CA2210247A1 - Modification of xylanase to improve thermophilicity, alkophilicity and thermostability - Google Patents

Modification of xylanase to improve thermophilicity, alkophilicity and thermostability

Info

Publication number
CA2210247A1
CA2210247A1 CA002210247A CA2210247A CA2210247A1 CA 2210247 A1 CA2210247 A1 CA 2210247A1 CA 002210247 A CA002210247 A CA 002210247A CA 2210247 A CA2210247 A CA 2210247A CA 2210247 A1 CA2210247 A1 CA 2210247A1
Authority
CA
Canada
Prior art keywords
xylanase
amino acids
thermophilicity
thermostability
alkalophilicity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002210247A
Other languages
French (fr)
Other versions
CA2210247C (en
Inventor
Kazuhiko Ishikawa
Makoto Yaguchi
Wing L. Sung
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Research Council of Canada
Original Assignee
National Research Council of Canada
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National Research Council of Canada filed Critical National Research Council of Canada
Publication of CA2210247A1 publication Critical patent/CA2210247A1/en
Application granted granted Critical
Publication of CA2210247C publication Critical patent/CA2210247C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01032—Xylan endo-1,3-beta-xylosidase (3.2.1.32), i.e. endo-1-3-beta-xylanase
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
    • D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
    • D21C5/005—Treatment of cellulose-containing material with microorganisms or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Abstract

Producing a xylanase enzyme of superior performance in the bleaching of pulp. More specifically, a modified xylanase of Family 11 that shows improved thermophilicity, alkalophilicity, and thermostability as compared to the naturalxylanase. The modified xylanases contain any of three types of modifications: (1) changing amino acids 10, 27, and 29 of Trichoderma reesei xylanase II or the corresponding amino acids of another Family 11 xylanase, where these amino acidsare changed to histidine, methionine, and leucine, respectively; (2) substitution of amino acids in the N-terminal region with amino acids from another xylanase enzyme. In a preferred embodiment, substitution of the natural Bacillus circulans or Trichoderma reesei xylanase with a short sequence of amino acids from Thermomonospora fusca xylanase yielded chimeric xylanases with higher thermophilicity and alkalophilicity; (3) an extension upstream of the N-terminus of up to 10 amino acids. In a preferred embodiment, extension of the N-terminus of the xylanase with the tripeptide glycine-arginine-arginine improved its performance.
CA002210247A 1996-09-09 1997-08-27 Modification of xylanase to improve thermophilicity, alkophilicity and thermostability Expired - Fee Related CA2210247C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/709,912 US5759840A (en) 1996-09-09 1996-09-09 Modification of xylanase to improve thermophilicity, alkalophilicity and thermostability
US08/709,912 1996-09-09

Publications (2)

Publication Number Publication Date
CA2210247A1 true CA2210247A1 (en) 1998-03-09
CA2210247C CA2210247C (en) 2002-11-26

Family

ID=24851808

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002210247A Expired - Fee Related CA2210247C (en) 1996-09-09 1997-08-27 Modification of xylanase to improve thermophilicity, alkophilicity and thermostability

Country Status (9)

Country Link
US (2) US5759840A (en)
EP (1) EP0828002B1 (en)
JP (1) JPH10179155A (en)
CN (2) CN100595276C (en)
AR (1) AR009574A1 (en)
BR (1) BR9706843A (en)
CA (1) CA2210247C (en)
DE (1) DE69712003D1 (en)
NZ (1) NZ328680A (en)

Families Citing this family (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5759840A (en) * 1996-09-09 1998-06-02 National Research Council Of Canada Modification of xylanase to improve thermophilicity, alkalophilicity and thermostability
US5871550A (en) * 1997-08-26 1999-02-16 Genencor International, Inc. Mutant Thermonospora spp. cellulase
DK1131447T4 (en) * 1998-11-16 2011-12-19 Ca Nat Research Council Thermostable xylanases
US7060482B1 (en) * 1998-11-16 2006-06-13 National Research Council Of Canada Thermostable xylanases
US6329186B1 (en) 1998-12-07 2001-12-11 Novozymes A/S Glucoamylases with N-terminal extensions
CN1329665A (en) * 1998-12-07 2002-01-02 诺维信公司 Gluocoamylases with N-terminal extensions
WO2000039289A2 (en) * 1998-12-23 2000-07-06 Danisco A/S Endo-beta-1,4-xylanase inhibitor aus weizenmehl und seine wirkung auf verschiedene xylanasen
US8372620B2 (en) 1998-12-23 2013-02-12 Dupont Nutrition Biosciences Aps Bacterial xylanases
EP1179075A2 (en) * 1999-05-12 2002-02-13 Xencor, Inc. Bacillus circulans xylanase mutants
US6682923B1 (en) 1999-05-12 2004-01-27 Xencor Thermostable alkaliphilic xylanase
US7718411B1 (en) 2004-08-05 2010-05-18 Danisco Us Inc. Trichoderma reesei G/11 xylanases with improved stability
FI108728B (en) 1999-10-12 2002-03-15 Carbozyme Oy Procedure for Improving Stability of Xylanases in the G / 11 Family and for Changing an Optimal pH Range
CA2395617C (en) 1999-12-30 2012-10-30 Genencor International, Inc. Trichoderma reesei xylanase
JP4795604B2 (en) * 2000-03-08 2011-10-19 ダニスコ エイ／エス Modified xylanase with altered sensitivity to xylanase inhibitors
AU2001267171A1 (en) * 2000-05-31 2001-12-11 National Research Council Of Canada Modified xylanases exhibiting increased thermophilicity and alkalophilicity
AU2002230784A1 (en) * 2000-11-10 2002-05-21 Xencor Novel thermostable alkaliphilic xylanase
WO2002052100A2 (en) * 2000-12-22 2002-07-04 Iogen Bio-Products Corporation Alkaline extraction stages comprising xylanase
AU2002229430A1 (en) * 2001-01-18 2002-07-30 Iogen Bio-Products Corporation Use of xylanase in pulp bleaching
US7510860B1 (en) * 2001-11-21 2009-03-31 National Research Council Of Canada Xylanases with enhanced thermophilicity and alkalophilicity
AUPR968801A0 (en) * 2001-12-21 2002-01-24 Unisearch Limited Improvements in enzyme stability
CA2477847A1 (en) * 2002-03-06 2003-09-12 Iogen Bio-Products Corporation Xylanase treatment of chemical pulp
ES2394708T3 (en) 2002-06-14 2013-02-05 Verenium Corporation Xylanases, nucleic acids that encode them and methods to make and purify them
US20040112555A1 (en) * 2002-12-03 2004-06-17 Jeffrey Tolan Bleaching stage using xylanase with hydrogen peroxide, peracids, or a combination thereof
CN108486086A (en) 2003-07-02 2018-09-04 维莱尼姆公司 Dextranase, encode they nucleic acid and preparation and use their method
WO2005021714A2 (en) 2003-08-11 2005-03-10 Diversa Corporation Laccases, nucleic acids encoding them and methods for making and using them
ES2501190T3 (en) * 2003-09-15 2014-10-01 Danisco Us Inc. Modified enzymes, methods to produce modified enzymes and uses thereof
US20070243595A1 (en) * 2006-04-14 2007-10-18 Clarkson Kathleen A Modified enzymes, methods to produce modified enzymes and uses thereof
CN100354424C (en) * 2003-11-17 2007-12-12 中国农业大学 Expression method for high temperature resistant xylanase and specific expression carrier for same
WO2005093072A1 (en) * 2004-03-25 2005-10-06 Iogen Bio-Products Corporation Modified xylanases exhibiting improved expression
CN100491533C (en) * 2005-04-13 2009-05-27 中国农业科学院饲料研究所 Improved xylanase with high specific activity, its gene expression vector and recombinant yeast cell, and expression method
ES2682284T3 (en) 2006-02-14 2018-09-19 Bp Corporation North America Inc. Xylanases, nucleic acids that encode them and methods to make and use them
US7723712B2 (en) * 2006-03-17 2010-05-25 Micron Technology, Inc. Reduced power consumption phase change memory and methods for forming the same
RU2464313C2 (en) * 2006-04-12 2012-10-20 Нэйшенл Рисерч Каунсил Оф Кэнэда Modified xylanase
WO2007115391A1 (en) * 2006-04-12 2007-10-18 National Research Council Of Cananda Modification of xylanases to increase thermophilicity, thermostability and alkalophilicity
CN101466879B (en) 2006-06-08 2011-08-17 加拿大国立研究院 Extraction of hemp fibers
EP2444413A1 (en) 2006-08-04 2012-04-25 Verenium Corporation Methods for oil or gas well drilling, washing and/or fracturing
CN100419069C (en) * 2006-08-29 2008-09-17 湖北大学 Hat-resistant base-resistant xylanase yeast engineering bacterium and preparation method of heat-resistant xylanase thereof
EP2617819B1 (en) 2006-09-21 2016-04-27 BASF Enzymes LLC Phytases, nucleic acids encoding them and methods for making and using them
CN101289668B (en) * 2007-04-16 2010-09-08 屏东科技大学 Mutant gene of xylanase in high acid-base reaction range and point mutation process thereof
CN110577945A (en) 2007-10-03 2019-12-17 维莱尼姆公司 xylanases, nucleic acids encoding them, and methods for making and using them
WO2010022518A1 (en) * 2008-08-29 2010-03-04 Iogen Energy Corporation Modified beta-glucosidases with improved stability
KR101221003B1 (en) 2008-12-12 2013-01-10 한국생명공학연구원 Methods for Modulating Thermostability and Acid Tolerance of Microbes
MX355638B (en) 2009-05-21 2018-03-26 Verenium Corp Phytases, nucleic acids encoding them and methods for making and using them.
CN102884182A (en) * 2010-03-03 2013-01-16 诺维信股份有限公司 Xylanase variants and polynucleotides encoding same
CN106834384B (en) 2011-11-25 2020-12-11 三井化学株式会社 Mutant xylanase, method for producing same, use thereof, and method for producing lignocellulose saccharide
CN103184205A (en) * 2011-12-29 2013-07-03 中国科学院微生物研究所 Xylanase S1xB and coding gene thereof
CN103184204A (en) * 2011-12-29 2013-07-03 中国科学院微生物研究所 Xylanase SoxB and coding gene thereof
CN103642774B (en) * 2013-11-13 2016-08-17 宁夏夏盛实业集团有限公司 Mixing neutral cellulase and preparation method thereof and the application in papermaking is pulled an oar
CN103627686B (en) * 2013-11-14 2015-03-18 青岛蔚蓝生物集团有限公司 Xylanase mutant and application thereof
JP2015167552A (en) * 2014-03-11 2015-09-28 花王株式会社 mutant xylanase
CA2950273C (en) 2014-05-30 2022-06-21 Novozymes A/S Variants of gh family 11 xylanase and polynucleotides encoding same
JP2016029908A (en) 2014-07-28 2016-03-07 本田技研工業株式会社 Thermostable xylanase of gh family 10
JP6354462B2 (en) 2014-08-29 2018-07-11 本田技研工業株式会社 Thermostable xylanase belonging to GH family 10
WO2016073610A1 (en) 2014-11-07 2016-05-12 Novozymes A/S Xylanase based bleach boosting
JP7135334B2 (en) * 2018-02-22 2022-09-13 株式会社豊田中央研究所 Xylanase and its use
CN114438057B (en) * 2022-03-16 2023-05-19 齐鲁工业大学 Heat-resistant alkali-resistant xylanase and application thereof
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
CN114807093B (en) * 2022-06-22 2022-09-27 中国农业科学院北京畜牧兽医研究所 Method for improving heat stability of xylanase and phytase by adding fusion peptide segment to C terminal
CN116064479B (en) * 2022-11-30 2024-04-09 山东龙昌动物保健品股份有限公司 Compound preparation containing xylanase mutant and bile acid and application thereof

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9018426D0 (en) * 1990-08-22 1990-10-03 Sandoz Ltd Improvements in or relating to novel compounds
DE4226528A1 (en) * 1992-08-11 1994-02-17 Roehm Gmbh Batteryl xylanase, process for their preparation and a suitable bacterial strain, plasmid with associated structural gene, and baking and baking processes for the production of bread and baked goods using the xylanase
US5405769A (en) * 1993-04-08 1995-04-11 National Research Council Of Canada Construction of thermostable mutants of a low molecular mass xylanase
EP0728197A1 (en) * 1993-11-05 1996-08-28 Cornell Research Foundation, Inc. THERMOSTABLE XYLANASE FROM A $i(THERMOMONOSPORA FUSCA) GENE
US5759840A (en) * 1996-09-09 1998-06-02 National Research Council Of Canada Modification of xylanase to improve thermophilicity, alkalophilicity and thermostability

Also Published As

Publication number Publication date
EP0828002A3 (en) 1998-09-16
JPH10179155A (en) 1998-07-07
DE69712003D1 (en) 2002-05-23
CN1177639A (en) 1998-04-01
NZ328680A (en) 1998-04-27
CN1550548A (en) 2004-12-01
CN100595276C (en) 2010-03-24
US5866408A (en) 1999-02-02
AR009574A1 (en) 2000-04-26
CA2210247C (en) 2002-11-26
EP0828002A2 (en) 1998-03-11
BR9706843A (en) 2004-03-23
CN1155700C (en) 2004-06-30
US5759840A (en) 1998-06-02
EP0828002B1 (en) 2002-04-17

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