CA2182517A1 - Ligase/polymerase-mediated primer extension of single nucleotide polymorphisms and its use in genetic analysis - Google Patents

Ligase/polymerase-mediated primer extension of single nucleotide polymorphisms and its use in genetic analysis

Info

Publication number
CA2182517A1
CA2182517A1 CA002182517A CA2182517A CA2182517A1 CA 2182517 A1 CA2182517 A1 CA 2182517A1 CA 002182517 A CA002182517 A CA 002182517A CA 2182517 A CA2182517 A CA 2182517A CA 2182517 A1 CA2182517 A1 CA 2182517A1
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CA
Canada
Prior art keywords
ligase
polymerase
single nucleotide
primer extension
nucleotide polymorphisms
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002182517A
Other languages
French (fr)
Other versions
CA2182517C (en
Inventor
Theo Nikiforov
Jonathan Karn
Philip Goelet
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Beckman Coulter Inc
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Individual
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Publication date
Application filed by Individual filed Critical Individual
Publication of CA2182517A1 publication Critical patent/CA2182517A1/en
Application granted granted Critical
Publication of CA2182517C publication Critical patent/CA2182517C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Abstract

A method is provided for determining the identity of a nucleotide at a preselected site in a nucleic acid molecule. The method involves the incorporation of a nucleoside triphosphate that is complementary to the nucleotide present at the preselected site onto the terminus of a primer molecule, and their subsequent ligation to a second oligonucleotide. The reaction is monitored by detecting a specific label attached to the reaction's solid phase or by detection in solution.
CA002182517A 1994-02-07 1995-02-07 Ligase/polymerase-mediated primer extension of single nucleotide polymorphisms and its use in genetic analysis Expired - Fee Related CA2182517C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US19263194A 1994-02-07 1994-02-07
US08/192,631 1994-02-07
PCT/US1995/001639 WO1995021271A1 (en) 1994-02-07 1995-02-07 Ligase/polymerase-mediated genetic bit analysistm of single nucleotide polymorphisms and its use in genetic analysis

Publications (2)

Publication Number Publication Date
CA2182517A1 true CA2182517A1 (en) 1995-08-10
CA2182517C CA2182517C (en) 2001-08-21

Family

ID=22710442

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002182517A Expired - Fee Related CA2182517C (en) 1994-02-07 1995-02-07 Ligase/polymerase-mediated primer extension of single nucleotide polymorphisms and its use in genetic analysis

Country Status (8)

Country Link
US (2) US5679524A (en)
EP (1) EP0754240B1 (en)
JP (1) JP3175110B2 (en)
AT (1) ATE247716T1 (en)
AU (1) AU694187B2 (en)
CA (1) CA2182517C (en)
DE (1) DE69531542T2 (en)
WO (1) WO1995021271A1 (en)

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EP0754240A1 (en) 1997-01-22
CA2182517C (en) 2001-08-21

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