CA2391302A1 - Method and apparatus for reducing peak broadening associated with the establishment of an electric field - Google Patents

Method and apparatus for reducing peak broadening associated with the establishment of an electric field Download PDF

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Publication number
CA2391302A1
CA2391302A1 CA002391302A CA2391302A CA2391302A1 CA 2391302 A1 CA2391302 A1 CA 2391302A1 CA 002391302 A CA002391302 A CA 002391302A CA 2391302 A CA2391302 A CA 2391302A CA 2391302 A1 CA2391302 A1 CA 2391302A1
Authority
CA
Canada
Prior art keywords
electric field
reducing peak
peak broadening
methods
establishment
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002391302A
Other languages
French (fr)
Other versions
CA2391302C (en
Inventor
Ben F. Johnson
Eric S. Nordman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems LLC
Original Assignee
Pe Corporation (Ny)
Ben F. Johnson
Eric S. Nordman
The Perkin-Elmer Corporation
Applera Corporation
Applied Biosystems Inc.
Applied Biosystems, Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pe Corporation (Ny), Ben F. Johnson, Eric S. Nordman, The Perkin-Elmer Corporation, Applera Corporation, Applied Biosystems Inc., Applied Biosystems, Llc filed Critical Pe Corporation (Ny)
Priority to CA002506142A priority Critical patent/CA2506142C/en
Publication of CA2391302A1 publication Critical patent/CA2391302A1/en
Application granted granted Critical
Publication of CA2391302C publication Critical patent/CA2391302C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44708—Cooling

Abstract

Methods for reducing peak broadening associated with the establishment of a run field in a capillary electrophoresis process, and apparatus useful for carrying out such methods, are described.
Such methods include defining a maximum ramp rate to be used during an initial electric field ramp, defining a minimum period over which the run field is established, and/or maintaining a temperature of a separation medium to within certain ranges during the initial electric field ramp. In addition, methods for determining a ramp rate effective for reducing peak broadening are disclosed.
CA002391302A 1999-07-26 2000-07-26 Method and apparatus for reducing peak broadening associated with the establishment of an electric field Expired - Fee Related CA2391302C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA002506142A CA2506142C (en) 1999-07-26 2000-07-26 Method and apparatus for reducing peak broadening associated with the establishment of an electric field

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/361,485 US6372106B1 (en) 1999-07-26 1999-07-26 Capillary electrophoresis method and apparatus for reducing peak broadening associated with the establishment of an electric field
US09/361,485 1999-07-26
PCT/US2000/020349 WO2001007904A1 (en) 1999-07-26 2000-07-26 Method and apparatus for reducing peak broadening associated with the establishment of an electric field

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA002506142A Division CA2506142C (en) 1999-07-26 2000-07-26 Method and apparatus for reducing peak broadening associated with the establishment of an electric field

Publications (2)

Publication Number Publication Date
CA2391302A1 true CA2391302A1 (en) 2001-02-01
CA2391302C CA2391302C (en) 2005-10-18

Family

ID=23422250

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002391302A Expired - Fee Related CA2391302C (en) 1999-07-26 2000-07-26 Method and apparatus for reducing peak broadening associated with the establishment of an electric field

Country Status (8)

Country Link
US (2) US6372106B1 (en)
EP (2) EP1455183B1 (en)
JP (3) JP2003505693A (en)
AT (1) ATE289683T1 (en)
AU (1) AU771703B2 (en)
CA (1) CA2391302C (en)
DE (1) DE60018283T2 (en)
WO (1) WO2001007904A1 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6372106B1 (en) * 1999-07-26 2002-04-16 Applera Corporation Capillary electrophoresis method and apparatus for reducing peak broadening associated with the establishment of an electric field
US20030143554A1 (en) * 2001-03-31 2003-07-31 Berres Mark E. Method of genotyping by determination of allele copy number
US20050133371A1 (en) * 2003-09-30 2005-06-23 West Virginia University Research Corporation Apparatus and method for Edman degradation on a microfluidic device utilizing an electroosmotic flow pump
US20050115837A1 (en) * 2003-12-01 2005-06-02 Dean Burgi Analyte identification in transformed electropherograms
US7879410B2 (en) * 2004-06-09 2011-02-01 Imra America, Inc. Method of fabricating an electrochemical device using ultrafast pulsed laser deposition
KR101269741B1 (en) * 2006-07-04 2013-05-30 쓰리엠 이노베이티브 프로퍼티즈 캄파니 Electromagnetic wave shielding gasket having elasticity and adhesiveness
WO2008009310A1 (en) * 2006-07-17 2008-01-24 Agilent Technologies, Inc. Equlibrating a temperature profile in a column
WO2008121761A1 (en) 2007-03-30 2008-10-09 Fujifilm Corporation Method of controlling temperature
WO2009098485A1 (en) * 2008-02-07 2009-08-13 Forensic Sciences Service Ltd Improvements in and relating to analysis
US20100267092A1 (en) * 2009-02-09 2010-10-21 Frederic Zenhausern Components

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Publication number Priority date Publication date Assignee Title
EP0304295A1 (en) * 1987-08-19 1989-02-22 The Board Of Trustees Of The Leland Stanford Junior University Electrokinetic analysis method and apparatus employing heat removal
JP3023793B2 (en) 1988-02-16 2000-03-21 アプライド バイオシステムズ インコーポレイテッド Capillary electrophoresis method and apparatus
EP0382426A3 (en) 1989-02-06 1992-03-25 Applied Biosystems, Inc. Micro-preparative electrophoresis apparatus
US5221448A (en) * 1990-01-25 1993-06-22 Spectra-Physics Analytical, Inc. Buffer gradient and temperature gradient capillary electrophoresis
US5164055A (en) * 1990-01-29 1992-11-17 Applied Biosystems, Inc. High-viscosity polymer matrix and methods
JPH03239959A (en) * 1990-02-16 1991-10-25 Bio Rad Lab Inc Electrophoretic separation method
JP2722752B2 (en) * 1990-02-21 1998-03-09 株式会社島津製作所 DNA detection method
JP3075602B2 (en) * 1991-09-12 2000-08-14 株式会社日立製作所 Electrophoresis device
DE9204003U1 (en) * 1992-03-24 1992-07-30 Heiss, Josef, 8172 Lenggries, De
US5296116A (en) * 1992-06-17 1994-03-22 Beckman Instruments, Inc. Capillary electrophoresis using time-varying field strength
US5409586A (en) 1992-08-26 1995-04-25 Hitachi, Ltd. Method for analyzing nucleic acid or protein and apparatus therefor
US5885432A (en) 1992-11-05 1999-03-23 Soane Biosciences Un-crosslinked polymeric media for electrophoresis
US5385654A (en) 1993-07-07 1995-01-31 Thermo Separation Products Inc. Controlled temperature anion separation by capillary electrophoresis
AU1254295A (en) 1993-12-17 1995-07-03 Perkin-Elmer Corporation, The Uncharged polymers for separation of biomolecules by capillary electrophoresis
US5512158A (en) 1995-02-28 1996-04-30 Hewlett-Packard Company Capillary electrophoresis method and apparatus for electric field uniformity and minimal dispersion of sample fractions
US5534123A (en) 1995-07-10 1996-07-09 Molecular Dynamics Denaturing separation matrix having hydroxyethyl cellulose for nucleic acid electrophoresis
JPH09178703A (en) * 1995-12-26 1997-07-11 Shimadzu Corp Base arrangement determining device
AU1375097A (en) 1995-12-28 1997-07-28 Novartis Ag Capillary electrophoretic separation method using a fillable polymer solution as separating agent
US5658446A (en) 1996-01-22 1997-08-19 Hewlett-Packard Company Preparative capillary electrophoresis with wide-bore capillary
JP3418292B2 (en) * 1996-04-24 2003-06-16 株式会社日立製作所 Gene analyzer
US5989399A (en) 1996-09-04 1999-11-23 The Research Foundation Of State University Of New York Effective surface treatment for a new separation medium in electrophoresis
US6372106B1 (en) * 1999-07-26 2002-04-16 Applera Corporation Capillary electrophoresis method and apparatus for reducing peak broadening associated with the establishment of an electric field

Also Published As

Publication number Publication date
CA2391302C (en) 2005-10-18
JP2011141297A (en) 2011-07-21
DE60018283D1 (en) 2005-03-31
JP2003505693A (en) 2003-02-12
JP2011174951A (en) 2011-09-08
US7169276B2 (en) 2007-01-30
ATE289683T1 (en) 2005-03-15
EP1196769A1 (en) 2002-04-17
AU771703B2 (en) 2004-04-01
AU6378800A (en) 2001-02-13
EP1455183A3 (en) 2004-12-29
EP1455183A2 (en) 2004-09-08
EP1455183B1 (en) 2020-08-26
US20020100689A1 (en) 2002-08-01
EP1196769B1 (en) 2005-02-23
US6372106B1 (en) 2002-04-16
WO2001007904A1 (en) 2001-02-01
DE60018283T2 (en) 2006-05-04

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