CA2466499C - Use of a mixture of sodium hyaluronate and chondroitin sulfate for the treatment of osteoarthritis - Google Patents
Use of a mixture of sodium hyaluronate and chondroitin sulfate for the treatment of osteoarthritis Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- A61K31/726—Glycosaminoglycans, i.e. mucopolysaccharides
- A61K31/728—Hyaluronic acid
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- A61K31/737—Sulfated polysaccharides, e.g. chondroitin sulfate, dermatan sulfate
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
- A61K9/0024—Solid, semi-solid or solidifying implants, which are implanted or injected in body tissue
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/26—Mixtures of macromolecular compounds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
- A61P19/02—Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P41/00—Drugs used in surgical methods, e.g. surgery adjuvants for preventing adhesion or for vitreum substitution
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/24—Materials or treatment for tissue regeneration for joint reconstruction
Abstract
This invention is related to the use of the composition formed by sodium hyaluronate and sodium chondroitin sulfate for the treatment of chondral lesions in osteoarthritis, and to the use of such composition in the manufacture of a product for such treatment.
Description
USE OF A MIXTURE OF SODIUM HYALURONATE AND CHONDROITIN SULFATE FOR THE
TREATMENT OF OSTEOARTHRITIS
FIELD OF THE INVENTION
This invention is related to methods and compositions for the treatment of arthritis, and in particular the treatment of osteochondral lesions associated with osteoarthritis by means of the intraarticular application of a mixture of hyaluronate and chondroitin sulfate.
Specifically, this discovery has its preferred application in the lubrication and regeneration of the articular cartilage damaged by grade I and grade II osteoarthritis of human or animal (preferably mammalian) joints including without limitation the knee, shoulder, sacroiliac, hip, ankle, elbow, interphalangeal and wrist joints through the intraarticular, application of a mixture of sodium hyaluronate and dhondroitin sulfate in gel.
The main objectives of the invention are to introduce the new medical use of the mixture of sodium hyaluronate and sodium chondroitin sulfate for the regeneration of articular cartilage damaged by osteoarthritis, corresponding treatment regimens, and the use of the components in the manufacture of products for such therapies.
BACKGROUND OF THE INVENTION
Osteoarthritis is a condition that affects many millions of persons throughout the world. Previously, there has been no effective treatment that specifically targets the chondral
TREATMENT OF OSTEOARTHRITIS
FIELD OF THE INVENTION
This invention is related to methods and compositions for the treatment of arthritis, and in particular the treatment of osteochondral lesions associated with osteoarthritis by means of the intraarticular application of a mixture of hyaluronate and chondroitin sulfate.
Specifically, this discovery has its preferred application in the lubrication and regeneration of the articular cartilage damaged by grade I and grade II osteoarthritis of human or animal (preferably mammalian) joints including without limitation the knee, shoulder, sacroiliac, hip, ankle, elbow, interphalangeal and wrist joints through the intraarticular, application of a mixture of sodium hyaluronate and dhondroitin sulfate in gel.
The main objectives of the invention are to introduce the new medical use of the mixture of sodium hyaluronate and sodium chondroitin sulfate for the regeneration of articular cartilage damaged by osteoarthritis, corresponding treatment regimens, and the use of the components in the manufacture of products for such therapies.
BACKGROUND OF THE INVENTION
Osteoarthritis is a condition that affects many millions of persons throughout the world. Previously, there has been no effective treatment that specifically targets the chondral
2 lesion associated with the condition and promotes the in situ regeneration of cartilage at that site.
This disease consists of the gradual degeneration and destruction of the articular cartilage due to traumas, structural deformities of the joints, and overweight. This process thins the cartilage through a phenomenon called apoptosis, or programmed cell death. When the surface area has disappeared due to the thinning, it is considered grade I
osteoarthritis; when the tangential surface area .has also disappeared, it is characterized as grade II osteoarthritis There are other levels of degeneration and destruction, which affect the deep and the calcified layers that border with the subchondral bone.
The clinical manifestations of the development of the condition are: swelling of the joint, pain, crepitation and functional disability that, gradually and steadily, hinders physical mobility, e.g. the taking of lengthy walks and, depending on the affected joint, forced flexion and extension movements. As the condition worsens, pain begins to. limit even minimum efforts and can persist at rest making it difficult to sleep. If the condition persists without correction and/or therapy, the joint is totally destroyed, leading the patient to major replacement surgery with total prosthesis, or to disability.
Therapeutic methods for the correction of the articular cartilage lesions that appear during the osteoarthritic disease have been developed, but so far none of them have been able to achieve the regeneration of articular cartilage in situ and in vivo.
The prior art methods include the following:
This disease consists of the gradual degeneration and destruction of the articular cartilage due to traumas, structural deformities of the joints, and overweight. This process thins the cartilage through a phenomenon called apoptosis, or programmed cell death. When the surface area has disappeared due to the thinning, it is considered grade I
osteoarthritis; when the tangential surface area .has also disappeared, it is characterized as grade II osteoarthritis There are other levels of degeneration and destruction, which affect the deep and the calcified layers that border with the subchondral bone.
The clinical manifestations of the development of the condition are: swelling of the joint, pain, crepitation and functional disability that, gradually and steadily, hinders physical mobility, e.g. the taking of lengthy walks and, depending on the affected joint, forced flexion and extension movements. As the condition worsens, pain begins to. limit even minimum efforts and can persist at rest making it difficult to sleep. If the condition persists without correction and/or therapy, the joint is totally destroyed, leading the patient to major replacement surgery with total prosthesis, or to disability.
Therapeutic methods for the correction of the articular cartilage lesions that appear during the osteoarthritic disease have been developed, but so far none of them have been able to achieve the regeneration of articular cartilage in situ and in vivo.
The prior art methods include the following:
3 a) The application of tendinous, periosteal, fascial, muscular or perichondral grafts.
c b) The implantation of fibrin or cultured chondrocytes.
c) The administration of chondrogenic stimulating factors such as "insulin-like growth factors I and TGF-B":
d) Implantation of synthetic matrices, such as collagen and carbon fiber.
e) Others, such as electromagnetic fields. (J.' Buckwalter, M.D.; Van C. Now, Ph. D. and Anthony Ratcliffe, Ph.D. Journal of the American Academy of Orthopaedic Surgery 1994; 2:192-202).
All of these have reported minimal and incomplete results with formation of repair, but not regenerative tissue, resulting in a poor quality tissue that can neither support the weighted load nor allow the restoration of an articular function with normal movement.
One treatment that has 74% to 90% effectiveness and produces excellent results, similar to that presented in this invention, is the transplantation of cultured autologous chondrocytes. This method of treatment was first reported in 1987 in Sweden and was introduced in 1995 to the United States of America. It consists of taking chondral cellular material from the patient, sending it to a laboratory where it is seeded
c b) The implantation of fibrin or cultured chondrocytes.
c) The administration of chondrogenic stimulating factors such as "insulin-like growth factors I and TGF-B":
d) Implantation of synthetic matrices, such as collagen and carbon fiber.
e) Others, such as electromagnetic fields. (J.' Buckwalter, M.D.; Van C. Now, Ph. D. and Anthony Ratcliffe, Ph.D. Journal of the American Academy of Orthopaedic Surgery 1994; 2:192-202).
All of these have reported minimal and incomplete results with formation of repair, but not regenerative tissue, resulting in a poor quality tissue that can neither support the weighted load nor allow the restoration of an articular function with normal movement.
One treatment that has 74% to 90% effectiveness and produces excellent results, similar to that presented in this invention, is the transplantation of cultured autologous chondrocytes. This method of treatment was first reported in 1987 in Sweden and was introduced in 1995 to the United States of America. It consists of taking chondral cellular material from the patient, sending it to a laboratory where it is seeded
4 in a proper medium for its proliferation, and then, once enough volume is achieved (a variable period that may last from weeks to months), transporting it in a special container, and finally implanting it in the damaged tissues to cover their defects. This is an expensive procedure that requires the patient to be in the operating room for the removal of the necessary cellular material, and subsequently for the implantation of the proliferated material. Furthermore, a significant waiting period is needed for the implant to be ready.
Other, more conventional treatments include anti inf1ammatories, antirheumatics, systemics, physiotherapy, injection of depot steroids and, recently, viscoprotection has emerged.
Viscoprotection involves the intraarticular application of commercially available sodium hyaluronate viscoelastic TM TM TM
materials such as HYLAN c-F 20, SYNVISC, HYALGAN, ARTZ, etc.
The sodium hyaluronate substance does affect the rheology of the synovial fluid, producing an almost immediate sensation of free movement and a marked reduction of pain. It, has been (, proven that the change of the intraarticular fluids attendant to sodium hyaluronate instillation produces a blockage of the nociceptors of subsynovial and capsular tissues and that, in addition to the mechanical factors of the osteochondral pathology, the fluids influence these receptors with their lubricating properties. Thus the change in viscosity of these fluids acts favorably on the painful osteochondral symptoms when sodium hyaluronate is instilled. However, the effect of conventional hyaluronate is temporary because the material remains within the articular chamber for only about 72 hours before it is absorbed and/or metabolized. The residual effects of this substance act on the synovial receptors causing a pain reduction that lasts several weeks and even months.
However, this isolated effect is counterproductive for the course of the disease and for the viability of the cartilage because, as it masks the symptoms, the joint is used with more intensity and its destruction is accelerated as the original problem is not corrected and the the damaged articular cartilage is not restored. Recent studies with a 5 year follow-up with these substances indicate that clinical improvement is significant and that it represents a remission factor of painful symptoms, but only for short and medium term.
Also, adverse effects, characterized by severe pain, significant synovial effusion, rash and ankle edema, have been reported in at least 7.2% of the treated patients. In no instance has hyaluronate therapy been reported to effect cartilage regeneration and long term success. The need exists, therefore, for an improved approach to cartilage regeneration.
As an antecedent to this invention, in 1982, the applicant began applying sodium hyaluronate (SH) to thoroughbred race horses at Hipodromo de las Americas (Las Americas Race Track), in Mexico City, Mexico. The knees and ankles are the most commonly injured joints in these horses. Veterinarians at racetracks in the USA had already used this procedure, observing the beneficial reaction that this viscoelastic material produced in the injured knees of the horses. The applicant considered its use in humans, and conceived of adding some substance to cause the restoration of the damaged surface of the cartilage.
The applicant hypothesized that chondroitin sulfate (CS), the most important part of the aggrecan proteoglycans which are the basis of chondral support, might have a repaving effect.
In 1996 while visiting Alcon Laboratories in Mexico City, the applicant learned that one of the company's ophthalmic products contained both of the above mentioned substances in a gel suspension (VISCOAT ). The inventor obtained detailed information, including the product monograph for VISCOAT that states that is has no reported side effects in intraocular use;
furthermore, there are ample references from efficacy and safety studies of this product.
It was then that the applicant decided to use it experimentally in patients with -osteoarthritis disorders of all degrees, and subsequently analyze the results.
The present study reveals another alternative in the management of osteochondral lesions of the knee through the intraarticular application of a mixture of sodium hyaluronate and sodium chondroitin sulfate. While bound by no theories,- it may be that the remarkable effectiveness of. this therapy is, attributable to the promotion of chondrogenesis s-synergistically combined with the known benefits of viscoelastic therapy.
Implanting an artificial matrix of chondroitin sulfate and sodium hyaluronate may represent an indispensable repair factor, as in it naturally arising chondrocytes can proliferate and restore the continuity of the tissue, regenerating the destroyed cartilage to its original form.
With this matrix, the symptomatic evolution is significantly favorable and long lasting due to the regeneration of cartilage at the chondral lesions. No side effects have been reported except in a patient who reported pain and slight swelling at the site of application, which subsided spontaneously in 24 hours; he was given acetaminophen as an analgesic.
It must be pointed out that in the most preferred usage the product is administered exactly as it is presented for intraocular use and no change is made in the formulation. A
change in presentation with a larger capacity syringe is now being proposed, as the current ophthalmic presentation has 0.5 c.c. and 0.75 c.c. syringes.
It must also be pointed out that although this is the same preparation as that used intraocularly, its use for this purpose is totally different as it is applied in a conventional intraarticular manner as an inductor of chondrogenesis, to regenerate the cartilage destroyed by osteoarthritis.
As previously mentioned, experimental application of this composition in humans started in 1996, and excellent results have been noted. These were confirmed later by arthroscopic studies (direct view of the articular cartilage through the insertion of a camera into the joint), pathological anatomy and histophysiological studies, all of them consistent with the clinical findings that the regeneration of normal articular cartilage was achieved. This is why this treatment is presented as the only currently available procedure that can offer up to 95% regeneration of articular cartilage damaged by grade I and II osteoarthritis in any joint of the human body.
SUMMARY OF THE INVENTION
This invention was developed to solve the problem of the previously available techniques related to the treatment of articular cartilage damaged by osteoarthritis.
This invention introduces a method to achieve regeneration of the articular cartilage by chondrogenic induction through the intraarticular implantation of an artificial matrix in patients and animals with chondromalacia and/or osteoarthritis in any joint, but preferably in human patients with grade I or II osteoarthritis.
The regeneration process is elicited by implanting an artificial matrix formed by a mixture of chondroitin sulfate and hyaluronic acid or pharmaceutically acceptable salts thereof, where naturally arising chondrocytes can settle, and where, as they mature, they form, in groups of 3 or 4, their own definitive hyaline matrix, duplicating the same pattern of the natural cartilage. In this manner the continuity of the articular surface is recovered, mobility is regained, pain is eliminated and thus function is recovered.
The invention also provides a composition adapted to be intraarticularly administered, said composition comprising a mixture of chondroitin sulfate and hyaluronic acid or pharmaceutically acceptable salts thereof for the treatment of degeneration of articular cartilage of a joint.
The invention also provides use of a stable, viscous, buffered aqueous solution which comprises a mixture of chondroitin sulfate at a concentration of about 0.1 to 50% by weight and sodium hyaluronate at a concentration of about 0.1 to 50% by weight in the 8a manufacture of a product for the intraarticular treatment of chondromalacia and/or osteoarthritis.
The invention also provides the use of a mixture of chondroitin sulfate and hyaluronic acid or pharmaceutically acceptable salts thereof for repairing or regenerating cartilage of a mammalian joint characterized by cartilage disease or trauma, said mixture being adapted for intraarticular instillation.
The invention also provides the use of a mixture of chondroitin sulfate and hyaluronic acid or pharmaceutically acceptable salts thereof in the manufacture of a product for intraarticular administration to repair or regenerate cartilage of a mammalian joint characterized by cartilage disease or trauma.
The invention also provides a composition for intraarticular treatment of degeneration of articular cartilage of a joint, the composition comprising a mixture of chondroitin sulfate and hyaluronic acid or pharmaceutically acceptable salts thereof.
The invention also provides a stable, viscous, buffered aqueous solution for the intraarticular treatment of chondromalacia and/or osteoarthritis, the solution comprising a mixture of chondroitin sulfate at a concentration of 0.1 to 50% by weight and sodium hyaluronate at a concentration of 0.1 to 50% by weight in the manufacture of a product.
The invention also provides a mixture for repairing or regenerating cartilage of a mammalian joint characterized by cartilage disease or trauma, the mixture being for intraarticular instillation and the 8b mixture comprising chondroitin sulfate and hyaluronic acid or pharmaceutically acceptable salts thereof.
BRIEF DESCRIPTION OF THE DRAWINGS
Figures 1 to 6 represent pre- and post implantation arthroscopic images from patients who underwent the treatment of the present invention: macro and microscopic confirmation of regeneration of the articular surface with De Novo cartilage with the same characteristics as the original, a conclusive pathology report, and histophysiology tests (S-100 Protein) both of which confirm the characteristics of the regenerated cartilage.
Figures 1 and 2 represent pre- and two years posttreatment arthroscopic images, respectively.
Figure 3 represents the regeneration of the articular cartilage with De Novo cartilage.
Figures 4 and 5 are microscopic images that show the maturing of the zone tangential to the superficial layer where fully developed and maturing chondrocytes are found, surrounded by a hyaline matrix.
Figure 6 is a confirmatory histophysiological image with the application of S-100 protein. It shows the reaction of the cartilaginous tissue to this test with a positive result.
Figures 7 to 11 show .the result of a comparative study of patients treated with NSAIDs and VISCOAT .
CHARACTERISTICS OF THE PRODUCT
In its preferred embodiment, the chondroitin sulfate/hyaluronate composition of the present invention is presented under the trade name of VISCOAT (Alcon Laboratories, Inc., Fort Worth, Texas, USA). It is suitable for the treatment of chondral lesions in all osteoarthritis(OA) in patients, and particularly in those who fail to respond adequately to traditional non-drug therapies and plain analgesics.
The VISCOAT product packaging identifies U.S. Patent Nos.
4,486,416 and 6,051,560. The VISCOAT product is also registered before the SSA (Mexican Health Secretariat) under registration number Reg SSA
Mex. No 0735 C 88.
According to the manufacturer provided product information:
"VISCOAT Viscoelastic Solution is a sterile, non-pyrogenic, viscoelastic solution of highly purified, non-inflammatory medium molecular weight sodium chondroitin sulfate and sodium hyaluroante. VISCOAT is formulated to a viscosity of 40,000 20,000cps (at shear rate of 2 sec-1, 251C). Each 1mL of VISCOAT solution contains not more than 40mg sodium chondroitin sulfate, 30mg sodium hyaluronate, 0.45mg monobasic sodium phosphate, monohydrate, 2.00mg dibasic sodium phosphate anhydrous, 4.3mg sodium chloride (with Water For Injection, USP
grade, q.s.). The osmolarity of VISCOAT is 325m0sM 40mOsM;
the pH is 7.2 0.2.
Sodium chondroitin sulfate and sodium hyaluronate are quite similar in regard to chemical and physical composition, as each occurs as a large, unbranched chain structure of medium to high molecular weight. The sodium chondroitin sulfate used in the preparation of the VISCOAT Viscoelastic Solution has a mean molecular weight of approximately 22,500 daltons, while the sodium hyaluronate exhibits a molecular weight of over 500,000 daltons."
This product has a gel presentation that contains a mixture of sodium hyaluronate and chondroitin sulfate; these substances exist in natural form in the human body as part of cartilage, synovial membrane, umbilical cord and vitreous humor of the eye.
DETAILED DESCRIPTION OF THE INVENTION
The product is applied or instilled by conventional intraarticular means, typically injection, with prior asepsis and antisepsis of the region. This is typically done in the treatment room of the physician's office. The product applied (injected) is called an implant. As previously explained, CS is the most important part of aggrecan, a predominant proteoglycan in articular cartilage. The CS acts with its long chains inserted in the protein nucleus as a support element of the chondral stroma. It is thus that it serves as an artificial matrix that sticks to the bed of the lesion, allowing the migrating, unattached, peripheral chondrocytes of the erosion to settle in it. When they mature, the chondrocytes secrete a definitive hyaline matrix that replaces the temporary one afforded by the viscoelastic composition("wet nurse") and thus it regenerates until it manages to recover the original shape and thickness. This has been verified when, upon performing controlled post-implant arthroscopy, cartilage regeneration is observed macroscopically. Microscopically, findings of "De Novo" articular cartilage with normal morphological characteristics, as well as positive histophysiological results to S-100 protein, are reported.
The preferred method for this invention's chondrogenic induction is the intraarticular application of a mixture containing 60mg of CS and 45mg of SH in a gel suspension, equivalent to 1.5c.c. of the formulation, when dealing with large joints, and the preferred application of a mixture containing 30mg of CS and 22.5 of SH in 0.75 cubic centimeters for smaller joints such as the coxofemoral, ankle or elbow joints. The inventor has also determined that for even smaller joints such as the interphalangeal or wrist joints, a mixture containing 20mg of CS and 15mg of SH in 0.5 cubic centimeters be used. The number and frequency of administration of the doses of the composition will be similar to those used in conventional viscoprotective therapy regimens, and will be adjusted to suit the needs of the particular patient. Generally, 2 to 6 doses are administered over 1 to 3 months. 2-4 doses administered at 10-20 day intervals are preferred. Most preferred is a regimen of 3 doses at 15 day intervals with subsequent periodic applications every 3, 6, 9, or 12 months, depending on the results. This method produces up to a 94.5% regeneration of the articular cartilage destroyed by grade I and II osteoarthritis, according to the results obtained in the study made on 325 knees and 16 coxofemoral joints.
DETAILED DESCRIPTION OF THE COMPOUND USED IN THE TREATMENT
As mentioned previously, it is important to consider that in the most preferred embodiment of the present invention, the product is applied exactly as it is presented for intraocular use, without any changes in the formulation, using a syringe of.
adequate capacity, for example, a 21 x 32 sterile hypodermic needle. Concentrations of the chondroitin sulfate and hyaluronate useful for purposes of the invention, however, may range from about 0.1% to about 50% by weight for each such component. The molecular weight of the chondroitin sulfate should be greater than about 20,000 daltons, and preferably between about 20,000 and 50,000 daltons. The molecular weight of the hyaluronate should be at least 500,000 daltons and preferably between about 500,000 and 1,000,000 daltons.
In the practice of the invention, sodium hyaluronate may be used at concentrations from about 0.lg up to about 10g in 100ml water at temperatures between about 4 C and about 37 C Chondroitin sulfate is also used at concentrations from about 0.1g up to about lOg in 100ml water at temperatures between about 4 C and about 37 C. Other pharmaceutically acceptable salts, including without limitation magnesium, calcium and potassium chondroitin sulfates and hyaluronates are also useful in the practice of the invention. Within the ranges just described, any quantity of chondroitin sulfate can be added to form binding interaction with hyaluronate and produce physical and flow properties suitable for intraarticular uses. Adding 12.6g of chondroitin sulfate to lOg sodium hyaluronate in water, the resulting solution has viscosity of over 1 million centipoises at 25 C for low shear rate below 50 sec-1). The preferred ratio of chondroitin sulfate to hyaluronate is about 4:3 by weight. Preferred concentrations range from about 0.1% to about 5.3% by weight for the chondroitin sulfate component and from about 0.1% to about 4.2% by weight for the hyaluronate component.
The preferred aqueous buffer solution used in the practice of the invention includes monobasic sodium phosphate, dibasic sodium phosphate, and sodium chloride mixed to form an aqueous buffer to maintain pH of about 7 to about 8.0 and osmolarity of 300-350 mOsmol/kg. By raising the buffer concentration of monobasic sodium phosphate and dibasic sodium phosphate, the ionic strength of chondroitin sulfate/ hyaluronate solution is increased. The kinetic rate constant of molecule interaction between chondroitin sulfate and hyaluronate is increased by raising ionic strength and temperature. This invention comprises concentrations of dibasic sodium phosphate and monobasic sodium phosphate from 0.1g/100ml to 5g/100ml and pH
range of 7.0 to 8.0 at reaction temperatures between 4 C and 40 C. The following example shows the effect of buffer on the viscosity or apparent molecular weight of the mixture for 5.3 g CS/4.2 g SH in 100ml water:
Buffer 1:
Diabasic sodium phosphate: 4.5 mg/ml Sodium dihydrogen phosphate hydrate: 1.5 mg/ml Viscosity of composition of the present invention at 1 sec-t and 25 C is 68,878cps.
Buffer 2:
Dibasic sodium phosphate: 7.5 mg/ml Sodium dihydrogen phosphate hydrate: 1.0 mg/ml Viscosity of compositions of the present invention at.l sec-1 and 25 C is 115, 011cps.
In a most preferred formulation, i.e. the VISCOAT
formulation, each cubic centimeter of the mixture contains 40mg of chondroitin sulfate (molecular weight of approximately 22,500 daltons), 30mg of sodium hyaluronate (molecular weight of approximately 750,000 daltons), 0.45mg of sodium monobasic monohydrate phosphate, 2mg of sodium dibasic anhydrous phosphate, 4.3mg of sodium chloride and water.
TESTS PERFORMED
TYPE OF STUDIES: Prospective, longitudinal and experimental.
A study was conducted on 210 patients, 325 knees with chondromalacia and grades I and II osteoarthritis and 16 joints (coxofemoral) with painful articular symptoms and functional limitation, treated previously in a conventional manner with NSAIDs or with steroid injections; the patients were refractory to these treatments.
INCLUSION CRITERIA
The inclusion criteria during this study were as follows:
Patients of both sexes with chronic chondral or osteochondral pathology of the knee and coxofemoral joint up to grade II arthrosis were included, who had no clinical improvement with conventional treatment, no added autoimmune or neoplastic pathologies, of all ages, with prior arthroscopic surgery, without recent management with systemic or articular steroids or nonsteroidal antiinflammatories (NSAIDs).
EXCLUSION CRITERIA
The following exclusion criteria were adopted: patients with grade III or upper gonarthrosis or coxarthrosis, recent or current treatment with systemic or intraarticular steroids, severe deformities and autoimmune or neoplastic pathology.
NON-INCLUSION CRITERIA
Dropping out of treatment, death, change of medical therapy.
The clinical assessment was as follows:
Pain: slight, moderate or severe.
Gait: occasional claudication, assistance with walking stick or crutches or impossible to walk.
Mobility: complete arches, slight, moderate or severe limitation.
Synovial effusion: minimum, moderate or severe (occasional or constant). (SCRIPPS SCALE FOR SPECIAL SURGERY) Radiographic Assessment:
Radiographic changes, articular clamping, chondromalacia and osteoarthritis. Pre- and posttreatment radiographic studies.
Arthroscopic Assessment:
Pre- and posttreatment images.
RESULTS
210 patients were treated: 325 knees (115 bilateral (230 knees) and 95 unilateral (95 knees), 144 women (68.5%), 66 men (31.5%), aged 12 to 86 years, a mean of 44.2 years, 68 knees were diagnosed with chondromalacia, 40 with grade I
osteoarthritis and 217 with grade II osteoarthritis.
Another 16 patients treated: 16 coxofemoral (hip) joints.
A visual analog clinical scale (SCRIPPS CLINIC FOR SPECIAL
SURGERY) was applied and 309 knees (95.07%) showed immediate significant improvement and satisfactory evolution for up to two years of follow-up, 250 knees (83.3%) remained in the same good conditions without needing to take any NSAID for up to 50 months of follow up, 32 knees (13%)_showed moderate pain and 18 cases (6%) showed no .short and medium term improvement.
Finally, from 16 osteoarthritis coxofemoral (grades I, II, III
and IV). (hip) joints,. 14 .(87.5%) grade I and II c oxofemoral joints showed excellent results, and 2 (12.5%) grade III and IV coxofemoral joints, due to the advanced degree of deterioration, did not. obtain any positive results.
None of the patients had any systemic reactions during this treatment: only one patient reported pain and a slight swelling after the implantation.
previous results, a comparative study To complement the was conducted on 20 patients treated with NSAIDs due to grade II knee osteoarthritis and 20 patients treated with VISCOAT for the same reason. The results were as follows after 90 days:
For pain reduction after 90 days, see Figure 7.
For increased mobility at 90 days, see Figure 8.
Results after 24 months according to the HSS scale (Scripps clinics):
For universe of treated patients and the doses applied, see Figures 9 and 10, respectively.
J
The study continued with the application of 3 doses of the composition to 78 knees, which represented 31% of the knees treated; 151 knees received 2 doses which represented 60.4% and 21 knees received only -one dose which represented 8.4% - The 28%
shown in the graph corresponds to 70 patients. who, after 6 months, needed up to 8 supplementary doses; this has-reduced the index of nocturnal pain, gait pain and pain at rest, and increased. the range of mobility. _ For the doses applied, see Figure 11.
.,= , 19 I
Graphic results of 16 coxofemoral joints, 3 doses covered.87.5%
with excellent results in 14 coxofemoral joints treated with the composition, and the 12.5% shown in the graph corresponds to the 2 patients with grade III and IV osteoarthritis who did not report any positive results.
These applications were made in 9 male and 7 female patients aged 27 to 79 years.
STUDIES AND TESTS PERFORMED ON PATIENTS THAT PROVE CARTILAGE
REGENERATION BY THE APPLICATION OF THE COMPOSITION THAT
CONTAINS CS AND SH.
The following examples are given to illustrate. and demonstrate the new use of the composition of this invention.
065 year old female patient treated previously with the composition of sodium hyaluronate and chondroitin sulfate.
Biopsy of knee cartilage.
MICROSCOPIC DESCRIPTION:
A histological. study of knee cartilage was performed. Its microscopic description was as follows: the sections present fragments of mature cartilage with islands of chondrocyte arranged regularly in groups of 2 to 3, with cohesiveness, and surrounded by a hyaline matrix without laminar fibrosis. The chondr-ocytes have a round nucleus, clear cytoplasm, and they are morphologically normal and with good maturation. There is no endochondral ossification or dystrophic -calcification and, as in the previous example, there is no evidence of malignant neoplasia.
Diagnosis: Biopsy of knee cartilage.
De Novo cartilage formation, morphologica 11y and architecturally normal.
This is confirmed by the image in Figure 3.
78-year -old female patient treated 'previously. with the compound of sodium hyaluronate and chondroitin sulfate. Biopsy of the femoral condylar cartilage.
Microscopic description:
A histologic- study was made of several irregular tissue fragments that jointly measured 0.5 cm; they had a white pearly color, a firm consistency; they were identified as right ti and left. Paraffin technique inclusions were made of them.
Diagnosis: biopsy of femur condylar cartilage.
Fragment of mature cartilage with partial hyalinization.
(Without evidence of malignant neoplasia). See confirmation in the image of Figure 4., Discussion' of the results obtained The functional result subsequent to the implantation of the product was very satisfactory for most of treated patients.
The difference between the plain systemic drug management and the intraarticular application of the chondroitin sulfate and sodium hyaluronate implant is very evidently in favor of the latter. It must be considered that the plain intraarticular rheological change (viscosity, elasticity and plasticity) reduces the pain `and stimulates a synovial response, changing the viscoelastic features of the fluid. However, the basic difference lies in the medium and long term response which may be effected by the chondrogenic induction provoked by CS. and, with it, the permanent solution to the chondral lesion, to the clinical manifestations and.the functional disability, as well as to the risk of major surgery.
Conclusions:
The treatment of osteochondral lesions with intraarticular sodium chondroitin sulfate and sodium hyaluronate has proven to have a significantly favorable clinical response compared with the conventional treatment. This response has been confirmed with pre- and posttreatment arthroscopic imaging, conventional and electron microscope examination as well as histophysiology testing (POSITIVE S-100 Protein) showing that the damaged cartilage is regenerated in a period of about 2 4 years recovering its normal structure, and 'function. The.
indications for the chondrogenic induction intraarticular treatment are preferably addressed to patients with chondrornalacia and grades I and II osteoarthritis in any joint of. the human body.
The. original cause of the osteochondral pathology should invariably be treated, as the long-term result of the procedure will depend on that. Prior surgical management, where indicated, through minimal-invasive surgery, is an excellent alternative for the integral management. of osteochondral lesions and their better long-term prognosis.
Similarly, the methods of the present invention may be used in conjunction with other known therapies. For example, the chondroitin sulfate-hyaluronate mixture of the present invention may be administered to a joint in need thereof in combination with one or more other agents selected from antiinf.lammatories, antirheumatics, steroids and chondrogenic stimulating factors, either separately or in a single formulation-Therefore,, the mixture of sodium' hyaluronate and sodium chondroitin sulfate can be used now in defined amounts in a therapeutically useful manner for all the characterized pathological conditions by the simple intraarticular application route, and the absence of risks of both components makes this therapy particularly attractive.
As. previously discussed, the viscoelastic -compositions of the present invention are known to have utility, in ophthalmic surgery. Those skilled in the viscoelastic arts will appreciate, however, that such compositions will have utility, beyond ophthalmic and joint therapy as described-above. They may be used in a variety of therapies, and especially in drug delivery, cosmetic surgery and reconstructive surgery. The compositions of the present invention are well. suited -for delivery of anti-fibrotics, antibiotics, steroidal and non-steroidal antiinflammatories, anesthetics, analgesics and other medicaments or gene therapies to diseased or traumatized tissues in need thereof. Cosmetically, these compositions may be injected to reduce wrinkles or to treat varicose .veins.
For treatment of dermal lines or wrinkles, these compositions may combined with a muscle relaxing agent such as botulinum toxin type A, commercially available as BOTOS (Allergan, Inc., Irvine CA, USA), and injected subdermally in the conventional manner. The presently disclosed compositions and methods may also be used in any environment where there is a need for tissue separation or stabilization and the potential exists for complications, typically post-surgical, arising from tissue fibrosis and/or adhesions. They will be particularly useful in nasal, spinal cord, cardiovascular, orthopoedic and orthodontic surgical procedures that would otherwise be prone to such complications. -Those skilled in the art will recognize that the preferred modes may' be altered or amended without straying away from the true spirit and scope of the invention as defined in the enclosed claims.
Other, more conventional treatments include anti inf1ammatories, antirheumatics, systemics, physiotherapy, injection of depot steroids and, recently, viscoprotection has emerged.
Viscoprotection involves the intraarticular application of commercially available sodium hyaluronate viscoelastic TM TM TM
materials such as HYLAN c-F 20, SYNVISC, HYALGAN, ARTZ, etc.
The sodium hyaluronate substance does affect the rheology of the synovial fluid, producing an almost immediate sensation of free movement and a marked reduction of pain. It, has been (, proven that the change of the intraarticular fluids attendant to sodium hyaluronate instillation produces a blockage of the nociceptors of subsynovial and capsular tissues and that, in addition to the mechanical factors of the osteochondral pathology, the fluids influence these receptors with their lubricating properties. Thus the change in viscosity of these fluids acts favorably on the painful osteochondral symptoms when sodium hyaluronate is instilled. However, the effect of conventional hyaluronate is temporary because the material remains within the articular chamber for only about 72 hours before it is absorbed and/or metabolized. The residual effects of this substance act on the synovial receptors causing a pain reduction that lasts several weeks and even months.
However, this isolated effect is counterproductive for the course of the disease and for the viability of the cartilage because, as it masks the symptoms, the joint is used with more intensity and its destruction is accelerated as the original problem is not corrected and the the damaged articular cartilage is not restored. Recent studies with a 5 year follow-up with these substances indicate that clinical improvement is significant and that it represents a remission factor of painful symptoms, but only for short and medium term.
Also, adverse effects, characterized by severe pain, significant synovial effusion, rash and ankle edema, have been reported in at least 7.2% of the treated patients. In no instance has hyaluronate therapy been reported to effect cartilage regeneration and long term success. The need exists, therefore, for an improved approach to cartilage regeneration.
As an antecedent to this invention, in 1982, the applicant began applying sodium hyaluronate (SH) to thoroughbred race horses at Hipodromo de las Americas (Las Americas Race Track), in Mexico City, Mexico. The knees and ankles are the most commonly injured joints in these horses. Veterinarians at racetracks in the USA had already used this procedure, observing the beneficial reaction that this viscoelastic material produced in the injured knees of the horses. The applicant considered its use in humans, and conceived of adding some substance to cause the restoration of the damaged surface of the cartilage.
The applicant hypothesized that chondroitin sulfate (CS), the most important part of the aggrecan proteoglycans which are the basis of chondral support, might have a repaving effect.
In 1996 while visiting Alcon Laboratories in Mexico City, the applicant learned that one of the company's ophthalmic products contained both of the above mentioned substances in a gel suspension (VISCOAT ). The inventor obtained detailed information, including the product monograph for VISCOAT that states that is has no reported side effects in intraocular use;
furthermore, there are ample references from efficacy and safety studies of this product.
It was then that the applicant decided to use it experimentally in patients with -osteoarthritis disorders of all degrees, and subsequently analyze the results.
The present study reveals another alternative in the management of osteochondral lesions of the knee through the intraarticular application of a mixture of sodium hyaluronate and sodium chondroitin sulfate. While bound by no theories,- it may be that the remarkable effectiveness of. this therapy is, attributable to the promotion of chondrogenesis s-synergistically combined with the known benefits of viscoelastic therapy.
Implanting an artificial matrix of chondroitin sulfate and sodium hyaluronate may represent an indispensable repair factor, as in it naturally arising chondrocytes can proliferate and restore the continuity of the tissue, regenerating the destroyed cartilage to its original form.
With this matrix, the symptomatic evolution is significantly favorable and long lasting due to the regeneration of cartilage at the chondral lesions. No side effects have been reported except in a patient who reported pain and slight swelling at the site of application, which subsided spontaneously in 24 hours; he was given acetaminophen as an analgesic.
It must be pointed out that in the most preferred usage the product is administered exactly as it is presented for intraocular use and no change is made in the formulation. A
change in presentation with a larger capacity syringe is now being proposed, as the current ophthalmic presentation has 0.5 c.c. and 0.75 c.c. syringes.
It must also be pointed out that although this is the same preparation as that used intraocularly, its use for this purpose is totally different as it is applied in a conventional intraarticular manner as an inductor of chondrogenesis, to regenerate the cartilage destroyed by osteoarthritis.
As previously mentioned, experimental application of this composition in humans started in 1996, and excellent results have been noted. These were confirmed later by arthroscopic studies (direct view of the articular cartilage through the insertion of a camera into the joint), pathological anatomy and histophysiological studies, all of them consistent with the clinical findings that the regeneration of normal articular cartilage was achieved. This is why this treatment is presented as the only currently available procedure that can offer up to 95% regeneration of articular cartilage damaged by grade I and II osteoarthritis in any joint of the human body.
SUMMARY OF THE INVENTION
This invention was developed to solve the problem of the previously available techniques related to the treatment of articular cartilage damaged by osteoarthritis.
This invention introduces a method to achieve regeneration of the articular cartilage by chondrogenic induction through the intraarticular implantation of an artificial matrix in patients and animals with chondromalacia and/or osteoarthritis in any joint, but preferably in human patients with grade I or II osteoarthritis.
The regeneration process is elicited by implanting an artificial matrix formed by a mixture of chondroitin sulfate and hyaluronic acid or pharmaceutically acceptable salts thereof, where naturally arising chondrocytes can settle, and where, as they mature, they form, in groups of 3 or 4, their own definitive hyaline matrix, duplicating the same pattern of the natural cartilage. In this manner the continuity of the articular surface is recovered, mobility is regained, pain is eliminated and thus function is recovered.
The invention also provides a composition adapted to be intraarticularly administered, said composition comprising a mixture of chondroitin sulfate and hyaluronic acid or pharmaceutically acceptable salts thereof for the treatment of degeneration of articular cartilage of a joint.
The invention also provides use of a stable, viscous, buffered aqueous solution which comprises a mixture of chondroitin sulfate at a concentration of about 0.1 to 50% by weight and sodium hyaluronate at a concentration of about 0.1 to 50% by weight in the 8a manufacture of a product for the intraarticular treatment of chondromalacia and/or osteoarthritis.
The invention also provides the use of a mixture of chondroitin sulfate and hyaluronic acid or pharmaceutically acceptable salts thereof for repairing or regenerating cartilage of a mammalian joint characterized by cartilage disease or trauma, said mixture being adapted for intraarticular instillation.
The invention also provides the use of a mixture of chondroitin sulfate and hyaluronic acid or pharmaceutically acceptable salts thereof in the manufacture of a product for intraarticular administration to repair or regenerate cartilage of a mammalian joint characterized by cartilage disease or trauma.
The invention also provides a composition for intraarticular treatment of degeneration of articular cartilage of a joint, the composition comprising a mixture of chondroitin sulfate and hyaluronic acid or pharmaceutically acceptable salts thereof.
The invention also provides a stable, viscous, buffered aqueous solution for the intraarticular treatment of chondromalacia and/or osteoarthritis, the solution comprising a mixture of chondroitin sulfate at a concentration of 0.1 to 50% by weight and sodium hyaluronate at a concentration of 0.1 to 50% by weight in the manufacture of a product.
The invention also provides a mixture for repairing or regenerating cartilage of a mammalian joint characterized by cartilage disease or trauma, the mixture being for intraarticular instillation and the 8b mixture comprising chondroitin sulfate and hyaluronic acid or pharmaceutically acceptable salts thereof.
BRIEF DESCRIPTION OF THE DRAWINGS
Figures 1 to 6 represent pre- and post implantation arthroscopic images from patients who underwent the treatment of the present invention: macro and microscopic confirmation of regeneration of the articular surface with De Novo cartilage with the same characteristics as the original, a conclusive pathology report, and histophysiology tests (S-100 Protein) both of which confirm the characteristics of the regenerated cartilage.
Figures 1 and 2 represent pre- and two years posttreatment arthroscopic images, respectively.
Figure 3 represents the regeneration of the articular cartilage with De Novo cartilage.
Figures 4 and 5 are microscopic images that show the maturing of the zone tangential to the superficial layer where fully developed and maturing chondrocytes are found, surrounded by a hyaline matrix.
Figure 6 is a confirmatory histophysiological image with the application of S-100 protein. It shows the reaction of the cartilaginous tissue to this test with a positive result.
Figures 7 to 11 show .the result of a comparative study of patients treated with NSAIDs and VISCOAT .
CHARACTERISTICS OF THE PRODUCT
In its preferred embodiment, the chondroitin sulfate/hyaluronate composition of the present invention is presented under the trade name of VISCOAT (Alcon Laboratories, Inc., Fort Worth, Texas, USA). It is suitable for the treatment of chondral lesions in all osteoarthritis(OA) in patients, and particularly in those who fail to respond adequately to traditional non-drug therapies and plain analgesics.
The VISCOAT product packaging identifies U.S. Patent Nos.
4,486,416 and 6,051,560. The VISCOAT product is also registered before the SSA (Mexican Health Secretariat) under registration number Reg SSA
Mex. No 0735 C 88.
According to the manufacturer provided product information:
"VISCOAT Viscoelastic Solution is a sterile, non-pyrogenic, viscoelastic solution of highly purified, non-inflammatory medium molecular weight sodium chondroitin sulfate and sodium hyaluroante. VISCOAT is formulated to a viscosity of 40,000 20,000cps (at shear rate of 2 sec-1, 251C). Each 1mL of VISCOAT solution contains not more than 40mg sodium chondroitin sulfate, 30mg sodium hyaluronate, 0.45mg monobasic sodium phosphate, monohydrate, 2.00mg dibasic sodium phosphate anhydrous, 4.3mg sodium chloride (with Water For Injection, USP
grade, q.s.). The osmolarity of VISCOAT is 325m0sM 40mOsM;
the pH is 7.2 0.2.
Sodium chondroitin sulfate and sodium hyaluronate are quite similar in regard to chemical and physical composition, as each occurs as a large, unbranched chain structure of medium to high molecular weight. The sodium chondroitin sulfate used in the preparation of the VISCOAT Viscoelastic Solution has a mean molecular weight of approximately 22,500 daltons, while the sodium hyaluronate exhibits a molecular weight of over 500,000 daltons."
This product has a gel presentation that contains a mixture of sodium hyaluronate and chondroitin sulfate; these substances exist in natural form in the human body as part of cartilage, synovial membrane, umbilical cord and vitreous humor of the eye.
DETAILED DESCRIPTION OF THE INVENTION
The product is applied or instilled by conventional intraarticular means, typically injection, with prior asepsis and antisepsis of the region. This is typically done in the treatment room of the physician's office. The product applied (injected) is called an implant. As previously explained, CS is the most important part of aggrecan, a predominant proteoglycan in articular cartilage. The CS acts with its long chains inserted in the protein nucleus as a support element of the chondral stroma. It is thus that it serves as an artificial matrix that sticks to the bed of the lesion, allowing the migrating, unattached, peripheral chondrocytes of the erosion to settle in it. When they mature, the chondrocytes secrete a definitive hyaline matrix that replaces the temporary one afforded by the viscoelastic composition("wet nurse") and thus it regenerates until it manages to recover the original shape and thickness. This has been verified when, upon performing controlled post-implant arthroscopy, cartilage regeneration is observed macroscopically. Microscopically, findings of "De Novo" articular cartilage with normal morphological characteristics, as well as positive histophysiological results to S-100 protein, are reported.
The preferred method for this invention's chondrogenic induction is the intraarticular application of a mixture containing 60mg of CS and 45mg of SH in a gel suspension, equivalent to 1.5c.c. of the formulation, when dealing with large joints, and the preferred application of a mixture containing 30mg of CS and 22.5 of SH in 0.75 cubic centimeters for smaller joints such as the coxofemoral, ankle or elbow joints. The inventor has also determined that for even smaller joints such as the interphalangeal or wrist joints, a mixture containing 20mg of CS and 15mg of SH in 0.5 cubic centimeters be used. The number and frequency of administration of the doses of the composition will be similar to those used in conventional viscoprotective therapy regimens, and will be adjusted to suit the needs of the particular patient. Generally, 2 to 6 doses are administered over 1 to 3 months. 2-4 doses administered at 10-20 day intervals are preferred. Most preferred is a regimen of 3 doses at 15 day intervals with subsequent periodic applications every 3, 6, 9, or 12 months, depending on the results. This method produces up to a 94.5% regeneration of the articular cartilage destroyed by grade I and II osteoarthritis, according to the results obtained in the study made on 325 knees and 16 coxofemoral joints.
DETAILED DESCRIPTION OF THE COMPOUND USED IN THE TREATMENT
As mentioned previously, it is important to consider that in the most preferred embodiment of the present invention, the product is applied exactly as it is presented for intraocular use, without any changes in the formulation, using a syringe of.
adequate capacity, for example, a 21 x 32 sterile hypodermic needle. Concentrations of the chondroitin sulfate and hyaluronate useful for purposes of the invention, however, may range from about 0.1% to about 50% by weight for each such component. The molecular weight of the chondroitin sulfate should be greater than about 20,000 daltons, and preferably between about 20,000 and 50,000 daltons. The molecular weight of the hyaluronate should be at least 500,000 daltons and preferably between about 500,000 and 1,000,000 daltons.
In the practice of the invention, sodium hyaluronate may be used at concentrations from about 0.lg up to about 10g in 100ml water at temperatures between about 4 C and about 37 C Chondroitin sulfate is also used at concentrations from about 0.1g up to about lOg in 100ml water at temperatures between about 4 C and about 37 C. Other pharmaceutically acceptable salts, including without limitation magnesium, calcium and potassium chondroitin sulfates and hyaluronates are also useful in the practice of the invention. Within the ranges just described, any quantity of chondroitin sulfate can be added to form binding interaction with hyaluronate and produce physical and flow properties suitable for intraarticular uses. Adding 12.6g of chondroitin sulfate to lOg sodium hyaluronate in water, the resulting solution has viscosity of over 1 million centipoises at 25 C for low shear rate below 50 sec-1). The preferred ratio of chondroitin sulfate to hyaluronate is about 4:3 by weight. Preferred concentrations range from about 0.1% to about 5.3% by weight for the chondroitin sulfate component and from about 0.1% to about 4.2% by weight for the hyaluronate component.
The preferred aqueous buffer solution used in the practice of the invention includes monobasic sodium phosphate, dibasic sodium phosphate, and sodium chloride mixed to form an aqueous buffer to maintain pH of about 7 to about 8.0 and osmolarity of 300-350 mOsmol/kg. By raising the buffer concentration of monobasic sodium phosphate and dibasic sodium phosphate, the ionic strength of chondroitin sulfate/ hyaluronate solution is increased. The kinetic rate constant of molecule interaction between chondroitin sulfate and hyaluronate is increased by raising ionic strength and temperature. This invention comprises concentrations of dibasic sodium phosphate and monobasic sodium phosphate from 0.1g/100ml to 5g/100ml and pH
range of 7.0 to 8.0 at reaction temperatures between 4 C and 40 C. The following example shows the effect of buffer on the viscosity or apparent molecular weight of the mixture for 5.3 g CS/4.2 g SH in 100ml water:
Buffer 1:
Diabasic sodium phosphate: 4.5 mg/ml Sodium dihydrogen phosphate hydrate: 1.5 mg/ml Viscosity of composition of the present invention at 1 sec-t and 25 C is 68,878cps.
Buffer 2:
Dibasic sodium phosphate: 7.5 mg/ml Sodium dihydrogen phosphate hydrate: 1.0 mg/ml Viscosity of compositions of the present invention at.l sec-1 and 25 C is 115, 011cps.
In a most preferred formulation, i.e. the VISCOAT
formulation, each cubic centimeter of the mixture contains 40mg of chondroitin sulfate (molecular weight of approximately 22,500 daltons), 30mg of sodium hyaluronate (molecular weight of approximately 750,000 daltons), 0.45mg of sodium monobasic monohydrate phosphate, 2mg of sodium dibasic anhydrous phosphate, 4.3mg of sodium chloride and water.
TESTS PERFORMED
TYPE OF STUDIES: Prospective, longitudinal and experimental.
A study was conducted on 210 patients, 325 knees with chondromalacia and grades I and II osteoarthritis and 16 joints (coxofemoral) with painful articular symptoms and functional limitation, treated previously in a conventional manner with NSAIDs or with steroid injections; the patients were refractory to these treatments.
INCLUSION CRITERIA
The inclusion criteria during this study were as follows:
Patients of both sexes with chronic chondral or osteochondral pathology of the knee and coxofemoral joint up to grade II arthrosis were included, who had no clinical improvement with conventional treatment, no added autoimmune or neoplastic pathologies, of all ages, with prior arthroscopic surgery, without recent management with systemic or articular steroids or nonsteroidal antiinflammatories (NSAIDs).
EXCLUSION CRITERIA
The following exclusion criteria were adopted: patients with grade III or upper gonarthrosis or coxarthrosis, recent or current treatment with systemic or intraarticular steroids, severe deformities and autoimmune or neoplastic pathology.
NON-INCLUSION CRITERIA
Dropping out of treatment, death, change of medical therapy.
The clinical assessment was as follows:
Pain: slight, moderate or severe.
Gait: occasional claudication, assistance with walking stick or crutches or impossible to walk.
Mobility: complete arches, slight, moderate or severe limitation.
Synovial effusion: minimum, moderate or severe (occasional or constant). (SCRIPPS SCALE FOR SPECIAL SURGERY) Radiographic Assessment:
Radiographic changes, articular clamping, chondromalacia and osteoarthritis. Pre- and posttreatment radiographic studies.
Arthroscopic Assessment:
Pre- and posttreatment images.
RESULTS
210 patients were treated: 325 knees (115 bilateral (230 knees) and 95 unilateral (95 knees), 144 women (68.5%), 66 men (31.5%), aged 12 to 86 years, a mean of 44.2 years, 68 knees were diagnosed with chondromalacia, 40 with grade I
osteoarthritis and 217 with grade II osteoarthritis.
Another 16 patients treated: 16 coxofemoral (hip) joints.
A visual analog clinical scale (SCRIPPS CLINIC FOR SPECIAL
SURGERY) was applied and 309 knees (95.07%) showed immediate significant improvement and satisfactory evolution for up to two years of follow-up, 250 knees (83.3%) remained in the same good conditions without needing to take any NSAID for up to 50 months of follow up, 32 knees (13%)_showed moderate pain and 18 cases (6%) showed no .short and medium term improvement.
Finally, from 16 osteoarthritis coxofemoral (grades I, II, III
and IV). (hip) joints,. 14 .(87.5%) grade I and II c oxofemoral joints showed excellent results, and 2 (12.5%) grade III and IV coxofemoral joints, due to the advanced degree of deterioration, did not. obtain any positive results.
None of the patients had any systemic reactions during this treatment: only one patient reported pain and a slight swelling after the implantation.
previous results, a comparative study To complement the was conducted on 20 patients treated with NSAIDs due to grade II knee osteoarthritis and 20 patients treated with VISCOAT for the same reason. The results were as follows after 90 days:
For pain reduction after 90 days, see Figure 7.
For increased mobility at 90 days, see Figure 8.
Results after 24 months according to the HSS scale (Scripps clinics):
For universe of treated patients and the doses applied, see Figures 9 and 10, respectively.
J
The study continued with the application of 3 doses of the composition to 78 knees, which represented 31% of the knees treated; 151 knees received 2 doses which represented 60.4% and 21 knees received only -one dose which represented 8.4% - The 28%
shown in the graph corresponds to 70 patients. who, after 6 months, needed up to 8 supplementary doses; this has-reduced the index of nocturnal pain, gait pain and pain at rest, and increased. the range of mobility. _ For the doses applied, see Figure 11.
.,= , 19 I
Graphic results of 16 coxofemoral joints, 3 doses covered.87.5%
with excellent results in 14 coxofemoral joints treated with the composition, and the 12.5% shown in the graph corresponds to the 2 patients with grade III and IV osteoarthritis who did not report any positive results.
These applications were made in 9 male and 7 female patients aged 27 to 79 years.
STUDIES AND TESTS PERFORMED ON PATIENTS THAT PROVE CARTILAGE
REGENERATION BY THE APPLICATION OF THE COMPOSITION THAT
CONTAINS CS AND SH.
The following examples are given to illustrate. and demonstrate the new use of the composition of this invention.
065 year old female patient treated previously with the composition of sodium hyaluronate and chondroitin sulfate.
Biopsy of knee cartilage.
MICROSCOPIC DESCRIPTION:
A histological. study of knee cartilage was performed. Its microscopic description was as follows: the sections present fragments of mature cartilage with islands of chondrocyte arranged regularly in groups of 2 to 3, with cohesiveness, and surrounded by a hyaline matrix without laminar fibrosis. The chondr-ocytes have a round nucleus, clear cytoplasm, and they are morphologically normal and with good maturation. There is no endochondral ossification or dystrophic -calcification and, as in the previous example, there is no evidence of malignant neoplasia.
Diagnosis: Biopsy of knee cartilage.
De Novo cartilage formation, morphologica 11y and architecturally normal.
This is confirmed by the image in Figure 3.
78-year -old female patient treated 'previously. with the compound of sodium hyaluronate and chondroitin sulfate. Biopsy of the femoral condylar cartilage.
Microscopic description:
A histologic- study was made of several irregular tissue fragments that jointly measured 0.5 cm; they had a white pearly color, a firm consistency; they were identified as right ti and left. Paraffin technique inclusions were made of them.
Diagnosis: biopsy of femur condylar cartilage.
Fragment of mature cartilage with partial hyalinization.
(Without evidence of malignant neoplasia). See confirmation in the image of Figure 4., Discussion' of the results obtained The functional result subsequent to the implantation of the product was very satisfactory for most of treated patients.
The difference between the plain systemic drug management and the intraarticular application of the chondroitin sulfate and sodium hyaluronate implant is very evidently in favor of the latter. It must be considered that the plain intraarticular rheological change (viscosity, elasticity and plasticity) reduces the pain `and stimulates a synovial response, changing the viscoelastic features of the fluid. However, the basic difference lies in the medium and long term response which may be effected by the chondrogenic induction provoked by CS. and, with it, the permanent solution to the chondral lesion, to the clinical manifestations and.the functional disability, as well as to the risk of major surgery.
Conclusions:
The treatment of osteochondral lesions with intraarticular sodium chondroitin sulfate and sodium hyaluronate has proven to have a significantly favorable clinical response compared with the conventional treatment. This response has been confirmed with pre- and posttreatment arthroscopic imaging, conventional and electron microscope examination as well as histophysiology testing (POSITIVE S-100 Protein) showing that the damaged cartilage is regenerated in a period of about 2 4 years recovering its normal structure, and 'function. The.
indications for the chondrogenic induction intraarticular treatment are preferably addressed to patients with chondrornalacia and grades I and II osteoarthritis in any joint of. the human body.
The. original cause of the osteochondral pathology should invariably be treated, as the long-term result of the procedure will depend on that. Prior surgical management, where indicated, through minimal-invasive surgery, is an excellent alternative for the integral management. of osteochondral lesions and their better long-term prognosis.
Similarly, the methods of the present invention may be used in conjunction with other known therapies. For example, the chondroitin sulfate-hyaluronate mixture of the present invention may be administered to a joint in need thereof in combination with one or more other agents selected from antiinf.lammatories, antirheumatics, steroids and chondrogenic stimulating factors, either separately or in a single formulation-Therefore,, the mixture of sodium' hyaluronate and sodium chondroitin sulfate can be used now in defined amounts in a therapeutically useful manner for all the characterized pathological conditions by the simple intraarticular application route, and the absence of risks of both components makes this therapy particularly attractive.
As. previously discussed, the viscoelastic -compositions of the present invention are known to have utility, in ophthalmic surgery. Those skilled in the viscoelastic arts will appreciate, however, that such compositions will have utility, beyond ophthalmic and joint therapy as described-above. They may be used in a variety of therapies, and especially in drug delivery, cosmetic surgery and reconstructive surgery. The compositions of the present invention are well. suited -for delivery of anti-fibrotics, antibiotics, steroidal and non-steroidal antiinflammatories, anesthetics, analgesics and other medicaments or gene therapies to diseased or traumatized tissues in need thereof. Cosmetically, these compositions may be injected to reduce wrinkles or to treat varicose .veins.
For treatment of dermal lines or wrinkles, these compositions may combined with a muscle relaxing agent such as botulinum toxin type A, commercially available as BOTOS (Allergan, Inc., Irvine CA, USA), and injected subdermally in the conventional manner. The presently disclosed compositions and methods may also be used in any environment where there is a need for tissue separation or stabilization and the potential exists for complications, typically post-surgical, arising from tissue fibrosis and/or adhesions. They will be particularly useful in nasal, spinal cord, cardiovascular, orthopoedic and orthodontic surgical procedures that would otherwise be prone to such complications. -Those skilled in the art will recognize that the preferred modes may' be altered or amended without straying away from the true spirit and scope of the invention as defined in the enclosed claims.
Claims (39)
1. Use of a composition for intraarticular administration, said composition comprising a mixture of chondroitin sulfate and hyaluronic acid or pharmaceutically acceptable salts thereof, for the treatment of degeneration of articular cartilage of a joint.
2. The use of claim 1, wherein the degeneration of the cartilage is caused by chondromalacia or osteoarthritis of grade I or grade II.
3. The use of claim 1 or claim 2, wherein the composition comprises a mixture of chondroitin sulfate at a concentration of 0.1 to 50% by weight and sodium hyaluronate at a concentration of 0.1 to 50% by weight.
4. The use of claim 3, wherein the chondroitin sulfate concentration is from 0.1 to 3.3% by weight and the sodium hyaluronate concentration is from 0.1 to 4.2% by weight of the composition.
5. The use of claim 4, wherein the composition is a viscoelastic solution comprising a mixture of chondroitin sulfate and sodium hyaluronate in a ratio of about 4 parts by weight chondroitin sulfate to about 3 parts by weight sodium hyaluronate.
6. The use of claim 5, wherein the concentration of chondroitin sulfate is about 40 mg/ml and the concentration of sodium hyaluronate is about 30 mg/ml.
7. The use of claim 6, wherein the chondroitin sulfate has a molecular weight of 20,000 to 50,000 daltons, the sodium hyaluronate has a molecular weight of 500,000 to 1,000,000 daltons, and the composition has a viscosity of 20,000 to 60,000 cps.
8. The use of claim 1, wherein the composition is a viscoelastic composition and doses of between 0.5 and 1.5 cubic centimeters of the viscoelastic composition are adapted to be administered to the affected joint.
9. The use of claim 8, wherein 2 to 6 doses of the viscoelastic composition are adapted to be administered over 1 to 3 months.
10. The use of claim 9, wherein 2 to 4 doses of the viscoelastic composition are adapted to be administered at 10 to 20 day intervals.
11. The use of claim 10, wherein 3 doses of the viscoelastic composition are adapted to be administered at 15 day intervals.
12. The use according to claim 8, wherein the joint is a human joint that is a knee, a shoulder, a sacroiliac joint;
a coxofemoral joint, an ankle, an elbow, an interphalangeal joint or a wrist.
a coxofemoral joint, an ankle, an elbow, an interphalangeal joint or a wrist.
13. The use according to claim 12, wherein each dose of the viscoelastic composition is 1.5 cubic centimeters of the composition for the knee, shoulder or sacroiliac joints.
14. The use according to claim 8, wherein each dose of the viscoelastic composition is 0.75 cubic centimeters of the composition for the coxofemoral, ankle or elbow joints.
15. The use according to claim 8, wherein each dose of the viscoelastic composition is 0.5 cubic centimeters of the composition for the interphalangeal or wrist joints.
16. Use of a stable, viscous, buffered aqueous solution which comprises a mixture of chondroitin sulfate at a concentration of 0.1 to 50% by weight and sodium hyaluronate at a concentration of 0.1 to 50% by weight in the manufacture of a product for the intraarticular treatment of chondromalacia and/or osteoarthritis.
17. The use according to claim 16, wherein said solution is buffered at a pH of 7.0 to 8Ø
18. The use according to claim 16 or claim 17, wherein the solution has an osmolarity of from 300 to 350 mOsmol/kg.
19. Use of a mixture of chondroitin sulfate and hyaluronic acid or pharmaceutically acceptable salts thereof for repairing or regenerating cartilage of a mammalian joint characterized by cartilage disease or trauma, said mixture being for intraarticular instillation.
20. Use of a mixture of chondroitin sulfate and hyaluronic acid or pharmaceutically acceptable salts thereof in the manufacture of a product for intraarticular administration to repair or regenerate cartilage of a mammalian joint characterized by cartilage disease or trauma.
21. A composition for intraarticular treatment of degeneration of articular cartilage of a joint, said composition comprising a mixture of chondroitin sulfate and hyaluronic acid or pharmaceutically acceptable salts thereof.
22. The composition of claim 21, wherein the degeneration of the cartilage is caused by chondromalacia or osteoarthritis of grade I or grade II.
23. The composition of claim 21 or 22, wherein the composition comprises a mixture of chondroitin sulfate at a concentration of 0.1 to 50% by weight and sodium hyaluronate at a concentration of 0.1 to 50% by weight.
24. The composition of claim 23, wherein the chondroitin sulfate concentration is from 0.1 to 5.3% by weight and the sodium hyaluronate concentration is from 0.1 to 4.2% by weight of the composition.
25. The composition of claim 24, wherein the composition is a viscoelastic solution comprising a mixture of chondroitin sulfate and sodium hyaluronate in a ratio of about 4 parts by weight chondroitin sulfate to about 3 parts by weight sodium hyaluronate.
26. The composition of claim 25, wherein the concentration of chondroitin sulfate is about 40 mg/ml and the concentration of sodium hyaluronate is about 30 mg/ml.
27. The composition of claim 26, wherein the chondroitin sulfate has a molecular weight of 20,000 to 50,000 daltons, the sodium hyaluronate has a molecular weight of 500,000 to 1,000,000 daltons, and the composition has a viscosity of 20,000 to 60,000 cps.
28. The composition of claim 21, wherein the composition is a viscoelastic composition and doses of between 0.5 and 1.5 cubic centimeters of the viscoelastic composition are adapted to be administered to the affected joint.
29. The composition of claim 28, wherein 2 to 6 doses of the viscoelastic composition are adapted to be administered over 1 to 3 months.
30. The composition of claim 29, wherein 2 to 4 doses of the viscoelastic composition are adapted to be administered at to 20 day intervals.
31. The composition of claim 30, wherein 3 doses of the viscoelastic composition are adapted to be administered at day intervals.
32. The composition according to claim 28, wherein the joint is a human joint that is a knee, a shoulder, a sacroiliac joint; a coxofemoral joint, an ankle, an elbow, an interphalangeal joint or a wrist.
33. The composition according to claim 32, wherein each dose of the viscoelastic composition is 1.5 cubic centimeters of the composition for the knee, shoulder or sacroiliac joints.
34. The composition according to claim 28, wherein each dose of the viscoelastic composition is 0.75 cubic centimeters of the composition for the coxofemoral, ankle or elbow joints.
35. The composition according to claim 28, wherein each dose of the viscoelastic composition is 0.5 cubic centimeters of the composition for the interphalangeal or wrist joints.
36. A stable, viscous, buffered aqueous solution for the intraarticular treatment of chondromalacia and/or osteoarthritis, the solution comprising a mixture of chondroitin sulfate at a concentration of 0.1 to 50% by weight and sodium hyaluronate at a concentration of 0.1 to 50% by weight in the manufacture of a product.
37. The solution according to claim 36 being buffered at a pH of 7.0 to 8Ø
38. The solution according to claim 36 or 37 having an osmolarity of from 300 to 350 mOsmol/kg.
39. A mixture for repairing or regenerating cartilage of a mammalian joint characterized by cartilage disease or trauma, said mixture being for intraarticular instillation and said mixture comprising chondroitin sulfate and hyaluronic acid or pharmaceutically acceptable salts thereof.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
MXPA/A/2001/011542 | 2001-11-13 | ||
MXPA01011542A MXPA01011542A (en) | 2001-11-13 | 2001-11-13 | Regeneration of articular cartilage damaged by osteoarthritis i and ii, by means of intra-articular application of sodium hyaluronate and chondroitin sulphate in a gel carrier. |
US10/082,743 US6906044B2 (en) | 2001-11-13 | 2002-02-22 | Regeneration of articular cartilage damaged by grade I and II osteoarthritis by means of the intraarticular application of a mixture of sodium hyaluronate and chondroitin sulfate in a gel vehicle |
US10/082,743 | 2002-02-22 | ||
PCT/EP2002/012703 WO2003041724A1 (en) | 2001-11-13 | 2002-11-13 | Use of a mixture of sodium hyaluronate and chondroitin sulfate for the treatment of osteoarthritis |
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CA2466499A1 CA2466499A1 (en) | 2003-05-22 |
CA2466499C true CA2466499C (en) | 2011-10-18 |
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CA2466499A Expired - Fee Related CA2466499C (en) | 2001-11-13 | 2002-11-13 | Use of a mixture of sodium hyaluronate and chondroitin sulfate for the treatment of osteoarthritis |
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Families Citing this family (132)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8632785B2 (en) * | 2000-02-08 | 2014-01-21 | Allergan, Inc. | Clostridial toxin pharmaceutical composition containing a gelatin fragment |
US20060269575A1 (en) * | 2000-02-08 | 2006-11-30 | Allergan, Inc. | Botulinum toxin pharmaceutical compositions formulated with recombinant albumin |
EP1586329A1 (en) * | 2000-02-08 | 2005-10-19 | Allergan, Inc. | Botulinum toxin pharmaceutical compositions |
US7780967B2 (en) * | 2000-02-08 | 2010-08-24 | Allergan, Inc. | Reduced toxicity Clostridial toxin pharmaceutical compositions |
US20030118598A1 (en) * | 2000-02-08 | 2003-06-26 | Allergan, Inc. | Clostridial toxin pharmaceutical compositions |
US20030086899A1 (en) * | 2000-03-14 | 2003-05-08 | Jafari Masoud R. | Chondroitin sulfate containing viscoelastics for use in treating joints |
US20040118414A1 (en) | 2002-12-20 | 2004-06-24 | Shah Mandar V | Surface modified viscoelastics for ocular surgery |
US20040101561A1 (en) * | 2002-11-13 | 2004-05-27 | Jafari Masoud R. | Combinations of viscoelastics for use during surgery |
US7363928B2 (en) | 2001-12-21 | 2008-04-29 | Alcon, Inc. | Dilution resistant viscoelastic compositions |
US7820194B2 (en) * | 2001-12-21 | 2010-10-26 | Alcon, Inc. | Combinations of viscoelastics for use during surgery |
US7691394B2 (en) | 2002-05-28 | 2010-04-06 | Botulinum Toxin Research Associates, Inc. | High-potency botulinum toxin formulations |
US7803787B2 (en) * | 2002-10-16 | 2010-09-28 | Arthrodynamic Technologies, Animal Health Division, Inc. | Composition and method for treating connective tissue damage by transmucosal administration |
US8455458B2 (en) | 2002-10-16 | 2013-06-04 | Arthrodynamic Technologies, Animal Health Division, Inc. | Composition and method for treating connective tissue damage |
US7504387B2 (en) * | 2002-10-16 | 2009-03-17 | Arthrodynamic Technologies, Animal Health Division, Inc. | Glycosaminoglycan composition and method for treatment and prevention of interstitial cystitis |
US7485629B2 (en) * | 2002-10-16 | 2009-02-03 | Arthrodynamic Technologies, Animal Health Division, Inc. | Composition and method for treatment of joint damage |
US20080003258A1 (en) * | 2002-10-16 | 2008-01-03 | Marcum Frank D | Composition and Method for Treating Rheumatoid Arthritis |
KR20050094817A (en) * | 2002-12-20 | 2005-09-28 | 보툴리늄 톡신 리서치 어쏘시에이츠, 인크. | Improved pharmaceutical botulinum toxin compositions |
JP2007507516A (en) * | 2003-10-01 | 2007-03-29 | オプティマー・ファーマスーティカルズ・インコーポレイテッド | Treatment of mammalian disorders by amino sugar administration and use of amino sugar |
SI1677806T1 (en) * | 2003-10-09 | 2009-06-30 | Robert John Petrella | Methods for treating acute and overuse sprain and strain using hyaluronic acid |
DE102004019241A1 (en) * | 2004-04-16 | 2005-11-03 | Cellmed Ag | Injectable cross-linked and uncrosslinked alginates and their use in medicine and aesthetic surgery |
US20060003964A1 (en) * | 2004-06-30 | 2006-01-05 | Shah Mandar V | Dilution resistant viscoelastic compositions |
US20060073208A1 (en) * | 2004-10-01 | 2006-04-06 | Allergan, Inc. | Cosmetic neurotoxin compositions and methods |
DE102004055729A1 (en) * | 2004-11-18 | 2006-05-24 | Cellmed Ag | Production of two or more layered microcapsules |
WO2006058109A1 (en) * | 2004-11-23 | 2006-06-01 | Alcon, Inc. | Triple natural polymer viscoelastic composition |
SI1835923T1 (en) * | 2004-12-30 | 2014-01-31 | Genzyme Coroporation | Regimens for intra-articular viscosupplementation |
WO2007008206A1 (en) * | 2005-07-11 | 2007-01-18 | Alcon, Inc. | Dilution resistant viscoelastic compositions |
US8323666B2 (en) * | 2005-08-01 | 2012-12-04 | Allergan, Inc. | Botulinum toxin compositions |
JP4875865B2 (en) * | 2005-08-17 | 2012-02-15 | 株式会社日本バリアフリー | NTx value reducing agent for prevention and treatment of osteoarthritis |
ES2281265B1 (en) * | 2005-11-24 | 2008-08-16 | Bioiberica, S.A. | COMPOSITIONS FOR THE TREATMENT OF ARTROSIS. |
ITMI20061030A1 (en) | 2006-05-26 | 2007-11-27 | Altergon Sa | NEW COMPOSITION INCLUDING GLYCOSAMINOGLICANS WITH CONTROLLED VISCOSITY AND USE OF SUCH COMPOSITION IN THE THERAPY OF CHRONIC CYSTITIS |
TW200743492A (en) * | 2006-05-29 | 2007-12-01 | Ching-Chuan Jiang | Hyaluronic acid used for purpose of anti-oxidation and proliferation on cartilage cells |
ITPD20060219A1 (en) | 2006-05-31 | 2007-12-01 | Fidia Farmaceutici | PHARMACEUTICAL COMPOSITIONS CONTAINING HYALURONIC ACID SULFATED IN THE TREATMENT OF OSTEOARTHROSIS |
CN100417415C (en) * | 2006-06-28 | 2008-09-10 | 文彦春 | Soakage of medication for treating arthritis |
US20080069855A1 (en) * | 2006-08-21 | 2008-03-20 | Bonutti Peter M | Method of inhibiting the formation of adhesions and scar tissue and reducing blood loss |
ES2304886B1 (en) * | 2007-04-13 | 2009-11-11 | Bioiberica, S.A. | "COMPOSITIONS TO NUTRIR THE ARTICULATIONS". |
US9186375B2 (en) | 2007-06-21 | 2015-11-17 | Arthrodynamic Technologies, Animal Health Division, Inc. | Glycosaminoglycan compositions in combination with stem cells |
CA3115223A1 (en) * | 2007-07-10 | 2011-01-13 | Implantica Patent Ltd. | Hip joint device and method |
US8394782B2 (en) * | 2007-11-30 | 2013-03-12 | Allergan, Inc. | Polysaccharide gel formulation having increased longevity |
US9044477B2 (en) * | 2007-12-12 | 2015-06-02 | Allergan, Inc. | Botulinum toxin formulation |
US9161970B2 (en) | 2007-12-12 | 2015-10-20 | Allergan, Inc. | Dermal filler |
EP2082755A1 (en) * | 2008-01-16 | 2009-07-29 | CellMed AG | Monolithic alginate implants networked in situ |
US8828354B2 (en) | 2008-03-27 | 2014-09-09 | Warsaw Orthopedic, Inc. | Pharmaceutical gels and methods for delivering therapeutic agents to a site beneath the skin |
US9072727B2 (en) | 2008-04-18 | 2015-07-07 | Warsaw Orthopedic, Inc. | Alpha adrenergic receptor agonists for treatment of degenerative disc disease |
US8420114B2 (en) * | 2008-04-18 | 2013-04-16 | Warsaw Orthopedic, Inc. | Alpha and beta adrenergic receptor agonists for treatment of pain and / or inflammation |
US9132085B2 (en) | 2008-04-18 | 2015-09-15 | Warsaw Orthopedic, Inc. | Compositions and methods for treating post-operative pain using clonidine and bupivacaine |
US20090263451A1 (en) * | 2008-04-18 | 2009-10-22 | Warsaw Orthopedic, Inc. | Anti-Inflammatory and/or Analgesic Agents for Treatment of Myofascial Pain |
US20090264477A1 (en) * | 2008-04-18 | 2009-10-22 | Warsaw Orthopedic, Inc., An Indiana Corporation | Beta adrenergic receptor agonists for treatment of pain and/or inflammation |
US8629172B2 (en) | 2008-04-18 | 2014-01-14 | Warsaw Orthopedic, Inc. | Methods and compositions for treating post-operative pain comprising clonidine |
USRE48948E1 (en) | 2008-04-18 | 2022-03-01 | Warsaw Orthopedic, Inc. | Clonidine compounds in a biodegradable polymer |
US20090264489A1 (en) * | 2008-04-18 | 2009-10-22 | Warsaw Orthopedic, Inc. | Method for Treating Acute Pain with a Formulated Drug Depot in Combination with a Liquid Formulation |
US8889173B2 (en) * | 2008-04-18 | 2014-11-18 | Warsaw Orthopedic, Inc. | Alpha adrenergic receptor agonists for treatment of pain and/or inflammation |
US8557273B2 (en) | 2008-04-18 | 2013-10-15 | Medtronic, Inc. | Medical devices and methods including polymers having biologically active agents therein |
US9132119B2 (en) | 2008-04-18 | 2015-09-15 | Medtronic, Inc. | Clonidine formulation in a polyorthoester carrier |
US9125917B2 (en) * | 2008-04-18 | 2015-09-08 | Warsaw Orthopedic, Inc. | Fluocinolone formulations in a biodegradable polymer carrier |
US9289409B2 (en) * | 2008-04-18 | 2016-03-22 | Warsaw Orthopedic, Inc. | Sulindac formulations in a biodegradable material |
US8883768B2 (en) * | 2008-04-18 | 2014-11-11 | Warsaw Orthopedic, Inc. | Fluocinolone implants to protect against undesirable bone and cartilage destruction |
US20090263443A1 (en) * | 2008-04-18 | 2009-10-22 | Warsaw Orthopedics, Inc. | Methods for treating post-operative effects such as spasticity and shivering with clondine |
US8956641B2 (en) | 2008-04-18 | 2015-02-17 | Warsaw Orthopedic, Inc. | Alpha adrenergic receptor agonists for treatment of inflammatory diseases |
US8956636B2 (en) | 2008-04-18 | 2015-02-17 | Warsaw Orthopedic, Inc. | Methods and compositions for treating postoperative pain comprosing ketorolac |
US8846068B2 (en) | 2008-04-18 | 2014-09-30 | Warsaw Orthopedic, Inc. | Methods and compositions for treating post-operative pain comprising a local anesthetic |
US8722079B2 (en) | 2008-04-18 | 2014-05-13 | Warsaw Orthopedic, Inc. | Methods for treating conditions such as dystonia and post-stroke spasticity with clonidine |
CN102065929B (en) | 2008-06-26 | 2013-11-06 | 凯希特许有限公司 | System for stimulation of cartilage formation using reduced pressure treatment |
US20100015049A1 (en) * | 2008-07-16 | 2010-01-21 | Warsaw Orthopedic, Inc. | Methods and compositions for treating postoperative pain comprising nonsteroidal anti-inflammatory agents |
US9492375B2 (en) | 2008-07-23 | 2016-11-15 | Warsaw Orthopedic, Inc. | Foam carrier for bone grafting |
US9161903B2 (en) * | 2008-10-31 | 2015-10-20 | Warsaw Orthopedic, Inc. | Flowable composition that hardens on delivery to a target tissue site beneath the skin |
EP2364168A1 (en) | 2008-12-04 | 2011-09-14 | Botulinum Toxin Research Associates, Inc. | Extended length botulinum toxin formulation for human or mammalian use |
KR20110107357A (en) * | 2008-12-30 | 2011-09-30 | 케이씨아이 라이센싱 인코포레이티드 | Reduced pressure augmentation of microfracture procedures for cartilage repair |
US20100228097A1 (en) * | 2009-03-04 | 2010-09-09 | Warsaw Orthopedic, Inc. | Methods and compositions to diagnose pain |
US20100226959A1 (en) * | 2009-03-04 | 2010-09-09 | Warsaw Orthopedic, Inc. | Matrix that prolongs growth factor release |
US20100239632A1 (en) * | 2009-03-23 | 2010-09-23 | Warsaw Orthopedic, Inc. | Drug depots for treatment of pain and inflammation in sinus and nasal cavities or cardiac tissue |
IT1393945B1 (en) | 2009-04-21 | 2012-05-17 | Fidia Farmaceutici | COMPOSITIONS INCLUDING HYALURONIC ACID, HYALURONIC ACID, SULFATE, CALCIUM AND VITAMIN D3 IN THE TREATMENT OF OSTEOARTICULAR AND MUSCULOSCHELETAL DISEASES |
US8617583B2 (en) | 2009-07-17 | 2013-12-31 | Warsaw Orthopedic, Inc. | Alpha adrenergic receptor agonists for prevention or treatment of a hematoma, edema, and/or deep vein thrombosis |
US8231891B2 (en) | 2009-07-31 | 2012-07-31 | Warsaw Orthopedic, Inc. | Implantable drug depot for weight control |
CN101690728B (en) * | 2009-09-23 | 2011-09-21 | 沈阳亿灵医药科技有限公司 | Composition for treating osteoarthritis |
US20110097375A1 (en) | 2009-10-26 | 2011-04-28 | Warsaw Orthopedic, Inc. | Formulation for preventing or reducing bleeding at a surgical site |
US20110097380A1 (en) * | 2009-10-28 | 2011-04-28 | Warsaw Orthopedic, Inc. | Clonidine formulations having antimicrobial properties |
US9504698B2 (en) * | 2009-10-29 | 2016-11-29 | Warsaw Orthopedic, Inc. | Flowable composition that sets to a substantially non-flowable state |
US8597192B2 (en) | 2009-10-30 | 2013-12-03 | Warsaw Orthopedic, Inc. | Ultrasonic devices and methods to diagnose pain generators |
US8758791B2 (en) | 2010-01-26 | 2014-06-24 | Warsaw Orthopedic, Inc. | Highly compression resistant matrix with porous skeleton |
US8475824B2 (en) | 2010-01-26 | 2013-07-02 | Warsaw Orthopedic, Inc. | Resorbable matrix having elongated particles |
US9125902B2 (en) * | 2010-01-28 | 2015-09-08 | Warsaw Orthopedic, Inc. | Methods for treating an intervertebral disc using local analgesics |
US9050274B2 (en) * | 2010-01-28 | 2015-06-09 | Warsaw Orthopedic, Inc. | Compositions and methods for treating an intervertebral disc using bulking agents or sealing agents |
US9486500B2 (en) | 2010-01-28 | 2016-11-08 | Warsaw Orthopedic, Inc. | Osteoimplant and methods for making |
US8246571B2 (en) | 2010-08-24 | 2012-08-21 | Warsaw Orthopedic, Inc. | Drug storage and delivery device having a retaining member |
US9414930B2 (en) | 2010-10-26 | 2016-08-16 | Kyphon SÀRL | Activatable devices containing a chemonucleolysis agent |
US8404268B2 (en) | 2010-10-26 | 2013-03-26 | Kyphon Sarl | Locally targeted anti-fibrotic agents and methods of use |
US8740982B2 (en) | 2010-10-26 | 2014-06-03 | Kyphon Sarl | Devices containing a chemonucleolysis agent and methods for treating an intervertebral disc or spinal arachnoiditis |
WO2012075451A2 (en) | 2010-12-03 | 2012-06-07 | Warsaw Orthopedic, Inc. | Clonidine and gaba compounds in a biodegradable polymer carrier |
WO2012075447A2 (en) | 2010-12-03 | 2012-06-07 | Warsaw Orthopedic, Inc. | Compositions and methods for delivering clonidine and bupivacaine to a target tissue site |
US20120142628A1 (en) * | 2010-12-07 | 2012-06-07 | Allergan, Inc. | Methods for treating crepitus |
US8398611B2 (en) * | 2010-12-28 | 2013-03-19 | Depuy Mitek, Inc. | Compositions and methods for treating joints |
US8455436B2 (en) | 2010-12-28 | 2013-06-04 | Depuy Mitek, Llc | Compositions and methods for treating joints |
US8524662B2 (en) | 2010-12-28 | 2013-09-03 | Depuy Mitek, Llc | Compositions and methods for treating joints |
US9060978B2 (en) | 2011-01-24 | 2015-06-23 | Warsaw Orthopedic, Inc. | Method for treating an intervertebral disc disorder by administering a dominant negative tumor necrosis factor antagonist |
US9717779B2 (en) | 2011-01-31 | 2017-08-01 | Warsaw Orthopedic, Inc. | Implantable matrix having optimum ligand concentrations |
US9592243B2 (en) | 2011-04-25 | 2017-03-14 | Warsaw Orthopedic, Inc. | Medical devices and methods comprising an anabolic agent for treatment of an injury |
US9511077B2 (en) | 2011-04-25 | 2016-12-06 | Warsaw Orthopedic, Inc. | Medical devices and methods comprising an anabolic agent for wound healing |
US9186377B2 (en) | 2011-06-03 | 2015-11-17 | Maguire Abbey, Llc | Method, composition, and articles for improving joint lubrication |
US8623839B2 (en) * | 2011-06-30 | 2014-01-07 | Depuy Mitek, Llc | Compositions and methods for stabilized polysaccharide formulations |
US9205241B2 (en) | 2011-07-12 | 2015-12-08 | Warsaw Orthopedic, Inc. | Medical devices and methods comprising an adhesive material |
US9132194B2 (en) | 2011-07-12 | 2015-09-15 | Warsaw Orthopedic, Inc. | Medical devices and methods comprising an adhesive sheet containing a drug depot |
PL2596796T3 (en) | 2011-11-24 | 2014-04-30 | Quimera Ingenieria Biomedica S L | Complex obtained from hyaluronic acid or a salt thereof and chondroitin sulphate mixtures |
ITPD20120098A1 (en) * | 2012-03-30 | 2013-10-01 | Fidia Farmaceutici | "NEW PHARAMACEUTICAL FORMULATIONS CONTAINING CONDROITIN SULFATE AND DERIVATIVES OF HYALURONIC ACID" |
US9511018B2 (en) | 2012-04-05 | 2016-12-06 | Warsaw Orthopedic, Inc. | Clonidine compounds in a biodegradable matrix |
US9393291B2 (en) | 2012-04-12 | 2016-07-19 | Botulinum Toxin Research Associates, Inc. | Use of botulinum toxin for the treatment of cerebrovascular disease, renovascular and retinovascular circulatory beds |
US8735504B2 (en) | 2012-05-02 | 2014-05-27 | Warsaw Orthopedic, Inc. | Methods for preparing polymers having low residual monomer content |
US9180223B2 (en) | 2012-05-10 | 2015-11-10 | The Trustees Of The Stevens Institute Of Technology | Biphasic osteochondral scaffold for reconstruction of articular cartilage |
FR2991876B1 (en) | 2012-06-13 | 2014-11-21 | Vivacy Lab | COMPOSITION, IN AQUEOUS MEDIUM, COMPRISING AT LEAST ONE HYALURONIC ACID AND AT LEAST ONE WATER-SOLUBLE SALT OF SUCROSE OCTASULFATE |
PT2900247T (en) * | 2012-09-26 | 2018-03-28 | Bone Therapeutics Sa | Compositions comprising solvent/detergent-treated plasma and hyaluronic acid for use in the treatment of muskuloskeletal disorders |
US9066853B2 (en) | 2013-01-15 | 2015-06-30 | Warsaw Orthopedic, Inc. | Clonidine compounds in a biodegradable fiber |
EP2886104A1 (en) | 2013-12-11 | 2015-06-24 | Patir, Suleyman | An intra-articular gel |
US9480731B2 (en) * | 2013-12-12 | 2016-11-01 | Medy-Tox, Inc. | Long lasting effect of new botulinum toxin formulations |
ITMI20132116A1 (en) | 2013-12-18 | 2015-06-19 | Apharm Srl | ASSOCIATION OF GLYCOSAMINOGLICANS AND CYCLODESTRINES |
EP3760186A1 (en) * | 2014-04-30 | 2021-01-06 | Allergan, Inc. | Formulations of biologics for intravesical instillation |
US11484580B2 (en) | 2014-07-18 | 2022-11-01 | Revance Therapeutics, Inc. | Topical ocular preparation of botulinum toxin for use in ocular surface disease |
US9901627B2 (en) | 2014-07-18 | 2018-02-27 | Revance Therapeutics, Inc. | Topical ocular preparation of botulinum toxin for use in ocular surface disease |
US10080877B2 (en) | 2014-07-25 | 2018-09-25 | Warsaw Orthopedic, Inc. | Drug delivery device and methods having a drug cartridge |
US9775978B2 (en) | 2014-07-25 | 2017-10-03 | Warsaw Orthopedic, Inc. | Drug delivery device and methods having a retaining member |
EP3000489B1 (en) | 2014-09-24 | 2017-04-05 | Sofradim Production | Method for preparing an anti-adhesion barrier film |
US9682099B2 (en) | 2015-01-20 | 2017-06-20 | DePuy Synthes Products, Inc. | Compositions and methods for treating joints |
CN105982912A (en) * | 2015-03-02 | 2016-10-05 | 黄绣川 | Pharmaceutical composition containing sodium hyaluronate and chondroitin sulfate |
EP3111941A1 (en) | 2015-06-30 | 2017-01-04 | Süleyman Patir | Compositions comprising chondroitin sulfate for prophylaxis or treatment of damaged articular cartilage |
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US10076650B2 (en) | 2015-11-23 | 2018-09-18 | Warsaw Orthopedic, Inc. | Enhanced stylet for drug depot injector |
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USD802755S1 (en) | 2016-06-23 | 2017-11-14 | Warsaw Orthopedic, Inc. | Drug pellet cartridge |
JP7217700B2 (en) | 2016-09-13 | 2023-02-03 | アラーガン、インコーポレイテッド | Stabilized non-protein Clostridial toxin composition |
US10434261B2 (en) | 2016-11-08 | 2019-10-08 | Warsaw Orthopedic, Inc. | Drug pellet delivery system and method |
KR102364483B1 (en) * | 2019-07-04 | 2022-02-21 | 백우인 | Nutrition material for pets with natural materials |
CN111955722A (en) * | 2020-08-05 | 2020-11-20 | 山东明仁福瑞达制药股份有限公司 | Medical nutrition meal composition beneficial to medicine absorption and recovery and used for rehabilitation of osteopathic patients and medical nutrition meal |
CN112245395A (en) * | 2020-11-20 | 2021-01-22 | 佳木斯大学 | Medical cartilage repairing agent and preparation method thereof |
US11318187B1 (en) * | 2021-09-02 | 2022-05-03 | Bjorn Eek | Treatment of internal disc disruption and connective tissue injuries |
Family Cites Families (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4486416A (en) | 1981-03-02 | 1984-12-04 | Soll David B | Protection of human and animal cells subject to exposure to trauma |
US4971955A (en) | 1981-03-02 | 1990-11-20 | Soll David B | Protection of human and animal cells during surgical trauma |
US5409904A (en) | 1984-11-13 | 1995-04-25 | Alcon Laboratories, Inc. | Hyaluronic acid compositions and methods |
US5340579A (en) * | 1984-11-20 | 1994-08-23 | Farmaka S.R.L. | Method of treating dermatological conditions |
US4636524A (en) | 1984-12-06 | 1987-01-13 | Biomatrix, Inc. | Cross-linked gels of hyaluronic acid and products containing such gels |
US4713448A (en) | 1985-03-12 | 1987-12-15 | Biomatrix, Inc. | Chemically modified hyaluronic acid preparation and method of recovery thereof from animal tissues |
US5099013A (en) | 1985-03-12 | 1992-03-24 | Biomatrix, Inc, | Hylan preparation and method of recovery thereof from animal tissues |
SE8501723L (en) * | 1985-04-09 | 1986-10-10 | Pharmacia Ab | PREPARATION TO BE USED IN TREATMENT OF LED INFLAMMATION |
US6051560A (en) * | 1986-06-26 | 2000-04-18 | Nestle S.A. | Chrondroitin sulfate/sodium hyaluronate composition |
US5143724A (en) | 1990-07-09 | 1992-09-01 | Biomatrix, Inc. | Biocompatible viscoelastic gel slurries, their preparation and use |
IT1270095B (en) * | 1994-09-28 | 1997-04-28 | Ibsa Inst Biochimique Sa | THERAPEUTIC COMPOSITIONS OF CHONDROITIN SULPHATE IN THE FORM OF ORAL ADMINISTRABLE GEL |
IT1291291B1 (en) | 1997-04-30 | 1999-01-07 | Chemedica Sa | USE OF HYALURONIC ACID AND RELATIVE SALTS FOR THE PREPARATION OF A WATER SOLUTION USEFUL AS INTRA-ARTICULAR WASHING LIQUID |
US5929050A (en) | 1998-02-27 | 1999-07-27 | Petito; George D. | Chondroitin sulfate composition and method for wound treatment |
JP2000072678A (en) * | 1998-06-15 | 2000-03-07 | Takeda Chem Ind Ltd | Chondropathy preventive and therapeutic agent |
WO2000044367A2 (en) | 1999-02-01 | 2000-08-03 | Dermal Research Laboratories, Inc. | A pharmaceutical composition of complex carbohydrates and essential oils and methods of using the same |
US6358917B1 (en) * | 1999-08-24 | 2002-03-19 | Jean D. A. Carruthers | Cosmetic use of botulinum toxin for treatment of downturned mouth |
US6924273B2 (en) * | 2000-10-03 | 2005-08-02 | Scott W. Pierce | Chondroprotective/restorative compositions and methods of use thereof |
ES2374157T3 (en) * | 2002-10-16 | 2012-02-14 | Arthrodynamic Technologies, Animal Health Division, Inc. | TREATMENT FOR TRAUMATIC SYNOVITIS AND DAMAGED ARTICULAR CARTRIDGE. |
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MXPA01011542A (en) | 2003-05-22 |
DK1443945T3 (en) | 2009-01-12 |
WO2003041724A1 (en) | 2003-05-22 |
CA2466499A1 (en) | 2003-05-22 |
EP1443945B1 (en) | 2008-10-22 |
US6906044B2 (en) | 2005-06-14 |
KR100989109B1 (en) | 2010-10-25 |
CN1585645A (en) | 2005-02-23 |
DE60229545D1 (en) | 2008-12-04 |
ES2312648T3 (en) | 2009-03-01 |
BR0214032A (en) | 2004-10-13 |
US20040214793A1 (en) | 2004-10-28 |
ZA200402717B (en) | 2005-02-23 |
MXPA04004452A (en) | 2004-08-11 |
HK1064043A1 (en) | 2005-01-21 |
EP1443945A1 (en) | 2004-08-11 |
JP2005515181A (en) | 2005-05-26 |
US6949525B2 (en) | 2005-09-27 |
KR20050044415A (en) | 2005-05-12 |
AU2002363589B2 (en) | 2007-11-29 |
US20040082540A1 (en) | 2004-04-29 |
US20050244358A1 (en) | 2005-11-03 |
JP4741183B2 (en) | 2011-08-03 |
JP2011105741A (en) | 2011-06-02 |
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