CA2313380A1 - Method for creating polynucleotide and polypeptide sequences - Google Patents

Method for creating polynucleotide and polypeptide sequences Download PDF

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Publication number
CA2313380A1
CA2313380A1 CA002313380A CA2313380A CA2313380A1 CA 2313380 A1 CA2313380 A1 CA 2313380A1 CA 002313380 A CA002313380 A CA 002313380A CA 2313380 A CA2313380 A CA 2313380A CA 2313380 A1 CA2313380 A1 CA 2313380A1
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CA
Canada
Prior art keywords
heteroduplexes
polypeptide sequences
polynucleotide variants
polynucleotide
desired property
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002313380A
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French (fr)
Other versions
CA2313380C (en
Inventor
Frances Arnold
Zhixin Shao
Alexander Volkov
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California Institute of Technology CalTech
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California Institute Of Technology
Frances Arnold
Zhixin Shao
Alexander Volkov
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Publication of CA2313380A1 publication Critical patent/CA2313380A1/en
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Publication of CA2313380C publication Critical patent/CA2313380C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries

Abstract

The invention provides methods for evolving a polynucleotide toward acquisition of a desired property. Such methods entail incubating a population of parental polynucleotide variants under conditions to generate annealed polynucleotides comprising heteroduplexes. The heteroduplexes are then exposed to a cellular DNA repair system to convert the heteroduplexes to parental polynucleotide variants or recombined polynucleotide variants. The resulting polynucleotides are then screened or selected for the desired property.
CA002313380A 1997-12-08 1998-12-04 Method for creating polynucleotide and polypeptide sequences Expired - Lifetime CA2313380C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US6790897P 1997-12-08 1997-12-08
US60/067,908 1997-12-08
PCT/US1998/025698 WO1999029902A1 (en) 1997-12-08 1998-12-04 Method for creating polynucleotide and polypeptide sequences

Publications (2)

Publication Number Publication Date
CA2313380A1 true CA2313380A1 (en) 1999-06-17
CA2313380C CA2313380C (en) 2008-12-30

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CA002313380A Expired - Lifetime CA2313380C (en) 1997-12-08 1998-12-04 Method for creating polynucleotide and polypeptide sequences

Country Status (9)

Country Link
US (3) US6537746B2 (en)
EP (2) EP2270234B1 (en)
JP (1) JP3712255B2 (en)
KR (1) KR20010032861A (en)
AU (1) AU746786B2 (en)
CA (1) CA2313380C (en)
DK (2) DK2270234T3 (en)
IL (1) IL136574A0 (en)
WO (1) WO1999029902A1 (en)

Families Citing this family (202)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
AU1124499A (en) 1997-10-28 1999-05-17 Maxygen, Inc. Human papillomavirus vectors
EP1030861A4 (en) 1997-10-31 2001-09-05 Maxygen Inc Modification of virus tropism and host range by viral genome shuffling
US6531308B2 (en) 1997-11-04 2003-03-11 Eli Lilly And Company Ketoreductase gene and protein from yeast
AU746786B2 (en) 1997-12-08 2002-05-02 California Institute Of Technology Method for creating polynucleotide and polypeptide sequences
JP2002517995A (en) 1998-06-17 2002-06-25 マキシジェン， インコーポレイテッド Method for producing a polynucleotide having desired properties
US7332275B2 (en) * 1999-10-13 2008-02-19 Sequenom, Inc. Methods for detecting methylated nucleotides
DE19953854C2 (en) * 1999-11-09 2002-01-17 Max Planck Gesellschaft Process for the production of biopolymers with modified properties
WO2001064864A2 (en) * 2000-02-28 2001-09-07 Maxygen, Inc. Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation
AU2001272978A1 (en) 2000-06-23 2002-01-08 Maxygen, Inc. Novel co-stimulatory molecules
WO2002000897A2 (en) 2000-06-23 2002-01-03 Maxygen, Inc. Novel chimeric promoters
US6858422B2 (en) 2000-07-13 2005-02-22 Codexis, Inc. Lipase genes
AU2001280968A1 (en) 2000-07-31 2002-02-13 Menzel, Rolf Compositions and methods for directed gene assembly
JP2004509628A (en) * 2000-09-21 2004-04-02 メルク　エンド　カムパニー　インコーポレーテッド Method for producing recombinant polynucleotide
US6783941B2 (en) 2000-12-06 2004-08-31 Novozymes A/S Method for producing a polynucleotide library in vitro by mismatch repair of heteroduplexes
DE60234159D1 (en) * 2001-02-02 2009-12-10 Large Scale Biology Corp METHOD OF INCREASING COMPLEMENTARITY IN A HETERODUPLEX POLYNUCLEOTIDE
US20040142433A1 (en) * 2001-02-02 2004-07-22 Padgett Hal S. Polynucleotide sequence variants
US7582423B2 (en) 2001-02-02 2009-09-01 Novici Biotech Llc Population of polynucleotide sequence variants
US7838219B2 (en) * 2001-02-02 2010-11-23 Novici Biotech Llc Method of increasing complementarity in a heteroduplex
US7465567B2 (en) * 2001-04-16 2008-12-16 California Institute Of Technology Peroxide-driven cytochrome P450 oxygenase variants
DK1404830T3 (en) 2001-05-17 2008-11-10 Proteus Process for preparing polynucleotide fragments for use in shuffling
WO2003010311A2 (en) * 2001-07-23 2003-02-06 Dsm Ip Assets B.V. Process for preparing variant polynucleotides
US20030157495A1 (en) * 2002-02-01 2003-08-21 Padgett Hal S. Nucleic acid molecules encoding CEL I endonuclease and methods of use thereof
US7078211B2 (en) * 2002-02-01 2006-07-18 Large Scale Biology Corporation Nucleic acid molecules encoding endonucleases and methods of use thereof
JP2006508632A (en) * 2002-03-01 2006-03-16 ラブジェン， インコーポレイテッド Methods for detecting genetic diseases
US6977162B2 (en) * 2002-03-01 2005-12-20 Ravgen, Inc. Rapid analysis of variations in a genome
DK2315145T3 (en) 2002-03-01 2016-01-25 Codexis Mayflower Holdings Llc Methods, systems, and software for identifying the functional biomolecules
WO2003078583A2 (en) 2002-03-09 2003-09-25 Maxygen, Inc. Optimization of crossover points for directed evolution
BRPI0309391B1 (en) 2002-04-19 2018-10-30 Dsm Food Specialties B V vector comprising nucleic acid encoding phospholipase activity polypeptide, transformed cell, protein preparation, preparation method and related methods
US7226771B2 (en) 2002-04-19 2007-06-05 Diversa Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
US7727720B2 (en) * 2002-05-08 2010-06-01 Ravgen, Inc. Methods for detection of genetic disorders
US7442506B2 (en) * 2002-05-08 2008-10-28 Ravgen, Inc. Methods for detection of genetic disorders
US20070178478A1 (en) * 2002-05-08 2007-08-02 Dhallan Ravinder S Methods for detection of genetic disorders
US9321832B2 (en) 2002-06-28 2016-04-26 Domantis Limited Ligand
EP2275536A1 (en) 2002-08-06 2011-01-19 Verdia, Inc. AP1 amine oxidase variants
CA2507189C (en) * 2002-11-27 2018-06-12 Sequenom, Inc. Fragmentation-based methods and systems for sequence variation detection and discovery
EP2194133B1 (en) 2003-03-06 2015-12-02 BASF Enzymes LLC Amylases, nucleic acids encoding them and methods for making and using them
MX295545B (en) 2003-03-07 2012-02-03 Diversa Corp Hydrolases, nucleic acids encoding them and mehods for making and using them.
MXPA05010681A (en) 2003-04-04 2005-12-15 Diversa Corp Pectate lyases, nucleic acids encoding them and methods for making and using them.
AU2004235331B2 (en) * 2003-04-25 2008-12-18 Sequenom, Inc. Fragmentation-based methods and systems for De Novo sequencing
SG155063A1 (en) 2003-04-29 2009-09-30 Pioneer Hi Bred Int Novel glyphosate-n-acetyltransferase (gat) genes
EP1639091B1 (en) 2003-06-17 2012-12-05 California University Of Technology Regio- and enantioselective alkane hydroxylation with modified cytochrome p450
US8603949B2 (en) * 2003-06-17 2013-12-10 California Institute Of Technology Libraries of optimized cytochrome P450 enzymes and the optimized P450 enzymes
US7960148B2 (en) 2003-07-02 2011-06-14 Verenium Corporation Glucanases, nucleic acids encoding them and methods for making and using them
WO2005017116A2 (en) * 2003-08-11 2005-02-24 California Institute Of Technology Thermostable peroxide-driven cytochrome p450 oxygenase variants and methods of use
WO2005021714A2 (en) 2003-08-11 2005-03-10 Diversa Corporation Laccases, nucleic acids encoding them and methods for making and using them
WO2005024068A2 (en) * 2003-09-05 2005-03-17 Sequenom, Inc. Allele-specific sequence variation analysis
US20070207463A1 (en) * 2003-11-20 2007-09-06 Ziduo Liu Method
ES2280891T3 (en) * 2003-12-04 2007-09-16 F. Hoffmann-La Roche Ag METHOD FOR OBTAINING MUTED AND / OR CHEMICAL CIRCULAR POLYUCLEOTIDES.
CA2561381C (en) * 2004-03-26 2015-05-12 Sequenom, Inc. Base specific cleavage of methylation-specific amplification products in combination with mass analysis
CN101001957B (en) 2004-06-09 2012-07-04 先锋高级育种国际公司 Plastid transit peptides
ES2461857T3 (en) 2004-06-16 2014-05-21 Dsm Ip Assets B.V. Compositions and methods for enzymatic discoloration of chlorophyll
WO2006031745A2 (en) * 2004-09-10 2006-03-23 Sequenom, Inc. Methods for long-range sequence analysis of nucleic acids
US20090038023A1 (en) 2005-03-10 2009-02-05 Verenium Corporation Lyase Enzymes, Nucleic Acids Encoding Them and Methods For Making and Using Them
AU2006227965B2 (en) 2005-03-15 2013-01-31 Bp Corporation North America Inc. Cellulases, nucleic acids encoding them and methods for making and using them
US8715988B2 (en) * 2005-03-28 2014-05-06 California Institute Of Technology Alkane oxidation by modified hydroxylases
WO2007037676A1 (en) * 2005-09-29 2007-04-05 Keygene N.V. Method and means for targeted nucleotide exchange
US20070161031A1 (en) * 2005-12-16 2007-07-12 The Board Of Trustees Of The Leland Stanford Junior University Functional arrays for high throughput characterization of gene expression regulatory elements
WO2007087301A2 (en) * 2006-01-23 2007-08-02 Zygo Corporation Interferometer system for monitoring an object
EP2216403A3 (en) 2006-02-02 2010-11-24 Verenium Corporation Esterases and related nucleic acids and methods
DK1989301T3 (en) 2006-02-10 2015-01-05 Bp Corp North America Inc Cellulolytic enzymes, nucleic acids encoding them, and methods for making and using them
CA2638801C (en) 2006-02-14 2016-12-13 Verenium Corporation Xylanases, nucleic acids encoding them and methods for making and using them
EA019971B1 (en) 2006-03-07 2014-07-30 Верениум Корпорейшн Aldolases, nucleic acids encoding them and methods for making and using them
MX2008011477A (en) 2006-03-07 2008-09-23 Cargill Inc Aldolases, nucleic acids encoding them and methods for making and using them.
US8252559B2 (en) * 2006-08-04 2012-08-28 The California Institute Of Technology Methods and systems for selective fluorination of organic molecules
US8026085B2 (en) * 2006-08-04 2011-09-27 California Institute Of Technology Methods and systems for selective fluorination of organic molecules
CN103397043A (en) 2006-08-04 2013-11-20 维莱尼姆公司 Glucanases, nucleic acids encoding same and method for making and using same
CA2663001A1 (en) 2006-09-21 2008-03-27 Verenium Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
DK2617814T3 (en) 2006-09-21 2016-02-22 Basf Enzymes Llc Phytases, nucleic acids encoding them, and methods of producing and using them
EP2479267B1 (en) 2006-12-21 2016-12-21 BASF Enzymes LLC Amylases and glucoamylases, nucleic acids encoding them and methods for making and using them
JP2010515683A (en) * 2007-01-05 2010-05-13 ザ カリフォルニア インスティチュート オブ テクノロジー Method for producing new stabilized proteins
AR065544A1 (en) 2007-01-30 2009-06-17 Verenium Corp ENZYMES FOR THE TREATMENT OF NUCLEIC ACID LIGNOCELLULOSICS THAT CODE AND METHODS TO PREPARE AND USE THEM
WO2008121435A2 (en) * 2007-02-02 2008-10-09 The California Institute Of Technology Methods for generating novel stabilized proteins
WO2008103248A1 (en) 2007-02-08 2008-08-28 Codexis, Inc. Ketoreductases and uses thereof
US20080268517A1 (en) * 2007-02-08 2008-10-30 The California Institute Of Technology Stable, functional chimeric cytochrome p450 holoenzymes
US20080220983A1 (en) * 2007-03-08 2008-09-11 Switchgear Genomics A California Corporation Functional arrays for high throughput characterization of regulatory elements in untranslated regions of genes
CN101600797A (en) * 2007-04-25 2009-12-09 乔苗 Site-directed mutagenesis in the circular methylated DNA
US20100267147A1 (en) * 2007-04-25 2010-10-21 GM Biosciences, Inc. Site-directed mutagenesis in circular methylated dna
US8802401B2 (en) * 2007-06-18 2014-08-12 The California Institute Of Technology Methods and compositions for preparation of selectively protected carbohydrates
CN101784669B (en) 2007-08-24 2015-02-18 科德克希思公司 Improved ketoreductase polypeptides for the stereoselective production of (R)-3-hydroxythiolane
MX2010003600A (en) 2007-10-03 2010-12-14 Verenium Corp Xylanases, nucleic acids encoding them and methods for making and using them.
EP2238261B1 (en) 2008-01-03 2013-12-04 Verenium Corporation Isomerases, nucleic acids encoding them and methods for making and using them
US8709772B2 (en) 2008-01-03 2014-04-29 Verenium Corporation Transferases and oxidoreductases, nucleic acids encoding them and methods for making and using them
EP2250594B1 (en) 2008-02-12 2017-04-19 Codexis, Inc. Method of generating an optimized, diverse population of variants
EP2250595B1 (en) 2008-02-12 2017-06-14 Codexis, Inc. Method of selecting an optimized diverse population of variants
CA2990650C (en) 2008-05-23 2021-02-09 Pioneer Hi-Bred International, Inc. Novel dgat genes for increased seed storage lipid production and altered fatty acid profiles in oilseed plants
US20090312196A1 (en) 2008-06-13 2009-12-17 Codexis, Inc. Method of synthesizing polynucleotide variants
ES2575005T3 (en) 2008-06-13 2016-06-23 Codexis, Inc. Synthesis method of polynucleotide variants
CN102131813B (en) 2008-06-24 2014-07-30 科德克希思公司 Biocatalytic processes for preparation of substantially stereomerically pure fused bicyclic proline compounds
SI2329013T1 (en) 2008-08-27 2016-03-31 Codexis, Inc. Ketoreductase polypeptides for the production of a 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine
US8198062B2 (en) 2008-08-29 2012-06-12 Dsm Ip Assets B.V. Hydrolases, nucleic acids encoding them and methods for making and using them
WO2010045002A2 (en) 2008-09-26 2010-04-22 Tocagen Inc. Gene therapy vectors and cytosine deaminases
WO2010081053A2 (en) 2009-01-08 2010-07-15 Codexis, Inc. Transaminase polypeptides
BRPI1010239A2 (en) 2009-03-31 2016-10-11 Codexis Inc improved endoglucanases, derivatives and their uses
NZ596459A (en) 2009-05-21 2013-11-29 Verenium Corp Phytases, nucleic acids encoding them and methods for making and using them
CN102482648B (en) 2009-06-22 2014-12-10 科德克希思公司 Ketoreductase-mediated stereoselective route to alpha chloroalcohols
EP2467473B1 (en) 2009-08-19 2016-03-23 Codexis, Inc. Ketoreductase polypeptides for the preparation of phenylephrine
US8815779B2 (en) 2009-09-16 2014-08-26 SwitchGear Genomics, Inc. Transcription biomarkers of biological responses and methods
EP3301208B1 (en) 2009-09-18 2020-11-04 Codexis, Inc. Reduced codon mutagenesis
US20110082055A1 (en) * 2009-09-18 2011-04-07 Codexis, Inc. Reduced codon mutagenesis
UA111708C2 (en) 2009-10-16 2016-06-10 Бандж Ойлз, Інк. METHOD OF OIL REFINING
UA109884C2 (en) 2009-10-16 2015-10-26 A POLYPEPTIDE THAT HAS THE ACTIVITY OF THE PHOSPHATIDYLINOSYTOL-SPECIFIC PHOSPHOLIPASE C, NUCLEIC ACID, AND METHOD OF METHOD
EP2510089B1 (en) 2009-12-08 2015-10-21 Codexis, Inc. Synthesis of prazole compounds
EP2566497B1 (en) 2010-05-04 2015-07-29 Codexis, Inc. Biocatalysts for ezetimibe synthesis
DK2582799T3 (en) 2010-06-17 2018-02-12 Codexis Inc BIOCATALATORS AND PROCEDURES FOR THE SYNTHESIS OF (S) -3- (1-AMINOETHYL) -PHENOL
EP2590991B1 (en) 2010-06-30 2016-01-20 Codexis, Inc. Highly stable beta-class carbonic anhydrases useful in carbon capture systems
US9187762B2 (en) 2010-08-13 2015-11-17 Pioneer Hi-Bred International, Inc. Compositions and methods comprising sequences having hydroxyphenylpyruvate dioxygenase (HPPD) activity
CA2808098C (en) 2010-08-16 2020-03-10 Merck Sharp & Dohme Corp. Process for preparing aminocyclohexyl ether compounds
CN102443561B (en) * 2010-10-13 2014-08-27 上海医药工业研究院 Gene engineering bacterium for efficiently converting Echinocandin B and preparation method thereof
WO2012078800A2 (en) 2010-12-08 2012-06-14 Codexis, Inc. Biocatalysts and methods for the synthesis of armodafinil
EP2697662B1 (en) 2011-04-13 2018-06-06 Codexis, Inc. Biocatalytic process for preparing eslicarbazepine and analogs thereof
US9322007B2 (en) 2011-07-22 2016-04-26 The California Institute Of Technology Stable fungal Cel6 enzyme variants
CN103998461B (en) 2011-09-08 2018-02-02 科德克希思公司 For the biocatalyst and method of the lactams for synthesizing substitution
US9139819B2 (en) 2011-11-18 2015-09-22 Codexis, Inc. Biocatalysts for the preparation of hydroxy substituted carbamates
CN104508126B (en) 2012-03-23 2017-06-30 科德克希思公司 Biocatalyst and method for synthesizing the derivative of tryptamines and tryptamine analogues
CA2872096A1 (en) 2012-05-04 2013-11-07 E. I. Du Pont De Nemours And Company Compositions and methods comprising sequences having meganuclease activity
US9790527B2 (en) 2012-05-08 2017-10-17 Codexis, Inc. Engineered proline hydroxylase polypeptides
WO2013170050A1 (en) 2012-05-11 2013-11-14 Codexis, Inc. Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
EP2912169A4 (en) 2012-10-29 2016-11-23 Univ Rochester Artemisinin derivatives, methods for their preparation and their use as antimalarial agents
DK2928865T3 (en) 2012-12-07 2018-06-18 Merck Sharp & Dohme BIOCATALYTIC TRANSAMINATION PROCEDURE
CA2895752C (en) 2012-12-21 2021-09-21 Codexis, Inc. Engineered biocatalysts and methods for synthesizing chiral amines
EP2946006B1 (en) 2013-01-18 2019-04-10 Codexis, Inc. Engineered biocatalysts useful for carbapenem synthesis
US9684771B2 (en) 2013-01-31 2017-06-20 Codexis, Inc. Methods, systems, and software for identifying bio-molecules using models of multiplicative form
CA2902824C (en) 2013-02-28 2021-06-22 Codexis, Inc. Engineered transaminase polypeptides for industrial biocatalysis
US20140289906A1 (en) 2013-03-14 2014-09-25 Pioneer Hi-Bred International, Inc. Compositions Having Dicamba Decarboxylase Activity and Methods of Use
AU2014236162A1 (en) 2013-03-14 2015-09-17 Arzeda Corp. Compositions having dicamba decarboxylase activity and methods of use
RU2015143825A (en) 2013-03-15 2017-04-26 Пайонир Хай-Бред Интернэшнл, Инк. PHI-4 POLYPEPTIDES AND WAYS OF THEIR APPLICATION
MY193198A (en) 2013-04-18 2022-09-26 Codexis Inc Engineered phenylalanine ammonia lyase polypeptides
EA030896B1 (en) 2013-08-16 2018-10-31 Пайонир Хай-Бред Интернэшнл, Инк. Insecticidal proteins and methods for their use
BR122021005579B1 (en) 2013-09-13 2022-11-29 Pioneer Hi-Bred International, Inc DNA CONSTRUCTION, METHOD FOR OBTAINING A TRANSGENIC PLANT, FUSION PROTEIN, METHOD FOR CONTROLLING AN INSECT PEST POPULATION, METHOD FOR INHIBITING THE GROWTH OR KILLING AN INSECT PEST
CN106232619B (en) 2013-11-13 2022-09-09 科德克希思公司 Engineered imine reductases and methods for reductive amination of ketone and amine compounds
WO2015100277A2 (en) 2013-12-23 2015-07-02 University Of Rochester Methods and compositions for ribosomal synthesis of macrocyclic peptides
CN106232620B (en) 2014-02-07 2022-05-13 先锋国际良种公司 Insecticidal proteins and methods of use thereof
EP3705489A1 (en) 2014-02-07 2020-09-09 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
HUE056444T2 (en) 2014-04-16 2022-02-28 Codexis Inc Engineered tyrosine ammonia lyase
EP3167052B1 (en) 2014-07-09 2020-01-01 Codexis, Inc. P450-bm3 variants with improved activity
EP3207143B1 (en) 2014-10-16 2023-11-22 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
WO2016085916A1 (en) 2014-11-25 2016-06-02 Codexis, Inc. Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
KR102438885B1 (en) 2014-12-22 2022-09-01 코덱시스, 인코포레이티드 Human alpha-galactosidase variants
MX2017011525A (en) 2015-03-11 2018-01-30 Pioneer Hi Bred Int Insecticidal combinations of pip-72 and methods of use.
JP6750830B2 (en) * 2015-04-07 2020-09-02 国立大学法人東京工業大学 Method for producing transformed cell
CA2985198A1 (en) 2015-05-19 2016-11-24 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
DK3319987T3 (en) 2015-07-07 2021-06-21 Codexis Inc NEW P450-BM3 VARIANTS WITH IMPROVED ACTIVITY
MX2018001523A (en) 2015-08-06 2018-03-15 Pioneer Hi Bred Int Plant derived insecticidal proteins and methods for their use.
US20180325119A1 (en) 2015-12-18 2018-11-15 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
WO2017192560A1 (en) 2016-05-04 2017-11-09 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
DK3452587T3 (en) 2016-05-05 2021-10-11 Codexis Inc PENICILLIN-G-ACYLASER
CN109715817B (en) 2016-06-09 2022-12-09 科德克希思公司 Biocatalysts and methods for hydroxylation of chemical compounds
WO2017218325A1 (en) 2016-06-15 2017-12-21 Codexis, Inc. Engineered beta-glucosidases and glucosylation methods
EP3478052B1 (en) 2016-07-01 2021-08-25 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
CA3032519A1 (en) 2016-08-26 2018-03-01 Codexis, Inc. Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
BR112019008800A2 (en) 2016-11-01 2019-07-16 Pioneer Hi Bred Int insecticidal polypeptide, insecticidal composition, recombinant polynucleotide, dna construct, plant cell or transgenic plant, method for inhibiting growth or exterminating an agricultural insect pest population, method for inhibiting growth or exterminating an insect pest, method for controlling lepidoptera and / or coleoptera insect infestation in a transgenic plant and provide insect resistance management and use of at least one insecticide polypeptide
WO2018111551A1 (en) 2016-12-14 2018-06-21 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
WO2018118811A1 (en) 2016-12-22 2018-06-28 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP3577229A4 (en) 2017-02-03 2020-12-23 Codexis, Inc. Engineered glycosyltransferases and steviol glycoside glucosylation methods
MX2019009371A (en) 2017-02-08 2019-09-23 Pionner Hi Bred Int Inc Insecticidal combinations of plant derived insecticidal proteins and methods for their use.
BR112019016634A2 (en) 2017-02-13 2020-04-07 Codexis Inc polypeptides, engineered polynucleotide sequence, expression vector, host cell, methods for producing an engineered phenylalanine ammonia lyase polypeptide in a host cell and for treating and / or preventing phenylketonuria symptoms in an individual, composition, and , use
US11161834B2 (en) 2017-04-24 2021-11-02 Tesaro, Inc. Methods of manufacturing of niraparib
CN117511890A (en) 2017-04-27 2024-02-06 科德克希思公司 Ketoreductase polypeptides and polynucleotides
SG11201909957TA (en) 2017-05-08 2019-11-28 Codexis Inc Engineered ligase variants
WO2018208882A1 (en) 2017-05-11 2018-11-15 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
CN111032682B (en) 2017-06-14 2024-04-12 科德克希思公司 Engineered transaminase polypeptides for industrial biocatalysis
CN117737045A (en) 2017-06-27 2024-03-22 科德克希思公司 Penicillin G acylase
US10793841B2 (en) 2017-06-30 2020-10-06 Codexis, Inc. T7 RNA polymerase variants
KR20200023454A (en) 2017-06-30 2020-03-04 코덱시스, 인코포레이티드 T7 RNA Polymerase Variants
US11634400B2 (en) 2017-08-19 2023-04-25 University Of Rochester Micheliolide derivatives, methods for their preparation and their use as anticancer and antiinflammatory agents
CN111511389A (en) 2017-11-07 2020-08-07 科德克希思公司 Transglutaminase variants
US11015180B2 (en) 2017-12-13 2021-05-25 Codexis, Inc. Carboxyesterase polypeptides for amide coupling
WO2019116311A1 (en) 2017-12-13 2019-06-20 Glaxosmithkline Intellectual Property Development Limited Carboxyesterase biocatalysts
WO2019241132A1 (en) 2018-06-12 2019-12-19 Codexis, Inc. Engineered tyrosine ammonia lyase
CN112601821A (en) 2018-07-09 2021-04-02 科德克希思公司 Engineered pentose phosphate mutase variant enzymes
US11274286B2 (en) 2018-07-09 2022-03-15 Codexis, Inc. Engineered deoxyribose-phosphate aldolases
US11162105B2 (en) 2018-07-09 2021-11-02 Codexis, Inc. Engineered purine nucleoside phosphorylase variant enzymes
SG11202012138XA (en) 2018-07-09 2021-01-28 Codexis Inc Engineered galactose oxidase variant enzymes
AU2019300838A1 (en) 2018-07-09 2021-01-07 Codexis, Inc. Engineered pantothenate kinase variant enzymes
US11198861B2 (en) 2018-07-12 2021-12-14 Codexis, Inc. Engineered phenylalanine ammonia lyase polypeptides
US11760981B2 (en) 2018-07-30 2023-09-19 Codexis, Inc. Engineered glycosyltransferases and steviol glycoside glucosylation methods
RU2705256C1 (en) * 2018-09-05 2019-11-06 Общество С Ограниченной Ответственностью "Прорывные Инновационные Технологии" GENE-THERAPEUTIC DNA VECTOR BASED ON THE GENE-THERAPEUTIC DNA VECTOR VTvaf17, CARRYING THE TARGET GENE SELECTED FROM THE GROUP OF SKI, TGFB3, TIMP2, FMOD GENES TO INCREASE THE LEVEL OF EXPRESSION OF THESE TARGET GENES, A METHOD FOR PREPARING AND USING IT, ESCHERICHIA COLI SCS110-AF/VTvaf17-SKI STRAIN OR ESCHERICHIA COLI SCS110-AF/VTvaf17-TGFB3 OR ESCHERICHIA COLI SCS110-AF/VTvaf17-TIMP2 OR ESCHERICHIA COLI SCS110-AF/VTvaf17-FMOD, CARRYING A GENE-THERAPEUTIC DNA VECTOR, A METHOD FOR PRODUCTION THEREOF, A METHOD FOR INDUSTRIAL PRODUCTION OF A GENE-THERAPEUTIC DNA VECTOR
BR112021008186A2 (en) 2018-10-29 2021-08-17 Codexis, Inc. engineered dna polymerase, polynucleotide sequence, expression vector, host cell, composition, high-throughput assay system, and, methods for producing a engineered dna polymerase polypeptide in a host cell and for determining high throughput fidelity of a dna polymerase.
SG11202105668PA (en) 2018-12-14 2021-06-29 Codexis Inc Engineered tyrosine ammonia lyase
EP3898960A4 (en) 2018-12-20 2022-11-30 Codexis, Inc. Human alpha-galactosidase variants
CN109897843B (en) * 2019-03-01 2021-02-02 浙江工业大学 Recombinant echinocandin B deacylase mutant and application thereof
CN110616181A (en) * 2019-09-27 2019-12-27 北京理工大学 Engineering strain based on escherichia coli transformation
WO2021067608A1 (en) 2019-10-02 2021-04-08 Abbott Laboratories Detection of analytes by protein switches
US11788071B2 (en) 2020-04-10 2023-10-17 Codexis, Inc. Engineered transaminase polypeptides
CN115867564A (en) 2020-07-14 2023-03-28 先锋国际良种公司 Insecticidal proteins and methods of use thereof
US11959108B2 (en) 2020-08-28 2024-04-16 Codexis, Inc. Engineered protease variants
BR112023003643A2 (en) 2020-08-28 2023-03-28 Codexis Inc RECOMBINANT AMYLASE, COMPOSITION, RECOMBINANT POLYNUCLEOTIDE SEQUENCE, EXPRESSION VECTOR, HOST CELL, METHODS FOR PRODUCING A RECOMBINANT AMYLASE AND FOR TREAT AND/OR PREVENT THE SYMPTOMS OF PANCREATIC FAILURE, PHARMACEUTICAL COMPOSITION FOR THE TREATMENT OF PANCREATIC FAILURE, AND, USE
US20220186231A1 (en) 2020-12-11 2022-06-16 Willow Biosciences, Inc. Recombinant acyl activating enzyme (aae) genes for enhanced biosynthesis of cannabinoids and cannabinoid precursors
WO2022133289A2 (en) 2020-12-18 2022-06-23 Codexis, Inc. Engineered uridine phosphorylase variant enzymes
CA3214972A1 (en) 2021-04-02 2022-10-06 Codexis, Inc. Engineered adenylate kinase variant enzymes
CA3214975A1 (en) 2021-04-02 2022-10-06 Codexis, Inc. Engineered cyclic gmp-amp synthase (cgas) variant enzymes
CN117222735A (en) 2021-04-02 2023-12-12 科德克希思公司 Engineered acetate kinase variant enzymes
US20220325284A1 (en) 2021-04-02 2022-10-13 Codexis, Inc. Engineered guanylate kinase variant enzymes
WO2023010083A2 (en) 2021-07-30 2023-02-02 Willow Biosciences, Inc. Recombinant prenyltransferase polypeptides engineered for enhanced biosynthesis of cannabinoids
WO2023023621A1 (en) 2021-08-19 2023-02-23 Willow Biosciences, Inc. Recombinant olivetolic acid cyclase polypeptides engineered for enhanced biosynthesis of cannabinoids
US20230183382A1 (en) 2021-10-15 2023-06-15 Cytomx Therapeutics, Inc. Activatable polypeptide complex
TW202323282A (en) 2021-10-15 2023-06-16 美商賽特艾克斯生物製藥公司 Activatable polypeptide complex
WO2023064955A1 (en) 2021-10-15 2023-04-20 Cytomx Therapeutics, Inc. Activatable anti-cd3, anti-egfr, heteromultimeric bispecific polypeptide complex
WO2023069921A1 (en) 2021-10-19 2023-04-27 Epimeron Usa, Inc. Recombinant thca synthase polypeptides engineered for enhanced biosynthesis of cannabinoids
WO2023173084A1 (en) 2022-03-11 2023-09-14 University Of Rochester Cyclopeptibodies and uses thereof

Family Cites Families (107)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4994379A (en) * 1983-03-09 1991-02-19 Cetus Corporation Modified signal peptides
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
US5422266A (en) 1984-12-31 1995-06-06 University Of Georgia Research Foundation, Inc. Recombinant DNA vectors capable of expressing apoaequorin
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5176995A (en) 1985-03-28 1993-01-05 Hoffmann-La Roche Inc. Detection of viruses by amplification and hybridization
AU4434585A (en) 1985-03-30 1986-10-23 Marc Ballivet Method for obtaining dna, rna, peptides, polypeptides or proteins by means of a dna recombinant technique
US4959312A (en) 1985-05-31 1990-09-25 The University Of Tennessee Research Corporation Full spectrum mutagenesis
DE3688920D1 (en) 1985-07-03 1993-09-30 Genencor Int Hybrid polypeptides and process for their preparation.
US5866363A (en) 1985-08-28 1999-02-02 Pieczenik; George Method and means for sorting and identifying biological information
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
DK311186D0 (en) 1986-06-30 1986-06-30 Novo Industri As ENZYMES
US5824469A (en) 1986-07-17 1998-10-20 University Of Washington Method for producing novel DNA sequences with biological activity
US5763192A (en) 1986-11-20 1998-06-09 Ixsys, Incorporated Process for obtaining DNA, RNA, peptides, polypeptides, or protein, by recombinant DNA technique
US5807723A (en) 1987-03-02 1998-09-15 Whitehead Institute For Biomedical Research Homologously recombinant slow growing mycobacteria and uses therefor
US4994368A (en) 1987-07-23 1991-02-19 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
US5066584A (en) * 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
FR2641793B1 (en) 1988-12-26 1993-10-01 Setratech METHOD OF IN VIVO RECOMBINATION OF DNA SEQUENCES HAVING BASIC MATCHING
DD282028A5 (en) 1989-02-16 1990-08-29 Akad Wissenschaften Ddr PROCESS FOR PREPARING THERMOSTABILES, HYBRIDEN BACILLUS BETA-1,3-1,4-GLUCANASE
US5106727A (en) 1989-04-27 1992-04-21 Life Technologies, Inc. Amplification of nucleic acid sequences using oligonucleotides of random sequences as primers
US5043272A (en) 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
US5556750A (en) * 1989-05-12 1996-09-17 Duke University Methods and kits for fractionating a population of DNA molecules based on the presence or absence of a base-pair mismatch utilizing mismatch repair systems
CA2016841C (en) 1989-05-16 1999-09-21 William D. Huse A method for producing polymers having a preselected activity
CA2016842A1 (en) 1989-05-16 1990-11-16 Richard A. Lerner Method for tapping the immunological repertoire
IT1231157B (en) 1989-07-20 1991-11-19 Crc Ricerca Chim NEW FUNCTIONAL HYBRID GENES OF BACILLUS THURINGIENSIS OBTAINED THROUGH IN VIVO RECOMBINATION.
US5574205A (en) 1989-07-25 1996-11-12 Cell Genesys Homologous recombination for universal donor cells and chimeric mammalian hosts
US5023171A (en) 1989-08-10 1991-06-11 Mayo Foundation For Medical Education And Research Method for gene splicing by overlap extension using the polymerase chain reaction
WO1991006570A1 (en) 1989-10-25 1991-05-16 The University Of Melbourne HYBRID Fc RECEPTOR MOLECULES
US5877402A (en) 1990-05-01 1999-03-02 Rutgers, The State University Of New Jersey DNA constructs and methods for stably transforming plastids of multicellular plants and expressing recombinant proteins therein
US5851813A (en) 1990-07-12 1998-12-22 President And Fellows Of Harvard College Primate lentivirus antigenic compositions
WO1992001814A2 (en) 1990-07-24 1992-02-06 F. Hoffmann-La-Roche Ag The reduction of non-specific amplification during in vitro nucleic acid amplification using modified nucleic acid bases
US5169764A (en) 1990-08-08 1992-12-08 Regeneron Pharmaceuticals, Inc. Multitrophic and multifunctional chimeric neurotrophic factors, and nucleic acids and plasmids encoding the chimeras
US5264563A (en) 1990-08-24 1993-11-23 Ixsys Inc. Process for synthesizing oligonucleotides with random codons
JP3306063B2 (en) 1990-08-24 2002-07-24 イグジス， インコーポレイテッド Method for synthesizing oligonucleotides having random codons
JPH06506105A (en) 1990-08-29 1994-07-14 ファーミング　ビーブイ Homologous recombination in mammalian cells
US5770434A (en) 1990-09-28 1998-06-23 Ixsys Incorporated Soluble peptides having constrained, secondary conformation in solution and method of making same
US5871974A (en) 1990-09-28 1999-02-16 Ixsys Inc. Surface expression libraries of heteromeric receptors
US5698426A (en) 1990-09-28 1997-12-16 Ixsys, Incorporated Surface expression libraries of heteromeric receptors
US5858725A (en) 1990-10-10 1999-01-12 Glaxo Wellcome Inc. Preparation of chimaeric antibodies using the recombinant PCR strategy
US5187083A (en) 1990-11-13 1993-02-16 Specialty Laboratories, Inc. Rapid purification of DNA
US5234824A (en) 1990-11-13 1993-08-10 Specialty Laboratories, Inc. Rapid purification of DNA
US5489523A (en) 1990-12-03 1996-02-06 Stratagene Exonuclease-deficient thermostable Pyrococcus furiosus DNA polymerase I
IL100460A (en) 1990-12-20 1997-06-10 Ixsys Method for optimization of binding proteins and nucleic acids encoding a binding protein produced thereby
US5324830A (en) * 1991-03-26 1994-06-28 United States Of America Chimeric protein that has a human RHo Motif and deoxyribonuclease activity
US5795747A (en) 1991-04-16 1998-08-18 Evotec Biosystems Gmbh Process for manufacturing new biopolymers
US5514568A (en) 1991-04-26 1996-05-07 Eli Lilly And Company Enzymatic inverse polymerase chain reaction
US5512463A (en) 1991-04-26 1996-04-30 Eli Lilly And Company Enzymatic inverse polymerase chain reaction library mutagenesis
DK0519338T3 (en) 1991-06-20 1996-10-28 Hoffmann La Roche Improved Methods for Nucleic Acid Amplification
WO1993000103A1 (en) 1991-06-21 1993-01-07 The Wistar Institute Of Anatomy And Biology Chimeric envelope proteins for viral targeting
US6071889A (en) 1991-07-08 2000-06-06 Neurospheres Holdings Ltd. In vivo genetic modification of growth factor-responsive neural precursor cells
US5223408A (en) 1991-07-11 1993-06-29 Genentech, Inc. Method for making variant secreted proteins with altered properties
US5279952A (en) 1991-08-09 1994-01-18 Board Of Regents, The University Of Texas System PCR-based strategy of constructing chimeric DNA molecules
GB9125979D0 (en) 1991-12-06 1992-02-05 Wellcome Found Antibody
US5773267A (en) 1992-02-07 1998-06-30 Albert Einstein College Of Medicine Of Yeshiva University, A Division Of Yeshiva University D29 shuttle phasmids and uses thereof
EP1382386A3 (en) * 1992-02-19 2004-12-01 The Public Health Research Institute Of The City Of New York, Inc. Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
US5843643A (en) 1992-02-21 1998-12-01 Ratner; Paul L. Site-specific transfection of eukaryotic cells using polypeptide-linked recombinant nucleic acid
ATE280219T1 (en) 1992-03-16 2004-11-15 Jacob Nathaniel Wohlstadter MUTAGENESIS AND SELECTION METHODS IN THE SAME HOST CELLS
US5316935A (en) 1992-04-06 1994-05-31 California Institute Of Technology Subtilisin variants suitable for hydrolysis and synthesis in organic media
US5356801A (en) 1992-07-10 1994-10-18 Energy Biosystems Corporation Recombinant DNA encoding a desulfurization biocatalyst
US5360728A (en) 1992-12-01 1994-11-01 Woods Hole Oceanographic Institution (W.H.O.I.) Modified apoaequorin having increased bioluminescent activity
US5571708A (en) 1993-04-19 1996-11-05 Bristol-Myers Squibb Company Thrombin-activatable plasminogen activator
IT1264712B1 (en) 1993-07-13 1996-10-04 Eniricerche Spa BIOLOGICAL SYNTHESIS METHOD OF PEPTIDES
US5556772A (en) 1993-12-08 1996-09-17 Stratagene Polymerase compositions and uses thereof
US5834252A (en) 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US5837458A (en) * 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5928905A (en) 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
US6165793A (en) 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5521077A (en) 1994-04-28 1996-05-28 The Leland Stanford Junior University Method of generating multiple protein variants and populations of protein variants prepared thereby
US5549551A (en) 1994-12-22 1996-08-27 Advanced Cardiovascular Systems, Inc. Adjustable length balloon catheter
US5629179A (en) 1995-03-17 1997-05-13 Novagen, Inc. Method and kit for making CDNA library
JPH11504218A (en) 1995-04-24 1999-04-20 クロマゾーム コーポレーション Methods for generating and screening new metabolic pathways
US5776744A (en) 1995-06-07 1998-07-07 Yale University Methods and compositions for effecting homologous recombination
US5958672A (en) 1995-07-18 1999-09-28 Diversa Corporation Protein activity screening of clones having DNA from uncultivated microorganisms
US6004788A (en) 1995-07-18 1999-12-21 Diversa Corporation Enzyme kits and libraries
US6168919B1 (en) 1996-07-17 2001-01-02 Diversa Corporation Screening methods for enzymes and enzyme kits
US6030779A (en) 1995-07-18 2000-02-29 Diversa Corporation Screening for novel bioactivities
US6057103A (en) 1995-07-18 2000-05-02 Diversa Corporation Screening for novel bioactivities
EP0843725B1 (en) 1995-08-11 2002-04-17 Novozymes A/S Method for preparing polypeptide variants
US5962258A (en) 1995-08-23 1999-10-05 Diversa Corporation Carboxymethyl cellulase fromthermotoga maritima
DE69636225T2 (en) * 1995-08-30 2006-10-12 Basf Plant Science Gmbh STIMULATION OF HOMOLOGOUS RECOMBINATION IN VEGETABLE ORGANISMS BY RECOMBINATION OF PROMOTING ENZYMES
US6051409A (en) 1995-09-25 2000-04-18 Novartis Finance Corporation Method for achieving integration of exogenous DNA delivered by non-biological means to plant cells
US5756316A (en) 1995-11-02 1998-05-26 Genencor International, Inc. Molecular cloning by multimerization of plasmids
US5830696A (en) 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
US5939250A (en) 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
US20030215798A1 (en) 1997-06-16 2003-11-20 Diversa Corporation High throughput fluorescence-based screening for novel enzymes
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US6352842B1 (en) 1995-12-07 2002-03-05 Diversa Corporation Exonucease-mediated gene assembly in directed evolution
US6171820B1 (en) 1995-12-07 2001-01-09 Diversa Corporation Saturation mutagenesis in directed evolution
US6358709B1 (en) 1995-12-07 2002-03-19 Diversa Corporation End selection in directed evolution
US6361974B1 (en) 1995-12-07 2002-03-26 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
US6096548A (en) 1996-03-25 2000-08-01 Maxygen, Inc. Method for directing evolution of a virus
US5783431A (en) 1996-04-24 1998-07-21 Chromaxome Corporation Methods for generating and screening novel metabolic pathways
US5763239A (en) 1996-06-18 1998-06-09 Diversa Corporation Production and use of normalized DNA libraries
JPH1066577A (en) 1996-08-07 1998-03-10 Novo Nordisk As Nucleic acid pool and its production
US6093873A (en) 1996-08-19 2000-07-25 Institut National De La Sante Et De La Recherche Medicale Genetically engineered mice containing alterations in the gene encoding RXR
IL130635A0 (en) * 1997-01-17 2000-06-01 Maxygen Inc Evolution of whole cells and organisms by recursive sequence recombination
PT981637E (en) 1997-03-14 2005-09-30 Biogen Idec Inc METHOD FOR INTEGRATING GENES IN SPECIFIC SITES IN MAMIFERO CELLS THROUGH RECOMBINATION APPROVAL AND VECTORS FOR THE REALIZATION OF THE SAME
DE69840382D1 (en) 1997-03-18 2009-02-05 Novozymes As METHOD FOR THE PRODUCTION OF A LIBRARY THROUGH DNA SHUFFLING
DK1015575T3 (en) 1997-03-18 2010-08-23 Novozymes As Shuffling of heterologous DNA sequences
US5948653A (en) 1997-03-21 1999-09-07 Pati; Sushma Sequence alterations using homologous recombination
US6153410A (en) 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
AU746786B2 (en) 1997-12-08 2002-05-02 California Institute Of Technology Method for creating polynucleotide and polypeptide sequences
US6087341A (en) 1998-02-12 2000-07-11 The Board Of Trustees Of The Leland Standford Junior University Introduction of nucleic acid into skin cells by topical application
US6365408B1 (en) 1998-06-19 2002-04-02 Maxygen, Inc. Methods of evolving a polynucleotides by mutagenesis and recombination

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