CA2461885A1 - Biochemical assay detection in a liquid receptacle using a fiber optic exciter - Google Patents

Biochemical assay detection in a liquid receptacle using a fiber optic exciter Download PDF

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Publication number
CA2461885A1
CA2461885A1 CA002461885A CA2461885A CA2461885A1 CA 2461885 A1 CA2461885 A1 CA 2461885A1 CA 002461885 A CA002461885 A CA 002461885A CA 2461885 A CA2461885 A CA 2461885A CA 2461885 A1 CA2461885 A1 CA 2461885A1
Authority
CA
Canada
Prior art keywords
light
receptacle
optical fiber
open mouth
collimating lens
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002461885A
Other languages
French (fr)
Other versions
CA2461885C (en
Inventor
James W. Hillendahl
David E. Waldbeser
Harry R. Naghieh
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bio Rad Laboratories Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2461885A1 publication Critical patent/CA2461885A1/en
Application granted granted Critical
Publication of CA2461885C publication Critical patent/CA2461885C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/251—Colorimeters; Construction thereof
    • G01N21/253—Colorimeters; Construction thereof for batch operation, i.e. multisample apparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G01N2021/6471—Special filters, filter wheel
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6484—Optical fibres

Abstract

Biochemical assays that are performed in multi-well plates (11) and that utilize a system that supplies excitation light through an optical fiber (21 , 22) that transmits light from an excitation light source (14) to the well (12). Emission light produced by the excitation is then collected by a collimating lens (31) and converted to a signal that is compiled for analysi s. The optical fiber (21, 22) and collimating lens (31) can either be on the sa me side of the receptacle or on opposite sides, i.e., one above the other below . When the optical fiber and the collimating lens are both on the open side of the receptacle, they are arranged such that the direction of travel of the excitation light and the direction along which the emission light is collect ed are not coaxial, and preferably both are at an acute angle to the axis norma l to the mouth of the receptacle.

Claims (11)

1. Apparatus for generating and detecting an optical signal from the contents of a liquid receptacle resulting from a bioassay performed therein, said apparatus comprising:
an excitation light source, an optical fiber arranged to transmit excitation light from said excitation light source to said receptacle with a divergent beam, and a collimating lens arranged to collect emission light from said receptacle;
and detection means for receiving emission light collected by said collimating lens and for generating a signal representative of said emission light thus collected.
2. Apparatus in accordance with claim 1 in which said receptacle has an open mouth, said optical fiber is arranged to direct said excitation light through said open mouth, and said collimating lens is arranged to collect said emission light through said open mouth along an emission collection path that is not coaxial with said optical fiber.
3. Apparatus in accordance with claim 2 in which said open mouth defines an axis normal thereto, and said optical fiber and said emission collection path form an angle of from about 5° to about 25° relative to said axis.
4. Apparatus in accordance with claim 1 in which said receptacle has a light-transmissive floor and an open mouth opposite said light-transmissive floor, said optical fiber is arranged to transmit light to the contents of said receptacle through one of said light-transmissive floor and said open mouth, and said collimating lens is arranged to collect said emission light through the other of said light-transmissive floor and said open mouth.
5. Apparatus in accordance with claim 1 in which said excitation light source is a light-emitting diode and said optical fiber is secured to, and in direct contact with, said diode, either by direct coupling or through a member selected from the group consisting of an optically clear adhesive, an optically clear gel, an optically clear oil, and a lens system directing light from said diode into said optical fiber.
6. Apparatus in accordance with claim 1 in which said liquid receptacle is a well of a multi-well plate, and said apparatus further comprises means for rastering either said multi-well plate relative to said optical fiber and said collimating lens or said optical fiber and said collimating lens relative to said multi-well plate until light from said excitation light source has illuminated, and emission light has been collected from, all wells of said multi-well plate.
7. A method for generating and detecting an optical signal from the contents of a liquid receptacle resulting from a bioassay performed in said receptacle, said method comprising:
(a) illuminating the contents of said receptacle with light from an excitation light source through an optical fiber directing a divergent beam at said receptacle; and (b) collecting emission light from said receptacle by a collimating lens and directing emission light thus collected to a detector.
8. A method in accordance with claim 7 in which said receptacle has an open mouth and said method comprises illuminating the contents of said receptacle through said open mouth and collecting said emission light from said receptacle through said open mouth.
9. A method in accordance with claim 7 in which said receptacle has a light-transmissive floor and an open mouth opposite said light-transmissive floor, step (a) comprises directing said divergent beam through one of said light-transmissive floor and said open mouth, and step (b) comprises collecting said emission light through the other of said light-transmissive floor and said open mouth.
10. A method for generating and detecting optical signals from a plurality of biochemical assays performed in individual wells of a multi-well plate, said method comprising:
(a) illuminating the contents of a single well with light from an excitation light source through an optical fiber directing a divergent beam at said well;
(b) collecting emission light from said well by a collimating lens and directing emission light thus collected to a detector; and (c) rastering either said multi-well plate relative to said optical fiber and said collimating lens or said optical and said collimating lens relative to said multi-well plate until light from said excitation light source has illuminated, and emission light has been collected from, all wells of said multi-well plate.
11
CA2461885A 2001-09-27 2002-09-24 Biochemical assay detection in a liquid receptacle using a fiber optic exciter Expired - Fee Related CA2461885C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US32585501P 2001-09-27 2001-09-27
US32587601P 2001-09-27 2001-09-27
US60/325,855 2001-09-27
US60/325,876 2001-09-27
PCT/US2002/030301 WO2003027741A2 (en) 2001-09-27 2002-09-24 Biochemical assay detection in a liquid receptacle using a fiber optic exciter

Publications (2)

Publication Number Publication Date
CA2461885A1 true CA2461885A1 (en) 2003-04-03
CA2461885C CA2461885C (en) 2011-11-29

Family

ID=26985146

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2461885A Expired - Fee Related CA2461885C (en) 2001-09-27 2002-09-24 Biochemical assay detection in a liquid receptacle using a fiber optic exciter

Country Status (5)

Country Link
US (2) US7218810B2 (en)
EP (1) EP1430341A4 (en)
JP (1) JP4464130B2 (en)
CA (1) CA2461885C (en)
WO (1) WO2003027741A2 (en)

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Also Published As

Publication number Publication date
WO2003027741A2 (en) 2003-04-03
US7310462B2 (en) 2007-12-18
US20070263954A1 (en) 2007-11-15
JP2005529308A (en) 2005-09-29
JP4464130B2 (en) 2010-05-19
WO2003027741A3 (en) 2003-07-10
US20030063851A1 (en) 2003-04-03
CA2461885C (en) 2011-11-29
EP1430341A2 (en) 2004-06-23
EP1430341A4 (en) 2006-01-18
US7218810B2 (en) 2007-05-15

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Effective date: 20130924