CA2289283A1 - Metastasis models using green fluorescent protein (gfp) as a marker - Google Patents
Metastasis models using green fluorescent protein (gfp) as a marker Download PDFInfo
- Publication number
- CA2289283A1 CA2289283A1 CA002289283A CA2289283A CA2289283A1 CA 2289283 A1 CA2289283 A1 CA 2289283A1 CA 002289283 A CA002289283 A CA 002289283A CA 2289283 A CA2289283 A CA 2289283A CA 2289283 A1 CA2289283 A1 CA 2289283A1
- Authority
- CA
- Canada
- Prior art keywords
- gfp
- tumor
- contain
- metastasis
- tumors
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 206010027476 Metastases Diseases 0.000 title abstract 3
- 230000009401 metastasis Effects 0.000 title abstract 3
- 108010043121 Green Fluorescent Proteins Proteins 0.000 title 1
- 102000004144 Green Fluorescent Proteins Human genes 0.000 title 1
- 239000005090 green fluorescent protein Substances 0.000 title 1
- 239000003550 marker Substances 0.000 title 1
- 206010028980 Neoplasm Diseases 0.000 abstract 6
- 241000251539 Vertebrata <Metazoa> Species 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 230000033115 angiogenesis Effects 0.000 abstract 1
- 239000000994 contrast dye Substances 0.000 abstract 1
- 238000011065 in-situ storage Methods 0.000 abstract 1
- 210000000056 organ Anatomy 0.000 abstract 1
- 210000004881 tumor cell Anatomy 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/001—Preparation for luminescence or biological staining
- A61K49/0013—Luminescence
- A61K49/0017—Fluorescence in vivo
- A61K49/0019—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules
- A61K49/0045—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules the fluorescent agent being a peptide or protein used for imaging or diagnosis in vivo
- A61K49/0047—Green fluorescent protein [GFP]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/027—New breeds of vertebrates
- A01K67/0271—Chimeric animals, e.g. comprising exogenous cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/001—Preparation for luminescence or biological staining
- A61K49/0013—Luminescence
- A61K49/0017—Fluorescence in vivo
- A61K49/0019—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules
- A61K49/0021—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules the fluorescent group being a small organic molecule
- A61K49/0041—Xanthene dyes, used in vivo, e.g. administered to a mice, e.g. rhodamines, rose Bengal
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
Abstract
A method to follow the progression of metastasis of a primary tumor, which method comprises removing fresh organ tissues from a vertebrate subject which has been modified to contain tumor cells that express GFP and observing the excised tissues for the presence of fluorescence is disclosed. The fluorescence can also be monitored by observing the tissues in situ. Vertebrate subjects which contain GFP
producing tumors are useful models to study the mechanism of metastasis.
In addition, subjects already harboring tumors can be treated so as to modify the endogenous tumors to contain GFP. This permits clinical applications. Finally, by injecting a contrast dye into a subject harboring a GFP-labeled tumor, angiogenesis in the tumor can be observed directly.
producing tumors are useful models to study the mechanism of metastasis.
In addition, subjects already harboring tumors can be treated so as to modify the endogenous tumors to contain GFP. This permits clinical applications. Finally, by injecting a contrast dye into a subject harboring a GFP-labeled tumor, angiogenesis in the tumor can be observed directly.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/848,539 US6232523B1 (en) | 1997-04-28 | 1997-04-28 | Metastasis models using green fluorescent protein (GFP) as a marker |
US08/848,539 | 1997-04-28 | ||
US09/049,544 | 1998-03-27 | ||
US09/049,544 US6235967B1 (en) | 1997-04-28 | 1998-03-27 | Metastasis models using green fluorescent protein as a marker |
PCT/US1998/008457 WO1998049336A1 (en) | 1997-04-28 | 1998-04-28 | Metastasis models using green fluorescent protein (gfp) as a marker |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2289283A1 true CA2289283A1 (en) | 1998-11-05 |
CA2289283C CA2289283C (en) | 2009-08-11 |
Family
ID=26727289
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002289283A Expired - Fee Related CA2289283C (en) | 1997-04-28 | 1998-04-28 | Metastasis models using green fluorescent protein (gfp) as a marker |
Country Status (8)
Country | Link |
---|---|
US (1) | US6235968B1 (en) |
EP (1) | EP0979298B1 (en) |
JP (2) | JP2001517090A (en) |
CN (1) | CN1264429A (en) |
AU (1) | AU749338B2 (en) |
CA (1) | CA2289283C (en) |
DE (1) | DE69839603D1 (en) |
WO (1) | WO1998049336A1 (en) |
Families Citing this family (39)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6235968B1 (en) | 1997-04-28 | 2001-05-22 | Anticancer, Inc. | Metastasis models using green fluorescent protein (GFP) as a marker |
US6251384B1 (en) | 1997-04-28 | 2001-06-26 | Anticancer, Inc. | Metastasis models using green fluorescent protein (GFP) as a marker |
US6232523B1 (en) | 1997-04-28 | 2001-05-15 | Anticancer, Inc. | Metastasis models using green fluorescent protein (GFP) as a marker |
FR2796393B1 (en) * | 1999-07-15 | 2003-10-31 | Inst Nat Sante Rech Med | MODEL FOR STUDYING THE PROGRESSION OF SPONTANEOUS PULMONARY METASTASES, AND ITS USE IN THE SCREENING OF DRUGS |
EP1294906B1 (en) | 2000-03-17 | 2009-01-21 | Anticancer, Inc. | Whole-body optical imaging of gene expression and uses thereof |
AU2002251830A1 (en) * | 2001-01-26 | 2002-08-06 | Colorado State University Research Foundation | Analysis of gene expression and biological function using optical imaging |
US20020132318A1 (en) * | 2001-01-29 | 2002-09-19 | Ming Zhao | Fluorescent proteins |
JP2005520781A (en) * | 2001-07-09 | 2005-07-14 | アンチキャンサー インコーポレーテッド | Imaging infection using fluorescent proteins as markers |
EP1281767A3 (en) * | 2001-07-31 | 2003-05-28 | Aladar A. Szalay | Light emitting microorganisms and cells for diagnosis and therapy of tumors |
AU2002326872B2 (en) * | 2001-09-10 | 2008-07-03 | Anticancer, Inc. | Enhanced resolution of tumor metastasis |
US7280680B2 (en) | 2002-03-04 | 2007-10-09 | Riken | Method and apparatus for observing three-dimensional localizations of in vivo expressed genes as well as method and apparatus for observing minute three-dimensional localizations of in vivo expressed genes |
US20030228261A1 (en) * | 2002-06-05 | 2003-12-11 | Aladar Szalay | Light emitting microorganisms and cells for diagnosis and therapy of diseases associated with wounded or inflamed tissue |
EP1369491A1 (en) * | 2002-06-05 | 2003-12-10 | Aladar A. Szalay | Light emitting microorganisms and cells for diagnosis and therapy of diseases associated with wounded or inflamed tissue |
CA2495411C (en) * | 2002-08-16 | 2013-12-10 | Anticancer, Inc. | Real time measurement of cellular responses |
WO2004106497A2 (en) * | 2003-05-27 | 2004-12-09 | Anticancer, Inc. | Imageable animal model of sars infection |
EP1631935A4 (en) * | 2003-06-09 | 2008-10-15 | Univ Singapore | System and method for providing a service |
AU2004289953B2 (en) | 2003-06-18 | 2008-09-25 | Genelux Corporation | Modified recombinant vaccina viruses and other microorganisms, uses thereof |
CN1295340C (en) * | 2003-08-13 | 2007-01-17 | 上海南方模式生物科技发展有限公司 | Method for extablishing polymorphism adenoid tumour mouse model |
US8634607B2 (en) | 2003-09-23 | 2014-01-21 | Cambridge Research & Instrumentation, Inc. | Spectral imaging of biological samples |
US7321791B2 (en) | 2003-09-23 | 2008-01-22 | Cambridge Research And Instrumentation, Inc. | Spectral imaging of deep tissue |
US7155274B1 (en) | 2003-11-21 | 2006-12-26 | Imaging Diagnostic Systems, Inc. | Optical computed tomography scanner for small laboratory animals |
CN101151623B (en) | 2005-01-27 | 2010-06-23 | 剑桥研究和仪器设备股份有限公司 | Method and equipment for classifying sample image features |
EP1954809B1 (en) * | 2005-09-30 | 2011-08-03 | AntiCancer, Inc. | Methods for indentifying markers for early-stage human cancer, cancer progression and recurrence |
KR100912784B1 (en) | 2006-01-05 | 2009-08-18 | 엘지전자 주식회사 | Data transmission method and data retransmission method |
US8493854B2 (en) | 2006-02-07 | 2013-07-23 | Lg Electronics Inc. | Method for avoiding collision using identifier in mobile network |
EP2037270A3 (en) * | 2006-03-31 | 2009-03-25 | F. Hoffmann-La Roche Ag | Tumor models employing green fluorescent protein |
KR20070121505A (en) | 2006-06-21 | 2007-12-27 | 엘지전자 주식회사 | Method for reconfiguring radio link |
TW200819540A (en) | 2006-07-11 | 2008-05-01 | Genelux Corp | Methods and compositions for detection of microorganisms and cells and treatment of diseases and disorders |
EP2415783B1 (en) | 2006-10-16 | 2016-12-14 | Genelux Corporation | Modified vaccinia virus strains for use in a diagnostic and therapeutic method |
CA2690627C (en) * | 2007-06-15 | 2014-12-02 | Genelux Corporation | Microorganisms for imaging and/or treatment of tumors |
CA2700543A1 (en) * | 2007-10-01 | 2009-04-09 | Anticancer, Inc. | Imageable rodent model of asthma |
WO2012142529A2 (en) | 2011-04-15 | 2012-10-18 | Genelux Corporation | Clonal strains of attenuated vaccinia viruses and methods of use thereof |
RU2562878C1 (en) * | 2014-07-01 | 2015-09-10 | Михаил Юрьевич Бяхов | Method for simulating serrated colon adenoma |
CA3102210C (en) | 2015-11-02 | 2022-06-14 | Pura Scents, Inc. | Scent dispensation |
USD809116S1 (en) | 2015-11-02 | 2018-01-30 | Pura Scents | Dispenser |
USD816506S1 (en) | 2015-11-02 | 2018-05-01 | Pura Scents, Inc. | Vial for a scent dispenser |
CN107361024A (en) * | 2017-07-18 | 2017-11-21 | 郑州大学 | A kind of method for building up of esophageal squamous cell carcinoma early stage metastasis model |
CN108795872B (en) * | 2018-06-05 | 2022-05-27 | 南方医科大学 | In-vitro tumor cell 3D collective invasion model and construction method thereof |
CN110157684A (en) * | 2019-05-30 | 2019-08-23 | 浙江省肿瘤医院 | RFP marks small cell lung cancer cell system and its foundation and application |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5650135A (en) | 1994-07-01 | 1997-07-22 | The Board Of Trustees Of The Leland Stanford Junior University | Non-invasive localization of a light-emitting conjugate in a mammal |
WO1997045550A2 (en) | 1996-05-31 | 1997-12-04 | Baxter International Inc. | Mini-adenoviral vector |
US6235968B1 (en) | 1997-04-28 | 2001-05-22 | Anticancer, Inc. | Metastasis models using green fluorescent protein (GFP) as a marker |
-
1998
- 1998-04-28 US US09/067,734 patent/US6235968B1/en not_active Expired - Fee Related
- 1998-04-28 CA CA002289283A patent/CA2289283C/en not_active Expired - Fee Related
- 1998-04-28 DE DE69839603T patent/DE69839603D1/en not_active Expired - Lifetime
- 1998-04-28 CN CN98804513.3A patent/CN1264429A/en active Pending
- 1998-04-28 EP EP98918790A patent/EP0979298B1/en not_active Expired - Lifetime
- 1998-04-28 WO PCT/US1998/008457 patent/WO1998049336A1/en active IP Right Grant
- 1998-04-28 AU AU71648/98A patent/AU749338B2/en not_active Ceased
- 1998-04-28 JP JP54725098A patent/JP2001517090A/en active Pending
-
2004
- 2004-08-24 JP JP2004244330A patent/JP2005006660A/en active Pending
Also Published As
Publication number | Publication date |
---|---|
AU7164898A (en) | 1998-11-24 |
JP2005006660A (en) | 2005-01-13 |
EP0979298A1 (en) | 2000-02-16 |
JP2001517090A (en) | 2001-10-02 |
WO1998049336A1 (en) | 1998-11-05 |
DE69839603D1 (en) | 2008-07-24 |
CA2289283C (en) | 2009-08-11 |
US6235968B1 (en) | 2001-05-22 |
CN1264429A (en) | 2000-08-23 |
AU749338B2 (en) | 2002-06-27 |
EP0979298B1 (en) | 2008-06-11 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKLA | Lapsed |
Effective date: 20180430 |