08 | 1994-12-07 | Nucleic acids encoding a house dust mite allergen, der p iii, and uses therefor |
Country Status (12)
| Country | Link |
|---|---|
| US (5) | US6180771B1 (en) |
| EP (1) | EP0733064B1 (en) |
| JP (1) | JPH09510083A (en) |
| KR (1) | KR20040004481A (en) |
| AT (1) | ATE320445T1 (en) |
| AU (1) | AU697491B2 (en) |
| CA (1) | CA2177612C (en) |
| DE (1) | DE69434665T2 (en) |
| ES (1) | ES2260760T3 (en) |
| NO (1) | NO317850B1 (en) |
| NZ (1) | NZ277698A (en) |
| WO (1) | WO1995015976A1 (en) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6180771B1 (en) * | 1993-12-08 | 2001-01-30 | Immulogic Pharmaceutical Corp. | Nucleic acids encoding a house dust mite allergen, Der p III, and uses therefor |
| WO2000032227A2 (en) * | 1998-11-30 | 2000-06-08 | Cytos Biotechnology Ag | Ordered molecular presentation of antigens, method of preparation and use |
| EP1162983A1 (en) * | 1999-02-25 | 2001-12-19 | Tai June Yoo | Vaccine for house dust mite allergen using naked plasmid dna |
| EP1133994A1 (en) * | 2000-03-11 | 2001-09-19 | Biognostik Gesellschaft für biomolekulare Diagnostik mbH | A method for reversing the immunosuppressive effects of the Melanoma Inhibitory Activity "MIA" |
| DE60336902D1 (en) * | 2002-07-19 | 2011-06-09 | Cytos Biotechnology Ag | VACCINE COMPOSITIONS CONTAINING AMYLOID BETA 1-6 ANTIGENARRAYS |
| EP1602720B1 (en) * | 2003-03-05 | 2010-11-03 | Juridical Foundation The Chemo-Sero-Therapeutic Research Institute | Process for producing heterologous protein in e. coli |
| GB0424563D0 (en) * | 2004-11-05 | 2004-12-08 | Novartis Ag | Organic compounds |
| MX2010001779A (en) * | 2007-08-15 | 2010-07-06 | Circassia Ltd | Peptides for desensibilization against allergens. |
| KR101420064B1 (en) * | 2013-12-31 | 2014-07-15 | 경북대학교 산학협력단 | Species-specific primers for the diagnosis of the genus Panonychus mites and uses thereof |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5338663A (en) * | 1990-08-24 | 1994-08-16 | President And Fellows Of Harvard College | Method of identifying inhibitors of β-protein esterase activity |
| US5506097A (en) * | 1990-08-24 | 1996-04-09 | President And Fellows Of Harvard College | Method for inhibiting β-protein enzymatic activity |
| JP3139777B2 (en) * | 1990-08-27 | 2001-03-05 | フマキラー株式会社 | Recombinant mite allergen |
| ATE444360T1 (en) | 1991-10-16 | 2009-10-15 | Merck Patent Gmbh | T-CELL EPITOPES FROM THE MAIN ALLERGENS OF DERMATOPHAGOIDES (HOUSE MITES) |
| US5710126A (en) * | 1993-03-12 | 1998-01-20 | Immulogic Pharmaceutical Corporation | T cell epitopes of ryegrass pollen allergen |
| US6180771B1 (en) | 1993-12-08 | 2001-01-30 | Immulogic Pharmaceutical Corp. | Nucleic acids encoding a house dust mite allergen, Der p III, and uses therefor |
| US20040092446A1 (en) * | 1997-07-23 | 2004-05-13 | Goulmy Elsa A.J.M. | HA-1 epitopes and uses thereof |
-
1993
- 1993-12-08 US US08/163,919 patent/US6180771B1/en not_active Expired - Lifetime
-
1994
- 1994-12-07 AT AT95904273T patent/ATE320445T1/en active
- 1994-12-07 EP EP95904273A patent/EP0733064B1/en not_active Expired - Lifetime
- 1994-12-07 AU AU13027/95A patent/AU697491B2/en not_active Expired
- 1994-12-07 KR KR10-2003-7008889A patent/KR20040004481A/en not_active Application Discontinuation
- 1994-12-07 NZ NZ277698A patent/NZ277698A/en not_active IP Right Cessation
- 1994-12-07 ES ES95904273T patent/ES2260760T3/en not_active Expired - Lifetime
- 1994-12-07 CA CA2177612A patent/CA2177612C/en not_active Expired - Lifetime
- 1994-12-07 JP JP7516316A patent/JPH09510083A/en active Pending
- 1994-12-07 DE DE69434665T patent/DE69434665T2/en not_active Expired - Lifetime
- 1994-12-07 WO PCT/US1994/014073 patent/WO1995015976A1/en active IP Right Grant
-
1995
- 1995-06-05 US US08/462,515 patent/US6752991B1/en not_active Expired - Lifetime
-
1996
- 1996-06-07 NO NO19962407A patent/NO317850B1/en not_active IP Right Cessation
-
2004
- 2004-05-14 US US10/846,341 patent/US7666429B2/en not_active Expired - Fee Related
-
2009
- 2009-01-23 US US12/358,954 patent/US9447154B2/en not_active Expired - Fee Related
-
2010
- 2010-01-15 US US12/688,744 patent/US8287875B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| NO317850B1 (en) | 2004-12-20 |
| ES2260760T3 (en) | 2006-11-01 |
| CA2177612C (en) | 2010-02-16 |
| NO962407D0 (en) | 1996-06-07 |
| KR20040004481A (en) | 2004-01-13 |
| US6752991B1 (en) | 2004-06-22 |
| US20110117116A1 (en) | 2011-05-19 |
| US8287875B2 (en) | 2012-10-16 |
| US20050123569A1 (en) | 2005-06-09 |
| ATE320445T1 (en) | 2006-04-15 |
| EP0733064A1 (en) | 1996-09-25 |
| NO962407L (en) | 1996-06-07 |
| US20110059522A1 (en) | 2011-03-10 |
| US6180771B1 (en) | 2001-01-30 |
| DE69434665T2 (en) | 2007-02-08 |
| AU1302795A (en) | 1995-06-27 |
| JPH09510083A (en) | 1997-10-14 |
| AU697491B2 (en) | 1998-10-08 |
| US9447154B2 (en) | 2016-09-20 |
| DE69434665D1 (en) | 2006-05-11 |
| NZ277698A (en) | 1998-04-27 |
| US7666429B2 (en) | 2010-02-23 |
| WO1995015976A1 (en) | 1995-06-15 |
| EP0733064B1 (en) | 2006-03-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKEX | Expiry |
Effective date: 20141208 |