CA2397545A1 - Method of detecting dna by dna hybridization method with the use of fluorescent resonance energy transfer - Google Patents

Method of detecting dna by dna hybridization method with the use of fluorescent resonance energy transfer Download PDF

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Publication number
CA2397545A1
CA2397545A1 CA002397545A CA2397545A CA2397545A1 CA 2397545 A1 CA2397545 A1 CA 2397545A1 CA 002397545 A CA002397545 A CA 002397545A CA 2397545 A CA2397545 A CA 2397545A CA 2397545 A1 CA2397545 A1 CA 2397545A1
Authority
CA
Canada
Prior art keywords
dna
energy transfer
resonance energy
luminescence
fluorescent resonance
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002397545A
Other languages
French (fr)
Other versions
CA2397545C (en
Inventor
Kazuko Matsumoto
Shinji Sueda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Japan Science and Technology Agency
Original Assignee
Japan Science And Technology Corporation
Kazuko Matsumoto
Shinji Sueda
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japan Science And Technology Corporation, Kazuko Matsumoto, Shinji Sueda filed Critical Japan Science And Technology Corporation
Publication of CA2397545A1 publication Critical patent/CA2397545A1/en
Application granted granted Critical
Publication of CA2397545C publication Critical patent/CA2397545C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
    • C07D209/04—Indoles; Hydrogenated indoles
    • C07D209/08—Indoles; Hydrogenated indoles with only hydrogen atoms or radicals containing only hydrogen and carbon atoms, directly attached to carbon atoms of the hetero ring
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Abstract

A practically usable DNA detection method by a DNA hybridization method with the use of fluorescent resonance energy transfer wherein the background luminescence (i.e., luminescence based on the luminescence of a donor and the direct excitation of an acceptor) affecting the sensitized luminescence of the acceptor is minimized so that a target DNA strand can be conveniently and very easily detected in a homogeneous solution. In this DNA detection method, a DNA is detected by the DNA
hybridization method by using, as detection reagents, streptoavidin labeled with a fluorescent rare earth metal complex, a nucleic acid probe modified with biotin and a nucleic acid probe labeled with an organic cyanine pigment and taking advantage of fluorescent resonance energy transfer.
CA002397545A 2000-01-20 2000-07-26 Method of detecting dna by dna hybridization method with the use of fluorescent resonance energy transfer Expired - Fee Related CA2397545C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2000011771A JP2001204497A (en) 2000-01-20 2000-01-20 Method for detecting dna by dna hybridization method by utilizing transfer of fluorescence resonance energy
JP2000-011771 2000-01-20
PCT/JP2000/004975 WO2001053527A1 (en) 2000-01-20 2000-07-26 Method of detecting dna by dna hybridization method with the use of fluorescent resonance energy transfer

Publications (2)

Publication Number Publication Date
CA2397545A1 true CA2397545A1 (en) 2001-07-26
CA2397545C CA2397545C (en) 2007-04-03

Family

ID=18539595

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002397545A Expired - Fee Related CA2397545C (en) 2000-01-20 2000-07-26 Method of detecting dna by dna hybridization method with the use of fluorescent resonance energy transfer

Country Status (4)

Country Link
US (1) US6830889B1 (en)
JP (1) JP2001204497A (en)
CA (1) CA2397545C (en)
WO (1) WO2001053527A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113985038A (en) * 2021-10-19 2022-01-28 福州市第二医院（福建省福州中西医结合医院、福州市职业病医院） Biosensor and preparation method and application thereof

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EP1349954B1 (en) * 2000-05-19 2011-01-26 Eragen Biosciences, Inc. Materials and methods for detection of nucleic acids
AU1317502A (en) * 2000-10-14 2002-04-29 Eragen Biosciences Inc Solid support assay systems and methods utilizing non-standard bases
US7033819B2 (en) 2000-11-08 2006-04-25 Surface Logix, Inc. System for monitoring cell motility in real-time
US7033821B2 (en) 2000-11-08 2006-04-25 Surface Logix, Inc. Device for monitoring cell motility in real-time
US6893851B2 (en) 2000-11-08 2005-05-17 Surface Logix, Inc. Method for arraying biomolecules and for monitoring cell motility in real-time
US6864065B2 (en) 2000-11-08 2005-03-08 Surface Logix, Inc. Assays for monitoring cell motility in real-time
CA2490654C (en) * 2002-07-25 2012-02-07 Aclara Biosciences, Inc. Detecting receptor oligomerization
CN1735808A (en) * 2002-11-07 2006-02-15 鹿特丹伊拉兹马斯大学 FRET probes and methods for detecting interacting molecules
KR100581002B1 (en) * 2003-03-25 2006-05-17 바이오코아 주식회사 Oligonucleotide composition for analyzing human leukocyte antigen genotype and detecting method thereof
CN100557028C (en) * 2004-03-24 2009-11-04 博康有限公司 Be used to analyze genotypic oligonucleotide composition of human leucocyte antigen (HLA) and detection method thereof
EP2174115B1 (en) * 2007-07-09 2013-08-28 Fio Corporation Systems and methods for enhancing fluorescent detection of target molecules in a test sample
US8674080B2 (en) * 2009-04-09 2014-03-18 Roche Molecular Systems, Inc. Dye composition for liquid transfer control
CN104204799A (en) * 2012-02-06 2014-12-10 珀金埃尔默生物信号股份有限公司 Dual-acceptor time-resolved-fret
WO2016163345A1 (en) * 2015-04-07 2016-10-13 コニカミノルタ株式会社 Nucleic acid probe
TWI627407B (en) 2015-12-01 2018-06-21 國立交通大學 Water-soluble peptide fluorescence material
CN114858878B (en) * 2022-04-19 2023-10-27 常州大学 Sensor for detecting tetracycline by electrochemical luminescence method based on resonance energy transfer, and preparation method and application thereof

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5556959A (en) * 1993-01-22 1996-09-17 Pharmacia P-L Biochemicals Inc. Indocarbocyanine-linked phosphoramidites
JP3538277B2 (en) * 1996-03-08 2004-06-14 和子 松本 Labeling reagent for immunoassay, fluorescent compound and complex used therefor, and immunoassay for biological substance using them
JP4008996B2 (en) * 1997-12-24 2007-11-14 浜松ホトニクス株式会社 Target nucleic acid detection probe
US6080868A (en) * 1998-01-23 2000-06-27 The Perkin-Elmer Corporation Nitro-substituted non-fluorescent asymmetric cyanine dye compounds
CA2319490A1 (en) * 1998-02-03 1999-08-05 Amersham Pharmacia Biotech Inc. Energy transfer dyes

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113985038A (en) * 2021-10-19 2022-01-28 福州市第二医院（福建省福州中西医结合医院、福州市职业病医院） Biosensor and preparation method and application thereof

Also Published As

Publication number Publication date
JP2001204497A (en) 2001-07-31
US6830889B1 (en) 2004-12-14
CA2397545C (en) 2007-04-03
WO2001053527A1 (en) 2001-07-26

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