CA2538966A1 - Electrochemical cell - Google Patents

Electrochemical cell Download PDF

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Publication number
CA2538966A1
CA2538966A1 CA002538966A CA2538966A CA2538966A1 CA 2538966 A1 CA2538966 A1 CA 2538966A1 CA 002538966 A CA002538966 A CA 002538966A CA 2538966 A CA2538966 A CA 2538966A CA 2538966 A1 CA2538966 A1 CA 2538966A1
Authority
CA
Canada
Prior art keywords
working electrode
electrode
counter
current
electrochemical cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002538966A
Other languages
French (fr)
Other versions
CA2538966C (en
Inventor
Alastair Mcindoe Hodges
Thomas William Beck
Oddvar Johansen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
LifeScan Inc
Original Assignee
Lifescan, Inc.
Alastair Mcindoe Hodges
Thomas William Beck
Oddvar Johansen
Memtec Limited
Memtec America Corporation
Usf Filtration And Separations Group Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lifescan, Inc., Alastair Mcindoe Hodges, Thomas William Beck, Oddvar Johansen, Memtec Limited, Memtec America Corporation, Usf Filtration And Separations Group Inc. filed Critical Lifescan, Inc.
Priority to CA002573423A priority Critical patent/CA2573423C/en
Publication of CA2538966A1 publication Critical patent/CA2538966A1/en
Application granted granted Critical
Publication of CA2538966C publication Critical patent/CA2538966C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/004—Enzyme electrodes mediator-assisted
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • C12Q1/006—Enzyme electrodes involving specific analytes or enzymes for glucose
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
    • G01N27/3273—Devices therefor, e.g. test element readers, circuitry

Abstract

A method for determining the concentration of a reduced (or oxidised) form of a redox species in an electrochemical cell of the kind comprising a working electrode and a counter electrode spaced from the working electrode by a predetermined distance, said method comprising the steps of (1) applying an electric potential difference between the electrodes; (2) selecting the potential of the working electrode such that the rate of electro-oxidation of the reduced form (or electro-reduction of the oxidised form) of the species is diffusion controlled, (3) selecting the spacing between the working electrode and the counter electrode so that reaction products from the counter electrode arrive at the working electrode; (4) determining current as a function of time after application of the potential and prior to achievement of a steady state; (5) estimating the magnitude of the steady state current, and'(6) obtaining from the change in current with time and the magnitude of the steady state current, a value indicative of the diffusion coefficient and/or of the concentration of the reduced form (or the oxidised form) of the species. Also disclosed is an apparatus for determining the concentration of a redox species in an electrochemical cell comprising. an electrochemical cell having a working electrode and a counter (or counter/reference) electrode, means for applying and electric potential difference between said electrodes, means for measuring the change in current with time, and characterised in that the working electrode is spaced from the counter electrode by less than 500 µm.
CA002538966A 1995-06-19 1996-06-19 Electrochemical cell Expired - Lifetime CA2538966C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA002573423A CA2573423C (en) 1995-06-19 1996-06-19 Electrochemical cell

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AUPN3639A AUPN363995A0 (en) 1995-06-19 1995-06-19 Electrochemical cell
AUPN3639 1995-06-19
CA002222525A CA2222525C (en) 1995-06-19 1996-06-19 Electrochemical cell

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA002222525A Division CA2222525C (en) 1995-06-19 1996-06-19 Electrochemical cell

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA002573423A Division CA2573423C (en) 1995-06-19 1996-06-19 Electrochemical cell

Publications (2)

Publication Number Publication Date
CA2538966A1 true CA2538966A1 (en) 1997-01-03
CA2538966C CA2538966C (en) 2007-11-20

Family

ID=3787980

Family Applications (3)

Application Number Title Priority Date Filing Date
CA002573423A Expired - Lifetime CA2573423C (en) 1995-06-19 1996-06-19 Electrochemical cell
CA002538966A Expired - Lifetime CA2538966C (en) 1995-06-19 1996-06-19 Electrochemical cell
CA002222525A Expired - Lifetime CA2222525C (en) 1995-06-19 1996-06-19 Electrochemical cell

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CA002573423A Expired - Lifetime CA2573423C (en) 1995-06-19 1996-06-19 Electrochemical cell

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA002222525A Expired - Lifetime CA2222525C (en) 1995-06-19 1996-06-19 Electrochemical cell

Country Status (14)

Country Link
US (1) US6284125B1 (en)
EP (3) EP1925934A3 (en)
JP (2) JP4018748B2 (en)
KR (3) KR100491283B1 (en)
CN (7) CN1184471C (en)
AT (1) ATE237131T1 (en)
AU (2) AUPN363995A0 (en)
CA (3) CA2573423C (en)
DE (2) DE69627378T2 (en)
DK (1) DK0873514T3 (en)
ES (1) ES2193242T3 (en)
HK (1) HK1107147A1 (en)
PT (1) PT873514E (en)
WO (1) WO1997000441A1 (en)

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