CA2478608A1 - Apparatus and methods for analyte measurement and immunoassay - Google Patents

Apparatus and methods for analyte measurement and immunoassay Download PDF

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Publication number
CA2478608A1
CA2478608A1 CA002478608A CA2478608A CA2478608A1 CA 2478608 A1 CA2478608 A1 CA 2478608A1 CA 002478608 A CA002478608 A CA 002478608A CA 2478608 A CA2478608 A CA 2478608A CA 2478608 A1 CA2478608 A1 CA 2478608A1
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Canada
Prior art keywords
cartridge
conduit
sample
sensor
analyte
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Granted
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CA002478608A
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French (fr)
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CA2478608C (en
Inventor
Graham Davis
Imants R. Lauks
Chao Lin
Cary James Miller
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Abbott Point of Care Inc
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Individual
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Priority to CA2737892A priority Critical patent/CA2737892C/en
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502723—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by venting arrangements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0663—Whole sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0421—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electrophoretic flow
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/046—Chemical or electrochemical formation of bubbles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0677—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
    • B01L2400/0683—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers mechanically breaking a wall or membrane within a channel or chamber
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G01N2035/0401—Sample carriers, cuvettes or reaction vessels
    • G01N2035/0429—Sample carriers adapted for special purposes
    • G01N2035/0436—Sample carriers adapted for special purposes with pre-packaged reagents, i.e. test-packs

Abstract

The present invention relates to an apparatus for conducting a variety of assays for the determination of analytes in liquid samples, and relates to the methods for such assays. In particular, the invention relates to a single-use cartridge designed to be adaptable to a variety of real-time assay protocols, preferably assays for the determination of analytes in biological samples using immunosensors or other ligand/ligand receptor-based biosensor embodiments. The cartridge provides novel features for processing a metered portion of a sample, for precise and flexible control of the movement of a sample or second fluid within the cartridge, for the amending of solutions with additional compounds during an assay, and for the construction of immunosensors capable of adaptation to diverse analyte measurements. The disclosed device and methods of use enjoy substantial benefits over the prior art, including simplicity of use by an operator, rapid in situ determinations of one or more analytes, and single-use methodology that minimizes the risk of contamination of both operator and patient. The disclosed invention is adaptable to the point-of-care clinical diagnostic field, including use in accident sites, emergency rooms, surgery, nursing homes, intensive care units, and non-medical environments.

Claims (62)

1. A cartridge for sensing at least one analyte in a sample, said cartridge comprising:
a sample holding chamber for receiving said sample and retaining said sample;
a first conduit connected to said sample holding chamber;
at least one analyte sensor, wherein said sensor comprises an analyte-responsive surface and said surface is within said first conduit;
a second conduit for retaining a fluid, said second conduit connected to said first conduit;
means for inserting at least one air segment into said first or second conduit; and pump means capable of displacing said sample from said holding chamber into said first conduit, said pump means further capable of displacing said fluid from said second conduit into said first conduit.
2. A cartridge for sensing at least one analyte in a sample, said cartridge comprising:
a sample holding chamber for receiving said sample and retaining said sample;
a first conduit connected to said sample holding chamber;
at least one analyte sensor, wherein said sensor comprises an analyte-responsive surface and said surface is within said first conduit;
a second conduit for retaining a fluid, said second conduit connected to said first conduit;
a valve connected to an opening in said first conduit, wherein said valve is closed by contact with said sample; and pump capable of displacing said sample from said holding chamber into said first conduit, said pump further capable of displacing said fluid from said second conduit into said first conduit.
3. A cartridge for sensing at least one analyte in a sample, said cartridge comprising:
a sample holding chamber for receiving said sample and retaining said sample;
a first conduit connected to said sample holding chamber;
at least one analyte sensor, wherein said sensor comprises an analyte-responsive surface and said surface is within said first conduit;
a second conduit for retaining a fluid, said second conduit connected to said first conduit;
a valve connected to an opening in said first conduit, wherein said valve is closed by contact with said sample;
means for inserting at least one air segment into said first or second conduit; and pump capable of displacing said sample from said holding chamber into said first conduit, said pump further capable of displacing said fluid from said second conduit into said first conduit.
4. The cartridge as in claim 1, 2, or 3, further comprising at least one sensor capable of detecting an air-liquid interface.
5. The cartridge as in claim 1, 2, or 3, wherein said cartridge is single-use.
6. The cartridge as in claim 1 or 3, wherein said segment is a single segment or a plurality of segments.
7. The cartridge as in claim 1 or 3, wherein said segment is inserted into said first conduit.
8. The cartridge as in claim 1 or 3, wherein said segment is inserted into said second conduit.
9. The cartridge as in claim 4 wherein said at least one sensor capable of detecting an air-liquid interface is a conductimetric sensor.
10. The cartridge as in claim 1, 2, or 3, wherein said cartridge further comprises a metering means for delivering a metered amount of said sample to said at least one analyte sensor.
11. The cartridge as in claim 10 wherein said metering means comprises a capillary stop within said first conduit.
12. The cartridge as in claim 1 or claim 3 wherein said means for inserting at least one air segment is selected from the group consisting of an air sac comprising a pneumatic means for displacing air from said air sac into said second conduit, a dry chemical that produces a gas when dissolved, a plurality of electrolysis electrodes operably connected to a current source, a porous means that absorbs fluid displacing air from said porous means into said conduit, and a vent that permits an air segment to enter the fluid when said pump means moves said fluid.
13. The cartridge as in claim 2 or claim 3 wherein said closeable valve is a dry sponge material coated with a fluid impermeable layer, a flap capable of blocking said valve and held open by a dry soluble compound or a gelling polymer.
14. The cartridge as in claim 1, 2, or 3, further comprising at least one constriction to control fluid flow within said first and second conduits.
15. The cartridge as in claim 1, 2, or 3 wherein said second conduit further comprises a valve responsive to hydrostatic pressure.
16. The cartridge as in claim 15, wherein said valve comprises a constriction in said conduit, wherein said constriction has a fluid-contacting surface comprising a hydrophobic surface.
17. The cartridge as in claim 1, 2, or 3, further comprising a third conduit connecting said second conduit and an overflow chamber.
18. The cartridge as in claim 1, 2, or 3, wherein said pump is selected from the group consisting of an air sac contacting a pneumatic means whereby pressure is applied to said air sac, a flexible diaphragm, a piston and cylinder, an electrodynamic pump, and a sonic pump.
19. The cartridge of claim 1, 2, or 3, in which said analyte-responsive surface comprises an antibody.
20. The cartridge as in claim 1, 2, or 3, wherein a portion of at least one conduit further comprise at least one dry reagent capable of dissolving in said fluid or sample.
21. The cartridge as in claim 20 wherein said at least one dry reagent is an antibody-enzyme conjugate, a substrate for said antibody-enzyme conjugate, or a blocking agent.
22. The cartridge as in claim 21 wherein said at least one antibody-enzyme conjugate comprises an enzyme that is urease, glucose oxidase, peroxidase, or alkaline phosphatase.
23. The cartridge as in claim 22 wherein said substrate is urea, glucose, hydrogen peroxide, or a molecule having a phosphate or peroxide moiety.
24. The cartridge as in claim 1, 2, or 3, wherein said at least one analyte sensor is an immunosensor.
25. The cartridge as in claim 1, 2, or 3 wherein said fluid comprises a substrate for an antibody-enzyme conjugate.
26. The cartridge as in claim 25 wherein said substrate is cleaved to produce an electroactive product.
27. The cartridge as in claim 26 wherein said substrate is a ferrocene or p-aminophenol phosphate.
28. The cartridge as in claim 1, 2, or 3, wherein said at least one analyte sensor is an amperometric sensor, a potentiometric sensor, or a reference sensor.
29. The cartridge as in claim 1, 2, or 3, further comprising a plurality of mechanical and electrical connections for insertion of said cartridge into a reading apparatus.
30. The cartridge as in claim 1, 2, or 3, wherein said at least one analyte sensor is formed on a substantially planar surface.
31. The cartridge as in claim 1, 2, or 3, further comprising a surface coating that decreases non-specific binding of a substance therein.
32. The cartridge as in claim 1, 2, or 3, further comprising an enzyme and a substrate capable of regenerating a product consumed by contact with said at least one analyte sensor, whereby a signal from said sensor is increased.
33. The cartridge as in claim 32 wherein said enzyme is glucose oxidase and said substrate is D-glucose.
34. The cartridge as in claim 1, 2, or 3, further comprising mobile microparticles capable interacting with said analyte and further comprising means for localizing said microparticles to said at least one sensor.
35. The cartridge as in claim 34, wherein said microparticles are magnetic, and said means for localizing said microparticles is a magnet field.
36. The cartridge as in claim 34, further comprising a filter element interposed between said sample holding chamber and said at least one analyze sensor, and adjacent said at least one sensor, whereby said microparticles are concentrated adjacent said at least one sensor.
37. An analyte sensor of claim 1, 2 or 3 comprising a microfabricated base sensor with a bioactive layer, superimposed over at least a portion of said base sensor, said layer comprising a sufficient amount of a biomolecules attached thereto, wherein said biomolecule is sensitive to said analyte.
38. The microfabricated sensor as in claim 37 in which said base sensor comprises an amperometric electrode.
39. The microfabricated sensor as in claim 37 further comprising a ground electrode.
40. The microfabricated sensor as in claim 37 further comprising a reference electrode.
41. The microfabricated sensor as in claim 37 wherein said biomolecule is an antibody or fragment thereof.
42. The microfabricated sensor as in claim 37 wherein said biomolecule is selected from the group consisting of ionophores, cofactors, polypeptides, proteins, glycopeptides, enzymes, immunoglobulins, antibodies, antigens, lectins, neurochemical receptors, oligonucleotides, polynucleotides, DNA, RNA, and mixtures thereof.
43. The microfabricated sensor as in claim 37 wherein said biomolecule is an antibody capable of binding an analyte selected from the group consisting of human chorionic gonadotrophin, troponin I, troponin T, troponin C, a troponin complex, creatine kinase, creatine kinase subunit M, creatine kinase subunit B, myoglobin, myosin light chain, fragments thereof, and modified fragments thereof.
44. The microfabricated sensor as in claim 43 wherein said modified fragments are generated by oxidation, reduction, deletion of at least one amino acid, addition of at least one amino acid, chemical modification with a natural moiety, or chemical modification with a synthetic moiety.
45. The microfabricated sensor as in claim 38, wherein said biomolecule has an affinity constant for said analyte ligand of greater than about 10 7 M-1.
46. A method for detecting at least one analyte in a sample using a sensing cartridge, said method comprising the steps of:
placing said sample into the sample holding chamber of the cartridge of claim 1 or claim 2;
activating said pump means, whereby said sample contacts said at least one analyte-responsive surface;
activating said pump means whereby said sample is displaced from contact with said analyte-responsive surface;

activating said pump means to contact said at least one analyte-responsive surface with said fluid; and recording a response from said at least one analyte sensor, whereby said analyte is detected.
47. A method for detecting at least one analyte in a sample using a sensing cartridge, said method comprising the steps of:
placing said sample into the sample holding chamber of the cartridge of claim 3;
activating said pump, whereby said sample contacts said at least one analyte-responsive surface;
activating said pump whereby said sample is displaced from contact with said analyte-responsive surface;
activating said pump to contact said at least one analyte-responsive surface with said fluid; and recording a response from said at least one analyte sensor, whereby said analyte is detected.
48. A cartridge for sensing at least one analyte in a sample, said cartridge comprising:
a sample holding chamber for receiving said sample and retaining said sample;
a first conduit connected to said sample holding chamber;
at least one analyte sensor, wherein said sensor comprises an analyte-responsive surface and said surface is within said first conduit;
a first and second vent within said first conduit wherein said sensor is positioned between said sample chamber and said vents;

a second conduit for retaining a fluid, said second conduit connected to said first conduit between said sample chamber and said vents;
pump means capable of displacing said sample from said holding chamber into said first conduit and further capable of closing said second vent to retain said sample in the region of said second vent; and wherein said pump means is further capable of displacing said fluid from said second conduit into said first conduit, and wherein said first vent permits at least one air segment to enter said fluid.
49. The cartridge of claim 48, wherein said pump means comprises a variable rate of displacement whereby the volume and number of air segments that enter the fluid is controlled.
50. The cartridge of claim 48, wherein the second conduit comprises a vent.
51. The cartridge of claim 48, wherein the sample contacts an adsorbent wicking material in proximity to second vent and is thereby retained and closes said vent.
52. The cartridge of claim 48, wherein the sample chamber further comprises a sample-metering element for providing a predetermined volume of sample to said first conduit.
53. The cartridge of claim 48, wherein said fluid in said second conduit is initially in a rupturable pouch.
54. The cartridge of claims 48 or claim 53, further comprising a reader device, said reader capable (a) of controlling and reading said analyte sensor, (b) of controlling said pump means, and (c) of controlling said rupturable pouch.
55. The cartridge of claim 48, wherein said first vent comprises a gas permeable membrane capable of controlling the rate of air entry through said first vent.
56. The cartridge of claim 1, 2, 3, or 48 wherein said sample holding chamber further comprises a closure means.
57. The cartridge of claim 48, wherein said analyte sensor is an electrochemical immunosensor comprising an electrode having a layer of immobilized first antibody that binds said analyte and further comprising a counter/reference electrode.
58. A method of measuring the amount of an analyte using an electrochemical assay in a conduit comprising a sensor; wherein said sensor comprises an electrode having a surface layer of immobilized antibody that binds said analyte, and a counter/reference electrode disposed within said conduit, said method comprising:
contacting said sensor with a liquid sample containing said analyte;
contacting said sensor with an enzyme-labeled antibody capable of binding said analyte, whereby a complex of immobilized antibody, analyte and labeled antibody is formed;
contacting said sensor with a solution comprising a substrate for said enzyme and at least one air segment to remove unbound analyte and labeled antibody from said sensor region;

removing substantially all said fluid from said sensor while retaining said fluid over said electrode, said counter/reference electrode, and a contiguous portion of the wall connecting said electrodes; and detecting the product of the reaction between said enzyme and said substrate using said sensor, whereby the amount of analyte in the liquid sample is measured.
59. The method of claim 58, wherein the sample comprises the enzyme-labeled antibody dissolved therein.
60. The method of claim 58, wherein the enzyme-labeled antibody is subsequently delivered in a liquid to the sensor that is not the sample, prior to delivery of said fluid.
61. The method of claim 58, wherein the layer retains a predetermined volume of fluid over the electrodes when the fluid is removed from the body of said conduit.
62. A method of claim 58, wherein at least a portion of the wall of the conduit is treated to reduce non-specific binding of the enzyme-labeled antibody.
CA2478608A 2002-03-05 2003-03-05 Apparatus and methods for analyte measurement and immunoassay Expired - Lifetime CA2478608C (en)

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Application Number Priority Date Filing Date Title
CA2737892A CA2737892C (en) 2002-03-05 2003-03-05 Apparatus and methods for analyte measurement and immunoassay

Applications Claiming Priority (3)

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US10/087,730 2002-03-05
US10/087,730 US7419821B2 (en) 2002-03-05 2002-03-05 Apparatus and methods for analyte measurement and immunoassay
PCT/US2003/006820 WO2003076937A2 (en) 2002-03-05 2003-03-05 Apparatus and methods for analyte measurement and immunoassay

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CA2478608C CA2478608C (en) 2011-06-28

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EP (1) EP1481246B1 (en)
JP (2) JP4347700B2 (en)
AT (1) ATE408143T1 (en)
AU (1) AU2003220041A1 (en)
CA (2) CA2737892C (en)
DE (1) DE60323466D1 (en)
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WO (1) WO2003076937A2 (en)

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