CA2388811A1 - Techniques for growth and differentiation of human pluripotent stem cells - Google Patents

Techniques for growth and differentiation of human pluripotent stem cells Download PDF

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Publication number
CA2388811A1
CA2388811A1 CA002388811A CA2388811A CA2388811A1 CA 2388811 A1 CA2388811 A1 CA 2388811A1 CA 002388811 A CA002388811 A CA 002388811A CA 2388811 A CA2388811 A CA 2388811A CA 2388811 A1 CA2388811 A1 CA 2388811A1
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cells
pps
pluripotent stem
human pluripotent
differentiation
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CA002388811A
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CA2388811C (en
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Melissa K. Carpenter
Walter D. Funk
Joseph D. Gold
Margaret S. Inokuma
Chunhui Xu
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Asterias Biotherapeutics Inc
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    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
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Abstract

This disclosure provides an improved system for culturing human pluripotent stem (pPS) cells in the absence of feeder cells. The role of the feeder cells can be replaced by supporting the culture on an extracellular matrix, and culturing the cells in a conditioned medium.
Permanent cell lines are provided that can produce conditioned medium on a commercial scale. Methods have also been discovered to genetically alter pPS cells by introducing the cells with a viral vector or DNA/lipid complex. The system described in this disclosure allows for bulk proliferation of pPS cells for use in studying the biology of pPS cell differentiation, and the production of important products for use in human therapy.
CA002388811A 2000-01-11 2001-01-10 Techniques for growth and differentiation of human pluripotent stem cells Expired - Lifetime CA2388811C (en)

Applications Claiming Priority (13)

Application Number Priority Date Filing Date Title
US17558100P 2000-01-11 2000-01-11
US60/175,581 2000-01-11
US21374000P 2000-06-22 2000-06-22
US21373900P 2000-06-22 2000-06-22
US60/213,739 2000-06-22
US60/213,740 2000-06-22
US21638700P 2000-07-07 2000-07-07
US60/216,387 2000-07-07
US22006400P 2000-07-21 2000-07-21
US60/220,064 2000-07-21
US09/688,031 US6667176B1 (en) 2000-01-11 2000-10-10 cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
US09/688,031 2000-10-10
PCT/US2001/001030 WO2001051616A2 (en) 2000-01-11 2001-01-10 Techniques for growth and differentiation of human pluripotent stem cells

Publications (2)

Publication Number Publication Date
CA2388811A1 true CA2388811A1 (en) 2001-07-19
CA2388811C CA2388811C (en) 2009-10-06

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CA002388811A Expired - Lifetime CA2388811C (en) 2000-01-11 2001-01-10 Techniques for growth and differentiation of human pluripotent stem cells

Country Status (8)

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US (13) US6667176B1 (en)
EP (2) EP2270504A3 (en)
JP (2) JP2003530828A (en)
CN (2) CN101792732A (en)
AU (2) AU2001226395A1 (en)
CA (1) CA2388811C (en)
IL (2) IL141742A (en)
WO (1) WO2001051616A2 (en)

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