CA2407505A1 - Hepatocyte lineage cells derived from pluripotent stem cells - Google Patents

Hepatocyte lineage cells derived from pluripotent stem cells Download PDF

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CA2407505A1
CA2407505A1 CA002407505A CA2407505A CA2407505A1 CA 2407505 A1 CA2407505 A1 CA 2407505A1 CA 002407505 A CA002407505 A CA 002407505A CA 2407505 A CA2407505 A CA 2407505A CA 2407505 A1 CA2407505 A1 CA 2407505A1
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cells
stem cells
pluripotent stem
hepatocytes
butyrate
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CA2407505C (en
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Lakshmi Rambhatla
Melissa K. Carpenter
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Asterias Biotherapeutics Inc
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    • C12N5/067—Hepatocytes
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Abstract

It has been discovered that when pluripotent stem cells are cultured in the presence of a hepatocyte differentiation agent, a population of cells is derived that has a remarkably high proportion of cells with phenotypic characteristics of liver cells. In one example, human embryonic stem cells are allowed to form embryoid bodies, and then combined with the differentiation agent n-butyrate, optionally supplemented with maturation factors. In another example, n-butyrate is added to human embryonic stem cells in feeder-free culture.
Either way, a remarkably uniform cell population is obtained, which is predominated by cells with morphological features of hepatocytes, expressing surface markers characteristic of hepatocytes, and having enzymatic and biosynthetic activity important for liver function. Since stem cells readily proliferate in culture, this system provides an abundant source of cells of the hepatocyte lineage for a variety of applications, such as drug screening, and replenishing liver function in the context of clinical treatment.
CA002407505A 2000-04-27 2001-04-26 Hepatocyte lineage cells derived from pluripotent stem cells Expired - Fee Related CA2407505C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US20009500P 2000-04-27 2000-04-27
US60/200,095 2000-04-27
US09/718,308 US6458589B1 (en) 2000-04-27 2000-11-20 Hepatocyte lineage cells derived from pluripotent stem cells
US09/718,308 2000-11-20
PCT/US2001/013471 WO2001081549A2 (en) 2000-04-27 2001-04-26 Hepatocyte lineage cells derived from pluripotent stem cells

Publications (2)

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CA2407505A1 true CA2407505A1 (en) 2001-11-01
CA2407505C CA2407505C (en) 2007-10-23

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CA002407505A Expired - Fee Related CA2407505C (en) 2000-04-27 2001-04-26 Hepatocyte lineage cells derived from pluripotent stem cells

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US (2) US6458589B1 (en)
EP (2) EP2256187A1 (en)
JP (2) JP5711441B2 (en)
KR (1) KR100729971B1 (en)
CN (2) CN1439049A (en)
AU (3) AU5917001A (en)
CA (1) CA2407505C (en)
GB (1) GB2380490B (en)
IL (2) IL152481A0 (en)
WO (1) WO2001081549A2 (en)

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