CA2481425A1 - Disposable sensor with enhanced sample port inlet - Google Patents
Disposable sensor with enhanced sample port inlet Download PDFInfo
- Publication number
- CA2481425A1 CA2481425A1 CA002481425A CA2481425A CA2481425A1 CA 2481425 A1 CA2481425 A1 CA 2481425A1 CA 002481425 A CA002481425 A CA 002481425A CA 2481425 A CA2481425 A CA 2481425A CA 2481425 A1 CA2481425 A1 CA 2481425A1
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- CA
- Canada
- Prior art keywords
- layer
- reagent
- strip
- electrode
- forming
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
- G01N27/3272—Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels
Abstract
A disposable biosensor for testing a fluid sample including a laminated stri p with a first and second end, a reference electrode embedded in the laminated strip proximate to the first end, at least one working electrode embedded in the laminated strip proximate to the first end and the reference electrode, an open path for receiving a fluid sample beginning from the first end and connecting to a vent spaced from the first end, the open path being sufficiently long to expose the reference electrode and the working electrod e to the fluid sample, and conductive contacts located at the second end of th e laminated strip. The laminated strip has a base layer with a conductive coating, a reagent holding layer, a channel forming layer and a cover having an inlet notch at the first end. The working electrode contains a reagent having an enzyme.
Claims (30)
1. A disposable biosensor comprising:
a laminated strip having a first strip end, a second strip end and a vent opening spaced from said first strip end, said laminated strip comprising a base layer with a conductive coating disposed thereon, said base layer having at least two electrodes delineated thereon, a reagent holding layer carried on said base layer, said reagent holding layer having at least two cutouts, a channel forming layer carried on said reagent holding layer, and a cover having a notch at said first strip end;
an enclosed channel between said first strip end and said vent opening, said enclosed channel containing said at least two cutouts;
a reagent disposed in said at least two cutouts forming a first working electrode and a reference electrode, said reagent containing an enzyme; and conductive contacts at said second strip end and insulated from said enclosed channel.
a laminated strip having a first strip end, a second strip end and a vent opening spaced from said first strip end, said laminated strip comprising a base layer with a conductive coating disposed thereon, said base layer having at least two electrodes delineated thereon, a reagent holding layer carried on said base layer, said reagent holding layer having at least two cutouts, a channel forming layer carried on said reagent holding layer, and a cover having a notch at said first strip end;
an enclosed channel between said first strip end and said vent opening, said enclosed channel containing said at least two cutouts;
a reagent disposed in said at least two cutouts forming a first working electrode and a reference electrode, said reagent containing an enzyme; and conductive contacts at said second strip end and insulated from said enclosed channel.
2. The biosensor of Claim 1 wherein said enzyme is selected from the group consisting of glucose oxidase, lactate oxidase, cholesterol oxidase, and creatinine amidohydrolase.
3. The biosensor of Claim 1 wherein said reagent holding layer has a third cutout having said reagent disposed therein and forming a second working electrode.
4. The biosensor of Claim 1 wherein said reagent further contains at least one of a redox mediator, a stabilizer, a binder, a surfactant, and a buffer.
5. The biosensor of Claim 4 wherein said stabilizer is a polyalkylene glycol, said binder is a cellulose material, and said surfactant is a polyoxyethylene ether.
6. The biosensor of Claim 5 wherein said stabilizer is polyethylene glycol, said binder is methyl cellulose, said surfactant is t-octylphenoxypolyethoxyethanol, and said buffer is a citrate buffer.
7. The biosensor of Claim 6 wherein said reagent is made from a mixture having starting components comprising about 1wt% to about 6.5wt% of said redox mediator, about 2.5wt% of said stabilizer, about 1wt% of said binder, and about .03wt% of said surfactant in said buffer.
8. The biosensor of Claim 7 wherein said citrate buffer is about 0.05M.
9. The biosensor of Claim 4 wherein said redox mediator is at least one of potassium ferricyanide and other inorganic and organic redox mediators.
10. The biosensor of Claim 1 wherein said conductive coating is gold or a gold and tin oxide mix.
11. The biosensor of Claim 1 wherein said base layer, said reagent holding layer, said channel forming layer, and said cover are made of a plastic dielectric material.
12. The biosensor of Claim 1 wherein said channel forming layer has a thickness sufficient to optimize the flow of said fluid sample along said open path.
13. The biosensor of Claim 7 wherein said reagent forming said reference electrode is made of a mixture having starting components comprising about 1wt% of said potassium ferricyanide, about 2.5wt% of said polyethylene glycol, about 1wt% of said methyl cellulose, about .03wt% of said t-octylphenoxypolyethoxyethanol, and said citrate buffer is about 0.05M.
14. The biosensor of Claim 9 wherein said reagent of said first working electrode is made of a mixture having starting components comprising about 6.5wt% of said potassium ferricyanide, about 2.5wt% of said polyethylene glycol, about 1wt% of said methyl cellulose, about .03wt% of said t-octylphenoxypolyethoxyethanol, and said pH buffer is about a 0.05M citrate buffer, and about 1wt% of said enzyme.
15. The biosensor of Claim 14 wherein said enzyme is glucose oxidase.
16. The biosensor of Claim 3 wherein the surface area of said first working electrode is substantially same as the surface area of said second working electrode.
17. The biosensor of Claim 3 wherein said reagent forming said second working electrode is substantially similar to said reagent forming said reference electrode.
18. A disposable electrode strip for detecting or measuring the concentration of at least one analyte in a fluid sample, said electrode strip comprising:
an insulating base layer having a first base end and a second base end;
a conductive layer disposed on one side of said base layer delineating at least three electrically-distinct conductive paths insulated from each other;
a reagent holding layer sized smaller than said base layer and overlaying a substantial portion of said conductive layer, said reagent holding layer having at least a first cutout portion and a second cutout portion spaced from said first base end, said first cutout portion exposing a limited area of a first of said at least three conductive paths and said second cutout portion exposing a limited area of a second and a third of said at least three conductive paths;
at least two electrode materials wherein a first electrode material is a reagent for measuring the concentration of said at least one analyte and wherein a second electrode material is a material suitable for use as a reference material, each of said at least two electrode materials contains at least a polyalkylene glycol as a stabilizer, said first material being disposed in said first cutout potion and said second material being disposed in said second cutout portion;
a channel forming layer sized to fit over and coextensive with said reagent holding layer, said channel forming layer having an opening configured to expose an area of said reagent holding layer a limited distance from said first base end, said area including said at least two cutout portions of said reagent holding layer; and a top layer sized to fit over and coextensive with said channel forming layer creating a sample fluid channel, said top layer having an inlet notch at a first top layer end, said first top layer end being coextensive with said first base end, and a top layer vent spaced from said first base end and configured to expose at least a small portion of said opening of said channel forming layer.
an insulating base layer having a first base end and a second base end;
a conductive layer disposed on one side of said base layer delineating at least three electrically-distinct conductive paths insulated from each other;
a reagent holding layer sized smaller than said base layer and overlaying a substantial portion of said conductive layer, said reagent holding layer having at least a first cutout portion and a second cutout portion spaced from said first base end, said first cutout portion exposing a limited area of a first of said at least three conductive paths and said second cutout portion exposing a limited area of a second and a third of said at least three conductive paths;
at least two electrode materials wherein a first electrode material is a reagent for measuring the concentration of said at least one analyte and wherein a second electrode material is a material suitable for use as a reference material, each of said at least two electrode materials contains at least a polyalkylene glycol as a stabilizer, said first material being disposed in said first cutout potion and said second material being disposed in said second cutout portion;
a channel forming layer sized to fit over and coextensive with said reagent holding layer, said channel forming layer having an opening configured to expose an area of said reagent holding layer a limited distance from said first base end, said area including said at least two cutout portions of said reagent holding layer; and a top layer sized to fit over and coextensive with said channel forming layer creating a sample fluid channel, said top layer having an inlet notch at a first top layer end, said first top layer end being coextensive with said first base end, and a top layer vent spaced from said first base end and configured to expose at least a small portion of said opening of said channel forming layer.
19. The strip of Claim 18 wherein said sample fluid channel is hydrophilic.
20. The device of Claim 18 wherein said first material and said second material further include a redox mediator, a binder, a surfactant, and a buffer.
21. The strip of Claim 20 wherein said redox mediator is at least one metal complex selected from the group consisting of ferrocene, ferrocene derivatives and potassium ferricyanide, said binder is a cellulose material, said surfactant is a polyoxyethylene ether, and said buffer has a pH of about to about 6.
22. The strip of Claim 21 wherein said mediator is potassium ferricyanide, said stabilizer is polyethylene glycol, said binder is methyl cellulose, said surfactant is t-octylphenoxypolyethoxyethanol, and said buffer is a citrate buffer.
23. The strip of Claim 22 wherein said first electrode material is made of a mixture having starting components comprising about 1wt% of said potassium ferricyanide, about 2.5wt% of said polyethylene glycol, about 1wt% of said methyl cellulose, and about 0.03wt% of said t-octylphenoxypolyethoxyethanol in said citrate buffer.
24. The strip of Claim 22 wherein said second electrode material is made of a mixture having starting components comprising about 6.5wt% of said potassium ferricyanide, about 2.5wt% of said polyethylene glycol, about 1wt% of said methyl cellulose, about 0.03wt% of said t-octylphenoxypolyethoxyethanol, and about 1wt% of an enzyme in said citrate buffer.
25. The strip of Claim 24 wherein said enzyme is glucose oxidase.
26. A method of making a disposable biosensor comprising:
scribing a conductive coating disposed on one side of an elongated base layer having an electrode end and an electrical contact end forming at least two elongated electrical conduits along the length of said base layer wherein a first conduit of said at least two electrical conduits has an L-shape wherein the L-shaped portion of said first conduit is adjacent said second conduit wherein said L-shaped end of said first conduit and a portion of said second conduit are located near said electrode end;
adhering a reagent holding layer over said base layer that is shorter than the length of said base layer such that a portion of each of said at least two elongated conduits is exposed at said electrical contact end, said reagent holding layer having at least two reagent holding cutouts spaced from said electrode end wherein a first cutout exposes a portion of said first conduit and a second cutout exposes a portion of said second conduit;
adding a reagent mixture to said first cutout forming a reference electrode and said second cutout forming a first working electrode, said reagent mixture in at least said first working electrode having an enzyme capable of catalyzing a reaction involving a substrate for the enzyme;
drying said reagent mixture forming a reagent matrix;
disposing a channel forming layer over said reagent holding layer, said channel forming layer having a U-shaped end portion defining a central elongated channel sized to expose said at least two reagent cutouts of said reagent holding layer; and disposing a top layer over said channel forming layer, said top layer having a vent opening spaced from said electrode end and a notch at said electrode end, said top layer forming an inlet and a capillary space with said U-shaped end portion wherein said vent exposes a portion of said central channel at the end of said capillary space opposite said inlet and said notch exposes a portion of said central channel at said inlet.
scribing a conductive coating disposed on one side of an elongated base layer having an electrode end and an electrical contact end forming at least two elongated electrical conduits along the length of said base layer wherein a first conduit of said at least two electrical conduits has an L-shape wherein the L-shaped portion of said first conduit is adjacent said second conduit wherein said L-shaped end of said first conduit and a portion of said second conduit are located near said electrode end;
adhering a reagent holding layer over said base layer that is shorter than the length of said base layer such that a portion of each of said at least two elongated conduits is exposed at said electrical contact end, said reagent holding layer having at least two reagent holding cutouts spaced from said electrode end wherein a first cutout exposes a portion of said first conduit and a second cutout exposes a portion of said second conduit;
adding a reagent mixture to said first cutout forming a reference electrode and said second cutout forming a first working electrode, said reagent mixture in at least said first working electrode having an enzyme capable of catalyzing a reaction involving a substrate for the enzyme;
drying said reagent mixture forming a reagent matrix;
disposing a channel forming layer over said reagent holding layer, said channel forming layer having a U-shaped end portion defining a central elongated channel sized to expose said at least two reagent cutouts of said reagent holding layer; and disposing a top layer over said channel forming layer, said top layer having a vent opening spaced from said electrode end and a notch at said electrode end, said top layer forming an inlet and a capillary space with said U-shaped end portion wherein said vent exposes a portion of said central channel at the end of said capillary space opposite said inlet and said notch exposes a portion of said central channel at said inlet.
27. The method of Claim 26 further comprising mixing a redox mediator, a stabilizer, a binder, a surfactant and a buffer forming said reagent mixture.
28. A method of making multiple, disposable sensors wherein each sensor has at least a first working electrode and a reference electrode, wherein said first working electrode contains an enzyme capable of catalyzing a reaction involving a substrate for the enzyme, said at least a first working electrode and said reference electrode being disposed in a fluid sample channel for measuring a fluid sample, said method comprising:
obtaining a base strip of an insulating material having a layer of conductive material disposed thereon, said base strip having a first edge and a second edge;
scribing in said conductive material a plurality of lines in a repetitive pattern wherein said plurality of lines contain a repetitive pattern forming three conductive paths in each of said repetitive pattern;
disposing a first middle layer of insulating material over said base strip, said first middle layer having a repetitive pattern of at least two cutouts wherein each cutout of each of said repetitive pattern exposes at least an electrode portion of said conductive layer wherein said repetitive pattern of said at least two cutouts are spaced from said first edge of said base strip, and wherein said first middle layer is sized to expose a contact portion of each of said three conductive paths of each repetitive pattern for a distance from said second edge of said base strip;
disposing a first reagent material on one of said at least two cutouts of each repetitive pattern and a second reagent material on the other of said at least two cutouts of each repetitive pattern;
drying said first reagent material and said second reagent material;
overlaying a second middle insulating layer over and coextensive with said first middle layer, said second middle layer having a plurality of elongated cutout portions in a repetitive pattern wherein each of said elongated cutout portions exposes a corresponding repetitive pattern of said at least two cutouts of said first middle layer;
disposing a top layer of insulating material over and coextensive with said second middle layer, said top layer having a plurality of vent openings and notch forming holes in a repetitive pattern wherein each of said vent openings exposes a portion of a corresponding repetitive pattern of said elongated cutout portion furthest from said first edge of said base strip and wherein each of said notch forming holes exposes a portion of said corresponding repetitive pattern of said elongated cutout portion closest to said first edge of said base strip, said base strip, said first middle layer, said second middle layer, and said top layer forming a laminated strip;
cutting along and parallel to said first edge of said laminated strip a predetermined distance creating a sample inlet port in each of said elongated cutout and an inlet notch in said top layer for each of said repetitive pattern;
cutting along and parallel to said second edge of said laminated strip a predetermined distance creating three separate contacts for each of said repetitive pattern; and separating each of said repetitive pattern forming one of each of said disposable sensors.
obtaining a base strip of an insulating material having a layer of conductive material disposed thereon, said base strip having a first edge and a second edge;
scribing in said conductive material a plurality of lines in a repetitive pattern wherein said plurality of lines contain a repetitive pattern forming three conductive paths in each of said repetitive pattern;
disposing a first middle layer of insulating material over said base strip, said first middle layer having a repetitive pattern of at least two cutouts wherein each cutout of each of said repetitive pattern exposes at least an electrode portion of said conductive layer wherein said repetitive pattern of said at least two cutouts are spaced from said first edge of said base strip, and wherein said first middle layer is sized to expose a contact portion of each of said three conductive paths of each repetitive pattern for a distance from said second edge of said base strip;
disposing a first reagent material on one of said at least two cutouts of each repetitive pattern and a second reagent material on the other of said at least two cutouts of each repetitive pattern;
drying said first reagent material and said second reagent material;
overlaying a second middle insulating layer over and coextensive with said first middle layer, said second middle layer having a plurality of elongated cutout portions in a repetitive pattern wherein each of said elongated cutout portions exposes a corresponding repetitive pattern of said at least two cutouts of said first middle layer;
disposing a top layer of insulating material over and coextensive with said second middle layer, said top layer having a plurality of vent openings and notch forming holes in a repetitive pattern wherein each of said vent openings exposes a portion of a corresponding repetitive pattern of said elongated cutout portion furthest from said first edge of said base strip and wherein each of said notch forming holes exposes a portion of said corresponding repetitive pattern of said elongated cutout portion closest to said first edge of said base strip, said base strip, said first middle layer, said second middle layer, and said top layer forming a laminated strip;
cutting along and parallel to said first edge of said laminated strip a predetermined distance creating a sample inlet port in each of said elongated cutout and an inlet notch in said top layer for each of said repetitive pattern;
cutting along and parallel to said second edge of said laminated strip a predetermined distance creating three separate contacts for each of said repetitive pattern; and separating each of said repetitive pattern forming one of each of said disposable sensors.
29. The method of Claim 28 further comprising drying said first reagent material and said second reagent material at a temperature and for a length of time sufficient to allow said first reagent material and said second reagent material to solidify and adhere to each of said electrode portion of each of said repetitive pattern of said three conductive paths.
30. The method of Claim 28 further comprising mixing a redox mediator, a stabilizer, a binder, a surfactant and a buffer forming said first reagent material, and mixing a redox mediator, a stabilizer, a binder, a surfactant, a buffer, and an enzyme forming said second reagent material.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10/126,819 US6837976B2 (en) | 2002-04-19 | 2002-04-19 | Disposable sensor with enhanced sample port inlet |
| US10/126,819 | 2002-04-19 | ||
| PCT/US2003/011554 WO2003089658A1 (en) | 2002-04-19 | 2003-04-16 | Disposable sensor with enhanced sample port inlet |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2481425A1 true CA2481425A1 (en) | 2003-10-30 |
| CA2481425C CA2481425C (en) | 2010-03-16 |
Family
ID=29248425
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2481425A Expired - Lifetime CA2481425C (en) | 2002-04-19 | 2003-04-16 | Disposable sensor with enhanced sample port inlet |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US6837976B2 (en) |
| EP (1) | EP1497449B1 (en) |
| JP (1) | JP4620356B2 (en) |
| AU (1) | AU2003228535A1 (en) |
| CA (1) | CA2481425C (en) |
| DE (1) | DE60319973T2 (en) |
| ES (1) | ES2302927T3 (en) |
| WO (1) | WO2003089658A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012006778A1 (en) * | 2010-07-14 | 2012-01-19 | 红电医学科技股份有限公司 | Test strip for detecting fluid |
Families Citing this family (102)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6036924A (en) | 1997-12-04 | 2000-03-14 | Hewlett-Packard Company | Cassette of lancet cartridges for sampling blood |
| US6391005B1 (en) | 1998-03-30 | 2002-05-21 | Agilent Technologies, Inc. | Apparatus and method for penetration with shaft having a sensor for sensing penetration depth |
| DE10057832C1 (en) * | 2000-11-21 | 2002-02-21 | Hartmann Paul Ag | Blood analysis device has syringe mounted in casing, annular mounting carrying needles mounted behind test strip and being swiveled so that needle can be pushed through strip and aperture in casing to take blood sample |
| US8641644B2 (en) | 2000-11-21 | 2014-02-04 | Sanofi-Aventis Deutschland Gmbh | Blood testing apparatus having a rotatable cartridge with multiple lancing elements and testing means |
| US9226699B2 (en) | 2002-04-19 | 2016-01-05 | Sanofi-Aventis Deutschland Gmbh | Body fluid sampling module with a continuous compression tissue interface surface |
| US7344507B2 (en) | 2002-04-19 | 2008-03-18 | Pelikan Technologies, Inc. | Method and apparatus for lancet actuation |
| EP1404232B1 (en) | 2001-06-12 | 2009-12-02 | Pelikan Technologies Inc. | Blood sampling apparatus and method |
| US7025774B2 (en) | 2001-06-12 | 2006-04-11 | Pelikan Technologies, Inc. | Tissue penetration device |
| JP4209767B2 (en) * | 2001-06-12 | 2009-01-14 | ペリカン テクノロジーズ インコーポレイテッド | Self-optimized cutting instrument with adaptive means for temporary changes in skin properties |
| US9795747B2 (en) | 2010-06-02 | 2017-10-24 | Sanofi-Aventis Deutschland Gmbh | Methods and apparatus for lancet actuation |
| US8337419B2 (en) | 2002-04-19 | 2012-12-25 | Sanofi-Aventis Deutschland Gmbh | Tissue penetration device |
| US7981056B2 (en) | 2002-04-19 | 2011-07-19 | Pelikan Technologies, Inc. | Methods and apparatus for lancet actuation |
| US20070100255A1 (en) * | 2002-04-19 | 2007-05-03 | Pelikan Technologies, Inc. | Method and apparatus for body fluid sampling and analyte sensing |
| US9427532B2 (en) | 2001-06-12 | 2016-08-30 | Sanofi-Aventis Deutschland Gmbh | Tissue penetration device |
| EP1404235A4 (en) * | 2001-06-12 | 2008-08-20 | Pelikan Technologies Inc | Method and apparatus for lancet launching device integrated onto a blood-sampling cartridge |
| ES2352998T3 (en) | 2001-06-12 | 2011-02-24 | Pelikan Technologies Inc. | LANCETA ELECTRIC ACTUATOR. |
| EP1404234B1 (en) | 2001-06-12 | 2011-02-09 | Pelikan Technologies Inc. | Apparatus for improving success rate of blood yield from a fingerstick |
| US7410468B2 (en) * | 2002-04-19 | 2008-08-12 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
| US7331931B2 (en) * | 2002-04-19 | 2008-02-19 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
| US9314194B2 (en) | 2002-04-19 | 2016-04-19 | Sanofi-Aventis Deutschland Gmbh | Tissue penetration device |
| US7491178B2 (en) * | 2002-04-19 | 2009-02-17 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
| US8267870B2 (en) | 2002-04-19 | 2012-09-18 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for body fluid sampling with hybrid actuation |
| US7229458B2 (en) | 2002-04-19 | 2007-06-12 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
| US7232451B2 (en) | 2002-04-19 | 2007-06-19 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
| US7717863B2 (en) * | 2002-04-19 | 2010-05-18 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
| US8579831B2 (en) | 2002-04-19 | 2013-11-12 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for penetrating tissue |
| US9795334B2 (en) * | 2002-04-19 | 2017-10-24 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for penetrating tissue |
| US7674232B2 (en) * | 2002-04-19 | 2010-03-09 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
| US7713214B2 (en) * | 2002-04-19 | 2010-05-11 | Pelikan Technologies, Inc. | Method and apparatus for a multi-use body fluid sampling device with optical analyte sensing |
| US8702624B2 (en) | 2006-09-29 | 2014-04-22 | Sanofi-Aventis Deutschland Gmbh | Analyte measurement device with a single shot actuator |
| US7524293B2 (en) * | 2002-04-19 | 2009-04-28 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
| US7371247B2 (en) | 2002-04-19 | 2008-05-13 | Pelikan Technologies, Inc | Method and apparatus for penetrating tissue |
| US7244265B2 (en) * | 2002-04-19 | 2007-07-17 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
| US7976476B2 (en) | 2002-04-19 | 2011-07-12 | Pelikan Technologies, Inc. | Device and method for variable speed lancet |
| US7901362B2 (en) | 2002-04-19 | 2011-03-08 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
| US7892185B2 (en) | 2002-04-19 | 2011-02-22 | Pelikan Technologies, Inc. | Method and apparatus for body fluid sampling and analyte sensing |
| US8784335B2 (en) * | 2002-04-19 | 2014-07-22 | Sanofi-Aventis Deutschland Gmbh | Body fluid sampling device with a capacitive sensor |
| US7291117B2 (en) | 2002-04-19 | 2007-11-06 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
| US9248267B2 (en) | 2002-04-19 | 2016-02-02 | Sanofi-Aventis Deustchland Gmbh | Tissue penetration device |
| US7648468B2 (en) * | 2002-04-19 | 2010-01-19 | Pelikon Technologies, Inc. | Method and apparatus for penetrating tissue |
| US7892183B2 (en) | 2002-04-19 | 2011-02-22 | Pelikan Technologies, Inc. | Method and apparatus for body fluid sampling and analyte sensing |
| US7547287B2 (en) * | 2002-04-19 | 2009-06-16 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
| US8360992B2 (en) | 2002-04-19 | 2013-01-29 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for penetrating tissue |
| US7297122B2 (en) | 2002-04-19 | 2007-11-20 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
| US8221334B2 (en) | 2002-04-19 | 2012-07-17 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for penetrating tissue |
| US7909778B2 (en) | 2002-04-19 | 2011-03-22 | Pelikan Technologies, Inc. | Method and apparatus for penetrating tissue |
| US20080044927A1 (en) * | 2002-10-30 | 2008-02-21 | Lien Ching H | Medical test strip |
| US20040087034A1 (en) * | 2002-10-30 | 2004-05-06 | Ching Ho Lien | Test strip |
| US7265881B2 (en) * | 2002-12-20 | 2007-09-04 | Hewlett-Packard Development Company, L.P. | Method and apparatus for measuring assembly and alignment errors in sensor assemblies |
| US8574895B2 (en) * | 2002-12-30 | 2013-11-05 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus using optical techniques to measure analyte levels |
| US7144485B2 (en) * | 2003-01-13 | 2006-12-05 | Hmd Biomedical Inc. | Strips for analyzing samples |
| WO2004107975A2 (en) | 2003-05-30 | 2004-12-16 | Pelikan Technologies, Inc. | Method and apparatus for fluid injection |
| DK1633235T3 (en) | 2003-06-06 | 2014-08-18 | Sanofi Aventis Deutschland | Apparatus for sampling body fluid and detecting analyte |
| WO2006001797A1 (en) | 2004-06-14 | 2006-01-05 | Pelikan Technologies, Inc. | Low pain penetrating |
| WO2004113917A2 (en) * | 2003-06-20 | 2004-12-29 | Roche Diagnostics Gmbh | Method and reagent for producing narrow, homogenous reagent strips |
| EP1671096A4 (en) | 2003-09-29 | 2009-09-16 | Pelikan Technologies Inc | Method and apparatus for an improved sample capture device |
| WO2005037095A1 (en) | 2003-10-14 | 2005-04-28 | Pelikan Technologies, Inc. | Method and apparatus for a variable user interface |
| DE602004025960D1 (en) * | 2003-10-31 | 2010-04-22 | Lifescan Scotland Ltd | MEASURING DEVICE FOR USE IN A METHOD OF REDUCING INTERFERENCE IN AN ELECTROCHEMICAL SENSOR USING TWO DIFFERENT APPROPRIATE POTENTIALS |
| JP4717636B2 (en) | 2003-12-04 | 2011-07-06 | パナソニック株式会社 | Method for measuring hematocrit (Hct), sensor used therefor, and measuring apparatus |
| WO2005054840A1 (en) | 2003-12-04 | 2005-06-16 | Matsushita Electric Industrial Co., Ltd. | Blood component measuring method, sensor used therefor, and measuring instrument |
| US7822454B1 (en) | 2005-01-03 | 2010-10-26 | Pelikan Technologies, Inc. | Fluid sampling device with improved analyte detecting member configuration |
| US8668656B2 (en) | 2003-12-31 | 2014-03-11 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for improving fluidic flow and sample capture |
| JP4689601B2 (en) * | 2004-04-19 | 2011-05-25 | パナソニック株式会社 | Blood component measuring method, biosensor and measuring apparatus used therefor |
| EP1751546A2 (en) | 2004-05-20 | 2007-02-14 | Albatros Technologies GmbH & Co. KG | Printable hydrogel for biosensors |
| US7118667B2 (en) * | 2004-06-02 | 2006-10-10 | Jin Po Lee | Biosensors having improved sample application and uses thereof |
| US9775553B2 (en) * | 2004-06-03 | 2017-10-03 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for a fluid sampling device |
| US9820684B2 (en) | 2004-06-03 | 2017-11-21 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for a fluid sampling device |
| US8652831B2 (en) | 2004-12-30 | 2014-02-18 | Sanofi-Aventis Deutschland Gmbh | Method and apparatus for analyte measurement test time |
| CA2614299C (en) | 2005-06-13 | 2014-06-03 | Nippon Kayaku Kabushiki Kaisha | Method of assaying blood component by using whole blood and measurement kit |
| US7611621B2 (en) * | 2005-06-13 | 2009-11-03 | Nova Biomedical Corporation | Disposable oxygen sensor and method for correcting oxygen effect on oxidase-based analytical devices |
| JP4501793B2 (en) * | 2005-06-24 | 2010-07-14 | パナソニック株式会社 | Biosensor |
| US8617366B2 (en) * | 2005-12-12 | 2013-12-31 | Nova Biomedical Corporation | Disposable urea sensor and system for determining creatinine and urea nitrogen-to-creatinine ratio in a single device |
| US20080006530A1 (en) * | 2006-06-19 | 2008-01-10 | Handani Winarta | Capillary Flow Control in a Flow Channel |
| WO2008040998A2 (en) * | 2006-10-05 | 2008-04-10 | Lifescan Scotland Limited | Systems and methods for determining a substantially hematocrit independent analyte concentration |
| US9046480B2 (en) | 2006-10-05 | 2015-06-02 | Lifescan Scotland Limited | Method for determining hematocrit corrected analyte concentrations |
| ATE550642T1 (en) * | 2006-12-14 | 2012-04-15 | Nippon Kayaku Kk | METHOD FOR MEASURING 1,5-ANHYDROGLUCITOL IN WHOLE BLOOD |
| US7802467B2 (en) * | 2006-12-22 | 2010-09-28 | Abbott Diabetes Care Inc. | Analyte sensors and methods of use |
| US8299317B2 (en) * | 2007-03-29 | 2012-10-30 | Kimberly-Clark Worldwide, Inc. | Absorbent articles with external access to internal conductors |
| TW200914826A (en) * | 2007-09-21 | 2009-04-01 | Apex Biotechnology Corp | Electrochemical quantitative analysis system and method for the same |
| KR100890988B1 (en) * | 2007-10-29 | 2009-03-31 | 주식회사 아이센스 | Electrochemical biosensor equipped with sampling port which enables uniform introduction of a small amount of sample |
| US8603768B2 (en) * | 2008-01-17 | 2013-12-10 | Lifescan, Inc. | System and method for measuring an analyte in a sample |
| EP2265324B1 (en) | 2008-04-11 | 2015-01-28 | Sanofi-Aventis Deutschland GmbH | Integrated analyte measurement system |
| US20100187132A1 (en) * | 2008-12-29 | 2010-07-29 | Don Alden | Determination of the real electrochemical surface areas of screen printed electrodes |
| US9375169B2 (en) | 2009-01-30 | 2016-06-28 | Sanofi-Aventis Deutschland Gmbh | Cam drive for managing disposable penetrating member actions with a single motor and motor and control system |
| KR100918027B1 (en) * | 2009-02-19 | 2009-09-18 | 주식회사 올메디쿠스 | Bio-sensor provided with code electrode, method for manufacturing the same, and method for taking sensor information from the same |
| US8500990B2 (en) * | 2009-04-22 | 2013-08-06 | Nova Biomedical Corporation | Electrochemical biosensors based on NAD(P)-dependent dehydrogenase enzymes |
| US8965476B2 (en) | 2010-04-16 | 2015-02-24 | Sanofi-Aventis Deutschland Gmbh | Tissue penetration device |
| US8603309B2 (en) | 2011-09-12 | 2013-12-10 | Nova Biomedical Corporation | Disposable sensor for electrochemical detection of hemoglobin |
| US20130084590A1 (en) * | 2011-09-30 | 2013-04-04 | Lifescan Scotland Ltd. | Analytical test strip with bodily fluid phase-shift measurement electrodes |
| US20130098775A1 (en) * | 2011-10-20 | 2013-04-25 | Nova Biomedical Corporation | Glucose biosensor with improved shelf life |
| KR101466222B1 (en) * | 2012-06-01 | 2014-12-01 | 주식회사 아이센스 | Electrochemical biosensor with improved accuracy |
| TW201415015A (en) * | 2012-10-15 | 2014-04-16 | Ichia Tech Inc | Method for manufacturing test plate for biologic liquid and structure for the same |
| CN103091377B (en) * | 2013-02-05 | 2015-01-21 | 三诺生物传感股份有限公司 | Biosensor |
| US10898116B2 (en) * | 2013-03-15 | 2021-01-26 | Cambridge Medical Technologies LLC | Methods of manufacture to optimize performance of transdermal sampling and analysis device |
| EP2781919A1 (en) | 2013-03-19 | 2014-09-24 | Roche Diagniostics GmbH | Method / device for generating a corrected value of an analyte concentration in a sample of a body fluid |
| CN104062319A (en) * | 2013-03-22 | 2014-09-24 | 毅嘉科技股份有限公司 | Manufacturing method of test sheet for biological liquid and structure of test sheet |
| CN104007150A (en) * | 2013-12-04 | 2014-08-27 | 西南大学 | Conductive polymer-based all-print biological and environmental sensor and making method thereof |
| CN104450864A (en) * | 2014-12-18 | 2015-03-25 | 三诺生物传感股份有限公司 | Composition and application thereof |
| US9891209B2 (en) * | 2015-05-29 | 2018-02-13 | C A Casyso Gmbh | Electrode assembly for measurement of platelet function in whole blood |
| US10802071B2 (en) * | 2017-12-01 | 2020-10-13 | International Business Machines Corporation | Elemental mercury-containing probe card |
| US11633129B2 (en) | 2019-04-05 | 2023-04-25 | Cambridge Medical Technologies LLC | Non-invasive transdermal sampling and analysis device incorporating redox cofactors |
| US11375931B2 (en) | 2019-08-08 | 2022-07-05 | Cambridge Medical Technologies LLC | Non-invasive transdermal sampling and analysis device incorporating an electrochemical bioassay |
Family Cites Families (60)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CH559912A5 (en) | 1971-09-09 | 1975-03-14 | Hoffmann La Roche | |
| US3979274A (en) | 1975-09-24 | 1976-09-07 | The Yellow Springs Instrument Company, Inc. | Membrane for enzyme electrodes |
| US4137495A (en) | 1976-03-27 | 1979-01-30 | Brown David M B | Oil detector |
| US4053381A (en) | 1976-05-19 | 1977-10-11 | Eastman Kodak Company | Device for determining ionic activity of components of liquid drops |
| FR2387659A1 (en) | 1977-04-21 | 1978-11-17 | Armines | GLYCEMIA CONTROL AND REGULATION DEVICE |
| US4133735A (en) | 1977-09-27 | 1979-01-09 | The Board Of Regents Of The University Of Washington | Ion-sensitive electrode and processes for making the same |
| JPS5912135B2 (en) | 1977-09-28 | 1984-03-21 | 松下電器産業株式会社 | enzyme electrode |
| US4321123A (en) | 1978-04-21 | 1982-03-23 | Matsushita Electric Industrial Co., Ltd. | Coenzyme immobilized electrode |
| US4185131A (en) | 1978-06-28 | 1980-01-22 | United Technologies Corporation | Screen printing method for making an electrochemical cell electrode |
| US4184936A (en) | 1978-07-24 | 1980-01-22 | Eastman Kodak Company | Device for determining ionic activity |
| US4233029A (en) | 1978-10-25 | 1980-11-11 | Eastman Kodak Company | Liquid transport device and method |
| US4225410A (en) | 1978-12-04 | 1980-09-30 | Technicon Instruments Corporation | Integrated array of electrochemical sensors |
| US4273639A (en) | 1979-06-20 | 1981-06-16 | Eastman Kodak Company | Capillary bridge in apparatus for determining ionic activity |
| US4310399A (en) | 1979-07-23 | 1982-01-12 | Eastman Kodak Company | Liquid transport device containing means for delaying capillary flow |
| US4303887A (en) | 1979-10-29 | 1981-12-01 | United States Surgical Corporation | Electrical liquid conductivity measuring system |
| US4301414A (en) | 1979-10-29 | 1981-11-17 | United States Surgical Corporation | Disposable sample card and method of making same |
| US4413407A (en) | 1980-03-10 | 1983-11-08 | Eastman Kodak Company | Method for forming an electrode-containing device with capillary transport between electrodes |
| US4356074A (en) | 1980-08-25 | 1982-10-26 | The Yellow Springs Instrument Company, Inc. | Substrate specific galactose oxidase enzyme electrodes |
| GB2096825A (en) | 1981-04-09 | 1982-10-20 | Sibbald Alastair | Chemical sensitive semiconductor field effect transducer |
| FR2508305B1 (en) | 1981-06-25 | 1986-04-11 | Slama Gerard | DEVICE FOR CAUSING A LITTLE BITE TO COLLECT A BLOOD DROP |
| DE3278334D1 (en) | 1981-10-23 | 1988-05-19 | Genetics Int Inc | Sensor for components of a liquid mixture |
| US4418148A (en) | 1981-11-05 | 1983-11-29 | Miles Laboratories, Inc. | Multilayer enzyme electrode membrane |
| US4473457A (en) | 1982-03-29 | 1984-09-25 | Eastman Kodak Company | Liquid transport device providing diversion of capillary flow into a non-vented second zone |
| EP0096095B1 (en) | 1982-06-14 | 1988-11-09 | Corporation Ohmicron | Semiconductor device, sensor and method for determining the concentration of an analyte in a medium |
| US4454007A (en) | 1983-01-27 | 1984-06-12 | E. I. Du Pont De Nemours And Company | Ion-selective layered sensor and methods of making and using the same |
| US4490216A (en) | 1983-02-03 | 1984-12-25 | Molecular Devices Corporation | Lipid membrane electroanalytical elements and method of analysis therewith |
| WO1984003562A1 (en) | 1983-03-11 | 1984-09-13 | Matsushita Electric Ind Co Ltd | Biosensor |
| GB8308389D0 (en) | 1983-03-26 | 1983-05-05 | Cambridge Life Sciences | Assay technique |
| US5682884A (en) | 1983-05-05 | 1997-11-04 | Medisense, Inc. | Strip electrode with screen printing |
| US5509410A (en) | 1983-06-06 | 1996-04-23 | Medisense, Inc. | Strip electrode including screen printing of a single layer |
| US4591550A (en) | 1984-03-01 | 1986-05-27 | Molecular Devices Corporation | Device having photoresponsive electrode for determining analytes including ligands and antibodies |
| US4654127A (en) | 1984-04-11 | 1987-03-31 | Sentech Medical Corporation | Self-calibrating single-use sensing device for clinical chemistry and method of use |
| AU581669B2 (en) | 1984-06-13 | 1989-03-02 | Applied Research Systems Ars Holding N.V. | Photometric instruments, their use in methods of optical analysis, and ancillary devices therefor |
| US5141868A (en) | 1984-06-13 | 1992-08-25 | Internationale Octrooi Maatschappij "Octropa" Bv | Device for use in chemical test procedures |
| US5185256A (en) | 1985-06-21 | 1993-02-09 | Matsushita Electric Industrial Co., Ltd. | Method for making a biosensor |
| WO1986007632A1 (en) | 1985-06-21 | 1986-12-31 | Matsushita Electric Industrial Co., Ltd. | Biosensor and method of manufacturing same |
| GB8618022D0 (en) | 1986-07-23 | 1986-08-28 | Unilever Plc | Electrochemical measurements |
| GB8626081D0 (en) | 1986-10-31 | 1986-12-03 | Unilever Plc | Printing processes |
| US4900405A (en) | 1987-07-15 | 1990-02-13 | Sri International | Surface type microelectronic gas and vapor sensor |
| DE68924026T3 (en) | 1988-03-31 | 2008-01-10 | Matsushita Electric Industrial Co., Ltd., Kadoma | BIOSENSOR AND ITS MANUFACTURE. |
| AU634863B2 (en) | 1989-12-15 | 1993-03-04 | Roche Diagnostics Operations Inc. | Redox mediator reagent and biosensor |
| US5508171A (en) | 1989-12-15 | 1996-04-16 | Boehringer Mannheim Corporation | Assay method with enzyme electrode system |
| JPH0820412B2 (en) | 1990-07-20 | 1996-03-04 | 松下電器産業株式会社 | Quantitative analysis method and device using disposable sensor |
| JP3118015B2 (en) | 1991-05-17 | 2000-12-18 | アークレイ株式会社 | Biosensor and separation and quantification method using the same |
| US5264103A (en) | 1991-10-18 | 1993-11-23 | Matsushita Electric Industrial Co., Ltd. | Biosensor and a method for measuring a concentration of a substrate in a sample |
| JP3135959B2 (en) | 1991-12-12 | 2001-02-19 | アークレイ株式会社 | Biosensor and separation and quantification method using the same |
| FR2701117B1 (en) | 1993-02-04 | 1995-03-10 | Asulab Sa | Electrochemical measurement system with multizone sensor, and its application to glucose measurement. |
| US5762770A (en) | 1994-02-21 | 1998-06-09 | Boehringer Mannheim Corporation | Electrochemical biosensor test strip |
| US5437999A (en) | 1994-02-22 | 1995-08-01 | Boehringer Mannheim Corporation | Electrochemical sensor |
| US5628890A (en) | 1995-09-27 | 1997-05-13 | Medisense, Inc. | Electrochemical sensor |
| AUPN661995A0 (en) * | 1995-11-16 | 1995-12-07 | Memtec America Corporation | Electrochemical cell 2 |
| US5755953A (en) | 1995-12-18 | 1998-05-26 | Abbott Laboratories | Interference free biosensor |
| US5708247A (en) | 1996-02-14 | 1998-01-13 | Selfcare, Inc. | Disposable glucose test strips, and methods and compositions for making same |
| US5759364A (en) | 1997-05-02 | 1998-06-02 | Bayer Corporation | Electrochemical biosensor |
| US5997817A (en) * | 1997-12-05 | 1999-12-07 | Roche Diagnostics Corporation | Electrochemical biosensor test strip |
| US6258229B1 (en) * | 1999-06-02 | 2001-07-10 | Handani Winarta | Disposable sub-microliter volume sensor and method of making |
| US6287451B1 (en) | 1999-06-02 | 2001-09-11 | Handani Winarta | Disposable sensor and method of making |
| US6645359B1 (en) * | 2000-10-06 | 2003-11-11 | Roche Diagnostics Corporation | Biosensor |
| KR100445489B1 (en) * | 1999-11-15 | 2004-08-21 | 마츠시타 덴끼 산교 가부시키가이샤 | Biosensor, method of forming thin-film electrode, and method and apparatus for quantitative determination |
| US6767441B1 (en) * | 2001-07-31 | 2004-07-27 | Nova Biomedical Corporation | Biosensor with peroxidase enzyme |
-
2002
- 2002-04-19 US US10/126,819 patent/US6837976B2/en not_active Expired - Lifetime
-
2003
- 2003-04-16 EP EP03726290A patent/EP1497449B1/en not_active Expired - Lifetime
- 2003-04-16 AU AU2003228535A patent/AU2003228535A1/en not_active Abandoned
- 2003-04-16 JP JP2003586367A patent/JP4620356B2/en not_active Expired - Lifetime
- 2003-04-16 WO PCT/US2003/011554 patent/WO2003089658A1/en active Application Filing
- 2003-04-16 CA CA2481425A patent/CA2481425C/en not_active Expired - Lifetime
- 2003-04-16 ES ES03726290T patent/ES2302927T3/en not_active Expired - Lifetime
- 2003-04-16 DE DE60319973T patent/DE60319973T2/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012006778A1 (en) * | 2010-07-14 | 2012-01-19 | 红电医学科技股份有限公司 | Test strip for detecting fluid |
Also Published As
| Publication number | Publication date |
|---|---|
| EP1497449B1 (en) | 2008-03-26 |
| US20040224369A1 (en) | 2004-11-11 |
| EP1497449A1 (en) | 2005-01-19 |
| CA2481425C (en) | 2010-03-16 |
| AU2003228535A1 (en) | 2003-11-03 |
| ES2302927T3 (en) | 2008-08-01 |
| US6837976B2 (en) | 2005-01-04 |
| JP4620356B2 (en) | 2011-01-26 |
| DE60319973D1 (en) | 2008-05-08 |
| DE60319973T2 (en) | 2009-04-16 |
| JP2005523443A (en) | 2005-08-04 |
| WO2003089658A1 (en) | 2003-10-30 |
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