CA2236616A1 - Detection of nucleic acids by fluorescence quenching - Google Patents

Detection of nucleic acids by fluorescence quenching

Info

Publication number
CA2236616A1
CA2236616A1 CA002236616A CA2236616A CA2236616A1 CA 2236616 A1 CA2236616 A1 CA 2236616A1 CA 002236616 A CA002236616 A CA 002236616A CA 2236616 A CA2236616 A CA 2236616A CA 2236616 A1 CA2236616 A1 CA 2236616A1
Authority
CA
Canada
Prior art keywords
restriction endonuclease
dyes
stranded
signal primer
recognition site
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002236616A
Other languages
French (fr)
Other versions
CA2236616C (en
Inventor
James G. Nadeau
J. Bruce Pitner
James L. Schram
C. Preston Linn
Glenn P. Vonk
G. Terrance Walker
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
Original Assignee
Becton Dickinson and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=25320311&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2236616(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co
Publication of CA2236616A1 publication Critical patent/CA2236616A1/en
Application granted granted Critical
Publication of CA2236616C publication Critical patent/CA2236616C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors

Abstract

Single-stranded signal primers are modified by linkage to two dyes which form a donor/acceptor dye pair. The two dyes are positioned in sufficiently close spatial proximity on the signal primer that the fluorescence of the first dye is quenched by the second dye. The signal primer may further comprise a restriction endonuclease recognition site (RERS) between the two dyes. As the signal primer is initially single-stranded and remains single-stranded in the absence of target, the restriction endonuclease recognition site is not cleavable or nickable by the restriction endonuclease. In the presence of target, however, signal primer and the restriction endonuclease recognition site are rendered double-stranded and cleavable or nickable by the restriction endonuclease. Cleavage or nicking separates the two dyes and a change in fluorescence due to decreased quenching is detected as an indication of the presence of the target sequence or of target sequence amplification.
CA002236616A 1997-05-13 1998-05-04 Detection of nucleic acids by fluorescence quenching Expired - Lifetime CA2236616C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/855,085 1997-05-13
US08/855,085 US5846726A (en) 1997-05-13 1997-05-13 Detection of nucleic acids by fluorescence quenching

Publications (2)

Publication Number Publication Date
CA2236616A1 true CA2236616A1 (en) 1998-11-13
CA2236616C CA2236616C (en) 2002-04-23

Family

ID=25320311

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002236616A Expired - Lifetime CA2236616C (en) 1997-05-13 1998-05-04 Detection of nucleic acids by fluorescence quenching

Country Status (5)

Country Link
US (3) US5846726A (en)
EP (1) EP0878554B2 (en)
JP (1) JP3477366B2 (en)
CA (1) CA2236616C (en)
DE (1) DE69837125T3 (en)

Families Citing this family (158)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6787304B1 (en) 1994-12-28 2004-09-07 Georgetown University Fluorometric assay for detecting nucleic acid cleavage
US20030165908A1 (en) * 1994-12-30 2003-09-04 Georgetown University Fluorometric assay for detecting nucleic acid cleavage
US6766183B2 (en) 1995-11-22 2004-07-20 Medtronic Minimed, Inc. Long wave fluorophore sensor compounds and other fluorescent sensor compounds in polymers
US7070925B1 (en) 1996-07-16 2006-07-04 Gen-Probe Incorporated Method for determining the presence of an RNA analyte in a sample using a modified oligonucleotide probe
DE69736667T2 (en) * 1996-07-16 2007-09-06 Gen-Probe Inc., San Diego PROCESS FOR THE DETECTION AND AMPLIFICATION OF NUCLEIC ACID SEQUENCES USING MODIFIED OLIGONUCLEOTIDES WITH INCREASED TARGET MELT TEMPERATURE (TM)
US5853990A (en) 1996-07-26 1998-12-29 Edward E. Winger Real time homogeneous nucleotide assay
US5846726A (en) * 1997-05-13 1998-12-08 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
GB9725237D0 (en) * 1997-11-29 1998-01-28 Secr Defence Amplification system
DE10011529T1 (en) 1998-05-01 2011-09-01 Gen-Probe Incorporated Automatic diagnostic analyzer and method
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
EP1092047B1 (en) * 1998-07-02 2009-08-26 Gen-Probe Incorporated Molecular torches
US6216049B1 (en) 1998-11-20 2001-04-10 Becton, Dickinson And Company Computerized method and apparatus for analyzing nucleic acid assay readings
DE60001531T2 (en) 1999-04-23 2003-10-02 Molecular Probes Inc XANTHENE DYES AND THEIR USE AS LUMINESCENT EXTINGUISHING COMPOUNDS
PT1471926E (en) 1999-06-01 2011-03-11 Baylor College Medicine Compositions and methods for the therapeutic use of an atonal-associated sequence
US6316200B1 (en) 2000-06-08 2001-11-13 Becton, Dickinson And Company Probes and methods for detection of nucleic acids
EP1061134A3 (en) * 1999-06-17 2003-01-08 Becton Dickinson and Company Oligonucleotides for amplification and detection of hemochromatosis genes
US20020025519A1 (en) * 1999-06-17 2002-02-28 David J. Wright Methods and oligonucleotides for detecting nucleic acid sequence variations
US20030165913A1 (en) 1999-06-17 2003-09-04 Sha-Sha Wang Methods for detecting nucleic acid sequence variations
US6379888B1 (en) 1999-09-27 2002-04-30 Becton, Dickinson And Company Universal probes and methods for detection of nucleic acids
US20040081959A9 (en) * 1999-12-08 2004-04-29 Epoch Biosciences, Inc. Fluorescent quenching detection reagents and methods
US6727356B1 (en) 1999-12-08 2004-04-27 Epoch Pharmaceuticals, Inc. Fluorescent quenching detection reagents and methods
AUPQ495700A0 (en) * 2000-01-05 2000-02-03 Johnson & Johnson Research Pty. Limited Method for concurrent amplification and real time detection of polymorphic nucleic acid sequences
US6221603B1 (en) 2000-02-04 2001-04-24 Molecular Dynamics, Inc. Rolling circle amplification assay for nucleic acid analysis
US6323337B1 (en) 2000-05-12 2001-11-27 Molecular Probes, Inc. Quenching oligonucleotides
EP1158449A3 (en) * 2000-05-19 2004-12-15 Becton Dickinson and Company Computerized method and apparatus for analyzing readings of nucleic acid assays
US6686188B2 (en) 2000-05-26 2004-02-03 Amersham Plc Polynucleotide encoding a human myosin-like polypeptide expressed predominantly in heart and muscle
US6656700B2 (en) 2000-05-26 2003-12-02 Amersham Plc Isoforms of human pregnancy-associated protein-E
US6258546B1 (en) 2000-06-23 2001-07-10 Becton, Dickinson And Company Detection of nucleic acid amplification
US7081527B2 (en) * 2000-09-12 2006-07-25 Gen-Probe Incorporated Compositions, methods and kits for determining the presence of Cryptosporidium parvum organisms in a test sample
US20030148303A1 (en) * 2000-09-19 2003-08-07 Nadeau James G. Universal probes and methods for detection of nucleic acids
DE10061166A1 (en) * 2000-11-30 2002-06-06 Attomol Gmbh Molekulare Diagno Method and kit for the direct detection of nucleotide sequences, amino acid sequences or antigens
WO2002066986A2 (en) * 2001-02-15 2002-08-29 Medtronic Minimed, Inc. Polymers functionalized with fluorescent boronate motifs
EP1321520A4 (en) * 2001-03-27 2003-09-03 Nat Inst Of Advanced Ind Scien Novel nucleic acid probe and novel method of assaying nucleic acids using the same
US6617136B2 (en) 2001-04-24 2003-09-09 3M Innovative Properties Company Biological sample processing methods and compositions that include surfactants
US20030017567A1 (en) * 2001-04-24 2003-01-23 3M Innovative Properties Company Biological sample processing methods and compositions that include surfactants
US7112423B2 (en) * 2001-07-15 2006-09-26 Keck Graduate Institute Nucleic acid amplification using nicking agents
WO2003008623A2 (en) * 2001-07-15 2003-01-30 Keck Graduate Institute Methylation analysis using nicking agents
US20060073530A1 (en) * 2001-08-15 2006-04-06 Olaf Schneewind Methods and compositions involving sortase B
US20030082547A1 (en) 2001-08-27 2003-05-01 Ewing Gregory J. Non-fluorescent quencher compounds and biomolecular assays
US20050032060A1 (en) * 2001-08-31 2005-02-10 Shishir Shah Arrays comprising pre-labeled biological molecules and methods for making and using these arrays
US7045361B2 (en) 2001-09-12 2006-05-16 Medtronic Minimed, Inc. Analyte sensing via acridine-based boronate biosensors
US7439346B2 (en) * 2001-10-12 2008-10-21 Perkinelmer Las Inc. Nucleic acids arrays and methods of use therefor
WO2003035864A1 (en) * 2001-10-26 2003-05-01 Matsushita Electric Industrial Co., Ltd. Method of detecting target nucleic acid and nucleic acid probe
US20030166282A1 (en) 2002-02-01 2003-09-04 David Brown High potency siRNAS for reducing the expression of target genes
EP2213737B1 (en) 2002-02-01 2012-11-07 Life Technologies Corporation Double-stranded oligonucleotides
US20060009409A1 (en) 2002-02-01 2006-01-12 Woolf Tod M Double-stranded oligonucleotides
US6916621B2 (en) * 2002-03-27 2005-07-12 Spectral Genomics, Inc. Methods for array-based comparitive binding assays
US7220544B2 (en) 2002-05-08 2007-05-22 Arkray, Inc. Method for detecting target nucleic acid
JP4796299B2 (en) 2002-08-12 2011-10-19 ジェンネレックス インコーポレイティッド Methods and compositions for poxviruses and cancer
WO2004026804A1 (en) * 2002-09-20 2004-04-01 Integrated Dna Technologies, Inc. Anthraquinone quencher dyes, their methods of preparation and use
WO2004092412A2 (en) 2003-03-31 2004-10-28 Roche Diagnostics Gmbh Compositions and methods for detecting certain flaviviruses, including members of the japanese encephalitis virus serogroup
JP5117719B2 (en) * 2003-04-18 2013-01-16 ベクトン・ディキンソン・アンド・カンパニー Immune amplification
CA2523016C (en) * 2003-04-25 2018-05-15 Becton, Dickinson And Company Detection of herpes simplex virus types 1 and 2 by nucleic acid amplification
EP1633893B1 (en) 2003-05-19 2012-01-25 Gen-Probe Incorporated Compositions, methods and kits for determining the presence of trichomonas vaginalis in a test sample
CN101415844B (en) * 2003-09-12 2012-11-07 贝克顿·迪金森公司 Assay for SARS coronavirus by amplification and detection of nucleocapsid RNA sequence
CA2538082A1 (en) * 2003-09-12 2005-03-24 Becton, Dickinson And Company Assay for sars coronavirus by amplification and detection of the replicase sequence
US20050118619A1 (en) * 2003-09-22 2005-06-02 Wensheng Xia Dark quenchers for fluorescence resonance energy transfer (FRET) in bioassays
CA2544178A1 (en) * 2003-10-30 2005-05-19 Tufts-New England Medical Center Prenatal diagnosis using cell-free fetal dna in amniotic fluid
WO2005049849A2 (en) 2003-11-14 2005-06-02 Integrated Dna Technologies, Inc. Fluorescence quenching azo dyes, their methods of preparation and use
JP2007534308A (en) * 2003-11-19 2007-11-29 アレロジック・バイオサイエンシズ・コーポレーション Oligonucleotides labeled with multiple fluorophores
CA2548205C (en) 2003-12-03 2017-06-27 Abbott Laboratories Double stranded linear nucleic acid probe and uses thereof
US20050233332A1 (en) * 2004-04-14 2005-10-20 Collis Matthew P Multiple fluorophore detector system
US7939251B2 (en) 2004-05-06 2011-05-10 Roche Molecular Systems, Inc. SENP1 as a marker for cancer
WO2005113834A2 (en) * 2004-05-20 2005-12-01 Quest Diagnostics Investments Incorporated Single label comparative hybridization
US20080311564A1 (en) * 2004-08-06 2008-12-18 Fort Thomas L Sequences and Methods for Detection of Cytomegalovirus
US7910753B2 (en) * 2004-09-10 2011-03-22 Anaspec Incorporated Cyanine dyes and their applications as luminescence quenching compounds
ES2345993T3 (en) 2004-09-14 2010-10-07 The Regents Of The University Of Colorado, A Body Corporate METHOD FOR TREATMENT WITH BUCINDOLOL BASED ON GENETIC ADDRESSING.
US20060073511A1 (en) 2004-10-05 2006-04-06 Affymetrix, Inc. Methods for amplifying and analyzing nucleic acids
WO2006081353A2 (en) * 2005-01-27 2006-08-03 Quest Diagnostics Investments Incorporated Rapid comparative genome hybridization
US9012142B2 (en) * 2005-02-15 2015-04-21 Georgetown University Sequence-specific detection of nucleotide sequences
US7601498B2 (en) 2005-03-17 2009-10-13 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection and associated technology
US7776567B2 (en) * 2005-03-17 2010-08-17 Biotium, Inc. Dimeric and trimeric nucleic acid dyes, and associated systems and methods
AU2006242201A1 (en) * 2005-05-02 2006-11-09 Stratagene California Oligonucleotide probe/primer compositions and methods for polynucleotide detection
AU2006251637B2 (en) * 2005-05-20 2012-06-14 Integrated Dna Technologies, Inc. Compounds and methods for labeling oligonucleotides
WO2006136621A1 (en) * 2005-06-16 2006-12-28 Biotools Biotechnological & Medical Laboratories, S.A. Nucleic acid detection method involving the direct generation of a measurable signal
US20060292576A1 (en) * 2005-06-23 2006-12-28 Quest Diagnostics Investments Incorporated Non-in situ hybridization method for detecting chromosomal abnormalities
US8980246B2 (en) 2005-09-07 2015-03-17 Sillajen Biotherapeutics, Inc. Oncolytic vaccinia virus cancer therapy
KR20090004839A (en) 2005-09-07 2009-01-12 제네렉스, 인코포레이티드 Systemic treatment of metastatic and/or systemically-disseminated cancers using gm-csf-expressing poxviruses
EP1937850B1 (en) 2005-10-27 2019-05-29 The President and Fellows of Harvard College Methods and compositions for labeling nucleic acids
JP2009514549A (en) 2005-11-07 2009-04-09 シーメンス・ヘルスケア・ダイアグノスティックス・インコーポレーテッド Chlamydiatrachomatis-specific oligonucleotide sequence
US20090068643A1 (en) * 2005-11-23 2009-03-12 Integrated Dna Technologies, Inc. Dual Function Primers for Amplifying DNA and Methods of Use
US8076074B2 (en) 2005-11-29 2011-12-13 Quest Diagnostics Investments Incorporated Balanced translocation in comparative hybridization
CA2638782C (en) 2006-04-07 2020-08-25 Siemens Healthcare Diagnostics Inc. Neisseria gonorrhoeae pivng gene specific oligonucleotide sequences
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
WO2008014485A2 (en) 2006-07-28 2008-01-31 California Institute Of Technology Multiplex q-pcr arrays
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
CA2921048C (en) 2006-09-15 2018-06-05 Ottawa Hospital Research Institute Oncolytic rhabdovirus
US7807359B2 (en) * 2006-12-01 2010-10-05 Quest Diagnostics Investments Incorporated Methods of detecting TPMT mutations
US7507539B2 (en) * 2007-07-30 2009-03-24 Quest Diagnostics Investments Incorporated Substractive single label comparative hybridization
US8093063B2 (en) * 2007-11-29 2012-01-10 Quest Diagnostics Investments Incorporated Assay for detecting genetic abnormalities in genomic nucleic acids
EP2644709B1 (en) 2008-04-30 2014-12-17 Integrated Dna Technologies, Inc. RNase-H-based assays utilizing modified RNA monomers
US8911948B2 (en) * 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
WO2009137369A1 (en) * 2008-05-03 2009-11-12 Tufts Medical Center, Inc. Neonatal salivary genomics
US9090948B2 (en) 2008-09-30 2015-07-28 Abbott Molecular Inc. Primers and probes for detecting human papillomavirus and human beta globin sequences in test samples
US8039794B2 (en) 2008-12-16 2011-10-18 Quest Diagnostics Investments Incorporated Mass spectrometry assay for thiopurine-S-methyl transferase activity and products generated thereby
US8945842B2 (en) 2009-01-14 2015-02-03 Becton, Dickinson And Company Assay for Trichomonas vaginalis by amplification and detection of Trichomonas vaginalis AP65-1 gene
EP2401388A4 (en) 2009-02-23 2012-12-05 Univ Georgetown Sequence-specific detection of nucleotide sequences
EP2789689B1 (en) 2009-06-29 2016-04-27 Luminex Corporation Chimeric primers with hairpin conformations and methods of using same
US9409983B2 (en) 2009-07-23 2016-08-09 The Board Of Trustess Of The University Of Illinois Methods and compositions involving PBEF inhibitors for lung inflammation conditions and diseases
US9512481B2 (en) 2009-09-11 2016-12-06 The Regents Of The University Of Colorado, A Body Corporate Polymorphisms in the PDE3A gene
KR101759888B1 (en) 2009-09-14 2017-07-20 신라젠(주) Oncolytic vaccinia virus combination cancer therapy
CN102630250A (en) 2009-09-25 2012-08-08 基因诊断测试公司 Multiplex (+/-) stranded arrays and assays for detecting chromosomal abnormalities associated with cancer and other diseases
US8916349B2 (en) 2009-11-23 2014-12-23 Becton, Dickinson And Company Assay method for target nucleic acid by signal amplification using probe hybridization and restriction
EP2510088B1 (en) 2009-12-10 2016-10-05 Ottawa Hospital Research Institute Oncolytic rhabdovirus
US8877437B1 (en) 2009-12-23 2014-11-04 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection
WO2011079273A2 (en) 2009-12-23 2011-06-30 Arca Biopharma, Inc. Methods and compositions for cardiovascular diseases and conditions
WO2011091330A1 (en) 2010-01-22 2011-07-28 Gen-Probe Incorporated Probes for detecting the presence of trichomonas vaginalis in a sample
AU2011230496B2 (en) 2010-03-26 2015-09-17 Integrated Dna Technologies, Inc. Methods for enhancing nucleic acid hybridization
US9506057B2 (en) 2010-03-26 2016-11-29 Integrated Dna Technologies, Inc. Modifications for antisense compounds
US20120108799A1 (en) 2010-09-07 2012-05-03 Integrated Dna Technologies, Inc. Modifications for Antisense Compounds
EP2749887A3 (en) 2010-07-23 2014-10-01 Beckman Coulter, Inc. System Or Method Of Including Analytical Units
US9919047B2 (en) 2011-01-04 2018-03-20 Sillajen, Inc. Generation of antibodies to tumor antigens and generation of tumor specific complement dependent cytotoxicity by administration of oncolytic vaccinia virus
WO2012158238A2 (en) 2011-02-28 2012-11-22 University Of Iowa Research Foundation Anti-müllerian hormone changes in pregnancy and prediction ofadverse pregnancy outcomes and gender
CA2829300A1 (en) 2011-03-08 2012-09-13 King Abdullah University Of Science And Technology Molecular biomarker set for early detection of ovarian cancer
WO2012135053A2 (en) 2011-03-25 2012-10-04 Integrated Dna Technologies, Inc. Rnase h-based assays utilizing modified rna monomers
EP2718427B1 (en) 2011-06-08 2017-01-11 Children's Hospital of Eastern Ontario Research Institute Inc. Compositions for glioblastoma treatment
US10040853B2 (en) 2011-09-09 2018-08-07 Fred Hutchinson Cancer Research Center Methods and compositions involving NKG2D inhibitors and cancer
EP2776843B1 (en) 2011-11-07 2019-03-27 Beckman Coulter, Inc. Centrifuge system and workflow
EP3373015A1 (en) 2011-11-07 2018-09-12 Beckman Coulter Inc. Aliquotter system and workflow
JP6062449B2 (en) 2011-11-07 2017-01-18 ベックマン コールター， インコーポレイテッド Specimen container detection
KR20140091032A (en) 2011-11-07 2014-07-18 베크만 컬터, 인코포레이티드 Magnetic damping for specimen transport system
EP2776848B1 (en) 2011-11-07 2019-12-25 Beckman Coulter, Inc. System and method for transporting sample containers
CN104040352B (en) 2011-11-07 2016-09-07 贝克曼考尔特公司 Mechanical arm
US9212381B2 (en) 2011-11-10 2015-12-15 President And Fellows Of Harvard College Methods and compositions for labeling polypeptides
EP2839031B1 (en) 2012-04-19 2017-03-29 Seegene, Inc. Detection of target nucleic acid sequence by pto cleavage and extension-dependent signaling oligonucleotide cleavage
US10125373B2 (en) 2013-01-22 2018-11-13 Arizona Board Of Regents On Behalf Of Arizona State University Geminiviral vector for expression of rituximab
CA2901501C (en) 2013-02-21 2023-03-07 Children's Hospital Of Eastern Ontario Research Institute Inc. Vaccine composition
EP3052193B1 (en) 2013-10-03 2018-01-31 Oklahoma Medical Research Foundation Biomarkers for systemic lupus erythematosus disease activity, and intensity and flare
US10428371B2 (en) 2014-03-31 2019-10-01 Kabushiki Kaisha Dnaform Fluorescent labeled single-stranded nucleic acid and use thereof
WO2016086227A2 (en) 2014-11-26 2016-06-02 The Regents Of The University Of California Therapeutic compositions comprising transcription factors and methods of making and using the same
US11180792B2 (en) 2015-01-28 2021-11-23 The Regents Of The University Of California Methods and compositions for labeling a single-stranded target nucleic acid
US9708647B2 (en) 2015-03-23 2017-07-18 Insilixa, Inc. Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
US9957393B2 (en) 2015-03-30 2018-05-01 Enzo Biochem, Inc. Monoazo dyes with cyclic amine as fluorescence quenchers
EP3124619B1 (en) 2015-07-31 2019-03-06 Menicon Co., Ltd Reagents, method and kit for across and within dog breed glaucoma diagnosis
US9499861B1 (en) 2015-09-10 2016-11-22 Insilixa, Inc. Methods and systems for multiplex quantitative nucleic acid amplification
WO2017155858A1 (en) 2016-03-07 2017-09-14 Insilixa, Inc. Nucleic acid sequence identification using solid-phase cyclic single base extension
EP3507381B1 (en) * 2016-09-02 2020-10-21 Base4 Innovation Limited Single nucleotide detection method and associated probes
US20200216883A1 (en) * 2016-09-06 2020-07-09 Base4 Innovation Limited Single nucleotide detection method and associated probes
CN109790566A (en) * 2016-09-20 2019-05-21 贝斯4创新公司 Mononucleotide detection method and relevant probe
US10427162B2 (en) 2016-12-21 2019-10-01 Quandx Inc. Systems and methods for molecular diagnostics
CN110462062A (en) 2017-01-26 2019-11-15 俄克拉荷马医学研究基金会 Systemic loupus erythematosus Disease Activity, intensity and the biomarker of acute attack
EP3625366A1 (en) 2017-05-15 2020-03-25 Base4 Innovation Limited Single nucleotide detection method and associated probes
US11028439B2 (en) 2017-09-27 2021-06-08 Abbott Molecular Inc. Assay for detecting hepatitis B virus (HBV)
WO2019070854A1 (en) 2017-10-03 2019-04-11 Abbott Molecular Inc. Assay for detecting hepatitis c virus (hcv)
EP3628059B1 (en) 2017-10-03 2023-04-12 Abbott Molecular Inc. Assay for detecting human immunodeficiency virus (hiv)
CA3088866A1 (en) 2018-01-09 2019-07-18 Abbott Molecular Inc. Assay for detecting chlamydia trachomatis, neisseria gonorrhoeae, trichomonas vaginalis, and mycoplasma genitalium
WO2019213619A1 (en) 2018-05-04 2019-11-07 Abbott Laboratories Hbv diagnostic, prognostic, and therapeutic methods and products
AU2019319203A1 (en) 2018-08-08 2021-03-11 Gen-Probe Incorporated Compositions, methods and kits for detecting
KR102554477B1 (en) 2018-10-18 2023-07-11 오클라호마 메디컬 리써치 화운데이션 Biomarkers for Systemic Lupus Erythematosus (SLE) Disease Activity Immunity Index to Characterize Disease Activity
AU2019371161A1 (en) 2018-10-31 2021-06-17 Arizona Board Of Regents On Behalf Of The University Of Arizona Biomarkers and methods of use for radiation-induced lung injury
WO2020132090A1 (en) 2018-12-18 2020-06-25 Abbott Molecular Inc. Assay for detecting human papilloma virus (hpv)
CN109694905A (en) * 2019-01-29 2019-04-30 深圳市亚辉龙生物科技股份有限公司 Detect the nucleic acid compositions of testing gene methylation and its detection method of kit and testing gene methylation
EP3937780A4 (en) 2019-03-14 2022-12-07 InSilixa, Inc. Methods and systems for time-gated fluorescent-based detection
CA3152309A1 (en) 2019-08-23 2021-03-04 Gen-Probe Incorporated Compositions, methods and kits for detecting treponema pallidum
WO2021133916A1 (en) 2019-12-23 2021-07-01 Abbott Laboratories Compositions and methods for detecting picobirnavirus
WO2021138325A1 (en) 2019-12-30 2021-07-08 Abbott Laboratories Compositions and methods for detecting bunyavirus
WO2024030985A1 (en) 2022-08-04 2024-02-08 Abbott Laboratories Assays for detecting monkeypox virus

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS613063A (en) * 1984-06-18 1986-01-09 Fujirebio Inc Measurement of polynucleotide utilizing luminous substance
US5118605A (en) * 1984-10-16 1992-06-02 Chiron Corporation Polynucleotide determination with selectable cleavage sites
JPH0515439A (en) * 1991-07-08 1993-01-26 Matsushita Electric Ind Co Ltd Control circuit for jar type rice cooker
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5547861A (en) * 1994-04-18 1996-08-20 Becton, Dickinson And Company Detection of nucleic acid amplification
WO1996021144A1 (en) * 1994-12-30 1996-07-11 Georgetown University Fluorometric assay for detecting nucleic acid cleavage
US5550025A (en) * 1995-07-19 1996-08-27 Becton, Dickinson And Company Detection of hydrophobic amplification products by extraction into an organic phase
US5846726A (en) * 1997-05-13 1998-12-08 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching

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EP0878554A3 (en) 2001-11-28
DE69837125T3 (en) 2017-08-31
JP3477366B2 (en) 2003-12-10
DE69837125D1 (en) 2007-04-05
JPH11123083A (en) 1999-05-11
DE69837125T2 (en) 2007-12-13
US5846726A (en) 1998-12-08
EP0878554B2 (en) 2017-03-22
US5919630A (en) 1999-07-06
US6054279A (en) 2000-04-25
CA2236616C (en) 2002-04-23
EP0878554A2 (en) 1998-11-18

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