CA2521171A1 - Microfluidic devices and methods of using same - Google Patents
Microfluidic devices and methods of using same Download PDFInfo
- Publication number
- CA2521171A1 CA2521171A1 CA002521171A CA2521171A CA2521171A1 CA 2521171 A1 CA2521171 A1 CA 2521171A1 CA 002521171 A CA002521171 A CA 002521171A CA 2521171 A CA2521171 A CA 2521171A CA 2521171 A1 CA2521171 A1 CA 2521171A1
- Authority
- CA
- Canada
- Prior art keywords
- sample fluid
- channel
- valve
- microfluidic device
- actuated
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract 11
- 238000006243 chemical reaction Methods 0.000 claims abstract 8
- 108020004707 nucleic acids Proteins 0.000 claims abstract 2
- 150000007523 nucleic acids Chemical class 0.000 claims abstract 2
- 102000039446 nucleic acids Human genes 0.000 claims abstract 2
- 239000012530 fluid Substances 0.000 claims 24
- 238000003752 polymerase chain reaction Methods 0.000 claims 4
- 238000002955 isolation Methods 0.000 claims 2
- 238000007847 digital PCR Methods 0.000 claims 1
- 238000004458 analytical method Methods 0.000 abstract 2
- 239000003153 chemical reaction reagent Substances 0.000 abstract 2
- 238000004519 manufacturing process Methods 0.000 abstract 2
- 230000003321 amplification Effects 0.000 abstract 1
- 238000003491 array Methods 0.000 abstract 1
- 238000010195 expression analysis Methods 0.000 abstract 1
- 230000014509 gene expression Effects 0.000 abstract 1
- 238000003205 genotyping method Methods 0.000 abstract 1
- 238000010438 heat treatment Methods 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502723—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by venting arrangements
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0642—Filling fluids into wells by specific techniques
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
- B01L2200/142—Preventing evaporation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
- B01L2200/143—Quality control, feedback systems
- B01L2200/147—Employing temperature sensors
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/12—Specific details about materials
- B01L2300/123—Flexible; Elastomeric
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/06—Valves, specific forms thereof
- B01L2400/0633—Valves, specific forms thereof with moving parts
- B01L2400/0638—Valves, specific forms thereof with moving parts membrane valves, flap valves
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Abstract
A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.
Claims (13)
1. A microfluidic device comprising:
a fluidic channel having a first end and a second end in fluid communication with each other through said channel;
an inlet in communication with said channel, said inlet for introducing a sample fluid;
and, a valve, said valve, when actuated, causing said first channel end to become fluidically separated from said second channel end;
wherein when said sample fluid is introduced into said fluidic channel, and said valve is actuated, said sample fluid is divided into separate sample fluid portions.
a fluidic channel having a first end and a second end in fluid communication with each other through said channel;
an inlet in communication with said channel, said inlet for introducing a sample fluid;
and, a valve, said valve, when actuated, causing said first channel end to become fluidically separated from said second channel end;
wherein when said sample fluid is introduced into said fluidic channel, and said valve is actuated, said sample fluid is divided into separate sample fluid portions.
2. The microfluidic device of claim 1, further comprising an outlet in communication with said channel, said outlet for removing some or all of said sample fluid.
3. The microfluidic device of claim 2, further comprising a second valve, said second valve, when actuated, causing said outlet and said fluidic channel to be in fluidic isolation.
4. The microfluidic device of claim 1, wherein said sample fluid is introduced into said fluidic channel by blind filling.
5. The microfluidic device of claim 3, wherein said sample fluid is introduced into said fluidic channel by blind filling while said second valve is actuated.
6. The microfluidic device of claim 3, wherein said second valve, when de-actuated, permits removal of some or all of said sample fluid from said fluidic channel.
7. A method of analyzing a sample fluid for a reaction to a condition comprising the steps of:
(i) providing a microfluidic device, said microfluidic device comprising:
a fluidic channel having a first end and a second end in fluid communication with each other through said channel;
an inlet in communication with said channel, said inlet for introducing said sample fluid; and, a valve, said valve, when actuated, causing said first channel end to become fluidically separated from said second channel end;
wherein when said sample fluid is introduced into said fluidic channel, and said valve is actuated, said sample fluid is divided into separate sample fluid portions;
(ii) introducing said sample fluid into said inlet, said sample fluid filling said first and second channel ends;
(iii) actuating said valve to separate said sample fluid into said separate portions within said first and second channel ends;
(iv) exposing said sample fluid to said conditions;
(v) analyzing said sample fluid to determine whether said conditions produced said reaction.
(i) providing a microfluidic device, said microfluidic device comprising:
a fluidic channel having a first end and a second end in fluid communication with each other through said channel;
an inlet in communication with said channel, said inlet for introducing said sample fluid; and, a valve, said valve, when actuated, causing said first channel end to become fluidically separated from said second channel end;
wherein when said sample fluid is introduced into said fluidic channel, and said valve is actuated, said sample fluid is divided into separate sample fluid portions;
(ii) introducing said sample fluid into said inlet, said sample fluid filling said first and second channel ends;
(iii) actuating said valve to separate said sample fluid into said separate portions within said first and second channel ends;
(iv) exposing said sample fluid to said conditions;
(v) analyzing said sample fluid to determine whether said conditions produced said reaction.
8. The method of claim 7, wherein said microfluidic device further comprises an outlet in communication with said channel, said outlet for removing some or all of said sample fluid, and said method further comprises a step for removing said sample fluid from said fluidic channel after said exposing step.
9. The method of claim 8, wherein said microfluidic device further comprises a second valve, said second valve, when actuated, causing said outlet and said fluidic channel to be in fluidic isolation, and said method further comprises actuating said valve prior to said introducing step to blind fill said sample fluid into said fluidic channel.
10. The method of claim 7, wherein said sample fluid is introduced into said fluidic channel by blind filling.
11. The method of claim 7, wherein said sample further comprises an amplifiable nucleic acid target and said conditions are polymerase chain reaction (PCR) conditions, and said reaction results in a PCR product being formed.
12. The method of claim 7, wherein said PCR is digital PCR.
13. The method of claim 11, wherein said reaction produces two different color results.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA2807564A CA2807564C (en) | 2003-04-03 | 2004-04-05 | Microfluidic devices and methods of using same |
CA2807591A CA2807591C (en) | 2003-04-03 | 2004-04-05 | Microfluidic devices and methods of using same |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US46063403P | 2003-04-03 | 2003-04-03 | |
US60/460,634 | 2003-04-03 | ||
PCT/US2004/010492 WO2004089810A2 (en) | 2003-04-03 | 2004-04-05 | Microfluidic devices and methods of using same |
Related Child Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2807564A Division CA2807564C (en) | 2003-04-03 | 2004-04-05 | Microfluidic devices and methods of using same |
CA2807591A Division CA2807591C (en) | 2003-04-03 | 2004-04-05 | Microfluidic devices and methods of using same |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2521171A1 true CA2521171A1 (en) | 2004-10-21 |
CA2521171C CA2521171C (en) | 2013-05-28 |
Family
ID=33159790
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2521171A Expired - Lifetime CA2521171C (en) | 2003-04-03 | 2004-04-05 | Microfluidic devices and methods of using same |
Country Status (6)
Country | Link |
---|---|
US (2) | US7666361B2 (en) |
EP (2) | EP2340890B1 (en) |
JP (4) | JP5419248B2 (en) |
AU (1) | AU2004228678A1 (en) |
CA (1) | CA2521171C (en) |
WO (1) | WO2004089810A2 (en) |
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WO2004089810A3 (en) | 2005-07-07 |
US7666361B2 (en) | 2010-02-23 |
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EP2340890A1 (en) | 2011-07-06 |
US20050129581A1 (en) | 2005-06-16 |
EP1615721B1 (en) | 2014-06-18 |
CA2521171C (en) | 2013-05-28 |
AU2004228678A1 (en) | 2004-10-21 |
JP2016028257A (en) | 2016-02-25 |
JP5419248B2 (en) | 2014-02-19 |
EP1615721A4 (en) | 2006-08-02 |
EP2340890B1 (en) | 2016-10-19 |
US20080230387A1 (en) | 2008-09-25 |
JP2011097955A (en) | 2011-05-19 |
JP2014110815A (en) | 2014-06-19 |
WO2004089810A2 (en) | 2004-10-21 |
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