CA2268397A1 - Magnetically activated cell sorting for production of proteins - Google Patents

Magnetically activated cell sorting for production of proteins

Info

Publication number
CA2268397A1
CA2268397A1 CA002268397A CA2268397A CA2268397A1 CA 2268397 A1 CA2268397 A1 CA 2268397A1 CA 002268397 A CA002268397 A CA 002268397A CA 2268397 A CA2268397 A CA 2268397A CA 2268397 A1 CA2268397 A1 CA 2268397A1
Authority
CA
Canada
Prior art keywords
protein
cells
virus
antigen
proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002268397A
Other languages
French (fr)
Other versions
CA2268397C (en
Inventor
Donald L. Siegel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Pennsylvania Penn
Original Assignee
The Trustees Of The University Of Pennsylvania
Donald L. Siegel
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Trustees Of The University Of Pennsylvania, Donald L. Siegel filed Critical The Trustees Of The University Of Pennsylvania
Publication of CA2268397A1 publication Critical patent/CA2268397A1/en
Application granted granted Critical
Publication of CA2268397C publication Critical patent/CA2268397C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/34—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against blood group antigens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display

Abstract

The invention includes methods of isolating DNA and a protein encoded thereby, the protein being capable of binding to an antigen-bearing moiety. The methods comprise generating a DNA library expressing the protein in a virus vector. A magnetic label is added to cells expressing the antigen-bearing moiety and the cells are incubated with virus expressing the protein in the presence of an excess of unlabeled cells which do not express the antigen-bearing moiety to form a mixture, wherein the virus binds to the magnerically labeled cells. Cells having virus bound thereon are isolated from the mixture and DNA encoding the protein and the protein are obtained therefrom.
CA002268397A 1996-10-11 1997-10-10 Magnetically activated cell sorting for production of proteins Expired - Lifetime CA2268397C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US2855096P 1996-10-11 1996-10-11
US60/028,550 1996-10-11
US08/884,045 1997-06-27
US08/884,045 US5876925A (en) 1996-10-11 1997-06-27 Magnetically activated cell sorting for production of proteins
PCT/US1997/018808 WO1998016541A1 (en) 1996-10-11 1997-10-10 Magnetically activated cell sorting for production of proteins

Publications (2)

Publication Number Publication Date
CA2268397A1 true CA2268397A1 (en) 1998-04-23
CA2268397C CA2268397C (en) 2008-02-12

Family

ID=26703823

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002268397A Expired - Lifetime CA2268397C (en) 1996-10-11 1997-10-10 Magnetically activated cell sorting for production of proteins

Country Status (9)

Country Link
US (1) US5876925A (en)
EP (1) EP0935604B1 (en)
JP (1) JP4101298B2 (en)
AT (1) ATE331727T1 (en)
AU (1) AU722978B2 (en)
CA (1) CA2268397C (en)
DE (1) DE69736239T2 (en)
ES (1) ES2267134T3 (en)
WO (1) WO1998016541A1 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6255455B1 (en) * 1996-10-11 2001-07-03 The Trustees Of The University Of Pennsylvania Rh(D)-binding proteins and magnetically activated cell sorting method for production thereof
DE10129815A1 (en) * 2001-06-24 2003-01-09 Profos Ag Process for the purification of bacterial cells and cell components
EP1487969A4 (en) * 2001-12-07 2008-07-09 Dyax Corp Method and apparatus for washing magnetically responsive particles
JP4601427B2 (en) * 2002-09-18 2010-12-22 ザ・トラステイーズ・オブ・ザ・ユニバーシテイ・オブ・ペンシルベニア Compositions, methods and kits for the detection of antigens on cells and in biological mixtures
DE102004033811A1 (en) * 2004-07-12 2006-02-02 Salama, Abdulgabar, Prof. Dr. Method for the simple and rapid detection of cells and biomolecules with the aid of paramagnetic particles
AU2005263331B8 (en) * 2004-07-20 2011-06-16 Symphogen A/S Anti-Rhesus D recombinant polyclonal antibody and methods of manufacture
BRPI0513714A (en) 2004-07-20 2008-05-13 Symphogen As procedure for structural characterization of a recombinant polyclonal protein or polyclonal cell line
EP1963494B1 (en) 2005-11-29 2014-10-08 The Trustees of The University of Pennsylvania Phage particle diagnostic reagents
US20100006501A1 (en) * 2006-05-05 2010-01-14 Erysave Ab Method for separation
EP2537589A1 (en) 2011-06-21 2012-12-26 Siemens Aktiengesellschaft Method for separating a material from a flowable primary material, device for separating a material from a flowable primary material and control and/or regulating device
RU2678421C2 (en) * 2013-06-28 2019-01-28 Экс-Боди, Инк. Target antigen discovery, phenotypic screens and use thereof for identification of target cell specific target epitopes
US11591718B2 (en) 2018-04-27 2023-02-28 Aimed Bio Inc. Magnetic-based biopanning method through attachment of magnetic bead to cell

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5338689A (en) * 1987-08-24 1994-08-16 Stiftung Fur Diagnostische Forschung Method and card for detecting antigens and/or antibodies
US5663143A (en) * 1988-09-02 1997-09-02 Dyax Corp. Engineered human-derived kunitz domains that inhibit human neutrophil elastase
US5498538A (en) * 1990-02-15 1996-03-12 The University Of North Carolina At Chapel Hill Totally synthetic affinity reagents
DK1471142T3 (en) * 1991-04-10 2009-03-09 Scripps Research Inst Heterodimeric receptor libraries using phagemids
US5665558A (en) * 1994-05-17 1997-09-09 Gamma Biologicals, Inc. Method and apparatus useful for detecting bloodgroup antigens and antibodies

Also Published As

Publication number Publication date
AU722978B2 (en) 2000-08-17
EP0935604A1 (en) 1999-08-18
DE69736239T2 (en) 2007-05-10
JP4101298B2 (en) 2008-06-18
EP0935604A4 (en) 2004-10-20
JP2002503085A (en) 2002-01-29
DE69736239D1 (en) 2006-08-10
AU4824797A (en) 1998-05-11
WO1998016541A1 (en) 1998-04-23
ES2267134T3 (en) 2007-03-01
EP0935604B1 (en) 2006-06-28
ATE331727T1 (en) 2006-07-15
CA2268397C (en) 2008-02-12
US5876925A (en) 1999-03-02

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Legal Events

Date Code Title Description
EEER Examination request
FZDC Discontinued application reinstated
MKEX Expiry

Effective date: 20171010