CA2200952A1 - Novel compositions comprising quaternary ammonium compounds and neutral lipids for the introduction of polyanionic materials into cells - Google Patents

Novel compositions comprising quaternary ammonium compounds and neutral lipids for the introduction of polyanionic materials into cells

Info

Publication number
CA2200952A1
CA2200952A1 CA002200952A CA2200952A CA2200952A1 CA 2200952 A1 CA2200952 A1 CA 2200952A1 CA 002200952 A CA002200952 A CA 002200952A CA 2200952 A CA2200952 A CA 2200952A CA 2200952 A1 CA2200952 A1 CA 2200952A1
Authority
CA
Canada
Prior art keywords
cells
introduction
quaternary ammonium
neutral lipids
ammonium compounds
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002200952A
Other languages
French (fr)
Other versions
CA2200952C (en
Inventor
Steven Michial Ansell
Barbara Mui
Michael Hope
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Arbutus Biopharma Corp
Original Assignee
Inex Pharmaceuticals Corp.
Steven Michial Ansell
Barbara Mui
Michael Hope
Tekmira Pharmaceuticals Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/442,267 external-priority patent/US5753613A/en
Application filed by Inex Pharmaceuticals Corp., Steven Michial Ansell, Barbara Mui, Michael Hope, Tekmira Pharmaceuticals Corporation filed Critical Inex Pharmaceuticals Corp.
Publication of CA2200952A1 publication Critical patent/CA2200952A1/en
Application granted granted Critical
Publication of CA2200952C publication Critical patent/CA2200952C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/127—Liposomes
    • A61K9/1271—Non-conventional liposomes, e.g. PEGylated liposomes, liposomes coated with polymers
    • A61K9/1272—Non-conventional liposomes, e.g. PEGylated liposomes, liposomes coated with polymers with substantial amounts of non-phosphatidyl, i.e. non-acylglycerophosphate, surfactants as bilayer-forming substances, e.g. cationic lipids

Abstract

The present invention provides compositions and methods which are useful for the introduction of polyanionic materials into cells. The compositions are mixtures of cationic compounds and neutral lipids which are typically formulated as liposomes. The cationic compounds are quaternary ammonium compounds wherein the nitrogen has two attached long chain alkyl groups, at least one of which is unsaturated.
The methods for transfecting cells involve (a) contacting the polyanionic materials with the compositions above to form a polyanionic material-liposome complex, and (b) contacting the complex with the cells to be transfected.
CA002200952A 1994-09-30 1995-09-29 Novel compositions comprising quaternary ammonium compounds and neutral lipids for the introduction of polyanionic materials into cells Expired - Fee Related CA2200952C (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US31639994A 1994-09-30 1994-09-30
US08/442,267 1995-05-16
US08/316,399 1995-05-16
US08/442,267 US5753613A (en) 1994-09-30 1995-05-16 Compositions for the introduction of polyanionic materials into cells
PCT/CA1995/000553 WO1996010390A1 (en) 1994-09-30 1995-09-29 Novel compositions for the introduction of polyanionic materials into cells
US08/536,584 US5785992A (en) 1994-09-30 1995-09-29 Compositions for the introduction of polyanionic materials into cells

Publications (2)

Publication Number Publication Date
CA2200952A1 true CA2200952A1 (en) 1996-04-11
CA2200952C CA2200952C (en) 2006-04-11

Family

ID=26980411

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002200952A Expired - Fee Related CA2200952C (en) 1994-09-30 1995-09-29 Novel compositions comprising quaternary ammonium compounds and neutral lipids for the introduction of polyanionic materials into cells

Country Status (5)

Country Link
US (1) US5785992A (en)
EP (1) EP1179340A3 (en)
AT (1) ATE219660T1 (en)
CA (1) CA2200952C (en)
DE (1) DE69527206T2 (en)

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Also Published As

Publication number Publication date
US5785992A (en) 1998-07-28
CA2200952C (en) 2006-04-11
EP1179340A2 (en) 2002-02-13
ATE219660T1 (en) 2002-07-15
EP1179340A3 (en) 2003-05-07
DE69527206T2 (en) 2003-02-27
DE69527206D1 (en) 2002-08-01

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