CA2318542A1 - Encapsulated ipg strips - Google Patents

Encapsulated ipg strips Download PDF

Info

Publication number
CA2318542A1
CA2318542A1 CA002318542A CA2318542A CA2318542A1 CA 2318542 A1 CA2318542 A1 CA 2318542A1 CA 002318542 A CA002318542 A CA 002318542A CA 2318542 A CA2318542 A CA 2318542A CA 2318542 A1 CA2318542 A1 CA 2318542A1
Authority
CA
Canada
Prior art keywords
strip
encapsulated
apertures
ipg
enclosure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002318542A
Other languages
French (fr)
Other versions
CA2318542C (en
Inventor
Adriana J. Harbers
Lee Olech
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bio Rad Laboratories Inc
Original Assignee
Adriana J. Harbers
Lee Olech
Bio-Rad Laboratories, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Adriana J. Harbers, Lee Olech, Bio-Rad Laboratories, Inc. filed Critical Adriana J. Harbers
Publication of CA2318542A1 publication Critical patent/CA2318542A1/en
Application granted granted Critical
Publication of CA2318542C publication Critical patent/CA2318542C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture

Abstract

Immobilized pH gradient (IPG) strips are encapsulated in an enclosure that seals the strip (12) from atmospheric exposure and yet contains sufficient volumetric capacity (15) to accommodate the strip in rehydrated form. The enclosure contains a pair of electrode access apertures (21, 22) each of which is spanned by a dialysis membrane to permit electrical contact between external electrodes and the encapsulated IPG strip, and one or more sample application apertures (23) through which lipid sample can be applied to the strip. Each of the apertures is covered by a protective cover or flap (24, 25, 26) that is removable to expose the aperture.
CA002318542A 1998-11-19 1999-10-20 Encapsulated ipg strips Expired - Fee Related CA2318542C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/195,983 US6156182A (en) 1998-11-19 1998-11-19 Encapsulated IPG Strips
US09/195,983 1998-11-19
PCT/US1999/024628 WO2000031526A1 (en) 1998-11-19 1999-10-20 Encapsulated ipg strips

Publications (2)

Publication Number Publication Date
CA2318542A1 true CA2318542A1 (en) 2000-06-02
CA2318542C CA2318542C (en) 2003-08-05

Family

ID=22723634

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002318542A Expired - Fee Related CA2318542C (en) 1998-11-19 1999-10-20 Encapsulated ipg strips

Country Status (7)

Country Link
US (1) US6156182A (en)
EP (1) EP1047933B1 (en)
JP (1) JP3382225B2 (en)
AU (1) AU762384B2 (en)
CA (1) CA2318542C (en)
DE (1) DE69926725T2 (en)
WO (1) WO2000031526A1 (en)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19809436A1 (en) * 1998-03-05 1999-09-09 Braun Gmbh Attachment for a device for plucking hair from human skin
US7517442B1 (en) * 1999-08-09 2009-04-14 Life Technologies Corporation Facile method and apparatus for the analysis of biological macromolecules in two dimensions using common and familiar electrophoresis formats
ATE445454T1 (en) * 2001-05-10 2009-10-15 Life Technologies Corp METHOD AND DEVICES FOR ELECTROPHORESIS OF PRE-POURED, HYDRATIZABLE SEPARATION MEDIA
US7601251B2 (en) * 2001-05-10 2009-10-13 Life Technologies Corporation Methods and apparatus for low resistance electrophoresis of prior-cast, hydratable separation media
DK1410029T3 (en) 2001-07-16 2008-01-07 Protein Forest Inc Arrays of buffers for analysis of biomolecules at their isoelectric point
AU2003202918A1 (en) * 2002-01-07 2003-07-24 Proteologics, Inc. Methods and compositions for isoelectric focusing
US6655649B2 (en) 2002-03-11 2003-12-02 Bio-Rad Laboratories, Inc. Assembly for casting and use of an isoelectric focusing strip
US7153405B2 (en) * 2002-04-12 2006-12-26 Tecan Trading Ag Cassette, system, and 2-D gel electrophoresis method for separating molecules
DE50302642D1 (en) * 2002-04-12 2006-05-11 Tecan Trading Ag Cassette, system and 2D gel electrophoresis method for separating molecules
EP1546359A4 (en) 2002-09-12 2007-05-09 Molecular Probes Inc Site-specific labeling of affinity tags in fusion proteins
US7622028B2 (en) * 2003-05-09 2009-11-24 Life Technologies Corporation Solution phase electrophoresis device, components, and methods
WO2004104548A2 (en) * 2003-05-09 2004-12-02 Invitrogen Corporation Solution phase electrophoresis device, components, and methods
WO2004113899A1 (en) * 2003-06-17 2004-12-29 Invitrogen Corporation Chamber-forming electrophoresis cassette cover
US20050011762A1 (en) * 2003-06-26 2005-01-20 Provonchee Richard B. Bottom access electrophoresis tray and method of use
US7410560B2 (en) * 2003-09-11 2008-08-12 Bio-Rad Laboratories, Inc. Device for storage and delivery of standards for gel electrophoresis
WO2005029055A1 (en) * 2003-09-19 2005-03-31 Invitrogen Corporation Composite compositions for electrophoresis
WO2005029060A1 (en) 2003-09-24 2005-03-31 Agilent Technologies, Inc. Electrophoretic separation of amphoteric molecules
EP1668146B1 (en) * 2003-09-25 2014-11-12 Life Technologies Corporation Homogeneous populations of molecules
US20050103629A1 (en) * 2003-10-07 2005-05-19 Tom Diller Isoelectric focusing gels and methods of use thereof
SE0303581D0 (en) * 2003-12-23 2003-12-23 Amersham Biosciences Ab Novel use of positively charged support
EP2069386A4 (en) 2006-07-21 2009-10-28 Life Technologies Corp Sharply resolving labeled protein molecular weight standards
US20110100821A1 (en) * 2007-04-20 2011-05-05 Brian Furmanski Mineral oil free isoelectric focusing apparatus for immobilized ph gradient strips
CN104788536A (en) * 2015-04-28 2015-07-22 扬州大学 Testing device for isoelectric focusing separation of amphoteric electrolyte and application method of testing device

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3888758A (en) * 1973-06-28 1975-06-10 Sheik Arshad Saeed Apparatus for large scale gel electrophoresis
DE2908044C2 (en) * 1978-03-10 1983-03-03 Jenoptik Jena Gmbh, Ddr 6900 Jena Arrangement for cell electrophoresis
US4443319A (en) * 1982-09-30 1984-04-17 E. I. Du Pont De Nemours And Company Device for electrophoresis
US5209831A (en) * 1991-06-14 1993-05-11 Macconnell William P Bufferless electrophoresis system and method
US5443704A (en) * 1991-12-31 1995-08-22 Fmc Corporation Electrophoresis gel container assemblies
US5627022A (en) * 1994-11-01 1997-05-06 Visible Genetics Inc. Microgels for use in medical diagnosis and holders useful in fabricating same
US5582702A (en) * 1995-04-26 1996-12-10 Ethrog Biotechnology Ltd. Apparatus and method for electrophoresis
US5773645A (en) * 1997-05-05 1998-06-30 Bio-Rad Laboratories, Inc. Two-dimensional electrophoresis device
US5989400A (en) * 1997-06-09 1999-11-23 Hoefer Pharmacia Biotech, Inc. Device and method for applying power to gel electrophoresis modules
US5993627A (en) * 1997-06-24 1999-11-30 Large Scale Biology Corporation Automated system for two-dimensional electrophoresis
AUPP108697A0 (en) * 1997-12-23 1998-01-22 Macquarie Research Limited Apparatus for electrophoresis

Also Published As

Publication number Publication date
WO2000031526A1 (en) 2000-06-02
DE69926725D1 (en) 2005-09-22
JP3382225B2 (en) 2003-03-04
AU762384B2 (en) 2003-06-26
EP1047933A1 (en) 2000-11-02
DE69926725T2 (en) 2006-06-29
AU1127900A (en) 2000-06-13
JP2002530673A (en) 2002-09-17
US6156182A (en) 2000-12-05
EP1047933B1 (en) 2005-08-17
CA2318542C (en) 2003-08-05

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