CA2395698A1 - Reconstructing organs from decellularized biomaterial scaffold - Google Patents
Reconstructing organs from decellularized biomaterial scaffold Download PDFInfo
- Publication number
- CA2395698A1 CA2395698A1 CA002395698A CA2395698A CA2395698A1 CA 2395698 A1 CA2395698 A1 CA 2395698A1 CA 002395698 A CA002395698 A CA 002395698A CA 2395698 A CA2395698 A CA 2395698A CA 2395698 A1 CA2395698 A1 CA 2395698A1
- Authority
- CA
- Canada
- Prior art keywords
- organ
- scaffold
- reconstructing
- cell population
- vascular system
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3886—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells comprising two or more cell types
- A61L27/3891—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells comprising two or more cell types as distinct cell layers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
- A61L27/3808—Endothelial cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/18—Drugs for disorders of the alimentary tract or the digestive system for pancreatic disorders, e.g. pancreatic enzymes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P13/00—Drugs for disorders of the urinary system
- A61P13/02—Drugs for disorders of the urinary system of urine or of the urinary tract, e.g. urine acidifiers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P13/00—Drugs for disorders of the urinary system
- A61P13/10—Drugs for disorders of the urinary system of the bladder
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P13/00—Drugs for disorders of the urinary system
- A61P13/12—Drugs for disorders of the urinary system of the kidneys
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0684—Cells of the urinary tract or kidneys
- C12N5/0686—Kidney cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/28—Vascular endothelial cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2503/00—Use of cells in diagnostics
- C12N2503/04—Screening or testing on artificial tissues
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/52—Fibronectin; Laminin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/54—Collagen; Gelatin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
Abstract
The invention is directed to compositions and methods for reconstructing an artificial organ. The artificial organ is reconstructed using a three-dimensional scaffold produced by decellularizing biostructures from a donor organ. The three-dimen-sional scaffold is perfused with endothelial cells that develop to produce an endothelial tissue layer with a primitive vascular system that sustains the growth and development of a second cultured cell population.
When grown in the three-dimensional scaffold and endothelial tissue layer, with the primitive vascular system, the cells of the second cell population proliferate, mature and differentiate into neomorphic organ structures that are analogous to their in vivo counterparts.
When grown in the three-dimensional scaffold and endothelial tissue layer, with the primitive vascular system, the cells of the second cell population proliferate, mature and differentiate into neomorphic organ structures that are analogous to their in vivo counterparts.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/474,525 | 1999-12-29 | ||
US09/474,525 US6479064B1 (en) | 1999-12-29 | 1999-12-29 | Culturing different cell populations on a decellularized natural biostructure for organ reconstruction |
PCT/US2000/033891 WO2001048153A1 (en) | 1999-12-29 | 2000-12-14 | Reconstructing organs from decellularized biomaterial scaffold |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2395698A1 true CA2395698A1 (en) | 2001-07-05 |
CA2395698C CA2395698C (en) | 2008-01-29 |
Family
ID=23883893
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002395698A Expired - Lifetime CA2395698C (en) | 1999-12-29 | 2000-12-14 | Reconstructing organs from decellularized biomaterial scaffold |
Country Status (8)
Country | Link |
---|---|
US (1) | US6479064B1 (en) |
EP (1) | EP1246903B1 (en) |
JP (1) | JP2003518517A (en) |
AT (1) | ATE384782T1 (en) |
AU (1) | AU770887B2 (en) |
CA (1) | CA2395698C (en) |
DE (1) | DE60037909T2 (en) |
WO (1) | WO2001048153A1 (en) |
Families Citing this family (153)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6485723B1 (en) | 1995-02-10 | 2002-11-26 | Purdue Research Foundation | Enhanced submucosal tissue graft constructs |
US8563232B2 (en) | 2000-09-12 | 2013-10-22 | Lifenet Health | Process for devitalizing soft-tissue engineered medical implants, and devitalized soft-tissue medical implants produced |
US7063726B2 (en) * | 1998-06-30 | 2006-06-20 | Lifenet | Plasticized bone grafts and methods of making and using same |
US6293970B1 (en) | 1998-06-30 | 2001-09-25 | Lifenet | Plasticized bone and soft tissue grafts and methods of making and using same |
US6734018B2 (en) * | 1999-06-07 | 2004-05-11 | Lifenet | Process for decellularizing soft-tissue engineered medical implants, and decellularized soft-tissue medical implants produced |
US6743574B1 (en) | 2000-09-12 | 2004-06-01 | Lifenet | Process for devitalizing soft-tissue engineered medical implants, and devitalized soft-tissue medical implants produced |
US7759113B2 (en) | 1999-04-30 | 2010-07-20 | The General Hospital Corporation | Fabrication of tissue lamina using microfabricated two-dimensional molds |
US6576265B1 (en) | 1999-12-22 | 2003-06-10 | Acell, Inc. | Tissue regenerative composition, method of making, and method of use thereof |
US20040043006A1 (en) * | 2002-08-27 | 2004-03-04 | Badylak Stephen F. | Tissue regenerative composition |
US7776021B2 (en) | 2000-04-28 | 2010-08-17 | The Charles Stark Draper Laboratory | Micromachined bilayer unit for filtration of small molecules |
US20100282634A1 (en) * | 2000-11-17 | 2010-11-11 | Dror Harats | Promoters Exhibiting Endothelial Cell Specificity and Methods of Using Same for Regulation of Angiogenesis |
US8039261B2 (en) * | 2000-11-17 | 2011-10-18 | Vascular Biogenics Ltd. | Promoters exhibiting endothelial cell specificity and methods of using same for regulation of angiogenesis |
AU2003222427B8 (en) * | 2000-11-17 | 2010-04-29 | Vascular Biogenics Ltd. | Promoters exhibiting endothelial cell specificity and methods of using same |
US8071740B2 (en) | 2000-11-17 | 2011-12-06 | Vascular Biogenics Ltd. | Promoters exhibiting endothelial cell specificity and methods of using same for regulation of angiogenesis |
US20070286845A1 (en) * | 2000-11-17 | 2007-12-13 | Vascular Biogenics Ltd. | Promoters exhibiting endothelial cell specificity and methods of using same for regulation of angiogenesis |
US6838452B2 (en) * | 2000-11-24 | 2005-01-04 | Vascular Biogenics Ltd. | Methods employing and compositions containing defined oxidized phospholipids for prevention and treatment of atherosclerosis |
ES2272365T5 (en) * | 2001-02-13 | 2011-04-26 | Corlife Gbr | PRIMARY VASCULARIZED BIOARTIFICIAL TISSUE MATRIX AND PRIMARY VASCULARIZED BIOARTIFICIAL TISSUE. |
US6994706B2 (en) | 2001-08-13 | 2006-02-07 | Minnesota Medical Physics, Llc | Apparatus and method for treatment of benign prostatic hyperplasia |
AU2002323526A1 (en) * | 2001-08-31 | 2003-03-18 | Pacgen Cellco Llc | Use of recipient endothelial cells for enhanced vascularization of tissue and tissue-engineered construct transplants |
EP1436313B1 (en) * | 2001-10-19 | 2010-09-22 | Vascular Biogenics Ltd. | Polynucleotide constructs, pharmaceutical compositions and methods for targeted downregulation of angiogenesis and anticancer therapy |
AU2007201136B2 (en) * | 2001-11-16 | 2011-03-03 | Children's Medical Center Corporation | Augmentation of organ function |
JP2005509495A (en) * | 2001-11-16 | 2005-04-14 | チルドレンズ メディカル センター コーポレーション | Enhancement of organ function |
FR2833610B1 (en) * | 2001-12-14 | 2007-01-26 | Natural Implant | IN VITRO CULTIVE CELL CONSTRUCTS, PREPARATION AND USES |
US20030187515A1 (en) * | 2002-03-26 | 2003-10-02 | Hariri Robert J. | Collagen biofabric and methods of preparing and using the collagen biofabric |
US9144583B2 (en) | 2002-03-29 | 2015-09-29 | Tissue Genesis, Inc. | Cell separation apparatus and methods of use |
US8202725B2 (en) * | 2004-12-23 | 2012-06-19 | Tissue Genesis Incorporated | Cell sodding method and apparatus |
CN1684589A (en) | 2002-05-02 | 2005-10-19 | 普渡研究基金会 | Vascularization enhanced graft constructs |
NZ536611A (en) * | 2002-05-02 | 2006-04-28 | Purdue Research Foundation | Vascularization enhanced intestinal submucosa tissue graft constructs prepared by seeding intestinal submucosa tissue in vitro with endothelial cells or endothelial cell precursors |
WO2003092604A2 (en) | 2002-05-02 | 2003-11-13 | Purdue Research Foundation | Vascularization enhanced graft constructs |
US7299805B2 (en) | 2002-06-07 | 2007-11-27 | Marctec, Llc | Scaffold and method for implanting cells |
CN1668734A (en) * | 2002-06-20 | 2005-09-14 | 比奥索斯控股有限公司 | Method and device for multiplying and differentiating cells in the presence of growth factors and of a biological matrix or of a supporting structure |
WO2004003178A2 (en) * | 2002-06-28 | 2004-01-08 | Cardio, Inc. | Decellularized tissue |
WO2004065616A2 (en) * | 2003-01-16 | 2004-08-05 | The General Hospital Corporation | Use of three-dimensional microfabricated tissue engineered systems for pharmacologic applications |
US8940292B2 (en) | 2003-01-28 | 2015-01-27 | Wake Forest University Health Sciences | Enhancement of angiogenesis to grafts using cells engineered to produce growth factors |
US20040175366A1 (en) * | 2003-03-07 | 2004-09-09 | Acell, Inc. | Scaffold for cell growth and differentiation |
US20040176855A1 (en) * | 2003-03-07 | 2004-09-09 | Acell, Inc. | Decellularized liver for repair of tissue and treatment of organ deficiency |
EP1479760A1 (en) * | 2003-05-19 | 2004-11-24 | ProBioGen AG | Artificial immune organ |
WO2005034624A2 (en) | 2003-05-21 | 2005-04-21 | The General Hospital Corporation | Microfabricated compositions and processes for engineering tissues containing multiple cell types |
JP2007526017A (en) | 2003-06-25 | 2007-09-13 | エイセル インコーポレイテッド | Matrix composition tailored for tissue repair |
DE10333863A1 (en) * | 2003-07-24 | 2005-02-17 | Walles, Thorsten Dr. Med. | Bioartificial, primarily vascularized test tissue for pharmacological test methods, process for its preparation and test methods using it, and bioreactor for carrying out the methods |
DK1699915T3 (en) * | 2003-12-30 | 2010-09-13 | Augustinus Bader | Use of erythropoietin for liver tissue regeneration |
EP1550715A1 (en) * | 2003-12-30 | 2005-07-06 | Bionethos Holding Gmbh | Method for the regeneration of tissue |
JP2007051065A (en) * | 2004-02-06 | 2007-03-01 | Tetsuhiro Kitamura | Method and apparatus for regenerating organ |
WO2005085429A1 (en) | 2004-03-05 | 2005-09-15 | Millenium Biologix Inc. | Reverse-flow perfusioin of three-dimensional scaffolds |
US7775965B2 (en) * | 2004-03-09 | 2010-08-17 | The Board Of Regents Of The University Of Oklahoma | Decellularized grafts from umbilical cord vessels and process for preparing and using same |
US20060275270A1 (en) * | 2004-04-28 | 2006-12-07 | Warren William L | In vitro mucosal tissue equivalent |
US7855074B2 (en) * | 2004-04-28 | 2010-12-21 | Vaxdesign Corp. | Artificial immune system: methods for making and use |
US20070141552A1 (en) * | 2004-04-28 | 2007-06-21 | Warren William L | Automatable artificial immune system (AIS) |
US8071373B2 (en) * | 2004-04-28 | 2011-12-06 | Sanofi Pasteur Vaxdesign Corp. | Co-culture lymphoid tissue equivalent (LTE) for an artificial immune system (AIS) |
US7785883B2 (en) * | 2004-04-28 | 2010-08-31 | Vax Design Corp. | Automatable artificial immune system (AIS) |
US8298824B2 (en) | 2004-04-28 | 2012-10-30 | Sanofi Pasteur Vaxdesign Corporation | Methods of evaluating a test agent in a diseased cell model |
US7785806B2 (en) * | 2004-04-28 | 2010-08-31 | Vaxdesign Corporation | Method for determining the immunogenicity of an antigen |
US7709256B2 (en) * | 2004-04-28 | 2010-05-04 | Vaxdesign Corp. | Disease model incorporation into an artificial immune system (AIS) |
US7771999B2 (en) * | 2004-04-28 | 2010-08-10 | Vaxdesign Corp. | Disease model incorporation into an artificial immune system (AIS) |
US8030070B2 (en) * | 2004-04-28 | 2011-10-04 | Sanofi Pasteur Vaxdesign Corp. | Artificial lymphoid tissue equivalent |
US7759120B2 (en) * | 2005-03-02 | 2010-07-20 | Kps Bay Medical, Inc. | Seeding implantable medical devices with cells |
US20060199265A1 (en) * | 2005-03-02 | 2006-09-07 | Wolf Michael F | Seeding implantable medical devices with cells |
US9493735B2 (en) | 2005-04-15 | 2016-11-15 | Wake Forest University Health Sciences | Bioreactor system and method of enhancing functionality of muscle cultured in vitro |
AU2006265601A1 (en) * | 2005-06-30 | 2007-01-11 | Anthrogenesis Corporation | Repair of tympanic membrane using placenta derived collagen biofabric |
WO2007009036A2 (en) * | 2005-07-12 | 2007-01-18 | Tissue Genesis, Inc. | Apparatus and methods for preparing tissue grafts |
US20100093066A1 (en) * | 2005-08-26 | 2010-04-15 | Regents Of The University Of Minnesota | Decellularization and recellularization apparatuses and systems containing the same |
JP2009505752A (en) | 2005-08-26 | 2009-02-12 | リージェンツ オブ ザ ユニバーシティ オブ ミネソタ | Decellularization and recellularization of organs and tissues |
JP2007145844A (en) * | 2005-11-28 | 2007-06-14 | Lifescan Inc | Compositions and methods to create vascularized environment for cellular transplantation |
JP5622358B2 (en) | 2005-12-21 | 2014-11-12 | サノフィ パスツール ヴァックスデザインコーポレーション | in vitro germinal center |
CA2847310A1 (en) * | 2005-12-21 | 2007-07-05 | Sanofi Pasteur Vaxdesign Corporation | A porous membrane device that promotes the differentiation of monocytes into dendritic cells |
EP2409715A1 (en) * | 2006-06-27 | 2012-01-25 | Sanofi Pasteur VaxDesign Corporation | Models for vaccine assessment |
WO2008021391A1 (en) * | 2006-08-15 | 2008-02-21 | Anthrogenesis Corporation | Umbilical cord biomaterial for medical use |
US8071135B2 (en) | 2006-10-04 | 2011-12-06 | Anthrogenesis Corporation | Placental tissue compositions |
KR20230146124A (en) | 2006-10-06 | 2023-10-18 | 셀룰래리티 인코포레이티드 | Native(telopeptide) Placental Collagen Compositions |
US9127254B2 (en) * | 2006-12-18 | 2015-09-08 | Ben-Gurion University Of The Negev Research And Development Authority | Scaffolding for tissue regeneration or repair |
AU2008260187A1 (en) | 2007-05-29 | 2008-12-11 | Christopher B. Reid | Methods for production and uses of multipotent cell populations |
ITVI20070159A1 (en) * | 2007-05-31 | 2008-12-01 | Maurizio Marzaro | METHOD OF PREPARATION FOR THE REVITALIZATION OF AN ORGANIC A-CELLULAR FABRIC AND DEVICE FOR IMPLEMENTING THIS METHOD |
CA2688265C (en) | 2007-06-08 | 2021-02-16 | Wake Forest University Health Sciences | Selective cell therapy for the treatment of renal failure |
WO2009048661A1 (en) * | 2007-07-16 | 2009-04-16 | Vaxdesign Corporation | Artificial tissue constructs comprising alveolar cells and methods for using the same |
US9744043B2 (en) | 2007-07-16 | 2017-08-29 | Lifenet Health | Crafting of cartilage |
US11051733B2 (en) * | 2008-01-18 | 2021-07-06 | Wake Forest University Health Sciences | Isolating and purifying cells for therapy |
US9198733B2 (en) | 2008-04-29 | 2015-12-01 | Virginia Tech Intellectual Properties, Inc. | Treatment planning for electroporation-based therapies |
US10117707B2 (en) | 2008-04-29 | 2018-11-06 | Virginia Tech Intellectual Properties, Inc. | System and method for estimating tissue heating of a target ablation zone for electrical-energy based therapies |
US10702326B2 (en) | 2011-07-15 | 2020-07-07 | Virginia Tech Intellectual Properties, Inc. | Device and method for electroporation based treatment of stenosis of a tubular body part |
US10238447B2 (en) | 2008-04-29 | 2019-03-26 | Virginia Tech Intellectual Properties, Inc. | System and method for ablating a tissue site by electroporation with real-time monitoring of treatment progress |
US8992517B2 (en) | 2008-04-29 | 2015-03-31 | Virginia Tech Intellectual Properties Inc. | Irreversible electroporation to treat aberrant cell masses |
US11272979B2 (en) | 2008-04-29 | 2022-03-15 | Virginia Tech Intellectual Properties, Inc. | System and method for estimating tissue heating of a target ablation zone for electrical-energy based therapies |
US9598691B2 (en) | 2008-04-29 | 2017-03-21 | Virginia Tech Intellectual Properties, Inc. | Irreversible electroporation to create tissue scaffolds |
US9867652B2 (en) | 2008-04-29 | 2018-01-16 | Virginia Tech Intellectual Properties, Inc. | Irreversible electroporation using tissue vasculature to treat aberrant cell masses or create tissue scaffolds |
US11254926B2 (en) | 2008-04-29 | 2022-02-22 | Virginia Tech Intellectual Properties, Inc. | Devices and methods for high frequency electroporation |
US9283051B2 (en) | 2008-04-29 | 2016-03-15 | Virginia Tech Intellectual Properties, Inc. | System and method for estimating a treatment volume for administering electrical-energy based therapies |
US10272178B2 (en) | 2008-04-29 | 2019-04-30 | Virginia Tech Intellectual Properties Inc. | Methods for blood-brain barrier disruption using electrical energy |
US10245098B2 (en) | 2008-04-29 | 2019-04-02 | Virginia Tech Intellectual Properties, Inc. | Acute blood-brain barrier disruption using electrical energy based therapy |
WO2009152384A1 (en) * | 2008-06-11 | 2009-12-17 | The Children's Mercy Hospital | Solutions for tissue engineering and methods of use |
CN102131489A (en) * | 2008-08-20 | 2011-07-20 | 株式会社器官再生工学 | Method for restoring missing tooth and method for producing restorative material |
WO2010057015A1 (en) | 2008-11-14 | 2010-05-20 | Wake Forest University Health Sciences | Kidney structures and methods of forming the same |
WO2010057013A1 (en) | 2008-11-14 | 2010-05-20 | Wake Forest University Health Sciences | Selective cell therapy for the treatment of renal failure |
DK2379122T3 (en) * | 2008-12-24 | 2015-08-03 | Augustinus Bader | FAST PRODUCTION AND USE OF genetically engineered CELLS AND STRUCTURES AS INDIVIDUAL IMPLANTS |
IL196820A0 (en) * | 2009-02-01 | 2009-11-18 | Yissum Res Dev Co | Devitalized, acellular scaffold matrices derived from micro-organs seeded with various cells |
SG174969A1 (en) * | 2009-03-31 | 2011-11-28 | Univ Minnesota | Decellularization and recellularization of organs and tissues |
US11382681B2 (en) | 2009-04-09 | 2022-07-12 | Virginia Tech Intellectual Properties, Inc. | Device and methods for delivery of high frequency electrical pulses for non-thermal ablation |
US11638603B2 (en) | 2009-04-09 | 2023-05-02 | Virginia Tech Intellectual Properties, Inc. | Selective modulation of intracellular effects of cells using pulsed electric fields |
WO2010118387A1 (en) | 2009-04-09 | 2010-10-14 | Virginia Tech Intellectual Properties, Inc. | Integration of very short electric pulses for minimally to noninvasive electroporation |
WO2010138919A2 (en) | 2009-05-28 | 2010-12-02 | Angiodynamics, Inc. | System and method for synchronizing energy delivery to the cardiac rhythm |
ES2599828T3 (en) | 2009-06-04 | 2017-02-03 | The General Hospital Corporation | Bioartificial lung |
US9895189B2 (en) | 2009-06-19 | 2018-02-20 | Angiodynamics, Inc. | Methods of sterilization and treating infection using irreversible electroporation |
US20130323708A1 (en) * | 2009-07-01 | 2013-12-05 | Massachusetts Institute Of Technology | Isolated adult cells, artificial organs, rehabilitated organs, research tools, organ encasements, organ perfusion systems, and methods for preparing and utilizing the same |
US8298586B2 (en) | 2009-07-22 | 2012-10-30 | Acell Inc | Variable density tissue graft composition |
US8652500B2 (en) | 2009-07-22 | 2014-02-18 | Acell, Inc. | Particulate tissue graft with components of differing density and methods of making and using the same |
WO2011059808A2 (en) * | 2009-10-29 | 2011-05-19 | The General Hospital Corporation | Methods for generating pancreatic tissue |
WO2011140241A2 (en) | 2010-05-04 | 2011-11-10 | The General Hospital Corporation | Methods and compositions for preserving tissues and organs |
EP2588592B1 (en) * | 2010-06-30 | 2017-09-06 | Miromatrix Medical Inc. | Use of perfusion decellularized organs for matched recellularization |
EP2603580B1 (en) | 2010-08-13 | 2017-04-12 | Wake Forest University Health Sciences | Methods for making a tissue engineered muscle repair (temr) construct in vitro for implantation in vivo |
ES2562707T3 (en) | 2010-09-01 | 2016-03-07 | Regents Of The University Of Minnesota | Methods of recelularization of a tissue or organ for a better transplant capacity |
EP2625577B1 (en) | 2010-10-08 | 2019-06-26 | Terumo BCT, Inc. | Customizable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system |
WO2012051433A2 (en) | 2010-10-13 | 2012-04-19 | Angiodynamics, Inc. | System and method for electrically ablating tissue of a patient |
KR102238108B1 (en) | 2010-11-10 | 2021-04-12 | 인리젠 | Injectable formulations for organ augmentation |
WO2012088149A2 (en) | 2010-12-20 | 2012-06-28 | Virginia Tech Intellectual Properties, Inc. | High-frequency electroporation for cancer therapy |
EP2670426B1 (en) | 2011-01-31 | 2017-05-10 | The General Hospital Corporation | Multimodal trail molecules and uses in cellular therapies |
KR102034496B1 (en) * | 2011-05-27 | 2019-10-22 | 디퍼이 신테스 프로덕츠, 인코포레이티드 | Bioartificial proximal tubule systems and methods of use |
RU2519326C2 (en) | 2011-12-29 | 2014-06-10 | Общество с ограниченной ответственностью "НекстГен" | Biocomposite for performing reparative processes following injuries in mammals, methods for preparing (versions) and using same |
US9290738B2 (en) | 2012-06-13 | 2016-03-22 | Miromatrix Medical Inc. | Methods of decellularizing bone |
WO2014035474A1 (en) | 2012-08-30 | 2014-03-06 | The General Hospital Corporation | Compositions and methods for treating cancer |
KR102577616B1 (en) | 2013-03-15 | 2023-09-11 | 미로매트릭스 메디칼 인크. | Use of perfusion decellularized liver for islet cell recellularization |
PL3649961T3 (en) | 2013-03-15 | 2021-09-27 | Amniotics Ab | Apparatuses for amniotic fluid collection |
SG11201507515YA (en) | 2013-03-15 | 2015-10-29 | Marcus Kare Torleif Larsson | Cells, methods and apparatuses for umbilical cord blood collection and isolation of cells |
US20150037434A1 (en) * | 2013-08-02 | 2015-02-05 | The Trustees Of Columbia University In The City Of New York | Biomaterials derived from tissue extracellular matrix |
CN103432627B (en) | 2013-08-26 | 2015-03-25 | 北京瑞健高科生物科技有限公司 | Method for preparing animal acellular tissue matrix material and tissue matrix material prepared by same |
US9878071B2 (en) | 2013-10-16 | 2018-01-30 | Purdue Research Foundation | Collagen compositions and methods of use |
WO2015073913A1 (en) | 2013-11-16 | 2015-05-21 | Terumo Bct, Inc. | Expanding cells in a bioreactor |
US10918102B2 (en) | 2014-03-13 | 2021-02-16 | The General Hospital Corporation | Devices and methods to improve and assess viability of human livers |
EP3122866B1 (en) | 2014-03-25 | 2019-11-20 | Terumo BCT, Inc. | Passive replacement of media |
AU2015259303B2 (en) | 2014-05-12 | 2021-10-28 | Arena, Christopher B. | Selective modulation of intracellular effects of cells using pulsed electric fields |
CA2950353C (en) * | 2014-05-26 | 2022-11-01 | Universitatsspital Basel | In vitro culturing or expanding human or animal tissue |
US20160090569A1 (en) | 2014-09-26 | 2016-03-31 | Terumo Bct, Inc. | Scheduled Feed |
US10694972B2 (en) | 2014-12-15 | 2020-06-30 | Virginia Tech Intellectual Properties, Inc. | Devices, systems, and methods for real-time monitoring of electrophysical effects during tissue treatment |
US20180112006A1 (en) | 2015-04-17 | 2018-04-26 | The General Hospital Corporation | Agents, systems and methods for treating cancer |
US11919941B2 (en) | 2015-04-21 | 2024-03-05 | Purdue Research Foundation | Cell-collagen-silica composites and methods of making and using the same |
WO2017004592A1 (en) | 2015-07-02 | 2017-01-05 | Terumo Bct, Inc. | Cell growth with mechanical stimuli |
KR20180104646A (en) | 2016-01-07 | 2018-09-21 | 비.지. 네게브 테크놀로지즈 앤드 애플리케이션스 리미티드, 엣 벤-구리온 유니버시티 | Compositions and methods for establishing immune tolerance |
US20170369849A1 (en) * | 2016-05-11 | 2017-12-28 | The University Of North Carolina At Chapel Hill | Compositions and methods for bioengineered tissues |
US10624992B2 (en) | 2016-05-16 | 2020-04-21 | The General Hospital Corporation | Human airway stem cells in lung epithelial engineering |
US11685883B2 (en) | 2016-06-07 | 2023-06-27 | Terumo Bct, Inc. | Methods and systems for coating a cell growth surface |
US11104874B2 (en) | 2016-06-07 | 2021-08-31 | Terumo Bct, Inc. | Coating a bioreactor |
CN109715140B (en) | 2016-06-15 | 2022-06-24 | 通用医疗公司 | Metabolic labeling and molecular enhancement of biological materials using bio-orthogonal reactions |
US11278643B2 (en) | 2016-09-06 | 2022-03-22 | Mayo Foundation For Medical Education And Research | Use of resected liver serum for whole liver-engineering |
KR102282805B1 (en) * | 2016-09-27 | 2021-07-27 | 후지필름 가부시키가이샤 | Method for manufacturing cell tissue, and porous film |
WO2018073615A1 (en) | 2016-10-21 | 2018-04-26 | Longboat Explorers Ab | Methods and compositions for generating hematopoietic cells |
US10905492B2 (en) | 2016-11-17 | 2021-02-02 | Angiodynamics, Inc. | Techniques for irreversible electroporation using a single-pole tine-style internal device communicating with an external surface electrode |
JP7393945B2 (en) | 2017-03-31 | 2023-12-07 | テルモ ビーシーティー、インコーポレーテッド | cell proliferation |
US11624046B2 (en) | 2017-03-31 | 2023-04-11 | Terumo Bct, Inc. | Cell expansion |
AU2018243725A1 (en) * | 2017-03-31 | 2019-10-17 | Prokidney | Injectable cell and scaffold compositions |
US11739291B2 (en) | 2017-04-25 | 2023-08-29 | Purdue Research Foundation | 3-dimensional (3D) tissue-engineered muscle for tissue restoration |
US11607537B2 (en) | 2017-12-05 | 2023-03-21 | Virginia Tech Intellectual Properties, Inc. | Method for treating neurological disorders, including tumors, with electroporation |
US11925405B2 (en) | 2018-03-13 | 2024-03-12 | Virginia Tech Intellectual Properties, Inc. | Treatment planning system for immunotherapy enhancement via non-thermal ablation |
US11311329B2 (en) | 2018-03-13 | 2022-04-26 | Virginia Tech Intellectual Properties, Inc. | Treatment planning for immunotherapy based treatments using non-thermal ablation techniques |
US11950835B2 (en) | 2019-06-28 | 2024-04-09 | Virginia Tech Intellectual Properties, Inc. | Cycled pulsing to mitigate thermal damage for multi-electrode irreversible electroporation therapy |
EP3886880B1 (en) | 2019-10-18 | 2023-06-07 | Amniotics AB | Processes and apparatuses for obtaining amniotic mesenchymal stem cells from amniotic fluid and cells derived thereof |
Family Cites Families (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4458678A (en) | 1981-10-26 | 1984-07-10 | Massachusetts Institute Of Technology | Cell-seeding procedures involving fibrous lattices |
US4520821A (en) | 1982-04-30 | 1985-06-04 | The Regents Of The University Of California | Growing of long-term biological tissue correction structures in vivo |
US5160490A (en) | 1986-04-18 | 1992-11-03 | Marrow-Tech Incorporated | Three-dimensional cell and tissue culture apparatus |
US5863531A (en) | 1986-04-18 | 1999-01-26 | Advanced Tissue Sciences, Inc. | In vitro preparation of tubular tissue structures by stromal cell culture on a three-dimensional framework |
US5266480A (en) | 1986-04-18 | 1993-11-30 | Advanced Tissue Sciences, Inc. | Three-dimensional skin culture system |
US5032508A (en) * | 1988-09-08 | 1991-07-16 | Marrow-Tech, Inc. | Three-dimensional cell and tissue culture system |
US4963489A (en) | 1987-04-14 | 1990-10-16 | Marrow-Tech, Inc. | Three-dimensional cell and tissue culture system |
US5902741A (en) | 1986-04-18 | 1999-05-11 | Advanced Tissue Sciences, Inc. | Three-dimensional cartilage cultures |
CA1340581C (en) | 1986-11-20 | 1999-06-08 | Joseph P. Vacanti | Chimeric neomorphogenesis of organs by controlled cellular implantation using artificial matrices |
US5567612A (en) | 1986-11-20 | 1996-10-22 | Massachusetts Institute Of Technology | Genitourinary cell-matrix structure for implantation into a human and a method of making |
US5804178A (en) | 1986-11-20 | 1998-09-08 | Massachusetts Institute Of Technology | Implantation of cell-matrix structure adjacent mesentery, omentum or peritoneum tissue |
WO1989001967A1 (en) | 1987-09-03 | 1989-03-09 | Brown University Research Foundation | Blood purification with cultured renal cells |
WO1993007913A1 (en) | 1991-10-24 | 1993-04-29 | Children's Medical Center Corporation | Neomorphogenesis of urological structures in vivo from cell culture |
US5549674A (en) | 1992-03-02 | 1996-08-27 | The Regents Of The University Of Michigan | Methods and compositions of a bioartificial kidney suitable for use in vivo or ex vivo |
US5429938A (en) | 1992-03-02 | 1995-07-04 | University Of Michigan | Methods and compositions for isolation and growth of kidney tubule stem cells, in vitro kidney tubulogenesis and ex vivo construction of renal tubules |
US5441870A (en) * | 1992-04-15 | 1995-08-15 | Athena Neurosciences, Inc. | Methods for monitoring cellular processing of β-amyloid precursor protein |
US5800537A (en) * | 1992-08-07 | 1998-09-01 | Tissue Engineering, Inc. | Method and construct for producing graft tissue from an extracellular matrix |
US5827641A (en) * | 1992-11-13 | 1998-10-27 | Parenteau; Nancy L. | In vitro cornea equivalent model |
US5916265A (en) | 1994-03-30 | 1999-06-29 | Hu; Jie | Method of producing a biological extracellular matrix for use as a cell seeding scaffold and implant |
US5695996A (en) | 1994-09-23 | 1997-12-09 | The United States Of America As Represented By The Department Of Health And Human Services | Artificial organ culture system |
US5855610A (en) | 1995-05-19 | 1999-01-05 | Children's Medical Center Corporation | Engineering of strong, pliable tissues |
AU4331897A (en) | 1996-09-05 | 1998-03-26 | Children's Medical Center Corporation | Prosthetic kidney and its use for treating kidney disease |
JP2001520542A (en) | 1997-04-11 | 2001-10-30 | クライオライフ・インコーポレーテッド | Tissue decellularization |
ES2175753T3 (en) * | 1997-06-27 | 2002-11-16 | Augustinus Bader | BIOSYNTHETIC GRAFT AND METHOD FOR PRODUCTION. |
-
1999
- 1999-12-29 US US09/474,525 patent/US6479064B1/en not_active Expired - Lifetime
-
2000
- 2000-12-14 DE DE60037909T patent/DE60037909T2/en not_active Expired - Lifetime
- 2000-12-14 WO PCT/US2000/033891 patent/WO2001048153A1/en active IP Right Grant
- 2000-12-14 CA CA002395698A patent/CA2395698C/en not_active Expired - Lifetime
- 2000-12-14 EP EP00984372A patent/EP1246903B1/en not_active Expired - Lifetime
- 2000-12-14 JP JP2001548666A patent/JP2003518517A/en active Pending
- 2000-12-14 AU AU20999/01A patent/AU770887B2/en not_active Expired
- 2000-12-14 AT AT00984372T patent/ATE384782T1/en not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
DE60037909D1 (en) | 2008-03-13 |
ATE384782T1 (en) | 2008-02-15 |
DE60037909T2 (en) | 2009-01-15 |
JP2003518517A (en) | 2003-06-10 |
US6479064B1 (en) | 2002-11-12 |
AU770887B2 (en) | 2004-03-04 |
AU2099901A (en) | 2001-07-09 |
EP1246903A1 (en) | 2002-10-09 |
EP1246903B1 (en) | 2008-01-23 |
WO2001048153A1 (en) | 2001-07-05 |
CA2395698C (en) | 2008-01-29 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CA2395698A1 (en) | Reconstructing organs from decellularized biomaterial scaffold | |
US6485723B1 (en) | Enhanced submucosal tissue graft constructs | |
Puelacher et al. | Tissue-engineered growth of cartilage: the effect of varying the concentration of chondrocytes seeded onto synthetic polymer matrices | |
CA2536923C (en) | Extracellular matrix composite materials, and manufacture and use thereof | |
CA2185447A1 (en) | Treated tissue for implantation and preparation methods | |
Auger et al. | A truly new approach for tissue engineering: the LOEX self-assembly technique | |
ES2004281A6 (en) | Method of treating a synthetic naturally occurring surface with a collagen laminate to support microvascular endothelial cell growth, and the surface itself | |
Redlich et al. | Bone engineering on the basis of periosteal cells cultured in polymer fleeces | |
ATE113850T1 (en) | COATING FOR PROSTHESES. | |
MXPA05012927A (en) | Matrix, cell implantation and method for their production and use. | |
JP3865635B2 (en) | Collagen-based supports for tissue engineering and biomaterial manufacturing | |
AU766719B2 (en) | Enhanced submucosal tissue graft constructs | |
Danielsson et al. | Modified collagen fleece, a scaffold for transplantation of human bladder smooth muscle cells | |
WO2002024244A3 (en) | Method of preparing and processing transplant tissue | |
JP2003532466A5 (en) | ||
Grenier et al. | Where to now... that we have tissue engineered blood vessels? Tissue engineered blood vessels! heavens, what will they think of next! | |
Grenier et al. | The self-assembly approach for organ reconstruction by tissue engineering | |
US20080187599A1 (en) | Method and Apparatus for Pressure Sodding a Biological Vascular Conduit | |
Sims et al. | Corrosion casting in the reproduction of the micro-surface topography of fibrillar collagen | |
Damour et al. | Skin equivalents | |
Hörl et al. | Prefabricated free flaps with vascularized porous polytetrafluoroethylene (Proplast II) | |
Lauer | Mucosa tissue engineering | |
Wilczek et al. | Badania kliniczne i doświadczalne w chorobach serca, płuc i naczyń Tissue engineering methods as a potential instrument used in treatment of circulatory system diseases | |
Chen et al. | A TISSUE ENGINEERED PATCH FOR ARTICULAR CARTILAGE REPAIR |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKEX | Expiry |
Effective date: 20201214 |