CA2185239A1 - Isothermal strand displacement nucleic acid amplification - Google Patents

Isothermal strand displacement nucleic acid amplification

Info

Publication number
CA2185239A1
CA2185239A1 CA002185239A CA2185239A CA2185239A1 CA 2185239 A1 CA2185239 A1 CA 2185239A1 CA 002185239 A CA002185239 A CA 002185239A CA 2185239 A CA2185239 A CA 2185239A CA 2185239 A1 CA2185239 A1 CA 2185239A1
Authority
CA
Canada
Prior art keywords
nucleic acid
primers
acid amplification
strand displacement
isothermal strand
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002185239A
Other languages
French (fr)
Other versions
CA2185239C (en
Inventor
Nanibhushan Dattagupta
Paul Douglas Stull
Marc Spingola
Daniel Louis Kacian
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gen Probe Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2185239A1 publication Critical patent/CA2185239A1/en
Application granted granted Critical
Publication of CA2185239C publication Critical patent/CA2185239C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

Abstract

Methods for amplifying target nucleic acid sequences using a nucleic acid polymerase lacking 5' exonuclease activity and a set of oligonucleotide primers. Preferably, a primer array is used. The primer array contains two sets of primers. One set contains at least two complementary primers, The other set contains at least two sense primers. Using the described methods, amplification can be carried out under essentialy constant environmental conditions without the requirement for exonuclease activity or restriction endonuclease activity.
CA002185239A 1994-03-16 1995-03-14 Isothermal strand displacement nucleic acid amplification Expired - Fee Related CA2185239C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US21508194A 1994-03-16 1994-03-16
US08/215,081 1994-03-16
PCT/US1995/003339 WO1995025180A1 (en) 1994-03-16 1995-03-14 Isothermal strand displacement nucleic acid amplification

Publications (2)

Publication Number Publication Date
CA2185239A1 true CA2185239A1 (en) 1995-09-21
CA2185239C CA2185239C (en) 2002-12-17

Family

ID=22801567

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002185239A Expired - Fee Related CA2185239C (en) 1994-03-16 1995-03-14 Isothermal strand displacement nucleic acid amplification

Country Status (11)

Country Link
US (4) US6087133A (en)
EP (1) EP0676476B1 (en)
JP (1) JPH09510351A (en)
KR (1) KR100230718B1 (en)
AT (1) ATE207543T1 (en)
AU (1) AU687535B2 (en)
CA (1) CA2185239C (en)
DE (1) DE69523360T2 (en)
DK (1) DK0676476T3 (en)
ES (1) ES2164112T3 (en)
WO (1) WO1995025180A1 (en)

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