CA2198336A1 - Immunoassay for h. pylori in fecal specimens - Google Patents
Immunoassay for h. pylori in fecal specimensInfo
- Publication number
- CA2198336A1 CA2198336A1 CA002198336A CA2198336A CA2198336A1 CA 2198336 A1 CA2198336 A1 CA 2198336A1 CA 002198336 A CA002198336 A CA 002198336A CA 2198336 A CA2198336 A CA 2198336A CA 2198336 A1 CA2198336 A1 CA 2198336A1
- Authority
- CA
- Canada
- Prior art keywords
- pylori
- antibody
- complex
- antigen
- specimen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
- G01N33/56922—Campylobacter
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/205—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Campylobacter (G)
Abstract
1. A process for the determination of H. pylori in a fecal specimen which comprises:
(a) dispersing a fecal specimen suspected of carrying H. pylori in a sample diluent;
(b) contacting the fecal specimen in the diluent with a first polyclonal antibody for H. pylori antigen to form a complex of the antibody and the antigen;
(c) separating said specimen and said complex;
(d) exposing the complex to a second polyclonal antibody for said antigen and a portion of the antibody reacting with said complex, one of said first and second antibody being bound to a solid carrier and the other being labelled with a detection agent; and (e) determining the amount of the labelled antibody and in turn determining the presence of H. pylori antigen in said fecal specimen.
(a) dispersing a fecal specimen suspected of carrying H. pylori in a sample diluent;
(b) contacting the fecal specimen in the diluent with a first polyclonal antibody for H. pylori antigen to form a complex of the antibody and the antigen;
(c) separating said specimen and said complex;
(d) exposing the complex to a second polyclonal antibody for said antigen and a portion of the antibody reacting with said complex, one of said first and second antibody being bound to a solid carrier and the other being labelled with a detection agent; and (e) determining the amount of the labelled antibody and in turn determining the presence of H. pylori antigen in said fecal specimen.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/647,115 US5716791A (en) | 1996-05-09 | 1996-05-09 | Immunoassay for H. pylori in fecal specimens |
US08/647,115 | 1996-05-09 |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2198336A1 true CA2198336A1 (en) | 1997-11-10 |
CA2198336C CA2198336C (en) | 2002-06-18 |
Family
ID=24595765
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002198336A Expired - Lifetime CA2198336C (en) | 1996-05-09 | 1997-02-24 | Immunoassay for h. pylori in fecal specimens |
Country Status (12)
Country | Link |
---|---|
US (3) | US5716791A (en) |
EP (1) | EP0806667B2 (en) |
JP (1) | JP3043999B2 (en) |
CN (1) | CN1271408C (en) |
AR (1) | AR006016A1 (en) |
AT (1) | ATE211552T1 (en) |
AU (1) | AU698513B2 (en) |
CA (1) | CA2198336C (en) |
DE (2) | DE69709434T3 (en) |
ES (1) | ES2132044T5 (en) |
GR (1) | GR990300022T1 (en) |
MX (1) | MX9701877A (en) |
Families Citing this family (31)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5716791A (en) * | 1996-05-09 | 1998-02-10 | Meridian Diagnostics, Inc. | Immunoassay for H. pylori in fecal specimens |
CA2224551A1 (en) * | 1998-02-25 | 1999-08-25 | Alex Hongsheng Chang | A quick in situ diagnostic method for detecting alimentary diseases |
NZ528199A (en) | 1998-08-11 | 2005-06-24 | Idec Pharma Corp | Combination therapies for B-cell lyphomas comprising administration of anti-CD20 antibody |
EP1125130B1 (en) * | 1998-10-29 | 2006-07-26 | DakoCytomation Denmark A/S | Detection of acid-resistant micro-organisms in a stool |
US8557244B1 (en) | 1999-08-11 | 2013-10-15 | Biogen Idec Inc. | Treatment of aggressive non-Hodgkins lymphoma with anti-CD20 antibody |
ES2194782T5 (en) | 1999-10-12 | 2014-08-20 | Oxoid (Ely) Limited | Improved procedure for the detection of acid-resistant bacteria of the genus Helicobacter in feces |
DE60045870D1 (en) | 1999-10-29 | 2011-06-01 | Wakamoto Pharma Co Ltd | MONOCLONAL ANTIBODY, HYBRIDOMA, IMMUNE TEST METHOD AND DIAGNOSIS KIT |
JP3504633B2 (en) * | 1999-10-29 | 2004-03-08 | わかもと製薬株式会社 | Test method and test reagent for determining infection to Helicobacter pylori |
AU1939501A (en) * | 1999-12-03 | 2001-06-12 | Meridian Bioscience, Inc. | Diagnosis and treatment for helicobacter pylori induced colic |
TWI237695B (en) * | 1999-12-14 | 2005-08-11 | Joy Biomedical Corp | Helicobacter pylori antigens in blood |
DE10006432A1 (en) * | 2000-02-14 | 2001-08-16 | Ganzimmun Inst Fuer Ganzheitli | Method for the detection of Helicobacter pylori in stool and saliva samples |
DE60138981D1 (en) * | 2000-05-18 | 2009-07-30 | Meridian Bioscience Inc | Immunoassay for H. pylori in feces samples using genus-specific antibodies |
WO2002014541A1 (en) * | 2000-08-11 | 2002-02-21 | Wakamoto Pharmaceutical Co., Ltd. | Method of examining infection with helicobacter pylori and diagnostic kit |
WO2002088737A1 (en) * | 2001-04-23 | 2002-11-07 | Wakamoto Pharmaceutical Co., Ltd. | Immunochromatographic test piece and diagnosis kit |
JP4763149B2 (en) * | 2001-05-10 | 2011-08-31 | わかもと製薬株式会社 | Test method to determine infection with Helicobacter pylori |
FI118061B (en) | 2001-09-24 | 2007-06-15 | Beanor Oy | Procedure and bio donor for analysis |
FI115166B (en) | 2001-12-31 | 2005-03-15 | Biofons Oy | Diagnostic procedures |
DE10219741A1 (en) * | 2002-05-02 | 2003-11-13 | Georg S Wengler | Process for the pretreatment of stool samples |
KR20040046427A (en) * | 2002-11-27 | 2004-06-05 | 주식회사 비엘에스 | Method for detecting Helicobacter pylori in a stool sample |
WO2009005536A2 (en) * | 2006-11-22 | 2009-01-08 | 3M Innovative Properties Company | Methods of capturing bacterial whole cells and methods of analyzing samples for bacteria |
US8025880B2 (en) * | 2007-03-01 | 2011-09-27 | Helicure Ab | Immunoglobulin against Helicobacter pylori |
US20090263905A1 (en) * | 2008-04-18 | 2009-10-22 | Kim Scheuringer | Detection test assembly for detecting the presence of a substance in a sample |
US9110053B2 (en) * | 2010-08-20 | 2015-08-18 | Agilent Technologies, Inc. | Dried blood spotting paper device and method |
US8541180B1 (en) | 2010-10-11 | 2013-09-24 | Genova Diagnostics, Inc. | Compositions and methods for assessing gastrointestinal health |
CN102565391A (en) * | 2011-12-23 | 2012-07-11 | 王滔 | Immunoassay method for helicobacter pylori in gastric mucosa sample |
CN103293305A (en) * | 2012-03-05 | 2013-09-11 | 美利泰格诊断试剂(嘉兴)有限公司 | Detecting method of oral cavity helicobacter infection and saliva test board for detection |
CN102980884A (en) * | 2012-11-29 | 2013-03-20 | 江苏创生生物技术有限公司 | Chemiluminescence immunoassay for helicobacter pylori in gastric mucosa sample |
CN103149067A (en) * | 2013-02-28 | 2013-06-12 | 苏州和锐医药科技有限公司 | Method and reagent for extracting fecal protein |
KR101619862B1 (en) * | 2014-09-30 | 2016-05-12 | 주식회사 녹십자 | Kit and Method of Measuring Titers of Protein Comprising Human Fc Using Indirect Enzyme-linked Immunosorbent Assay |
DE102015000626A1 (en) * | 2015-01-22 | 2016-07-28 | Kibion Gmbh | Method for the detection of Helicobacter pylori |
CN109342722A (en) * | 2018-09-30 | 2019-02-15 | 深圳市鸿美诊断技术有限公司 | It is a kind of for quantitative determining the preparation method of Heliobacter pylori antigen reagent in excrement |
Family Cites Families (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FI76888C (en) * | 1981-04-29 | 1988-12-12 | Ciba Geigy Ag | New agents and packaging for immunological analysis. |
JP2607496B2 (en) * | 1986-01-22 | 1997-05-07 | アモコ・コーポレーション | Campylobacter detection probe |
US4942126A (en) * | 1986-08-13 | 1990-07-17 | Allegheny-Singer Research Institute | Method of extracting antigens of bacteria |
EP0281251A3 (en) * | 1987-02-04 | 1988-09-28 | International Immunoassay Laboratories, Inc. | A method for preparing a fecal sample composition for immunoassay testing |
US4923801A (en) * | 1987-04-13 | 1990-05-08 | The University Of Virginia Alumni Patents Foundation | Compositions and methods for the enrichment and isolation of Campylobacter pylori and related organisms from biological specimens and the environment |
US5459041A (en) * | 1988-02-18 | 1995-10-17 | Enteric Research Laboratories, Inc. | Campylobacter pylori antigens and uses thereof for detection of Campylobacter pylori infection |
US4882271A (en) * | 1988-03-10 | 1989-11-21 | Baylor College Of Medicine | Process for preparation of high molecular weight cell-associated protein of campylobacter pylori and use for serological detection of campylobacter pylori infection |
US5017342A (en) * | 1988-06-01 | 1991-05-21 | Ortho Diagnostic Systems Inc. | Device for immunoassay determinations |
US5571674A (en) * | 1989-09-18 | 1996-11-05 | The Trustees Of Columbia University In The City Of New York | DNA oligomers for use in detection of campylobacter pylori and methods of using such DNA oligomers |
AU644121B2 (en) * | 1989-12-04 | 1993-12-02 | Auspharm International Limited | Rapid in vitro test for helicobacter pylori using saliva |
JP2988635B2 (en) * | 1990-09-18 | 1999-12-13 | 塩野義製薬株式会社 | Monoclonal antibody against human IgE |
US5262156A (en) * | 1991-08-12 | 1993-11-16 | Hycor Biomedical, Inc. | Antigenic compositions and their use for the detection of Helicobacter pylori |
US5420014A (en) * | 1992-04-30 | 1995-05-30 | Auspharm International Ltd. | Rapid in vitro test for helicobacter pylori using saliva |
AU687002B2 (en) * | 1992-04-29 | 1998-02-19 | Auspharm International Limited | In vitro test for helicobacter pylori |
US5403924A (en) * | 1992-10-13 | 1995-04-04 | Vanderbilt University | Taga gene and methods for detecting predisposition to peptic ulceration |
WO1994010571A1 (en) * | 1992-10-30 | 1994-05-11 | T Cell Sciences, Inc. | Measurement of total molecule in a sample and methods based thereon |
GB9313437D0 (en) † | 1993-06-30 | 1993-08-11 | Smith Andrew W | Pathogenicity sequences in helicobacter pylori |
US5486361A (en) * | 1993-10-25 | 1996-01-23 | The United States Of America As Represented By The Department Of Health And Human Services | Hybridomas and monoclonal antibodies that speifically bind to GPIB on platelets and inhibit the binding of thrombin to platelets |
US5498528A (en) * | 1994-06-10 | 1996-03-12 | King; Wing | Detection of helicobacter pylori |
US5610060A (en) * | 1994-06-24 | 1997-03-11 | The United States Of America As Represented By The Department Of Health And Human Services | Isolated Helicobacter hepaticus |
US5716791A (en) * | 1996-05-09 | 1998-02-10 | Meridian Diagnostics, Inc. | Immunoassay for H. pylori in fecal specimens |
-
1996
- 1996-05-09 US US08/647,115 patent/US5716791A/en not_active Ceased
-
1997
- 1997-02-24 CA CA002198336A patent/CA2198336C/en not_active Expired - Lifetime
- 1997-02-27 AR ARP970100781A patent/AR006016A1/en active IP Right Grant
- 1997-02-28 AU AU15029/97A patent/AU698513B2/en not_active Expired
- 1997-03-12 MX MX9701877A patent/MX9701877A/en unknown
- 1997-03-12 CN CN97103112.6A patent/CN1271408C/en not_active Expired - Lifetime
- 1997-03-17 JP JP9062637A patent/JP3043999B2/en not_active Expired - Fee Related
- 1997-05-08 DE DE69709434T patent/DE69709434T3/en not_active Expired - Lifetime
- 1997-05-08 DE DE0806667T patent/DE806667T1/en active Pending
- 1997-05-08 AT AT97303147T patent/ATE211552T1/en not_active IP Right Cessation
- 1997-05-08 EP EP97303147A patent/EP0806667B2/en not_active Expired - Lifetime
- 1997-05-08 ES ES97303147T patent/ES2132044T5/en not_active Expired - Lifetime
- 1997-07-21 US US08/897,732 patent/US5871942A/en not_active Expired - Lifetime
-
1999
- 1999-01-01 GR GR990300022T patent/GR990300022T1/en unknown
-
2000
- 2000-02-08 US US09/500,365 patent/USRE38088E1/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
MX9701877A (en) | 1998-04-30 |
CN1271408C (en) | 2006-08-23 |
ES2132044T1 (en) | 1999-08-16 |
GR990300022T1 (en) | 1999-07-30 |
US5871942A (en) | 1999-02-16 |
US5716791A (en) | 1998-02-10 |
EP0806667B1 (en) | 2002-01-02 |
DE69709434T3 (en) | 2009-01-02 |
AR006016A1 (en) | 1999-07-21 |
DE69709434D1 (en) | 2002-02-07 |
ES2132044T5 (en) | 2008-12-01 |
USRE38088E1 (en) | 2003-04-22 |
AU698513B2 (en) | 1998-10-29 |
CN1165299A (en) | 1997-11-19 |
DE69709434T2 (en) | 2002-09-19 |
DE806667T1 (en) | 1999-10-07 |
ES2132044T3 (en) | 2002-03-16 |
ATE211552T1 (en) | 2002-01-15 |
EP0806667A1 (en) | 1997-11-12 |
CA2198336C (en) | 2002-06-18 |
JPH1010128A (en) | 1998-01-16 |
AU1502997A (en) | 1997-11-13 |
EP0806667B2 (en) | 2008-07-09 |
JP3043999B2 (en) | 2000-05-22 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKEX | Expiry |
Effective date: 20170224 |