CA2384838A1 - Methods and compositions for linear isothermal amplification of polynucleotide sequences - Google Patents

Methods and compositions for linear isothermal amplification of polynucleotide sequences Download PDF

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Publication number
CA2384838A1
CA2384838A1 CA002384838A CA2384838A CA2384838A1 CA 2384838 A1 CA2384838 A1 CA 2384838A1 CA 002384838 A CA002384838 A CA 002384838A CA 2384838 A CA2384838 A CA 2384838A CA 2384838 A1 CA2384838 A1 CA 2384838A1
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CA
Canada
Prior art keywords
methods
primer
compositions
polynucleotide sequences
isothermal amplification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
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CA002384838A
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French (fr)
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CA2384838C (en
Inventor
Nurith Kurn
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Nugen Technologies Inc
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Nugen Technologies, Inc.
Nurith Kurn
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Publication of CA2384838A1 publication Critical patent/CA2384838A1/en
Application granted granted Critical
Publication of CA2384838C publication Critical patent/CA2384838C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]

Abstract

The present invention provides novel isothermal, single primer linear nucleic acid amplification methods. Methods for amplifying complementary DNA using a composite primer, primer extension, strand displacement, and optionally a termination sequence, are provided. Methods for amplifying sense RNA using a composite primer, primer extension. strand displacement, optionally template switching, a propromoter oligonucleotide and transcription are also provided. The invention further provides compositions and kits for practising said methods, as well as methods which use the amplification products.
CA002384838A 1999-09-13 2000-09-13 Methods and compositions for linear isothermal amplification of polynucleotide sequences Expired - Fee Related CA2384838C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US15360499P 1999-09-13 1999-09-13
US60/153,604 1999-09-13
US17578000P 2000-01-12 2000-01-12
US60/175,780 2000-01-12
PCT/US2000/025104 WO2001020035A2 (en) 1999-09-13 2000-09-13 Methods and compositions for linear isothermal amplification of polynucleotide sequences

Publications (2)

Publication Number Publication Date
CA2384838A1 true CA2384838A1 (en) 2001-03-22
CA2384838C CA2384838C (en) 2006-07-18

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CA002384838A Expired - Fee Related CA2384838C (en) 1999-09-13 2000-09-13 Methods and compositions for linear isothermal amplification of polynucleotide sequences

Country Status (19)

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US (2) US6251639B1 (en)
EP (2) EP1431303B1 (en)
JP (2) JP3929775B2 (en)
KR (1) KR100527265B1 (en)
CN (2) CN102586228A (en)
AT (1) ATE262595T1 (en)
AU (1) AU783873B2 (en)
BR (1) BR0014182A (en)
CA (1) CA2384838C (en)
DE (1) DE60009323T2 (en)
DK (2) DK1218542T3 (en)
ES (2) ES2447419T3 (en)
HK (1) HK1046021B (en)
IL (1) IL148091A0 (en)
MX (1) MXPA02002656A (en)
NO (1) NO20021223L (en)
NZ (1) NZ517121A (en)
PT (1) PT1218542E (en)
WO (1) WO2001020035A2 (en)

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