CA2239099A1 - Methods for generating polynucleotides having desired characteristics by iterative selection and recombination - Google Patents

Methods for generating polynucleotides having desired characteristics by iterative selection and recombination

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Publication number
CA2239099A1
CA2239099A1 CA002239099A CA2239099A CA2239099A1 CA 2239099 A1 CA2239099 A1 CA 2239099A1 CA 002239099 A CA002239099 A CA 002239099A CA 2239099 A CA2239099 A CA 2239099A CA 2239099 A1 CA2239099 A1 CA 2239099A1
Authority
CA
Canada
Prior art keywords
recombination
methods
desired characteristics
iterative selection
generating polynucleotides
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002239099A
Other languages
French (fr)
Other versions
CA2239099C (en
Inventor
Willem P. C. Stemmer
Andreas Crameri
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Maxygen Inc
Original Assignee
Affymax Technologies N.V.
Willem P. C. Stemmer
Andreas Crameri
Maxygen, Inc.
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Priority claimed from US08/564,955 external-priority patent/US5811238A/en
Application filed by Affymax Technologies N.V., Willem P. C. Stemmer, Andreas Crameri, Maxygen, Inc. filed Critical Affymax Technologies N.V.
Publication of CA2239099A1 publication Critical patent/CA2239099A1/en
Application granted granted Critical
Publication of CA2239099C publication Critical patent/CA2239099C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/545—IL-1
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/86—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides, e.g. penicillinase (3.5.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/02—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amides (3.5.2)
    • C12Y305/02006—Beta-lactamase (3.5.2.6)
    • H—ELECTRICITY
    • H10—SEMICONDUCTOR DEVICES; ELECTRIC SOLID-STATE DEVICES NOT OTHERWISE PROVIDED FOR
    • H10N—ELECTRIC SOLID-STATE DEVICES NOT OTHERWISE PROVIDED FOR
    • H10N60/00—Superconducting devices
    • H10N60/10—Junction-based devices
    • H10N60/128—Junction-based devices having three or more electrodes, e.g. transistor-like structures
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/565—Complementarity determining region [CDR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)

Abstract

A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.
CA002239099A 1995-11-30 1996-12-02 Methods for generating polynucleotides having desired characteristics by iterative selection and recombination Expired - Fee Related CA2239099C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US08/564,955 1995-11-30
US08/564,955 US5811238A (en) 1994-02-17 1995-11-30 Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US08/621,859 1996-03-25
US08/621,859 US6117679A (en) 1994-02-17 1996-03-25 Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
PCT/US1996/019256 WO1997020078A1 (en) 1995-11-30 1996-12-02 Methods for generating polynucleotides having desired characteristics by iterative selection and recombination

Publications (2)

Publication Number Publication Date
CA2239099A1 true CA2239099A1 (en) 1997-06-05
CA2239099C CA2239099C (en) 2004-11-30

Family

ID=27073702

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002239099A Expired - Fee Related CA2239099C (en) 1995-11-30 1996-12-02 Methods for generating polynucleotides having desired characteristics by iterative selection and recombination

Country Status (11)

Country Link
US (8) US6117679A (en)
EP (4) EP1103606A3 (en)
JP (3) JP4015193B2 (en)
AT (2) ATE205877T1 (en)
AU (1) AU713952B2 (en)
CA (1) CA2239099C (en)
DE (4) DE69615406T3 (en)
DK (2) DK0911396T3 (en)
ES (2) ES2165124T3 (en)
IL (1) IL124675A0 (en)
WO (1) WO1997020078A1 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7458125B2 (en) * 2002-09-20 2008-12-02 Colgate-Palmolive Company Oral care implement
US8372636B2 (en) 1996-05-31 2013-02-12 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof

Families Citing this family (619)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US20060257890A1 (en) 1996-05-20 2006-11-16 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5837458A (en) * 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6395547B1 (en) 1994-02-17 2002-05-28 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6165793A (en) * 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6406855B1 (en) 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6808904B2 (en) 1994-06-16 2004-10-26 Syngenta Participations Ag Herbicide-tolerant protox genes produced by DNA shuffling
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
US5958672A (en) 1995-07-18 1999-09-28 Diversa Corporation Protein activity screening of clones having DNA from uncultivated microorganisms
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US6562594B1 (en) 1999-09-29 2003-05-13 Diversa Corporation Saturation mutagenesis in directed evolution
US6361974B1 (en) 1995-12-07 2002-03-26 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US7018793B1 (en) 1995-12-07 2006-03-28 Diversa Corporation Combinatorial screening of mixed populations of organisms
WO2000053744A2 (en) * 1999-03-09 2000-09-14 Diversa Corporation End selection in directed evolution
US20030215798A1 (en) 1997-06-16 2003-11-20 Diversa Corporation High throughput fluorescence-based screening for novel enzymes
US6352842B1 (en) 1995-12-07 2002-03-05 Diversa Corporation Exonucease-mediated gene assembly in directed evolution
US20020028443A1 (en) * 1999-09-27 2002-03-07 Jay M. Short Method of dna shuffling with polynucleotides produced by blocking or interrupting a synthesis or amplification process
US6764835B2 (en) 1995-12-07 2004-07-20 Diversa Corporation Saturation mutageneis in directed evolution
US5830696A (en) 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
US6358709B1 (en) 1995-12-07 2002-03-19 Diversa Corporation End selection in directed evolution
US6740506B2 (en) 1995-12-07 2004-05-25 Diversa Corporation End selection in directed evolution
US6939689B2 (en) 1995-12-07 2005-09-06 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US6238884B1 (en) 1995-12-07 2001-05-29 Diversa Corporation End selection in directed evolution
US6713279B1 (en) 1995-12-07 2004-03-30 Diversa Corporation Non-stochastic generation of genetic vaccines and enzymes
US6506602B1 (en) 1996-03-25 2003-01-14 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6096548A (en) 1996-03-25 2000-08-01 Maxygen, Inc. Method for directing evolution of a virus
AU4113797A (en) 1996-07-10 1998-02-02 Lonza A.G. Method of preparing (s) - or (r) -3,3,3-trifluoro-2-hydroxy-2- methylpropionic acid
US5955275A (en) 1997-02-14 1999-09-21 Arcaris, Inc. Methods for identifying nucleic acid sequences encoding agents that affect cellular phenotypes
US6025485A (en) 1997-02-14 2000-02-15 Arcaris, Inc. Methods and compositions for peptide libraries displayed on light-emitting scaffolds
US20070009930A1 (en) * 1996-12-18 2007-01-11 Maxygen, Inc. Methods and compositions for polypeptide engineering
KR100570935B1 (en) 1997-01-17 2006-04-13 맥시겐, 인크. Evolution of whole cells and organisms by recursive sequence recombination
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US7148054B2 (en) * 1997-01-17 2006-12-12 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
GB9701425D0 (en) 1997-01-24 1997-03-12 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
US6623922B1 (en) 1997-02-14 2003-09-23 Deltagen Proteomics Methods for identifying, characterizing, and evolving cell-type specific CIS regulatory elements
US6159688A (en) 1997-03-18 2000-12-12 Novo Nordisk A/S Methods of producing polynucleotide variants
US6159687A (en) 1997-03-18 2000-12-12 Novo Nordisk A/S Methods for generating recombined polynucleotides
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
DE69827172T2 (en) * 1997-04-09 2006-02-23 Danisco A/S Lipase and use thereof for improving doughs and pastries
US6046925A (en) * 1997-04-14 2000-04-04 The Regents Of The University Of California Photochromic fluorescent proteins and optical memory storage devices based on fluorescent proteins
DE19725371B4 (en) * 1997-06-16 2005-01-20 Bornholdt, Stefan, Dr. A method for accelerating the evolutionary optimization of biopolymers and for improving the biopolymers produced therewith
GB9712512D0 (en) * 1997-06-16 1997-08-20 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
DE19737562A1 (en) * 1997-08-28 1999-05-06 Otogene Biotechnologische Fors Method for the identification of interactions between proteins or peptides
GB9722131D0 (en) * 1997-10-20 1997-12-17 Medical Res Council Method
WO1999021979A1 (en) 1997-10-28 1999-05-06 Maxygen, Inc. Human papillomavirus vectors
EP1030861A4 (en) * 1997-10-31 2001-09-05 Maxygen Inc Modification of virus tropism and host range by viral genome shuffling
US6156509A (en) * 1997-11-12 2000-12-05 Genencor International, Inc. Method of increasing efficiency of directed evolution of a gene using phagemid
KR20010032861A (en) 1997-12-08 2001-04-25 캘리포니아 인스티튜트 오브 테크놀로지 Method for creating polynucleotide and polypeptide sequences
AR017831A1 (en) 1997-12-10 2001-10-24 Pioneer Hi Bred Int METHOD FOR ALTERING THE COMPOSITION OF AMINO ACIDS OF A NATIVE PROTEIN OF INTEREST, PREPARED PROTEIN, AND POLINUCLEOTIDE
AU3289199A (en) * 1998-02-11 1999-08-30 Maxygen, Inc. Antigen library immunization
EP1555316A3 (en) * 1998-02-11 2008-01-30 Maxygen, Inc. Antigen library immunization
US7390619B1 (en) * 1998-02-11 2008-06-24 Maxygen, Inc. Optimization of immunomodulatory properties of genetic vaccines
JP2002507392A (en) * 1998-02-11 2002-03-12 マキシジェン， インコーポレイテッド Optimization of immunomodulatory properties of gene vaccines
EP1518927A3 (en) * 1998-02-11 2005-06-22 Maxygen, Inc. Optimization of immunomodulatory properties of genetic vaccines
DE19805334A1 (en) * 1998-02-11 1999-08-12 Otogene Biotechnologische Fors Process for the development of an active pharmaceutical ingredient
US6541011B2 (en) 1998-02-11 2003-04-01 Maxygen, Inc. Antigen library immunization
EP1054987A1 (en) * 1998-02-17 2000-11-29 Zeneca Limited Insecticidal peptides
WO1999057128A1 (en) 1998-05-01 1999-11-11 Maxygen, Inc. Optimization of pest resistance genes using dna shuffling
US6902918B1 (en) 1998-05-21 2005-06-07 California Institute Of Technology Oxygenase enzymes and screening method
US7153655B2 (en) * 1998-06-16 2006-12-26 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence
JP2002517995A (en) 1998-06-17 2002-06-25 マキシジェン， インコーポレイテッド Method for producing a polynucleotide having desired properties
US6365408B1 (en) 1998-06-19 2002-04-02 Maxygen, Inc. Methods of evolving a polynucleotides by mutagenesis and recombination
WO2000000632A1 (en) * 1998-06-29 2000-01-06 Phylos, Inc. Methods for generating highly diverse libraries
ATE231186T1 (en) 1998-07-21 2003-02-15 Danisco GROCERIES
KR20010083086A (en) * 1998-07-28 2001-08-31 추후제출 Expression of Functional Eukaryotic Proteins
US6951719B1 (en) * 1999-08-11 2005-10-04 Proteus S.A. Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained
US20040191772A1 (en) * 1998-08-12 2004-09-30 Dupret Daniel Marc Method of shuffling polynucleotides using templates
US20020058249A1 (en) * 1998-08-12 2002-05-16 Venkiteswaran Subramanian Dna shuffling to produce herbicide selective crops
US20030104417A1 (en) * 1998-08-12 2003-06-05 Proteus S.A. Template-mediated, ligation-oriented method of nonrandomly shuffling polynucleotides
US6991922B2 (en) * 1998-08-12 2006-01-31 Proteus S.A. Process for in vitro creation of recombinant polynucleotide sequences by oriented ligation
JP2002522072A (en) * 1998-08-12 2002-07-23 マキシジェン， インコーポレイテッド DNA shuffling of monooxygenase gene for production of industrial chemicals.
US20030092023A1 (en) * 1998-08-12 2003-05-15 Daniel Dupret Method of shuffling polynucleotides using templates
EP1115862B1 (en) 1998-09-23 2009-07-15 ZymoGenetics, Inc. Cytokine receptor zalpha11
AU6510799A (en) * 1998-10-07 2000-04-26 Maxygen, Inc. Dna shuffling to produce nucleic acids for mycotoxin detoxification
WO2000028018A1 (en) 1998-11-10 2000-05-18 Maxygen, Inc. Modified adp-glucose pyrophosphorylase for improvement and optimization of plant phenotypes
AU2023700A (en) * 1998-11-10 2000-05-29 Maxygen, Inc. Modified ribulose 1,5-bisphosphate carboxylase/oxygenase
US6438561B1 (en) * 1998-11-19 2002-08-20 Navigation Technologies Corp. Method and system for using real-time traffic broadcasts with navigation systems
IT1303776B1 (en) * 1998-11-19 2001-02-23 S I S S A Scuola Internaz Supe PROCESS FOR THE PREPARATION OF GENOTECHE, OF POLYPEPTIDES USING THESE GENOTECHE AND THE OCTOPUS OBTAINED.
US6358712B1 (en) 1999-01-05 2002-03-19 Trustee Of Boston University Ordered gene assembly
DE60042730D1 (en) * 1999-01-05 2009-09-24 Univ Boston IMPROVED CLONING PROCESS
US20040005673A1 (en) * 2001-06-29 2004-01-08 Kevin Jarrell System for manipulating nucleic acids
TR200101998T2 (en) 1999-01-07 2002-06-21 Zymogenetics, Inc. Soluble receptor BR 43x2 and methods of use
US6368861B1 (en) 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6436675B1 (en) 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US20030054390A1 (en) * 1999-01-19 2003-03-20 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
EP1151409A1 (en) * 1999-01-18 2001-11-07 Maxygen, Inc. Methods of populating data stuctures for use in evolutionary simulations
US6917882B2 (en) * 1999-01-19 2005-07-12 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
CA2337949C (en) * 1999-01-18 2011-03-15 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
US6961664B2 (en) 1999-01-19 2005-11-01 Maxygen Methods of populating data structures for use in evolutionary simulations
US7873477B1 (en) 2001-08-21 2011-01-18 Codexis Mayflower Holdings, Llc Method and system using systematically varied data libraries
EP2253704B1 (en) * 1999-01-19 2015-08-19 Codexis Mayflower Holdings, LLC Oligonucleotide mediated nucleic acid recombination
US7702464B1 (en) 2001-08-21 2010-04-20 Maxygen, Inc. Method and apparatus for codon determining
US8457903B1 (en) 1999-01-19 2013-06-04 Codexis Mayflower Holdings, Llc Method and/or apparatus for determining codons
US20070065838A1 (en) * 1999-01-19 2007-03-22 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US7024312B1 (en) 1999-01-19 2006-04-04 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
KR20010042037A (en) * 1999-01-19 2001-05-25 맥시겐, 인크. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
IL138206A (en) * 1999-02-04 2011-06-30 Verenium Corp Methods for non-stochastic generation of progeny polypeptides and hybrid polynucleotides
US20090130718A1 (en) * 1999-02-04 2009-05-21 Diversa Corporation Gene site saturation mutagenesis
IL144657A0 (en) 1999-02-11 2002-06-30 Maxygen Inc High throughput mass spectrometry
JP3399518B2 (en) * 1999-03-03 2003-04-21 インターナショナル・ビジネス・マシーンズ・コーポレーション Semiconductor structure and method of manufacturing the same
EP1165775A2 (en) 1999-03-05 2002-01-02 Maxygen, Inc. Recombination of insertion modified nucleic acids
US6531316B1 (en) 1999-03-05 2003-03-11 Maxyag, Inc. Encryption of traits using split gene sequences and engineered genetic elements
KR100743640B1 (en) 1999-03-09 2007-07-27 지모제넥틱스, 인코포레이티드 Novel cytokine zalpha11 ligand
US8148110B2 (en) * 1999-03-15 2012-04-03 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions by β-lactamase reporter fragment complementation
MXPA00011569A (en) * 1999-03-26 2004-12-03 Diversa Corp Exonuclease-mediated nucleic acid reassembly in directed evolution.
AU2005225057A1 (en) * 1999-03-26 2005-12-01 Bp Corporation North America Inc. Synthetic ligation reassembly in directed evolution
US20040137515A1 (en) * 1999-06-21 2004-07-15 Gary Lynch Methods and device for in vitro detection and characterization of psychoactives using analysis of repetitive electrical activity in a neuronal sample
AU5880900A (en) * 1999-06-25 2001-01-31 Maxygen, Inc. Methods and compositions for engineering of attenuated vaccines
DE60045931D1 (en) 1999-07-07 2011-06-16 Zymogenetics Inc Human receptor for cytokines
WO2001004287A1 (en) * 1999-07-07 2001-01-18 Maxygen Aps A method for preparing modified polypeptides
AU778837B2 (en) 1999-08-12 2004-12-23 Zoetis Services Llc Streptomyces avermitilis gene directing the ratio of B2:B1 avermectins
US6797510B1 (en) 2001-05-24 2004-09-28 Lexicon Genetics Incorporated Human kinases and polynucleotides encoding the same
US20080050809A1 (en) * 1999-09-28 2008-02-28 Alejandro Abuin Novel human kinases and polynucleotides encoding the same
US6734010B2 (en) 2001-05-09 2004-05-11 Lexicon Genetics Incorporated Human kinases and polynucleotides encoding the same
US6734009B2 (en) 2000-12-27 2004-05-11 Lexicon Genetics Incorporated Human kinases and polynucleotides encoding the same
US6759527B2 (en) 2001-03-20 2004-07-06 Lexicon Genetics Incorporated Human kinase and polynucleotides encoding the same
US6586230B1 (en) * 2000-10-27 2003-07-01 Lexicon Genetics Incorporated Human kinase and polynucleotides encoding the same
US20040002474A1 (en) * 1999-10-07 2004-01-01 Maxygen Inc. IFN-alpha homologues
US7430477B2 (en) * 1999-10-12 2008-09-30 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
AU7646000A (en) * 1999-11-05 2001-05-14 Novozymes A/S Methods to screen microorganisms or gene libraries for products secreted from a cell
DE19953854C2 (en) 1999-11-09 2002-01-17 Max Planck Gesellschaft Process for the production of biopolymers with modified properties
US6686515B1 (en) 1999-11-23 2004-02-03 Maxygen, Inc. Homologous recombination in plants
US7115712B1 (en) * 1999-12-02 2006-10-03 Maxygen, Inc. Cytokine polypeptides
WO2001046422A1 (en) 1999-12-23 2001-06-28 Zymogenetics, Inc. Novel cytokine zcyto18
EP2133098A1 (en) 2000-01-10 2009-12-16 Maxygen Holdings Ltd G-CSF conjugates
SG121902A1 (en) * 2000-01-11 2006-05-26 Maxygen Inc Integrated systems for diversity generation and screening
EE200200443A (en) 2000-02-09 2003-12-15 Basf Aktiengesellschaft A novel elongase gene and a method for obtaining polyunsaturated fatty acids
RU2278123C2 (en) 2000-02-11 2006-06-20 Максиджен Холдингз Лтд. Molecules similar to factor vii or viia
AU2001241939A1 (en) * 2000-02-28 2001-09-12 Maxygen, Inc. Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation
US7323299B2 (en) * 2000-03-21 2008-01-29 Neugenesis Corporation Methods for in vivo diversification of single genes
AU2001287273A1 (en) * 2000-03-24 2001-10-08 Maxygen, Inc. Methods for modulating cellular and organismal phenotypes
AU4964101A (en) * 2000-03-30 2001-10-15 Maxygen Inc In silico cross-over site selection
EP1749888B1 (en) 2000-04-05 2010-01-27 ZymoGenetics, Inc. Soluble zalpha11 cytokine receptors
US7115403B1 (en) 2000-05-16 2006-10-03 The California Institute Of Technology Directed evolution of galactose oxidase enzymes
US6479262B1 (en) 2000-05-16 2002-11-12 Hercules, Incorporated Solid phase enzymatic assembly of polynucleotides
US20030032059A1 (en) * 2000-05-23 2003-02-13 Zhen-Gang Wang Gene recombination and hybrid protein development
EP1283877A2 (en) * 2000-05-23 2003-02-19 California Institute Of Technology Gene recombination and hybrid protein development
US6686188B2 (en) * 2000-05-26 2004-02-03 Amersham Plc Polynucleotide encoding a human myosin-like polypeptide expressed predominantly in heart and muscle
WO2002000897A2 (en) 2000-06-23 2002-01-03 Maxygen, Inc. Novel chimeric promoters
AU2001272978A1 (en) * 2000-06-23 2002-01-08 Maxygen, Inc. Novel co-stimulatory molecules
EP2287296A1 (en) 2000-06-26 2011-02-23 Novozymes A/S Lipolytic enzyme
ES2593002T3 (en) 2000-06-26 2016-12-05 Zymogenetics, Inc. ZCYTOR17 cytokine receptor
US7846733B2 (en) * 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
WO2002004629A2 (en) * 2000-07-07 2002-01-17 Maxygen, Inc. Molecular breeding of transposable elements
US6994963B1 (en) 2000-07-10 2006-02-07 Ambion, Inc. Methods for recombinatorial nucleic acid synthesis
US6858422B2 (en) * 2000-07-13 2005-02-22 Codexis, Inc. Lipase genes
AU2001280968A1 (en) * 2000-07-31 2002-02-13 Menzel, Rolf Compositions and methods for directed gene assembly
WO2002016583A2 (en) * 2000-08-24 2002-02-28 Maxygen, Inc. Constructs and their use in metabolic pathway engineering
EP2157183A1 (en) 2000-08-25 2010-02-24 BASF Plant Science GmbH Plant polynucleotides encoding prenyl proteases
AU9120801A (en) * 2000-09-15 2002-03-26 Verenium Corporation Combinatorial screening of mixed populations of organisms
WO2002031129A2 (en) 2000-10-12 2002-04-18 Lexicon Genetics Incorporated Human kinases and polynucleotides encoding the same
US6912470B2 (en) * 2001-05-21 2005-06-28 Ecopia Biosciences, Inc. Genes and proteins involved in the biosynthesis of enediyne ring structures
US7045283B2 (en) 2000-10-18 2006-05-16 The Regents Of The University Of California Methods of high-throughput screening for internalizing antibodies
AU2004260931B9 (en) 2003-04-29 2012-01-19 E.I. Du Pont De Nemours And Company Novel glyphosate-N-acetyltransferase (GAT) genes
US7202070B2 (en) * 2000-10-31 2007-04-10 Biocatalytics, Inc. Method for reductive amination of a ketone using a mutated enzyme
AU2002228633A1 (en) * 2000-11-20 2002-06-03 Lexicon Genetics Incorporated Human kinases and polynucleotides encoding the same
GB2370038B (en) * 2000-12-12 2003-03-26 Bioinvent Int Ab A method for in vitro molecular evolution of protein function
JP2004530417A (en) * 2000-12-12 2004-10-07 レキシコン・ジェネティクス・インコーポレーテッド Novel human kinase and polynucleotide encoding it
JP4430303B2 (en) * 2000-12-12 2010-03-10 アリゲーター・バイオサイエンス・アーベー In vitro molecular evolution methods of protein function
US6958213B2 (en) 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function
CA2430329A1 (en) * 2000-12-13 2002-06-20 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
US20020150895A1 (en) * 2000-12-22 2002-10-17 Raymond Wheeler Method and apparatus for filtering and extending RNA alignment coverage
US20020086292A1 (en) 2000-12-22 2002-07-04 Shigeaki Harayama Synthesis of hybrid polynucleotide molecules using single-stranded polynucleotide molecules
JP2004524835A (en) * 2001-01-23 2004-08-19 レキシコン・ジェネティクス・インコーポレーテッド Novel human kinase and polynucleotide encoding it
US8008459B2 (en) * 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
US7838287B2 (en) * 2001-01-25 2010-11-23 Evolva Sa Library of a collection of cells
AU2002249170B2 (en) * 2001-02-02 2007-02-08 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V Method for identifying functional nucleic acids
US20050003354A1 (en) * 2001-02-21 2005-01-06 Maxygen, Inc. Methods for improving or altering promoter/enhancer properties
ATE361996T1 (en) * 2001-03-09 2007-06-15 Nugen Technologies Inc METHODS AND COMPOSITIONS FOR DUPLICATION OF RNA SEQUENCES
US20030087254A1 (en) * 2001-04-05 2003-05-08 Simon Delagrave Methods for the preparation of polynucleotide libraries and identification of library members having desired characteristics
EP1383879A4 (en) * 2001-04-06 2005-05-11 Lexicon Genetics Inc Novel human kinases and polynucleotides encoding the same
EP1470219A4 (en) 2001-04-16 2005-10-05 California Inst Of Techn Peroxide-driven cytochrome p450 oxygenase variants
AU2002258997A1 (en) * 2001-04-24 2002-11-05 Li-Cor, Inc. Polymerases with charge-switch activity and methods of generating such polymerases
EP1383887A4 (en) * 2001-05-03 2004-07-07 Rensselaer Polytech Inst Novel methods of directed evolution
NZ528260A (en) 2001-05-18 2005-09-30 Danisco Method of improving dough and bread quality with the addition of an enzyme that hydrolyses a glycolipid and a phospholipid and incapable of hydrolysing a triglyceride or monoglyceride
IL158487A0 (en) * 2001-05-18 2004-05-12 Wisconsin Alumni Res Found Method for the synthesis of dna sequences
DK2277997T3 (en) 2001-06-26 2013-12-02 Novozymes As Polypeptides with cellobiohydrolase I activity and the same coding polynucleotides
US20030143612A1 (en) * 2001-07-18 2003-07-31 Pointilliste, Inc. Collections of binding proteins and tags and uses thereof for nested sorting and high throughput screening
US7226768B2 (en) 2001-07-20 2007-06-05 The California Institute Of Technology Cytochrome P450 oxygenases
ATE432981T1 (en) * 2001-07-26 2009-06-15 Stratagene California MULTI-SITE MUTAGENesis
CA2391131C (en) * 2001-07-26 2004-10-12 Ecopia Biosciences Inc. Genes and proteins for rosaramicin biosynthesis
US6833441B2 (en) * 2001-08-01 2004-12-21 Abmaxis, Inc. Compositions and methods for generating chimeric heteromultimers
US20040078837A1 (en) * 2001-08-02 2004-04-22 Shannon Mark E. Four human zinc-finger-containing proteins: MDZ3, MDZ4, MDZ7 and MDZ12
US6943001B2 (en) * 2001-08-03 2005-09-13 Diversa Corporation Epoxide hydrolases, nucleic acids encoding them and methods for making and using them
EP1578987A2 (en) * 2001-08-03 2005-09-28 Diversa Corporation Epoxide hydrolases, nucleic acids encoding them and methods for making and using them
US6987079B2 (en) * 2001-08-14 2006-01-17 W.R. Grace & Co.-Conn. Supported catalyst systems
US7647184B2 (en) * 2001-08-27 2010-01-12 Hanall Pharmaceuticals, Co. Ltd High throughput directed evolution by rational mutagenesis
EP1961293A3 (en) 2001-09-27 2009-01-28 Pioneer Hi-Bred International, Inc. Phytate polynucleotides and methods of use
EP2093286B1 (en) 2001-10-01 2013-02-27 Dyax Corporation Multi-chain eukaryotic display vectors and uses thereof
ES2538342T3 (en) 2001-10-10 2015-06-19 Ratiopharm Gmbh Remodeling and glycoconjugation of follicle stimulating hormone (FSH)
EP2305311A3 (en) 2001-10-10 2011-07-20 BioGeneriX AG Glycoconjugation of peptides
AU2004236174B2 (en) 2001-10-10 2011-06-02 Novo Nordisk A/S Glycopegylation methods and proteins/peptides produced by the methods
WO2003035842A2 (en) * 2001-10-24 2003-05-01 Dyax Corporation Hybridization control of sequence variation
US7175983B2 (en) * 2001-11-02 2007-02-13 Abmaxis, Inc. Adapter-directed display systems
ES2334338T3 (en) 2001-11-05 2010-03-09 Zymogenetics, Inc. IL-21 ANTAGONISTS.
GB0126887D0 (en) * 2001-11-08 2002-01-02 Univ London Method for producing and identifying soluble protein domains
EP1456416B1 (en) 2001-11-19 2009-03-11 Parallele Bioscience, Inc. Multiplex oligonucleotide addition and target amplification
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
US6768476B2 (en) * 2001-12-05 2004-07-27 Etenna Corporation Capacitively-loaded bent-wire monopole on an artificial magnetic conductor
US20040137547A1 (en) * 2001-12-28 2004-07-15 Medtronic Minimed, Inc. Method for formulating a glucose oxidase enzyme with a desired property or properties and a glucose oxidase enzyme with the desired property
US20040009498A1 (en) * 2002-01-14 2004-01-15 Diversa Corporation Chimeric antigen binding molecules and methods for making and using them
WO2003061570A2 (en) * 2002-01-16 2003-07-31 Zyomyx, Inc. Engineered binding proteins
PT1576112E (en) 2002-01-18 2012-05-25 Zymogenetics Inc Cytokine receptor zcytor17 multimers
ATE401390T1 (en) 2002-01-18 2008-08-15 Zymogenetics Inc CYTOKINE (ZCYTOR17 LIGAND)
CA2472030A1 (en) * 2002-01-24 2003-07-31 Pointilliste, Inc. Use of collections of binding sites for sample profiling and other applications
EP1472349B1 (en) * 2002-01-25 2008-10-29 Evolva AG Methods for multiple parameter screening and evolution of cells to produce small molecules with multiple functionalities
US20040096826A1 (en) * 2002-01-30 2004-05-20 Evans Glen A. Methods for creating recombination products between nucleotide sequences
BRPI0307620B8 (en) 2002-02-12 2016-09-13 Pah Usa 15 Llc polynucleotide molecules encoding avec variants, methods for producing a recombinant streptomyces avermitilis strain, as well as said recombinant cells and avermectin composition
EP2428588B1 (en) 2002-02-21 2016-08-10 Alere San Diego, Inc. Recombinase Polymerase Amplification
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
PT1478738E (en) 2002-02-22 2009-02-16 Progenics Pharm Inc Anti-ccr5 antibody
US20030166010A1 (en) * 2002-02-25 2003-09-04 Affholter Joseph A. Custom ligand design for biomolecular filtration and purification for bioseperation
US20030224404A1 (en) * 2002-02-25 2003-12-04 Manuel Vega High throughput directed evolution of nucleic acids by rational mutagenesis
US7879582B2 (en) * 2002-02-26 2011-02-01 E. I. Du Pont De Nemours And Company Method for the recombination of genetic elements
US7220563B2 (en) 2002-02-27 2007-05-22 California Institute Of Technology Glucose 6-oxidases
US6977162B2 (en) * 2002-03-01 2005-12-20 Ravgen, Inc. Rapid analysis of variations in a genome
US7747391B2 (en) * 2002-03-01 2010-06-29 Maxygen, Inc. Methods, systems, and software for identifying functional biomolecules
US20050084907A1 (en) 2002-03-01 2005-04-21 Maxygen, Inc. Methods, systems, and software for identifying functional biomolecules
NZ535045A (en) * 2002-03-01 2008-04-30 Ravgen Inc Rapid analysis of variations in a genome
WO2003075129A2 (en) 2002-03-01 2003-09-12 Maxygen, Inc. Methods, systems, and software for identifying functional bio-molecules
EP1488335A4 (en) 2002-03-09 2006-11-15 Maxygen Inc Optimization of crossover points for directed evolution
ATE405676T1 (en) * 2002-03-11 2008-09-15 Nugen Technologies Inc METHOD FOR GENERATING DOUBLE STRANDED DNA WITH A 3' SINGLE STRAND PORTION AND USES OF THESE COMPLEXES FOR RECOMBINATION
DE60332711D1 (en) 2002-03-22 2010-07-08 Bayer Bioscience Nv NEW INSECTICIDES PROTEINS FROM BAZILLUS THURINGIENSIS
MXPA04009778A (en) 2002-04-08 2004-12-13 Pionner Hi Bred International Enhanced silk exsertion under stress.
WO2003089587A2 (en) * 2002-04-16 2003-10-30 Promega Corporation Method to enhance homologous recombination
CN102174546A (en) 2002-04-19 2011-09-07 维莱尼姆公司 Phospholipases, nucleic acids encoding them and methods for making and using them
US7226771B2 (en) 2002-04-19 2007-06-05 Diversa Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
CN101676728A (en) 2002-04-19 2010-03-24 津莫吉尼蒂克斯公司 cytokine receptor
IL164687A0 (en) * 2002-05-02 2005-12-18 Univ North Carolina Cellular libraries and methods for the preparationthereof
FR2839317A1 (en) * 2002-05-06 2003-11-07 Entomed Preparation of recombinant nucleic acid and derived proteins, useful e.g. as antibacterial agents, by shuffling partial sequences encoding several parent proteins
US20070178478A1 (en) * 2002-05-08 2007-08-02 Dhallan Ravinder S Methods for detection of genetic disorders
US7442506B2 (en) * 2002-05-08 2008-10-28 Ravgen, Inc. Methods for detection of genetic disorders
US7727720B2 (en) * 2002-05-08 2010-06-01 Ravgen, Inc. Methods for detection of genetic disorders
GB2388604B (en) * 2002-05-17 2005-01-26 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
JP4372679B2 (en) * 2002-05-17 2009-11-25 アリゲーター・バイオサイエンス・アーベー In vitro molecular evolution of protein function
ATE546150T1 (en) 2002-06-07 2012-03-15 Zymogenetics Inc USE OF IL-21 AND MONOCLONAL ANTIBODIES TO TREAT SOLID TUMORS
GB0213816D0 (en) * 2002-06-14 2002-07-24 Univ Aston Method of producing DNA and protein libraries
WO2003106639A2 (en) * 2002-06-14 2003-12-24 Dyax Corporation Recombination of nucleic acid library members
US9321832B2 (en) 2002-06-28 2016-04-26 Domantis Limited Ligand
DE10233047A1 (en) * 2002-07-19 2004-02-26 Amaxa Gmbh Preparing synthetic polypeptides, particularly fluorescent proteins, useful in pharmaceutical compositions, by aligning sequences of known proteins to define an average sequence
EP1527173A1 (en) * 2002-07-23 2005-05-04 Nuevolution A/S Gene shuffing by template switching
AU2003256794B2 (en) 2002-07-25 2008-07-31 Bio-Rad Laboratories, Inc. Hybrid protein methods and compositions
EP1529110B1 (en) * 2002-08-01 2011-10-05 Evolva Ltd. Methods of mixing large numbers of heterologous genes
BR0313281A (en) 2002-08-06 2007-07-24 Verdia Inc ap1 amine oxidase variants
US20060020396A1 (en) * 2002-09-09 2006-01-26 Rene Gantier Rational directed protein evolution using two-dimensional rational mutagenesis scanning
AU2003263552A1 (en) * 2002-09-09 2004-03-29 Nautilus Biotech Rational evolution of cytokines for higher stability, the cytokines and encoding nucleic acid molecules
US20050202438A1 (en) * 2002-09-09 2005-09-15 Rene Gantier Rational directed protein evolution using two-dimensional rational mutagenesis scanning
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
MXPA05003047A (en) * 2002-09-20 2005-05-27 Colgate Palmolive Co Toothbrush with gripping area.
AU2002951899A0 (en) * 2002-10-04 2002-10-24 Macquarie University Random drift mutagenesis
JP3447009B1 (en) * 2002-10-29 2003-09-16 實 平垣 Construct structure and method for producing the same
AU2003291677A1 (en) * 2002-10-30 2004-05-25 Pointilliste, Inc. Methods for producing polypeptide-tagged collections and capture systems containing the tagged polypeptides
US7135290B2 (en) * 2003-04-12 2006-11-14 Solazyme, Inc. Methods and compositions for evolving hydrogenase genes
US7090979B2 (en) * 2002-11-22 2006-08-15 The Regents Of The University Of California Derivatized versions of ligase enzymes for constructing DNA sequences
AU2003291962A1 (en) 2002-12-20 2004-07-14 Novozymes A/S Polypeptides having cellobiohydrolase ii activity and polynucleotides encoding same
CN102021205A (en) 2003-01-17 2011-04-20 丹尼斯科公司 Method of producing a protein ester and a protein subunit ester
US7955814B2 (en) 2003-01-17 2011-06-07 Danisco A/S Method
US20050196766A1 (en) * 2003-12-24 2005-09-08 Soe Jorn B. Proteins
MXPA05007654A (en) 2003-01-17 2005-09-30 Danisco Method.
JP2007525148A (en) * 2003-01-21 2007-09-06 エコピア バイオサイエンシーズ インク Polyene polyketides, methods for their production and use as pharmaceuticals [Related Applications] This application is based on US Provisional Application No. 60 / 441,123, filed Jan. 21, 2003, and US Provisional Application No. 60 / 441,2003. Claims priority to 60 / 494,568, US provisional application 60 / 469,810 filed May 13, 2003 and US provisional application 60 / 491,516 filed August 1, 2003 Is.
DE102004002243A1 (en) * 2003-02-07 2004-09-16 Trikon Technologies Limited, Newport Substrate e.g. silicon wafer, processing apparatus for use in e.g. power device, has dark space shield on zone side of chuck circumjacent and preventing presence of plasma between shield and periphery of substrate
AU2003290561A1 (en) * 2003-02-10 2004-09-06 Dana Ault-Riche Self-assembling arrays and uses thereof
EP3023498B1 (en) 2003-03-06 2018-11-28 BASF Enzymes LLC Amylases, nucleic acids encoding them and methods for making and using them
DK2853593T3 (en) 2003-03-07 2018-01-08 Dsm Ip Assets Bv Hydrolases, nucleic acids encoding them, and processes for their preparation and use
WO2004090115A2 (en) 2003-04-04 2004-10-21 Stratagene Renilla gfp mutants with increased fluorescent intensity and special shift
DK1613733T3 (en) 2003-04-04 2015-08-31 Basf Enzymes Llc PEKTATLYASER, nucleic acids encoding them, and methods for making and using the
CA2521084A1 (en) * 2003-04-14 2004-10-28 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
WO2005007808A2 (en) 2003-05-15 2005-01-27 Chiron Corporation Hiv polynucleotides and polypeptides derived from botswana mj4
EP1639091B1 (en) 2003-06-17 2012-12-05 California University Of Technology Regio- and enantioselective alkane hydroxylation with modified cytochrome p450
WO2005017106A2 (en) 2003-06-17 2005-02-24 California Institute Of Technology Libraries of optimized cytochrome p450 enzymes and the optimized p450 enzymes
US20050048578A1 (en) * 2003-06-26 2005-03-03 Epitomics, Inc. Methods of screening for monoclonal antibodies with desirable activity
BRPI0412279A (en) 2003-07-02 2006-09-19 Diversa Corp glucanases, nucleic acids encoding the same and methods for preparing and applying them
EP1668113B1 (en) 2003-08-11 2013-06-19 Verenium Corporation Laccases, nucleic acids encoding them and methods for making and using them
EP1660646B1 (en) 2003-08-11 2014-12-31 California Institute Of Technology Thermostable peroxide-driven cytochrome p450 oxygenase variants and methods of use
CA2536635C (en) 2003-08-25 2015-05-12 Funzyme Biotechnologies Sa Novel fungal proteins and nucleic acids encoding same
EP1670932B1 (en) 2003-08-27 2011-03-30 Proterec Ltd Libraries of recombinant chimeric proteins
US20070149496A1 (en) * 2003-10-31 2007-06-28 Jack Tuszynski Water-soluble compound
GB0716126D0 (en) 2007-08-17 2007-09-26 Danisco Process
US7906307B2 (en) 2003-12-24 2011-03-15 Danisco A/S Variant lipid acyltransferases and methods of making
US7718408B2 (en) 2003-12-24 2010-05-18 Danisco A/S Method
DK2119771T3 (en) 2003-12-24 2018-12-10 Dupont Nutrition Biosci Aps PROTEINS
WO2005082077A2 (en) 2004-02-25 2005-09-09 Pioneer Hi-Bred International, Inc. Novel bacillus thuringiensis crystal polypeptides, polynucleotides, and compositions thereof
CN1922314B (en) 2004-02-25 2010-05-05 诺维信公司 Fungall cell wall degrading enzyme
GB0405637D0 (en) 2004-03-12 2004-04-21 Danisco Protein
US7622125B2 (en) 2004-05-05 2009-11-24 Novartis Vaccines And Diagnostics, Inc. Polycistronic HIV vector constructs
AU2015203225B2 (en) * 2004-06-01 2017-05-04 Abbott Diagnostics Scarborough, Inc. Recombinase Polymerase Amplification
JP5026958B2 (en) * 2004-06-01 2012-09-19 アリーア サン ディエゴ， インコーポレイテッド Recombinase polymerase amplification
AR049214A1 (en) 2004-06-09 2006-07-05 Pioneer Hi Bred Int PEPTIDES OF TRANSIT TO PLASTIDES
WO2005123956A2 (en) * 2004-06-09 2005-12-29 Wisconsin Alumni Research Foundation Rapid production of oligonucleotides
EA013993B1 (en) 2004-06-16 2010-08-30 Верениум Корпорейшн Compositions and methods for enzymatic de-colorization of chlorophyll
MX2007000626A (en) 2004-07-16 2007-03-07 Danisco Protein.
GB0421598D0 (en) 2004-09-29 2004-10-27 Cambridge Advanced Tech Modification of plant development and morphology
JP2008523786A (en) * 2004-10-18 2008-07-10 コドン デバイシズ インコーポレイテッド Method for assembling high fidelity synthetic polynucleotides
US20060204979A1 (en) * 2004-12-01 2006-09-14 Invitrogen Corporation Reverse two-hybrid system for identification of interaction domains
DE102005005438A1 (en) * 2005-02-05 2006-08-24 Bayer Healthcare Ag Mutants of the fluorescent protein CGFPs, as well as their use
CA2596390A1 (en) 2005-02-08 2006-08-17 Zymogenetics, Inc. Anti-il-20, anti-il-22 and anti-il-22ra antibodies and binding partners and methods of using in inflammation
CA2614769A1 (en) 2005-03-10 2006-09-21 Verenium Corporation Lyase enzymes, nucleic acids encoding them and methods for making and using them
CA2611859C (en) 2005-03-15 2015-03-31 Verenium Corporation Cellulases, nucleic acids encoding them and methods for making and using them
US8715988B2 (en) 2005-03-28 2014-05-06 California Institute Of Technology Alkane oxidation by modified hydroxylases
US11214817B2 (en) 2005-03-28 2022-01-04 California Institute Of Technology Alkane oxidation by modified hydroxylases
US20070009928A1 (en) * 2005-03-31 2007-01-11 Lathrop Richard H Gene synthesis using pooled DNA
US8076108B2 (en) * 2005-04-26 2011-12-13 Cargill, Incorporated Polypeptides and biosynthetic pathways for the production of stereoisomers of monatin and their precursors
EP1891107B1 (en) 2005-05-12 2011-07-06 ZymoGenetics, Inc. Compositions and methods for modulating immune responses
PL2314623T3 (en) 2005-06-21 2012-11-30 Xoma Us Llc IL-1beta binding antibodies and fragments thereof
WO2007096702A2 (en) 2005-07-25 2007-08-30 Asm Scientific, Inc. Methods for multiplexing recombinase polymerase amplification
CA2621267A1 (en) 2005-09-07 2007-03-15 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
JP2009519224A (en) * 2005-11-18 2009-05-14 グロスター ファーマシューティカルズ， インコーポレイテッド Metabolite derivatives of HDAC inhibitor FK228
GB2432366B (en) * 2005-11-19 2007-11-21 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
JP2009523024A (en) * 2006-01-11 2009-06-18 アイクスイクスアエンメ・エッセ・ピ・ア Luminescent transgenic non-human animals, progeny, cell derivatives and their use
US20090324574A1 (en) 2006-02-02 2009-12-31 Verenium Corporation Esterases and Related Nucleic Acids and Methods
MY160772A (en) 2006-02-10 2017-03-15 Verenium Corp Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them
PL1989302T3 (en) 2006-02-14 2019-03-29 Bp Corp North America Inc Xylanases, nucleic acids encoding them and methods for making and using them
EP2112227B1 (en) 2006-03-07 2013-05-15 Cargill, Incorporated Aldolases, nucleic acids encoding them and methods for making and using them
EP3153580A3 (en) 2006-03-07 2017-04-19 BASF Enzymes LLC Aldolases, nucleic acids encoding them and methods for making and using them
US20070231805A1 (en) * 2006-03-31 2007-10-04 Baynes Brian M Nucleic acid assembly optimization using clamped mismatch binding proteins
WO2008013589A2 (en) * 2006-04-24 2008-01-31 Gloucester Pharmaceuticals Treatment of ras-expressing tumors
EP3088533B1 (en) 2006-05-04 2018-01-17 Alere San Diego, Inc. Recombinase polymerase amplification
WO2007137163A2 (en) 2006-05-19 2007-11-29 Georgia Tech Research Corporation Abc transporter ligand
WO2007146730A2 (en) * 2006-06-08 2007-12-21 Gloucester Pharmaceuticals Deacetylase inhibitor therapy
US7572618B2 (en) 2006-06-30 2009-08-11 Bristol-Myers Squibb Company Polynucleotides encoding novel PCSK9 variants
US8324158B2 (en) 2006-07-14 2012-12-04 Georgia Tech Research Corporation Methods for inhibiting CLC-2 channel with GATX2
AU2007356171B8 (en) 2006-08-04 2014-01-16 Bp Corporation North America Inc. Glucanases, nucleic acids encoding them, and methods for making and using them
US8252559B2 (en) 2006-08-04 2012-08-28 The California Institute Of Technology Methods and systems for selective fluorination of organic molecules
EP2069513A4 (en) 2006-08-04 2012-03-21 California Inst Of Techn Methods and systems for selective fluorination of organic molecules
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
MX2009003032A (en) 2006-09-21 2009-11-18 Verenium Corp Phytases, nucleic acids encoding them and methods for making and using them.
CN101558154B (en) 2006-09-21 2016-02-24 帝斯曼知识产权资产管理有限公司 Phospholipid hydrolase, encode their nucleic acid and methods for making and using same thereof
AU2007335208B8 (en) 2006-12-20 2014-08-28 Fibria Celulose S/A Nucleic acid molecules encoding plant proteins in the C3HC4 family and methods for the alteration of plant cellulose and lignin content
AU2007335207B2 (en) 2006-12-20 2012-08-02 Fibria Celulose S/A Nucleic acid constructs and methods for altering plant fiber length and/or plant height
PL2479267T3 (en) 2006-12-21 2017-06-30 Basf Enzymes Llc Amylases and glucoamylases, nucleic acids encoding them and methods for making and using them
EP2102230A2 (en) * 2006-12-29 2009-09-23 Gloucester Pharmaceuticals, Inc. Purifiction of romidepsin
CN103497238A (en) * 2006-12-29 2014-01-08 细胞基因公司 Romidepsin preparation
DE102007003143A1 (en) 2007-01-16 2008-07-17 Henkel Kgaa New alkaline protease from Bacillus gibsonii and detergents and cleaners containing this novel alkaline protease
JP5124593B2 (en) 2007-01-25 2013-01-23 デュポン ニュートリション バイオサイエンシーズ エーピーエス Production of lipid acyltransferase from transformed Bacillus licheniformis cells
BRPI0807132A2 (en) 2007-01-30 2018-12-04 Syngenta Participations Ag enzymes for the treatment of lignocellulosics, nucleic acids encoding them, and methods and use thereof
US20090118130A1 (en) 2007-02-12 2009-05-07 Codexis, Inc. Structure-activity relationships
EP2124988B1 (en) 2007-02-23 2014-11-12 The Trustees of Columbia University in the City of New York Methods to activate or block the hla-e/qa-1 restricted cd8+ t cell regulatory pathway to treat immunological disease
US7883879B2 (en) 2007-10-01 2011-02-08 Codexis, Inc. Ketoreductase polypeptides for the production of azetidinone
NZ601191A (en) 2007-10-03 2014-01-31 Verenium Corp Xylanases, nucleic acids encoding them and methods for making and using them
AU2008308509B2 (en) 2007-10-04 2014-10-23 Zymogenetics, Inc. B7 family member zB7H6 and related compositions and methods
DE102007051092A1 (en) 2007-10-24 2009-04-30 Henkel Ag & Co. Kgaa Subtilisin from Becillus pumilus and detergents and cleaners containing this new subtilisin
JP5298134B2 (en) 2007-11-01 2013-09-25 パーシード セラピューティクス リミテッド ライアビリティ カンパニー Immunosuppressive polypeptides and nucleic acids
US20110262508A1 (en) 2007-11-07 2011-10-27 Paul Michael Watt Antimicrobial compositions, formulations and uses thereof
US8129586B2 (en) 2007-11-20 2012-03-06 Pioneer Hi-Bred International, Inc. Maize ethylene signaling genes and modulation of same for improved stress tolerance in plants
JP5474809B2 (en) 2007-11-21 2014-04-16 ロスキレ ユニバーシティ Polypeptide with ice-binding activity
WO2009065934A1 (en) * 2007-11-22 2009-05-28 Novozymes A/S Polypeptides having ferulic acid esterase activity and polynucleotides encoding same
MX2010007014A (en) 2007-12-21 2010-09-30 Danisco Process for edible oil refining using a lipid acyltransferase.
EP2865750A3 (en) 2008-01-03 2015-08-05 BASF Enzymes LLC Transferases and oxidoreductases, nucleic acids encoding them and methods for making and using them
US8541220B2 (en) 2008-01-03 2013-09-24 Verenium Corporation Isomerases, nucleic acids encoding them and methods for making and using them
JP5727792B2 (en) 2008-01-25 2015-06-03 オーフス ユニバーシテ Selective exosite inhibition of PAPP-A activity against IGFBP-4
US8034568B2 (en) * 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
DK2250595T3 (en) 2008-02-12 2017-09-04 Codexis Inc PROCEDURE FOR SELECTING AN OPTIMIZED VARIETY OF VARIETIES
WO2009102901A1 (en) * 2008-02-12 2009-08-20 Codexis, Inc. Method of generating an optimized, diverse population of variants
WO2009117698A2 (en) 2008-03-21 2009-09-24 Nugen Technologies, Inc. Methods of rna amplification in the presence of dna
WO2009127729A1 (en) * 2008-04-17 2009-10-22 Novozymes A/S Polypeptides having ferulic acid esterase activity and polynucleotides encoding same
GB0807161D0 (en) * 2008-04-18 2008-05-21 Danisco Process
EP3115459A3 (en) 2008-05-23 2017-04-26 E. I. du Pont de Nemours and Company Novel dgat genes for increased seed storage lipid production and altered fatty acid profiles in oilseed plants
US8383346B2 (en) 2008-06-13 2013-02-26 Codexis, Inc. Combined automated parallel synthesis of polynucleotide variants
US8178333B2 (en) 2008-06-24 2012-05-15 Codexis, Inc. Biocatalytic processes for the preparation of substantially stereomerically pure fused bicyclic proline compounds
US8426178B2 (en) * 2008-08-27 2013-04-23 Codexis, Inc. Ketoreductase polypeptides for the production of a 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine
US8288131B2 (en) * 2008-08-27 2012-10-16 Codexis, Inc. Ketoreductase polypeptides and uses thereof
WO2010025287A2 (en) * 2008-08-27 2010-03-04 Codexis, Inc. Ketoreductase polypeptides for the production of 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine
SI2329014T1 (en) 2008-08-29 2015-01-30 Codexis, Inc. Ketoreductase polypeptides for the stereoselective production of (4s)-3š(5s)-5(4-fluorophenyl)-5-hydroxypentanoylć-4-phenyl-1,3-oxazolidin-2-one
US8357503B2 (en) 2008-08-29 2013-01-22 Bunge Oils, Inc. Hydrolases, nucleic acids encoding them and methods for making and using them
US8153391B2 (en) 2008-08-29 2012-04-10 Bunge Oils, Inc. Hydrolases, nucleic acids encoding them and methods for making and using them
US8198062B2 (en) 2008-08-29 2012-06-12 Dsm Ip Assets B.V. Hydrolases, nucleic acids encoding them and methods for making and using them
WO2010033237A2 (en) * 2008-09-22 2010-03-25 Calmune Corporation Methods for creating diversity in libraries and libraries, display vectors and methods, and displayed molecules
KR102025502B1 (en) 2008-09-26 2019-09-25 토카겐 인크. Gene therapy vectors and cytosine deaminases
AU2009313551B2 (en) 2008-11-07 2015-12-17 Fabrus Llc Anti-DLL4 antibodies and uses thereof
US8247192B2 (en) 2008-11-10 2012-08-21 Codexis, Inc. Penicillin-G acylases
US9394333B2 (en) 2008-12-02 2016-07-19 Wave Life Sciences Japan Method for the synthesis of phosphorus atom modified nucleic acids
SG196784A1 (en) 2008-12-03 2014-02-13 Scripps Research Inst Stem cell cultures
CA2743707A1 (en) 2008-12-04 2010-06-10 Pioneer Hi-Bred International, Inc. Methods and compositions for enhanced yield by targeted expression of knotted1
US8187856B2 (en) * 2008-12-18 2012-05-29 Codexis, Inc. Recombinant halohydrin dehalogenase polypeptides
SG172783A1 (en) 2008-12-25 2011-08-29 Codexis Inc Enone reductases
JP2012517801A (en) 2009-02-13 2012-08-09 ノバルティス アーゲー Biosynthetic cluster nucleic acid molecules encoding non-ribosomal peptide synthases and uses thereof
CA2752818A1 (en) 2009-02-26 2010-09-02 Codexis, Inc. Beta-glucosidase variant enzymes and related polynucleotides
EP2401366B1 (en) 2009-02-26 2013-12-18 Codexis, Inc. Transaminase biocatalysts
US8716553B2 (en) 2009-03-02 2014-05-06 Pioneer Hi Bred International Inc NAC transcriptional activators involved in abiotic stress tolerance
BRPI1013886A2 (en) 2009-03-17 2016-10-11 Codexis Inc endoglucanase variants, polynucleotides and related uses
WO2010109053A1 (en) 2009-03-27 2010-09-30 Proyeto De Biomedicina Cima, S.L. Methods and compositions for the treatment of cirrhosis and liver fibrosis
EP2414506B1 (en) 2009-03-31 2017-05-03 Codexis, Inc. Improved endoglucanases
BRPI1011036A8 (en) 2009-05-19 2017-11-07 Danisco USE
JP5847076B2 (en) 2009-05-20 2016-01-20 バイオサイト インコーポレイテッド DNA glycosylase / lyase and AP endonuclease substrate
JP2012527247A (en) 2009-05-21 2012-11-08 ヴェレニウム コーポレイション Phytase, nucleic acid encoding phytase and method for producing and using the same
US9057097B2 (en) 2009-06-05 2015-06-16 Alere San Diego Inc. Recombinase polymerase amplification reagents and kits
AR077042A1 (en) 2009-06-12 2011-07-27 Danisco METHOD TO TREAT A COMPOSITION CONTAINING PYROPHIOPHYTIN
US8772010B2 (en) 2009-06-16 2014-07-08 Codexis, Inc. β-Glucosidase variants
EP2446026B1 (en) 2009-06-22 2017-08-09 Codexis, Inc. Transaminase reactions
WO2011005527A2 (en) 2009-06-22 2011-01-13 Codexis, Inc. Ketoreductase-mediated stereoselective route to alpha chloroalcohols
WO2011005761A1 (en) 2009-07-06 2011-01-13 Ontorii, Inc Novel nucleic acid prodrugs and methods use thereof
WO2011011630A2 (en) 2009-07-23 2011-01-27 Codexis, Inc. Nitrilase biocatalysts
US20110035843A1 (en) 2009-08-05 2011-02-10 Pioneer Hi-Bred International, Inc. Novel eto1 genes and use of same for reduced ethylene and improved stress tolerance in plants
EP2467473B1 (en) 2009-08-19 2016-03-23 Codexis, Inc. Ketoreductase polypeptides for the preparation of phenylephrine
WO2011022597A1 (en) 2009-08-20 2011-02-24 Pioneer Hi-Bred International, Inc. Functional expression of shuffled yeast nitrate transporter (ynti) in maize to improve nitrate uptake under low nitrate environment
EP2473604B1 (en) 2009-09-04 2017-01-11 Codexis, Inc. Variant cbh2 cellulases and related polynucleotides
WO2011041594A1 (en) 2009-09-30 2011-04-07 Codexis, Inc. Recombinant c1 b-glucosidase for production of sugars from cellulosic biomass
UA111708C2 (en) 2009-10-16 2016-06-10 Бандж Ойлз, Інк. METHOD OF OIL REFINING
UA109884C2 (en) 2009-10-16 2015-10-26 A POLYPEPTIDE THAT HAS THE ACTIVITY OF THE PHOSPHATIDYLINOSYTOL-SPECIFIC PHOSPHOLIPASE C, NUCLEIC ACID, AND METHOD OF METHOD
WO2011056872A2 (en) 2009-11-03 2011-05-12 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
GB0920089D0 (en) 2009-11-17 2009-12-30 Danisco Method
WO2011063080A2 (en) 2009-11-20 2011-05-26 Codexis, Inc. Mutli-cellulase enzyme compositions for hydrolysis of cellulosic biomass
PL2504017T3 (en) * 2009-11-25 2014-06-30 Codexis Inc Recombinant beta-glucosidase variants for production of soluble sugars from cellulosic biomass
WO2011066185A1 (en) 2009-11-25 2011-06-03 Gen9, Inc. Microfluidic devices and methods for gene synthesis
US8895271B2 (en) 2009-12-08 2014-11-25 Codexis, Inc. Synthesis of prazole compounds
US9217144B2 (en) 2010-01-07 2015-12-22 Gen9, Inc. Assembly of high fidelity polynucleotides
US9080192B2 (en) 2010-02-10 2015-07-14 Codexis, Inc. Processes using amino acid dehydrogenases and ketoreductase-based cofactor regenerating system
DK2545150T3 (en) 2010-03-12 2018-11-19 Dupont Nutrition Biosci Aps COURSE OF ACTION
WO2011114251A1 (en) 2010-03-18 2011-09-22 Danisco A/S Foodstuff
WO2011123576A2 (en) 2010-03-31 2011-10-06 Codexis, Inc. Production of monoterpenes
WO2011140219A1 (en) 2010-05-04 2011-11-10 Codexis, Inc. Biocatalysts for ezetimibe synthesis
WO2011143632A2 (en) 2010-05-14 2011-11-17 Codexis, Inc. Cellobiohydrolase variants
WO2011150313A1 (en) 2010-05-28 2011-12-01 Codexis, Inc. Pentose fermentation by a recombinant microorganism
CA2802017A1 (en) 2010-06-09 2011-12-15 Zymogenetics, Inc. Dimeric vstm3 fusion proteins and related compositions and methods
AR081950A1 (en) 2010-06-17 2012-10-31 Danisco SEED TREATMENT PROCESS CONTAINING OIL
NZ706052A (en) 2010-07-12 2016-07-29 Celgene Corp Romidepsin solid forms and uses thereof
WO2012021797A1 (en) 2010-08-13 2012-02-16 Pioneer Hi-Bred International, Inc. Methods and compositions for targeting sequences of interest to the chloroplast
WO2012024104A2 (en) 2010-08-16 2012-02-23 Codexis, Inc. Biocatalysts and methods for the synthesis of (1r,2r)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine
US9322027B2 (en) 2010-08-20 2016-04-26 Shell Oil Company Expression constructs comprising fungal promoters
CA2807702C (en) 2010-08-20 2018-07-24 Codexis, Inc. Use of glycoside hydrolase 61 family proteins in processing of cellulose
US8859502B2 (en) 2010-09-13 2014-10-14 Celgene Corporation Therapy for MLL-rearranged leukemia
WO2012039448A1 (en) 2010-09-24 2012-03-29 株式会社キラルジェン Asymmetric auxiliary group
CA2811787A1 (en) 2010-10-06 2012-04-19 Bp Corporation North America Inc. Variant cbh i polypeptides
BR112013010812A2 (en) 2010-11-02 2016-07-12 Codexis Inc compositions and methods for producing fermentable sugars.
US9068235B2 (en) 2010-11-02 2015-06-30 Codexis, Inc. Fungal strains
US9295965B2 (en) 2010-11-12 2016-03-29 Gen9, Inc. Methods and devices for nucleic acid synthesis
US10457935B2 (en) 2010-11-12 2019-10-29 Gen9, Inc. Protein arrays and methods of using and making the same
US9267159B2 (en) 2010-12-08 2016-02-23 Codexis, Inc. Biocatalysts and methods for the synthesis of armodafinil
BR112013015829A2 (en) 2010-12-21 2018-05-29 Codexis Inc endoglucanase variants
WO2012106579A1 (en) 2011-02-03 2012-08-09 Bristol-Myers Squibb Company Amino acid dehydrogenase and its use in preparing amino acids from keto acids
CN103797114A (en) 2011-02-23 2014-05-14 杜邦营养生物科学有限公司 Method for producing recombinant enzymes capable of hydrolysing chlorophyll a chlorophyll derivative
EP2678438B1 (en) 2011-02-23 2017-07-05 DuPont Nutrition Biosciences ApS Enzymatic treatment of chlorophyll in plant oils
AR085251A1 (en) 2011-02-23 2013-09-18 Danisco PROCESS TO TREAT VEGETABLE OIL
EP2691517A4 (en) 2011-03-30 2015-04-22 Codexis Inc Pentose fermentation by a recombinant microorganism
EP3978620A1 (en) 2011-04-07 2022-04-06 Abbott Diagnostics Scarborough, Inc. Monitoring recombinase polymerase amplification mixtures
WO2012137147A1 (en) 2011-04-08 2012-10-11 Danisco Us, Inc. Compositions
US9102963B2 (en) 2011-04-13 2015-08-11 Codexis, Inc. Biocatalytic process for preparing eslicarbazepine and analogs thereof
BR112013033614A2 (en) 2011-06-30 2018-05-29 Codexis Inc pentose fermentation by recombinant microorganism
RU2014105311A (en) 2011-07-19 2015-08-27 Уэйв Лайф Сайенсес Пте. Лтд. METHODS FOR SYNTHESIS OF FUNCTIONALIZED NUCLEIC ACIDS
US9322007B2 (en) 2011-07-22 2016-04-26 The California Institute Of Technology Stable fungal Cel6 enzyme variants
EP2748317B1 (en) 2011-08-22 2017-04-19 Codexis, Inc. Gh61 glycoside hydrolase protein variants and cofactors that enhance gh61 activity
EP2748187A4 (en) 2011-08-23 2015-03-04 Codexis Inc Cellobiohydrolase variants
AU2012300401B2 (en) 2011-08-26 2018-02-08 Gen9, Inc. Compositions and methods for high fidelity assembly of nucleic acids
WO2013036861A1 (en) 2011-09-08 2013-03-14 Codexis, Inc Biocatalysts and methods for the synthesis of substituted lactams
US20130084608A1 (en) 2011-09-30 2013-04-04 Codexis, Inc. Fungal proteases
WO2013063006A1 (en) 2011-10-25 2013-05-02 Pioneer Hi-Bred International, Inc. Methods to alter plant cell wall composition for improved biofuel production and silage digestibility
MX358862B (en) 2011-11-04 2018-09-06 Zymeworks Inc Stable heterodimeric antibody design with mutations in the fc domain.
EP2780448B1 (en) 2011-11-18 2019-09-11 Codexis, Inc. Biocatalysts for the preparation of hydroxy substituted carbamates
BR112014015030A2 (en) 2011-12-20 2017-06-13 Codexis Inc endoflucanase variants 1b (eg1b)
WO2013096082A1 (en) 2011-12-20 2013-06-27 Codexis, Inc. Fatty alcohol forming acyl reductase (far) variants and methods of use
WO2013104659A2 (en) 2012-01-13 2013-07-18 Dupont Nutrition Biosciences Aps Process
WO2013104660A1 (en) 2012-01-13 2013-07-18 Dupont Nutrition Biosciences Aps Process for treating a plant oil comprising hydrolysing chlorophyll or a chlorophyll derivative and involving partial caustic neutralisation
WO2013138339A1 (en) * 2012-03-15 2013-09-19 Codexis, Inc. Gene shuffling methods
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
CN104508126B (en) 2012-03-23 2017-06-30 科德克希思公司 Biocatalyst and method for synthesizing the derivative of tryptamines and tryptamine analogues
US20150133698A1 (en) 2012-04-20 2015-05-14 Codexis, Inc. Production of fatty alcohols from engineered microorganisms
CN111876409A (en) 2012-04-24 2020-11-03 Gen9股份有限公司 Method for sorting nucleic acids and multiplex preparations in vitro cloning
WO2013160374A1 (en) 2012-04-27 2013-10-31 Dupont Nutrition Biosciences Aps Process for refining crude plant oil involving enzymatic hydrolysis and gum recycling
WO2013160372A1 (en) 2012-04-27 2013-10-31 Dupont Nutrition Biosciences Aps Process for treating plant oil involving addition of serial doses of chlorophyll or chlorophyll derivative degrading enzyme
WO2013166113A1 (en) 2012-05-04 2013-11-07 E. I. Du Pont De Nemours And Company Compositions and methods comprising sequences having meganuclease activity
CN104428412B (en) 2012-05-08 2017-09-08 科德克希思公司 Hydroxylated biocatalyst and method for compound
CN107904216B (en) 2012-05-11 2021-06-29 科德克希思公司 Engineered imine reductases and methods for reductive amination of ketone and amine compounds
US10045499B2 (en) 2012-05-24 2018-08-14 Iowa State University Research Foundation, Inc. Arabidopsis nonhost resistance gene(s) and use thereof to engineer disease resistant plants
US8956844B2 (en) 2012-06-11 2015-02-17 Codexis, Inc. Fungal xylanases and xylosidases
CN104685116A (en) 2012-06-25 2015-06-03 Gen9股份有限公司 Methods for nucleic acid assembly and high throughput sequencing
RU2015104762A (en) 2012-07-13 2018-08-31 Уэйв Лайф Сайенсес Лтд. CHIRAL CONTROL
CN104684893B (en) 2012-07-13 2016-10-26 日本波涛生命科学公司 Asymmetric auxiliary group
MX356830B (en) 2012-07-13 2018-06-15 Shin Nippon Biomedical Laboratories Ltd Chiral nucleic acid adjuvant.
US9650655B2 (en) 2012-07-20 2017-05-16 Codexis, Inc. Production of fatty alcohols from engineered microorganisms
US9506087B2 (en) 2012-08-07 2016-11-29 Codexis, Inc. Glucose and xylose co-utilization in E. coli
ES2693461T3 (en) 2012-08-16 2018-12-11 Bangladesh Jute Research Institute Enzymes that degrade cellulose and / or hemicelluloses of Macrophomina phaseolina and uses thereof
IN2015DN02049A (en) 2012-08-16 2015-08-14 Bangladesh Jute Res Inst
AU2013202506B2 (en) 2012-09-07 2015-06-18 Celgene Corporation Resistance biomarkers for hdac inhibitors
WO2014063097A1 (en) 2012-10-19 2014-04-24 Danisco Us Inc. Stabilization of biomimetic membranes
WO2014070673A1 (en) 2012-10-29 2014-05-08 University Of Rochester Artemisinin derivatives, methods for their preparation and their use as antimalarial agents
AU2013202507B9 (en) 2012-11-14 2015-08-13 Celgene Corporation Inhibition of drug resistant cancer cells
WO2014081605A1 (en) 2012-11-20 2014-05-30 Codexis, Inc. Pentose fermentation by a recombinant microorganism
US10662424B2 (en) 2012-12-06 2020-05-26 Agilent Technologies, Inc. Molecular fabrication
US9512447B2 (en) 2012-12-14 2016-12-06 Codexis, Inc. Modified native beta-ketoacyl-ACP synthases and engineered microorganisms
JP6618180B2 (en) 2012-12-21 2019-12-11 コデクシス， インコーポレイテッド Engineered biocatalysts and methods for the synthesis of chiral amines
WO2014113521A1 (en) 2013-01-18 2014-07-24 Codexis, Inc. Engineered biocatalysts useful for carbapenem synthesis
US9684771B2 (en) 2013-01-31 2017-06-20 Codexis, Inc. Methods, systems, and software for identifying bio-molecules using models of multiplicative form
DK2954323T3 (en) 2013-02-07 2020-07-20 Univ California Use of translation profiling to identify target molecules for therapeutic treatment
US9617573B2 (en) 2013-02-28 2017-04-11 Codexis, Inc. Engineered transaminase polypeptides for industrial biocatalysis
GB201308828D0 (en) 2013-03-12 2013-07-03 Verenium Corp Phytase
CN105189527A (en) 2013-03-14 2015-12-23 科德克希思公司 Low-phosphate repressible promoter
EP2970935A1 (en) 2013-03-14 2016-01-20 Pioneer Hi-Bred International, Inc. Compositions having dicamba decarboxylase activity and methods of use
GB201308843D0 (en) 2013-03-14 2013-07-03 Verenium Corp Phytase formulation
CA2905595A1 (en) 2013-03-14 2014-09-25 Pioneer Hi-Bred International, Inc. Compositions having dicamba decarboxylase activity and methods of use
CN105247056A (en) 2013-03-15 2016-01-13 先锋国际良种公司 Modulation of acc deaminase expression
CA2901316A1 (en) 2013-03-15 2014-09-25 Pioneer Hi-Bred International, Inc. Phi-4 polypeptides and methods for their use
WO2014151696A1 (en) * 2013-03-15 2014-09-25 Gen9, Inc. Compositions and methods for multiplex nucleic acids synthesis
PE20151775A1 (en) 2013-04-18 2015-12-02 Codexis Inc POLYPEPTIDES OF PHENYLALANINE AMMONIA LIASA MODIFIED BY GENETIC ENGINEERING
WO2014202089A2 (en) 2013-06-18 2014-12-24 Roskilde Universitet Variants of anti-freeze polypeptides
US10093909B2 (en) 2013-07-25 2018-10-09 Basf Enzymes Llc Phytase
CN106232820A (en) 2013-08-16 2016-12-14 先锋国际良种公司 Insecticidal protein and using method thereof
BR122020001770B1 (en) 2013-09-13 2022-11-29 Pioneer Hi-Bred International, Inc DNA CONSTRUCTION, METHOD FOR OBTAINING A TRANSGENIC PLANT, FUSION PROTEIN, METHOD FOR CONTROLLING AN INSECT PEST POPULATION, METHOD FOR INHIBITING THE GROWTH OR KILLING AN INSECT PEST
US10696964B2 (en) 2013-09-27 2020-06-30 Codexis, Inc. Automated screening of enzyme variants
EP3068793B1 (en) 2013-11-13 2021-02-17 Codexis, Inc. Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
WO2015094527A1 (en) 2013-12-19 2015-06-25 Danisco Us Inc. Use of hydrophobins to increase gas transferin aerobic fermentation processes
US10544191B2 (en) 2013-12-23 2020-01-28 University Of Rochester Methods and compositions for ribosomal synthesis of macrocyclic peptides
NZ630311A (en) 2013-12-27 2016-03-31 Celgene Corp Romidepsin formulations and uses thereof
EP3095459A4 (en) 2014-01-15 2017-08-23 Shin Nippon Biomedical Laboratories, Ltd. Chiral nucleic acid adjuvant having antitumor effect and antitumor agent
US10322173B2 (en) 2014-01-15 2019-06-18 Shin Nippon Biomedical Laboratories, Ltd. Chiral nucleic acid adjuvant having anti-allergic activity, and anti-allergic agent
US10144933B2 (en) 2014-01-15 2018-12-04 Shin Nippon Biomedical Laboratories, Ltd. Chiral nucleic acid adjuvant having immunity induction activity, and immunity induction activator
ES2917473T3 (en) 2014-01-16 2022-07-08 Wave Life Sciences Ltd chiral design
UA120608C2 (en) 2014-02-07 2020-01-10 Піонір Хай-Бред Інтернешнл, Інк. Insecticidal proteins and methods for their use
CA2938979C (en) 2014-02-07 2024-02-20 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
US20170121741A1 (en) 2014-04-01 2017-05-04 Dupont Nutrition Biosciences Aps Method for increasing crude palm oil yields
EP3145536B1 (en) 2014-04-16 2021-10-27 Codexis, Inc. Engineered tyrosine ammonia lyase
WO2016007623A1 (en) 2014-07-09 2016-01-14 Codexis, Inc. Novel p450-bm3 variants with improved activity
EP3450558B1 (en) 2014-08-27 2020-09-30 New England Biolabs, Inc. Synthon formation
US9963687B2 (en) 2014-08-27 2018-05-08 New England Biolabs, Inc. Fusion polymerase and method for using the same
WO2016033304A1 (en) 2014-08-28 2016-03-03 Codexis, Inc. Imidazoyl anilide derivatives and methods of use
US10273229B2 (en) 2014-08-28 2019-04-30 Codexis, Inc. N-substituted 4-aminoquinazoline derivatives and methods of use
CN113372421A (en) 2014-10-16 2021-09-10 先锋国际良种公司 Insecticidal proteins and methods of use thereof
WO2016085916A1 (en) 2014-11-25 2016-06-02 Codexis, Inc. Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
WO2016183532A1 (en) 2015-05-13 2016-11-17 Synlogic, Inc. Bacteria engineered to treat a disease or disorder
PL3237621T3 (en) 2014-12-22 2023-10-30 Codexis, Inc. Human alpha-galactosidase variants
ES2830725T3 (en) 2015-02-10 2021-06-04 Codexis Inc Ketoreductase polypeptides for the synthesis of chiral compounds
CA2977026A1 (en) 2015-03-11 2016-09-15 E.I. Du Pont De Nemours And Company Insecticidal combinations of pip-72 and methods of use
EP3280817A2 (en) 2015-04-07 2018-02-14 Novozymes A/S Methods for selecting enzymes having enhanced activity
CN107864658A (en) 2015-04-07 2018-03-30 诺维信公司 Method for selecting the enzyme with lipase active
MX2017014213A (en) 2015-05-07 2018-03-28 Codexis Inc Penicillin-g acylases.
US9920102B2 (en) 2015-05-15 2018-03-20 Albert Einstein College Of Medicine, Inc. Fusion tags for protein expression
CN108064233B (en) 2015-05-19 2022-07-15 先锋国际良种公司 Insecticidal proteins and methods of use thereof
US10526597B2 (en) * 2015-05-21 2020-01-07 Full Spectrum Genetics, Inc. Method of improving characteristics of proteins
WO2016210384A2 (en) 2015-06-25 2016-12-29 Synlogic, Inc. Bacteria engineered to treat metabolic diseases
DK3319987T3 (en) 2015-07-07 2021-06-21 Codexis Inc NEW P450-BM3 VARIANTS WITH IMPROVED ACTIVITY
WO2017023486A1 (en) 2015-08-06 2017-02-09 Pioneer Hi-Bred International, Inc. Plant derived insecticidal proteins and methods for their use
EP3328881B1 (en) 2015-09-08 2019-08-28 Theripion, Inc. Apoa-1 fusion polypeptides and related compositions and methods
GB201516348D0 (en) * 2015-09-15 2015-10-28 Labgenius Ltd Compositions and methods for polynucleotide assembly
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
CA3007635A1 (en) 2015-12-07 2017-06-15 Zymergen Inc. Promoters from corynebacterium glutamicum
WO2017105987A1 (en) 2015-12-18 2017-06-22 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
WO2017123592A1 (en) 2016-01-11 2017-07-20 Synlogic, Inc. Bacteria engineered to treat disorders associated with bile salts
WO2017123676A1 (en) 2016-01-11 2017-07-20 Synlogic, Inc. Recombinant bacteria engineered to treat diseases and disorders associated with amino acid metabolism and methods of use thereof
WO2017123418A1 (en) 2016-01-11 2017-07-20 Synlogic, Inc. Bacteria engineered to treat metabolic diseases
WO2017139708A1 (en) 2016-02-10 2017-08-17 Synlogic, Inc. Bacteria engineered to treat nonalcoholic steatohepatitis (nash)
EP3440207A1 (en) 2016-04-07 2019-02-13 Novozymes A/S Methods for selecting enzymes having protease activity
BR112018072417B1 (en) 2016-05-04 2023-03-14 E. I. Du Pont De Nemours And Company RECOMBINANT INSECTICIDAL POLYPEPTIDE, CHIMERIC POLYPEPTIDE, COMPOSITION, RECOMBINANT POLYNUCLEOTIDE, DNA CONSTRUCTS, METHODS FOR OBTAINING A TRANSGENIC PLANT, METHODS FOR INHIBITING THE GROWTH OR EXTERMINATION OF AN INSECT PEST OR PEST POPULATION, METHOD FOR OBTAINING A TRANSFORMED PROKARYOTIC CELL TRANSFORMED AND METHOD TO GENETICALLY MODIFY THE INSECTICIDAL POLYPEPTIDE
CN109715793A (en) 2016-05-05 2019-05-03 科德克希思公司 Penicillin-G acylase
EP3469088B1 (en) 2016-06-09 2023-09-06 Codexis, Inc. Biocatalysts and methods for hydroxylation of chemical compounds
US11299723B2 (en) 2016-06-15 2022-04-12 Codexis, Inc. Engineered beta-glucosidases and glucosylation methods
US20190330641A1 (en) 2016-06-17 2019-10-31 National Hellenic Research Foundation Systems for recombinant protein production
WO2018005655A2 (en) 2016-06-30 2018-01-04 Zymergen Inc. Methods for generating a bacterial hemoglobin library and uses thereof
EP3478845A4 (en) 2016-06-30 2019-07-31 Zymergen, Inc. Methods for generating a glucose permease library and uses thereof
RU2019102714A (en) 2016-07-01 2020-08-03 Пайонир Хай-Бред Интернэшнл, Инк. INSECTICIDE PROTEINS FROM PLANTS AND METHODS OF THEIR APPLICATION
SG10202111589RA (en) 2016-08-26 2021-11-29 Codexis Inc Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
EP3535285B1 (en) 2016-11-01 2022-04-06 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
WO2018136163A2 (en) 2016-12-09 2018-07-26 Theripion, Inc. Tandem apoa-1 fusion polypeptides
CN110088123B (en) 2016-12-14 2023-10-20 先锋国际良种公司 Insecticidal proteins and methods of use thereof
WO2018118811A1 (en) 2016-12-22 2018-06-28 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
WO2018129130A1 (en) 2017-01-05 2018-07-12 Codexis, Inc. Penicillin-g acylases
EP3577229A4 (en) 2017-02-03 2020-12-23 Codexis, Inc. Engineered glycosyltransferases and steviol glycoside glucosylation methods
US20190390219A1 (en) 2017-02-08 2019-12-26 Pioneer Hi-Bred International, Inc. Insecticidal combinations of plant derived insecticidal proteins and methods for their use
SG11201906880VA (en) * 2017-02-13 2019-08-27 Codexis Inc Engineered phenylalanine ammonia lyase polypeptides
WO2018200214A2 (en) 2017-04-27 2018-11-01 Codexis, Inc. Ketoreductase polypeptides and polynucleotides
EP3635101B1 (en) 2017-05-08 2021-08-18 Codexis, Inc. Engineered ligase variants
US11555203B2 (en) 2017-05-11 2023-01-17 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
WO2018209301A1 (en) 2017-05-11 2018-11-15 Cytodyn Inc. Methods for treating or preventing graft-versus-host disease involving the administration of anti-ccr5 receptor agents
CA3064612A1 (en) * 2017-06-06 2018-12-13 Zymergen Inc. A htp genomic engineering platform for improving escherichia coli
WO2018226893A2 (en) * 2017-06-06 2018-12-13 Zymergen Inc. A high-throughput (htp) genomic engineering platform for improving saccharopolyspora spinosa
US11359183B2 (en) 2017-06-14 2022-06-14 Codexis, Inc. Engineered transaminase polypeptides for industrial biocatalysis
CN111051506B (en) 2017-06-27 2023-11-24 科德克希思公司 Penicillin G acylase
EP3645712A4 (en) 2017-06-30 2021-07-07 Codexis, Inc. T7 rna polymerase variants
US10793841B2 (en) 2017-06-30 2020-10-06 Codexis, Inc. T7 RNA polymerase variants
US11634400B2 (en) 2017-08-19 2023-04-25 University Of Rochester Micheliolide derivatives, methods for their preparation and their use as anticancer and antiinflammatory agents
EP3706780A4 (en) 2017-11-07 2021-12-22 Codexis, Inc. Transglutaminase variants
SG11202004185PA (en) 2017-12-13 2020-06-29 Codexis Inc Carboxyesterase polypeptides for amide coupling
US11708571B2 (en) 2018-05-17 2023-07-25 Bp Corporation North America Inc. Production of 2-keto-3-deoxy-d-gluconic acid in filamentous fungi
CA3102968A1 (en) 2018-06-12 2019-12-19 Codexis, Inc. Engineered tyrosine ammonia lyase
EP3821000A4 (en) 2018-07-09 2022-07-20 Codexis, Inc. Engineered galactose oxidase variant enzymes
AU2019302422B2 (en) 2018-07-09 2022-08-04 Codexis, Inc. Engineered phosphopentomutase variant enzymes
AU2019300836A1 (en) 2018-07-09 2021-01-07 Codexis, Inc. Engineered deoxyribose-phosphate aldolases
JP7461658B2 (en) 2018-07-09 2024-04-04 コデクシス， インコーポレイテッド Engineered Purine Nucleoside Phosphorylase Variant Enzymes
WO2020014050A1 (en) 2018-07-09 2020-01-16 Codexis, Inc. Engineered pantothenate kinase variant enzymes
US11198861B2 (en) 2018-07-12 2021-12-14 Codexis, Inc. Engineered phenylalanine ammonia lyase polypeptides
CN112805295A (en) 2018-07-30 2021-05-14 科德克希思公司 Engineering glycosyltransferases and methods of glycosylation of steviol glycosides
WO2020049155A1 (en) 2018-09-07 2020-03-12 Basf Plant Science Company Gmbh Improved method for the production of high levels of pufa in plants
US20210317467A1 (en) 2018-09-07 2021-10-14 Basf Plant Science Copmany Gmbh Improved method for the production of high levels of pufa in plants
CN112996915A (en) 2018-09-07 2021-06-18 巴斯夫植物科学有限公司 Improved methods for producing high levels of PUFAs in plants
US11060075B2 (en) 2018-10-29 2021-07-13 Codexis, Inc. Engineered DNA polymerase variants
US11473077B2 (en) 2018-12-14 2022-10-18 Codexis, Inc. Engineered tyrosine ammonia lyase
JP2022515742A (en) 2018-12-20 2022-02-22 コデクシス， インコーポレイテッド Alpha-galactosidase variant
JP2022524215A (en) 2019-03-28 2022-04-28 ダニスコ・ユーエス・インク Modified antibody
MX2022001411A (en) 2019-08-02 2022-03-25 Cytodyn Inc Methods for treating or preventing cancers involving the administration of anti-ccr5 receptor agents.
BR112022011895A2 (en) 2019-12-20 2022-09-06 Basf Se DECREASE IN TERPENE TOXIDITY AND INCREASED POTENTIAL PRODUCTION IN MICROORGANISMS
US11045546B1 (en) 2020-03-30 2021-06-29 Cytodyn Inc. Methods of treating coronavirus infection
US11788071B2 (en) 2020-04-10 2023-10-17 Codexis, Inc. Engineered transaminase polypeptides
US20230235310A1 (en) 2020-06-04 2023-07-27 Isobionics B.V. Synthetic santalene synthases
BR112022027035A2 (en) 2020-07-14 2023-04-11 Pioneer Hi Bred Int INSECTICIDAL PROTEINS AND METHODS FOR THE USE OF THEM
JP2023539634A (en) 2020-08-28 2023-09-15 コデクシス， インコーポレイテッド Engineered amylase variants
EP4259772A1 (en) 2020-12-11 2023-10-18 Willow Biosciences, Inc. Recombinant acyl activating enzyme (aae) genes for enhanced biosynthesis of cannabinoids and cannabinoid precursors
US11898174B2 (en) 2020-12-18 2024-02-13 Codexis, Inc. Engineered uridine phosphorylase variant enzymes
EP4015626A1 (en) 2020-12-18 2022-06-22 Isobionics B.V. Enzymes and methods for fermentative production of monoterpene esters
WO2022178078A1 (en) 2021-02-19 2022-08-25 Theripion, Inc. Paraoxonase fusion polypeptides and related compositions and methods
US20220325285A1 (en) 2021-04-02 2022-10-13 Codexis, Inc. ENGINEERED CYCLIC GMP-AMP SYNTHASE (cGAS) VARIANT ENZYMES
CN117222735A (en) 2021-04-02 2023-12-12 科德克希思公司 Engineered acetate kinase variant enzymes
US20220325284A1 (en) 2021-04-02 2022-10-13 Codexis, Inc. Engineered guanylate kinase variant enzymes
IL305919A (en) 2021-04-02 2023-11-01 Codexis Inc Engineered adenylate kinase variant enzymes
WO2023010083A2 (en) 2021-07-30 2023-02-02 Willow Biosciences, Inc. Recombinant prenyltransferase polypeptides engineered for enhanced biosynthesis of cannabinoids
CN117795087A (en) 2021-08-02 2024-03-29 巴斯夫欧洲公司 Novel production of aromatic compounds with violet subunit ethane synthase
CA3227236A1 (en) 2021-08-19 2023-02-23 Trish Choudhary Recombinant olivetolic acid cyclase polypeptides engineered for enhanced biosynthesis of cannabinoids
WO2023069921A1 (en) 2021-10-19 2023-04-27 Epimeron Usa, Inc. Recombinant thca synthase polypeptides engineered for enhanced biosynthesis of cannabinoids
CN114015672B (en) * 2021-12-06 2022-05-31 江南大学 Pfu DNA polymerase
WO2023173084A1 (en) 2022-03-11 2023-09-14 University Of Rochester Cyclopeptibodies and uses thereof
WO2023245171A1 (en) 2022-06-17 2023-12-21 Synlogic Operating Company, Inc. Bacteria engineered to treat diseases associated with bile acid metabolism and methods of use thereof
WO2023245168A1 (en) 2022-06-17 2023-12-21 Synlogic Operating Company, Inc. Bacteria engineered to treat diseases associated with bile acid metabolism and methods of use thereof
WO2023250478A1 (en) 2022-06-23 2023-12-28 Synlogic Operating Company, Inc. Recombinant bacteria engineered to treat diseases associated with methionine metabolism and methods of use thereof
WO2024040020A1 (en) 2022-08-15 2024-02-22 Absci Corporation Quantitative affinity activity specific cell enrichment

Family Cites Families (119)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4994379A (en) 1983-03-09 1991-02-19 Cetus Corporation Modified signal peptides
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
US5422266A (en) * 1984-12-31 1995-06-06 University Of Georgia Research Foundation, Inc. Recombinant DNA vectors capable of expressing apoaequorin
US5176995A (en) * 1985-03-28 1993-01-05 Hoffmann-La Roche Inc. Detection of viruses by amplification and hybridization
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
EP0590689B2 (en) * 1985-03-30 2006-08-16 KAUFFMAN, Stuart A. Method for obtaining DNA, RNA, peptides, polypeptides or proteins by means of a DNA-recombinant technique
US4959312A (en) * 1985-05-31 1990-09-25 The University Of Tennessee Research Corporation Full spectrum mutagenesis
EP0208491B1 (en) * 1985-07-03 1993-08-25 Genencor International, Inc. Hybrid polypeptides and process for their preparation
US5866363A (en) 1985-08-28 1999-02-02 Pieczenik; George Method and means for sorting and identifying biological information
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
DK311186D0 (en) * 1986-06-30 1986-06-30 Novo Industri As ENZYMES
US5824469A (en) 1986-07-17 1998-10-20 University Of Washington Method for producing novel DNA sequences with biological activity
US5763192A (en) * 1986-11-20 1998-06-09 Ixsys, Incorporated Process for obtaining DNA, RNA, peptides, polypeptides, or protein, by recombinant DNA technique
US4994368A (en) * 1987-07-23 1991-02-19 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
FR2641793B1 (en) 1988-12-26 1993-10-01 Setratech METHOD OF IN VIVO RECOMBINATION OF DNA SEQUENCES HAVING BASIC MATCHING
DD282028A5 (en) 1989-02-16 1990-08-29 Akad Wissenschaften Ddr PROCESS FOR PREPARING THERMOSTABILES, HYBRIDEN BACILLUS BETA-1,3-1,4-GLUCANASE
US5043272A (en) * 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
US5106727A (en) 1989-04-27 1992-04-21 Life Technologies, Inc. Amplification of nucleic acid sequences using oligonucleotides of random sequences as primers
US5556750A (en) * 1989-05-12 1996-09-17 Duke University Methods and kits for fractionating a population of DNA molecules based on the presence or absence of a base-pair mismatch utilizing mismatch repair systems
CA2016841C (en) 1989-05-16 1999-09-21 William D. Huse A method for producing polymers having a preselected activity
CA2016842A1 (en) * 1989-05-16 1990-11-16 Richard A. Lerner Method for tapping the immunological repertoire
IT1231157B (en) * 1989-07-20 1991-11-19 Crc Ricerca Chim NEW FUNCTIONAL HYBRID GENES OF BACILLUS THURINGIENSIS OBTAINED THROUGH IN VIVO RECOMBINATION.
US5574205A (en) * 1989-07-25 1996-11-12 Cell Genesys Homologous recombination for universal donor cells and chimeric mammalian hosts
US5023171A (en) * 1989-08-10 1991-06-11 Mayo Foundation For Medical Education And Research Method for gene splicing by overlap extension using the polymerase chain reaction
WO1991006570A1 (en) * 1989-10-25 1991-05-16 The University Of Melbourne HYBRID Fc RECEPTOR MOLECULES
US5071743A (en) * 1989-10-27 1991-12-10 Her Majesty The Queen In Right Of Canada, As Represented By The National Research Council Of Canada Process for conducting site-directed mutagenesis
DE3936258C1 (en) * 1989-10-31 1991-04-25 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften Ev, 3400 Goettingen, De
WO1991007506A1 (en) * 1989-11-08 1991-05-30 The United States Of America, Represented By The Secretary, United States Department Of Commerce A method of synthesizing double-stranded dna molecules
US5242937A (en) 1990-03-19 1993-09-07 Research Corporation Technologies, Inc. Topically active ocular thiadiazole sulfonamide carbonic anhydrase inhibitors
EP0527809B1 (en) * 1990-04-05 1995-08-16 CREA, Roberto Walk-through mutagenesis
AU7791991A (en) * 1990-04-24 1991-11-11 Stratagene Methods for phenotype creation from multiple gene populations
US5877402A (en) 1990-05-01 1999-03-02 Rutgers, The State University Of New Jersey DNA constructs and methods for stably transforming plastids of multicellular plants and expressing recombinant proteins therein
US5851813A (en) * 1990-07-12 1998-12-22 President And Fellows Of Harvard College Primate lentivirus antigenic compositions
JP3392863B2 (en) * 1990-07-24 2003-03-31 エフ．ホフマン ― ラ ロシュ アーゲー Reduction of non-specific amplification in in vitro nucleic acid amplification using modified nucleobases
DE69130647T2 (en) 1990-08-24 1999-05-06 Ixsys Inc METHOD FOR PRODUCING OLIGONUCLEOTIDES WITH REGULAR CODONES
US5264563A (en) 1990-08-24 1993-11-23 Ixsys Inc. Process for synthesizing oligonucleotides with random codons
US5770434A (en) 1990-09-28 1998-06-23 Ixsys Incorporated Soluble peptides having constrained, secondary conformation in solution and method of making same
US5698426A (en) 1990-09-28 1997-12-16 Ixsys, Incorporated Surface expression libraries of heteromeric receptors
US5871974A (en) 1990-09-28 1999-02-16 Ixsys Inc. Surface expression libraries of heteromeric receptors
IL99553A0 (en) 1990-09-28 1992-08-18 Ixsys Inc Compositions containing oligonucleotides linked to expression elements,a kit for the preparation of vectors useful for the expression of a diverse population of random peptides and methods utilizing the same
US5858725A (en) * 1990-10-10 1999-01-12 Glaxo Wellcome Inc. Preparation of chimaeric antibodies using the recombinant PCR strategy
US5187083A (en) * 1990-11-13 1993-02-16 Specialty Laboratories, Inc. Rapid purification of DNA
US5234824A (en) * 1990-11-13 1993-08-10 Specialty Laboratories, Inc. Rapid purification of DNA
US5489523A (en) * 1990-12-03 1996-02-06 Stratagene Exonuclease-deficient thermostable Pyrococcus furiosus DNA polymerase I
WO1992011272A1 (en) 1990-12-20 1992-07-09 Ixsys, Inc. Optimization of binding proteins
US5098437A (en) 1991-02-13 1992-03-24 Pfizer Hospital Products Group, Inc. Acetabular cup positioning insert
US5795747A (en) * 1991-04-16 1998-08-18 Evotec Biosystems Gmbh Process for manufacturing new biopolymers
DE4112440C1 (en) 1991-04-16 1992-10-22 Diagen Institut Fuer Molekularbiologische Diagnostik Gmbh, 4000 Duesseldorf, De
US5514568A (en) * 1991-04-26 1996-05-07 Eli Lilly And Company Enzymatic inverse polymerase chain reaction
US5512463A (en) * 1991-04-26 1996-04-30 Eli Lilly And Company Enzymatic inverse polymerase chain reaction library mutagenesis
EP0519338B1 (en) 1991-06-20 1996-08-28 F. Hoffmann-La Roche Ag Improved methods for nucleic acid amplification
WO1993000103A1 (en) * 1991-06-21 1993-01-07 The Wistar Institute Of Anatomy And Biology Chimeric envelope proteins for viral targeting
JP3442774B2 (en) * 1991-07-01 2003-09-02 バーレックス ラボラトリーズ，インコーポレイティド Novel mutagenesis methods and compositions
US6071889A (en) 1991-07-08 2000-06-06 Neurospheres Holdings Ltd. In vivo genetic modification of growth factor-responsive neural precursor cells
US5223408A (en) * 1991-07-11 1993-06-29 Genentech, Inc. Method for making variant secreted proteins with altered properties
GB9115364D0 (en) * 1991-07-16 1991-08-28 Wellcome Found Antibody
US5279952A (en) * 1991-08-09 1994-01-18 Board Of Regents, The University Of Texas System PCR-based strategy of constructing chimeric DNA molecules
ATE181571T1 (en) * 1991-09-23 1999-07-15 Medical Res Council METHODS FOR PRODUCING HUMANIZED ANTIBODIES
GB9125979D0 (en) * 1991-12-06 1992-02-05 Wellcome Found Antibody
EP0625205A1 (en) * 1992-01-30 1994-11-23 Genzyme Limited Chiral synthesis with modified enzymes
US5773267A (en) * 1992-02-07 1998-06-30 Albert Einstein College Of Medicine Of Yeshiva University, A Division Of Yeshiva University D29 shuttle phasmids and uses thereof
GB9202796D0 (en) * 1992-02-11 1992-03-25 Wellcome Found Antiviral antibody
US5843643A (en) * 1992-02-21 1998-12-01 Ratner; Paul L. Site-specific transfection of eukaryotic cells using polypeptide-linked recombinant nucleic acid
WO1993018141A1 (en) * 1992-03-02 1993-09-16 Biogen, Inc. Thrombin receptor antagonists
ES2231769T3 (en) 1992-03-16 2005-05-16 Jacob N. Wohlstadter MUTAGENESIS AND SELECTION PROCEDURES IN THE SAME CELLS GUEST.
US5316935A (en) * 1992-04-06 1994-05-31 California Institute Of Technology Subtilisin variants suitable for hydrolysis and synthesis in organic media
EP0606430A1 (en) * 1992-06-15 1994-07-20 City Of Hope Chimeric anti-cea antibody
CA2139876A1 (en) * 1992-07-10 1994-01-20 John Rambosek Recombinant dna encoding a desulfurization biocatalyst
US6107062A (en) * 1992-07-30 2000-08-22 Inpax, Inc. Antisense viruses and antisense-ribozyme viruses
FR2694754B1 (en) * 1992-08-12 1994-09-16 Bio Merieux Mycobacteria DNA fragments, amplification primers, hybridization probes, reagents and method for detecting detection of mycobacteria.
DE69232604T2 (en) * 1992-11-04 2002-11-07 City Of Hope Duarte ANTIBODY CONSTRUCTS
JPH08505524A (en) 1992-11-10 1996-06-18 イグジス， インコーポレイティッド Soluble peptide having a secondary conformation constrained in solution, and process for producing the same
US5360728A (en) * 1992-12-01 1994-11-01 Woods Hole Oceanographic Institution (W.H.O.I.) Modified apoaequorin having increased bioluminescent activity
DK0672142T3 (en) * 1992-12-04 2001-06-18 Medical Res Council Multivalent and multi-specific binding proteins as well as their preparation and use
US5380530A (en) 1992-12-29 1995-01-10 Whitehill Oral Technologies Oral care composition coated gum
IT1264712B1 (en) * 1993-07-13 1996-10-04 Eniricerche Spa BIOLOGICAL SYNTHESIS METHOD OF PEPTIDES
US5498531A (en) 1993-09-10 1996-03-12 President And Fellows Of Harvard College Intron-mediated recombinant techniques and reagents
US5556772A (en) * 1993-12-08 1996-09-17 Stratagene Polymerase compositions and uses thereof
DE4343591A1 (en) * 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Process for the evolutionary design and synthesis of functional polymers based on shape elements and shape codes
US5837458A (en) * 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5928905A (en) 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6165793A (en) 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5834252A (en) * 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5521077A (en) * 1994-04-28 1996-05-28 The Leland Stanford Junior University Method of generating multiple protein variants and populations of protein variants prepared thereby
US5514588A (en) 1994-12-13 1996-05-07 Exxon Research And Engineering Company Surfactant-nutrients for bioremediation of hydrocarbon contaminated soils and water
US5629179A (en) * 1995-03-17 1997-05-13 Novagen, Inc. Method and kit for making CDNA library
DE19612631A1 (en) 1995-03-31 1996-10-02 Mazda Motor Multiplex data transmitting system
HUP9801871A3 (en) * 1995-04-24 1999-12-28 Chromaxome Corp San Diego Methods for generating and screening novel metabolic pathways
US6004788A (en) 1995-07-18 1999-12-21 Diversa Corporation Enzyme kits and libraries
US6030779A (en) 1995-07-18 2000-02-29 Diversa Corporation Screening for novel bioactivities
US6168919B1 (en) 1996-07-17 2001-01-02 Diversa Corporation Screening methods for enzymes and enzyme kits
US6057103A (en) 1995-07-18 2000-05-02 Diversa Corporation Screening for novel bioactivities
US5958672A (en) * 1995-07-18 1999-09-28 Diversa Corporation Protein activity screening of clones having DNA from uncultivated microorganisms
WO1997007206A1 (en) * 1995-08-11 1997-02-27 Okkels, Jens, Sigurd Method for preparing polypeptide variants
US5962258A (en) 1995-08-23 1999-10-05 Diversa Corporation Carboxymethyl cellulase fromthermotoga maritima
US6051409A (en) * 1995-09-25 2000-04-18 Novartis Finance Corporation Method for achieving integration of exogenous DNA delivered by non-biological means to plant cells
US5756316A (en) 1995-11-02 1998-05-26 Genencor International, Inc. Molecular cloning by multimerization of plasmids
US6238884B1 (en) 1995-12-07 2001-05-29 Diversa Corporation End selection in directed evolution
US6171820B1 (en) 1995-12-07 2001-01-09 Diversa Corporation Saturation mutagenesis in directed evolution
US20030215798A1 (en) 1997-06-16 2003-11-20 Diversa Corporation High throughput fluorescence-based screening for novel enzymes
US5939250A (en) 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
US5830696A (en) 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
US5965408A (en) * 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
AU1367897A (en) * 1996-01-10 1997-08-01 Novo Nordisk A/S A method for in vivo production of a mutant library in cells
US6096548A (en) 1996-03-25 2000-08-01 Maxygen, Inc. Method for directing evolution of a virus
US5783431A (en) * 1996-04-24 1998-07-21 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
US5763239A (en) 1996-06-18 1998-06-09 Diversa Corporation Production and use of normalized DNA libraries
US6093873A (en) 1996-08-19 2000-07-25 Institut National De La Sante Et De La Recherche Medicale Genetically engineered mice containing alterations in the gene encoding RXR
WO1998028416A1 (en) * 1996-12-20 1998-07-02 Novo Nordisk A/S In vivo recombination
DK1015575T3 (en) * 1997-03-18 2010-08-23 Novozymes As Shuffling of heterologous DNA sequences
DE69840382D1 (en) * 1997-03-18 2009-02-05 Novozymes As METHOD FOR THE PRODUCTION OF A LIBRARY THROUGH DNA SHUFFLING
KR20000076363A (en) * 1997-03-18 2000-12-26 한센 핀 베네드, 안네 제헤르, 웨이콥 마리안느 An in vitro method for construction of a dna library
US5948653A (en) * 1997-03-21 1999-09-07 Pati; Sushma Sequence alterations using homologous recombination
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
US6051049A (en) 1997-05-29 2000-04-18 Exothermic Distribution Corporation Utilization of strontium aluminate in steelmaking
US6087341A (en) 1998-02-12 2000-07-11 The Board Of Trustees Of The Leland Standford Junior University Introduction of nucleic acid into skin cells by topical application

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8372636B2 (en) 1996-05-31 2013-02-12 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US9139637B2 (en) 1996-05-31 2015-09-22 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US7458125B2 (en) * 2002-09-20 2008-12-02 Colgate-Palmolive Company Oral care implement

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