CA2258489A1 - High-throughput screening assay systems in microscale fluidic devices - Google Patents

High-throughput screening assay systems in microscale fluidic devices

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Publication number
CA2258489A1
CA2258489A1 CA002258489A CA2258489A CA2258489A1 CA 2258489 A1 CA2258489 A1 CA 2258489A1 CA 002258489 A CA002258489 A CA 002258489A CA 2258489 A CA2258489 A CA 2258489A CA 2258489 A1 CA2258489 A1 CA 2258489A1
Authority
CA
Canada
Prior art keywords
channel
channels
throughput screening
screening assay
assay systems
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002258489A
Other languages
French (fr)
Other versions
CA2258489C (en
Inventor
John Wallace Parce
Ann R. Kopf-Sill
Luc J. Bousse
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Caliper Life Sciences Inc
Original Assignee
Caliper Technologies Corporation
John Wallace Parce
Ann R. Kopf-Sill
Luc J. Bousse
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/671,987 external-priority patent/US5942443A/en
Application filed by Caliper Technologies Corporation, John Wallace Parce, Ann R. Kopf-Sill, Luc J. Bousse filed Critical Caliper Technologies Corporation
Publication of CA2258489A1 publication Critical patent/CA2258489A1/en
Application granted granted Critical
Publication of CA2258489C publication Critical patent/CA2258489C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Abstract

The present invention provides microfluidic devices and methods that are useful for performing high-throughput screening assays. In particular, the devices and methods of the invention are useful in screening large numbers of different compounds for their effects on a variety of chemical, and preferably, biochemical systems. The device includes a series of channels (110, 112), and optional reagent channel (114), fabricated into the surface of the substrate. At least one of these channels will typically have very small cross-sectional dimensions, e.g.
in the range of from about 0.1 hem to about 500 µm. The device also includes reservoirs (104, 106 and 108), disposed and fluidly connected at the ends of the channels (110 and 114). As shown, sample channel (112) is used to introduce the plurality of different test compounds into the device. As such, this channel will generally be fluidly connected to a source of large numbers of separate test compounds that will be individually introduced into the sample channel (112) and subsequently into channel (110).
CA002258489A 1996-06-28 1997-06-24 High-throughput screening assay systems in microscale fluidic devices Expired - Fee Related CA2258489C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US08/671,987 US5942443A (en) 1996-06-28 1996-06-28 High throughput screening assay systems in microscale fluidic devices
US08/671,987 1996-06-28
US08/761,575 1996-12-06
US08/761,575 US6046056A (en) 1996-06-28 1996-12-06 High throughput screening assay systems in microscale fluidic devices
PCT/US1997/010894 WO1998000231A1 (en) 1996-06-28 1997-06-24 High-throughput screening assay systems in microscale fluidic devices

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CA2258489A1 true CA2258489A1 (en) 1998-01-08
CA2258489C CA2258489C (en) 2004-01-27

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EP (1) EP0907412B1 (en)
JP (1) JP3788519B2 (en)
CN (1) CN1173776C (en)
BR (1) BR9710054A (en)
CA (1) CA2258489C (en)
NZ (1) NZ333346A (en)
WO (1) WO1998000231A1 (en)

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