CA2380616A1 - Process for the biological production of 1,3-propanediol with high titer - Google Patents
Process for the biological production of 1,3-propanediol with high titer Download PDFInfo
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- CA2380616A1 CA2380616A1 CA002380616A CA2380616A CA2380616A1 CA 2380616 A1 CA2380616 A1 CA 2380616A1 CA 002380616 A CA002380616 A CA 002380616A CA 2380616 A CA2380616 A CA 2380616A CA 2380616 A1 CA2380616 A1 CA 2380616A1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
Abstract
The present invention provides an improved method for the biological production of 1,3-propanediol from a fermentable carbon source in a single microorganism. In one aspect of the present invention, an improved process for the conversion of glucose to 1,3-propanediol is achieved by the use of an E.
coli transformed with the Klebsiella pneumoniae dha regulon genes dhaR, orfY, dhaT, orfX, orfW, dhaB1, dhaB2, dhaB3, and orfZ, all these genes arranged in the same genetic organization as found in wild type Klebsiella pneumoniae. In another aspect of the present invention, an improved process for the production of 1,3-propanediol from glucose using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, and a dehydratase reactivation factor compared to an identical process using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, a dehydratase reactivation factor and a 1,3-propanediol oxidoreductase (dhaT). The dramatically improved process relies on the presence in E. Coli of a gene encoding a non-specific catalytic activity sufficient to convert 3-hydroxypropionaldehyde to 1,3-propanediol.
coli transformed with the Klebsiella pneumoniae dha regulon genes dhaR, orfY, dhaT, orfX, orfW, dhaB1, dhaB2, dhaB3, and orfZ, all these genes arranged in the same genetic organization as found in wild type Klebsiella pneumoniae. In another aspect of the present invention, an improved process for the production of 1,3-propanediol from glucose using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, and a dehydratase reactivation factor compared to an identical process using a recombinant E. coli containing genes encoding a G3PDH, a G3P phosphatase, a dehydratase, a dehydratase reactivation factor and a 1,3-propanediol oxidoreductase (dhaT). The dramatically improved process relies on the presence in E. Coli of a gene encoding a non-specific catalytic activity sufficient to convert 3-hydroxypropionaldehyde to 1,3-propanediol.
Claims (29)
1. An isolated nucleic acid fragment encoding a non-specific catalytic activity for the conversion of 3-hydroxypropionaldehyde to 1,3-propanediol and selected from the group consisting of:
(a) an isolated nucleic acid fragment encoding all or a substantial portion of the amino acid sequence of SEQ ID NO:57;
(e) an isolated nucleic acid fragment that is substantially similar to an isolated nucleic acid fragment encoding all or a substantial portion of the amino acid sequence of SEQ ID NO:57;
(f) an isolated nucleic acid fragment encoding a polypeptide of at least 387 amino acids having at least 80% with the amino acid sequence of SEQ ID NO:57;
(d) an isolated nucleic acid fragment that hybridizes with (a) under hybridization conditions of 0.1X SSC, 0.1% SDS, 65 °C and washed with 2X SSC, 0.1% SDS followed by 0.1X SSC, 0.1%
SDS; and (e) an isolated nucleic acid fragment that is complementary to (a), (b), (c), or (d).
(a) an isolated nucleic acid fragment encoding all or a substantial portion of the amino acid sequence of SEQ ID NO:57;
(e) an isolated nucleic acid fragment that is substantially similar to an isolated nucleic acid fragment encoding all or a substantial portion of the amino acid sequence of SEQ ID NO:57;
(f) an isolated nucleic acid fragment encoding a polypeptide of at least 387 amino acids having at least 80% with the amino acid sequence of SEQ ID NO:57;
(d) an isolated nucleic acid fragment that hybridizes with (a) under hybridization conditions of 0.1X SSC, 0.1% SDS, 65 °C and washed with 2X SSC, 0.1% SDS followed by 0.1X SSC, 0.1%
SDS; and (e) an isolated nucleic acid fragment that is complementary to (a), (b), (c), or (d).
2. The isolated nucleic acid fragment as set out in SEQ ID NO:58.
3. A polypeptide encoded by the isolated nucleic acid fragment of Claim 1.
4. The polypeptide of Claim 3 as set out in SEQ ID NO:57.
5. A chimeric gene comprising the isolated nucleic acid fragment of Claim 1 operably linked to suitable regulatory sequences.
6. A microorganism transformed with the chimeric gene of Claim 5 wherein the microorganism is selected from the group consisting of Citrobacter, Enterobacter, Clostridium, Klebsiella, Aerobacter, Lactobacillus, Aspergillus, Saccharomyces, Schizosaccharomyces, Zygosaccharomyces, Pichia, Kluyveromyces, Candida, Hansenula, Debaryomyces, Mucor, Torulopsis, Methylobacter, Salmonella, Bacillus, Aerobacter, Streptomyces and Pseudomonas.
7. A recombinant microorganism, useful for the production of 1,3-propanediol, comprising:
(a) at least one gene encoding a polypeptide having a dehydratase activity;
(b) at least one gene encoding a dehydratase reactivation factor;
and (c) at least one exogenous gene encoding an non-specific catalytic activity to convert 3-hydroxypropionaldehyde to 1,3-propanediol;
wherein no functional dhaT gene encoding a 1,3-propanediol oxidoreductase activity is present in the recombinant microorganism and the microorganism is selected from the group consisting of Citrobacter, Enterobacter, Clostridium, Klebsiella, Aerobacter, Lactobacillus, Aspergillus, Saccharomyces, Schizosaccharomyces, Zygosaccharomyces, Pichia, Kluyveromyces, Candida, Hansenula, Debaryomyces, Mucor, Torulopsis, Methylobacter, Salmonella, Bacillus, Aerobacter, Streptomyces and Pseudomonas.
(a) at least one gene encoding a polypeptide having a dehydratase activity;
(b) at least one gene encoding a dehydratase reactivation factor;
and (c) at least one exogenous gene encoding an non-specific catalytic activity to convert 3-hydroxypropionaldehyde to 1,3-propanediol;
wherein no functional dhaT gene encoding a 1,3-propanediol oxidoreductase activity is present in the recombinant microorganism and the microorganism is selected from the group consisting of Citrobacter, Enterobacter, Clostridium, Klebsiella, Aerobacter, Lactobacillus, Aspergillus, Saccharomyces, Schizosaccharomyces, Zygosaccharomyces, Pichia, Kluyveromyces, Candida, Hansenula, Debaryomyces, Mucor, Torulopsis, Methylobacter, Salmonella, Bacillus, Aerobacter, Streptomyces and Pseudomonas.
8. The recombinant microorganism of Claim 7 further comprising:
(a) at least one gene encoding a polypeptide having glycerol-3-phosphate dehydrogenase activity; and (b) at least one gene encoding a polypeptide having glycerol-3-phosphatase activity;
(a) at least one gene encoding a polypeptide having glycerol-3-phosphate dehydrogenase activity; and (b) at least one gene encoding a polypeptide having glycerol-3-phosphatase activity;
9. The recombinant microorganism of Claim 7 or 8 wherein the dehydratase reactivation factor is encoded by orfX and orfZ, isolated from a dha regulon.
10. The recombinant microorganism of Claim 9 wherein orfX and orfZ are independently isolated from Klebsiella sp., Citrobacter sp., or Clostridium sp.
11. The recombinant microorganism of Claim 8, further comprising a set of endogenous genes, each having a mutation inactivating the gene, the set consisting of:
(a) a first gene encoding a polypeptide having glycerol kinase activity;
(b) a second gene encoding a polypeptide having glycerol dehydrogenase activity; and (c) a third gene encoding a polypeptide having triosephosphate isomerase activity.
(a) a first gene encoding a polypeptide having glycerol kinase activity;
(b) a second gene encoding a polypeptide having glycerol dehydrogenase activity; and (c) a third gene encoding a polypeptide having triosephosphate isomerase activity.
12. The recombinant microorganism of Claims 8 or 11 wherein the recombinant microorganism converts a carbon source selected from the group consisting of monosaccharides, oligosaccharides, polysaccharides, and single-carbon substrates to 1,3-propanediol.
13. The recombinant microorganism of Claim 7 wherein the recombinant microorganism converts a carbon source selected from the group consisting of glycerol and dihydroxyacetone to 1,3-propanediol.
14. The recombinant microorganism of Claims 8 or 11 wherein the gene encoding a polypeptide having glycerol-3-phosphate dehydrogenase activity is selected from the group consisting of GPD1, GPD2, GPD3, DAR1, gpsA, GUT2, glpD, and glpABC.
15. The recombinant microorganism of Claims 8 or 11 wherein the gene encoding a polypeptide having glycerol-3-phosphatase activity is selected from the group consisting of GPP1 and GPP2.
16. The recombinant microorganism of Claims 7, 8 or 11 wherein the gene encoding a polypeptide having a dehydratase activity is selected from the group consisting of a glycerol dehydratase and a diol dehydratase.
17. The recombinant microorganism of Claims 7, 8 and 11 wherein the gene encoding a polypeptide having a dehydratase activity is isolated from Klebsiella sp., Citrobacter sp., or Clostridium sp.
18. A recombinant E. coli comprising:
(a) a set of exogenous genes consisting of:
(i) at least one gene encoding a polypeptide having a dehydratase activity;
(ii) at least one gene encoding a polypeptide having glycerol-3-phosphate dehydrogenase activity;
(iii) at least one gene encoding a polypeptide having glycerol-3-phosphatase activity; and (iv) at least one gene encoding a dehydratase reactivation factor; and (b) at least one endogenous gene encoding a non-specific catalytic activity to convert 3-hydroxypropionaldehyde to 1,3-propanediol;
wherein no functional dhaT gene encoding a 1,3-propanediol oxidoreductase activity is present in the recombinant E. coli.
(a) a set of exogenous genes consisting of:
(i) at least one gene encoding a polypeptide having a dehydratase activity;
(ii) at least one gene encoding a polypeptide having glycerol-3-phosphate dehydrogenase activity;
(iii) at least one gene encoding a polypeptide having glycerol-3-phosphatase activity; and (iv) at least one gene encoding a dehydratase reactivation factor; and (b) at least one endogenous gene encoding a non-specific catalytic activity to convert 3-hydroxypropionaldehyde to 1,3-propanediol;
wherein no functional dhaT gene encoding a 1,3-propanediol oxidoreductase activity is present in the recombinant E. coli.
19. A recombinant E. coli comprising:
(a) a set of exogenous genes consisting of (i) at least one gene encoding a polypeptide having glycerol-3-phosphate dehydrogenase activity;
(ii) at least one gene encoding a polypeptide having glycerol-3-phosphatase activity; and (iii) at least one subset of genes encoding the gene products of dhaR, orfY, orfX, orfW, dhaB1, dhaB2, dhaB3 and orfZ, and (b) at least one endogenous gene encoding a non-specific catalytic activity to convert 3-hydroxypropionaldehyde to 1,3-propanediol, wherein no functional dhaT gene encoding a 1,3-propanediol oxidoreductase activity is present in the recombinant E. coli.
(a) a set of exogenous genes consisting of (i) at least one gene encoding a polypeptide having glycerol-3-phosphate dehydrogenase activity;
(ii) at least one gene encoding a polypeptide having glycerol-3-phosphatase activity; and (iii) at least one subset of genes encoding the gene products of dhaR, orfY, orfX, orfW, dhaB1, dhaB2, dhaB3 and orfZ, and (b) at least one endogenous gene encoding a non-specific catalytic activity to convert 3-hydroxypropionaldehyde to 1,3-propanediol, wherein no functional dhaT gene encoding a 1,3-propanediol oxidoreductase activity is present in the recombinant E. coli.
20. The recombinant E. coli of Claim 19 further comprising a set of endogenous genes, each gene having a mutation inactivating the gene, the set consisting of:
(a) a gene encoding a polypeptide having glycerol kinase activity;
(b) a gene encoding a polypeptide having glycerol dehydrogenase activity; and (c) a gene encoding a polypeptide having triosephosphate isomerase activity.
(a) a gene encoding a polypeptide having glycerol kinase activity;
(b) a gene encoding a polypeptide having glycerol dehydrogenase activity; and (c) a gene encoding a polypeptide having triosephosphate isomerase activity.
21. A process for the bioproduction of 1,3-propanediol comprising:
(a) contacting under suitable conditions the recombinant E. coli of either Claim 19 or Claim 20 with at least one carbon source selected from the group consisting of monosaccharides, oligosaccharides, polysaccharides, and single-carbon substrates whereby 1,3-propanediol is produced; and (b) optionally recovering the 1,3-propanediol produced in (a).
(a) contacting under suitable conditions the recombinant E. coli of either Claim 19 or Claim 20 with at least one carbon source selected from the group consisting of monosaccharides, oligosaccharides, polysaccharides, and single-carbon substrates whereby 1,3-propanediol is produced; and (b) optionally recovering the 1,3-propanediol produced in (a).
22. A process for the bioproduction of 1,3-propanediol comprising:
(a) contacting the recombinant E. coli of Claims 19 or 20 or the recombinant E.coli of Claims 19 or 20 further comprising:
(i) at least one exogenous gene encoding a polypeptide having a dehydratase activity;
(ii) at least one exogenous gene encoding a dehydratase reactivation factor;
(iii) at least one endogenous gene encoding a non-specific catalytic activity to convert 3-hydroxy-propionaldehyde to 1,3-propanediol, with at least one carbon source selected from the group consisting of glycerol and dihydroxyacetone, and (b) optionally recovering the 1,3-propanediol produced in (a).
(a) contacting the recombinant E. coli of Claims 19 or 20 or the recombinant E.coli of Claims 19 or 20 further comprising:
(i) at least one exogenous gene encoding a polypeptide having a dehydratase activity;
(ii) at least one exogenous gene encoding a dehydratase reactivation factor;
(iii) at least one endogenous gene encoding a non-specific catalytic activity to convert 3-hydroxy-propionaldehyde to 1,3-propanediol, with at least one carbon source selected from the group consisting of glycerol and dihydroxyacetone, and (b) optionally recovering the 1,3-propanediol produced in (a).
23. A process for the production of 1,3-propanediol comprising:
(a) contacting a recombinant E. coli with a first source of carbon and with a second source of carbon, the recombinant E. coli comprising:
(i) at least one exogenous gene encoding a polypeptide having a dehydratase activity;
(ii) at least one exogenous gene encoding a dehydratase reactivation factor;
(iii) at least one endogenous gene encoding a non-specific catalytic activity sufficient to convert 3-hydroxypropionaldehyde to 1,3-propanediol, wherein no functional dhaT gene encoding a 1,3-propanediol oxidoreductase activity is present in the recombinant E. coli and wherein the first carbon source is selected from the group consisting of glycerol and dihydroxyacetone, and the second carbon source is selected from the group consisting of monosaccharides, oligosaccharides, polysaccharides, and single-carbon substrates; and (b) optionally recovering the 1,3-propanediol produced in (a).
(a) contacting a recombinant E. coli with a first source of carbon and with a second source of carbon, the recombinant E. coli comprising:
(i) at least one exogenous gene encoding a polypeptide having a dehydratase activity;
(ii) at least one exogenous gene encoding a dehydratase reactivation factor;
(iii) at least one endogenous gene encoding a non-specific catalytic activity sufficient to convert 3-hydroxypropionaldehyde to 1,3-propanediol, wherein no functional dhaT gene encoding a 1,3-propanediol oxidoreductase activity is present in the recombinant E. coli and wherein the first carbon source is selected from the group consisting of glycerol and dihydroxyacetone, and the second carbon source is selected from the group consisting of monosaccharides, oligosaccharides, polysaccharides, and single-carbon substrates; and (b) optionally recovering the 1,3-propanediol produced in (a).
24. The process of Claim 23 wherein the recombinant E. coli further comprises (a) a set of exogenous genes consisting of (i) at least one gene encoding a polypeptide having glycerol-3-phosphate dehydrogenase activity;
(ii) at least one gene encoding a polypeptide having glycerol-3-phosphatase activity; and (iii) at least one subset of genes encoding the gene products of dhaR, orfY, orfX, orfW, dhaB1, dhaB2, dhaB3 and orfZ, and (b) a set of endogenous genes, each gene having a mutation inactivating the gene, the set consisting of:
(i) a gene encoding a polypeptide having glycerol kinase activity;
(ii) a gene encoding a polypeptide having glycerol dehydrogenase activity; and (iii) a gene encoding a polypeptide having triosephosphate isomerase activity.
(ii) at least one gene encoding a polypeptide having glycerol-3-phosphatase activity; and (iii) at least one subset of genes encoding the gene products of dhaR, orfY, orfX, orfW, dhaB1, dhaB2, dhaB3 and orfZ, and (b) a set of endogenous genes, each gene having a mutation inactivating the gene, the set consisting of:
(i) a gene encoding a polypeptide having glycerol kinase activity;
(ii) a gene encoding a polypeptide having glycerol dehydrogenase activity; and (iii) a gene encoding a polypeptide having triosephosphate isomerase activity.
25. Vector pDT29 comprising a set of genes dhaR, orfY, dhaT, orfX, orfW, dhaB1, dhaB2, dhaB3, and orfZ as set forth in SEQ ID NO:1.
26. Vector pKP32 comprising dhaR, orfY, orfX, orfW, dhaB1, dhaB2, dhaB3 and orfZ as set forth in SEQ ID NO: 1.
27. A recombinant E. coli strain KLP23 comprising:
(a) a set of two endogenous genes, each gene having a mutation inactivating the gene, the set consisting of (i) a gene encoding a polypeptide having a glycerol kinase activity; and (ii) a gene encoding a polypeptide having a glycerol dehydrogenase activity;
(b) at least one exogenous gene encoding a polypeptide having glycerol-3-phosphate dehydrogenase activity;
(c) at least one exogenous gene encoding a polypeptide having glycerol-3-phosphatase activity; and (d) a plasmid pKP32.
(a) a set of two endogenous genes, each gene having a mutation inactivating the gene, the set consisting of (i) a gene encoding a polypeptide having a glycerol kinase activity; and (ii) a gene encoding a polypeptide having a glycerol dehydrogenase activity;
(b) at least one exogenous gene encoding a polypeptide having glycerol-3-phosphate dehydrogenase activity;
(c) at least one exogenous gene encoding a polypeptide having glycerol-3-phosphatase activity; and (d) a plasmid pKP32.
28. A recombinant E. coli strain RJ8 comprising:
(a) set of three endogenous genes, each gene having a mutation inactivating the gene, the set consisting of:
(i) a gene encoding a polypeptide having a glycerol kinase activity;
(ii) a gene encoding a polypeptide having a glycerol dehydrogenase activity; and (iii) a gene encoding a polypeptide having a triosephosphate isomerase activity.
(a) set of three endogenous genes, each gene having a mutation inactivating the gene, the set consisting of:
(i) a gene encoding a polypeptide having a glycerol kinase activity;
(ii) a gene encoding a polypeptide having a glycerol dehydrogenase activity; and (iii) a gene encoding a polypeptide having a triosephosphate isomerase activity.
29. A process for the production of 1,3-propanediol comprising:
(a) contacting, under suitable conditions, a recombinant E. coli comprising a dha regulon and lacking a functional dhaT gene encoding a 1,3-propanediol oxidoreductase activity with at least one carbon source, wherein the carbon source is selected from the group consisting of monosaccharides, oligosaccharides, polysaccharides, and single-carbon substrates; and (b) optionally recovering the 1,3-propanediol produced in (a).
(a) contacting, under suitable conditions, a recombinant E. coli comprising a dha regulon and lacking a functional dhaT gene encoding a 1,3-propanediol oxidoreductase activity with at least one carbon source, wherein the carbon source is selected from the group consisting of monosaccharides, oligosaccharides, polysaccharides, and single-carbon substrates; and (b) optionally recovering the 1,3-propanediol produced in (a).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA2733616A CA2733616C (en) | 1999-08-18 | 2000-08-18 | Process for the biological production of 1,3-propanediol with high titer |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US14953499P | 1999-08-18 | 1999-08-18 | |
| US60/149,534 | 1999-08-18 | ||
| PCT/US2000/022874 WO2001012833A2 (en) | 1999-08-18 | 2000-08-18 | Process for the biological production of 1,3-propanediol |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2733616A Division CA2733616C (en) | 1999-08-18 | 2000-08-18 | Process for the biological production of 1,3-propanediol with high titer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2380616A1 true CA2380616A1 (en) | 2001-02-22 |
| CA2380616C CA2380616C (en) | 2011-05-24 |
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| CA2380616A Expired - Lifetime CA2380616C (en) | 1999-08-18 | 2000-08-18 | Process for the biological production of 1,3-propanediol with high titer |
| CA2733616A Expired - Lifetime CA2733616C (en) | 1999-08-18 | 2000-08-18 | Process for the biological production of 1,3-propanediol with high titer |
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| CA2733616A Expired - Lifetime CA2733616C (en) | 1999-08-18 | 2000-08-18 | Process for the biological production of 1,3-propanediol with high titer |
Country Status (13)
| Country | Link |
|---|---|
| US (5) | US6514733B1 (en) |
| EP (1) | EP1204755B1 (en) |
| JP (1) | JP4716634B2 (en) |
| KR (1) | KR100785997B1 (en) |
| CN (2) | CN101024843A (en) |
| AT (1) | ATE335823T1 (en) |
| BR (2) | BR0013315B1 (en) |
| CA (2) | CA2380616C (en) |
| DE (1) | DE60029971T2 (en) |
| DK (1) | DK1204755T3 (en) |
| HK (2) | HK1044963B (en) |
| PT (1) | PT1204755E (en) |
| WO (1) | WO2001012833A2 (en) |
Families Citing this family (120)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69831813T2 (en) | 1997-12-02 | 2006-07-20 | E.I. Dupont De Nemours And Co., Wilmington | METHOD OF GENERATING GLYCERINE BY RECOMOMBINANT ORGANISMS |
| CA2443145C (en) | 2001-04-04 | 2013-01-22 | Genencor International, Inc. | Uncoupled productive and catabolic host cell pathways |
| EP2039774A1 (en) | 2001-04-04 | 2009-03-25 | Genencor International, Inc. | Methods for the production of products in host cells |
| JP2002330763A (en) * | 2001-05-02 | 2002-11-19 | Ajinomoto Co Inc | Method for producing objective substance by fermentation |
| JP2005523020A (en) * | 2002-04-22 | 2005-08-04 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー | Promoter and plasmid systems for genetic engineering |
| US20050079617A1 (en) | 2003-10-03 | 2005-04-14 | Cervin Marguerite A. | Glucose transport mutants for production of biomaterial |
| CN100471946C (en) | 2002-10-04 | 2009-03-25 | 纳幕尔杜邦公司 | Process for the biological production of 1,3-propanediol with high yield |
| EP1546304B1 (en) | 2002-10-04 | 2013-05-08 | Danisco US Inc. | Improved production of bacterial strains |
| CN102031265A (en) | 2002-12-16 | 2011-04-27 | 纳幕尔杜邦公司 | B-12 dependent dehydratases with improved reaction kinetics |
| KR100864672B1 (en) * | 2003-04-02 | 2008-10-23 | 씨제이제일제당 (주) | A method for producing a 1,2-propanediol using Klebsiella pneumoniae |
| US7323539B2 (en) | 2003-05-06 | 2008-01-29 | E. I. Du Pont De Nemours And Company | Polytrimethylene ether glycol and polytrimethylene ether ester with excellent quality |
| US7056439B2 (en) * | 2003-05-06 | 2006-06-06 | Tate & Lyle Ingredidents Americas, Inc. | Process for producing 1, 3-propanediol |
| FR2864967B1 (en) | 2004-01-12 | 2006-05-19 | Metabolic Explorer Sa | ADVANCED MICROORGANISM FOR THE PRODUCTION OF 1,2-PROPANEDIOL |
| US20070148749A1 (en) * | 2004-03-26 | 2007-06-28 | Shinzo Yasuda | Process for producting 1,3-propanediol and or/3-hydroxypropionic acid |
| WO2005103262A1 (en) * | 2004-04-20 | 2005-11-03 | Cj Corp. | A novel diol dehydratase gene cluster in listeria genus |
| US20070029252A1 (en) * | 2005-04-12 | 2007-02-08 | Dunson James B Jr | System and process for biomass treatment |
| US7781191B2 (en) * | 2005-04-12 | 2010-08-24 | E. I. Du Pont De Nemours And Company | Treatment of biomass to obtain a target chemical |
| EP1869202B1 (en) * | 2005-04-12 | 2018-02-14 | E. I. du Pont de Nemours and Company | Treatment of biomass to obtain fermentable sugars |
| CN103172840B (en) | 2005-04-22 | 2016-03-02 | 三菱化学株式会社 | From polyester and the manufacture method thereof of biomass resource |
| US7524660B2 (en) | 2005-05-05 | 2009-04-28 | E.I. Du Pont De Nemours And Company | Utilization of fructose in microbial production strains |
| CN100427605C (en) * | 2005-06-17 | 2008-10-22 | 清华大学 | Mehtod for producing 1,3-propanediol and 2,3-cis-butanediol from crude starch material |
| US9297028B2 (en) | 2005-09-29 | 2016-03-29 | Butamax Advanced Biofuels Llc | Fermentive production of four carbon alcohols |
| NZ593809A (en) | 2005-10-26 | 2013-01-25 | Butamax Tm Advanced Biofuels | Fermentive production of four carbon alcohols |
| US8945899B2 (en) | 2007-12-20 | 2015-02-03 | Butamax Advanced Biofuels Llc | Ketol-acid reductoisomerase using NADH |
| US8956850B2 (en) * | 2008-06-05 | 2015-02-17 | Butamax Advanced Biofuels Llc | Enhanced pyruvate to acetolactate conversion in yeast |
| US20080274526A1 (en) * | 2007-05-02 | 2008-11-06 | Bramucci Michael G | Method for the production of isobutanol |
| CN100392071C (en) * | 2005-12-05 | 2008-06-04 | 中国农业大学 | Engineered bacterium lacking lactic acid production path and its construction method and uses |
| US20070275139A1 (en) * | 2006-02-10 | 2007-11-29 | Melissa Joerger | Food compositions comprising renewably-based, biodegradable1,3-propanediol |
| ES2797782T3 (en) * | 2006-02-10 | 2020-12-03 | Dupont Tate & Lyle Bio Products Company Llc | Composition comprising an extract and 1,3-propanediol of biological origin as a non-irritating solvent |
| US8206970B2 (en) | 2006-05-02 | 2012-06-26 | Butamax(Tm) Advanced Biofuels Llc | Production of 2-butanol and 2-butanone employing aminobutanol phosphate phospholyase |
| US8828704B2 (en) | 2006-05-02 | 2014-09-09 | Butamax Advanced Biofuels Llc | Fermentive production of four carbon alcohols |
| US20080003653A1 (en) * | 2006-06-29 | 2008-01-03 | Wenzel J Douglas | Supplementation of ethanol fermentations and processes including supplemental components |
| WO2008052596A1 (en) * | 2006-10-31 | 2008-05-08 | Metabolic Explorer | Process for the biological production of n-butanol with high yield |
| BRPI0622099A2 (en) | 2006-10-31 | 2011-12-27 | Metabolic Explorer Sa | Process for the biological production of 1,3-propanediol from high yield glycerol |
| US20080176957A1 (en) * | 2006-11-15 | 2008-07-24 | Dupont Tate & Lyle Bio Products Company, Llc | Preservative compositions comprising renewably-based, biodegradable 1,3-propanediol |
| CN101668861A (en) | 2007-03-16 | 2010-03-10 | 基因组股份公司 | compositions and methods for the biosynthesis of 1,4-butanediol and its precursors |
| US7910342B2 (en) * | 2007-04-18 | 2011-03-22 | Butamax(Tm) Advanced Biofuels Llc | Fermentive production of isobutanol using highly active ketol-acid reductoisomerase enzymes |
| US8426174B2 (en) * | 2007-05-02 | 2013-04-23 | Butamax(Tm) Advanced Biofuels Llc | Method for the production of 2-butanol |
| DE102007027006A1 (en) * | 2007-06-08 | 2008-12-11 | Evonik Degussa Gmbh | Microbiological production of aldehydes, in particular of 3-hydroxypropionaldehyde |
| CN101130782B (en) * | 2007-07-23 | 2011-01-26 | 江南大学 | Construction method for producing 1,3-trimethylene glycol regrouping saccharomyces cerevisiae with glucose as substrate |
| US7947483B2 (en) | 2007-08-10 | 2011-05-24 | Genomatica, Inc. | Methods and organisms for the growth-coupled production of 1,4-butanediol |
| US8445236B2 (en) * | 2007-08-22 | 2013-05-21 | Alliance For Sustainable Energy Llc | Biomass pretreatment |
| US20090053800A1 (en) * | 2007-08-22 | 2009-02-26 | Julie Friend | Biomass Treatment Apparatus |
| US7819976B2 (en) * | 2007-08-22 | 2010-10-26 | E. I. Du Pont De Nemours And Company | Biomass treatment method |
| US7807419B2 (en) * | 2007-08-22 | 2010-10-05 | E. I. Du Pont De Nemours And Company | Process for concentrated biomass saccharification |
| BRPI0816880A2 (en) | 2007-09-21 | 2015-09-08 | Basf Plant Science Gmbh | methods for producing a higher yielding plant compared with a corresponding wild-type plant, for producing a higher yielding transgenic plant compared with a corresponding unprocessed wild-type plant, for producing an agricultural composition, for identifying a plant with a increased yield, and for increasing the yield of a plant population, isolated nucleic acid molecule, nucleic acid construction, vector, processes for producing a polypeptide, and for identifying a compound, polypeptide, antibody, plant cell nucleus, cell plant material, plant tissue, propagation material, pollen, progeny, harvested material or plant, seed, plant part, transgenic plant, transgenic plant, transgenic plant cell nucleus, transgenic plant cell, plant comprising one or more of such transgenic plant cell nuclei or plant cells, prog emphasis, seed or pollen derived from or produced by a transgenic plant, composition, and use of nucleic acids. |
| AU2009291825B2 (en) | 2008-09-10 | 2016-05-05 | Genomatica, Inc. | Microorganisms for the production of 1,4-butanediol |
| EP2337869B1 (en) | 2008-09-29 | 2015-08-19 | Butamax Advanced Biofuels Llc | Enhanced pyruvate to 2,3-butanediol conversion in lactic acid bacteria |
| DE102009029567A1 (en) * | 2008-10-02 | 2010-04-08 | Basf Se | Process for depleting sulfur and / or sulfur-containing compounds from a biochemically produced organic compound |
| CN101633928B (en) * | 2008-10-22 | 2011-09-14 | 大连理工大学 | Recombinant expression of aldehyde reductase and application thereof in bioconversion of glycerol into 1,3-propylene glycol |
| WO2010064744A1 (en) * | 2008-12-03 | 2010-06-10 | Korea Research Institute Of Bioscience And Biotechnology | Mutant blocked in glycerol oxidaion pathway for producing 1,3-propanediol |
| WO2010080489A1 (en) * | 2008-12-19 | 2010-07-15 | E. I. Du Pont De Nemours And Company | Ozone treatment of biomass to enhance enzymatic saccharification |
| MX2011007039A (en) | 2008-12-31 | 2011-07-20 | Metabolic Explorer Sa | Method for the preparation of diols. |
| US8338148B2 (en) * | 2009-03-12 | 2012-12-25 | Korea Research Institute Of Bioscience And Biotechnology | Method of producing 1,3-propanediol using recombinant Klebsiella strain in which glycerol oxidative pathway has been blocked |
| KR101189187B1 (en) * | 2009-03-12 | 2012-10-10 | 한국생명공학연구원 | Method for Preparing 1,3-Propanediol Using Mutant Blocked in Glycerol Oxidation Pathway |
| SI3199511T1 (en) * | 2009-06-04 | 2020-07-31 | Genomatica, Inc. | Process of separating components of a fermentation broth |
| SI3392340T1 (en) | 2009-06-04 | 2022-05-31 | Genomatica, Inc. | Microorganisms for the production of 1,4-butanediol and related methods |
| WO2011012697A2 (en) | 2009-07-30 | 2011-02-03 | Metabolic Explorer | Mutant yqhd enzyme for the production of a biochemical by fermentation |
| KR101048485B1 (en) * | 2009-08-17 | 2011-07-12 | 부산대학교 산학협력단 | Method of culturing recombinant E. coli to simultaneously produce 3-hydroxypropionic acid and 1,3-propanediol from glycerol |
| KR20120083391A (en) | 2009-09-09 | 2012-07-25 | 유니베르시다데 에스타듀알 데 캄피나스-유니캄프 | Microorganisms and process for producing n-propanol |
| EP2488652A4 (en) * | 2009-10-13 | 2014-04-09 | Genomatica Inc | Microorganisms for the production of 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-coa, putrescine and related compounds, and methods related thereto |
| US8852903B2 (en) | 2009-10-23 | 2014-10-07 | E I Du Pont De Nemours And Company | Co-metabolism of fructose and glucose in microbial production strains |
| EP2495328B1 (en) * | 2009-10-29 | 2016-03-30 | Korea Research Institute of Bioscience and Biotechnology | Novel method for producing 3-hydroxypropionic acid from glycerol |
| US8530210B2 (en) | 2009-11-25 | 2013-09-10 | Genomatica, Inc. | Microorganisms and methods for the coproduction 1,4-butanediol and gamma-butyrolactone |
| US20110136190A1 (en) | 2009-12-04 | 2011-06-09 | E. I. Du Pont De Nemours And Company | Recombinant bacteria for producing glycerol and glycerol-derived products from sucrose |
| ES2647611T3 (en) | 2010-02-11 | 2017-12-22 | Cj Cheiljedang Corporation | Process for producing a lower alkyl acrylate and 2-butene from a genetically modified poly-3-hydroxybutyrate biomass |
| US8048661B2 (en) | 2010-02-23 | 2011-11-01 | Genomatica, Inc. | Microbial organisms comprising exogenous nucleic acids encoding reductive TCA pathway enzymes |
| US8445244B2 (en) | 2010-02-23 | 2013-05-21 | Genomatica, Inc. | Methods for increasing product yields |
| US8906235B2 (en) | 2010-04-28 | 2014-12-09 | E I Du Pont De Nemours And Company | Process for liquid/solid separation of lignocellulosic biomass hydrolysate fermentation broth |
| US8721794B2 (en) | 2010-04-28 | 2014-05-13 | E I Du Pont De Nemours And Company | Production of high solids syrup from lignocellulosic biomass hydrolysate fermentation broth |
| KR101220499B1 (en) * | 2010-05-25 | 2013-01-10 | 한국생명공학연구원 | Method for Preparing 3-Hydroxypropionic Acid from Glycerol with High Yield |
| TWI500768B (en) * | 2010-07-05 | 2015-09-21 | Metabolic Explorer Sa | Method for the preparation of 1,3-propanediol from sucrose |
| US8651403B2 (en) | 2010-07-21 | 2014-02-18 | E I Du Pont De Nemours And Company | Anhydrous ammonia treatment for improved milling of biomass |
| US20120125548A1 (en) | 2010-11-23 | 2012-05-24 | E. I. Du Pont De Nemours And Company | Continuously fed biomass pretreatment process for a packed bed reactor |
| US20120125551A1 (en) | 2010-11-23 | 2012-05-24 | E. I. Du Pont De Nemours And Company | Biomass pretreatment process for a packed bed reactor |
| US8129170B1 (en) | 2010-12-06 | 2012-03-06 | E.I. Du Pont De Nemours And Company | Recombinant bacteria having the ability to metabolize sucrose |
| CN103347999B (en) | 2010-12-13 | 2016-02-10 | 麦兰特公司 | Use containing the raw material production succsinic acid of sucrose and the method for other chemical |
| KR20140092759A (en) | 2011-03-24 | 2014-07-24 | 부타맥스 어드밴스드 바이오퓨얼스 엘엘씨 | Host cells and methods for production of isobutanol |
| JP5852732B2 (en) | 2011-04-13 | 2016-02-03 | イーエルシー マネージメント エルエルシー | Conditioning agents for personal care compositions |
| CA2830975C (en) | 2011-04-13 | 2016-06-21 | Elc Management Llc | Mild anionic surfactants suitable for personal care compositions |
| WO2012170793A1 (en) | 2011-06-08 | 2012-12-13 | Metabolix, Inc. | Biorefinery process for thf production |
| EP2540834A1 (en) * | 2011-06-29 | 2013-01-02 | Metabolic Explorer | Method for the preparation of 1,3-propanediol |
| JP2013013353A (en) * | 2011-07-01 | 2013-01-24 | Daicel Corp | Method for producing 1,3-propanediol |
| EP2742143A1 (en) | 2011-08-10 | 2014-06-18 | Metabolix, Inc. | Post process purification for gamma-butyrolactone production |
| JP2013070675A (en) * | 2011-09-28 | 2013-04-22 | Daicel Corp | Method for culturing recombinant bacterium producing alkyl diol |
| US20160024532A1 (en) * | 2012-02-23 | 2016-01-28 | The Regents Of The University Of California | Atp driven direct photosynthetic production of fuels and chemicals |
| US9017961B2 (en) | 2012-03-05 | 2015-04-28 | E.I. Du Pont De Nemours And Company | Recombinant bacteria comprising novel sucrose transporters |
| US8686114B2 (en) | 2012-03-05 | 2014-04-01 | E I Du Pont De Nemours And Company | Variant sucrose transporter polypeptides |
| KR20150014953A (en) | 2012-05-11 | 2015-02-09 | 부타맥스 어드밴스드 바이오퓨얼스 엘엘씨 | Ketol-acid reductoisomerase enzymes and methods of use |
| CN111705028A (en) | 2012-06-04 | 2020-09-25 | 基因组股份公司 | Microorganisms and methods for making 4-hydroxybutyrate, 1, 4-butanediol, and related compounds |
| ES2672883T3 (en) * | 2012-07-11 | 2018-06-18 | Institut National Des Sciences Appliquees | Modified microorganism for the production of 1,3-propanediol |
| EP2900811B1 (en) | 2012-09-26 | 2018-12-19 | Butamax Advanced Biofuels LLC | Polypeptides with ketol-acid reductoisomerase activity |
| CN104919039A (en) * | 2012-10-18 | 2015-09-16 | 阿尔吉诺生物技术有限责任公司 | Production of 1, 3-propanediol in cyanobacteria |
| WO2014062993A1 (en) * | 2012-10-18 | 2014-04-24 | Algenol Biofuels Inc. | Production of 1,2-propanediol in cyanobacteria |
| WO2014066670A1 (en) | 2012-10-24 | 2014-05-01 | Calysta Energy, Inc. | Engineering of multi-carbon substrate utilization pathways in methanotrophic bacteria |
| US20140178954A1 (en) | 2012-12-20 | 2014-06-26 | E I Du Pont De Nemours And Company | Expression of xylose isomerase activity in yeast |
| US8669076B1 (en) | 2013-03-11 | 2014-03-11 | E I Du Pont De Nemours And Company | Cow rumen xylose isomerases active in yeast cells |
| US9187743B2 (en) | 2013-03-11 | 2015-11-17 | E I Du Pont De Nemours And Company | Bacterial xylose isomerases active in yeast cells |
| US20140273105A1 (en) | 2013-03-12 | 2014-09-18 | E I Du Pont De Nemours And Company | Gradient pretreatment of lignocellulosic biomass |
| WO2014160262A1 (en) | 2013-03-14 | 2014-10-02 | Abengoa Bioenergy New Technologies, Llc | Methods for converting cellulosic waste to bioproducts |
| JP2016534131A (en) | 2013-09-03 | 2016-11-04 | ミリアント・コーポレイションＭｙｒｉａｎｔ Ｃｏｒｐｏｒａｔｉｏｎ | Process for producing acrylic acid, acrylonitrile and 1,4-butanediol from 1,3-propanediol |
| US20150147786A1 (en) | 2013-11-24 | 2015-05-28 | E I Du Pont De Nemours And Company | High force and high stress destructuring for starch biomass processing |
| KR101577503B1 (en) | 2013-12-16 | 2015-12-14 | 지에스칼텍스 주식회사 | Recombinant microorganism having enhanced 1,3-propanediol producing ability and method for producing 1,3-propanediol using the same |
| CN103789248B (en) * | 2014-02-14 | 2016-03-02 | 江苏大学 | The method of 1,3-PD is produced in a kind of 1,3-PD genetic engineering bacterium and conversion |
| WO2016007350A1 (en) | 2014-07-09 | 2016-01-14 | Danisco Us Inc. | Preconditioning of lignocellulosic biomass |
| CN104726505A (en) * | 2015-03-31 | 2015-06-24 | 上海交通大学 | Method for producing three-carbon compounds by using gene engineering cyanobacteria |
| CN104774879B (en) * | 2015-04-29 | 2017-10-20 | 大连理工大学 | A kind of method of the propane diols of mixed fungus fermentation glycerol production 1,3 |
| EP3330375A1 (en) | 2015-07-29 | 2018-06-06 | Abengoa Bioenergía Nuevas Tecnologías, S. A. | Expression of recombinant beta-xylosidase enzymes |
| US9968531B2 (en) | 2015-08-05 | 2018-05-15 | Dupont Tate & Lyle Bio Products Company, Llc | Deodorants containing 1,3-propanediol |
| BR112018070645A2 (en) | 2016-04-08 | 2019-02-05 | Du Pont | recombinant yeast cells, methods for producing a yeast cell and method for producing a target compound |
| CN106906248A (en) * | 2017-03-28 | 2017-06-30 | 清华大学 | A kind of method of the propane diols of utilization recombinant microorganism fermenting and producing 1,3 |
| CN107119003B (en) * | 2017-04-28 | 2020-04-24 | 中国科学院青岛生物能源与过程研究所 | Recombinant bacterium for synthesizing 3-hydroxypropionic acid by utilizing glucan and construction method and application thereof |
| KR101994772B1 (en) * | 2017-11-09 | 2019-07-01 | 지에스칼텍스 주식회사 | New klebsiella pneumoniae strain for production of 1,3-propanediol from glucose and method for proding 1,3-propanediol using the same |
| CN108085288B (en) * | 2017-12-22 | 2021-03-09 | 广东清大智兴生物技术有限公司 | Method for producing 1, 3-propylene glycol by utilizing recombinant microorganism fermentation |
| KR102053330B1 (en) | 2018-04-23 | 2019-12-06 | 한국과학기술연구원 | Composition for microorganism fermentation comprising glycerol and method for producing butyric acid using the same |
| KR102201720B1 (en) | 2018-05-24 | 2021-01-12 | 한화솔루션 주식회사 | Recombinant Corynebacterium Producing 1,3-PDO and Inhibited 3-HP Production and Method for Preparing 1,3-PDO Using Thereof |
| CN109321610B (en) * | 2018-11-01 | 2022-11-04 | 上海交通大学 | Efficient preparation of 2-amino-1, 3-propanediol by microbial fermentation method |
| CN110205277A (en) * | 2019-05-14 | 2019-09-06 | 河北科技师范学院 | The application of Salmonella enteritidis glpK gene delection |
| CN116064352A (en) * | 2020-03-12 | 2023-05-05 | 中国科学院上海高等研究院 | Construction method and application of Klebsiella engineering bacteria for high yield of 1, 3-propanediol |
| CN114107145A (en) * | 2021-11-04 | 2022-03-01 | 清华大学 | Recombinant microorganism and application thereof in production of 1, 3-propylene glycol |
Family Cites Families (5)
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|---|---|---|---|---|
| DE3734764A1 (en) | 1987-10-14 | 1989-05-03 | Huels Chemische Werke Ag | Process for the preparation of 1,3-propanediol |
| EP0373230B1 (en) | 1988-12-12 | 1993-02-17 | Unilever N.V. | Process for the microbiological preparation of 1,3-propane-diol from glycerol |
| US5686276A (en) | 1995-05-12 | 1997-11-11 | E. I. Du Pont De Nemours And Company | Bioconversion of a fermentable carbon source to 1,3-propanediol by a single microorganism |
| KR100509310B1 (en) | 1996-11-13 | 2005-08-22 | 이 아이 듀폰 디 네모아 앤드 캄파니 | Method for the Production of 1,3-Propanediol by recombinant Organisms |
| DE69831813T2 (en) | 1997-12-02 | 2006-07-20 | E.I. Dupont De Nemours And Co., Wilmington | METHOD OF GENERATING GLYCERINE BY RECOMOMBINANT ORGANISMS |
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