CA2217325A1 - Nucleic acid detection and amplification by chemical linkage of oligonucleotides - Google Patents

Nucleic acid detection and amplification by chemical linkage of oligonucleotides

Info

Publication number
CA2217325A1
CA2217325A1 CA002217325A CA2217325A CA2217325A1 CA 2217325 A1 CA2217325 A1 CA 2217325A1 CA 002217325 A CA002217325 A CA 002217325A CA 2217325 A CA2217325 A CA 2217325A CA 2217325 A1 CA2217325 A1 CA 2217325A1
Authority
CA
Canada
Prior art keywords
probe
pair
amplification
template
pairs
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002217325A
Other languages
French (fr)
Other versions
CA2217325C (en
Inventor
David Segev
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bio Rad Laboratories Inc
Original Assignee
Bio-Rad Laboratories, Inc.
David Segev
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio-Rad Laboratories, Inc., David Segev filed Critical Bio-Rad Laboratories, Inc.
Publication of CA2217325A1 publication Critical patent/CA2217325A1/en
Application granted granted Critical
Publication of CA2217325C publication Critical patent/CA2217325C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Abstract

The invention is directed towards a method of amplifying target nucleic acids by using two oligonucleotide probe complement pairs.
Each member of the probe pair contains a chemical functionality group which permits linkage of the probes when the functionality groups are adjacent to one another following hybridization of the probe pairs to the template. One probe in each pair is composed of two regions, a first region which hybridizes to the target and which contains the chemical functionality group and a second, protecting region which prevents target independent joining as shown in the figure. The other probe in the pair contains the corresponding chemical functionality group. Upon joining of a first probe pair, an amplification can proceed in which the newly joined first probe pair can serve as a template for the second, complementary probe pair, which can in turn serve as a template for unjoined first probe pairs. This cyclic amplification is sensitive enough for a discriminative amplification of sequences which differ by merely a point mutation and therefore is suitable for point mutation detection and genotype determination as well as for the determination of the presence or absence of a specific nucleic acid in a sample.
CA002217325A 1995-05-01 1996-04-30 Nucleic acid detection and amplification by chemical linkage of oligonucleotides Expired - Lifetime CA2217325C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/431,527 1995-05-01
US08/431,527 US5843650A (en) 1995-05-01 1995-05-01 Nucleic acid detection and amplification by chemical linkage of oligonucleotides
PCT/US1996/006042 WO1996034984A1 (en) 1995-05-01 1996-04-30 Nucleic acid detection and amplification by chemical linkage of oligonucleotides

Publications (2)

Publication Number Publication Date
CA2217325A1 true CA2217325A1 (en) 1996-11-07
CA2217325C CA2217325C (en) 2007-11-27

Family

ID=23712327

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002217325A Expired - Lifetime CA2217325C (en) 1995-05-01 1996-04-30 Nucleic acid detection and amplification by chemical linkage of oligonucleotides

Country Status (7)

Country Link
US (1) US5843650A (en)
EP (1) EP0828856B1 (en)
JP (1) JP4216333B2 (en)
AU (1) AU5918396A (en)
CA (1) CA2217325C (en)
DE (1) DE69635612T2 (en)
WO (1) WO1996034984A1 (en)

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Also Published As

Publication number Publication date
US5843650A (en) 1998-12-01
JP4216333B2 (en) 2009-01-28
WO1996034984A1 (en) 1996-11-07
EP0828856B1 (en) 2005-12-21
EP0828856A1 (en) 1998-03-18
JPH11504517A (en) 1999-04-27
AU5918396A (en) 1996-11-21
DE69635612D1 (en) 2006-01-26
CA2217325C (en) 2007-11-27
DE69635612T2 (en) 2006-10-19
EP0828856A4 (en) 2001-11-21

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