CA2395637A1 - Methods and compositions for organ decellularization - Google Patents

Methods and compositions for organ decellularization Download PDF

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Publication number
CA2395637A1
CA2395637A1 CA002395637A CA2395637A CA2395637A1 CA 2395637 A1 CA2395637 A1 CA 2395637A1 CA 002395637 A CA002395637 A CA 002395637A CA 2395637 A CA2395637 A CA 2395637A CA 2395637 A1 CA2395637 A1 CA 2395637A1
Authority
CA
Canada
Prior art keywords
organ
methods
decellularized
isolated
compositions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002395637A
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French (fr)
Other versions
CA2395637C (en
Inventor
Anthony Atala
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Childrens Medical Center Corp
Original Assignee
Children's Medical Center Corporation
Anthony Atala
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23884532&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2395637(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Children's Medical Center Corporation, Anthony Atala filed Critical Children's Medical Center Corporation
Publication of CA2395637A1 publication Critical patent/CA2395637A1/en
Application granted granted Critical
Publication of CA2395637C publication Critical patent/CA2395637C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
    • A61L27/3691—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by physical conditions of the treatment, e.g. applying a compressive force to the composition, pressure cycles, ultrasonic/sonication or microwave treatment, lyophilisation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
    • A61L27/3687—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by the use of chemical agents in the treatment, e.g. specific enzymes, detergents, capping agents, crosslinkers, anticalcification agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/26—Materials or treatment for tissue regeneration for kidney reconstruction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/40—Preparation and treatment of biological tissue for implantation, e.g. decellularisation, cross-linking

Abstract

The invention is directed to methods for producing a decellularized organ or part of an organ. A decellularized organ is produced using an isolated organ mechanically agitated to remove cellular membranes surrounding the isolated organ without destroying the interstitial structure of the organ. After the cellular membrane is removed, the isolated organ is exposed to a solubilizing fluid that extracts cellular material without dissolving the interstitial structure of the organ. A washing fluid is used to remove the solubilized components,leaving behind a decellularized organ.
CA002395637A 1999-12-29 2000-12-14 Methods and compositions for organ decellularization Expired - Lifetime CA2395637C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/474,678 1999-12-29
US09/474,678 US6376244B1 (en) 1999-12-29 1999-12-29 Methods and compositions for organ decellularization
PCT/US2000/033782 WO2001049210A1 (en) 1999-12-29 2000-12-14 Methods and compositions for organ decellularization

Publications (2)

Publication Number Publication Date
CA2395637A1 true CA2395637A1 (en) 2001-07-12
CA2395637C CA2395637C (en) 2005-05-24

Family

ID=23884532

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002395637A Expired - Lifetime CA2395637C (en) 1999-12-29 2000-12-14 Methods and compositions for organ decellularization

Country Status (9)

Country Link
US (3) US6376244B1 (en)
EP (1) EP1244396B2 (en)
JP (1) JP4897176B2 (en)
AT (1) ATE308939T1 (en)
AU (1) AU763730B2 (en)
CA (1) CA2395637C (en)
DE (1) DE60023941D1 (en)
ES (1) ES2250220T5 (en)
WO (1) WO2001049210A1 (en)

Families Citing this family (148)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SK22499A3 (en) 1996-08-23 1999-10-08 Cook Biotech Inc Graft prosthesis, materials and methods
DE69817863T2 (en) * 1997-10-31 2004-07-15 Children's Medical Center Corp., Boston BLADDER RECONSTRUCTION
US6293970B1 (en) 1998-06-30 2001-09-25 Lifenet Plasticized bone and soft tissue grafts and methods of making and using same
US6734018B2 (en) * 1999-06-07 2004-05-11 Lifenet Process for decellularizing soft-tissue engineered medical implants, and decellularized soft-tissue medical implants produced
US8563232B2 (en) * 2000-09-12 2013-10-22 Lifenet Health Process for devitalizing soft-tissue engineered medical implants, and devitalized soft-tissue medical implants produced
US20100030340A1 (en) * 1998-06-30 2010-02-04 Wolfinbarger Jr Lloyd Plasticized Grafts and Methods of Making and Using Same
US7063726B2 (en) * 1998-06-30 2006-06-20 Lifenet Plasticized bone grafts and methods of making and using same
US6743574B1 (en) 2000-09-12 2004-06-01 Lifenet Process for devitalizing soft-tissue engineered medical implants, and devitalized soft-tissue medical implants produced
US20030217415A1 (en) * 1998-06-30 2003-11-27 Katrina Crouch Plasticized bone grafts and methods of making and using same
US6214054B1 (en) * 1998-09-21 2001-04-10 Edwards Lifesciences Corporation Method for fixation of biological tissues having mitigated propensity for post-implantation calcification and thrombosis and bioprosthetic devices prepared thereby
US20040167634A1 (en) * 1999-05-26 2004-08-26 Anthony Atala Prosthetic kidney and its use for treating kidney disease
US6576265B1 (en) 1999-12-22 2003-06-10 Acell, Inc. Tissue regenerative composition, method of making, and method of use thereof
US20040043006A1 (en) * 2002-08-27 2004-03-04 Badylak Stephen F. Tissue regenerative composition
US20050249816A1 (en) * 1999-12-29 2005-11-10 Wake Forest University Health Services Tissue engineered liver constructs
US6376244B1 (en) * 1999-12-29 2002-04-23 Children's Medical Center Corporation Methods and compositions for organ decellularization
WO2002014480A2 (en) * 2000-08-16 2002-02-21 Duke University Decellularized tissue engineered constructs and tissues
US20020119437A1 (en) * 2000-09-20 2002-08-29 Grooms Jamie M. Method of preparing and processing transplant tissue
IL139708A0 (en) * 2000-11-15 2002-02-10 Amiel Gilad Process of decellularizing biological matrices and acellular biological matrices useful in tissue engineering
US8039261B2 (en) * 2000-11-17 2011-10-18 Vascular Biogenics Ltd. Promoters exhibiting endothelial cell specificity and methods of using same for regulation of angiogenesis
US8071740B2 (en) * 2000-11-17 2011-12-06 Vascular Biogenics Ltd. Promoters exhibiting endothelial cell specificity and methods of using same for regulation of angiogenesis
US20100282634A1 (en) * 2000-11-17 2010-11-11 Dror Harats Promoters Exhibiting Endothelial Cell Specificity and Methods of Using Same for Regulation of Angiogenesis
AU2003222427B8 (en) * 2000-11-17 2010-04-29 Vascular Biogenics Ltd. Promoters exhibiting endothelial cell specificity and methods of using same
US20070286845A1 (en) * 2000-11-17 2007-12-13 Vascular Biogenics Ltd. Promoters exhibiting endothelial cell specificity and methods of using same for regulation of angiogenesis
US6838452B2 (en) 2000-11-24 2005-01-04 Vascular Biogenics Ltd. Methods employing and compositions containing defined oxidized phospholipids for prevention and treatment of atherosclerosis
DE10064948C1 (en) * 2000-12-20 2002-07-11 Auto Tissue Gmbh Process for decellularizing foreign material for the production of bioprostheses and device for carrying out the process
KR100973615B1 (en) * 2001-02-14 2010-08-02 안트로제네시스 코포레이션 Post-partum mammalian placenta, its use and placental stem cells therefrom
EP1404388B1 (en) * 2001-06-28 2013-10-23 Cook Biotech, Inc. Graft prosthesis devices containing renal capsule collagen
DE10138564B4 (en) * 2001-08-06 2007-07-12 Lamm, Peter Wilhelm, Dr. Method of devitalizing natural organs and / or providing extracellular matrices for tissue engineering
JP4173446B2 (en) 2001-10-19 2008-10-29 ヴァスキュラー バイオジェニックス リミテッド Polynucleotide constructs and pharmaceutical compositions and methods for targeted down-regulation of angiogenesis and anti-cancer therapy
EP1317934A1 (en) * 2001-11-08 2003-06-11 Artiss GmbH Process for the preparation of an acellularized tissue matrix and the resulting tissue matrix
WO2003043674A1 (en) * 2001-11-16 2003-05-30 Children's Medical Center Corporation ¨ Augmentation of organ function
US6878168B2 (en) 2002-01-03 2005-04-12 Edwards Lifesciences Corporation Treatment of bioprosthetic tissues to mitigate post implantation calcification
US8308797B2 (en) 2002-01-04 2012-11-13 Colibri Heart Valve, LLC Percutaneously implantable replacement heart valve device and method of making same
US20030187515A1 (en) * 2002-03-26 2003-10-02 Hariri Robert J. Collagen biofabric and methods of preparing and using the collagen biofabric
US20030215935A1 (en) * 2002-05-14 2003-11-20 Coon David James Apparatus and method for isolating living cells from an encapsulated organ tissue sample
EP1517715A2 (en) * 2002-06-28 2005-03-30 Cardio, Inc. Decellularized tissue
US7402319B2 (en) * 2002-09-27 2008-07-22 Board Of Regents, The University Of Texas System Cell-free tissue replacement for tissue engineering
US8940292B2 (en) 2003-01-28 2015-01-27 Wake Forest University Health Sciences Enhancement of angiogenesis to grafts using cells engineered to produce growth factors
US20040176855A1 (en) * 2003-03-07 2004-09-09 Acell, Inc. Decellularized liver for repair of tissue and treatment of organ deficiency
US20040175366A1 (en) * 2003-03-07 2004-09-09 Acell, Inc. Scaffold for cell growth and differentiation
US7067123B2 (en) 2003-04-29 2006-06-27 Musculoskeletal Transplant Foundation Glue for cartilage repair
US7901457B2 (en) 2003-05-16 2011-03-08 Musculoskeletal Transplant Foundation Cartilage allograft plug
US7488348B2 (en) * 2003-05-16 2009-02-10 Musculoskeletal Transplant Foundation Cartilage allograft plug
US20050025838A1 (en) * 2003-06-25 2005-02-03 Badylak Stephen F. Conditioned compositions for tissue restoration
US20050054099A1 (en) * 2003-09-04 2005-03-10 Cedars Sinai Medical Center Matrix derived from whole organ
EP1677703A4 (en) * 2003-10-02 2009-09-02 Depuy Spine Inc Chemical treatment for removing cellular and nuclear material from naturally occurring extracellular matrix-based biomaterials
US7775965B2 (en) * 2004-03-09 2010-08-17 The Board Of Regents Of The University Of Oklahoma Decellularized grafts from umbilical cord vessels and process for preparing and using same
US7837740B2 (en) 2007-01-24 2010-11-23 Musculoskeletal Transplant Foundation Two piece cancellous construct for cartilage repair
US7759120B2 (en) * 2005-03-02 2010-07-20 Kps Bay Medical, Inc. Seeding implantable medical devices with cells
US20060199265A1 (en) * 2005-03-02 2006-09-07 Wolf Michael F Seeding implantable medical devices with cells
US9493735B2 (en) 2005-04-15 2016-11-15 Wake Forest University Health Sciences Bioreactor system and method of enhancing functionality of muscle cultured in vitro
US9788821B2 (en) * 2005-04-29 2017-10-17 Cook Biotech Incorporated Physically modified extracellular matrix materials and uses thereof
DE102005023599A1 (en) * 2005-05-18 2006-11-23 Corlife Gbr (Vertretungsberechtigte Gesellschafter: Prof. Dr. Alex Haverich Bioartificial heart tissue graft and process for its preparation
CN101252957A (en) * 2005-06-30 2008-08-27 人类起源公司 Repair of tympanic membrane using placenta derived collagen biofabric
US7815926B2 (en) 2005-07-11 2010-10-19 Musculoskeletal Transplant Foundation Implant for articular cartilage repair
PL1928519T3 (en) * 2005-08-26 2012-08-31 Univ Minnesota Decellularization and recellularization of organs and tissues
AU2015224503A1 (en) * 2005-08-26 2015-10-01 Regents Of The University Of Minnesota Decellularization and recellularization of organs and tissues
US20100093066A1 (en) * 2005-08-26 2010-04-15 Regents Of The University Of Minnesota Decellularization and recellularization apparatuses and systems containing the same
AU2013224686B2 (en) * 2005-08-26 2015-06-11 Miromatrix Medical Inc. Decellularization and recellularization of organs and tissues
AU2006292224B2 (en) 2005-09-19 2013-08-01 Histogenics Corporation Cell-support matrix and a method for preparation thereof
US20070143143A1 (en) * 2005-12-16 2007-06-21 Siemens Medical Solutions Health Services Corporation Patient Discharge Data Processing System
EP2031968B1 (en) * 2006-04-21 2017-11-22 Wake Forest University Health Sciences Structurally modified acellular tissue engineering scaffolds and methods of production
CN101501185A (en) * 2006-06-09 2009-08-05 人类起源公司 Placental niche and use thereof to culture stem cells
WO2008021391A1 (en) * 2006-08-15 2008-02-21 Anthrogenesis Corporation Umbilical cord biomaterial for medical use
US20080131522A1 (en) * 2006-10-03 2008-06-05 Qing Liu Use of placental biomaterial for ocular surgery
WO2008060377A2 (en) 2006-10-04 2008-05-22 Anthrogenesis Corporation Placental or umbilical cord tissue compositions
KR20210018526A (en) 2006-10-06 2021-02-17 안트로제네시스 코포레이션 Native(telopeptide) Placental Collagen Compositions
CA2666485C (en) 2006-10-27 2015-10-06 Edwards Lifesciences Corporation Biological tissue for surgical implantation
ES2372116T3 (en) * 2006-11-16 2012-01-16 The University Of Leeds PREPARATION OF FABRIC FOR MENISCAL IMPLEMENTATION.
US8435551B2 (en) 2007-03-06 2013-05-07 Musculoskeletal Transplant Foundation Cancellous construct with support ring for repair of osteochondral defects
EP2138181B1 (en) * 2007-03-09 2016-05-04 National University Corporation Tokyo Medical and Dental University Method of preparing decellularized soft tissue
BRPI0810949A2 (en) 2007-05-29 2015-10-27 Christopher B Reid "Method of preparing multipotent, self-renewing, differentiating or disease resistant cells, cell and vector for use of the method"
US9580688B2 (en) * 2007-06-08 2017-02-28 Wake Forest University Health Sciences Kidney structures and methods of forming the same
JP2010528658A (en) 2007-06-08 2010-08-26 ウェイク・フォレスト・ユニヴァーシティ・ヘルス・サイエンシズ Selective cell therapy for the treatment of renal failure
US9101691B2 (en) * 2007-06-11 2015-08-11 Edwards Lifesciences Corporation Methods for pre-stressing and capping bioprosthetic tissue
US9744043B2 (en) 2007-07-16 2017-08-29 Lifenet Health Crafting of cartilage
JP2009050297A (en) * 2007-08-23 2009-03-12 Tokyo Medical & Dental Univ Decellularization process liquid, preparing procedure of decellularization process tissue, implant, and cultivating member
JP5218955B2 (en) * 2007-10-15 2013-06-26 独立行政法人物質・材料研究機構 Porous scaffold for regeneration and method for producing the same
US8357387B2 (en) 2007-12-21 2013-01-22 Edwards Lifesciences Corporation Capping bioprosthetic tissue to reduce calcification
US11051733B2 (en) * 2008-01-18 2021-07-06 Wake Forest University Health Sciences Isolating and purifying cells for therapy
CA2717725A1 (en) 2008-03-05 2009-09-11 Musculoskeletal Transplant Foundation Cancellous constructs, cartilage particles and combinations of cancellous constructs and cartilage particles
CA2734560C (en) * 2008-06-20 2018-10-23 Cook Biotech Incorporated Composite extracellular matrix materials and medical products formed therefrom
CA2734446C (en) 2008-08-22 2017-06-20 Anthrogenesis Corporation Methods and compositions for treatment of bone defects with placental cell populations
KR20180034707A (en) 2008-11-04 2018-04-04 인리젠 Cell-scaffold constructs
KR20220112862A (en) 2008-11-12 2022-08-11 인리젠 Isolated renal cells and uses thereof
WO2010057013A1 (en) * 2008-11-14 2010-05-20 Wake Forest University Health Sciences Selective cell therapy for the treatment of renal failure
US9150318B1 (en) 2009-01-02 2015-10-06 Lifecell Corporation Method for sterilizing an acellular tissue matrix
WO2010099310A1 (en) 2009-02-26 2010-09-02 Wake Forest University Health Sciences Endothelial scaffolds
US8906362B2 (en) 2009-03-23 2014-12-09 Wake Forest University Health Sciences Tissue engineered meniscus scaffolds and methods of use
US20110045045A1 (en) * 2009-07-07 2011-02-24 Joaquin Cortiella Production of and uses for decellularized lung tissue
US8652500B2 (en) 2009-07-22 2014-02-18 Acell, Inc. Particulate tissue graft with components of differing density and methods of making and using the same
US8298586B2 (en) 2009-07-22 2012-10-30 Acell Inc Variable density tissue graft composition
US8507263B2 (en) * 2009-08-07 2013-08-13 Maria Adelaide Asnaghi Rotating bioreactor
JP2011041716A (en) * 2009-08-21 2011-03-03 Cell Remover:Kk Method of processing living tissue
US20110236949A1 (en) * 2009-09-22 2011-09-29 Colorado State University Research Foundation Methods for Processing Biological Tissues
HUE038789T2 (en) 2009-10-07 2018-11-28 Kerecis Ehf A scaffold material for wound care and/or other tissue healing applications
JP2011135836A (en) * 2009-12-28 2011-07-14 Cell Remover:Kk Cell culture base material
EP2542668A2 (en) * 2010-03-01 2013-01-09 Colibri Heart Valve LLC Tissue for prosthetic implants and grafts, and methods associated therewith
BR112012023769B1 (en) 2010-03-23 2020-11-10 Edwards Lifesciences Corporation method for preparing bioprosthetic tissue membrane material
WO2011127113A1 (en) 2010-04-08 2011-10-13 Anthrogenesis Corporation Treatment of sarcoidosis using placental stem cells
US9352003B1 (en) 2010-05-14 2016-05-31 Musculoskeletal Transplant Foundation Tissue-derived tissuegenic implants, and methods of fabricating and using same
US10130736B1 (en) 2010-05-14 2018-11-20 Musculoskeletal Transplant Foundation Tissue-derived tissuegenic implants, and methods of fabricating and using same
US8883210B1 (en) * 2010-05-14 2014-11-11 Musculoskeletal Transplant Foundation Tissue-derived tissuegenic implants, and methods of fabricating and using same
US8906601B2 (en) 2010-06-17 2014-12-09 Edwardss Lifesciences Corporation Methods for stabilizing a bioprosthetic tissue by chemical modification of antigenic carbohydrates
CA2806544C (en) 2010-06-28 2016-08-23 Colibri Heart Valve Llc Method and apparatus for the endoluminal delivery of intravascular devices
SG188369A1 (en) 2010-09-01 2013-04-30 Univ Minnesota Methods of recellularizing a tissue or organ for improved transplantability
AU2011326767B2 (en) 2010-11-10 2016-02-11 Prokidney Injectable formulations for organ augmentation
US9351829B2 (en) 2010-11-17 2016-05-31 Edwards Lifesciences Corporation Double cross-linkage process to enhance post-implantation bioprosthetic tissue durability
CA3027755C (en) 2010-12-14 2021-05-11 Colibri Heart Valve Llc Percutaneously deliverable heart valve including folded membrane cusps with integral leaflets
AU2011352036A1 (en) 2010-12-31 2013-07-18 Anthrogenesis Corporation Enhancement of placental stem cell potency using modulatory RNA molecules
CN113559126A (en) 2011-06-01 2021-10-29 人类起源公司 Treatment of pain using placental stem cells
US20130143323A1 (en) * 2011-06-01 2013-06-06 Geoffrey S. FROST Apparatus and method for decellularizing, recellularizing or treating organs
WO2013089808A1 (en) * 2011-12-14 2013-06-20 Edmund Burke Decellularized small particle tissue
US20130288973A1 (en) * 2011-12-14 2013-10-31 Edmund Burke Decellularized small particle tissue
US9295756B2 (en) 2012-02-01 2016-03-29 Nayacure Therapeutics Ltd. Methods for inducing immune tolerance to organ transplants
AU2013234037A1 (en) 2012-03-16 2014-10-02 Novahep Ab Bioengineered allogeneic blood vessel
US9877822B2 (en) 2012-04-24 2018-01-30 Biostage, Inc. Engineered tissue scaffolds and supports therefor
US9290738B2 (en) 2012-06-13 2016-03-22 Miromatrix Medical Inc. Methods of decellularizing bone
US20140377227A1 (en) * 2012-11-07 2014-12-25 James Bennie Gandy Tissue Transplantation Involving, and Tissues Modified by Decellularization And Recellularization Of Donor Tissues For Minimized Or Obviated Rejection Reactions
US9439949B2 (en) * 2012-11-07 2016-09-13 Gray Reed & Mcgraw, P.C. Decellularization and recellularization of donor tissues for minimized or obviated rejection reactions
US10238771B2 (en) 2012-11-08 2019-03-26 Edwards Lifesciences Corporation Methods for treating bioprosthetic tissue using a nucleophile/electrophile in a catalytic system
WO2014110300A1 (en) 2013-01-09 2014-07-17 Harvard Apparatus Regenerative Technology Synthetic scaffolds
US9289452B2 (en) 2013-03-07 2016-03-22 Allosource Consistent calcium content bone allograft systems and methods
MX2015013154A (en) 2013-03-15 2016-07-13 Miromatrix Medical Inc Use of perfusion decellularized liver for islet cell recellularization.
WO2014181767A1 (en) 2013-05-07 2014-11-13 国立大学法人 東京医科歯科大学 Method for producing particulate decellularized tissue
US9615922B2 (en) 2013-09-30 2017-04-11 Edwards Lifesciences Corporation Method and apparatus for preparing a contoured biological tissue
US10959839B2 (en) 2013-10-08 2021-03-30 Edwards Lifesciences Corporation Method for directing cellular migration patterns on a biological tissue
WO2015181245A1 (en) 2014-05-27 2015-12-03 Novahep Ab Bioengineered allogeneic valve
CN104208751A (en) * 2014-08-19 2014-12-17 温州医科大学 Preparation method for novel kidney acellularized biological scaffold
US10077420B2 (en) 2014-12-02 2018-09-18 Histogenics Corporation Cell and tissue culture container
US9238090B1 (en) 2014-12-24 2016-01-19 Fettech, Llc Tissue-based compositions
EP3263140B1 (en) 2015-02-27 2021-03-31 Adeka Corporation Decellularized tissue
CN107530476A (en) 2015-03-12 2018-01-02 般财团法人化学及血清疗法研究所 Anti-adhering material and alternative biomembrane using acellular tissue
US10531957B2 (en) 2015-05-21 2020-01-14 Musculoskeletal Transplant Foundation Modified demineralized cortical bone fibers
GB201513461D0 (en) * 2015-07-30 2015-09-16 Ucl Business Plc Methods and devices for the production of decellularised tissue scaffolds
EP3640321B1 (en) 2015-10-09 2022-04-06 DEKA Products Limited Partnership Method for generating a tissue for transplant
CN109715140B (en) 2016-06-15 2022-06-24 通用医疗公司 Metabolic labeling and molecular enhancement of biological materials using bio-orthogonal reactions
US11123372B2 (en) 2016-07-29 2021-09-21 Prokidney Bioactive renal cells for the treatment of chronic kidney disease
WO2018048899A1 (en) 2016-09-06 2018-03-15 Micromatrix Medical Inc. Use of resected liver serum for whole liver engineering
US11299705B2 (en) 2016-11-07 2022-04-12 Deka Products Limited Partnership System and method for creating tissue
WO2018127554A1 (en) 2017-01-06 2018-07-12 Novahep Ab Methods of preparing bioengineered or bioprinted organ or tissue, and uses thereof
US20200222589A1 (en) 2017-05-30 2020-07-16 Adeka Corporation Method for producing decellularized material for transplantation and graft composition consisting of biocompatible material including said material
WO2019051476A1 (en) 2017-09-11 2019-03-14 Incubar, LLC Conduit vascular implant sealing device for reducing endoleak
CN107596446B (en) * 2017-09-16 2020-07-28 温州医科大学 Decellularization kit for maintaining tissue ultrastructure and nutrition microenvironment
WO2020026212A2 (en) 2018-08-03 2020-02-06 VeriGraft AB Methods of preparing personalized blood vessels
WO2020092205A1 (en) 2018-11-01 2020-05-07 Edwards Lifesciences Corporation Transcatheter pulmonic regenerative valve
EP3908339A1 (en) 2019-01-07 2021-11-17 Miromatrix Medical Inc. Method of dehydration of extracellular matrix and particles formed therefrom
WO2022030856A1 (en) 2020-08-06 2022-02-10 주식회사 강스템바이오텍 Method for producing highly functional artificial organs using aptamers

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458678A (en) 1981-10-26 1984-07-10 Massachusetts Institute Of Technology Cell-seeding procedures involving fibrous lattices
US4520821A (en) 1982-04-30 1985-06-04 The Regents Of The University Of California Growing of long-term biological tissue correction structures in vivo
US4801299A (en) 1983-06-10 1989-01-31 University Patents, Inc. Body implants of extracellular matrix and means and methods of making and using such implants
US5032508A (en) 1988-09-08 1991-07-16 Marrow-Tech, Inc. Three-dimensional cell and tissue culture system
US4963489A (en) 1987-04-14 1990-10-16 Marrow-Tech, Inc. Three-dimensional cell and tissue culture system
US5863531A (en) 1986-04-18 1999-01-26 Advanced Tissue Sciences, Inc. In vitro preparation of tubular tissue structures by stromal cell culture on a three-dimensional framework
US5266480A (en) 1986-04-18 1993-11-30 Advanced Tissue Sciences, Inc. Three-dimensional skin culture system
US5160490A (en) 1986-04-18 1992-11-03 Marrow-Tech Incorporated Three-dimensional cell and tissue culture apparatus
US5902741A (en) 1986-04-18 1999-05-11 Advanced Tissue Sciences, Inc. Three-dimensional cartilage cultures
US5567612A (en) 1986-11-20 1996-10-22 Massachusetts Institute Of Technology Genitourinary cell-matrix structure for implantation into a human and a method of making
US5759830A (en) 1986-11-20 1998-06-02 Massachusetts Institute Of Technology Three-dimensional fibrous scaffold containing attached cells for producing vascularized tissue in vivo
CA1340581C (en) 1986-11-20 1999-06-08 Joseph P. Vacanti Chimeric neomorphogenesis of organs by controlled cellular implantation using artificial matrices
US5336616A (en) * 1990-09-12 1994-08-09 Lifecell Corporation Method for processing and preserving collagen-based tissues for transplantation
US5192312A (en) 1991-03-05 1993-03-09 Colorado State University Research Foundation Treated tissue for implantation and methods of treatment and use
AU2900792A (en) 1991-10-24 1993-05-21 Children's Medical Center Corporation Neomorphogenesis of urological structures in vivo from cell culture
ATE265191T1 (en) 1994-03-14 2004-05-15 Cryolife Inc PRODUCTION PROCESS OF TISSUE FOR IMPLANTATION
US5916265A (en) 1994-03-30 1999-06-29 Hu; Jie Method of producing a biological extracellular matrix for use as a cell seeding scaffold and implant
US5695998A (en) 1995-02-10 1997-12-09 Purdue Research Foundation Submucosa as a growth substrate for islet cells
JPH11508151A (en) 1995-04-07 1999-07-21 パーデュー・リサーチ・ファウンデーション Bladder reconstruction method and tissue graft for bladder reconstruction
EP0821573A4 (en) * 1995-04-19 2000-08-09 St Jude Medical Matrix substrate for a viable body tissue-derived prosthesis and method for making the same
US5855610A (en) 1995-05-19 1999-01-05 Children's Medical Center Corporation Engineering of strong, pliable tissues
ES2175753T3 (en) * 1997-06-27 2002-11-16 Augustinus Bader BIOSYNTHETIC GRAFT AND METHOD FOR PRODUCTION.
US6376244B1 (en) * 1999-12-29 2002-04-23 Children's Medical Center Corporation Methods and compositions for organ decellularization

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