CA2433235C - Apparatus and method for filtering biological samples - Google Patents

Apparatus and method for filtering biological samples Download PDF

Info

Publication number
CA2433235C
CA2433235C CA2433235A CA2433235A CA2433235C CA 2433235 C CA2433235 C CA 2433235C CA 2433235 A CA2433235 A CA 2433235A CA 2433235 A CA2433235 A CA 2433235A CA 2433235 C CA2433235 C CA 2433235C
Authority
CA
Canada
Prior art keywords
sample
holder
end portion
connection
filtrate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2433235A
Other languages
French (fr)
Other versions
CA2433235A1 (en
Inventor
Ann Efthimiadis
Donald William Mccormack
Frederick Hargreave
Parameswaran Nair
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CELLOMETRICS Inc
Original Assignee
Ann Efthimiadis
Donald William Mccormack
Frederick Hargreave
Parameswaran Nair
St. Joseph's Healthcare-Hamilton
2158141 Ontario Inc.
Cellometrics Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ann Efthimiadis, Donald William Mccormack, Frederick Hargreave, Parameswaran Nair, St. Joseph's Healthcare-Hamilton, 2158141 Ontario Inc., Cellometrics Inc. filed Critical Ann Efthimiadis
Publication of CA2433235A1 publication Critical patent/CA2433235A1/en
Application granted granted Critical
Publication of CA2433235C publication Critical patent/CA2433235C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/405—Concentrating samples by adsorption or absorption
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
    • A61B10/0045—Devices for taking samples of body liquids
    • A61B10/0051—Devices for taking samples of body liquids for taking saliva or sputum samples
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
    • A61B10/0096—Casings for storing test samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/56—Labware specially adapted for transferring fluids
    • B01L3/563—Joints or fittings ; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2201/00—Details relating to filtering apparatus
    • B01D2201/02—Filtering elements having a conical form
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/043—Hinged closures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/044—Connecting closures to device or container pierceable, e.g. films, membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0851—Bottom walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0638—Valves, specific forms thereof with moving parts membrane valves, flap valves
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Abstract

There is provided an apparatus and method for collection and filtration of a sample having a sample holder, a filtrate holder and a connection conduit having a sample receiving end and a filtrate receiving end and containing a filter. The sample holder has an entry end portion and an exit end portion, with a diaphragm normally closing the exit end portion. The filtrate holder has a closeable receiving end portion for receiving the filtered sample. The connection conduit has a rupturing device for rupturing the diaphragm on exit end portion of the sample holder. When the sample holder is inserted into the connection conduit, the diaphragm rupturing device ruptures the diaphragm, allowing the sample be filtered by the filter located within the connection conduit and the filtrate is collected in the filtrate holder.
Also described is a method of collecting a biological sample, and filtering the sample, and holding the filtrate in a filtrate holder.

Description

APPARATUS AND METHOD FOR FILTERING BIOLOGICAL SAMPLES
BACI(GROUND OF THE INVENTION
1. Field of the Invention The present invention relates to a system of retricwing and analysing biological samples that improves the accuracy and reproducibility of results ~btained from analysis of mucoid andlor suspension samples. The present invention provides means of promoting standardization of sample collection and analysis between clinical studies and between clinical centres. More particularly, this invention relates to a two vial interconnected filtration system, that allows samples to pass from a sample tube to a filtrate tube, through standardized filters and to be treated with standardized reagents for analysis.
2. Description of the Prior Art Collection and analysis of biological samples have been valuable for correct diagnosis and therapy of a wide variety of different conditions. In particular, airway inflammation, which is present in many diseases including asthma, chronic obstructive pulmonary disease and lower respiratory tract infections, may be detected from analysis of sputum. Sputum is defined as the expectorated lower respiratory secretions and is composed of fluid and cellular components including eosinophils, neutrophils, lymphocytes, macrophages, and epithelial cells.
Analysis of the cells present in sputum is the best, presently known way to characterise the severity and type of inflammation present. This information in conjunction with other clinical parameters may be used to determine specific courses of treatment.
Increases in certain types of cells may indicate an infection or an inflammation that would be more sensitive to one course of treatment as opposed to another.
Hence, it is important that the collection, processing and analysis of sputum, aspirate, or other biological fluids, are as accurate as possible.
Presently, methodology used for collecting, processing, frltering and analysing biological samples, such as sputum, vary from lab to lab. Variations in technique will cause variations in the results obtained during analysis such as, detection of numbers of cells and fluid phase indices. This becomes important in relation to reproducibility, validity, responsiveness and comparison of results -within and between samples. If the results cannot be compared between tests, or if the results of a test cannot be reproduced, valid diagnosis and accurate courses of treatment cannot be determined. Procedures and the equipment used must therefore be standardised.
An important part of the process for analysis of certain biological samples, is the filtration of the collected sample and the collection of the filtrate containing the single cell or cellular suspension that the technician wishes to analyse.
Variation in the protocol for processing samples for anal!~OSis is currently one area that causes variation in results between tests and between testing centres.
The variation in results is largely due to of the wide variability in protocols, reagents, and materials currently used for the collection and analysis of samples.
Vllhen dealing with limited quantities of samples for analysis, even a minute loss of sample could drastically alter the results of a test. The size, number and type of cells found in the filtrate vary depending on the size and type of filter used.
Incorrect filter pore size may result in selective cell loss and incorrect cell differentials: Incorrect filter mesh type and size may result in cell loss due to absorption of small samples. Improper placement of the filter may allow the fil-trate to become contaminated by debris. Contaminates or debris may cause problems in analysis by varying the results that are produced, or by clogging apertures of automated cell counters. In addition to this, each time that a sample is handled and transferred from one vessel to another there will be loss of sample volume and content causing errors in the analysis and the final results of the experiment.
Selection of the filter type and size necessary for remove( of undesirable contaminants and debris is an important component that contributes to reproducible and valid results.
There is a need for a system that will standardize the protocol used by various technicians and labs so that results may be cross-referenced and prepared for diagnostic purposes between samples, befi~veen tests and between laboratories. Reasons for comparing test results between samples would be, for example, if a patient is being treated by a certain type of drug it is dEairable to know if the prescribed drug is having an effect on the condition present.
Samples may be collected at various points during a course of treatment and comparison of measurements such as cell counts andlor fluid phase indices may be analysed to determine effect of treatment. Clinical treatment may then be adjusted accordingly. Lack of standardized tests complicate comparison of analysis results of different samples, which may make it impossible i:o make an absolute clinical diagnostic decision, and to monitor treatment. It would rant be definitive if variations in results were due to erroneous pr~ooedures or to actual change as a result of treatment. A system developed with a standardized collection and filtration procedure could eliminate many errors in the handling, examination and measurement of a sample. A system ire which a sample could be collected in a sample holder, transported to the lab and then filtered through an internal filtration conduit into a second receiving vial, would be a desiirable contribution to science. Currently, in most cases, samples are being collected in one collection vessel or sample holder. The sample is then transported to a lab.
The sample is then poured from this vessel onto filters of varying sizes depending on the protocol in use. The filtered sample, or filtrate, is irhen received in a second receiving vessel, or filtrate holder. Transfers of a sample from vessel to vessel many times will cause loss of volume and cell yield.
In accordance with the invention disclosed herein, it will be seen that a device is used for collecting a sample, filtering the samplle through a filter with a standardized mesh size, and receiving the filtered sample directly into another connected vessel. A device capable of performing these steps in a closed system will provide a solution to many of the aforementioned problems. The steps of collection, filtration, collection of filtrate and analysis, could be conducted within one system and would eliminate transfer of sample. This may reduce chances of sample volume being lost, wrong filter sizes or types being used or improper filter positioning, thus preventing debris from passing through into the filtrate causing contamination. This system will use a standardized filter type and pore size, or have various filters available within a kit for specific purposes. The collected 7 5 samples could be treated with reagents, in the collections vial or sample holder if desired. Treating the sample in the collection vial may therefore eliminates transfer steps and reduces sample loss. Use of a standardised system having standard filters, reagents, and apparatuses, would therefore allow all technicians to use the same protocol and result in comparable data and thus be reproducible and valid.
Furthermore, a system that eliminates or greatly reduces human exposure to the sample is greatly desirable in settings where that sample may .contain contagious. 1n accordance with the present invention, the collection or sample holder may be sealed from human contact from the point of collection. Both the receiving or collection vials may have reagents placed in it prior to collection or processing.
A device that could reduce the number of errors in processing a sample, increase the validity, reproducibility and simplify a process would be of great interest to pharmaceutical companies, hospitals, and research laboratories.
There could be a decrease in the number of man-hours necessary and a reduction i~
the amount of reagents and equipment used for analysis, therefore a reduction in costs.
Availability of a standard filtering system along with various standardized filters, and testing reagents in a kit would provide a standardized protocol and be an advantage for producing valid and reproducible result The foregoing and other objects and advantages of the present invention will become more apparent from the following summary and the detailed description of the present invention, when taken in conjunction with the accompanying drawings, the scope of which will be pointed out in the appended claims.
SUMMARY OF THE INVENTION
In accordance with this invention, there is provided an apparatus for collection of a mucoid or suspension sample for filtration which comprises a sample holder, a filtrate holder, and a connection conduit containing a filter, which can be connected between the sample and filtrate holders. The suspension sample passes from the sample holder through the filter, which collects contaminants such as debris, and passes to the filtrate holder as filtrate.
The sample holder has an entry end portion for collecting the mucoid or suspension sample, and an exit end portion. A diaphragm, or a valve, or a perforated disc mechanism seals the exit end. When the sample holder is connected with the connection conduit, a diaphragm rupturing device then ruptures the diaphragm of the sample holder, thus allowing the suspension sample to flow freely from the sample holder to the filter that is contained within the connection conduit.
The suspension sample passes through the filter, purifying the sample by removal of contaminates such as debris. The filtrate is then received in the filtrate holder, which may be removed, sealed and taken to a lab for analysis and pirocesses demanded by the tests that are being performed. The sample and filtrate holders of the filtration system may be inscribed with graduated markings for easy determination of the volume of sample contained therein, thus allowing direct calculation of concentration.
The diaphragm rupturing device, as illustrated in the preferred embodiment, is a male shaped protrusion. The diaphragm of the sample holder is aligned with the male shaped protrusion such that the diaphragm rests on top of the male shaped protrusion. The male shaped protrusion ruptures the diaphragm of the exit end portion of the sample holder when the sample holder and the rupturing device are pushed together, thus forcing the protrusion to puncture the diaphragm. As illustrated by the preferred embodiment, the diaphragm rupturing device is mounted on a support member, which is mounted within the connection conduit.
When the sample holder is connected to the connection conduit, and pressure is applied, the diaphragm is forced against the male shaped protrusiore. As the support member moves closer relative to the connection conduit, in the direction of the applied force, the diaphragm is moved to one side, permitting the sample to flow into the connection conduit. Although the support member is illustrated in the preferred embodiment as fixed in the connection conduit, in another embodiment it could be a mounted on a moveable support member.
The male shaped protrusion defines a pathway a>r conduit through which the suspension sample flows from the sample holder. The pathway is a hollow channel through the male shaped protrusion, and connects the sample holder to an interior chamber in the connection conduit, located between the diaphragm rupturing device and the filter, within the connection conduit. The diaphragm rupturing device could be a variety of members that could cause a rupture or tear in a diaphragm, and is not limited to a male shaped protrusion. The suspension sample could flow directly from the ruptured diaphragm into the connection conduit without the aid of a channel, if desired.
It should be noted that the use of a diaphragm arid a rupturing device is only one method for connecting the sample holder to the filter and the filtrate holder. Other possible means of connection may include a selfsealing elastomeric end, through which a syringe type connector may be inserted, such as the type used for drug vials. Another embodiment may use perforated rotatable disks, such that the perforations on each disk will allow a sample to flow through only when the perforations are aligned with one another. When the perforations are not aligned with one another, the perforations are sealed by the opposing disk.
The connection conduit may also support an inlet , which pasaes Pram the outside of the connection conduit to the interior chamber of the connection conduit. The inlet allows introduction of solutions from outside the filtration system. This would be desirable, to allow introduction of filter wetting solutions, and reagents or the like, without opening the filtration system to the environment, thus preventing risk of spillage, contamination of the sample, ar exposure of the technician to infectious agents.
The interior chamber is defined by the walls of the connectior' conduit, the diaphragm rupturing device and support member, and a filter. Once the diaphragm is ruptured, the suspension sample flows from the sample holder, through the male shaped protrusion channel, into the interior chamber and onto the filter, where it is filtered and collected in the filtrate holder.
BRIEF DESCRIPTION OF THE DRAWINGS
Figure 1 is a perspective illustration of the sample collection device partially exploded;
Figure 2 is a vertical section of a first component, the sample holder, of the sample collection device;
Figure 3 is a vertical section of a second component, the filtrate holder, of the sample collection device;
Figure 4 is a vertical section of a third component, the connection conduit, of the sample collection device;

Figure 5 is a exploded vertical side elevation of the three filter components of the sample collection device;
Figure 6 is a vertical section of some components of the sample holder, shown partially assembled, at a first assembly stage;
Figure 7 is a vertical section corresponding to Fig 6 of all the components showing the collector vial partially inserted into the connection condluit, at a second assembly stage;
Figure 8 is a vertical section corresponding to Fig 7 showing the collection vial fully inserted into the connection conduit, at a third assembly s#a~ge;
and, Figure 9 is an exploded side elevation of the three filter components and portions of the sample holder, the connector conduit and the filtrate holder DESCRIPTION OF THE INVENTION
The invention is illustrated in a preferred embodiment, in the form of a device in which sputum aspirate or other biological suspension samples may be sampled in a three part sampling and filtration system(10), as illustrated in Figures 1 through 9. The filtration system (10) includes a collection or samplle holder (12).
The collection or sample holder(12) for collection of a biological sample, such as a mucoid or suspension sample, has an entry end portion(1~4) and an exit end portion(16). The entry end portion(14) for collecting a suspension sample is closeable and has outer threads(18) allowing cap , stopper, or other closure(20) with attachments such as inner threads(not shown) to be attached to the entry end portion(14) in a well known manner. The exit end portion(16) of the sample holder(12) defines an opening(22) which is temporarily closed, for example in this case, being rupturably sealed with diaphragm(24). The diaphragm(24), typically made of a prophylactic material of a type that is unreactive with the suspension sample collected for filtration, seals the exit end portion(16) and retains the collected sample in the sample holder(12). The sample holder(12) is inscribed with graduated markings,( not shown) for easy determination of sample volume.
Around the diaphragm (24), a sloping ledge (26) is formed inside exit end (16), for reasons to be described below. Diaphragm (24) is mounted on a hinge flap (28), and is normally bonded to ledge (26), (Fig 2), ~,tntil engaged by the rupture device, described below.
The receiving or filtrate holder(30) of the illustrated embodiment, is generally made of the same material as the sample holder(12), and is closed at one end, in this case the lower end, as illustrated. The filtrate holder(30) has an open ended receiving end portion(32) with attachments such as outer threads(34), and is closeable after collection of the filtrate with a cap , stopper, or other closure (36) which may have interior threads(not shown). The filtrate holde:r(30) may also be inscribed with graduated markings for easy determination of volume of filtrate received.
The connection conduit(38) of the illustrated embodiment, has a sample receiving end portion(40) for connection with the exit end portion(16) of the sample hoider(12). In this embodiment, the sample holder(12) has a circumference at its exit end portion(16), which is smaller than the interior circumference of the sample receiving end portion(40) of the connection conduit(38). This permits the exit end {16) of sample holder (12), to make a telescopic sliding fit into the rE;ceiving end (40) of connection conduit (38). However other configurations are possible.
The connection conduit (38) has a lower attachment end {42), which is threaded on its interior and is adapted to be attached to the open or receiving end (32) of filtrate holder 30.
The connection conduit {38), in this errabodiment, is shaped to receive a diaphragm rupturing device(44) on support member(46). The diaphragm rupturing device{44) defines a pathway(48) through which the suspension sample will flow, from the sample holder (12) after the diaphragm(14) on the exit end portion(16) of the sample holder(12) is ruptured. Vacuum release hole(50) passes through wall(52) of the connection conduit(38). Vacuum release hole(50) allows equalization of pressure, which thereby permits the sample to flow after diaphragm(24) is ruptured.
The above described connection between the sample holder(12) and filtrate holder(30) and the connection conduit(38), illustrate only one method of connection. It should be noted that the method of connection is not restricted to the telescopic sliding fit described. ~ther forms on interconnection sore possible The diaphragm rupturing device(44), as illustrated in the preferred embodiment, (Figs. 5 and 9), is a male shaped tubular protrusion(54). This is formed by defining an angled edge (56), at the free end of protrusion (54).
The male shaped protrusion(54) ruptures the diaphragm(24) of the exit.end portion(16) of the sample holder(12) when pressure is applied, forcing the sample holder (12) into the connection conduit (38). As the sample holder (12) slides into connection conduit (38), edge (56) of male shaped protrusion(54) presses against and ruptures the diaphragm(24), thereby swinging the diaphragm 24 inwardly (Fig.
8) about its hinge flap 28, to one side of sample holder (12).
Alternatively, it will be appreciated that the rupture device (44) could be mounted on some form of slide (not shown), which would be manually slidable relative to connection conduit (38). In this case when the sample h~older(12}
was connected to the connection conduit(38), pressure would be applied on the diaphragm(24) by a male shaped protrusion{54) which is moved relative to the axis of connection conduit (38).
The male shaped tubular protrusion(54} defines a pathway or channel(58) through which the suspension sample flows from the sample holder(12), into connection conduit (38). The pathway(58} extends through the male shaped protrusion(54), and connects the sample holder(12) to connection conduit (38).
Within connection conduit (38) there is also received a filter support (60).
Filter support (60) is cylindrical in exterior shape and makes a snug pressure fit within the attachment end (42} of connection conduit (38}. Filter support (60) defines an interior filter support wall (52) of generally frusto-conical shape in section, wider at its upper end and tapering to its lower end. A drainage tube (64) extends downwardly from its lower end. Connection conduit (38) defines at its lower end a flange (66), which functions to retain filter support (60) iin position within connection conduit (38).
A filter media (68) made of suitable, preferably di sposable, filter material, is located within filter support (60). It is of conical shape, ire this embodiment, and is supported by filter support wall (62) (Fig 9). The invention is not restricted to any particular shapes however. Filters, and filter supports could be of any functional shape. The filters could simply be fiat discs in some cases, resting on any suitable frame work (not shown) allowing flow of filtrate from the filter.
Free flow of sample liquid from sample holder (12:), down though filter media (68) is permitted by allowing entry of air through <~ir vent hole (50) provided in wall (52) of connection conduit (38).
In order to ensure complete drainage of sample liquid from sample holder (12) a plurality of drain holes (70) are provided around male tubular protrusion (54). These ensure that any liquid which might collect around the ouitside of male protrusion (54), can drain through such holes (70) and flow down through the filter media (68).
The air inlet (50), which passes from the outside of the connection conduit (38) to the interior also allows introduction of solutions from outside the filtration system. This would be desirable, to allow introduction of filter wetting solutions, reagents or the like, without opening the sample collection system(10) to the environment. This will prevent risk of spillage, contamination of the :>ample or exposure of the technician to infectious agents.

In order to ensure that the sample holder (12) and the connection conduit (38) are guided into the correct relation during telescopiic interconnection, sample holder (12) is formed with an exterior guide ridge (72), and connection conduit (38) is formed with a complementary guide groove (74). When these two are registered and in sliding relation, the sample holder will be guided into the correct rotational orientation relative to the connection conduit (38), to ensure easy rupture of the diaphragm (24) Similarly, the support (46) of the rupture device (~~4) has a guiide block (76) formed thereon, and guide block (76) also fits into guide groove (74;) of connection conduit (38). This ensures that the rupture device (44) is in a preset orientation, when pressed into connection conduit (38), which corresponds with the desired orientation of sample holder (12).
Many methods may be used far capping and sealing the filtrate holder (30), such as a threaded cap, stopper, or other closure for example.
In Operation In operation a physician, technician, nurse, or the like, collects a biological sample or a series of samples in a series of sample holders(12), from the subject source or different subject sources, into the entry end portion(14) of the sample holder(12). At this time the exit end portion(16) of the sample holder~(12) is still sealed by the rupturable diaphragm (24) and may be covered by a protective means (not shown) to prevent accidental rupture of the diaphragm (24). The entry end portion(14) of the sample holder(12) is then capped or sealed by a sealing means, such as cap , stopper, or other closure (20) and the sample holder (12) is transported for testing (As shown in Fig. 2). Bf desirable, reagents rnay be added to the collected sample in the sample holder(12).
At the testing-facility, the exit end portion(16) of the sample holder(12) is attached to the sample receiving end portion(40) of the connection conduit(38) by threads or some other convenient connection means. In the case of this embodiment, the sample holder (12) is then slid into the connection conduit (38) and gradually pressed further into telescopic engagement. This will bring male protrusion (54) into contact with diaphragm (24)(See Figs. 6 and 7).
At a suitable point in the operation, a solution may be introduced through the hole (50), in this embodiment by means of injection, or may be introduced through a resealable injection port (not shown ) of a type weN known in the art, for wetting the filter and/ or mixing with the sample before filtration.
Further relative movement between sample holder {12) and connection conduit (38) will rupture diaphragm (24)(Fig. 8). Once the diaphragm(24) is ruptured, the sample flows from the sample holder(12), through the male shaped tubular protrusion (54), and flows into the connection conduit(38), and onto the filter media (68). There it is filtered and drains though drain tube (64) and flows into the filtrate holder(30). The filtrate holder (30), as illustrated in this embodiment, will already have been attached to the connection conduit (38) by threads (34). When filtration is complete, the filtrate holder(30) may then be removed from the connection conduit(38) and capped, or otherwise closed, and sealed for storage, transport or for testing procedures such as cell counts and centrifugation(Fig. 3).
In this embodiment, as the sample passes through the filter media (68), any debris or contaminates that are larger than the pores (not shown) or mesh size (not shown) of the filter media (68) are removed.
The sample holder(12) and the connection conduit(38), togetller with entire filter assembly and filter media (68) may be discarded, or the parts may be sterilized for future use with a replacement Biter.
Once the filtrate is received in the filtrate holder (30), a closeable cap, stopper, or other closure (not shown), or other form of seal, protects the purified sample or filtrate from becoming contaminated, or lost due to spillage, and also protects technicians and the like from exposure. The closure may be removed when testing of the purified sample begins. After use, the filtrate holder(30) may be sterilized for future use or discarded.
Typically the entire system can be supplied in kit form. A sterile pack (not shown) would contain the sample holder (12), the connection conduit (38), containing the filter support (60}, filter media (68) and rupture devicE=
(44.} already locked in place, and a filtrate holder (30), and suitable cap, stopper, or other closures or other seals, and dosage sized containers of reagents, filter wetting fluids and the like, and possibly gloves for handling the entire system, and a face mask. Such a kit, in pack form, would ensure that as far as possible all samples were taken in the same manner, and were all handled under sterile conditions, and were all filtered to the same degree.
The foregoing is a description of a preferred embodiment of the invention which is given here by way of example only. The invention is not to be taken as limited to any of the specific features as described, but comprehends all such variations thereof as come within the scope of the appended claims.

Claims (41)

1. An apparatus for collection of a suspension sample for filtration comprising:
a sample holder for collecting the suspension sample;
a filtrate holder for receiving the suspension sample;
a connection conduit having a sample holder connection end for connecting to said sample holder, said connection conduit having a filtrate holder connection end for connecting to said filtrate holder, thereby connecting said sample holder to said filtrate holder;
a filter mounted in said connection conduit, whereby the suspension sample passes through said filter before entering said filtrate holder.
2. The apparatus as claimed in claim 1, wherein said sample holder has an entry end portion and an exit end portion.
3. The apparatus as claimed in claim 2, wherein said exit end portion of said sample holder has a diaphragm normally closing said exit end portion.
4. The apparatus as claimed in claim 3, wherein said entry end portion of said sample holder is closeable.
5. The apparatus as claimed in claim , wherein said connection conduit has a rupturing device for rupturing said diaphragm of said exit end portion of said sample holder, thereby allowing the suspension sample to exit said sample holder.
6. The apparatus as claimed in claim 5, wherein said rupturing device for rupturing said diaphragm is a male shaped protrusion, said male shaped protrusion defining a pathway through which the suspension sample exits said sample holder.
7. The apparatus as claimed in claim 6, wherein said male shaped protrusion is tubular and is formed with an angled edge.
8. The apparatus as claimed in claim 7, wherein said connection conduit has a filter support mounted therein.
9. The apparatus as claimed in claim 8, wherein said filtrate holder has a receiving end portion for receiving the filtered suspension sample from said connection conduit.
10. The apparatus as claimed in claim 9, wherein said filtrate holder is closeable at said receiving end portion, after reception of said filtrate.
11. The apparatus as claimed in claim 8, further comprising a filter media supported by said fitter support.
12. The apparatus as claimed in claim 11, wherein said filter media is conical, and wherein said filter holder defines a generally frusto-conical support wall and a drainage tube at the apex thereof.
13. The apparatus as claimed in claim 12, wherein said diaphragm is hingedly mounted to said sample holder
14. The apparatus as claimed in claim 13, further comprising an angled ledge formed around the interior of said sample holder for receiving said diaphragm.
15. The apparatus as claimed in claim 14, wherein said support member is formed with connection guides and wherein said connection conduit is formed with mating guides to guide relative movement therebetween.
16. A method of filtering a suspension sample, comprising the steps of:
collecting a sample in a sample holder, said sample holder having an exit end portion sealed by a closure;
connecting a filtrate holder to said sample holder;

opening said closure;
allowing the suspension sample to flow from said sample holder to a filter and filtering the suspension sample through said filter; and collecting said filtered sample in said filtrate holder.
17. The method of filtering a suspension sample as claimed in claim 16, further comprising the step of collecting the suspension sample at an entry end portion of said sample holder.
18. The method of filtering a suspension sample as claimed in claim 17, further comprising the step of closing said entry end portion of said sample holder.
19. The method of filtering a suspension sample as claimed in claim 18, further comprising the step of connecting said sample holder to a sample receiving end portion of said connection conduit.
20. The method of filtering a suspension sample as claimed in claim 19, further comprising the step of passing a solution through an inlet, whereby said filter is moistened.
21. The method of filtering a suspension sample as claimed in claim 20, further comprising the step of sealing said inlet, thereby preventing loss of the suspension sample.
22. The method of filtering a suspension sample as claimed in claim 21, further comprising the step of rupturing said diaphragm on a male shaped protrusion of said connection conduit.
23. The method of filtering a suspension sample as claimed in claim 22, further comprising the step of closing said filtrate holder by a closing device.
24. A connection apparatus for connecting a sample holder and a filtrate holder and comprising:
a connection conduit having a sample holder connection end for connecting said sample holder, said connection conduit having a filtrate holder connection end for connecting said filtrate holder, to connect said sample holder to said filtrate holder; and a filter mounted in said connection conduit, whereby the suspension sample must pass through said filter when passing from said sample holder to said filtrate holder.
25. The connection apparatus as claimed in claim 24, further comprising a diaphragm rupturing mechanism, wherein said diaphragm rupturing mechanism is a male shaped protrusion.
26. The connection apparatus as claimed in claim 25, further comprising a support member, wherein said male shaped protrusion is connected to said support member.
27. The connection apparatus as claimed in claim 26, whereiin said support member is moveably mounted on said connection conduit.
28. The connection apparatus as claimed in claim 27, wherein said support member is moveable from a first position to a second position.
29. The connection apparatus as claimed in claim 28, further comprising an inlet in said connection conduit, wherein said inlet defines an opening in said conduit through which a solution may be passed.
30. The connection apparatus as claimed in claim 29, wherein said inlet is resealable.
31. The connection apparatus as claimed in claim 30, wherein said filter is positioned in said connection conduit.
32. The connection apparatus as claimed in claim 31, wherein said diaphragm rupturing mechanism is positioned in said connection conduit.
33. The connection apparatus as claimed in claim 32, wherein movement of said support member to said second position blocks said inlet, and said support member does not block said inlet when said support member is in said first position.
34. A method of filtering a liquid sample for analysis using a sample holder having an entry end portion and an exit end portion, said exit end portion rupturably sealed, a connection conduit having a sample holder connection end portion and a filtrate holder connection end portion, and a filtrate holder having a receiving end portion, comprising the steps of:
collecting the sample through said entry end portion of said sample holder;
attaching said exit end portion of said sample holder to said sample holder connection end portion of said connection conduit;
attaching said receiving end portion of said filtrate holder to said filtrate holder connection end portion of said connection conduit;
rupturing a rupturable seal of said exit end portion of said sample holder, on a male shaped protrusion mounted within said connection conduit;
allowing the sample to flow from said exit end portion of said sample holder through a filter located within said connection conduit;
allowing the filtered sample to exit said connection conduit;

allowing the filtered sample to enter said receiving end portion of said filtrate holder; and removing said filtrate holder from said connection conduit.
35. An apparatus for collection of a suspension sample for filtration comprising:
a sample holder for collecting a sample, wherein said sample holder has a entry end portion and an exit end portion, said entry end portion of raid sample holder being closeable and said exit end portion of said sample holder having a diaphragm normally closing said exit end portion and being rupturable to release said sample;
a filtrate holder for receiving said sample, wherein said filtrates holder has a receiving end portion for receiving said sample, and wherein said filtrate holder is closeable at said receiving end portion;
a connection conduit, wherein said connection conduit has a sample holder connection end for connection to said sample holder and a filtrate holder connection end for connection to said filtrate holder, thereby connecting said sample holder to said filtrate holder;
a support member mounted in said connection conduit;
a rupturing device connected to a support member, said rupturing device for rupturing said diaphragm of said exit end portion of said sample holder, wherein said rupturing device for rupturing said diaphragm is a male shaped protrusion, said male shaped protrusion defining a pathway through which the suspension sample exits said sample holder; and a filter mounted in said connection conduit, whereby the sample must pass through said filter before entering said filtrate holder.
36. The apparatus as claimed in claim 35, wherein said connection conduit has an inlet, said inlet defining an opening though which a solution may pass.
37. The apparatus as claimed in claim 36, wherein said support member is moveably mounted in said connection conduit.
38. The apparatus as claimed in claim 35, wherein said exit end portion of said sample holder is closeable whereby said diaphragm is protected from rupture.
39. The apparatus as claimed in claim 36, wherein said inlet is resealable.
40. The apparatus as claimed in claim 39, wherein said sample holder and said filtrate holder have graduated markings inscribed thereon for direct measurement of sample volume.
41. A filtering system kit comprising:
at least one sample holder for collecting a sample, wherein said sample holder has an entry end portion and an exit end portion, said entry end portion of said sample holder being closeable and said exit end portion of said sample holder having a diaphragm normally closing said exit end portion and being rupturable to release said sample;
at least one filtrate holder for receiving said sample, wherein said filtrate holder has a receiving end portion for receiving said sample, and wherein said filtrate holder is closeable at said receiving end portion;
at least one connection conduit, wherein said connection conduit has a sample holder connection end for connection to said sample holder and a filtrate holder connection end for connection to said filtrate holder, thereby connecting said sample holder to said filtrate holder;
a rupturing device in said connection conduit, sand rupturing device for rupturing said diaphragm of said exit end portion of said sample holder, wherein said rupturing device for rupturing said diaphragm is a male shaped protrusion, said male shaped protrusion defining a pathway through which said sample exits said sample holder;
at least one filter mountable in said connection conduit, whereby said sample must pass through said filter before entering said filtrate holder.
at least one closure for closing said at least one sample holder and filtrate holder; and at least one reagent for use in said filtering system.
CA2433235A 2002-07-03 2003-06-25 Apparatus and method for filtering biological samples Expired - Fee Related CA2433235C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/188,873 US7176034B2 (en) 2002-07-03 2002-07-03 Apparatus and method for filtering biological samples
US10/188,873 2002-07-03

Publications (2)

Publication Number Publication Date
CA2433235A1 CA2433235A1 (en) 2004-01-03
CA2433235C true CA2433235C (en) 2010-08-17

Family

ID=29999561

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2433235A Expired - Fee Related CA2433235C (en) 2002-07-03 2003-06-25 Apparatus and method for filtering biological samples

Country Status (11)

Country Link
US (1) US7176034B2 (en)
EP (1) EP1517735B1 (en)
JP (1) JP4423351B2 (en)
AT (1) ATE456969T1 (en)
AU (1) AU2003245776B2 (en)
CA (1) CA2433235C (en)
DE (1) DE60331188D1 (en)
ES (1) ES2340477T3 (en)
NZ (1) NZ537438A (en)
WO (1) WO2004004865A1 (en)
ZA (1) ZA200410405B (en)

Families Citing this family (84)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6951762B2 (en) * 1998-12-23 2005-10-04 Zuk Jr Peter Apparatus comprising a disposable device and reusable instrument for synthesizing chemical compounds, and for testing chemical compounds for solubility
US7832566B2 (en) 2002-05-24 2010-11-16 Biomet Biologics, Llc Method and apparatus for separating and concentrating a component from a multi-component material including macroparticles
US20030205538A1 (en) 2002-05-03 2003-11-06 Randel Dorian Methods and apparatus for isolating platelets from blood
US7845499B2 (en) 2002-05-24 2010-12-07 Biomet Biologics, Llc Apparatus and method for separating and concentrating fluids containing multiple components
US20060278588A1 (en) 2002-05-24 2006-12-14 Woodell-May Jennifer E Apparatus and method for separating and concentrating fluids containing multiple components
US20060111620A1 (en) * 2004-11-23 2006-05-25 Squilla John R Providing medical services at a kiosk
TWI247084B (en) * 2004-12-17 2006-01-11 Ind Tech Res Inst Filter column module
GB2422794B (en) * 2005-02-05 2009-04-22 Diasys Europ Ltd Filtration device for biological samples
US7854343B2 (en) * 2005-03-10 2010-12-21 Labcyte Inc. Fluid containers with reservoirs in their closures and methods of use
TW200643396A (en) * 2005-03-14 2006-12-16 Nipro Corp Specimen material collection liquid container
AU2006256359A1 (en) * 2005-06-08 2006-12-14 Nipro Corporation Specimen sampling liquid container
WO2007047628A2 (en) * 2005-10-17 2007-04-26 Advanced Technology Materials, Inc. Interconnect and method for joining receptacles
GB2433219A (en) * 2005-12-16 2007-06-20 Diasys Europ Ltd A filtering device
JP5074485B2 (en) * 2006-05-22 2012-11-14 スリーエム イノベイティブ プロパティズ カンパニー System and method for preparing a sample
US8567609B2 (en) 2006-05-25 2013-10-29 Biomet Biologics, Llc Apparatus and method for separating and concentrating fluids containing multiple components
JP4801030B2 (en) * 2006-09-27 2011-10-26 株式会社シン・コーポレイション Inspection kit
US7964098B2 (en) * 2007-02-06 2011-06-21 Alpha-Tec Systems, Inc. Apparatus and method for filtering biological samples
BRMU8700248Y1 (en) * 2007-02-12 2016-12-20 José Carlos Lapenna constructive arrangement applied to a sample collector for parasitological examination of feces
US8328024B2 (en) 2007-04-12 2012-12-11 Hanuman, Llc Buoy suspension fractionation system
JP5479319B2 (en) 2007-04-12 2014-04-23 バイオメット・バイオロジックス・リミテッド・ライアビリティ・カンパニー Buoy suspension fractionation system
WO2009074177A1 (en) * 2007-12-12 2009-06-18 Tecan Trading Ag Forensic sample processor
EP2567692B1 (en) 2008-02-27 2016-04-06 Biomet Biologics, LLC Use of a device for obtaining interleukin-1 receptor antagonist rich solutions
US8337711B2 (en) 2008-02-29 2012-12-25 Biomet Biologics, Llc System and process for separating a material
WO2010093840A1 (en) * 2009-02-11 2010-08-19 M Biosciences, Inc. Devices and methods for overlaying blood or cellular suspensions
US8187475B2 (en) 2009-03-06 2012-05-29 Biomet Biologics, Llc Method and apparatus for producing autologous thrombin
US8313954B2 (en) 2009-04-03 2012-11-20 Biomet Biologics, Llc All-in-one means of separating blood components
US9011800B2 (en) * 2009-07-16 2015-04-21 Biomet Biologics, Llc Method and apparatus for separating biological materials
CN102782472B (en) * 2009-10-02 2016-04-06 生命科技公司 Sample preparation apparatus and method
EP2485842A4 (en) * 2009-10-05 2018-01-10 Battelle Memorial Institute Sample preparation disposable devices and sample collection and preparation methods using same
US8585905B2 (en) * 2010-02-26 2013-11-19 Waters Technologies Corporation Devices, kits and methods for performing chemical processing
US8591391B2 (en) 2010-04-12 2013-11-26 Biomet Biologics, Llc Method and apparatus for separating a material
DE102010023229A1 (en) * 2010-06-09 2011-12-15 Otto Berners Device and method for transporting and processing cellular material
WO2014039697A1 (en) 2012-09-06 2014-03-13 The Gid Group, Inc. Tissue processing apparatus and method for processing adipose tissue
EP2593768A1 (en) * 2010-07-14 2013-05-22 Qiagen GmbH New storage, collection or isolation device
CN107202727B (en) * 2010-07-14 2020-12-22 恰根有限公司 Device for separating and/or purifying biomolecules
ES2557562T3 (en) * 2011-02-07 2016-01-27 1Cryobio Ag Frangible sample storage container
EP2688675A1 (en) * 2011-03-24 2014-01-29 Boehringer Ingelheim Microparts GmbH Device and method for removing a supernatant of a liquid sample, and use of a valve device having a soluble membrane
WO2013042435A1 (en) * 2011-09-20 2013-03-28 富士紡ホールディングス株式会社 Reagent container
CN102539214A (en) * 2012-02-23 2012-07-04 杭州遂真生物技术有限公司 Sample-processing combined test tube and sample-processing method
CN102564796A (en) * 2012-02-24 2012-07-11 万华普曼生物工程有限公司 Excrement collector
US8322539B1 (en) * 2012-03-02 2012-12-04 Scientific Plastic Products, Inc. Filter vial
ES2841112T3 (en) * 2012-05-30 2021-07-07 Lifecell Corp Device for collecting, processing and transferring adipose tissue
US20130345596A1 (en) * 2012-06-25 2013-12-26 David S. Zimmon Apparatus and methods for removing and collecting biopsy specimens from biopsy devices with fixation and preparation for histopathological processing or other analysis
US11090338B2 (en) 2012-07-13 2021-08-17 Lifecell Corporation Methods for improved treatment of adipose tissue
US9642956B2 (en) 2012-08-27 2017-05-09 Biomet Biologics, Llc Apparatus and method for separating and concentrating fluids containing multiple components
JP2014098661A (en) * 2012-11-15 2014-05-29 Olympus Corp Sample manufacturing device
WO2014081877A1 (en) 2012-11-20 2014-05-30 The Trustees Of Columbia University In The City Of New York Medical apparatus and method for collecting biological samples
US9895418B2 (en) 2013-03-15 2018-02-20 Biomet Biologics, Llc Treatment of peripheral vascular disease using protein solutions
US9950035B2 (en) 2013-03-15 2018-04-24 Biomet Biologics, Llc Methods and non-immunogenic compositions for treating inflammatory disorders
US20140271589A1 (en) 2013-03-15 2014-09-18 Biomet Biologics, Llc Treatment of collagen defects using protein solutions
US10143725B2 (en) 2013-03-15 2018-12-04 Biomet Biologics, Llc Treatment of pain using protein solutions
US10208095B2 (en) 2013-03-15 2019-02-19 Biomet Manufacturing, Llc Methods for making cytokine compositions from tissues using non-centrifugal methods
US10035146B2 (en) * 2013-05-24 2018-07-31 Premier Biotech, Inc. Multi-stage oral-fluid testing device
AU2014278039A1 (en) * 2013-06-12 2016-01-07 Oasis Diagnostics Corporation RNA/protein/DNA preferential fluid sample collection system and methods
US10302535B2 (en) * 2013-09-12 2019-05-28 CellectGen, Inc. Biofluid collection and filtration device
EP3069118A4 (en) * 2013-11-14 2017-07-05 Abon Biopharm (Hangzhou) Co., Ltd. A device and method for using the device
EP3137139A1 (en) 2014-05-02 2017-03-08 Lifecell Corporation Injection sensor with feedback mechanism
EP3190978B1 (en) * 2014-09-12 2021-10-27 Vib Vzw Biological sampling and storage container
US20160114317A1 (en) * 2014-10-27 2016-04-28 Moishe Bodner Device and system for sampling and analyzing a liquid specimen
EP3258979A1 (en) 2015-02-19 2017-12-27 Lifecell Corporation Tissue processing device and associated systems and methods
FI20155107A (en) * 2015-02-19 2016-08-20 Thermo Fisher Scientific Oy The sample container
GB2535998A (en) * 2015-02-27 2016-09-07 Intelligent Fingerprinting Ltd A device for receiving and analysing a sample
US10421069B2 (en) * 2015-07-06 2019-09-24 Hans-Werner Heinrich Multifunctional system for particle separation
EP3633027A1 (en) 2015-10-21 2020-04-08 LifeCell Corporation Methods for tube management
CA3002538A1 (en) 2015-10-21 2017-04-27 Lifecell Corporation Systems and methods for medical device control
EP3847965A1 (en) * 2015-12-11 2021-07-14 Babson Diagnostics, Inc. Specimen container and method for separating serum or plasma from whole blood
EP3393390A1 (en) 2015-12-22 2018-10-31 LifeCell Corporation Syringe filling device for fat transfer
AU2017319140B2 (en) 2016-08-30 2022-11-17 Lifecell Corporation Systems and methods for medical device control
CZ2016820A3 (en) * 2016-12-22 2018-09-05 Univerzita Palackého v Olomouci A device for facilitating the handling of solvents, mixtures and samples on carriers, a kit for handling them, and the use of the device and/or the kit
GB201703383D0 (en) 2017-03-02 2017-04-19 Gargle Tech Ltd Testing for particulates
WO2020049569A2 (en) 2018-09-05 2020-03-12 Hero Scientific Ltd. Testing for particulates
US10974248B2 (en) * 2018-03-14 2021-04-13 MTC Bio Inc. Adapter for laboratory cell strainer
DE102019121342B4 (en) * 2018-08-15 2021-03-18 Mann+Hummel Gmbh Filter element for use as a particle filter in a cooling circuit of an electrochemical energy converter and use of the filter element in an arrangement with an electrochemical energy converter and a cooling circuit
KR102522436B1 (en) * 2019-06-25 2023-04-17 (주)메디젠휴먼케어 Liquid Biopsy Collecting Apparatus
IT201900016112A1 (en) * 2019-09-11 2021-03-11 Copan Italia Spa RELEASE CAP, CONTAINER WITH CAP AND ASSOCIATED RELEASE METHOD
EP4027894A4 (en) * 2019-10-31 2023-09-13 Orasecure LLC Oral fluid, saliva & sputum collection device and method
US20210196246A1 (en) * 2019-12-30 2021-07-01 Northeastern University Suction Based Saliva Treatment and Collector Device
KR200496916Y1 (en) * 2020-10-19 2023-06-01 임동선 Saliva Collection Kit
EP4165385B1 (en) 2021-01-06 2024-04-17 Hero Scientific Ltd Filtration sampling devices
IT202100001025A1 (en) * 2021-01-21 2022-07-21 Comedical S R L SALIVA SAMPLE COLLECTION DEVICE
US20220401959A1 (en) * 2021-06-17 2022-12-22 Becton, Dickinson And Company Dried Dye Reagent Devices and Methods for Making and Using The Same
NL2032490B1 (en) * 2022-07-14 2024-01-25 Daklapack Europe B V Specimen collection device for home sampling and transport.
WO2024013313A1 (en) 2022-07-14 2024-01-18 Daklapack Europe B.V. Specimen collection device for home sampling and transport
CN117288553B (en) * 2023-11-24 2024-01-26 烟台至公生物医药科技有限公司 Sample rapid filtering type purifying device for portable mass spectrometer

Family Cites Families (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2110237A (en) * 1936-03-06 1938-03-08 Swift & Co Sediment tester
US2584397A (en) * 1945-10-03 1952-02-05 Louis K Pitman Apparatus for transferring liquid from one container to another
US2775350A (en) * 1953-12-28 1956-12-25 Hugh A Jones Filters
US3300051A (en) * 1963-09-26 1967-01-24 Internat Equipment Co Filter tube for use in a centrifuge
US3539300A (en) * 1967-10-23 1970-11-10 Schering Corp Body fluid collector and separator having improved flow rate
US3615222A (en) 1968-09-04 1971-10-26 New England Nuclear Corp Method and apparatus for measuring the amount of a component in a biological fluid
US3583627A (en) * 1969-09-04 1971-06-08 Onslow H Wilson Apparatus for the concentration of macromolecules and subcellular particles from dilute solutions
US3682321A (en) * 1970-03-02 1972-08-08 Volu Sol Chem And Mfg Co Inc Throw-away filter assembly
US3661265A (en) 1970-07-27 1972-05-09 Contemporary Research And Dev Serum separator type container
US3701434A (en) 1971-03-15 1972-10-31 Hugh C Moore Test tube system for separating blood into serum and red cells
US3788483A (en) 1972-05-26 1974-01-29 W Conway Apparatus for filtration
US3955423A (en) 1972-09-18 1976-05-11 Marvin Padover Liquid sampling method
US3932277A (en) 1974-03-29 1976-01-13 Bio-Logics Products, Inc. Method and apparatus for separating blood fractions
US3977555A (en) * 1974-05-07 1976-08-31 Pharmaco, Inc. Protective safety cap for medicament vial
US3939822A (en) 1974-08-14 1976-02-24 Jack Markowitz Disposable blood collection and filtering device
US3969250A (en) 1975-03-10 1976-07-13 Farr Andrew F Apparatus for preparing liquid samples for analysis in automatic analyzers
US4035294A (en) 1975-05-23 1977-07-12 Denver Chemical Manufacturing Company Pressure differential filtering device and method
US4131549A (en) 1977-05-16 1978-12-26 Ferrara Louis T Serum separation device
US4146153A (en) * 1977-07-07 1979-03-27 Knight Development Corporation Sterile dispensing device
US4353868A (en) * 1981-05-29 1982-10-12 Sherwood Medical Industries Inc. Specimen collecting device
US4464254A (en) 1982-06-03 1984-08-07 Porex Technologies, Corp. Device for separating serum from blood sample
JPS6077768A (en) 1983-10-06 1985-05-02 テルモ株式会社 Liquid dialytic apparatus
US4678559A (en) 1984-07-23 1987-07-07 Andreas Szabados Test specimen container for pasty specimen material
DE3722562A1 (en) 1987-07-08 1989-01-19 Andreas Szabados Annular filter body
US4783318A (en) * 1987-10-02 1988-11-08 The State Of Minnesota Apparatus for environmental leaching testing
US4891134A (en) 1988-01-25 1990-01-02 Abbott Laboratories Sample filtration device
JPH0755498Y2 (en) * 1988-07-13 1995-12-20 川澄化学工業株式会社 Blood collection device for blood transfusion and blood test
US5429803A (en) * 1991-04-18 1995-07-04 Lamina, Inc. Liquid specimen container and attachable testing modules
JP2523938B2 (en) * 1989-09-18 1996-08-14 テルモ株式会社 Platelet purification filter
JPH0774772B2 (en) * 1990-12-31 1995-08-09 エイ． レビン ロバート Blood sampling assembly, target cell collection method and target component collection method
US5322800A (en) 1991-06-26 1994-06-21 The United States Of America As Represented By The Secretary Of The Interior Method and device for safely preserving aqueous field samples using acid or base
US5501841A (en) 1991-11-14 1996-03-26 Artchem, Inc. Connection-type treatment system for micro solution and method of treatment
JPH07110329A (en) * 1993-08-20 1995-04-25 Dainippon Printing Co Ltd Detector for occult blood in feces
US5976824A (en) * 1993-11-24 1999-11-02 Abbott Laboratories Method and apparatus for collecting a cell sample from a liquid specimen
US5582696A (en) * 1994-05-31 1996-12-10 Health Craft International, Inc. Glucose level monitoring apparatus
US5603900A (en) * 1995-05-19 1997-02-18 Millipore Investment Holdings Limited Vacuum filter device
US5786227A (en) 1995-06-07 1998-07-28 Biex, Inc. Fluid collection kit and method
US5786228A (en) 1995-06-07 1998-07-28 Biex, Inc. Fluid collection kit and method
US5556544A (en) 1995-09-08 1996-09-17 Didier; Emmanuel R. Concentrator & filter
US5882943A (en) 1996-07-31 1999-03-16 Aldeen; William Erick Filtration apparatus, kit and method for processing parasite samples
US6221655B1 (en) 1998-08-01 2001-04-24 Cytosignal Spin filter assembly for isolation and analysis
EP1224258A2 (en) * 1999-10-29 2002-07-24 Pall Corporation Biological fluid processing
DE10020826A1 (en) 2000-04-28 2001-10-31 Gert Schlueter Method and device for filtering a liquid sample, in particular for obtaining medical preparations

Also Published As

Publication number Publication date
NZ537438A (en) 2008-02-29
AU2003245776A1 (en) 2004-01-23
US7176034B2 (en) 2007-02-13
AU2003245776B2 (en) 2009-03-05
CA2433235A1 (en) 2004-01-03
EP1517735B1 (en) 2010-02-03
JP2005531774A (en) 2005-10-20
EP1517735A1 (en) 2005-03-30
ES2340477T3 (en) 2010-06-04
US20040005246A1 (en) 2004-01-08
DE60331188D1 (en) 2010-03-25
ZA200410405B (en) 2005-12-28
ATE456969T1 (en) 2010-02-15
JP4423351B2 (en) 2010-03-03
WO2004004865A1 (en) 2004-01-15

Similar Documents

Publication Publication Date Title
CA2433235C (en) Apparatus and method for filtering biological samples
JP3658601B2 (en) Disposable test bottle with sample transfer device for discharging the sample into the reagent
EP2923191B1 (en) Medical apparatus and method for collecting biological samples
EP0378353B1 (en) Apparatus for collecting biological fluid
US5353806A (en) Liquid collection device
KR20010072638A (en) Filtration and extraction device and method of using the same
US6074183A (en) Peristaltic system and method for plasma separation and blood dispensation
EP1346773A2 (en) Apparatus and method for separating a biological sample
EP1230028A1 (en) Method and apparatus for directly sampling a fluid for microfiltration
US20060060531A1 (en) Method and apparatus for directly sampling a fluid for microfiltration
AU8666798A (en) Analytical cartridge
EP3288459B1 (en) Specimen collection and delivery apparatus
US6063038A (en) Devices and methods for collecting fecal antigen specimens
US5275731A (en) Apparatus for rapidly separating blood into filtered fractions
US20040025603A1 (en) Test tube insert
US20040025935A1 (en) Test tube insert
CN212807652U (en) Fecal sample collection device with one-way valve
EP0508010A1 (en) Biological fluids testing membrane module and method of conducting same
WO2008012847A1 (en) Device and method for the preparation of biological samples for cytological and histological analyses
US20210394173A1 (en) Biological specimen separation instrument
US20220071532A1 (en) Biological fluid sampling device
US20240082109A1 (en) Sampling Device
JP3597827B6 (en) Biological sample separation instrument and separation method thereof
JP2023535553A (en) Fluid sample collection vessel with removable tube retainer
CN115856337A (en) Devices, systems, and methods for sample capture and extraction

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed

Effective date: 20150625