CA2341143A1 - Production of motif-specific and context-independent antibodies using peptide libraries as antigens - Google Patents

Production of motif-specific and context-independent antibodies using peptide libraries as antigens Download PDF

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Publication number
CA2341143A1
CA2341143A1 CA002341143A CA2341143A CA2341143A1 CA 2341143 A1 CA2341143 A1 CA 2341143A1 CA 002341143 A CA002341143 A CA 002341143A CA 2341143 A CA2341143 A CA 2341143A CA 2341143 A1 CA2341143 A1 CA 2341143A1
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CA
Canada
Prior art keywords
context
specific
motif
amino acid
recognition sites
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002341143A
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French (fr)
Other versions
CA2341143C (en
Inventor
Yi Tan
Michael J. Comb
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cell Signaling Technology Inc
Original Assignee
Cell Signaling Technology
Yi Tan
Michael J. Comb
New England Biolabs, Inc.
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Publication date
Application filed by Cell Signaling Technology, Yi Tan, Michael J. Comb, New England Biolabs, Inc. filed Critical Cell Signaling Technology
Publication of CA2341143A1 publication Critical patent/CA2341143A1/en
Application granted granted Critical
Publication of CA2341143C publication Critical patent/CA2341143C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/44—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids

Abstract

A method is provided for producing motif-specific, context-independent antibodies which are specific to at least one fixed amino acid residue whether modified or unmodified, in the context of variable surrounding amino acid or peptide sequences. The method includes the steps of (1) constructing a peptide library featuring at least one fixed amino acid and variable surrounding amino acids, and (2) immunizing a host with this peptide library. Antibodies may optionally be isolated and purified from the antisera of this immunized host. The disclosed method encompasses both modified motifs, such as phosphothreonine, phosphoserine, MAPK consensus recognition sites, 14-3-3 consensus recognition sites, CDK consensus recognition sites, and acetylated-lysine, as well as unmodified motifs.
CA002341143A 1998-09-04 1999-08-26 Production of motif-specific and context-independent antibodies using peptide libraries as antigens Expired - Lifetime CA2341143C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/148,712 US6441140B1 (en) 1998-09-04 1998-09-04 Production of motif-specific and context-independent antibodies using peptide libraries as antigens
US09/148,712 1998-09-04
PCT/US1999/019597 WO2000014536A1 (en) 1998-09-04 1999-08-26 Production of motif-specific and context-independent antibodies using peptide libraries as antigens

Publications (2)

Publication Number Publication Date
CA2341143A1 true CA2341143A1 (en) 2000-03-16
CA2341143C CA2341143C (en) 2006-11-21

Family

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Family Applications (1)

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CA002341143A Expired - Lifetime CA2341143C (en) 1998-09-04 1999-08-26 Production of motif-specific and context-independent antibodies using peptide libraries as antigens

Country Status (6)

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US (5) US6441140B1 (en)
EP (3) EP1110086B1 (en)
JP (5) JP3732408B2 (en)
CA (1) CA2341143C (en)
DE (1) DE69936389T2 (en)
WO (1) WO2000014536A1 (en)

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Also Published As

Publication number Publication date
DE69936389T2 (en) 2008-02-28
JP2006036784A (en) 2006-02-09
WO2000014536A1 (en) 2000-03-16
EP2325204A2 (en) 2011-05-25
EP1110086B1 (en) 2007-06-27
EP1850131A1 (en) 2007-10-31
US7344714B2 (en) 2008-03-18
DE69936389D1 (en) 2007-08-09
US6982318B1 (en) 2006-01-03
EP2325204B1 (en) 2014-08-13
US20020132988A1 (en) 2002-09-19
EP1110086A1 (en) 2001-06-27
JP3732408B2 (en) 2006-01-05
US7259022B2 (en) 2007-08-21
JP2002524092A (en) 2002-08-06
US20020168684A1 (en) 2002-11-14
JP2016210783A (en) 2016-12-15
US6441140B1 (en) 2002-08-27
EP1110086A4 (en) 2003-08-27
CA2341143C (en) 2006-11-21
JP5648007B2 (en) 2015-01-07
US20030068652A1 (en) 2003-04-10
JP5013693B2 (en) 2012-08-29
JP2014132019A (en) 2014-07-17
EP2325204A3 (en) 2011-10-26
JP2012162535A (en) 2012-08-30
EP1850131B1 (en) 2013-01-23

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