CA2198899A1 - Methods for producing antibody libraries using universal or randomized immunoglobulin light chains - Google Patents
Methods for producing antibody libraries using universal or randomized immunoglobulin light chainsInfo
- Publication number
- CA2198899A1 CA2198899A1 CA002198899A CA2198899A CA2198899A1 CA 2198899 A1 CA2198899 A1 CA 2198899A1 CA 002198899 A CA002198899 A CA 002198899A CA 2198899 A CA2198899 A CA 2198899A CA 2198899 A1 CA2198899 A1 CA 2198899A1
- Authority
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- Prior art keywords
- oligonucleotide
- immunoglobulin
- light chain
- gene
- seq
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract 23
- 108010065825 Immunoglobulin Light Chains Proteins 0.000 title claims 10
- 102000013463 Immunoglobulin Light Chains Human genes 0.000 title 1
- 108091034117 Oligonucleotide Proteins 0.000 claims abstract 34
- 230000001939 inductive effect Effects 0.000 claims abstract 5
- 238000002703 mutagenesis Methods 0.000 claims abstract 5
- 231100000350 mutagenesis Toxicity 0.000 claims abstract 5
- 238000004519 manufacturing process Methods 0.000 claims abstract 4
- 239000002773 nucleotide Substances 0.000 claims 28
- 125000003729 nucleotide group Chemical group 0.000 claims 28
- 108090000623 proteins and genes Proteins 0.000 claims 19
- 108010047041 Complementarity Determining Regions Proteins 0.000 claims 16
- 230000000295 complement effect Effects 0.000 claims 14
- 108700005091 Immunoglobulin Genes Proteins 0.000 claims 10
- 108060003951 Immunoglobulin Proteins 0.000 claims 9
- 102000018358 immunoglobulin Human genes 0.000 claims 9
- 238000003752 polymerase chain reaction Methods 0.000 claims 6
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 4
- 229920001184 polypeptide Polymers 0.000 claims 4
- 108090000765 processed proteins & peptides Proteins 0.000 claims 4
- 102000004196 processed proteins & peptides Human genes 0.000 claims 4
- 108700029228 Immunoglobulin Heavy Chain Genes Proteins 0.000 claims 3
- 239000000427 antigen Substances 0.000 claims 3
- 108091007433 antigens Proteins 0.000 claims 3
- 102000036639 antigens Human genes 0.000 claims 3
- 241000894007 species Species 0.000 claims 3
- 108700029227 Immunoglobulin Light Chain Genes Proteins 0.000 claims 2
- 230000004186 co-expression Effects 0.000 claims 1
- 241000724791 Filamentous phage Species 0.000 abstract 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 1
- 239000002245 particle Substances 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/06—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations
- A61K49/08—Nuclear magnetic resonance [NMR] contrast preparations; Magnetic resonance imaging [MRI] contrast preparations characterised by the carrier
- A61K49/10—Organic compounds
- A61K49/14—Peptides, e.g. proteins
- A61K49/16—Antibodies; Immunoglobulins; Fragments thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
- A61K51/02—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by the carrier, i.e. characterised by the agent or material covalently linked or complexing the radioactive nucleus
- A61K51/04—Organic compounds
- A61K51/08—Peptides, e.g. proteins, carriers being peptides, polyamino acids, proteins
- A61K51/10—Antibodies or immunoglobulins; Fragments thereof, the carrier being an antibody, an immunoglobulin or a fragment thereof, e.g. a camelised human single domain antibody or the Fc fragment of an antibody
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
- C07K16/10—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
- C07K16/1036—Retroviridae, e.g. leukemia viruses
- C07K16/1045—Lentiviridae, e.g. HIV, FIV, SIV
- C07K16/1063—Lentiviridae, e.g. HIV, FIV, SIV env, e.g. gp41, gp110/120, gp160, V3, PND, CD4 binding site
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/44—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/55—Fab or Fab'
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
Abstract
The present invention describes methods for producing antibody libraries, and particularly for increasing antibody library diversity by inducing mutagenesis within the CDR regions of immunoglobulun heavy or light chains that are displayed on the surface of filamentous phage particles comprising the library. The invention also describes oligonucleotides useful for increasing the library diiversity, and universal light chains useful in the library production methods.
Claims (37)
1. An oligonucleotide useful as a primer for inducing mutagenesis in a complementarity determining region (CDR) of an immunoglobulin light chain gene, said oligonucleotide having 3' and 5' termini and comprising:
a) a nucleotide sequence at said 3' terminus capable of hybridizing to a first framework region of an immunoglobulin gene;
b) a nucleotide sequence at said 5' terminus capable of hybridizing to a second framework region of an immunoglobulin gene; and c) a nucleotide sequence between said 3' and 5' termini according to the formula:
[NNK]n, wherein N is independently any nucleotide, K is G or T, n is 3 to about 24, said 3' and 5' terminal nucleotide sequences having a length of about 6 to 50 nucleotides, or an oligonucleotide having a sequence complementary thereto.
a) a nucleotide sequence at said 3' terminus capable of hybridizing to a first framework region of an immunoglobulin gene;
b) a nucleotide sequence at said 5' terminus capable of hybridizing to a second framework region of an immunoglobulin gene; and c) a nucleotide sequence between said 3' and 5' termini according to the formula:
[NNK]n, wherein N is independently any nucleotide, K is G or T, n is 3 to about 24, said 3' and 5' terminal nucleotide sequences having a length of about 6 to 50 nucleotides, or an oligonucleotide having a sequence complementary thereto.
2. The oligonucleotide of claim 1 wherein said 5' terminus has the nucleotide sequence 5' -TATACTGTCAGCAGTAT-3' (SEQ ID NO
26) or 5' -GATTTTGCAGTGTATTACTGTCAGQGTAT-3' (SEQ ID NO 27), or an oligonucleotide having a sequence complementary thereto.
26) or 5' -GATTTTGCAGTGTATTACTGTCAGQGTAT-3' (SEQ ID NO 27), or an oligonucleotide having a sequence complementary thereto.
3. The oligonucleotide of claim 1 wherein said 3' terminus has the nucleotide sequence 5' -ACTTTCGGCGGAGGGACQAGGTGGAG-3' (SEQ ID NO 28) or 5' -ACTTTCGGCGGAGGGACC-3' (SEQ ID NO 29), or an oligonucleotide having a sequence complementary thereto.
4. The oligonucleotide of claim 1 wherein n is 4, 5, 6, 10 or 16.
5. The oligonucleotide of claim 1 wherein said immunoglobulin is human.
6. The oligonucleotide of claim 1 wherein said CDR is CDR3.
7. The oligonucleotide of claim 1 according to the formula: 5' -GATTTTGCAGTGTATTACTGT [NNK] 10TTCGGCGGAGGGACCAAGGTGGAG-3' (SEQ ID NO 12), or an oligonucleotide having a sequence complementary thereto.
8. An oligonucleotide useful as a primer for inducing mutagenesis in a complementarity determining region (CDR) of an immunoglobulin light chain gene, said oligonucleotide having 3' and 5' termini and comprising:
a) a nucleotide sequence at said 3' terminus capable of hybridizing to a first framework region of an immunoglobulin gene;
b) a nucleotide sequence at said 5' terminus capable of hybridizing to a second framework region of an immunoglobulin gene; and c) a nucleotide sequence between said 3' and 5' termini according to the formula:
[MNN]n, wherein N is independently any nucleotide, M is A or C, n is 3 to about 24, said 3' and 5' terminal nucleotide sequences having a length of about 6 to 50 nucleotides, or an oligonucleotide having a sequence complementary thereto.
a) a nucleotide sequence at said 3' terminus capable of hybridizing to a first framework region of an immunoglobulin gene;
b) a nucleotide sequence at said 5' terminus capable of hybridizing to a second framework region of an immunoglobulin gene; and c) a nucleotide sequence between said 3' and 5' termini according to the formula:
[MNN]n, wherein N is independently any nucleotide, M is A or C, n is 3 to about 24, said 3' and 5' terminal nucleotide sequences having a length of about 6 to 50 nucleotides, or an oligonucleotide having a sequence complementary thereto.
9 . The oligonucleotide of claim 8 wherein said 5' terminus has the nucleotide sequence 5' -GTTCCACCTTGGTCCCTTGGCCGAA-3' (SEQ
ID NO 30), or an oligonucleotide having a sequence complementary thereto.
ID NO 30), or an oligonucleotide having a sequence complementary thereto.
10. The oligonucleotide of claim 8 wherein said 3' terminus has the nucleotide sequence 5' -ACAGTAGTACACTGCAAAATC-3' (SEQ ID
NO 31), or an oligonucleotide having a sequence complementary thereto.
NO 31), or an oligonucleotide having a sequence complementary thereto.
11. The oligonucleotide of claim 8 wherein n is 8, 10 or 16.
12. The oligonucleotide of claim 8 wherein said immunoglobulin is human.
13. The oligonucleotide of claim 8 wherein said CDR is CDR3.
14. A method for producing an antibody combining site in a polypeptide comprising inducing mutagenesis in a complementarity determining region (CDR) of an immunoglobulin light chain gene which comprises amplifying a CDR portion of the immunoglobulin gene by polymerase chain reaction (PCR) using a PCR primer oligonucleotide, said oligonucleotide having 3' and 5' termini and comprising:
a) a nucleotide sequence at said 3' terminus capable of hybridizing to a first framework region of an immunoglobulin gene;
b) a nucleotide sequence at said 5' terminus capable of hybridizing to a second framework region of an immunoglobulin gene; and c) a nucleotide sequence between said 3' and 5' termini according to the formula:
[NNK]n, wherein N is independently any nucleotide, K is G or T, and n is 3 to about 24, said 3' and 5' terminal nucleotide sequences having a length of about 6 to 50 nucleotides, or an oligonucleotide having a sequence complementary thereto.
a) a nucleotide sequence at said 3' terminus capable of hybridizing to a first framework region of an immunoglobulin gene;
b) a nucleotide sequence at said 5' terminus capable of hybridizing to a second framework region of an immunoglobulin gene; and c) a nucleotide sequence between said 3' and 5' termini according to the formula:
[NNK]n, wherein N is independently any nucleotide, K is G or T, and n is 3 to about 24, said 3' and 5' terminal nucleotide sequences having a length of about 6 to 50 nucleotides, or an oligonucleotide having a sequence complementary thereto.
15. The method of claim 14 wherein said 5' terminus has the nucleotide sequence 5'-TATACTGTCAGCAGTAT-3' (SEQ ID NO 26) or 5'-GATTTTGCAGTGTATTACTGTCAGCAGTAT-3' (SEQ ID NO 27), or an oligonucleotide having a sequence complementary thereto.
16 . The method of claim 14 wherein said 3' terminus has the nucleotide sequence 5' -ACTTTCGGCGGAGGGACCAAGGTGGAG-3' (SEQ ID NO
28) or 5' -ACTTTCGGCGGAGGGACC-3' (SEQ ID NO 29), or an oligonucleotide having a sequence complementary thereto.
28) or 5' -ACTTTCGGCGGAGGGACC-3' (SEQ ID NO 29), or an oligonucleotide having a sequence complementary thereto.
17. The method of claim 14 wherein n is 4, 5, 6, 10 or 16.
18. The method of claim 14 wherein said immunoglobulin is human.
19. The method of claim 14 wherein said CDR is CDR3.
20. The method of claim 14 according to the formula:
5'-GATTTTGCAGTGTATTACTGT [NNK]10TTCGGCGGAGGGCCAAGGTGGAG-3' (SEQ ID NO
12), or an oligonucleotide having a sequence complementary thereto.
5'-GATTTTGCAGTGTATTACTGT [NNK]10TTCGGCGGAGGGCCAAGGTGGAG-3' (SEQ ID NO
12), or an oligonucleotide having a sequence complementary thereto.
21. The method of claim 14 wherein said immunoglobulin light chain gene includes a sequence having the sequence characteristics of the light chain shown in SEQ ID NO 2 or in SEQ
ID NO 62.
ID NO 62.
22. The method of claim 14 wherein said immunoglobulin light chain gene has the sequence characteristics of the light chain gene in ATCC Accession No. 75408.
23. The method of claim 14 that further comprises the steps of:
a) isolating the amplified CDR to form a library of mutagenized immunoglobulin light chain genes;
b) expressing the isolated library of mutagenized light chain genes in combination with one or more heavy chain genes to form a combinatorial antibody library of expressed heavy and light chain genes; and c) selecting species of said combinatorial antibody library for the ability to bind a preselected antigen.
a) isolating the amplified CDR to form a library of mutagenized immunoglobulin light chain genes;
b) expressing the isolated library of mutagenized light chain genes in combination with one or more heavy chain genes to form a combinatorial antibody library of expressed heavy and light chain genes; and c) selecting species of said combinatorial antibody library for the ability to bind a preselected antigen.
24. The method of claim 23 wherein said one or more immunoglobulin heavy chain genes is a library of heavy chain genes.
25. A method for producing an antibody combining site in a polypeptide comprising inducing mutagenesis in a complementarity determining region (CDR) of an immunoglobulin light chain gene which comprises amplifying a CDR portion of the immunoglobulin gene by polymerase chain reaction (PCR) using a PCR primer oligonucleotide, said oligonucleotide having 3' and 5' termini and comprising:
a) a nucleotide sequence at said 3' terminus capable of hybridizing to a first framework region of an immunoglobulin gene;
b) a nucleotide sequence at said 5' terminus capable of hybridizing to a second framework region of an immunoglobulin gene; and c) a nucleotide sequence between said 3' and 5' termini according to the formula:
[MNN]n, wherein N is independently any nucleotide, M is A or C, n is 3 to about 24, said 3' and 5' terminal nucleotide sequences having a length of about 6 to 50 nucleotides, or an oligonucleotide having a sequence complementary thereto.
a) a nucleotide sequence at said 3' terminus capable of hybridizing to a first framework region of an immunoglobulin gene;
b) a nucleotide sequence at said 5' terminus capable of hybridizing to a second framework region of an immunoglobulin gene; and c) a nucleotide sequence between said 3' and 5' termini according to the formula:
[MNN]n, wherein N is independently any nucleotide, M is A or C, n is 3 to about 24, said 3' and 5' terminal nucleotide sequences having a length of about 6 to 50 nucleotides, or an oligonucleotide having a sequence complementary thereto.
26. The method of claim 25 wherein said 5' terminus has the nucleotide sequence 5'-GTTCCACCTTGGTCCCTTGGCCGAA-3' (SEQ ID NO
30), or an oligonucleotide having a sequence complementary thereto.
30), or an oligonucleotide having a sequence complementary thereto.
27. The method of claim 25 wherein said 3' terminus has the nucleotide sequence 5'-ACAGTAGTACACTGCAAAATC-3' (SEQ ID NO 31), or an oligonucleotide having a sequence complementary thereto.
28. The method of claim 25 wherein n is 8, 10 or 16.
29. The method of claim 25 wherein said immunoglobulin is human.
30. The method of claim 25 wherein said CDR is CDR3.
31. The method of claim 25 wherein said immunoglobulin light chain gene includes a sequence having the sequence characteristics of the light chain shown in SEQ ID NO 2 or in SEQ
ID NO 62.
ID NO 62.
32. The method of claim 25 wherein said immunoglobulin light chain gene has the sequence characteristics of the light chain gene in ATCC Accession No. 75408.
33. The method of claim 25 that further comprises the steps of:
a) isolating the amplified CDR to form a library of mutagenized immunoglobulin light chain genes;
b) expressing the isolated library of mutagenized light chain genes in combination with one or more heavy chain genes to form a combinatorial antibody library of expressed heavy and light chain genes; and c) selecting species of said combinatorial antibody library for the ability to bind a preselected antigen.
a) isolating the amplified CDR to form a library of mutagenized immunoglobulin light chain genes;
b) expressing the isolated library of mutagenized light chain genes in combination with one or more heavy chain genes to form a combinatorial antibody library of expressed heavy and light chain genes; and c) selecting species of said combinatorial antibody library for the ability to bind a preselected antigen.
34. The method of claim 33 wherein said one or more immunoglobulin heavy chain genes is a library of heavy chain genes.
35. A method for producing a heterodimeric immunoglobulin molecule having immunoglobulin variable domain heavy and light chain polypeptides comprising the steps of:
a) combining an immunoglobulin variable domain light chain gene that includes a sequence having the sequence characteristics of the light chain shown in SEQ ID NO 2 or 62 with one or more immunoglobulin variable domain heavy chain genes to form a combinatorial immunoglobulin heavy and light chain gene library, said combining comprising operatively linking said light chain gene with one of said heavy chain genes in a vector capable of co-expression of said heavy and light chain genes;
b) expressing the combinatorial gene library to form a combinatorial antibody library of expressed heavy and light chain polypeptides; and c) selecting species of said combinatorial antibody library for the ability to bind a preselected antigen.
a) combining an immunoglobulin variable domain light chain gene that includes a sequence having the sequence characteristics of the light chain shown in SEQ ID NO 2 or 62 with one or more immunoglobulin variable domain heavy chain genes to form a combinatorial immunoglobulin heavy and light chain gene library, said combining comprising operatively linking said light chain gene with one of said heavy chain genes in a vector capable of co-expression of said heavy and light chain genes;
b) expressing the combinatorial gene library to form a combinatorial antibody library of expressed heavy and light chain polypeptides; and c) selecting species of said combinatorial antibody library for the ability to bind a preselected antigen.
36. The method of claim 35 wherein said immunoglobulin light chain gene has the sequence characteristics of the light chain gene in ATCC Accession No. 75408.
37. The method of claim 35 wherein said one or more immunoglobulin heavy chain genes is a library of heavy chain genes.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/300,386 US5667988A (en) | 1992-01-27 | 1994-09-02 | Methods for producing antibody libraries using universal or randomized immunoglobulin light chains |
US08/300,386 | 1994-09-02 | ||
PCT/US1995/011235 WO1996007754A1 (en) | 1994-09-02 | 1995-09-01 | Methods for producing antibody libraries using universal or randomized immunoglobulin light chains |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2198899A1 true CA2198899A1 (en) | 1996-03-14 |
CA2198899C CA2198899C (en) | 2010-02-23 |
Family
ID=23158889
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2198899A Expired - Lifetime CA2198899C (en) | 1994-09-02 | 1995-09-01 | Methods for producing antibody libraries using universal or randomized immunoglobulin light chains |
Country Status (11)
Country | Link |
---|---|
US (2) | US5667988A (en) |
EP (1) | EP0779933B1 (en) |
JP (1) | JPH10504970A (en) |
AT (1) | ATE236992T1 (en) |
AU (1) | AU706343B2 (en) |
CA (1) | CA2198899C (en) |
DE (1) | DE69530305T2 (en) |
DK (1) | DK0779933T3 (en) |
ES (1) | ES2196077T3 (en) |
PT (1) | PT779933E (en) |
WO (1) | WO1996007754A1 (en) |
Families Citing this family (356)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU6235294A (en) * | 1993-02-02 | 1994-08-29 | Scripps Research Institute, The | Methods for producing polypeptide binding sites |
US20060078561A1 (en) * | 1994-01-31 | 2006-04-13 | The Trustees Of Boston University | Polyclonal antibody libraries |
DK0744958T3 (en) | 1994-01-31 | 2003-10-20 | Univ Boston | Polyclonal antibody libraries |
DK1143006T3 (en) * | 1995-08-18 | 2008-07-14 | Morphosys Ip Gmbh | Vectors / DNA sequences from human combinatorial antibody libraries |
US6017754A (en) * | 1995-08-24 | 2000-01-25 | Invitrogen Corporation | System for isolating and identifying eukaryotic cells transfected with genes and vectors, host cells and methods thereof |
FR2741892B1 (en) | 1995-12-04 | 1998-02-13 | Pasteur Merieux Serums Vacc | METHOD FOR PREPARING A MULTI-COMBINED BANK OF ANTIBODY GENE EXPRESSION VECTORS, BANK AND COLICLONAL ANTIBODY EXPRESSION SYSTEMS |
US6696251B1 (en) | 1996-05-31 | 2004-02-24 | Board Of Trustees Of The University Of Illinois | Yeast cell surface display of proteins and uses thereof |
GB9701425D0 (en) | 1997-01-24 | 1997-03-12 | Bioinvent Int Ab | A method for in vitro molecular evolution of protein function |
US6590079B2 (en) * | 1997-01-30 | 2003-07-08 | Ixsys, Incorporated | Anti-αvβ3 recombinant human antibodies, nucleic acids encoding same |
US6596850B1 (en) * | 1998-01-30 | 2003-07-22 | Ixsys, Incorporated | Anti-αv3β3 recombinant human antibodies, nucleic acids encoding same |
US6057098A (en) * | 1997-04-04 | 2000-05-02 | Biosite Diagnostics, Inc. | Polyvalent display libraries |
EP0985033A4 (en) * | 1997-04-04 | 2005-07-13 | Biosite Inc | Polyvalent and polyclonal libraries |
GB9722131D0 (en) * | 1997-10-20 | 1997-12-17 | Medical Res Council | Method |
US6759243B2 (en) * | 1998-01-20 | 2004-07-06 | Board Of Trustees Of The University Of Illinois | High affinity TCR proteins and methods |
EP2311490A3 (en) | 1998-07-13 | 2011-05-04 | Board of Regents, The University of Texas System | Uses of antibodies to aminophospholipids for cancer treatment |
IL140918A0 (en) | 1998-07-27 | 2002-02-10 | Genentech Inc | Improved transformation efficiency in phage display through modification of a coat protein |
US6531580B1 (en) * | 1999-06-24 | 2003-03-11 | Ixsys, Inc. | Anti-αvβ3 recombinant human antibodies and nucleic acids encoding same |
US20070111259A1 (en) * | 1999-10-02 | 2007-05-17 | Medarex, Inc. | Human antibodies |
US6794132B2 (en) | 1999-10-02 | 2004-09-21 | Biosite, Inc. | Human antibodies |
US7135287B1 (en) | 1999-10-02 | 2006-11-14 | Biosite, Inc. | Human antibodies |
US6849425B1 (en) | 1999-10-14 | 2005-02-01 | Ixsys, Inc. | Methods of optimizing antibody variable region binding affinity |
US6680209B1 (en) * | 1999-12-06 | 2004-01-20 | Biosite, Incorporated | Human antibodies as diagnostic reagents |
JP2003516755A (en) * | 1999-12-15 | 2003-05-20 | ジェネンテック・インコーポレーテッド | Shotgun scanning, a combined method for mapping functional protein epitopes |
US7229619B1 (en) | 2000-11-28 | 2007-06-12 | Medimmune, Inc. | Methods of administering/dosing anti-RSV antibodies for prophylaxis and treatment |
AU2001240020B9 (en) * | 2000-03-01 | 2008-12-04 | Medimmune, Llc | High potency recombinant antibodies and method for producing them |
DK1265928T3 (en) * | 2000-01-27 | 2010-11-15 | Medimmune Llc | RSV neutralizing antibodies with ultra high affinity |
UA81743C2 (en) | 2000-08-07 | 2008-02-11 | Центокор, Инк. | HUMAN MONOCLONAL ANTIBODY WHICH SPECIFICALLY BINDS TUMOR NECROSIS FACTOR ALFA (TNFα), PHARMACEUTICAL MIXTURE CONTAINING THEREOF, AND METHOD FOR TREATING ARTHRITIS |
US7288390B2 (en) | 2000-08-07 | 2007-10-30 | Centocor, Inc. | Anti-dual integrin antibodies, compositions, methods and uses |
US6902734B2 (en) | 2000-08-07 | 2005-06-07 | Centocor, Inc. | Anti-IL-12 antibodies and compositions thereof |
US7198924B2 (en) | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
US6818216B2 (en) | 2000-11-28 | 2004-11-16 | Medimmune, Inc. | Anti-RSV antibodies |
US6855493B2 (en) | 2000-11-28 | 2005-02-15 | Medimmune, Inc. | Methods of administering/dosing anti-RSV antibodies for prophylaxis and treatment |
US7179900B2 (en) * | 2000-11-28 | 2007-02-20 | Medimmune, Inc. | Methods of administering/dosing anti-RSV antibodies for prophylaxis and treatment |
US7087726B2 (en) | 2001-02-22 | 2006-08-08 | Genentech, Inc. | Anti-interferon-α antibodies |
CA2450236A1 (en) * | 2001-07-06 | 2003-01-16 | Genentech, Inc. | Phage displayed pdz domain ligands |
US20030104604A1 (en) * | 2001-08-03 | 2003-06-05 | Weiping Yang | Genetically engineered bacterial strains for the display of foreign peptides on filamentous phage |
DE60232688D1 (en) | 2001-11-20 | 2009-07-30 | Univ Duke | BOUNDARY-BIOMATERIALS |
GB0202206D0 (en) * | 2002-01-31 | 2002-03-20 | Bioinvent Int Ab | Method of making libraries of anti-ligands |
US7504364B2 (en) * | 2002-03-01 | 2009-03-17 | Receptors Llc | Methods of making arrays and artificial receptors |
US7244592B2 (en) | 2002-03-07 | 2007-07-17 | Dyax Corp. | Ligand screening and discovery |
DE60328674D1 (en) * | 2002-04-17 | 2009-09-17 | Bioren Inc | UNIVERSAL BANKS FOR IMMUNOGLOBULINE |
US7405062B2 (en) * | 2002-05-14 | 2008-07-29 | San Diego Antibody | Method for cloning variable domain sequences of immunological gene repertoire |
EP1513879B1 (en) * | 2002-06-03 | 2018-08-22 | Genentech, Inc. | Synthetic antibody phage libraries |
US7132100B2 (en) | 2002-06-14 | 2006-11-07 | Medimmune, Inc. | Stabilized liquid anti-RSV antibody formulations |
US7425618B2 (en) * | 2002-06-14 | 2008-09-16 | Medimmune, Inc. | Stabilized anti-respiratory syncytial virus (RSV) antibody formulations |
US20040175378A1 (en) | 2002-07-15 | 2004-09-09 | Board Of Regents, The University Of Texas System | Selected antibody compositions and methods for binding to aminophospholipids |
PT2314629E (en) * | 2002-07-18 | 2014-01-22 | Merus B V | Recombinant production of mixtures of antibodies |
USRE47770E1 (en) | 2002-07-18 | 2019-12-17 | Merus N.V. | Recombinant production of mixtures of antibodies |
TW200501985A (en) * | 2002-07-25 | 2005-01-16 | Medimmune Inc | Methods of treating and preventing RSV, hMPV, and PIV using anti-RSV, anti-hMPV, and anti-PIV antibodies |
US20040067532A1 (en) | 2002-08-12 | 2004-04-08 | Genetastix Corporation | High throughput generation and affinity maturation of humanized antibody |
US7469076B2 (en) * | 2003-09-03 | 2008-12-23 | Receptors Llc | Sensors employing combinatorial artificial receptors |
WO2005003326A2 (en) * | 2003-03-28 | 2005-01-13 | Receptors Llc. | Artificial receptors including reversibly immobilized building blocks and methods |
US20060057625A1 (en) * | 2002-09-16 | 2006-03-16 | Carlson Robert E | Scaffold-based artificial receptors and methods |
US20050037428A1 (en) * | 2002-09-16 | 2005-02-17 | Receptors Llc | Artificial receptors including reversibly immobilized building blocks, the building blocks, and methods |
US20040137481A1 (en) * | 2002-09-16 | 2004-07-15 | Receptors Llc | Artificial receptor building blocks, components, and kits |
US20050037429A1 (en) * | 2003-03-28 | 2005-02-17 | Receptors Llc | Artificial receptors including reversibly immobilized building blocks and methods |
US20050170385A1 (en) * | 2002-09-16 | 2005-08-04 | Receptors Llc | Artificial receptors including gradients |
US20050037381A1 (en) * | 2002-09-16 | 2005-02-17 | Receptors Llc | Artificial receptors, building blocks, and methods |
US20050136483A1 (en) * | 2003-09-03 | 2005-06-23 | Receptors Llc | Nanodevices employing combinatorial artificial receptors |
HUE034378T2 (en) | 2002-10-16 | 2018-02-28 | Purdue Pharma Lp | Antibodies that bind cell-associated CA 125/O722P and methods of use thereof |
US7427469B2 (en) * | 2002-11-05 | 2008-09-23 | Institut Pasteur | Method of treating cytomegalovirus with DC-SIGN blockers |
AU2003301804A1 (en) * | 2002-11-05 | 2004-06-07 | Institut National De La Sante Et De La Recherche Medicale | Dc-sign blockers and their use for preventing or treating viral infections. |
WO2004044161A2 (en) * | 2002-11-06 | 2004-05-27 | Fraunhofer Usa | Expression of foreign sequences in plants using trans-activation system |
US7692063B2 (en) * | 2002-11-12 | 2010-04-06 | Ibio, Inc. | Production of foreign nucleic acids and polypeptides in sprout systems |
US7683238B2 (en) * | 2002-11-12 | 2010-03-23 | iBio, Inc. and Fraunhofer USA, Inc. | Production of pharmaceutically active proteins in sprouted seedlings |
US20050079574A1 (en) * | 2003-01-16 | 2005-04-14 | Genentech, Inc. | Synthetic antibody phage libraries |
AU2004209660A1 (en) | 2003-02-03 | 2004-08-19 | Fraunhofer Usa, Inc. | System for expression of genes in plants |
WO2004072266A2 (en) * | 2003-02-13 | 2004-08-26 | Kalobios Inc. | Antibody affinity engineering by serial epitope-guided complementarity replacement |
DE602004025332D1 (en) | 2003-03-14 | 2010-03-18 | Wyeth Corp | ANTIBODY TO IL21 RECEPTOR AND ITS USE |
AU2004272972A1 (en) | 2003-05-22 | 2005-03-24 | Fraunhofer Usa, Inc. | Recombinant carrier molecule for expression, delivery and purification of target polypeptides |
US9708410B2 (en) | 2003-05-30 | 2017-07-18 | Janssen Biotech, Inc. | Anti-tissue factor antibodies and compositions |
US20100069614A1 (en) | 2008-06-27 | 2010-03-18 | Merus B.V. | Antibody producing non-human mammals |
ES2408582T3 (en) | 2003-05-30 | 2013-06-21 | Merus B.V. | Fab library for the preparation of a mixture of antibodies |
EP2508608A1 (en) | 2003-06-09 | 2012-10-10 | Alnylam Pharmaceuticals Inc. | Method of treating neurodegenerative disease |
WO2005011580A2 (en) * | 2003-07-25 | 2005-02-10 | Board Of Regents, The University Of Texas System | Compositions and methods for herpes simplex prophylaxis and treatment |
CA2534055A1 (en) * | 2003-08-01 | 2005-02-10 | Genentech, Inc. | Antibody cdr polypeptide sequences with restricted diversity |
WO2005035569A2 (en) * | 2003-10-10 | 2005-04-21 | Five Prime Therapeutics, Inc. | Kiaa0779, splice variants thereof, and methods of their use |
US7329725B1 (en) * | 2003-10-29 | 2008-02-12 | Nastech Pharmaceutical Company Inc. | Phage displayed Trp cage ligands |
EP1697534B1 (en) | 2003-12-01 | 2010-06-02 | Life Technologies Corporation | Nucleic acid molecules containing recombination sites and methods of using the same |
EP1737971B1 (en) | 2004-01-20 | 2017-08-16 | Merus N.V. | Mixtures of binding proteins |
EP1761561B1 (en) | 2004-01-20 | 2015-08-26 | KaloBios Pharmaceuticals, Inc. | Antibody specificity transfer using minimal essential binding determinants |
WO2005081905A2 (en) * | 2004-02-20 | 2005-09-09 | Fraunhofer Usa Inc. | Systems and methods for clonal expression in plants |
US7785903B2 (en) * | 2004-04-09 | 2010-08-31 | Genentech, Inc. | Variable domain library and uses |
US20060292554A1 (en) * | 2004-05-18 | 2006-12-28 | Genentech, Inc. | Major coat protein variants for C-terminal and bi-terminal display |
ATE462451T1 (en) | 2004-06-16 | 2010-04-15 | Affinergy Inc | INTERFACE BIOMATERIALS TO PROMOTE ADHESION OF TARGET ANALYTES |
EP1781680B8 (en) * | 2004-07-06 | 2016-05-18 | Bioren, LLC | Universal antibody libraries |
EP1791975A4 (en) * | 2004-08-05 | 2007-11-07 | Biosite Inc | Compositions and methods for phage display of polypeptides |
US7504365B2 (en) | 2004-09-03 | 2009-03-17 | Receptors Llc | Combinatorial artificial receptors including tether building blocks |
WO2006029383A2 (en) * | 2004-09-11 | 2006-03-16 | Receptors Llc | Combinatorial artificial receptors including peptide building blocks |
EP1812068A4 (en) * | 2004-10-29 | 2010-06-09 | Medimmune Inc | Methods of preventing and treating rsv infections and related conditions |
US10011858B2 (en) | 2005-03-31 | 2018-07-03 | Chugai Seiyaku Kabushiki Kaisha | Methods for producing polypeptides by regulating polypeptide association |
WO2006109592A1 (en) * | 2005-04-08 | 2006-10-19 | Chugai Seiyaku Kabushiki Kaisha | Antibody substituting for function of blood coagulation factor viii |
HUE030701T2 (en) | 2005-05-27 | 2017-05-29 | Biogen Ma Inc | Tweak binding antibodies |
EP1919504B1 (en) * | 2005-08-03 | 2013-10-16 | iBio, Inc. | Antibody to bacillus anthracis protective antigen |
IL172297A (en) | 2005-10-03 | 2016-03-31 | Compugen Ltd | Soluble vegfr-1 variants for diagnosis of preeclamsia |
WO2007048127A2 (en) * | 2005-10-20 | 2007-04-26 | The Scripps Research Institute | Fc labeling for immunostaining and immunotargeting |
ES2577292T3 (en) | 2005-11-07 | 2016-07-14 | Genentech, Inc. | Binding polypeptides with diversified VH / VL hypervariable sequences and consensus |
US8716195B2 (en) * | 2005-11-14 | 2014-05-06 | Bioren, Inc. | Antibody ultrahumanization by predicted mature CDR blasting and cohort library generation and screening |
CA2626522A1 (en) | 2005-11-16 | 2007-05-24 | Ambrx, Inc. | Methods and compositions comprising non-natural amino acids |
US20070237764A1 (en) * | 2005-12-02 | 2007-10-11 | Genentech, Inc. | Binding polypeptides with restricted diversity sequences |
EP2325208B1 (en) | 2005-12-15 | 2017-09-20 | Genentech, Inc. | Polyubiquitin antibodies |
AU2007248444B2 (en) | 2006-01-05 | 2012-10-25 | Genentech, Inc. | Anti-EphB4 antibodies and methods using same |
US20100184021A1 (en) | 2006-01-16 | 2010-07-22 | Compugen Ltd. | Novel nucleotide and amino acid sequences, and methods of use thereof for diagnosis |
WO2007092777A2 (en) * | 2006-02-02 | 2007-08-16 | Wyeth | Cloning, characterization, and application of tnfrsf19 in neurological disorders |
WO2008048344A2 (en) * | 2006-02-13 | 2008-04-24 | Fraunhofer Usa, Inc. | Bacillus anthracis antigens, vaccine compositions, and related methods |
EP1984405A4 (en) * | 2006-02-13 | 2010-06-30 | Fraunhofer Usa Inc | Influenza antigens, vaccine compositions, and related methods |
CA2642056A1 (en) * | 2006-02-13 | 2007-08-23 | Fraunhofer Usa, Inc. | Hpv antigens, vaccine compositions, and related methods |
AR059851A1 (en) | 2006-03-16 | 2008-04-30 | Genentech Inc | ANTIBODIES OF EGFL7 AND METHODS OF USE |
US7833724B2 (en) * | 2006-03-20 | 2010-11-16 | Teva Women's Health, Inc. | Methods for comparing the immunogenicity of products and uses thereof |
US11046784B2 (en) | 2006-03-31 | 2021-06-29 | Chugai Seiyaku Kabushiki Kaisha | Methods for controlling blood pharmacokinetics of antibodies |
EP3345616A1 (en) | 2006-03-31 | 2018-07-11 | Chugai Seiyaku Kabushiki Kaisha | Antibody modification method for purifying bispecific antibody |
WO2007134050A2 (en) * | 2006-05-09 | 2007-11-22 | Genentech, Inc. | Binding polypeptides with optimized scaffolds |
US8148085B2 (en) | 2006-05-15 | 2012-04-03 | Sea Lane Biotechnologies, Llc | Donor specific antibody libraries |
EP2522678A1 (en) * | 2006-05-15 | 2012-11-14 | Sea Lane Biotechnologies, LLC | Neutralizing antibodies to influenza viruses |
RU2436796C9 (en) | 2006-05-30 | 2013-12-27 | Дженентек, Инк. | Antibodies and immunoconjugates and their application |
EP2032604A2 (en) | 2006-06-06 | 2009-03-11 | Genentech, Inc. | Anti-dll4 antibodies and methods using same |
CA2657576C (en) | 2006-07-14 | 2023-10-31 | The Regents Of The University Of California | Cancer biomarkers and methods of use thereof |
US20090252745A1 (en) * | 2006-09-15 | 2009-10-08 | Duke University | Amino acids in the HCV core polypeptide domain 3 and correlation with steatosis |
DK2069558T3 (en) | 2006-10-02 | 2013-08-05 | Sea Lane Biotechnologies Llc | Design and construction of diverse synthetic peptide and polypeptide libraries |
KR101541550B1 (en) | 2006-10-27 | 2015-08-04 | 제넨테크, 인크. | Antibodies and immunoconjugates and uses therefor |
AU2008205330B2 (en) | 2007-01-08 | 2014-07-03 | Government Of The Usa, As Represented By The Secretary, Department Of Health And Human Services | SLCO1B3 genotype |
US9068003B2 (en) | 2007-01-10 | 2015-06-30 | The United States Of America, As Represented By The Secretary, Dept. Of Health And Human Services | Antibodies that bind to TL1A and methods of treating inflammatory or autoimmune disease comprising administering such antibodies |
US9896511B2 (en) | 2007-01-10 | 2018-02-20 | The United States Of America, As Represented By The Secretary, Dept. Of Health And Human Services | Antibodies that bind to TL1A and methods of treating inflammatory or autoimmune disease comprising administering such antibodies |
US7960139B2 (en) | 2007-03-23 | 2011-06-14 | Academia Sinica | Alkynyl sugar analogs for the labeling and visualization of glycoconjugates in cells |
US8778348B2 (en) * | 2007-04-28 | 2014-07-15 | Ibio Inc. | Trypanosoma antigens, vaccine compositions, and related methods |
EP1997830A1 (en) * | 2007-06-01 | 2008-12-03 | AIMM Therapeutics B.V. | RSV specific binding molecules and means for producing them |
EP2178558B1 (en) | 2007-07-11 | 2014-04-30 | iBio, Inc. | Yersinia pestis antigens, vaccine compositions, and related methods |
SG183044A1 (en) | 2007-07-16 | 2012-08-30 | Genentech Inc | Humanized anti-cd79b antibodies and immunoconjugatesand methods of use |
ES2381788T3 (en) | 2007-07-16 | 2012-05-31 | Genentech, Inc. | Anti-CD79b and immunoconjugate antibodies and methods of use |
US20110059130A1 (en) * | 2007-08-20 | 2011-03-10 | Fraunhofer Usa, Inc. | Prophylactic and therapeutic influenza vaccines, antigens, compositions and methods |
WO2009026496A1 (en) * | 2007-08-22 | 2009-02-26 | University Of Southern California | Grp78 and tumor angiogenesis |
WO2009036379A2 (en) * | 2007-09-14 | 2009-03-19 | Adimab, Inc. | Rationally designed, synthetic antibody libraries and uses therefor |
US8877688B2 (en) | 2007-09-14 | 2014-11-04 | Adimab, Llc | Rationally designed, synthetic antibody libraries and uses therefor |
US9096651B2 (en) | 2007-09-26 | 2015-08-04 | Chugai Seiyaku Kabushiki Kaisha | Method of modifying isoelectric point of antibody via amino acid substitution in CDR |
WO2009054435A1 (en) | 2007-10-24 | 2009-04-30 | Otsuka Chemical Co., Ltd. | Polypeptide having enhanced effector function |
JP5809415B2 (en) | 2007-11-09 | 2015-11-10 | ペレグリン ファーマシューティカルズ,インコーポレーテッド | Compositions and methods of anti-VEGF antibodies |
TWI580694B (en) | 2007-11-30 | 2017-05-01 | 建南德克公司 | Anti-vegf antibodies |
US8133488B2 (en) | 2008-01-18 | 2012-03-13 | Genentech, Inc. | Methods and compositions for targeting polyubiquitin |
UA106586C2 (en) | 2008-01-31 | 2014-09-25 | Дженентек, Інк. | Anti-cd79b antibodies and imunokonugate and methods for their use |
LT2708559T (en) | 2008-04-11 | 2018-06-11 | Chugai Seiyaku Kabushiki Kaisha | Antigen-binding molecule capable of binding to two or more antigen molecules repeatedly |
CA2724415C (en) * | 2008-05-15 | 2016-09-13 | Selexys Pharmaceuticals Corporation | Anti-psgl-1 antibodies and methods of identification and use |
US20090291073A1 (en) * | 2008-05-20 | 2009-11-26 | Ward Keith W | Compositions Comprising PKC-theta and Methods for Treating or Controlling Ophthalmic Disorders Using Same |
JP2009278908A (en) * | 2008-05-22 | 2009-12-03 | Fujifilm Corp | Anti-osteocalcin antibody and immunity-measuring method using the same |
EP2303318A2 (en) | 2008-06-20 | 2011-04-06 | Wyeth LLC | Compositions and methods of use of orf1358 from beta-hemolytic streptococcal strains |
US8680020B2 (en) | 2008-07-15 | 2014-03-25 | Academia Sinica | Glycan arrays on PTFE-like aluminum coated glass slides and related methods |
CN102159243B (en) * | 2008-07-21 | 2015-08-19 | 免疫医疗公司 | For the structural variant of the antibody for the treatment of feature improved |
WO2010017598A1 (en) | 2008-08-14 | 2010-02-18 | Arana Therapeutics Limited | Anti-il-12/il-23 antibodies |
WO2010019120A1 (en) | 2008-08-15 | 2010-02-18 | The Government Of The United States Of America, As Represented By The Secretary, Department Of Health Of Human Services, National Institutes Of Health | S0x9, prostaglandin d2 and retinoic acid for treating pigmentary conditions and melanoma |
WO2010033229A2 (en) | 2008-09-22 | 2010-03-25 | Calmune Corporation | Methods and vectors for display of molecules and displayed molecules and collections |
WO2010033237A2 (en) * | 2008-09-22 | 2010-03-25 | Calmune Corporation | Methods for creating diversity in libraries and libraries, display vectors and methods, and displayed molecules |
US8734803B2 (en) | 2008-09-28 | 2014-05-27 | Ibio Inc. | Humanized neuraminidase antibody and methods of use thereof |
EP3025727A1 (en) | 2008-10-02 | 2016-06-01 | The J. David Gladstone Institutes | Methods of treating liver disease |
EP2361093A2 (en) | 2008-11-26 | 2011-08-31 | Five Prime Therapeutics, Inc. | Compositions and methods for regulating collagen and smooth muscle actin expression by serpine2 |
WO2010061393A1 (en) | 2008-11-30 | 2010-06-03 | Compugen Ltd. | He4 variant nucleotide and amino acid sequences, and methods of use thereof |
CN101768213B (en) | 2008-12-30 | 2012-05-30 | 中国科学院遗传与发育生物学研究所 | Protein related to plant tillering number and coding gene and application thereof |
CN101817879A (en) | 2009-02-26 | 2010-09-01 | 中国科学院遗传与发育生物学研究所 | Metallothionein and encoding gene and application thereof |
EP2679600A1 (en) | 2009-03-25 | 2014-01-01 | Genentech, Inc. | Anti-FGFR3 antibodies and methods using same |
RU2587621C2 (en) | 2009-04-01 | 2016-06-20 | Дженентек, Инк. | ANTI-FcRH5 ANTIBODIES, IMMUNOCONJUGATES THEREOF AND METHODS FOR USE THEREOF |
WO2010129033A2 (en) * | 2009-04-29 | 2010-11-11 | Calmune Corporation | Modified antibodies for passive immunotherapy |
JP5797642B2 (en) * | 2009-05-20 | 2015-10-21 | ノビミューン エスアー | Synthetic polypeptide libraries and methods for generating naturally diversified polypeptide variants |
DK2435568T3 (en) | 2009-05-29 | 2014-09-08 | Morphosys Ag | Collection of synthetic antibodies to treat disease |
WO2011014457A1 (en) | 2009-07-27 | 2011-02-03 | Genentech, Inc. | Combination treatments |
ES2553440T3 (en) * | 2009-08-13 | 2015-12-09 | Crucell Holland B.V. | Antibodies against human respiratory syncytial virus (RSV) and method of use |
AU2010289400B2 (en) | 2009-09-02 | 2014-10-23 | Curis, Inc. | Mutant smoothened and methods of using the same |
US20110189183A1 (en) | 2009-09-18 | 2011-08-04 | Robert Anthony Williamson | Antibodies against candida, collections thereof and methods of use |
WO2011041391A1 (en) | 2009-09-29 | 2011-04-07 | Fraunhofer Usa, Inc. | Influenza hemagglutinin antibodies, compositions, and related methods |
US8568726B2 (en) | 2009-10-06 | 2013-10-29 | Medimmune Limited | RSV specific binding molecule |
CA2778481A1 (en) | 2009-10-22 | 2011-04-28 | Genentech, Inc. | Anti-hepsin antibodies and methods using same |
WO2011056494A1 (en) | 2009-10-26 | 2011-05-12 | Genentech, Inc. | Activin receptor-like kinase-1 antagonist and vegfr3 antagonist combinations |
WO2011056502A1 (en) | 2009-10-26 | 2011-05-12 | Genentech, Inc. | Bone morphogenetic protein receptor type ii compositions and methods of use |
WO2011056497A1 (en) | 2009-10-26 | 2011-05-12 | Genentech, Inc. | Activin receptor type iib compositions and methods of use |
EP2496600A1 (en) | 2009-11-04 | 2012-09-12 | Fabrus LLC | Methods for affinity maturation-based antibody optimization |
EP2496601B1 (en) | 2009-11-05 | 2017-06-07 | F. Hoffmann-La Roche AG | Methods and composition for secretion of heterologous polypeptides |
US11377485B2 (en) | 2009-12-02 | 2022-07-05 | Academia Sinica | Methods for modifying human antibodies by glycan engineering |
US10087236B2 (en) | 2009-12-02 | 2018-10-02 | Academia Sinica | Methods for modifying human antibodies by glycan engineering |
TWI505836B (en) | 2009-12-11 | 2015-11-01 | Genentech Inc | Anti-vegf-c antibodies and methods using same |
SG2014011340A (en) | 2009-12-21 | 2014-07-30 | Genentech Inc | Antibody formulation |
MX2012007379A (en) | 2009-12-23 | 2012-08-31 | Genentech Inc | Anti-bv8 antibodies and uses thereof. |
US8685524B2 (en) | 2010-01-29 | 2014-04-01 | Toray Industries, Inc. | Polylactic acid-based resin sheet |
US9796788B2 (en) | 2010-02-08 | 2017-10-24 | Regeneron Pharmaceuticals, Inc. | Mice expressing a limited immunoglobulin light chain repertoire |
US20130045492A1 (en) | 2010-02-08 | 2013-02-21 | Regeneron Pharmaceuticals, Inc. | Methods For Making Fully Human Bispecific Antibodies Using A Common Light Chain |
AU2011213585B2 (en) * | 2010-02-08 | 2014-02-06 | Regeneron Pharmaceuticals, Inc. | Common light chain mouse |
WO2011130332A1 (en) | 2010-04-12 | 2011-10-20 | Academia Sinica | Glycan arrays for high throughput screening of viruses |
EP3508854A1 (en) | 2010-04-27 | 2019-07-10 | The Regents of The University of California | Cancer biomarkers and methods of use thereof |
JP5947289B2 (en) | 2010-05-10 | 2016-07-06 | アカデミア シニカAcademia Sinica | Determination of the susceptibility of zanamivir phosphonate congeners with anti-influenza activity and influenza virus to oseltamivir |
KR20130098165A (en) | 2010-06-03 | 2013-09-04 | 제넨테크, 인크. | Immuno-pet imaging of antibodies and immunoconjugates and uses therefor |
AR082149A1 (en) | 2010-07-09 | 2012-11-14 | Calmune Corp | ANTIBODIES AGAINST HUMAN RESPIRATORY SYNTHETIC VIRUS (RSV) AND METHODS FOR USE |
WO2012019061A2 (en) | 2010-08-05 | 2012-02-09 | Stem Centrx, Inc. | Novel effectors and methods of use |
MX2013002255A (en) | 2010-08-27 | 2013-07-03 | Stem Centrx Inc | Notum protein modulators and methods of use. |
CA2810016A1 (en) | 2010-09-03 | 2012-03-08 | Stem Centrx, Inc. | Novel modulators and methods of use |
MX349622B (en) | 2010-09-08 | 2017-08-07 | Halozyme Inc | Methods for assessing and identifying or evolving conditionally active therapeutic proteins. |
WO2012047968A2 (en) | 2010-10-05 | 2012-04-12 | Genentech, Inc. | Mutant smoothened and methods of using the same |
EP2632489B1 (en) | 2010-10-27 | 2020-01-15 | The Research Foundation for The State University of New York | Compositions targeting the soluble extracellular domain of e-cadherin and related methods for cancer therapy |
HUE038305T2 (en) | 2010-11-17 | 2018-10-29 | Chugai Pharmaceutical Co Ltd | Multi-specific antigen-binding molecule having alternative function to function of blood coagulation factor viii |
ES2696548T3 (en) | 2010-11-19 | 2019-01-16 | Morphosys Ag | A collection of antibody sequences and their use |
CA2820885A1 (en) | 2010-12-08 | 2012-09-07 | Stem Centrx, Inc. | Novel modulators and methods of use |
WO2012092539A2 (en) | 2010-12-31 | 2012-07-05 | Takeda Pharmaceutical Company Limited | Antibodies to dll4 and uses thereof |
SA112330278B1 (en) | 2011-02-18 | 2015-10-09 | ستيم سينتركس، انك. | Novel modulators and methods of use |
CN103619877B (en) | 2011-04-21 | 2018-01-02 | 加文医学研究所 | The variable domains molecule and its generation and application method B of modification |
WO2013022782A1 (en) | 2011-08-05 | 2013-02-14 | Regeneron Pharmaceuticals, Inc. | Humanized universal light chain mice |
US20130058947A1 (en) | 2011-09-02 | 2013-03-07 | Stem Centrx, Inc | Novel Modulators and Methods of Use |
CN104053779B (en) | 2011-09-28 | 2017-05-24 | 时代生物技术股份公司 | Split inteins and uses thereof |
JP5936145B2 (en) * | 2011-09-29 | 2016-06-15 | Necソリューションイノベータ株式会社 | Nucleic acid construct used for screening of peptide antibody and screening method using the same |
EP2762493B1 (en) | 2011-09-30 | 2021-06-09 | Chugai Seiyaku Kabushiki Kaisha | Antigen-binding molecule promoting disappearance of antigens having plurality of biological activities |
WO2013065708A1 (en) | 2011-10-31 | 2013-05-10 | 中外製薬株式会社 | Antigen-binding molecule having regulated conjugation between heavy-chain and light-chain |
AU2013208007A1 (en) | 2012-01-09 | 2014-07-31 | The Scripps Research Institute | Humanized antibodies with ultralong CDR3 |
JP6684490B2 (en) | 2012-01-09 | 2020-04-22 | ザ・スクリップス・リサーチ・インスティテュート | Ultralong complementarity determining regions and uses thereof |
CA2865404C (en) | 2012-02-24 | 2019-08-27 | Stem Centrx, Inc. | Dll3-binding antibodies and drug conjugates thereof to treat cancer |
US20140170159A9 (en) | 2012-03-08 | 2014-06-19 | Ge Wei | Conditionally active anti-epidermal growth factor receptor antibodies and methods of use thereof |
AU2013204581B2 (en) | 2012-03-16 | 2015-06-25 | Regeneron Pharmaceuticals, Inc. | Non-human animals expressing pH-sensitive immunoglobulin sequences |
CA2865643A1 (en) | 2012-03-16 | 2013-09-19 | Regeneron Pharmaceuticals, Inc. | Histidine engineered light chain antibodies and genetically modified non-human animals for generating the same |
RU2664473C2 (en) | 2012-03-16 | 2018-08-17 | Регенерон Фармасьютикалз, Инк. | Non-human animals expressing ph-sensitive immunoglobulin sequences |
US20140087362A1 (en) | 2012-03-16 | 2014-03-27 | Aladar A. Szalay | Methods for assessing effectiveness and monitoring oncolytic virus treatment |
US20140013456A1 (en) | 2012-03-16 | 2014-01-09 | Regeneron Pharmaceuticals, Inc. | Histidine Engineered Light Chain Antibodies and Genetically Modified Non-Human Animals for Generating the Same |
US10130714B2 (en) | 2012-04-14 | 2018-11-20 | Academia Sinica | Enhanced anti-influenza agents conjugated with anti-inflammatory activity |
HUE045944T2 (en) | 2012-04-20 | 2020-02-28 | Merus Nv | Methods and means for the production of heterodimeric ig-like molecules |
DK2692865T3 (en) * | 2012-07-30 | 2015-02-16 | Nbe Therapeutics Llc | Transponeringsmedieret identification of specific binding proteins, or functional |
JP6302909B2 (en) | 2012-08-18 | 2018-03-28 | アカデミア シニカAcademia Sinica | Cell-permeable probes for sialidase identification and imaging |
AU2013305827A1 (en) | 2012-08-21 | 2015-03-05 | Academia Sinica | Benzocyclooctyne compounds and uses thereof |
PT2928923T (en) | 2012-12-10 | 2020-03-27 | Biogen Ma Inc | Anti-blood dendritic cell antigen 2 antibodies and uses thereof |
US10717965B2 (en) | 2013-01-10 | 2020-07-21 | Gloriana Therapeutics, Inc. | Mammalian cell culture-produced neublastin antibodies |
PL2958944T3 (en) | 2013-02-22 | 2019-09-30 | Abbvie Stemcentrx Llc | Antidll3-antibody-pbd conjugates and uses thereof |
US10653779B2 (en) | 2013-03-13 | 2020-05-19 | Genentech, Inc. | Formulations with reduced oxidation |
MY189047A (en) | 2013-03-13 | 2022-01-21 | Genentech Inc | Antibody formulations |
BR112015022484A2 (en) | 2013-03-13 | 2017-07-18 | Genentech Inc | reduced oxidation formulations |
RU2707550C2 (en) | 2013-03-13 | 2019-11-27 | Дженентек, Инк. | Compositions with reduced oxidation |
AR095398A1 (en) | 2013-03-13 | 2015-10-14 | Genentech Inc | FORMULATIONS WITH REDUCED OXIDATION |
CN105392801A (en) | 2013-03-15 | 2016-03-09 | 比奥根Ma公司 | Treatment and prevention of acute kidney injury using anti-alpha v beta 5 antibodies |
CA2903589A1 (en) | 2013-03-15 | 2014-09-18 | Genentech, Inc. | Cell culture media and methods of antibody production |
EP3712252A1 (en) | 2013-03-15 | 2020-09-23 | F. Hoffmann-La Roche AG | Cell culture compositions with antioxidants and methods for polypeptide production |
WO2014210397A1 (en) | 2013-06-26 | 2014-12-31 | Academia Sinica | Rm2 antigens and use thereof |
US9981030B2 (en) | 2013-06-27 | 2018-05-29 | Academia Sinica | Glycan conjugates and use thereof |
WO2015010100A2 (en) | 2013-07-18 | 2015-01-22 | Fabrus, Inc. | Humanized antibodies with ultralong complementarity determining regions |
WO2015017146A2 (en) | 2013-07-18 | 2015-02-05 | Fabrus, Inc. | Antibodies with ultralong complementarity determining regions |
DK3036320T3 (en) | 2013-08-19 | 2021-07-12 | Biogen Ma Inc | MANAGEMENT OF PROTEIN GLYCOSYLATION BY CULTIVATION MEDIA ADDITION AND CELL CULTURE PROCESS PARAMETERS |
JP2016538318A (en) | 2013-08-28 | 2016-12-08 | ステムセントリックス, インコーポレイテッド | New SEZ6 modulator and method of use |
PE20160674A1 (en) | 2013-08-28 | 2016-07-21 | Stemcentrx Inc | METHODS OF CONJUGATION OF SITE-SPECIFIC ANTIBODIES AND COMPOSITIONS |
CN105682666B (en) | 2013-09-06 | 2021-06-01 | 中央研究院 | Activation of human iNKT cells using glycolipids |
SG11201601770YA (en) | 2013-09-12 | 2016-04-28 | Halozyme Inc | Modified anti-epidermal growth factor receptor antibodies and methods of use thereof |
WO2015048330A2 (en) | 2013-09-25 | 2015-04-02 | Biogen Idec Ma Inc. | On-column viral inactivation methods |
PT3050896T (en) | 2013-09-27 | 2021-08-24 | Chugai Pharmaceutical Co Ltd | Method for producing polypeptide heteromultimer |
TWI714022B (en) | 2013-09-27 | 2020-12-21 | 美商建南德克公司 | Anti-pdl1 antibody formulations |
BR112016011046A2 (en) | 2013-11-15 | 2019-01-29 | Centre Nat Rech Scient | method for genotyping, method for detecting an infection, kit for detecting an infection and method for detecting in a biological sample. |
WO2015095809A1 (en) | 2013-12-20 | 2015-06-25 | Biogen Idec Ma Inc. | Use of perfusion seed cultures to improve biopharmaceutical fed-batch production capacity and product quality |
EP2960252A1 (en) | 2014-06-26 | 2015-12-30 | Institut Pasteur | Phospholipase for treatment of immunosuppression |
CA2935378C (en) | 2013-12-24 | 2023-04-18 | Janssen Pharmaceutica Nv | Anti-vista antibodies and fragments |
WO2015103438A2 (en) | 2014-01-02 | 2015-07-09 | Genelux Corporation | Oncolytic virus adjunct therapy with agents that increase virus infectivity |
EP3094352B1 (en) | 2014-01-16 | 2020-09-23 | Academia Sinica | Compositions and methods for treatment and detection of cancers |
WO2016114819A1 (en) | 2015-01-16 | 2016-07-21 | Academia Sinica | Compositions and methods for treatment and detection of cancers |
US10150818B2 (en) | 2014-01-16 | 2018-12-11 | Academia Sinica | Compositions and methods for treatment and detection of cancers |
JP6736467B2 (en) | 2014-02-04 | 2020-08-05 | ジェネンテック, インコーポレイテッド | Smoothing mutant and method of using the same |
WO2015127407A1 (en) | 2014-02-21 | 2015-08-27 | Stemcentrx, Inc. | Anti-dll3 antibodies and drug conjugates for use in melanoma |
RU2016141307A (en) | 2014-03-21 | 2018-04-24 | Регенерон Фармасьютикалз, Инк. | EXCELLENT HUMAN ANIMALS THAT MAKE SINGLE-DOMAIN BINDING PROTEINS |
DK3122869T3 (en) | 2014-03-24 | 2019-09-09 | Biogen Ma Inc | PROCEDURES FOR REDUCING GLUTAMINE DEPRESSION IN MAMMAL CULTURE CULTURE |
EP3129767B1 (en) | 2014-03-27 | 2021-09-01 | Academia Sinica | Reactive labelling compounds and uses thereof |
WO2015153765A1 (en) | 2014-04-01 | 2015-10-08 | Adimab, Llc | Multispecific antibody analogs comprising a common light chain, and methods of their preparation and use |
IL248511B (en) | 2014-05-13 | 2022-07-01 | Bavarian Nordic As | Combination therapy for treating cancer with a poxvirus expressing a tumor antigen and a monoclonal antibody against tim-3 |
US10118969B2 (en) | 2014-05-27 | 2018-11-06 | Academia Sinica | Compositions and methods relating to universal glycoforms for enhanced antibody efficacy |
TWI717319B (en) | 2014-05-27 | 2021-02-01 | 中央研究院 | Fucosidase from bacteroides and methods using the same |
TWI679020B (en) | 2014-05-27 | 2019-12-11 | 中央研究院 | Anti-her2 glycoantibodies and uses thereof |
KR20170003720A (en) | 2014-05-27 | 2017-01-09 | 아카데미아 시니카 | Anti-cd20 glycoantibodies and uses thereof |
KR102494193B1 (en) | 2014-05-28 | 2023-01-31 | 아카데미아 시니카 | Anti-tnf-alpha glycoantibodies and uses thereof |
TWI695011B (en) | 2014-06-18 | 2020-06-01 | 美商梅爾莎納醫療公司 | Monoclonal antibodies against her2 epitope and methods of use thereof |
HUE043847T2 (en) | 2014-08-28 | 2019-09-30 | Halozyme Inc | Combination therapy with a hyaluronan-degrading enzyme and an immune checkpoint inhibitor |
CN107001404B (en) | 2014-09-08 | 2021-06-29 | 中央研究院 | Activation of human iNKT cells using glycolipids |
EP3193932B1 (en) | 2014-09-15 | 2023-04-26 | F. Hoffmann-La Roche AG | Antibody formulations |
TWI701435B (en) | 2014-09-26 | 2020-08-11 | 日商中外製藥股份有限公司 | Method to determine the reactivity of FVIII |
MA40764A (en) | 2014-09-26 | 2017-08-01 | Chugai Pharmaceutical Co Ltd | THERAPEUTIC AGENT INDUCING CYTOTOXICITY |
TWI700300B (en) | 2014-09-26 | 2020-08-01 | 日商中外製藥股份有限公司 | Antibodies that neutralize substances with the function of FVIII coagulation factor (FVIII) |
MA41685A (en) | 2014-10-17 | 2017-08-22 | Biogen Ma Inc | COPPER SUPPLEMENT FOR THE REGULATION OF GLYCOSYLATION IN A MAMMAL CELL CULTURE PROCESS |
MA40864A (en) | 2014-10-31 | 2017-09-05 | Biogen Ma Inc | HYPOTAURINE, GABA, BETA-ALANINE AND CHOLINE FOR THE REGULATION OF THE ACCUMULATION OF RESIDUAL BY-PRODUCTS IN MAMMAL CELL CULTURE PROCESSES |
US10208120B2 (en) | 2014-11-05 | 2019-02-19 | Genentech, Inc. | Anti-FGFR2/3 antibodies and methods using same |
US11033637B2 (en) | 2014-11-21 | 2021-06-15 | University Of Maryland, Baltimore | Targeted structure-specific particulate delivery systems |
US11543411B2 (en) | 2014-12-05 | 2023-01-03 | Prelude Corporation | DCIS recurrence and invasive breast cancer |
US9975965B2 (en) | 2015-01-16 | 2018-05-22 | Academia Sinica | Compositions and methods for treatment and detection of cancers |
US10495645B2 (en) | 2015-01-16 | 2019-12-03 | Academia Sinica | Cancer markers and methods of use thereof |
CN107430127B (en) | 2015-01-24 | 2020-08-28 | 中央研究院 | Cancer markers and methods of use thereof |
JP6779887B2 (en) | 2015-01-24 | 2020-11-04 | アカデミア シニカAcademia Sinica | New glycan conjugate and how to use it |
KR102620346B1 (en) | 2015-01-30 | 2024-01-02 | 아카데미아 시니카 | Compositions and Methods Related to Universal Glycoforms for Enhanced Antibody Efficacy |
EP4177267A1 (en) | 2015-02-02 | 2023-05-10 | Children's Health Care d/b/a Children's Minnesota | Anti-surrogate light chain antibodies |
CA2975875A1 (en) | 2015-02-04 | 2016-08-11 | Genentech, Inc. | Mutant smoothened and methods of using the same |
WO2016149678A1 (en) | 2015-03-19 | 2016-09-22 | Regeneron Pharmaceuticals, Inc. | Non-human animals that select for light chain variable regions that bind antigen |
EP3279216A4 (en) | 2015-04-01 | 2019-06-19 | Chugai Seiyaku Kabushiki Kaisha | Method for producing polypeptide hetero-oligomer |
US10787500B2 (en) | 2015-04-10 | 2020-09-29 | Adimab, Llc | Methods for purifying heterodimeric multispecific antibodies from parental homodimeric antibody species |
EP3285811A1 (en) | 2015-04-21 | 2018-02-28 | Institut Gustave Roussy | Therapeutic methods, products and compositions inhibiting znf555 |
CN107922497B (en) | 2015-06-24 | 2022-04-12 | 詹森药业有限公司 | anti-VISTA antibodies and fragments |
TWI797060B (en) | 2015-08-04 | 2023-04-01 | 美商再生元醫藥公司 | Taurine supplemented cell culture medium and methods of use |
EP3344806A4 (en) | 2015-09-04 | 2019-03-20 | OBI Pharma, Inc. | Glycan arrays and method of use |
EP3398965A4 (en) | 2015-12-28 | 2019-09-18 | Chugai Seiyaku Kabushiki Kaisha | Method for promoting efficiency of purification of fc region-containing polypeptide |
WO2017117311A1 (en) | 2015-12-30 | 2017-07-06 | Genentech, Inc. | Formulations with reduced degradation of polysorbate |
US20170239355A1 (en) | 2015-12-30 | 2017-08-24 | Genentech, Inc. | Use of tryptophan derivatives for protein formulations |
WO2017136558A1 (en) | 2016-02-04 | 2017-08-10 | Curis, Inc. | Mutant smoothened and methods of using the same |
TWI756204B (en) | 2016-02-12 | 2022-03-01 | 比利時商楊森製藥公司 | Anti-vista antibodies and fragments, uses thereof, and methods of identifying same |
CA3016170A1 (en) | 2016-03-08 | 2017-09-14 | Academia Sinica | Methods for modular synthesis of n-glycans and arrays thereof |
EP3429693B1 (en) | 2016-03-15 | 2023-08-23 | Mersana Therapeutics, Inc. | Napi2b-targeted antibody-drug conjugates and methods of use thereof |
WO2017161206A1 (en) | 2016-03-16 | 2017-09-21 | Halozyme, Inc. | Conjugates containing conditionally active antibodies or antigen-binding fragments thereof, and methods of use |
CA3018365A1 (en) | 2016-03-23 | 2017-09-28 | The General Hospital Corporation | Assays and methods for detecting udp-glucose |
CA3019560A1 (en) | 2016-03-29 | 2017-10-05 | Obi Pharma, Inc. | Antibodies, pharmaceutical compositions and methods |
US10980894B2 (en) | 2016-03-29 | 2021-04-20 | Obi Pharma, Inc. | Antibodies, pharmaceutical compositions and methods |
WO2017172771A2 (en) | 2016-03-29 | 2017-10-05 | Janssen Biotech, Inc. | Method of treating psoriasis with increased interval dosing of anti-il12/23 antibody |
EP3440208B1 (en) * | 2016-04-06 | 2020-09-16 | Zumutor Biologics, Inc. | Vectors for cloning and expression of proteins, methods and applications thereof |
WO2017175058A1 (en) | 2016-04-07 | 2017-10-12 | Janssen Pharmaceutica Nv | Anti-vista antibodies and fragments, uses thereof, and methods of identifying same |
KR20230110820A (en) | 2016-04-22 | 2023-07-25 | 오비아이 파머 인코퍼레이티드 | Cancer immunotherapy by immune activation or immune modulation via globo series antigens |
WO2018022479A1 (en) | 2016-07-25 | 2018-02-01 | Biogen Ma Inc. | Anti-hspa5 (grp78) antibodies and uses thereof |
EP3490592A4 (en) | 2016-07-27 | 2020-03-25 | OBI Pharma, Inc. | Immunogenic/therapeutic glycan compositions and uses thereof |
WO2018023121A1 (en) | 2016-07-29 | 2018-02-01 | Obi Pharma, Inc. | Human antibodies, pharmaceutical compositions and methods |
JP7213549B2 (en) | 2016-08-22 | 2023-01-27 | シーエイチオー ファーマ インコーポレイテッド | Antibodies, Binding Fragments, and Methods of Use |
JP7125932B2 (en) | 2016-09-06 | 2022-08-25 | 中外製薬株式会社 | Methods of Using Bispecific Antibodies Recognizing Coagulation Factor IX and/or Activated Coagulation Factor IX and Coagulation Factor X and/or Activated Coagulation Factor X |
CA3037961A1 (en) | 2016-09-30 | 2018-04-05 | Janssen Biotech, Inc. | Safe and effective method of treating psoriasis with anti-il23 specific antibody |
KR20190078648A (en) | 2016-11-16 | 2019-07-04 | 얀센 바이오테크 인코포레이티드 | Methods for treating psoriasis with anti-IL23 specific antibodies |
EP3541414A4 (en) | 2016-11-21 | 2020-11-11 | OBI Pharma, Inc. | Conjugated biological molecules, pharmaceutical compositions and methods |
EP3573658A4 (en) | 2017-01-30 | 2021-07-21 | Janssen Biotech, Inc. | Anti-tnf antibodies, compositions, and methods for the treatment of active psoriatic arthritis |
JP2020506947A (en) | 2017-02-07 | 2020-03-05 | ヤンセン バイオテツク,インコーポレーテツド | Anti-TNF antibodies, compositions and methods for treating active ankylosing spondylitis |
TW201922780A (en) | 2017-09-25 | 2019-06-16 | 美商健生生物科技公司 | Safe and effective method of treating Lupus with anti-IL12/IL23 antibody |
BR112020005834A2 (en) | 2017-09-29 | 2020-09-24 | Chugai Seiyaku Kabushiki Kaisha | multispecific antigen binding molecule having blood clotting factor viii cofactor function (fviii) replacement activity, and pharmaceutical formulation containing said molecule as an active ingredient |
WO2019150309A1 (en) | 2018-02-02 | 2019-08-08 | Hammack Scott | Modulators of gpr68 and uses thereof for treating and preventing diseases |
EP3530282A1 (en) | 2018-02-27 | 2019-08-28 | Diaccurate | Therapeutic methods |
KR20200129125A (en) | 2018-03-05 | 2020-11-17 | 얀센 바이오테크 인코포레이티드 | How to treat Crohn's disease with anti-IL23 specific antibodies |
KR20200131838A (en) * | 2018-03-14 | 2020-11-24 | 에프. 호프만-라 로슈 아게 | Methods for affinity maturation of antibodies |
US11203645B2 (en) | 2018-06-27 | 2021-12-21 | Obi Pharma, Inc. | Glycosynthase variants for glycoprotein engineering and methods of use |
WO2020016838A2 (en) | 2018-07-18 | 2020-01-23 | Janssen Biotech, Inc. | Sustained response predictors after treatment with anti-il23 specific antibody |
EP3833389A1 (en) | 2018-08-08 | 2021-06-16 | Genentech, Inc. | Use of tryptophan derivatives and l-methionine for protein formulation |
WO2020056338A1 (en) | 2018-09-14 | 2020-03-19 | Prelude Corporation | Method of selection for treatment of subjects at risk of invasive breast cancer |
SI3883606T1 (en) | 2018-09-24 | 2023-10-30 | Janssen Biotech, Inc. | Safe and effective method of treating ulcerative colitis with anti-il12/il23 antibody |
CA3113818A1 (en) | 2018-10-05 | 2020-04-09 | Bavarian Nordic A/S | Combination therapy for treating cancer with an intravenous administration of a recombinant mva and an immune checkpoint antagonist or agonist |
WO2020086408A1 (en) | 2018-10-26 | 2020-04-30 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | A high-yield perfusion-based transient gene expression bioprocess |
US11548941B2 (en) | 2018-11-20 | 2023-01-10 | Janssen Biotech, Inc. | Safe and effective method of treating psoriasis with anti-IL-23 specific antibody |
SG11202104918PA (en) | 2018-11-20 | 2021-06-29 | Bavarian Nordic As | Therapy for treating cancer with an intratumoral and/or intravenous administration of a recombinant mva encoding 4-1bbl (cd137l) and/or cd40l |
EP3897722A4 (en) | 2018-12-18 | 2022-09-14 | Janssen Biotech, Inc. | Safe and effective method of treating lupus with anti-il12/il23 antibody |
EP3911676A1 (en) | 2019-01-15 | 2021-11-24 | Janssen Biotech, Inc. | Anti-tnf antibody compositions and methods for the treatment of juvenile idiopathic arthritis |
KR20210118878A (en) | 2019-01-23 | 2021-10-01 | 얀센 바이오테크 인코포레이티드 | Anti-TNF antibody composition for use in a method of treating psoriatic arthritis |
EP3693063A1 (en) | 2019-02-06 | 2020-08-12 | Diaccurate | Methods and compositions for treating cancer |
KR20210141998A (en) | 2019-03-14 | 2021-11-23 | 얀센 바이오테크 인코포레이티드 | Method of making anti-TNF antibody composition |
JP2022525145A (en) | 2019-03-14 | 2022-05-11 | ヤンセン バイオテツク,インコーポレーテツド | A production method for producing an anti-IL12 / IL23 antibody composition. |
MA55283A (en) | 2019-03-14 | 2022-01-19 | Janssen Biotech Inc | METHODS FOR PRODUCING ANTI-TNF ANTIBODY COMPOSITIONS |
MA55282A (en) | 2019-03-14 | 2022-01-19 | Janssen Biotech Inc | MANUFACTURING METHODS FOR THE PRODUCTION OF ANTI-TNF ANTIBODY COMPOSITIONS |
EA202192459A1 (en) | 2019-03-18 | 2021-11-25 | Янссен Байотек, Инк. | METHOD FOR TREATMENT OF PSORIASIS WITH ANTIBODY TO IL12 / IL23 IN CHILDREN |
SG11202111345PA (en) | 2019-04-19 | 2021-11-29 | Chugai Pharmaceutical Co Ltd | Chimeric receptor that recognizes engineered site in antibody |
KR20220012883A (en) | 2019-05-23 | 2022-02-04 | 얀센 바이오테크 인코포레이티드 | A method of treating inflammatory bowel disease with a combination therapy of IL-23 and an antibody against TNF alpha |
CA3142580A1 (en) | 2019-06-03 | 2020-12-10 | Janssen Biotech, Inc. | Anti-tnf antibodies, compositions, and methods for the treatment of active ankylosing spondylitis |
EP3976648A1 (en) | 2019-06-03 | 2022-04-06 | Janssen Biotech, Inc. | Anti-tnf antibody compositions, and methods for the treatment of psoriatic arthritis |
JPWO2021010326A1 (en) | 2019-07-12 | 2021-01-21 | ||
WO2021028752A1 (en) | 2019-08-15 | 2021-02-18 | Janssen Biotech, Inc. | Anti-tfn antibodies for treating type i diabetes |
EP4061406A1 (en) | 2019-11-20 | 2022-09-28 | Bavarian Nordic A/S | Recombinant mva viruses for intratumoral and/or intravenous administration for treating cancer |
WO2021239666A1 (en) | 2020-05-26 | 2021-12-02 | Diaccurate | Therapeutic methods |
CA3212729A1 (en) | 2021-03-12 | 2022-09-15 | Janssen Biotech, Inc. | Safe and effective method of treating psoriatic arthritis with anti-il23 specific antibody |
AU2022232007A1 (en) | 2021-03-12 | 2023-10-26 | Janssen Biotech, Inc. | Method of treating psoriatic arthritis patients with inadequate response to tnf therapy with anti-il23 specific antibody |
WO2023281462A1 (en) | 2021-07-09 | 2023-01-12 | Janssen Biotech, Inc. | Manufacturing methods for producing anti-tnf antibody compositions |
KR20240032991A (en) | 2021-07-09 | 2024-03-12 | 얀센 바이오테크 인코포레이티드 | Manufacturing Methods for Producing Anti-TNF Antibody Compositions |
US20230038355A1 (en) | 2021-07-09 | 2023-02-09 | Janssen Biotech, Inc. | Manufacturing Methods for Producing Anti-IL12/IL23 Antibody Compositions |
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AU6235294A (en) * | 1993-02-02 | 1994-08-29 | Scripps Research Institute, The | Methods for producing polypeptide binding sites |
AU6132994A (en) * | 1993-02-02 | 1994-08-29 | Scripps Research Institute, The | Methods for producing antibody libraries using universal or randomized immunoglobulin light chains |
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AU706343B2 (en) | 1999-06-17 |
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CA2198899C (en) | 2010-02-23 |
US6096551A (en) | 2000-08-01 |
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